C10G-E020C Analytical and Testing Instruments for Food Safety Analytical and Testing Support Total Shimadzu’s Safety for Food

Shimadzu’s Total Support for Food Safety Type Region and Identification © Shimadzu Corporation, 2018 Defects in Packaging Prevention of Printed in Japan 3655-02814-10AIT Allergens Off-Flavor Analysis of Inspection and Foreign Matter Toxic Metals Food Additives Bacteria Mycotoxins For Research Use Only. Not for use in diagnostic procedures. Not for use in diagnostic procedures. Use Only. For Research Please contact us to check the availability of these not available in your country. that are to products This publication may contain references in your country. products trademarks and trade names of Shimadzu Corporation, its names and logos used in this publication are Company names, products/service used with trademark symbol “TM” or “®”. whether or not they are subsidiaries or its affiliates, whether or not to either the entities or their products/services, trademarks and trade names may be used in this publication to refer Third-party used with trademark symbol “TM” or “®”. they are in trademarks and trade names other than its own. interest Shimadzu disclaims any proprietary change without notice. Shimadzu subject to to you “as is” without warranty of any kind, and are provided The contents of this publication are to the use of this publication. relating or indirect, or liability for any damage, whether direct does not assume any responsibility Thanks to the built-in UniBloc AP integrated aluminum mass sensor and an Thanks to the built-in UniBloc AP integrated a optimized control system, this balance achieves high-speed measurements with and response as quick as 2 seconds. It has been designed for excellent operability, The AP-W series is equipped with a features an easy-to-read organic EL display. function to automatically calculate the weight values required for sample concentration preparation, which supports routine weighing operations. Veterinary Pharmaceuticals Residual Pesticides : Applicable Universal Testing Machine Universal Testing (AG, EZ Test) Gas Chromatograph (GC) Gas Chromatograph Gas Chromatograph Mass spectrometer (GC-MS) Liquid Chromatograph (LC) Liquid Chromatograph Mass spectrometer (LC-MS) MALDI-TOF Mass Spectrometer (MALDI-TOF-MS) Spectrophotometer UV-VIS (UV) FTIR Spectrophotometer (FTIR) Atomic Absorption Spectrophotometer (AA) ICP Emission / Spectrometer ICP-MS) (ICP, X-Ray Fluorescence EDX) Spectrometer (XRF, List of Analytical and Testing Instruments for Food Safety for Food Instruments and Testing List of Analytical Shimadzu Balances 〇 www.shimadzu.com/an/ Shimadzu's Total Support for Food Safety

Shimadzu provides total support for food safety, whether it is farm to World Map of Shimadzu Sales, Service, fork or bait to plate. As a leading manufacturer of a wide range of Manufacturing, and R&D Facilities analytical instruments, Shimadzu undertakes development of new instruments and technologies, and provides comprehensive service support in order to keep up with changing market demands.

Item Target Instruments Used Reference Page Contents

Contaminants Residual Pesticides Liquid Chromatograph Mass Spectrometer P. 4

Gas Chromatograph Mass Spectrometer P. 5

Multi-Analyte Quantitation Software P. 5

Liquid Chromatograph P. 6

Gas Chromatograph P. 6

Veterinary Pharmaceuticals Liquid Chromatograph Mass Spectrometer P. 7

Mycotoxins Liquid Chromatograph P. 8

Liquid Chromatograph Mass Spectrometer P. 8

Microorganisms MALDI-TOF Mass Spectrometer P. 9 Toxic Metals Atomic Absorption Spectrophotometer P. 10

ICP Atomic Emission Spectrometer P. 10

Food Additives Preservatives UV-VIS Spectrophotometer P. 11

Coloring Agents Liquid Chromatograph P. 11

Flavor Compounds Gas Chromatograph Mass Spectrometer P. 12

Sweeteners Liquid Chromatograph Mass Spectrometer P. 12

Processing / Acrylamide Liquid Chromatograph Mass Spectrometer P. 13 Packaging Allergens Liquid Chromatograph Mass Spectrometer P. 14

Off-Flavor Gas Chromatograph Mass Spectrometer P. 15

Foreign Matter Energy Dispersive X-ray Fluorescence Spectrometer P. 16 Sales and Service FTIR Spectrophotometer P. 16 Manufacturing Software for Integrated Analysis of EDX and FTIR Data P. 16 R&D Adhesive Strength of Packaging Universal Testing Machine P. 17 MOSH/MOAH LC-GC Online System P. 17

Others Halal Testing Liquid Chromatograph Mass Spectrometer P. 18

Laboratory Data Management Network-Compatible Analysis Data System P. 19 Shimadzu's Total Support for Food Safety

Shimadzu provides total support for food safety, whether it is farm to World Map of Shimadzu Sales, Service, fork or bait to plate. As a leading manufacturer of a wide range of Manufacturing, and R&D Facilities analytical instruments, Shimadzu undertakes development of new instruments and technologies, and provides comprehensive service support in order to keep up with changing market demands.

Item Target Instruments Used Reference Page Contents

Contaminants Residual Pesticides Liquid Chromatograph Mass Spectrometer P. 4

Gas Chromatograph Mass Spectrometer P. 5

Multi-Analyte Quantitation Software P. 5

Liquid Chromatograph P. 6

Gas Chromatograph P. 6

Veterinary Pharmaceuticals Liquid Chromatograph Mass Spectrometer P. 7

Mycotoxins Liquid Chromatograph P. 8

Liquid Chromatograph Mass Spectrometer P. 8

Microorganisms MALDI-TOF Mass Spectrometer P. 9 Toxic Metals Atomic Absorption Spectrophotometer P. 10

ICP Atomic Emission Spectrometer P. 10

Food Additives Preservatives UV-VIS Spectrophotometer P. 11

Coloring Agents Liquid Chromatograph P. 11

Flavor Compounds Gas Chromatograph Mass Spectrometer P. 12

Sweeteners Liquid Chromatograph Mass Spectrometer P. 12

Processing / Acrylamide Liquid Chromatograph Mass Spectrometer P. 13 Packaging Allergens Liquid Chromatograph Mass Spectrometer P. 14

Off-Flavor Gas Chromatograph Mass Spectrometer P. 15

Foreign Matter Energy Dispersive X-ray Fluorescence Spectrometer P. 16 Sales and Service FTIR Spectrophotometer P. 16 Manufacturing Software for Integrated Analysis of EDX and FTIR Data P. 16 R&D Adhesive Strength of Packaging Universal Testing Machine P. 17 MOSH/MOAH LC-GC Online System P. 17

Others Halal Testing Liquid Chromatograph Mass Spectrometer P. 18

Laboratory Data Management Network-Compatible Analysis Data System P. 19 Analysis of Residual Pesticides Analysis of Residual Pesticides

Food knows no borders. With an increasing global population, food security is increasingly under threat and there is a Simultaneous Analysis of Residual Pesticides (GC-MS/MS) Triple Quadrupole Mass Spectrometer growing challenge for agriculture to produce more food, safely and more sustainably. The use of herbicides, insecticides, and GCMS-TQ8050 (×1,000,000) fungicides reduce crop losses both before and after harvest, and increase crop yields. However, pesticide residues resulting 7.0

from the use of plant protection products on crops may pose a risk to human health; as a result, regulatory guidelines have 6.0

been implemented to monitor pesticide residues in food. Shimadzu offers a wide range of instruments for pesticide testing, 5.0

from GC and LC with specific detectors to single and triple quadrupole mass spectrometers. 4.0

3.0

2.0

1.0 Triple Quadrupole Mass Spectrometer Simultaneous Analysis of Residual Pesticides (LC-MS/MS) LCMS-8060 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 LC-MS/MS MRM (Multiple Reaction Monitoring) analysis allows Simultaneous Analysis of 439 Pesticides Using GC-MS/MS with Smart MRM for rapid and sensitive detection of pesticides in complex matrices. An example of simultaneous multi-residue multi-class Source: Shimadzu Application Data Sheet No. C106, 107 pesticide analysis is shown below. Finally, a triple quadrupole GC-MS/MS Smart enough for everyday use in your laboratory. The Shimadzu GCMS-TQ8050 is the (x1,000,000) first triple quadrupole with Smart Productivity for high-efficiency sample throughput, Smart Operation for quick and easy 2.00 method development, and Smart Performance for low detection limits and Scan/MRM. These three smart technologies

1.75 contribute to Smart MRM and provide the most accurate, cost-effective, and easy-to-use triple quadrupole GCMS you have ever imagined. 1.50 1.25 Database 1.00

The LCMS-8060 is a groundbreaking innovation in mass 0.75 Database for Quantitative Analysis Smart Pesticides Database spectrometry, helping to transform LC-MS/MS data quality by 0.50 The Smart Pesticides Database includes optimized transitions and developing a new vision of sensitivity and speed. 0.25 collision energies, CAS registry numbers, and Retention Indices (RI) Shimadzu is the first mass spectrometry company in the 0 for hundreds of pesticides. Select from pre-registered compounds in 0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 min world to deliver a triple quadrupole mass spectrometer with one database, or add you own optimized transitions. Select the the highest sensitivity and ultra-fast technologies. Simultaneous Analysis of 646 Pesticides (10ng/mL) within 10.5 minutes Using LC-MS/MS compounds to be analyzed, and Smart MRM builds the MRM or Scan/MRM acquisition method from the “Smart Database” with a push of a button.

Database Peak Area Response Quantitation Ion Database for Screening Analysis ×106 LC/MS/MS Method Package for Residual Pesticides 5.0 APPLE MINT TOMATO Quick-DB*

4.5 The Method Package covers 836 components subject to EU Directive %RSD 3.7 MS Residual Pesticides Database No. 396/2005, Chinese GB standards, and the Japanese Multi-residue 4.0 In pesticide analysis, increased purchasing costs and the control %RSD 3.4 %RSD 3.4 Dipropetryn Method for Agricultural Chemicals by LC-MS/MS. In the USA, the EPA 3.5 operations associated with standard samples have become issues for sets the pesticide limits while the FDA and USDA enforce those limits. 3.0 the industry. The Quick-DB contains information on 491 The method package also includes optimized analytical conditions, 2.5 components, optimized analytical conditions, and calibration curves %RSD 3.9 2.0 created using pesticide surrogates as internal standard substances. which enables simultaneous analysis of 646 pesticides in 10.5 minutes %RSD 3.2 Probenazole without performing a complicated conditions setting process. 1.5 %RSD 3.6 This allows quick screening of residual pesticides without using 1.0 %RSD 5.4 %RSD 5.9 Thiofanox-sulfone pesticide standard samples. %RSD 5.8 0.5 Registered *Not available in the USA Method File Mode 0.0 Component 0 50 100 150 200 250 300 Injections number Pesticide MRM 836 Thiofanox-sulfone Dipropetryn Probenazole Multi-Analyte Quantitation Software

Peak Area Response for Three Pesticides Spiked into Apple, Mint and Tomato LabSolutions Insight Matrix Extracts at 0.05 mg/kg over 72 Hours. Due to increasingly sophisticated automation, large quantities Source: Shimadzu Application News No. C136 of high-quality mass spectrometer data can be obtained. However, checking and analyzing all that data can be extremely time-consuming, which significantly decreases the efficiency of inspection work. LabSolutions Insight multi-analyte quantitation software for GC-MS/MS and LC-MS/MS analysis helps analyze such multi-analyte data more conveniently and more efficiently.

