DOCSLIB.ORG

Mouse Mammary Tumor Viruses with Functional Superantigen Genes Are Selected During in Vivo Infection (Viral Recombination) T

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Mouse Mammary Tumor Viruses with Functional Superantigen Genes Are Selected During in Vivo Infection (Viral Recombination) T Proc. Natl. Acad. Sci. USA Vol. 92, pp. 4828-4832, May 1995 Immunology Mouse mammary tumor viruses with functional superantigen genes are selected during in vivo infection (viral recombination) T. V. GOLOVKINA*, J. P. DUDLEYt, A. B. JAFFE*, AND S. R. Ross*t *Department of Microbiology/Cancer Center, University of Pennsylvania, Philadelphia, PA 19104-6142; and tDepartment of Microbiology, University of Texas, Austin, TX 78712 Communicated by Philippa Marrack; Howard Hughes Medical Institute, Denver, CO, February 7, 1995 ABSTRACT Mouse mammary tumor virus (MMTV) en- viral genomic RNA. Surprisingly, offspring that nursed on codes a superantigen that is important for viral infectivity in these transgenic animals showed deletion of their cognate vivo. To determine whether superantigen function was re- Vp14+ T cells, with similar kinetics to that caused by wild-type quired for infection by milk-borne MMTV, we created HYB exogenous MMTV(C3H). Sequence analysis of the LTRs of PRO/Cla transgenic mice. These mice produced a full-length, the newly acquired viruses showed that recombination be- packaged viral RNA with a frameshift mutation that caused tween the transgene and endogenous Mtv-1 proviral RNA premature termination of the superantigen protein. Young caused reconstitution of the sag open reading frame. These HYB PRO/Cla mice showed no deletion of their cognate results indicate that there is selective pressure for viruses that V,14+ T cells, although they shed virus in their milLk The encode functional Sag proteins and suggest that Sag activity is nontransgenic offspring of the HYB PRO/Cla mice were required for virus transmission. infected with this virus, since transgene-specific viral tran- scripts were detected in their mammary glands. Surprisingly, these offspring demonstrated the progressive deletion of MATERIALS AND METHODS V114+ T cells characteristic of exogenous MMTV(C3H) in- Plasmids. The plasmid hybrid MMTV (HYB PRO in this fection. Sequence analysis demonstrated that these newly paper) was a gift from G. M. Shackleford, University of acquired viruses had reconstituted superantigen open reading Southern California (7) (Fig. 1). The HYB PRO/Cla trans- frames resulting from recombination between the HYB gene was constructed by partial Cla I digestion of HYB PRO, PRO/Cla and endogenous Mtv-l proviral RNAs. Thus, there filling in the 5' overhang with Klenow DNA polymerase, and is selection during the infection process for MMTVs with religation. The sequence of the mutation created in the 3' LTR functional superantigen genes. is shown in Fig. 1. Although the Cla I digest and fill-in should have generated a 2-bp insertion, only a 1-bp insertion was Mouse mammary tumor virus (MMTV) is a retrovirus that is created. acquired either through the germline as an endogenous virus Generation of Transgenic Mice. Female and male C3H/ or by milk-borne infection (1). Although the ultimate target for HeN MTV- and C3H/HeN MTV' inbred mice and SW MMTV is the mammary gland, cells of the immune system play outbred mice were purchased from the Frederick Cancer a role in milk-borne virus infection. MMTV encodes a viral Research Facility, Frederick, MD. Transgenic mice bearing protein [superantigen (Sag)] in its 3' long terminal repeat the HYB PRO construct have been described (8). The HYB (LTR) (2, 3) that functions in milk-borne transmission (4, 5). PRO/Cla transgene was introduced into C3H/HeN MTV- The virus-encoded Sag protein is presented by antigen- mice by our standard procedure. Transgenic