New Or Noteworthy Plant Collections from Myanmar (3) Caldesia Parnassifolia, Nechamandra Alternifolia, Potamogeton Maackianus and P

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New Or Noteworthy Plant Collections from Myanmar (3) Caldesia Parnassifolia, Nechamandra Alternifolia, Potamogeton Maackianus and P J. Jpn. Bot. 84: 321–329 (2009) New or Noteworthy Plant Collections from Myanmar (3) Caldesia parnassifolia, Nechamandra alternifolia, Potamogeton maackianus and P. octandrus a a b a Yu Ito , Tetsuo Ohi-Toma , Nobuyuki Tanaka and Jin Murata aBotanical Gardens, Graduate School of Sciences, the University of Tokyo, 3-7-1, Hakusan, Bunkyo-ku, Tokyo, 112-0001 JAPAN; E-mail: [email protected] bKochi Prefectural Makino Botanical Garden, 4200-6, Godaisan, Kochi, 781-8125 JAPAN (Received on June 25, 2009) In the course of floristic research of Myanmar, some noteworthy aquatic monocots; Caldesia parnassifolia (Bassi ex L.) Parl. (Alismataceae), Nechamandra alternifolia (Roxb. ex Wight) Thwaites (Hydrocharitaceae), Potamogeton maackianus A. Benn. and P. octandrus Poir. (Potamogetonaceae) were collected. Of these, C. parnassifolia, P. maackianus, and P. octandrus are new to the flora of Myanmar and N. alternifolia has been re-collected after a long period. To compare them with other regional materials, chromosome count for N. alternifolia and chloroplast DNA sequencing for all but P. octandrus were conducted. Key words: Caldesia, Flora, Myanmar, Nechamandra, Potamogeton. With its wealth of plant diversity, Myanmar Materials and Methods (Burma) constitutes a significant component of Materials of Caldesia parnassifolia the Indo-Myanmar hotspot in terms of both land (Bassi ex L.) Parl. (Alismataceae), area and biodiversity (Dijk et al. 2004). Although Nechamandra alternifolia (Roxb. ex Wight) ca. 11,800 species of spermatophyte have been Thwaites (Hydrocharitaceae), Potamogeton reported from Myanmar (Kress et al. 2003), maackianus A. Benn. and P. octandrus Poir. many more are likely to exist (Tanaka 2005). (Potamogetonaceae) were collected on Recently, several new species, new records and expeditions in 2005 and 2008. The first set noteworthy plant collections have been reported of the voucher specimens was retained in (Tanaka et al. 2006a, 2006b, 2007, 2009, Tanaka Tanaing Office, Forest Department, Ministry and Nagamasu 2006). In the course of ongoing of Forestry, Union of Myanmar. The duplicates inventory work in Myanmar we recognized four are deposited in the herbaria of Makino new or noteworthy species of aquatic monocots Botanical Garden (MBK) and the University based on morphological characters, chromosome of Tokyo (TI). Identifications were based on numbers and chloroplast DNA sequences. The their morphological characters and, if available, importance and characteristics of these four chromosome numbers and chloroplast DNA aquatic species are noted below. sequences. —321— 322 植物研究雑誌 第 84 巻 第 6 号 2009 年 12 月 Table 1. Nucleotide sequence variation (substitutions) in the matK region of chloroplast DNA in Caldesia matK (730 bp) * Taxon Accession number Locality 420 728 C. parnassifolia AB506767* Myanmar A A C. parnassifolia EF088140 Austria A — C. oligococca AY952427 China? G G * Sequence obtained in this study. Chromosome observation of Nechamandra Results and Discussion alternifolia was performed using the procedure of Ito et al. (submitted). ALISMATACEAE The chloroplast DNA sequences of matK Caldesia parnassifolia (Bassi ex L.) Parl. from C. parnassifolia, rbcL from N. alternifolia, in Fl. Ital. 3: 599 (1860). rbcL, trnT-trnL, trnL intron, and trnL-trnF Voucher specimen: MYANMAR. Shan from P. maackianus were obtained using the State: Inya Lake, north of Pyndaya, 20˚59´57˝N, procedure of Ito et al. (submitted). Each target 96˚39´59˝E, in deep lake, 1 Dec. 2008, N. region was amplified by polymerase chain Tanaka & al. 080624 (MBK, TI). reaction (PCR) using the following primer pairs: Distribution: Russia, Japan, China, Taiwan, rbcL Z1 (Wolf et al. 1994) and rbcL 1379R Indonesia, East Timor, Vietnam, Thailand, (Little and Barrington 2003) for rbcL of C. Myanmar, Bangladesh, India, Nepal; Europe parnassifolia and P. maackianus; rbcL Z1 and and Africa. rbcL 636R (P. G. Wolf, http://bioweb.usu.edu/ The genus Caldesia is reported from wolf/rbcL%20primer%20map.htm) for rbcL Myanmar for the first time. In Caldesia, four intron of N. alternifolia; RM_749F (Ito et al. species are recognized in the world (Cook submitted) and 8R (Ooi et al. 1995) for matK of 1996). Although the plants collected in this C. parnassifolia; a and b (Taberlet et al. 1991) study lack inflorescences, they are identified for trnT-trnL of P. maackianus; c and d (Taberlet as