Shimadzu’s Total Support for Food Safety 4 Analytical and Testing Instruments for Food Safety 5 Analysis of Residual Pesticides Analysis of Residual Pesticides

Food knows no borders. With an increasing global population, food security is increasingly under threat and there is a Simultaneous Analysis of Residual Pesticides (GC-MS/MS) Triple Quadrupole Mass Spectrometer growing challenge for agriculture to produce more food, safely and more sustainably. The use of herbicides, insecticides, and GCMS-TQ8050 (×1,000,000) fungicides reduce crop losses both before and after harvest, and increase crop yields. However, pesticide residues resulting 7.0 from the use of plant protection products on crops may pose a risk to human health; as a result, regulatory guidelines have 6.0 been implemented to monitor pesticide residues in food. Shimadzu offers a wide range of instruments for pesticide testing, 5.0 from GC and LC with specific detectors to single and triple quadrupole mass spectrometers. 4.0

3.0

2.0

1.0 Triple Quadrupole Mass Spectrometer Simultaneous Analysis of Residual Pesticides (LC-MS/MS) LCMS-8060 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 LC-MS/MS MRM (Multiple Reaction Monitoring) analysis allows Simultaneous Analysis of 439 Pesticides Using GC-MS/MS with Smart MRM for rapid and sensitive detection of pesticides in complex matrices. An example of simultaneous multi-residue multi-class Source: Shimadzu Application Data Sheet No. C106, 107 pesticide analysis is shown below. Finally, a triple quadrupole GC-MS/MS Smart enough for everyday use in your laboratory. The Shimadzu GCMS-TQ8050 is the (x1,000,000) first triple quadrupole with Smart Productivity for high-efficiency sample throughput, Smart Operation for quick and easy 2.00 method development, and Smart Performance for low detection limits and Scan/MRM. These three smart technologies

1.75 contribute to Smart MRM and provide the most accurate, cost-effective, and easy-to-use triple quadrupole GCMS you have ever imagined. 1.50 1.25 Database 1.00

The LCMS-8060 is a groundbreaking innovation in mass 0.75 Database for Quantitative Analysis Smart Pesticides Database spectrometry, helping to transform LC-MS/MS data quality by 0.50 The Smart Pesticides Database includes optimized transitions and developing a new vision of sensitivity and speed. 0.25 collision energies, CAS registry numbers, and Retention Indices (RI) Shimadzu is the first mass spectrometry company in the 0 for hundreds of pesticides. Select from pre-registered compounds in 0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 min world to deliver a triple quadrupole mass spectrometer with one database, or add you own optimized transitions. Select the the highest sensitivity and ultra-fast technologies. Simultaneous Analysis of 646 Pesticides (10ng/mL) within 10.5 minutes Using LC-MS/MS compounds to be analyzed, and Smart MRM builds the MRM or Scan/MRM acquisition method from the “Smart Database” with a push of a button.

Database Peak Area Response Quantitation Ion Database for Screening Analysis ×106 LC/MS/MS Method Package for Residual Pesticides 5.0 APPLE MINT TOMATO Quick-DB*

4.5 The Method Package covers 836 components subject to EU Directive %RSD 3.7 MS Residual Pesticides Database No. 396/2005, Chinese GB standards, and the Japanese Multi-residue 4.0 In pesticide analysis, increased purchasing costs and the control %RSD 3.4 %RSD 3.4 Dipropetryn Method for Agricultural Chemicals by LC-MS/MS. In the USA, the EPA 3.5 operations associated with standard samples have become issues for sets the pesticide limits while the FDA and USDA enforce those limits. 3.0 the industry. The Quick-DB contains information on 491 The method package also includes optimized analytical conditions, 2.5 components, optimized analytical conditions, and calibration curves %RSD 3.9 2.0 created using pesticide surrogates as internal standard substances. which enables simultaneous analysis of 646 pesticides in 10.5 minutes %RSD 3.2 Probenazole without performing a complicated conditions setting process. 1.5 %RSD 3.6 This allows quick screening of residual pesticides without using 1.0 %RSD 5.4 %RSD 5.9 Thiofanox-sulfone pesticide standard samples. %RSD 5.8 0.5 Registered *Not available in the USA Method File Mode 0.0 Component 0 50 100 150 200 250 300 Injections number Pesticide MRM 836 Thiofanox-sulfone Dipropetryn Probenazole Multi-Analyte Quantitation Software

Peak Area Response for Three Pesticides Spiked into Apple, Mint and Tomato LabSolutions Insight Matrix Extracts at 0.05 mg/kg over 72 Hours. Due to increasingly sophisticated automation, large quantities Source: Shimadzu Application News No. C136 of high-quality mass spectrometer data can be obtained. However, checking and analyzing all that data can be extremely time-consuming, which significantly decreases the efficiency of inspection work. LabSolutions Insight multi-analyte quantitation software for GC-MS/MS and LC-MS/MS analysis helps analyze such multi-analyte data more conveniently and more efficiently.

Shimadzu’s Total Support for Food Safety 4 Analytical and Testing Instruments for Food Safety 5 Analysis of Residual Pesticides Analysis of Veterinary Pharmaceuticals

Gas Chromatograph (GC) Liquid Chromatograph (LC) Veterinary pharmaceuticals are used in the diagnosis, cure, mitigation, treatment, and prevention of diseases in animals as well as in the promotion of growth. However, there is increasing concern about these drugs entering the food chain and their Capillary GC can be utilized for the analysis of residual GC or GC-MS techniques may not be suitable for analysis of effects on the human body. These drugs are categorized in many classes, such as anabolic hormones, anthelmintics, pesticides in foods. Using a selective detector such as FPD, residual pesticides that thermally decompose or have a antibiotics, beta-agonists, coccidiostats, corticosteroids, nonsteroidal anti-inflammatory drugs (NSAIDs), and sedatives. Below FTD or ECD offers highly sensitive analysis of trace pesticides. highly polar chemical structure. In contrast, an HPLC are a few examples of veterinary pharmaceutical analyses. equipped with a photodiode array detector can acquire reliable chromatograms with spectral information for building libraries to compare compounds. The Shimadzu carbamate analysis system incorporates derivatization fenthion methamidophos

fosthiazate 2 + prothiofos technologies to achieve high-sensitivity, selective analysis of Analysis of Multiple Veterinary Pharmaceuticals in Pork (LC-MS/MS) (Z)-chlorfenvinphos malathion pirimiphos-methyl thiometon salithion (E)-dimethylvinphos cyanophos (Z)-dimethylvinphos

diazinon N-methyl carbamate pesticides. terbufos

chlorpyrifos-methyl + dimethoate Veterinary pharmaceuticals are often used to promote the growth of farm animals, but many of these compounds are subject iprobenfos dichlorvos dichlofenthion formothion acephate

phenthoate to residue standards. From this background, there is a need for a highly sensitive and rapid analytical technique to analyze as isophenphos + quinalphos

etrimfos mV ethion + sulprofos profenofos + methidathion pyridaphenthion ethoprophos tolclofos-methyl chlorpyrifos fenitrothion (MEP)

phorate many of these compounds as possible in a single run. LC-MS/MS enables simultaneous analysis of multiple veterinary EPN 60 cadusafos cyanofenphos fensulfothion (E)-chlorfenvinphos propaphos butamifos edifenphos pharmaceuticals with high sensitivity. phosmet phosalone fosthiazate pyraclofos

50 Aldicarb Oxamyl

Pesticide-spiked meat dumpling sample (0.2 µ g/g equivalent) Methomyl Triple Quadrupole Mass Spectrometer 40 Normal meat dumpling sample

Carbaryl LCMS-8060 Methiocarb sulfoxide Ethiofencarb 30 Bendiocarb Chromatograms of Normal Meat Sample and Pesticides-spiked Meat Sample Methiocarb sulfone Fenobucarb

20 Methiocarb

Gas Chromatograph Nexis GC-2030 10

0

0.0 5.0 10.0 15.0 20.0 25.0 min Chromatogram of a Standard Mixture of 10 N-Methylcarbamate Pesticides MRM Chromatograms of 89 Veterinary Pharmaceuticals in Pork

High-Performance Liquid Chromatograph Source: Shimadzu Application News No. C99 Prominence Series Carbamates Pesticides Analysis System Analysis of Veterinary Pharmaceuticals Using MRM Spectrum Mode (LC-MS/MS) MRM Spectrum Mode was developed to reduce false positives and false negatives in analysis of veterinary pharmaceuticals. MRM Spectrum Mode acquires a high number of fragment ion transitions for each target compound and generates fragmentation spectra. In examination of quantifying and identifying 212 veterinary pharmaceuticals, the level of confidence in compound identification and verification increased in MRM Spectrum Mode, and limits of detection, linearity or Features repeatability were not compromised compared to a conventional method (2 MRM / compound). Easy Operation An advanced interface enables intuitive operation with clear graphics. Shimadzu's latest tool-free maintenance technology makes daily maintenance easy. Features High Sensitivity and Reproducibility Achieves high sensitivity with all of the detectors, such as FID and Rapid Injection BID. The advanced flow controller (AFC) enhances reliability with An injection time of 10 seconds for a 10 µL sample enhances analysis excellent repeatability. efficiency. Exceptional Expandability and Productivity High Sensitivity and Linearity over a Broad Range Nexis GC-2030 can be customized to meet customers' specific Upgraded optical and electrical systems reduce noise for lower requirements and needs. Options and functions to use hydrogen detection limits and ensure simultaneous analysis of major carrier gas safely in high-speed analysis maximize analysis components and trace components. productivity. Low Sample Carryover Platinum coating minimizes the adsorption of sample components

on to the needle surface. MRM Spectra and Calibration Curve of Sulfamerazine (1 pg/µL to 100 pg/µL)

Source: Shimadzu Application News No. C161

Shimadzu’s Total Support for Food Safety 6 Analytical and Testing Instruments for Food Safety 7 Analysis of Residual Pesticides Analysis of Veterinary Pharmaceuticals

Gas Chromatograph (GC) Liquid Chromatograph (LC) Veterinary pharmaceuticals are used in the diagnosis, cure, mitigation, treatment, and prevention of diseases in animals as well as in the promotion of growth. However, there is increasing concern about these drugs entering the food chain and their Capillary GC can be utilized for the analysis of residual GC or GC-MS techniques may not be suitable for analysis of effects on the human body. These drugs are categorized in many classes, such as anabolic hormones, anthelmintics, pesticides in foods. Using a selective detector such as FPD, residual pesticides that thermally decompose or have a antibiotics, beta-agonists, coccidiostats, corticosteroids, nonsteroidal anti-inflammatory drugs (NSAIDs), and sedatives. Below FTD or ECD offers highly sensitive analysis of trace pesticides. highly polar chemical structure. In contrast, an HPLC are a few examples of veterinary pharmaceutical analyses. equipped with a photodiode array detector can acquire reliable chromatograms with spectral information for building libraries to compare compounds. The Shimadzu carbamate analysis system incorporates derivatization fenthion methamidophos fosthiazate 2 + prothiofos technologies to achieve high-sensitivity, selective analysis of Analysis of Multiple Veterinary Pharmaceuticals in Pork (LC-MS/MS) (Z)-chlorfenvinphos malathion pirimiphos-methyl thiometon salithion (E)-dimethylvinphos cyanophos (Z)-dimethylvinphos diazinon N-methyl carbamate pesticides. terbufos chlorpyrifos-methyl + dimethoate Veterinary pharmaceuticals are often used to promote the growth of farm animals, but many of these compounds are subject iprobenfos dichlorvos dichlofenthion formothion acephate

phenthoate to residue standards. From this background, there is a need for a highly sensitive and rapid analytical technique to analyze as isophenphos + quinalphos

etrimfos mV ethion + sulprofos profenofos + methidathion pyridaphenthion ethoprophos tolclofos-methyl chlorpyrifos fenitrothion (MEP) phorate many of these compounds as possible in a single run. LC-MS/MS enables simultaneous analysis of multiple veterinary EPN 60 cadusafos cyanofenphos fensulfothion (E)-chlorfenvinphos propaphos butamifos edifenphos pharmaceuticals with high sensitivity. phosmet phosalone fosthiazate pyraclofos