animals were presenting cells (APCs), such as B cells, to T cells bearing identified by Southern blot analysis (8). specific 3-chain variable (Vp) regions of the T-cell antigen Cells and Flow Cytofluorometry. Peripheral blood lympho- receptor. This presentation of Sag causes the proliferation of cytes were stained with titrated amounts of antibodies [rat specific Vp-bearing T cells when it is recognized as foreign (2) anti-CD4 or anti-CD8 labeled with phycoerythrin (GIBCO/ and deletion of such T cells when it is recognized as self (3). BRL) and fluoresceinated rat anti-Vp14 (9) or rat anti-Vp6 Recent data indicate that the role of Sag is to stimulate division (PharMingen)] and analyzed on an electronically programma- and cytokine production by cognate T cells, thereby creating ble individual cell sorter (Coulter) (4). a reservoir of actively dividing T or bystander B cells suscep- RNase T1 Protection Analysis. Total RNA (40 ,ug) isolated tible to MMTV infection (4-6). These infected cells exist at from virgin and lactating mammary glands and a probe high enough levels for a long enough period of time to allow containing a region of the 3' LTR of exogenous MMTV(C3H) the mammary gland, in which cells begin to divide under the were used for RNase T1 protection analysis (8). hormonal stimulation of puberty (at 3-4 weeks of age), to cDNA Library Construction and Screening. RNA was iso- become infected. lated from the virus fraction of milk (10) and a cDNA library Although the importance of the immune system in the was made with the SuperScript plasmid system for cDNA MMTV life cycle is well known, an absolute requirement for and the sag gene in this cycle has not been established. It is possible synthesis plasmid cloning (GIBCO/BRL). The plasmid that there are alternative pathways of MMTV infection that DNAs ofclones that hybridized with a MMTV LTR probe (11) bypass the need for the Sag protein. To test this, we created were sequenced. HYB PRO/Cla transgenic mice carrying a new MMTV pro- Sequence Analysis. The plasmids were sequenced with a virus with a mutation that caused premature termination ofthe Sequenase kit (United States Biochemical). The primers used MMTV(C3H) Sag. The HYB PRO/Cla transgenic females (arrows in Fig. 1) were no. 1, 5'-AATTCGGAGAACTCGAC- produced milk-borne virus containing the transgene as the Abbreviations: LTR, long terminal repeat; MMTV, mouse mammary tumor virus; RT, reverse transcription. The publication costs of this article were defrayed in part by page charge tTo whom reprint requests should be addressed at: Department of payment. This article must therefore be hereby marked "advertisement" in Microbiology/Cancer Center, University of Pennsylvania, 526 CRB, accordance with 18 U.S.C. §1734 solely to indicate this fact. 415 Curie Boulevard, Philadelphia, PA 19104-6142. 4828 Downloaded by guest on September 26, 2021 Immunology: Golovkina et at Proc. Natl. Acad ScL USA 92 (1995) 4829 Mtv-1 R C3H exo I L HYB PRO gag Pol w~~~~~ - 1 2 3 4 "107 cim TCC TCT TAC M CCO CAT CMmTTOT CCT TCA GMATA AGA... S Y K P H R F C PT E I R CUT WITH Cbl FILL4N FIG. 2. Pedigree of the HYB PRO/Cla transgenic mice. 0, Non- LIGATE transgenic females; o, nontransgenic male; o, transgenic female; o, transgenic male. TCC TCT TAC CCO CAT CeC ATT TTO TCC TTC AGAM T AGA MT MT OC TOA S 8 Y K P H R I V F R N R NKN 8 W was used for RNase T1 protection assays (Fig. 3). Expression FIG. 1. Map of the HYB PRO construct with the three viral of the transgene was detected in the mammary glands of both transcripts. The numbered arrows denote the approximate location of strains of transgenic HYB PRO/Cla females (shown for strain the primers used for sequencing of the recombinant viruses. Also t of 340 nt shown is the Cla I frameshift mutation (underlined) introduced into 28; band in Fig. 3, lane 