Recombinant Human Eotaxin (CCL11) Recombinant Human Protein Expressed in E

Catalog # Aliquot Size

E852-40N-20 20 µg E852-40N-1000 1 mg

Recombinant Human Eotaxin (CCL11) Recombinant human protein expressed in E. coli cells

Catalog # E852-40N Lot # G3211-27

Product Description Specific Activity

Recombinant Human Eotaxin (CCL11) was expressed in E. coli cells. The protein accession number is P51671

Gene Aliases

SCYA11, C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11

Endotoxin Level

<1.0 EU/µg of recombinant protein as determined by the LAL method

Formulation Activity was determined by the ability to induce chemotaxis of purified eosinophils at concentrations ranging between 50-100 Lyophilized from as 0.2 µm filtered solution in PBS (pH 7.5) ng/mL.

Reconstitution Protocol Purity

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then Recombinant Human Eotaxin be diluted into other buffers. (CCL11) resolved on a 17% SDS- PAGE gel under reducing Storage and Stability conditions and stained with Coomassie Brilliant Blue G-250

Upon reconstitution, this cytokine can be stored in working Approx. MW 8.5 kDa aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Scientific Background Recombinant Human Eotaxin

Eotaxin belongs to the platelet factor-4 family of chemokines. The (CCL11) gene symbol is SCYA11. The factor is being referred to also as Recombinant human protein expressed in E. coli cells

Eotaxin-1 and has been renamed CCL11. Eotaxin induces Catalog Number substantial accumulation of eosinophils in the skin without E852-40N significantly affecting the accumulation of neutrophils. Eotaxin is Specific Lot Number G3211-27 a potent stimulator of both guinea pig and human eosinophils in Purity 95% vitro. The Eotaxin receptor is a G-protein-coupled receptor Stability 6 mos. at –20oC from date of shipment selectively expressed in human eosinophils. Storage & Shipping Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.