50 Aldicarb Oxamyl

Pesticide-spiked meat dumpling sample (0.2 µ g/g equivalent) Methomyl Triple Quadrupole Mass Spectrometer 40 Normal meat dumpling sample

Carbaryl LCMS-8060 Methiocarb sulfoxide Ethiofencarb 30 Bendiocarb Chromatograms of Normal Meat Sample and Pesticides-spiked Meat Sample Methiocarb sulfone Fenobucarb

20 Methiocarb

Gas Chromatograph Nexis GC-2030 10

0

0.0 5.0 10.0 15.0 20.0 25.0 min Chromatogram of a Standard Mixture of 10 N-Methylcarbamate Pesticides MRM Chromatograms of 89 Veterinary Pharmaceuticals in Pork

High-Performance Liquid Chromatograph Source: Shimadzu Application News No. C99 Prominence Series Carbamates Pesticides Analysis System Analysis of Veterinary Pharmaceuticals Using MRM Spectrum Mode (LC-MS/MS) MRM Spectrum Mode was developed to reduce false positives and false negatives in analysis of veterinary pharmaceuticals. MRM Spectrum Mode acquires a high number of fragment ion transitions for each target compound and generates fragmentation spectra. In examination of quantifying and identifying 212 veterinary pharmaceuticals, the level of confidence in compound identification and verification increased in MRM Spectrum Mode, and limits of detection, linearity or Features repeatability were not compromised compared to a conventional method (2 MRM / compound). Easy Operation An advanced interface enables intuitive operation with clear graphics. Shimadzu's latest tool-free maintenance technology makes daily maintenance easy. Features High Sensitivity and Reproducibility Achieves high sensitivity with all of the detectors, such as FID and Rapid Injection BID. The advanced flow controller (AFC) enhances reliability with An injection time of 10 seconds for a 10 µL sample enhances analysis excellent repeatability. efficiency. Exceptional Expandability and Productivity High Sensitivity and Linearity over a Broad Range Nexis GC-2030 can be customized to meet customers' specific Upgraded optical and electrical systems reduce noise for lower requirements and needs. Options and functions to use hydrogen detection limits and ensure simultaneous analysis of major carrier gas safely in high-speed analysis maximize analysis components and trace components. productivity. Low Sample Carryover Platinum coating minimizes the adsorption of sample components on to the needle surface. MRM Spectra and Calibration Curve of Sulfamerazine (1 pg/µL to 100 pg/µL)

Source: Shimadzu Application News No. C161

Shimadzu’s Total Support for Food Safety 6 Analytical and Testing Instruments for Food Safety 7 Analysis of Mycotoxins Identification and Observation of Microorganisms

Mycotoxins are chemical products produced in fungus and are toxic to humans, animals, and crops. There are more than 300 In the event of food poisoning, identification of the causative factor is extremely important in determining the path of known mycotoxins, including aflatoxin, deoxynivalenol, patulin, fumonisin and others, and some of them are strictly infection, prescribing treatment and preventing recurrence. Shimadzu’s iDPlus microorganism identification platform provides controlled under regulations. HPLC and LC-MS are mainly used for analysis of mycotoxins, and simultaneous analysis of rapid identification of microorganisms in food. multiple mycotoxins has been carried out for more efficient screening. Identification of Microorganisms (MALDI-TOF-MS) AXIMA Microorganism Identification System In order to prevent pathogenic germs from contaminating Analysis of Aflatoxins (LC) Rapid Screening of 10 Mycotoxins (LC) food sources, it is essential to identify them quickly and The analysis of aflatoxins (B1, B2, G1 and G2) is very Simultaneous analysis of mycotoxins is important because reliably in order to allow for proper controlled measures. important from a food safety perspective due to concerns multiple mycotoxins have been detected in a single food and A microorganism identification system using the AXIMA about adverse effects on human health. The Nexera Ultra may cause a synergistic effect. From this background, a MALDI-TOF MS enables direct measurement of cultured High Performance Liquid Chromatograph achieves simultaneous analytical method has been developed for microorganisms and identification within two minutes. high-resolution analysis of aflatoxins in less than four rapid screening of 10 mycotoxins using HPLC. Shown below % Int. minutes. The RF-20Axs high-sensitivity fluorescence detector are chromatograms of 10 mycotoxins by fluorescence (upper) Salmonella enterica subsp. Enterica allows direct analysis of aflatoxins at trace levels (ppt) and PDA (lower) detectors without derivatization. 5369.13 6091.59 without any derivatization. 4365.39 mV 3 10950.18 RF-20AXS Peaks 7658.66 Integrated HPLC 4 ■ 7158.16 1) AFM1 1 µg/L 3829.04 2 Peaks Nexera-i 2) AFG2 0.25 µg/L 9520.27

1 AFG2 (0.6 µg/kg) 2 3) AFG1 0.25 µg/L 6885.93 2 AFG1 (2.4 µg/kg) 1 4) AFB2 0.25 µg/L 3 AFB2 (0.6 µg/kg) 5) AFB1 0.25 µg/L Staphylococcus aureus subsp. Aureus 4 AFB1 (2.4 µg/kg) 6) ZON 50 µg/L 5033.17 1 7) OTA 5 µg/L 5524.87 4308.68

7 6352.79 5 3 6 12397.15 13762.64 0 3640.52

Pseudomonas aeruginosa 4 5 6 7 8 9 10 11 6043.95 6671.91 5209.27 min 7230.02 8348.89 4434.57

mAU 7886.39 11424.07 Nexera-i PDA 7590.49

Peaks 12295.04 ■ 4054.41 10220.70 11027.94 13698.41 11922.76 14991.82 12706.04 14067.89 5 8) NIV 1000 µg/L 13225.69 8 9) DON 1000 µg/L 10) PAT 25 µg/L Escherichia coli

9 7156.44 6253.14

9059.12 Features 9733.78

Analysis of Aflatoxins without TFA Derivatization（ N = 5） 7867.55 0 10 220 nm 8364.74 276 nm 8871.39 Quick and Easy Analysis Source: Shimadzu Application News No. L435 13645.06 Three steps from sample preparation to identification. 0.0 0.5 1.0 1.5 2.0 2.5 3.0 Bacillus subtilis min The microorganism identification process is finished within two Simultaneous Analysis of 10 Mycotoxins 4307.86 5255.03 9235.80 3890.60 minutes after starting the analysis. Simultaneous Analysis of 25 Mycotoxins (LC-MS/MS) Source: Shimadzu Application News No. L512 Highly Accurate Identification 4000 6000 8000 10000 12000 14000 LC-MS/MS is effective for analysis of mycotoxins in complex m/z Microorganism identification by MALDI-TOF MS is a well-established technique with a low number of false positives. matrices. Shown is an example of the simultaneous analysis Comparing mass spectra of different bacteria of 25 mycotoxins by LC-MS/MS and the Method Package for Applicable for a Wide Range of Microorganisms Database Source: https://www.shimadzu.com/an/industry/pharmaceuticallifescience/proteome0207005.htm Mycotoxins. All compounds, including positive and negative Capable of classifying/identifying gram-positive and gram-negative ions, were separated and detected with high sensitivity LC/MS/MS Method Package for Mycotoxins bacteria, yeasts, fungi and spores. iDPlus identifies mircoorganisms What is Microorganism Identification by MALDI-TOF MS? by family, genus, species, and subspecies. within 12 minutes. For analysis of mycotoxins with high The Method Package provides simultaneous multi-component analysis conditions for mycotoxins subject to regulations in the US, the EU, Species-specific mass spectral patterns from microorganisms can be Database Customization sensitivity, eliminating carryover is indispensable. Modified China and Japan. It contains seven different analytical method obtained by direct measurement of microorganisms using MALDI-TOF Easily register new microorganisms in the database, which allows rinse methods using the SIL-30AC autosampler can eliminate conditions and fully optimized MRM transitions for a total of 27 MS. Identification of microorganisms can be performed by matching rapid development of a high-quality database. carryover of fumonisins. Low Operational Cost different mycotoxins. Rapid analytical methods are included for a 25 these collected spectra to the database. This is a fast, easy and mycotoxin screen, 16 mycotoxins using high-sensitivity conditions, 10 Analysis can be performed for pennies per sample compared to reliable method for identification of microorganisms. mycotoxins using APCI, and specialized methods for trichothecenes, expensive traditional techniques. fumonisins, aflatoxins, and patulin. Expanded Research Capabilities iDPlus is not limited to microorganism identification. Reflectron mode and MS/MS functionalities enable a true research platform. Number of Analysis Method Components Time (min) Simultaneous Analysis of 25 Mycotoxins 25 15 Simultaneous Analysis with High Sensitivity 16 30 Patulin Analysis 1 10 Aflatoxins Analysis 6 30 Trichothecenes Analysis 4 12 High-speed Simultaneous Analysis of 25 Mycotoxins Fumonisins Analysis 3 15

Source: Shimadzu Flyer No. C146-E351 10 mycotoxins using APCI 10 33

Shimadzu’s Total Support for Food Safety 8 Analytical and Testing Instruments for Food Safety 9 Analysis of Mycotoxins Identification and Observation of Microorganisms

Mycotoxins are chemical products produced in fungus and are toxic to humans, animals, and crops. There are more than 300 In the event of food poisoning, identification of the causative factor is extremely important in determining the path of known mycotoxins, including aflatoxin, deoxynivalenol, patulin, fumonisin and others, and some of them are strictly infection, prescribing treatment and preventing recurrence. Shimadzu’s iDPlus microorganism identification platform provides controlled under regulations. HPLC and LC-MS are mainly used for analysis of mycotoxins, and simultaneous analysis of rapid identification of microorganisms in food. multiple mycotoxins has been carried out for more efficient screening. Identification of Microorganisms (MALDI-TOF-MS) AXIMA Microorganism Identification System In order to prevent pathogenic germs from contaminating Analysis of Aflatoxins (LC) Rapid Screening of 10 Mycotoxins (LC) food sources, it is essential to identify them quickly and The analysis of aflatoxins (B1, B2, G1 and G2) is very Simultaneous analysis of mycotoxins is important because reliably in order to allow for proper controlled measures. important from a food safety perspective due to concerns multiple mycotoxins have been detected in a single food and A microorganism identification system using the AXIMA about adverse effects on human health. The Nexera Ultra may cause a synergistic effect. From this background, a MALDI-TOF MS enables direct measurement of cultured High Performance Liquid Chromatograph achieves simultaneous analytical method has been developed for microorganisms and identification within two minutes. high-resolution analysis of aflatoxins in less than four rapid screening of 10 mycotoxins using HPLC. Shown below % Int. minutes. The RF-20Axs high-sensitivity fluorescence detector are chromatograms of 10 mycotoxins by fluorescence (upper) Salmonella enterica subsp. Enterica allows direct analysis of aflatoxins at trace levels (ppt) and PDA (lower) detectors without derivatization. 5369.13 6091.59 without any derivatization. 4365.39 mV 3 10950.18 RF-20AXS Peaks 7658.66 Integrated HPLC 4 ■ 7158.16 1) AFM1 1 µg/L 3829.04 2 Peaks Nexera-i 2) AFG2 0.25 µg/L 9520.27