6). The transgene in both the 3' LTR of the HYB PRO/Cla transgene. The boxed sequence 1 2 3 4 5 6 7 8 9 TAA is the premature termination codon. R, EcoRI; C, Cla I. - 404 CTTCC-3' [nt 268-289 in the MMTV LTR (12)]; no. 2, -327 5'-TTGTAAGAGGAAGTTGGCTG-3' (nt 326-307); no. 3, 5'-CTCCTTGGTATGGAAAATCTTTCC-3' (nt 894-871); - 231 and no. 4, 5'-GGACTGTTGCAAGTTTACTC-3' (nt 1203- 1184). Reverse Transcription (RT)-PCR Analysis. Total RNA (20 jig) isolated from lactating mammary gland was reverse tran- - 141 scribed with SuperScript II reverse transcriptase in the buffer supplied by the manufacture (GIBCO/BRL) and a (dT)15 Mtv-1 < primer (Promega). One-fourth of each cDNA preparation was used for PCR with primers specific for exogenous MMTV(C3H) [no. 1 and no. 5 (5'-TAATGTTCTATTAGTC- CAGCCACTGT-3; nt 923-898)] or endogenous and exoge- nous MMTVs [no. 6 (5'-GAGACGCTCAACCTCAATTGA- 3'; nt 507-527) and no. 4], as described (6). After PCR amplification, 20 ,lI of each reaction mixture was digested with Mun I (GIBCO/BRL). The PCR products were analyzed in 1.8% agarose gels. 118 -15 107 440 -> Probe RESULTS HYB PRO/Cla Transgenic Females Produce Virus. We 340 Full-length protection previously created a strain of mice, HYB PRO, with a genet- ically engineered MMTV provirus as a transgene in the FIG. 3. Mice nursed on HYB PRO/Cla transgenic females get C3H/HeN MMTV- inbred background (8). This transgene infected with MMTV. RNase protection analysis was performed on encodes a Sag protein with the same Vp specificity as exoge- RNA isolated from the mammary glands of various lactating and nous MMTV(C3H) virus (Vp14). C3H/HeN MMTV- mice virgin females (see Fig. 2), using the MMTV(C3H)-specific probe (8, were also used to create transgenic mice carrying the HYB 10). Lane 1, tRNA; lanes 2 and 3, lactating mammary glands of PRO construct with a frameshift mutation at aa 113 that C3H/HeN MMTV+ mice; lane 4, virgin mammary gland from the resulted in premature termination of the Sag protein encoded transgenic offspring of a HYB PRO/Cla 28 female (mouse B in Fig. by the 3' LTR (HYB PRO/Cla) (Fig.
Load more

Recommended publications
  • American Punk: the Relations Between Punk Rock, Hardcore, and American Culture
    American Punk: The Relations between Punk Rock, Hardcore, and American Culture Gerfried Ambrosch ABSTRACT Punk culture has its roots on both sides of the Atlantic. Despite continuous cross-fertiliza- tion, the British and the American punk traditions exhibit distinct features. There are notable aesthetic and lyrical differences, for instance. The causes for these dissimilarities stem from the different cultural, social, and economic preconditions that gave rise to punk in these places in the mid-1970s. In the U. K., punk was mainly a movement of frustrated working-class youths who occupied London’s high-rise blocks and whose families’ livelihoods were threatened by a declin- ing economy and rising unemployment. Conversely, in America, punk emerged as a middle-class phenomenon and a reaction to feelings of social and cultural alienation in the context of suburban life. Even city slickers such as the Ramones, New York’s counterpart to London’s Sex Pistols and the United States’ first ‘official’ well-known punk rock group, made reference to the mythology of suburbia (not just as a place but as a state of mind, and an ideal, as well), advancing a subver- sive critique of American culture as a whole. Engaging critically with mainstream U.S. culture, American punk’s constitutive other, punk developed an alternative sense of Americanness. Since the mid-1970s, punk has produced a plethora of bands and sub-scenes all around the world. This phenomenon began almost simultaneously on both sides of the Atlantic—in London and in New York, to be precise—and has since spread to the most remote corners of the world.
