Genomics Data 7 (2016) 187–188

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Genomics Data

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Profiling of microbial community of hot spring based on metagenomic sequencing

Archana Singh, Enketeswara Subudhi ⁎

Centre of Biotechnology, Siksha ‘O’ Anushandhan University, , Odisha, India article info abstract

Article history: hot spring has diverse temperature and pH range varying from 43 °C to 65 °C and 7.83 to 8.10 respec- Received 28 December 2015 tively. Dense foliage around Deulajhari hot spring contributes to the high total organic carbon content (TOC). In Received in revised form 2 January 2016 our experiment we took sediment samples from the two Deulajhari hot springs (S1 and S2) out of the cluster Accepted 4 January 2016 having temperature of 43 °C and 55 °C and pH of 7.83 and 7.14 respectively. Sediment samples were analysed Available online 7 January 2016 using 16S rRNA of V3‐V4 region by amplicon metagenome sequencing. Over 34 phyla were detected in cluster Keywords: S1 and 32 phyla in cluster S2 at the existing physiochemical parameters temperature 43 °C, pH 7.83, −1 Deulajhari electroconductivity 0.019 dSm , and total organic carbon (TOC) 3.80% for S1 and temperature 55 °C, pH 7.14, −1 16S rRNA electroconductivity 0.019 dSm , and total organic carbon (TOC) 0.97% for S2. Existence of a vast number of un- Metagenome resolved sequences 179 out of 292 in S1 and 186 out of 314 in S2 at the genus level emphasizes the significance of TOC our study. Metagenome sequence information for the both clusters S1 and S2 of Deulajhari is available at NCBI, Illumina platform SRA database with accession number SRX1459732 and SRX1459733 respectively. Direct link to the deposited data: www.ncbi.nlm.nih.gov/sra/SRX1459732 www.ncbi.nlm.nih.gov/sra/SRX1459733 © 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

1. Experimental design, material and methods QIIME pipeline. Krona tool (Figs. 1 and 2) [3] was used for plotting krona graph for each cluster. Odisha being bestowed with a range of hot springs located at differ- 333,524 and 611,391 high quality reads were obtained from the ent geographical locations, is a hub for diverse microbial abundance. cluster S1 and S2 respectively and cluster S1 showed the abundance of Eight hot springs present in different districts of Odisha are Chloroflexi (27.65%), Proteobacteria (27.60%), Chlorobi (8.32%), in , Deulajhari and Magarmuhan in Angul, and Acidobacteria (6.49%) and Spirochaetes (5.94%) at the phylum level Badaberena in Khurda, in Ganjam, Boden in Nuapada, and and cluster S2 showed the abundance of Chloroflexi (39.39%), BankholinCuttack[1]. Our study is the first attempt to explore the mi- Proteobacteria (19.53%), Chlorobi (10.83%), OP1(6.16%) and Nitrospirae crobial diversity of Deulajhari hotspring cluster S1(43 °C) and S2(55 °C) (4.96%) at the phylum level. Total 292 genera were reported in cluster using the Illumina sequencing platform. S1 out of which 113 genera were identified while 179 genera still re- Metagenomic DNA isolation was done from the sediment sample of main unidentified and in cluster S2 total 314 genera were reported two clusters S1 and S2 collected from the Deulajhari hot spring (Lati- out of which 128 were identified and 186 genera remain unidentified. tude 20.74199 N, Longitude 84.49206°E) as per Kumar and Khanna, Thus through the diverse knowledge of microbiota in the two clus- 2014 [2].Primers341F,5′CCTACGGGAGGCAGCAG-3′ and 518R, 5′- ters S1and S2 we can further suggest the application of thermophilic ATTACCGCGGCTGCTGG-3′ were used for the amplification of V3–V4 re- bacteria in the diverse field of research. gion of 16S rRNA using 50 ng of metagenomic DNA from each cluster S1 and S2. Minelute column (QIAGE, India) was used for the purification of Acknowledgement amplified PCR product and Illumina GAIIX sequencer (Genotypic Tech- nology, Pvt. Ltd. Bangalore, India) was used for 150 nucleotide paired We are thankful to the Siksha O Anushandhan University for provid- and multiplex sequencing. Bioinformatic analysis was done through ing the infrastructure for carrying out the research work and to the Ge- notypic Technology Pvt. Ltd. (Bangalore, India) for providing the ⁎ Corresponding author at: Centre of Biotechnology, Siksha ‘O’ Anusandhan University, sequencing platform. And we are also thankful to our colleagues for pro- Khandagiri, Bhubaneshwar 751003, Odisha, India. E-mail addresses: [email protected] (A. Singh), viding the needful support during sampling and other technical [email protected], [email protected] (E. Subudhi). assistance.

http://dx.doi.org/10.1016/j.gdata.2016.01.004 2213-5960/© 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). 188 A. Singh, E. Subudhi / Genomics Data 7 (2016) 187–188

Fig. 1. Abundance of bacterial community in S1 spring of Deulajhari metagenome.

Fig. 2. Abundance of bacterial community in S2 spring of Deulajhari metagenome.

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