1 AFG2 (0.6 µg/kg) 2 3) AFG1 0.25 µg/L 6885.93 2 AFG1 (2.4 µg/kg) 1 4) AFB2 0.25 µg/L 3 AFB2 (0.6 µg/kg) 5) AFB1 0.25 µg/L Staphylococcus aureus subsp. Aureus 4 AFB1 (2.4 µg/kg) 6) ZON 50 µg/L 5033.17 1 7) OTA 5 µg/L 5524.87 4308.68

7 6352.79 5 3 6 12397.15 13762.64 0 3640.52

Pseudomonas aeruginosa 4 5 6 7 8 9 10 11 6043.95 6671.91 5209.27 min 7230.02 8348.89 4434.57 mAU 7886.39 11424.07 Nexera-i PDA 7590.49

Peaks 12295.04 ■ 4054.41 10220.70 11027.94 13698.41 11922.76 14991.82 12706.04 14067.89 5 8) NIV 1000 µg/L 13225.69 8 9) DON 1000 µg/L 10) PAT 25 µg/L Escherichia coli

9 7156.44 6253.14

9059.12 Features 9733.78

Analysis of Aflatoxins without TFA Derivatization（ N = 5） 7867.55 0 10 220 nm 8364.74 276 nm 8871.39 Quick and Easy Analysis Source: Shimadzu Application News No. L435 13645.06 Three steps from sample preparation to identification. 0.0 0.5 1.0 1.5 2.0 2.5 3.0 Bacillus subtilis min The microorganism identification process is finished within two Simultaneous Analysis of 10 Mycotoxins 4307.86 5255.03 9235.80 3890.60 minutes after starting the analysis. Simultaneous Analysis of 25 Mycotoxins (LC-MS/MS) Source: Shimadzu Application News No. L512 Highly Accurate Identification 4000 6000 8000 10000 12000 14000 LC-MS/MS is effective for analysis of mycotoxins in complex m/z Microorganism identification by MALDI-TOF MS is a well-established technique with a low number of false positives. matrices. Shown is an example of the simultaneous analysis Comparing mass spectra of different bacteria of 25 mycotoxins by LC-MS/MS and the Method Package for Applicable for a Wide Range of Microorganisms Database Source: https://www.shimadzu.com/an/industry/pharmaceuticallifescience/proteome0207005.htm Mycotoxins. All compounds, including positive and negative Capable of classifying/identifying gram-positive and gram-negative ions, were separated and detected with high sensitivity LC/MS/MS Method Package for Mycotoxins bacteria, yeasts, fungi and spores. iDPlus identifies mircoorganisms What is Microorganism Identification by MALDI-TOF MS? by family, genus, species, and subspecies. within 12 minutes. For analysis of mycotoxins with high The Method Package provides simultaneous multi-component analysis conditions for mycotoxins subject to regulations in the US, the EU, Species-specific mass spectral patterns from microorganisms can be Database Customization sensitivity, eliminating carryover is indispensable. Modified China and Japan. It contains seven different analytical method obtained by direct measurement of microorganisms using MALDI-TOF Easily register new microorganisms in the database, which allows rinse methods using the SIL-30AC autosampler can eliminate conditions and fully optimized MRM transitions for a total of 27 MS. Identification of microorganisms can be performed by matching rapid development of a high-quality database. carryover of fumonisins. Low Operational Cost different mycotoxins. Rapid analytical methods are included for a 25 these collected spectra to the database. This is a fast, easy and mycotoxin screen, 16 mycotoxins using high-sensitivity conditions, 10 Analysis can be performed for pennies per sample compared to reliable method for identification of microorganisms. mycotoxins using APCI, and specialized methods for trichothecenes, expensive traditional techniques. fumonisins, aflatoxins, and patulin. Expanded Research Capabilities iDPlus is not limited to microorganism identification. Reflectron mode and MS/MS functionalities enable a true research platform. Number of Analysis Method Components Time (min) Simultaneous Analysis of 25 Mycotoxins 25 15 Simultaneous Analysis with High Sensitivity 16 30 Patulin Analysis 1 10 Aflatoxins Analysis 6 30 Trichothecenes Analysis 4 12 High-speed Simultaneous Analysis of 25 Mycotoxins Fumonisins Analysis 3 15

Source: Shimadzu Flyer No. C146-E351 10 mycotoxins using APCI 10 33

Shimadzu’s Total Support for Food Safety 8 Analytical and Testing Instruments for Food Safety 9 Analysis of Toxic Metals Analysis of Food Additives

Toxic metals (arsenic, lead, cadmium, etc.) in foods must be controlled down to low concentration levels. A wide variety of additives, such as preservatives, flavor enhancers, coloring agents and antioxidants, are used on foods. Shimadzu atomic absorption spectrophotometers and ICP emission spectrometers offer highly sensitive analysis of trace Strict monitoring of food stuffs is essential to detect the use of prohibited chemicals. Each country assigns a unique number or elements in foods. Shimadzu’s product lineup also includes X-ray fluorescence spectrometers that can perform elemental code of approved chemicals for that country. Shimadzu offers a wide range of products for detecting the prohibited analysis without sample pretreatment. These instruments are ideal for screening analysis and emergency analysis. chemicals, whether they were added intentionally or through contamination.

Analysis of Cd and Pb in Food Additives (AA) Atomic Absorption Spectrophotometer AA-7000 Analysis of Dicyandiamide and Melamine in Milk Powders (LC-MS/MS)

Shown here is an analysis of cadmium (Cd) and lead (Pb) in Melamine was found to be used as a protein-rich adulterant residues were found in milk products from New Zealand. α-cyclodextrin (cyclic oligosaccharide), a substance used in in pet food in 2007, and then in infant formula in 2008 in Instead of being added directly as an adulterant, the trace functional foods, pharmaceuticals, cosmetics, etc. The China. The outbreak of the melamine scandal that killed DCD, which is used for promote the growth of cow pastures, analysis was conducted by the AA-7000 atomic absorption many dogs and cats as well as led to the deaths of six infants found in milk products was explained to be relating to the

Abs Abs and illness of many others caused panic and great concerns grass “contaminated by DCD”. Cd Pb worldwide. Recently, low levels of dicyandiamide (DCD) (x100) (x1,000) 4.5 STD 1 µg/L 6.0 Area (x10,000) Area (x100,000) DCD 4.0 Melamine STD 10 µg/L (85.1>68.1) (127.1>85.1) 3.5 5.0 3.5 7.5 DCD (85.1>68.1) Melamine (127.1>85.1) 3.0 R2 = 0.997 R2 = 0.999 4.0 3.0 Sample Sample 2.5 2.5 w/Spike w/Spike 5.0 3.0 2.0 2.0 Measurement Results of Cd and Pb in α-cyclodextrin 1.5 2.0 1.5 Sample Element Cd Pb 2.5 1.0 1.0 Sample w/o Spike 1.0 0.5 w/o Spike Measured value <0.003 µg/g <0.07 µg/g 0.5 0.0 0.0 Spike and recovery rate 105 % 99 % 0.0 0.0 0 3sec 0 3sec 0.0 2.5 5.0 7.5 Conc. 0.0 2.5 5.0 7.5 Conc. 2.00 2.25 2.50 2.75 min 5.5 6.0 6.5 min Peak Profiles of Cd and Pb Calibration Curves of DCD and Melamine Overlapping of Six MRM Peaks of 0.5 ng/mL DCD and Melamine in Milk Powder Matrix

Analysis of Trace Element Contamination in a Fish ICP Atomic Emission Spectrometer ICPE-9800 Series Analysis of Preservatives in Food (UV) Analysis of Sudan Dyes in Curry Powder (LC) Sample (ICP) Below are examples of the analysis of food samples spiked with Sudan dyes are oil-soluble synthetic dyes used in industrial Fish Protein Certified Reference Material for Trace Metals preservatives. products. Their use is not authorized in food products in some (DORM-4) was used as the fish sample. The Shimadzu countries. This is an example of LC analysis of Sudan dyes in ICPE-9800 can provide a rapid method to simultaneously 0.7 8µg curry powder. analyze trace elements in fish. The results show excellent 0.6

6µg correlation with the certified reference material. 0.7 0.5 Peaks 0.5µg mAU 3 4 1 Sudan Orange G-a 0.6 0.4µg 0.4 2 Sudan Orange G-b Cd 214.438nm Standards Cr 205.552nm Standards 0.5 Abs. 4µg 3 Sudan Red G 0.3µg 20 5 0.4 6 Sample 0.3 4 Sudan I Abs. 0.3 5 Sudan II Samples Samples 0.2µg 0.2 2µg 15 6 Sudan III 0.2 2 8 7 Sudan Red 7B 0.1µg 0.1 7 Peak Profiles of Cd and Cr 0.1 8 Sudan IV 10 Curry powder 450 500 550 600 650 250 300 350 1 nm nm +Sudan dyes 5 Usage and Features of Elemental Analysis System Sodium Nitrite in Meat Sorbic Acid in a Processed White Fish Product Curry powder 0 Product Usage / Feature Sample 0.0 2.5 5.0 7.5 10.0 12.5 15.0 min Atomic absorption spectrophotometry is widely used for tests compliant with various standards Atomic Absorption UV-VIS Spectrophotometer UV-1900 Sudan Dyes in Curry Powder due to its high sensitivity. AA-7000 offers superior expandability due to its flexible system Liquid (or sample Spectrophotometer configuration. It also offers numerous safety features, such as a vibration sensor and a gas dissolved in solvent) AA-7000 Series Features Source: Shimadzu Analysis Guidebook No. C180-E059, page 77 leakage detection system, as standard.

ICP atomic emission spectrometry is widely used for tests compliant with various standards due to High Performance ICP Atomic Emission its high sensitivity and rapidity when performing multi-element simultaneous analyses. ICPE-9800 Liquid (or sample ・Ultra-fast scan speed (29,000nm/min) Spectrometer series offers numerous features, including the simultaneous analysis of all elements, simple dissolved in solvent) ICPE-9800 Series ・Low stray light (less than 0.5% at 198nm) operation with its assistant functions, and low running costs. ・High photometric repeatability (less than ICP mass spectrometry is a method of analysis that provides ultra-high sensitivity at ppt levels in ±0.0002Abs at 0.5Abs and 1.0Abs) ICP Mass Spectrometer addition to simultaneous multi-element analysis capabilities. ICPMS-2030, while being highly Liquid (or sample Intuitive Operability ICPMS-2030 sensitive, also offers low running costs by reducing the argon gas flow. It also greatly improves dissolved in solvent) the reliability of data through its assistant functions. Easy-to-navigate color touch-screen display Instrument Validation X-ray fluorescence spectrometry makes possible the nondestructive analysis, without X-Ray Fluorescence pretreatment, of the elements in solid, powder, or liquid samples. EDX-7000/8000 are capable of Automatic/semi-automatic validation of Spectrometer Solid, powder, liquid detecting sub-ppm level heavy elements. Offering low running costs and superior maintenance wavelength accuracy and wavelength EDX-7000/8000 features, these instruments are suitable for the screening of toxic metals. reproducibility.

Shimadzu’s Total Support for Food Safety 10 Analytical and Testing Instruments for Food Safety 11 Analysis of Toxic Metals Analysis of Food Additives

Toxic metals (arsenic, lead, cadmium, etc.) in foods must be controlled down to low concentration levels. A wide variety of additives, such as preservatives, flavor enhancers, coloring agents and antioxidants, are used on foods. Shimadzu atomic absorption spectrophotometers and ICP emission spectrometers offer highly sensitive analysis of trace Strict monitoring of food stuffs is essential to detect the use of prohibited chemicals. Each country assigns a unique number or elements in foods. Shimadzu’s product lineup also includes X-ray fluorescence spectrometers that can perform elemental code of approved chemicals for that country. Shimadzu offers a wide range of products for detecting the prohibited analysis without sample pretreatment. These instruments are ideal for screening analysis and emergency analysis. chemicals, whether they were added intentionally or through contamination.