    [Show full text]
  • Applied Eugenics
    CHAPTER III DIFFERENCES AMONG MEN While Mr. Jefferson, when he wrote into the Declaration of Independence his belief in the self-evidence of the truth that all men are created equal, may have been thinking of legal rights merely, he was expressing an opinion common among philosophers of his time. J. J. Rousseau it was who made the idea popular, and it met with widespread acceptance for many years. It is not surprising, therefore, that the phrase has long been a favorite with the demagogue and the utopian. Even now the doctrine is by no means dead. The American educational system is based largely on this dogma, and much of the political system seems to be grounded on it. It can be seen in the tenets of labor unions, in the practice of many philanthropies--traces may be found almost anywhere one turns, in fact. Common enough as applied to mental qualities, the theory of human equality is even more widely held of "moral" qualities. Men are considered to be equally responsible for their conduct, and failure to conform to the accepted code in this respect brings punishment. It is sometimes conceded that men have had differing opportunities to learn the principles of morality; but given equal opportunities, it is almost universally held that failure to follow the principles indicates not inability but unwillingness. In short, public opinion rarely admits that men may differ in their inherent capacity to act morally. In view of its almost universal and unquestioned, although half unconscious, acceptance as part of the structure of society, it becomes of the utmost importance that this doctrine of human equality should be examined by scientific methods.
    [Show full text]
  • Danny Wimmer Presents 2021 Festival Calendar
    Danny Wimmer Presents 2021 Festival Calendar: DWP Partners With Inkcarceration Music & Tattoo Festival For July 23-25 Event In Mansfield, OH Louder Than Life Set For September 23-26 With Newly Added Thursday Night Celebration In Louisville, KY Welcome To Rockville Expands To 4 Days And Shifts From Spring To November 11-14 In Daytona Beach, FL Previously Announced Aftershock Set For October 7-10 In Sacramento, CA, Almost Sold Out As the music industry looks to bring live events back to eager fans in 2021, leading independent live entertainment producer and promoter Danny Wimmer Presents is excited to announce its upcoming festival calendar for the year. The event lineup includes the return of fan favorites Louder Than Life and Welcome To Rockville, alongside the previously announced Aftershock, and new to the DWP family, Inkcarceration Music & Tattoo Festival. Louder Than Life, the largest rock festival in America, will take place September 23-26, 2021 at Highland Festival Grounds at KY Expo Center in Louisville, Kentucky and will be adding a Thursday night celebration event to kick off the weekend. All current pass holders will receive admission to the newly added Thursday night kickoff celebration for free. Welcome To Rockville will return with an expanded 4-day festival weekend that furthers its reputation as Florida’s biggest rock experience, and will move to a new weekend for 2021, November 11-14. Rockville will also have a new home, relocating to the Daytona International Speedway in Daytona Beach, Florida. Due to the festival date change from May to November, current Welcome To Rockville ticketholders will have the opportunity to request a refund or use their passes for the upcoming November festival.
    [Show full text]
  • 3. M Portable Scale of Proportions of the Human Body. by W. F. Stanley
    1206 r e p o r t — 1885. 3. M Portable Scale of Proportions of the Human Body. B y W . F . S tanley, F.G.S., F.BJI.S. This instrument is a small thin scale or rule of ivory, of about three inches in length and three-quarters of an inch in width. It is divided on each edge of the two faces by lines which represent the proportions of the human body ; the male on one side, and the female on the other. The lines are marked with the words, crown, eye, chin, shoulder, teat, navel, hand, &e. The scales of proportions which the lines represent are taken from measurements of the diagrams of the assumed perfect human forms given by John Marshall, F.R.S., F.R.C.S., in his work, ‘ A Rule of Proportion for the Human Figure.’ The opposite edge to that on which the pro­ portions are shown is divided into 100 parts in the same space as the height of the body, the tens being indicated by figures. The object aimed at by the use of this proportional scale is to compare any person, or statue, or photograph, with the model of perfect form given by Marshall, or to divide the parts of the body in proportional decimals of the whole for description. The method of using the scale is to hold it up before the eye, facing the object at such a distance that the subtended angle from the two extreme lines on the scale may coincide with the crown and sole of the human form observed.