C. parnassifolia (Bassi ex L.) Parl. based on et al. 1991) for trnL intron of P. maackianus; their leaf characters: ovate leaf blade, deeply e and f (Taberlet et al. 1991) for trnL-trnF of cordate leaf base, and nerves ascending ±60 P. maackianus. Sequences determined in the degree to the veiw. In matK, the sequence of C. present study were registered with the DNA parnassifolia collected in this study is the same Data Bank of Japan (DDBJ), which is linked to as that of C. parnassifolia from Austria although GenBank, and their accession numbers are given there are some missing data for the Austrarian below (Tables 1, 2, 3). specimen (728) and slightly differ from that To understand the geographic range of of C. oligococca (F. Muell.) Buchenau (Table each species, the following literatures was 1). This species is widely distributed in the investigated: Sugawara 1937, Hartog 1957, Hara northern hemisphere and some part of southern and Williams 1978, Cuong and Vidal 1983, hemisphere as well. Leach and Osborne 1985, Chen 1992, Guo and Li 1992, Sun and Wang 1992, Kadono 1994, HYDROCHARITACEAE Rae and Long 1994, Philcox 1995, Cook 1996, Nechamandra alternifolia (Roxb. ex Choi 2000, Kahina 2000, Timokhina 2000, Yang Wight) Thwaites in Enum. Pl. Zeyl.: 332 (1864). 2000a, 2000b, Guseev 2001, Hayens 2001a, [Fig. 1] 2001b, Tzvelev 2001, Matzenko 2002, Wiegleb 2002. Voucher specimens: MYANMAR. Shan State: Near Yae Aye Kan Dam, Yae Aye Kan, December 2009 Table 2. Nucleotide sequence variation (substitutions) in the rbcL region of chloroplast DNA in Nechamandra and Vallisneria Accession rbcL (606bp) Taxon Locality number 6 24 48 51 69 70 94 96 102 111 189 234 242 252 347 367 432 435 525 534 547 558 561 585 N. alternifolia AB506768* Myanmar A A T G G A G G G G A G T G G C A C T G C G G T N. alternifolia U80706 India A A G G T A A G A G A G T G G C G C T G T G T C V. americana U03726 U.S.A.? A G G T T G G C G A G G C A T C A C C A C A T C V. asiatica AB004898 Japan G A G T T A G C G A G A C A G C A C C A C A T C V. rubra EF143004** Australia — — G T T A G C G A G G C A G C A C C A C A T C V. spiralis EF694963 Africa G A G T T A G C G A G G C A G T A T C A C A T C V. spiralis U80712 India? G A G T T A G C G A G G C A G T A T C A C A T C V. sp. DQ859177 Unknown G A G T T A G C G A G G C A G T A T C A C A T C 84No.6 Vol. Journal ofJapaneseBotany V. sp. AF206832 U.S.A.? A G G T T G G C G A G G C A T C A C C A C A T C * Sequence obtained in this study. ** Deposited as Maidenia rubra. Table 3. Nucleotide sequence variation (substitutions, indels and mononucleotide repeats) in the rbcL and trnT-trnF regions of chloroplast DNA in Potamogeton maackianus trnT-trnL *** trnL intron trnL-trnF *** Accession number Locality (891–898bp) (593bp) (408–409bp) rbcL ** trnT-trnL trnL-intron trnL-trnF 167 281 353 374 431 468–478 541–547 855 860 303 60 261–262 AB506769 * Myanmar AB196944 Japan AB506770* AB506771* AB506772* Myanmar T G A T T T (10) TAGTAGA A A A C T (8) EF471044 EF428377 EF432067 China T G A T T T (10) TAGTAGA G – G C T (9) EF471066 EF428401 EF432091 China C G A C C T (11) — A A A T T (8) AB120550 Japan T G A T T T (10) TAGTAGA A A AB120551 Japan T A G T T T (11) TAGTAGA A A * Sequences obtained in this study. ** No sequence variation in rbcL region. 323 *** Mononucleotide repeats (poly-T) are indicated as combination of nucleotide and its number of repeat. Deletions are indicated as "—". 324 植物研究雑誌 第 84 巻 第 6 号 2009 年 12 月 Fig. 1. Voucher specimen of Nechamandra alternifolia (female). December 2009 Journal of Japanese Botany Vol. 84 No.6 325 Fig. 2. Somatic chromosome of Nechamandra alternifolia. Scale bar = 10 μm. Kalaw Township, 20˚35´37˝N, 96˚31´46˝E, in shallow pond, 0.5 m in depth. 26 Nov. 2008, N. Tanaka & al. 080058 (MBK, TI); Shan State: Fig. 4. Distribution of Potamogeton maackianus. Dotted Inya Lake, north of Pyndaya, 20˚59´57˝N, area and solid circles are based upon several floras mentioned in the text. New locality is indicated with 96˚39´59˝E, in deep lake, 1 Dec. 2008, N. open circle. Tanaka & al. 080635 (MBK, TI). Distribution: China, Vietnam, Myanmar, Bangladesh, Sri Lanka, India, Nepal, Yemen and in major herbaria of the world as far as we Sudan. investigated. Although both female and male Chromosome number: 2n = 16 (Fig. 2; sex individuals coexisted at one of two sites, mature unknown). fruits have not been found. The chromosome The monotypic genus Nechamandra is number of the material from one of two sites in distinguished from the related genus Vallisneria Myanmar was revealed as 2n = 16, which is the by the sessile (or very nearly so) female spathe, same as those of previous studies (Fig.
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