Analysis of Cd and Pb in Food Additives (AA) Atomic Absorption Spectrophotometer AA-7000 Analysis of Dicyandiamide and Melamine in Milk Powders (LC-MS/MS)

Shown here is an analysis of cadmium (Cd) and lead (Pb) in Melamine was found to be used as a protein-rich adulterant residues were found in milk products from New Zealand. α-cyclodextrin (cyclic oligosaccharide), a substance used in in pet food in 2007, and then in infant formula in 2008 in Instead of being added directly as an adulterant, the trace functional foods, pharmaceuticals, cosmetics, etc. The China. The outbreak of the melamine scandal that killed DCD, which is used for promote the growth of cow pastures, analysis was conducted by the AA-7000 atomic absorption many dogs and cats as well as led to the deaths of six infants found in milk products was explained to be relating to the

Abs Abs and illness of many others caused panic and great concerns grass “contaminated by DCD”. Cd Pb worldwide. Recently, low levels of dicyandiamide (DCD) (x100) (x1,000) 4.5 STD 1 µg/L 6.0 Area (x10,000) Area (x100,000) DCD 4.0 Melamine STD 10 µg/L (85.1>68.1) (127.1>85.1) 3.5 5.0 3.5 7.5 DCD (85.1>68.1) Melamine (127.1>85.1) 3.0 R2 = 0.997 R2 = 0.999 4.0 3.0 Sample Sample 2.5 2.5 w/Spike w/Spike 5.0 3.0 2.0 2.0 Measurement Results of Cd and Pb in α-cyclodextrin 1.5 2.0 1.5 Sample Element Cd Pb 2.5 1.0 1.0 Sample w/o Spike 1.0 0.5 w/o Spike Measured value <0.003 µg/g <0.07 µg/g 0.5 0.0 0.0 Spike and recovery rate 105 % 99 % 0.0 0.0 0 3sec 0 3sec 0.0 2.5 5.0 7.5 Conc. 0.0 2.5 5.0 7.5 Conc. 2.00 2.25 2.50 2.75 min 5.5 6.0 6.5 min Peak Profiles of Cd and Pb Calibration Curves of DCD and Melamine Overlapping of Six MRM Peaks of 0.5 ng/mL DCD and Melamine in Milk Powder Matrix

Analysis of Trace Element Contamination in a Fish ICP Atomic Emission Spectrometer ICPE-9800 Series Analysis of Preservatives in Food (UV) Analysis of Sudan Dyes in Curry Powder (LC) Sample (ICP) Below are examples of the analysis of food samples spiked with Sudan dyes are oil-soluble synthetic dyes used in industrial Fish Protein Certified Reference Material for Trace Metals preservatives. products. Their use is not authorized in food products in some (DORM-4) was used as the fish sample. The Shimadzu countries. This is an example of LC analysis of Sudan dyes in ICPE-9800 can provide a rapid method to simultaneously 0.7 8µg curry powder. analyze trace elements in fish. The results show excellent 0.6

6µg correlation with the certified reference material. 0.7 0.5 Peaks 0.5µg mAU 3 4 1 Sudan Orange G-a 0.6 0.4µg 0.4 2 Sudan Orange G-b Cd 214.438nm Standards Cr 205.552nm Standards 0.5 Abs. 4µg 3 Sudan Red G 0.3µg 20 5 0.4 6 Sample 0.3 4 Sudan I Abs. 0.3 5 Sudan II Samples Samples 0.2µg 0.2 2µg 15 6 Sudan III 0.2 2 8 7 Sudan Red 7B 0.1µg 0.1 7 Peak Profiles of Cd and Cr 0.1 8 Sudan IV 10 Curry powder 450 500 550 600 650 250 300 350 1 nm nm +Sudan dyes 5 Usage and Features of Elemental Analysis System Sodium Nitrite in Meat Sorbic Acid in a Processed White Fish Product Curry powder 0 Product Usage / Feature Sample 0.0 2.5 5.0 7.5 10.0 12.5 15.0 min Atomic absorption spectrophotometry is widely used for tests compliant with various standards Atomic Absorption UV-VIS Spectrophotometer UV-1900 Sudan Dyes in Curry Powder due to its high sensitivity. AA-7000 offers superior expandability due to its flexible system Liquid (or sample Spectrophotometer configuration. It also offers numerous safety features, such as a vibration sensor and a gas dissolved in solvent) AA-7000 Series Features Source: Shimadzu Analysis Guidebook No. C180-E059, page 77 leakage detection system, as standard.

ICP atomic emission spectrometry is widely used for tests compliant with various standards due to High Performance ICP Atomic Emission its high sensitivity and rapidity when performing multi-element simultaneous analyses. ICPE-9800 Liquid (or sample ・Ultra-fast scan speed (29,000nm/min) Spectrometer series offers numerous features, including the simultaneous analysis of all elements, simple dissolved in solvent) ICPE-9800 Series ・Low stray light (less than 0.5% at 198nm) operation with its assistant functions, and low running costs. ・High photometric repeatability (less than ICP mass spectrometry is a method of analysis that provides ultra-high sensitivity at ppt levels in ±0.0002Abs at 0.5Abs and 1.0Abs) ICP Mass Spectrometer addition to simultaneous multi-element analysis capabilities. ICPMS-2030, while being highly Liquid (or sample Intuitive Operability ICPMS-2030 sensitive, also offers low running costs by reducing the argon gas flow. It also greatly improves dissolved in solvent) the reliability of data through its assistant functions. Easy-to-navigate color touch-screen display Instrument Validation X-ray fluorescence spectrometry makes possible the nondestructive analysis, without X-Ray Fluorescence pretreatment, of the elements in solid, powder, or liquid samples. EDX-7000/8000 are capable of Automatic/semi-automatic validation of Spectrometer Solid, powder, liquid detecting sub-ppm level heavy elements. Offering low running costs and superior maintenance wavelength accuracy and wavelength EDX-7000/8000 features, these instruments are suitable for the screening of toxic metals. reproducibility.

Shimadzu’s Total Support for Food Safety 10 Analytical and Testing Instruments for Food Safety 11 Analysis of Food Additives Food Processing

Since food additives are widely used in food products, simultaneous analysis is essential to confirm proper use of them. Food processing takes a farmed product and turns it in to an edible, marketable product that may have a longer shelf life. Efficient methods using GC-MS and LC-MS have been established for the simultaneous determination of several food additives Examples of these processes are baking, canning, fermenting, grilling, heating, hydrolysis, and roasting. During these like flavor enhancers and sweeteners. With these simultaneous analysis methods, multiple compounds can be efficiently processes, undesirable and even toxic compounds can form. Or cross-contamination can occur from the big eight allergens of analyzed in short time. egg, fish, milk, peanuts, shellfish, soy, tree nuts, and wheat.

Analysis of Acrylamide in Potato Chips (LC-MS/MS) Single Quadrupole Mass Spectrometer Simultaneous Analysis of Flavor Compounds (GC-MS) GCMS-QP2020 Acrylamide was found to form in fried foods like potato chips via the so-called Maillard reaction of amino acid asparagine GC-MS is commonly used for analysis of flavor compounds. and reducing sugar glucose at a higher temperature (120°C). The health risk of acrylamide present in many processed foods Shown is an example of a simultaneous analysis of 19 flavor was an immediate concern, because it is known that the compound is a neurotoxin and a potential carcinogen to humans. compounds in lavender oil using a Fast GC/MS Method. All compounds were separated within six minutes. Y= 1.1239X + 0.594168 R2 = 0.9999

(x1,000,000) 9 7

TIC 10

3.0 13 1

2.5 3

2.0 6

1.5 12 2

1.0 5 11 15 14 0.5 4 8 16 19 17 18 Calibration Curve (left) and MRM Peaks (right) of Acrylamide Spiked into Potato Chips Matrix, 1-500 ppb with 50 ppb IS (internal standard) Added. 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 min Peaks Source: ASMS 2014 Poster No. MP342 1 alpha-Pinene 6 Limonene 11 Isoborneol 16 (Z)-beta-Farnesene 2 Camphene 7 Eucalyptol 12 alpha-Terpineol 17 Germacrene D 3 beta-Pinene 8 gamma-Terpinene 13 Linalyl acetate 18 beta-Bisabolene 4 Myrcene 9 Linalool 14 Bornyl acetate 19 Caryophyllene oxide 5 Cymene 10 Camphor 15 Neryl acetate Source: Shimadzu Application Data Sheet GCMS No. 32

Triple Quadrupole Mass Spectrometer Simultaneous Analysis of Sweeteners (LC-MS/MS) LCMS-8060 A wide range of low-calorie sweeteners are often used in foods. They are used together with sugars, and multiple sweeteners are combined to regulate flavors. This example shows the simultaneous analysis of 16 sweeteners. Good linearity was obtained with a correlation coefficient of 0.997 or higher for all the compounds.

Calibration Curve Correlation Compound Name Polarity Transition Holding Time (min) Range (ng/mL) Sucralose + 414.00>199.10 6.36 0.5 ‐ 100 0.999 Dulcin + 181.20>108.10 6.70 0.05 ‐ 10 0.999 Alitame + 332.20>129.00 6.92 0.5 ‐ 100 0.999 Rebaudioside A + 984.50>325.10 8.21 0.5 ‐ 100 0.999 Stevioside + 822.00>319.30 8.23 0.5 ‐ 100 0.999 Acesulfame potassium - 161.90>82.00 5.23 0.1 ‐ 10 0.999 Saccharin - 181.90>42.00 5.58 0.5 ‐ 50 0.997 Cyclamate - 178.00>80.00 6.08 1 ‐ 100 0.999 Aspartame - 293.40>261.10 6.53 5 ‐ 100 0.999 Advantame - 457.30>200.30 7.12 0.5 ‐ 100 0.999 Glycyrrhizic acid - 821.20>351.10 7.41 50 ‐ 1000 0.999 Rebaudioside M - 1289.60>802.90 7.66 50 ‐ 1000 0.999 Neotame - 377.30>200.00 7.90 1 ‐ 100 0.999 Rebaudioside C - 949.50>787.20 8.46 1 ‐ 100 0.999 Dulcoside A - 787.50>625.20 8.50 10 ‐ 1000 0.999 Isosteviol - 317.30>317.30 10.46 0.5 ‐ 1000 0.999 Source: Shimadzu Application News No. C133.

Shimadzu’s Total Support for Food Safety 12 Analytical and Testing Instruments for Food Safety 13 Analysis of Food Additives Food Processing

Since food additives are widely used in food products, simultaneous analysis is essential to confirm proper use of them. Food processing takes a farmed product and turns it in to an edible, marketable product that may have a longer shelf life. Efficient methods using GC-MS and LC-MS have been established for the simultaneous determination of several food additives Examples of these processes are baking, canning, fermenting, grilling, heating, hydrolysis, and roasting. During these like flavor enhancers and sweeteners. With these simultaneous analysis methods, multiple compounds can be efficiently processes, undesirable and even toxic compounds can form. Or cross-contamination can occur from the big eight allergens of analyzed in short time. egg, fish, milk, peanuts, shellfish, soy, tree nuts, and wheat.

Analysis of Acrylamide in Potato Chips (LC-MS/MS) Single Quadrupole Mass Spectrometer Simultaneous Analysis of Flavor Compounds (GC-MS) GCMS-QP2020 Acrylamide was found to form in fried foods like potato chips via the so-called Maillard reaction of amino acid asparagine GC-MS is commonly used for analysis of flavor compounds. and reducing sugar glucose at a higher temperature (120°C). The health risk of acrylamide present in many processed foods Shown is an example of a simultaneous analysis of 19 flavor was an immediate concern, because it is known that the compound is a neurotoxin and a potential carcinogen to humans. compounds in lavender oil using a Fast GC/MS Method. All compounds were separated within six minutes. Y= 1.1239X + 0.594168 R2 = 0.9999

(x1,000,000) 9 7

TIC 10

3.0 13 1

2.5 3

2.0 6

1.5 12 2

1.0 5 11 15 14 0.5 4 8 16 19 17 18 Calibration Curve (left) and MRM Peaks (right) of Acrylamide Spiked into Potato Chips Matrix, 1-500 ppb with 50 ppb IS (internal standard) Added. 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 min Peaks Source: ASMS 2014 Poster No. MP342 1 alpha-Pinene 6 Limonene 11 Isoborneol 16 (Z)-beta-Farnesene 2 Camphene 7 Eucalyptol 12 alpha-Terpineol 17 Germacrene D 3 beta-Pinene 8 gamma-Terpinene 13 Linalyl acetate 18 beta-Bisabolene 4 Myrcene 9 Linalool 14 Bornyl acetate 19 Caryophyllene oxide 5 Cymene 10 Camphor 15 Neryl acetate Source: Shimadzu Application Data Sheet GCMS No. 32

Triple Quadrupole Mass Spectrometer Simultaneous Analysis of Sweeteners (LC-MS/MS) LCMS-8060 A wide range of low-calorie sweeteners are often used in foods. They are used together with sugars, and multiple sweeteners are combined to regulate flavors. This example shows the simultaneous analysis of 16 sweeteners. Good linearity was obtained with a correlation coefficient of 0.997 or higher for all the compounds.

Calibration Curve Correlation Compound Name Polarity Transition Holding Time (min) Range (ng/mL) Sucralose + 414.00>199.10 6.36 0.5 ‐ 100 0.999 Dulcin + 181.20>108.10 6.70 0.05 ‐ 10 0.999 Alitame + 332.20>129.00 6.92 0.5 ‐ 100 0.999 Rebaudioside A + 984.50>325.10 8.21 0.5 ‐ 100 0.999 Stevioside + 822.00>319.30 8.23 0.5 ‐ 100 0.999 Acesulfame potassium - 161.90>82.00 5.23 0.1 ‐ 10 0.999 Saccharin - 181.90>42.00 5.58 0.5 ‐ 50 0.997 Cyclamate - 178.00>80.00 6.08 1 ‐ 100 0.999 Aspartame - 293.40>261.10 6.53 5 ‐ 100 0.999 Advantame - 457.30>200.30 7.12 0.5 ‐ 100 0.999 Glycyrrhizic acid - 821.20>351.10 7.41 50 ‐ 1000 0.999 Rebaudioside M - 1289.60>802.90 7.66 50 ‐ 1000 0.999 Neotame - 377.30>200.00 7.90 1 ‐ 100 0.999 Rebaudioside C - 949.50>787.20 8.46 1 ‐ 100 0.999 Dulcoside A - 787.50>625.20 8.50 10 ‐ 1000 0.999 Isosteviol - 317.30>317.30 10.46 0.5 ‐ 1000 0.999 Source: Shimadzu Application News No. C133.

Shimadzu’s Total Support for Food Safety 12 Analytical and Testing Instruments for Food Safety 13 Analysis of Allergens Analysis of Off-Flavor

Food allergies are a major concern due to their adverse health effects. To avoid unexpected contact with food allergens, many If unforeseen circumstances occur during food product manufacture, storage, transport or other process and an off-flavor countries stipulate the application of food labels that indicate the presence of specific allergens. An example of the issue occurs, it is necessary to specify the odor-causing substances in order to resolve the problem and prevent a recurrence. simultaneous analysis of allergens using LC-MS/MS is shown; it contains 150 MRMs for 31 peptides of 13 allergenic proteins Shimadzu provides a total solution necessary for analyzing odors combining GC-MS and a database for off-flavor analysis, so identified from 8 foods regulated in the US (milk, eggs, fish, crustacean shellfish, tree nuts, peanuts, wheat and soybeans). even analysts without knowledge or experience of off-flavor analysis can start analysis. They were successfully detected with high sensitivity and selectivity.

Off-Flavor Analyzer Triple Quadrupole Mass Spectrometer Simultaneous Analysis of Food Allergens (LC-MS/MS) GCMS-8050 + Database for Off-Flavor Analysis LCMS-8060 Chromatogram of a peptides mixture derived from eight Features food allergens, and a magnified view of five MRM transitions for wheat peptides and its calibration curve. Database of Expert Information for Odor Analysis Database containing odor-causing substances identified from

Area 8000 7500 SVAVSQVAR (1 - 50 ppm) previous problems and associated sensory information supports odor 7000 Y=151.944x + 231.865 6500 6000 R2 > 0.999188 5500 5000 analysis. 4500 4000 3500 3000 2500 2000 Analytical System for Reliably Identifying Odor-causing Substances 1500 1000 500 0 0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 Conc. Optimized sample pretreatments and analytical conditions including three types of columns, SIM/MRM conditions and retention time Containing Experts’ Knowhow for Odor Analysis enable effective identification of odor-causing substances.

What is the Off-Flavor Analyzer? This system combines a database of the major odor-causing

substances and associated sensory information (odor characteristics Source: Shimadzu Application News No. C160 and threshold levels for sensing odors) with GC-MS. Even analysts Odor Components Analytical Conditions Sensory Information of Odor Components without knowledge or experience of off-flavor analysis can start an

Chromatogram of Different Bread Types analysis easily.

Analysis of Food Product Samples Involved in Off-Flavor Complaints

In the analysis of food product samples involved in off-flavor complaints, it was confirmed that four components are more often found in off-flavor products than in normal products. The four components detected were further analyzed using the off-flavor analysis database, and concentration and odor threshold values as well as odor characteristics were confirmed. From the results, 2,4,6-trichloroanisole was identified as an odor-causing substance.

146.0>111.0 129.0>87.0 161.0>133.0 195.0>167.0 146.0>75.0 Odor 115.0>69.0 Odor 4.0 176.0>161.0 Odor 1.25 210.0>195.0 Odor 111.0>75.0 1.5 115.0>45.0 176.0>133.0 210.0>167.0 5.0 1.00 3.0 1.0 0.75 2.0 2.5 0.50 0.5 Ordinary Bread Gluten Free Bread Gluten Free Bread 1.0 0.25 Spiked with 10 ppm of Wheat 0.0 0.0 0.0 0.00 12.0 12.5 16.5 17.0 17.0 17.5 17.5 18.0

146.0>111.0 129.0>87.0 161.0>133.0 195.0>167.0 146.0>75.0 Normal 115.0>69.0 Normal 4.0 176.0>161.0 Normal 1.25 210.0>195.0 Normal Analytical Results of Allergens in Food Samples 111.0>75.0 1.5 176.0>133.0 210.0>167.0 5.0 115.0>45.0 3.0 1.00 Gluten Free Peanut Frozen Fish Frozen Pasta Bread 1.0 0.75 Food Allergens Bread Cookies “Fried Cod” “Garlic Shrimps” 2.0 2.5 0.50 Label Detect Label Detect Label Detect Label Detect Label Detect 0.5 1.0 0.25 Bos d 8 Milk - 0.0 0.0 0.0 0.00 Bos d 5 12.0 12.5 16.5 17.0 17.0 17.5 17.5 18.0 Gal d 2 - Egg Gal d 3 p-dichlorobenzene pelargonic acid 2,4-dichloroanisole 2,4,6-trichloroanisole Atlantic Cod Gad m 1 - Four Higher Concentration Compounds Estimated Concentration (pg/mg) Odor Threshold Values Lit v 1 Odor Characteristics Whiteleg Shrimp Lit v 3 - in Odor Sample Normal Sample Odor Sample (pg/mg) Lit v 4 p-dichlorobenzene 0.052 66.558 1000.000 Insecticide Almonds Pru du 6 - pelargonic acid 0 0.851 100.000 Acid like dried fruits Peanuts Ara h 1 - 2,4-dichloroanisole 0 0.003 10.000 Mold Wheat Tri a 26 - Tri a 36 2,4,6-trichloroanisole 0 0.009 0.001 Mold - Soybeans Gly m TI Source: Shimadzu Technical Report No. C146-E293

Shimadzu’s Total Support for Food Safety 14 Analytical and Testing Instruments for Food Safety 15 Analysis of Allergens Analysis of Off-Flavor

Food allergies are a major concern due to their adverse health effects. To avoid unexpected contact with food allergens, many If unforeseen circumstances occur during food product manufacture, storage, transport or other process and an off-flavor countries stipulate the application of food labels that indicate the presence of specific allergens. An example of the issue occurs, it is necessary to specify the odor-causing substances in order to resolve the problem and prevent a recurrence. simultaneous analysis of allergens using LC-MS/MS is shown; it contains 150 MRMs for 31 peptides of 13 allergenic proteins Shimadzu provides a total solution necessary for analyzing odors combining GC-MS and a database for off-flavor analysis, so identified from 8 foods regulated in the US (milk, eggs, fish, crustacean shellfish, tree nuts, peanuts, wheat and soybeans). even analysts without knowledge or experience of off-flavor analysis can start analysis. They were successfully detected with high sensitivity and selectivity.

Off-Flavor Analyzer Triple Quadrupole Mass Spectrometer Simultaneous Analysis of Food Allergens (LC-MS/MS) GCMS-8050 + Database for Off-Flavor Analysis LCMS-8060 Chromatogram of a peptides mixture derived from eight Features food allergens, and a magnified view of five MRM transitions for wheat peptides and its calibration curve. Database of Expert Information for Odor Analysis Database containing odor-causing substances identified from

Area 8000 7500 SVAVSQVAR (1 - 50 ppm) previous problems and associated sensory information supports odor 7000 Y=151.944x + 231.865 6500 6000 R2 > 0.999188 5500 5000 analysis. 4500 4000 3500 3000 2500 2000 Analytical System for Reliably Identifying Odor-causing Substances 1500 1000 500 0 0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 Conc. Optimized sample pretreatments and analytical conditions including three types of columns, SIM/MRM conditions and retention time Containing Experts’ Knowhow for Odor Analysis enable effective identification of odor-causing substances.

What is the Off-Flavor Analyzer? This system combines a database of the major odor-causing

substances and associated sensory information (odor characteristics Source: Shimadzu Application News No. C160 and threshold levels for sensing odors) with GC-MS. Even analysts Odor Components Analytical Conditions Sensory Information of Odor Components without knowledge or experience of off-flavor analysis can start an

Chromatogram of Different Bread Types analysis easily.

Analysis of Food Product Samples Involved in Off-Flavor Complaints

In the analysis of food product samples involved in off-flavor complaints, it was confirmed that four components are more often found in off-flavor products than in normal products. The four components detected were further analyzed using the off-flavor analysis database, and concentration and odor threshold values as well as odor characteristics were confirmed. From the results, 2,4,6-trichloroanisole was identified as an odor-causing substance.

146.0>111.0 129.0>87.0 161.0>133.0 195.0>167.0 146.0>75.0 Odor 115.0>69.0 Odor 4.0 176.0>161.0 Odor 1.25 210.0>195.0 Odor 111.0>75.0 1.5 115.0>45.0 176.0>133.0 210.0>167.0 5.0 1.00 3.0 1.0 0.75 2.0 2.5 0.50 0.5 Ordinary Bread Gluten Free Bread Gluten Free Bread 1.0 0.25 Spiked with 10 ppm of Wheat 0.0 0.0 0.0 0.00 12.0 12.5 16.5 17.0 17.0 17.5 17.5 18.0

146.0>111.0 129.0>87.0 161.0>133.0 195.0>167.0 146.0>75.0 Normal 115.0>69.0 Normal 4.0 176.0>161.0 Normal 1.25 210.0>195.0 Normal Analytical Results of Allergens in Food Samples 111.0>75.0 1.5 176.0>133.0 210.0>167.0 5.0 115.0>45.0 3.0 1.00 Gluten Free Peanut Frozen Fish Frozen Pasta Bread 1.0 0.75 Food Allergens Bread Cookies “Fried Cod” “Garlic Shrimps” 2.0 2.5 0.50 Label Detect Label Detect Label Detect Label Detect Label Detect 0.5 1.0 0.25 Bos d 8 Milk - 0.0 0.0 0.0 0.00 Bos d 5 12.0 12.5 16.5 17.0 17.0 17.5 17.5 18.0 Gal d 2 - Egg Gal d 3 p-dichlorobenzene pelargonic acid 2,4-dichloroanisole 2,4,6-trichloroanisole Atlantic Cod Gad m 1 - Four Higher Concentration Compounds Estimated Concentration (pg/mg) Odor Threshold Values Lit v 1 Odor Characteristics Whiteleg Shrimp Lit v 3 - in Odor Sample Normal Sample Odor Sample (pg/mg) Lit v 4 p-dichlorobenzene 0.052 66.558 1000.000 Insecticide Almonds Pru du 6 - pelargonic acid 0 0.851 100.000 Acid like dried fruits Peanuts Ara h 1 - 2,4-dichloroanisole 0 0.003 10.000 Mold Wheat Tri a 26 - Tri a 36 2,4,6-trichloroanisole 0 0.009 0.001 Mold - Soybeans Gly m TI Source: Shimadzu Technical Report No. C146-E293

Shimadzu’s Total Support for Food Safety 14 Analytical and Testing Instruments for Food Safety 15 Inspection and Analysis of Foreign Matter Prevention of Defects in Packaging

Foreign matter can become mixed with food products during the production process for a variety of reasons. The discovery Testing the strength and functionality of packaging materials is essential to ensure food products reach consumers fresh and and identification of foreign matter and ascertaining its source are essential for maintaining food safety. undamaged. To aid the development and quality control testing of packaging materials, Shimadzu analytical and testing Shimadzu’s non-destructive testing and analytical technologies can be used to detect foreign matter that is not externally technologies allow the analysis of residual solvents in packaging materials, strength testing of adhesive seals, and checking of visible and analyze for the elemental or molecular components. pin holes in packaged products.

Adhesive Strength Testing (Strength Tester) EZ Test Universal Testing Machines Analysis of Inorganic Foreign Matter (EDX) Energy Dispersive X-ray Fluorescence Spectrometer EDX-7000/8000/8100 A wide range of materials, including aluminum and other An energy-dispersive X-ray fluorescence (EDXRF) spectrometer metals and non-metallic materials such as paper and plastic, permits the easy and rapid identification of inorganic foreign matter is used for packaging. According to their application, these adhering to, or mixed into, food products. Below is an example of materials must meet certain tensile, compression, and the qualitative analysis of a metal fragment on chocolate. bending strength requirements, and if an adhesive is used, adhesive strength requirements. In the examples below,

Quantitative adhesive strength results for liquid-filled pouch and dry analysis results Features snack package specimens are shown. In both instances, Fe 70.06% Cr 19.18% Large Sample Chamber the adhesives meet the requirements of being strong enough Ni 9.77% Mo 0.51% Accepts samples of up W300 x D275 x H100 mm. to protect the contents, but weak enough so that the Cu 0.48% Easy Operation contents can easily be accessed by consumers.

N15 Automates complex set-up operations. Even novices can easily N40 1-1 1-1 1-2 12 1-2 conduct accurate measurements. 30 1-3 1-3 Metal Foreign Matter on Chocolate 9 Features 20 6

10 3 Comprehensive Range of Jigs

IRTracer-100 FTIR Spectrophotometer 0 0 Diverse strength testing: peeling, tensile, shear, bending, Analysis of Organic Foreign Matter (FTIR) 0 5 10 15 20 0 7 14 21 30 min min compression, etc. The Fourier transform infrared (FTIR) spectrophotometer measures Liquid-filled Pouch Dry Snack Packaging the organic foreign matter spectrum and compares it with library Measures Brittle and Elastic Samples 5 kN max. capacity, 920 mm stroke (long-type). data to identify the foreign matter. The example below shows the analysis of contamination on a frozen pizza. Some of the foreign Analysis of MOSH/MOAH (LC-GC Online System) Food Texture Evaluation Objective testing which analyzes the consistency and mouth feel of substance was scraped off the frozen pizza, and the infrared Analysis of MOSH/MOAH (LC-GC Online System) foods. spectrum was measured using a transmission infrared microscope. The There‘s concern about the influence of mineral oil components such as spectrum of this foreign object was similar to that of a fluororesin. Features MOSH (mineral oil saturated hydrocarbons) and MOAH (mineral oil aromatic hydrocarbons) on the human body. Food can get contaminated High Sensitivity 1 with MOSH/MOAH through contacts with packaging materials and printing 1000000 High S/N ratio above 60,000:1. Microscope permits analysis of colors of packaging. LC-GC online system with autosampler enables Abs foreign matter in the micron size range. 750000 effective and high throughput analysis of MOSH and MOAH in food and contaminant Foreign Matter Analysis Program as Standard 0.5 food packaging. After separation in HPLC, MOSH and MOAH fractions are Powerful support for foreign matter analysis. Includes more than 500000 automatically transferred to GC and detected by two FID simultaneously. 300 spectra of recognized foreign matter. It's a powerful solution for the analysis of non-intentionally added fluororesin Easy Maintenance 250000 -0 substances (NIAS) such as mineral oil hydrocarbons. 4000 3000 2000 1500 1000 1/cm Automatic dryer protects the optical system. Eliminates superfluous

0 maintenance. LC-GC Online System with Autosampler for MOSH/MOAH Analysis* 0 2,5 5,0 7,5 10,0 12,5 15,0 17,5 20,0 22,5 min Infrared Spectra of Contaminant and Fluororesin Nexera X2 + Nexis GC-2030 + PAL Autosampler uV 1000000

EDXIR-Analysis software is specially designed to perform an Software for Integrated Analysis of EDX and FTIR Data 750000 integrated analysis of data from FTIR and EDX. The (EDXIR-Analysis)

library used for data analysis (containing 485 data as 500000 standard) is original to Shimadzu.

250000 EDX Data

0 0 2,5 5,0 7,5 10,0 12,5 15,0 17,5 20,0 22,5 min

Chromatogram of MOSH/MOAH in Food and Food Packaging

FTIR Data *Configuration may not be available in all countries. Check with your Shimadzu Integrated Data Analysis of Foreign Matter representative. Source: AOAC 2017 Poster No. P-W-021

Shimadzu’s Total Support for Food Safety 16 Analytical and Testing Instruments for Food Safety 17 Inspection and Analysis of Foreign Matter Prevention of Defects in Packaging

Foreign matter can become mixed with food products during the production process for a variety of reasons. The discovery Testing the strength and functionality of packaging materials is essential to ensure food products reach consumers fresh and and identification of foreign matter and ascertaining its source are essential for maintaining food safety. undamaged. To aid the development and quality control testing of packaging materials, Shimadzu analytical and testing Shimadzu’s non-destructive testing and analytical technologies can be used to detect foreign matter that is not externally technologies allow the analysis of residual solvents in packaging materials, strength testing of adhesive seals, and checking of visible and analyze for the elemental or molecular components. pin holes in packaged products.

Adhesive Strength Testing (Strength Tester) EZ Test Universal Testing Machines Analysis of Inorganic Foreign Matter (EDX) Energy Dispersive X-ray Fluorescence Spectrometer EDX-7000/8000/8100 A wide range of materials, including aluminum and other An energy-dispersive X-ray fluorescence (EDXRF) spectrometer metals and non-metallic materials such as paper and plastic, permits the easy and rapid identification of inorganic foreign matter is used for packaging. According to their application, these adhering to, or mixed into, food products. Below is an example of materials must meet certain tensile, compression, and the qualitative analysis of a metal fragment on chocolate. bending strength requirements, and if an adhesive is used, adhesive strength requirements. In the examples below,

Quantitative adhesive strength results for liquid-filled pouch and dry analysis results Features snack package specimens are shown. In both instances, Fe 70.06% Cr 19.18% Large Sample Chamber the adhesives meet the requirements of being strong enough Ni 9.77% Mo 0.51% Accepts samples of up W300 x D275 x H100 mm. to protect the contents, but weak enough so that the Cu 0.48% Easy Operation contents can easily be accessed by consumers.

N15 Automates complex set-up operations. Even novices can easily N40 1-1 1-1 1-2 12 1-2 conduct accurate measurements. 30 1-3 1-3 Metal Foreign Matter on Chocolate 9 Features 20 6

10 3 Comprehensive Range of Jigs

IRTracer-100 FTIR Spectrophotometer 0 0 Diverse strength testing: peeling, tensile, shear, bending, Analysis of Organic Foreign Matter (FTIR) 0 5 10 15 20 0 7 14 21 30 min min compression, etc. The Fourier transform infrared (FTIR) spectrophotometer measures Liquid-filled Pouch Dry Snack Packaging the organic foreign matter spectrum and compares it with library Measures Brittle and Elastic Samples 5 kN max. capacity, 920 mm stroke (long-type). data to identify the foreign matter. The example below shows the analysis of contamination on a frozen pizza. Some of the foreign Analysis of MOSH/MOAH (LC-GC Online System) Food Texture Evaluation Objective testing which analyzes the consistency and mouth feel of substance was scraped off the frozen pizza, and the infrared Analysis of MOSH/MOAH (LC-GC Online System) foods. spectrum was measured using a transmission infrared microscope. The There‘s concern about the influence of mineral oil components such as spectrum of this foreign object was similar to that of a fluororesin. Features MOSH (mineral oil saturated hydrocarbons) and MOAH (mineral oil aromatic hydrocarbons) on the human body. Food can get contaminated High Sensitivity 1 with MOSH/MOAH through contacts with packaging materials and printing 1000000 High S/N ratio above 60,000:1. Microscope permits analysis of colors of packaging. LC-GC online system with autosampler enables Abs foreign matter in the micron size range. 750000 effective and high throughput analysis of MOSH and MOAH in food and contaminant Foreign Matter Analysis Program as Standard 0.5 food packaging. After separation in HPLC, MOSH and MOAH fractions are Powerful support for foreign matter analysis. Includes more than 500000 automatically transferred to GC and detected by two FID simultaneously. 300 spectra of recognized foreign matter. It's a powerful solution for the analysis of non-intentionally added fluororesin Easy Maintenance 250000 -0 substances (NIAS) such as mineral oil hydrocarbons. 4000 3000 2000 1500 1000 1/cm Automatic dryer protects the optical system. Eliminates superfluous

0 maintenance. LC-GC Online System with Autosampler for MOSH/MOAH Analysis* 0 2,5 5,0 7,5 10,0 12,5 15,0 17,5 20,0 22,5 min Infrared Spectra of Contaminant and Fluororesin Nexera X2 + Nexis GC-2030 + PAL Autosampler uV 1000000

EDXIR-Analysis software is specially designed to perform an Software for Integrated Analysis of EDX and FTIR Data 750000 integrated analysis of data from FTIR and EDX. The (EDXIR-Analysis) library used for data analysis (containing 485 data as 500000 standard) is original to Shimadzu.

250000 EDX Data

0 0 2,5 5,0 7,5 10,0 12,5 15,0 17,5 20,0 22,5 min

Chromatogram of MOSH/MOAH in Food and Food Packaging

FTIR Data *Configuration may not be available in all countries. Check with your Shimadzu Integrated Data Analysis of Foreign Matter representative. Source: AOAC 2017 Poster No. P-W-021

Shimadzu’s Total Support for Food Safety 16 Analytical and Testing Instruments for Food Safety 17 Halal Testing Laboratory Data Management

The unauthorized blending of undeclared materials into food products has become a major concern among consumers. The increased demand for food safety analysis and measurement has led to a dramatic increase in the number of samples Such action violates customer rights based upon economical and safety values and it is also a critical problem for communities being analyzed which, in turn, has resulted in demands for faster, easier-to-use instruments and software. With this with ethical and religious beliefs. Shown below are the results from an analysis using a high-sensitivity LC-MS/MS method for background, faster, more efficient management of instruments and data is essential. LabSolutions is a network-compatible ensuring halal food integrity by targeting more heat-stable porcine-specific peptide markers in processed food. analysis data system capable of meeting these needs.

LabSolutions CS – Total Laboratory Network

Peptide Spiking Percentage of Cooked Pork (%, wt) [n=4] Marker Freely Accessible to the Analysis Network Recommended for the following customers 0.1 0.5 1.0 5.0 YDII + + + + Since all analytical data is managed in the database of a ・Facilities with a large number of instruments and users LVVI + + + + server computer, LabSolutions CS can read data from any ・Facilities interested in enhancing procedural efficiency personal computer on a network. In addition, analysis Facilities interested in enhancing managerial efficiency EVTE ND ND ND + ・ directions and instrument monitoring and control can be ・Facilities where existing PCs can be used as client PCs TVLG ND ND ND ND performed from a personal computer (client PC) not FVIE ND + + + connected to the instruments. TLAF ND ND + + Moreover, client PC functions are performed on a server and

SALA + + + + client PCs corresponding to a Windows terminal service do not need to install LabSolutions software.

Peptide LabSolutions server Candidate FASTA format MRM Prediction Method Development Client PC Selection download via Analysis equipment via Skyline & Analysis UniProt Database room or an office

(x1,000) (x1,000) (x1,000) Area(x10,000) Area(x100,000) 453.80>628.40(+) 450.30>687.40(+) 376.10>392.80(+) 1.00 453.80>279.MRM1 10(+) Pork 450.30>588.MRM1 35(+) 376.10>647.MRM1 20(+) Pork 453.80>515.MRM2 35(+) 450.30>475.MRM2 25(+) Pork 1.5 376.10>576.MRM2 40(+) 7.5 1.5 MRM3 MRM3 LVVI MRM3 YDII

0.1% SA LA 0.1% (A) 1.5 0.1% (B) 0.75 YDII LVVI 1.0 SALA 5.0 1.0 0.50 1.0

0.5 2.5 0.5 0.25 0.5

0.00 0.0 0.0 0.0 0.0 Acquisition 0.0 2.5 Conc. 0.0 2.5 Conc. UV FTIR RF ICPMS 6.0 6.5 7.0 5.0 5.5 6.0 3.0 3.5 controller PC (x1,000) (x10,000) 453.80>628.40(+) (x1,000) Area(x10,000) Area(x10,000) MRM1 450.30>687.40(+)MRM1 376.10>392.80(+) 7.5 453.80>279.10(+) 450.30>588.35(+) MRM1 453.MRM280>515.35(+) Pork MRM2 Pork 376.MRM210>647.20(+) Pork MRM3 1.25 450.30>475.25(+)MRM3 376.10>576.40(+) 4.0 YDII 3.0 MRM3 1.5 1.0% LVVI 1.0% 1.0% (C) (D) YDII 1.00 LVVI SA LA 3.0 5.0 2.0 SALA 1.0 0.75 GCMS AA TOC 2.0 0.50 2.5 1.0 0.5 1.0 0.25 EDX Particle size analyzer 0.0 0.00 0.0 0.0 0.0 0.0 2.5 Conc. 0.0 2.5 Conc. 6.0 7.0 5.0 5.5 6.0 3.0 4.0 Combine with a Multi Data Registration license to enable LC GC LC GC integrated management of these instruments’ data. Source: Shimadzu Application News No. AD-0153

In summary, the established MRM-based method on a LC-MS/MS platform offers a highly sensitive and selective approach to detect and monitor the presence of porcine-specific markers for semi-quantitation of the amount of pork in processed foods. The established method from sample preparation to LC-MS/MS analysis exhibits excellent reliable sensitivity, and is potentially able to support halal testing in processed food products. Analysis of various food matrices will be performed in the future.

Shimadzu’s Total Support for Food Safety 18 Analytical and Testing Instruments for Food Safety 19 Halal Testing Laboratory Data Management

The unauthorized blending of undeclared materials into food products has become a major concern among consumers. The increased demand for food safety analysis and measurement has led to a dramatic increase in the number of samples Such action violates customer rights based upon economical and safety values and it is also a critical problem for communities being analyzed which, in turn, has resulted in demands for faster, easier-to-use instruments and software. With this with ethical and religious beliefs. Shown below are the results from an analysis using a high-sensitivity LC-MS/MS method for background, faster, more efficient management of instruments and data is essential. LabSolutions is a network-compatible ensuring halal food integrity by targeting more heat-stable porcine-specific peptide markers in processed food. analysis data system capable of meeting these needs.

LabSolutions CS – Total Laboratory Network

Peptide Spiking Percentage of Cooked Pork (%, wt) [n=4] Marker Freely Accessible to the Analysis Network Recommended for the following customers 0.1 0.5 1.0 5.0 YDII + + + + Since all analytical data is managed in the database of a ・Facilities with a large number of instruments and users LVVI + + + + server computer, LabSolutions CS can read data from any ・Facilities interested in enhancing procedural efficiency personal computer on a network. In addition, analysis Facilities interested in enhancing managerial efficiency EVTE ND ND ND + ・ directions and instrument monitoring and control can be ・Facilities where existing PCs can be used as client PCs TVLG ND ND ND ND performed from a personal computer (client PC) not FVIE ND + + + connected to the instruments. TLAF ND ND + + Moreover, client PC functions are performed on a server and

SALA + + + + client PCs corresponding to a Windows terminal service do not need to install LabSolutions software.

Peptide LabSolutions server Candidate FASTA format MRM Prediction Method Development Client PC Selection download via Analysis equipment via Skyline & Analysis UniProt Database room or an office

(x1,000) (x1,000) (x1,000) Area(x10,000) Area(x100,000) 453.80>628.40(+) 450.30>687.40(+) 376.10>392.80(+) 1.00 453.80>279.MRM1 10(+) Pork 450.30>588.MRM1 35(+) 376.10>647.MRM1 20(+) Pork 453.80>515.MRM2 35(+) 450.30>475.MRM2 25(+) Pork 1.5 376.10>576.MRM2 40(+) 7.5 1.5 MRM3 MRM3 LVVI MRM3 YDII

0.1% SA LA 0.1% (A) 1.5 0.1% (B) 0.75 YDII LVVI 1.0 SALA 5.0 1.0 0.50 1.0

0.5 2.5 0.5 0.25 0.5

0.00 0.0 0.0 0.0 0.0 Acquisition 0.0 2.5 Conc. 0.0 2.5 Conc. UV FTIR RF ICPMS 6.0 6.5 7.0 5.0 5.5 6.0 3.0 3.5 controller PC (x1,000) (x10,000) 453.80>628.40(+) (x1,000) Area(x10,000) Area(x10,000) MRM1 450.30>687.40(+)MRM1 376.10>392.80(+) 7.5 453.80>279.10(+) 450.30>588.35(+) MRM1 453.MRM280>515.35(+) Pork MRM2 Pork 376.MRM210>647.20(+) Pork MRM3 1.25 450.30>475.25(+)MRM3 376.10>576.40(+) 4.0 YDII 3.0 MRM3 1.5 1.0% LVVI 1.0% 1.0% (C) (D) YDII 1.00 LVVI SA LA 3.0 5.0 2.0 SALA 1.0 0.75 GCMS AA TOC 2.0 0.50 2.5 1.0 0.5 1.0 0.25 EDX Particle size analyzer 0.0 0.00 0.0 0.0 0.0 0.0 2.5 Conc. 0.0 2.5 Conc. 6.0 7.0 5.0 5.5 6.0 3.0 4.0 Combine with a Multi Data Registration license to enable LC GC LC GC integrated management of these instruments’ data. Source: Shimadzu Application News No. AD-0153

In summary, the established MRM-based method on a LC-MS/MS platform offers a highly sensitive and selective approach to detect and monitor the presence of porcine-specific markers for semi-quantitation of the amount of pork in processed foods. The established method from sample preparation to LC-MS/MS analysis exhibits excellent reliable sensitivity, and is potentially able to support halal testing in processed food products. Analysis of various food matrices will be performed in the future.

Shimadzu’s Total Support for Food Safety 18 Analytical and Testing Instruments for Food Safety 19 C10G-E020C Analytical and Testing Instruments for Food Safety Analytical and Testing Support Total Shimadzu’s Safety for Food

Shimadzu’s Total Support for Food Safety Type Region and Identification © Shimadzu Corporation, 2018 Defects in Packaging Prevention of Printed in Japan 3655-02814-10AIT Allergens Off-Flavor Analysis of Inspection and Foreign Matter Toxic Metals Food Additives Bacteria Mycotoxins For Research Use Only. Not for use in diagnostic procedures. Not for use in diagnostic procedures. Use Only. For Research Please contact us to check the availability of these not available in your country. that are to products This publication may contain references in your country. products trademarks and trade names of Shimadzu Corporation, its names and logos used in this publication are Company names, products/service used with trademark symbol “TM” or “®”. whether or not they are subsidiaries or its affiliates, whether or not to either the entities or their products/services, trademarks and trade names may be used in this publication to refer Third-party used with trademark symbol “TM” or “®”. they are in trademarks and trade names other than its own. interest Shimadzu disclaims any proprietary change without notice. Shimadzu subject to to you “as is” without warranty of any kind, and are provided The contents of this publication are to the use of this publication. relating or indirect, or liability for any damage, whether direct does not assume any responsibility Thanks to the built-in UniBloc AP integrated aluminum mass sensor and an Thanks to the built-in UniBloc AP integrated a optimized control system, this balance achieves high-speed measurements with and response as quick as 2 seconds. It has been designed for excellent operability, The AP-W series is equipped with a features an easy-to-read organic EL display. function to automatically calculate the weight values required for sample concentration preparation, which supports routine weighing operations. Veterinary Pharmaceuticals Residual Pesticides : Applicable Universal Testing Machine Universal Testing (AG, EZ Test) FTIR Spectrophotometer (FTIR) Atomic Absorption Spectrophotometer (AA) ICP Emission / Spectrometer ICP-MS) (ICP, X-Ray Fluorescence EDX) Spectrometer (XRF, Liquid Chromatograph Mass spectrometer (LC-MS) MALDI-TOF Mass Spectrometer (MALDI-TOF-MS) Spectrophotometer UV-VIS (UV) Gas Chromatograph (GC) Gas Chromatograph Gas Chromatograph Mass spectrometer (GC-MS) Liquid Chromatograph (LC) Shimadzu Balances List of Analytical and Testing Instruments for Food Safety for Food Instruments and Testing List of Analytical 〇 www.shimadzu.com/an/