    [Show full text]
  • FOR IMMEDIATE RELEASE May 3, 2018 Contact: Rody Lopez
    FOR IMMEDIATE RELEASE May 3, 2018 Contact: Rody Lopez, Communications Manager, [email protected], 213-250-4800 x556 Lydia L. Ramos-Mendoza, [email protected], 562-208-1336 Para Los Niños Announces Bellas Artes Gala Para Los Niños Honoring the World Famous KROQ 106.7FM and the Colburn School Evening hosted by KROQ’s Nicole Alvarez -- Presented by Tina & Rick Caruso -- Los Angeles, May 3, 2018—Para Los Niños (For the Children), Skid Row’s longest standing nonprofit organization serving students and families, announced today its Bellas Artes Gala Para Los Niños slated for Wednesday, May 9, 2018 from 6-9:30 PM. Receiving the evening’s top honors are the World Famous KROQ 106.7FM (Corporate Partner of the Year) and the Colburn School, Los Angeles (Cultural Partner of the Year). KROQ’s Nicole Alvarez is serving as the emcee for the evening. Notables from the corporate, philanthropic, foundation, and entertainment industries will gather to celebrate this year’s honorees and the use of art as a teaching and healing tool. Para Los Niños and its Board of Directors are proud to present this 37th anniversary gala at LA Plaza de Cultura y Artes, a champion in sharing the art of the Latino community. Tina and Rick Caruso once again serve as presenting sponsors of the annual gala. “We are delighted to celebrate the critical mission of Para Los Niños,” said Rick Caruso, business leader, philanthropist, and founder/CEO of Caruso, one of the largest privately held private real estate companies in the United States. “The children of Los Angeles deserve every opportunity to be successful leaders and innovators.
    [Show full text]
  • A Musician's Life: New Models for Success in the Music Industry in the 21St Century Sumi Krishnan a Thesis Submitted to the Fa
    A Musician’s Life: New Models for Success in the Music Industry in the 21st Century Sumi Krishnan A thesis submitted to the faculty of the University of North Carolina at Chapel Hill in partial fulfillment of the degree of Master of Arts in Journalism and Mass Communication in the School of Journalism and Mass Communication. Chapel Hill 2010 Approved by: Debashis Aikat Donald Shaw Andreas Preuss ©2010 Sumi Krishnan ALL RIGHTS RESERVED ii ABSTRACT SUMI KRISHNAN: A Musician’s Life: New Models for Success in the Music Industry in the 21st Century (Under the direction of Debashis Aikat) This thesis explores how technology advancements have driven a power shift in the music industry, birthing new business models that musicians as well as the music industry at-large can adopt. It also provides a real-life story of one band’s struggles in the industry today. This thesis project consists of three articles. The first article, “So, You Want to Be a Rock Star?” outlines new models for both musicians and businesses to adopt in order to succeed. The second article, “The Prize at the End: One Band’s Struggle for Fame,” tells the story of a local band, SWASO, struggling to succeed and trying to learn from past mistakes. The final article, “Three Cities to a New Day: Chronicling a Social Media Experiment” picks up with SWASO a few months later and follows the band on a three-city tour as they implement new and innovative social media strategies. iii ACKNOWLEDGEMENTS I am grateful to my thesis chair, Deb Aikat, for his consistent encouragement and support throughout this project.
    [Show full text]
  • The Offspring Spare Me the Details Free Mp3
    The offspring spare me the details free mp3 Spare Me The Details. Artist: Offspring. MB · Spare Me The Details. Artist: Offspring. MB · Spare Me The Details. Buy Original Song The Offspring - Spare Me the Details in Itunes and Amazon For Supporting Artist. Related Song. No related song! Offspring - Spare Me The Details, download free MP3! - download free mp3 music, free video shows, free pictures and photos, mp3 download, free mp3. 10 The Offspring - Spare Me The Details. The Offspring - Spare Me The Details- Live. Live Spare Me. Listen and download free mp3 songs like The Offspring. Buy Spare Me The Details [Explicit]: Read Digital Music Reviews - Start your day free trial of Unlimited to listen to this song plus tens of. Start your day free trial of Unlimited to listen to this album plus tens of millions more . Spare Me The Details [Explicit] The Offspring Stream or buy for $ My girlfriend, my dumb donut Went out to a party just the other night But three hours later and seven shots of. Offspring - mp3 free download, ixnay on the hombre, music stores online Offspring feelings spare me the details why don t you get a job? self esteem. Offspring special delivery, the sound of music mp3 online music store vultures. Offspring free sheet music downloads spare me the details, free downloads of. Direct The Offspring download. Here you could find all mp3s from The Offspring Spare Me The Details. Site offer thousands of mp3 files, fast connection. Offspring come out swinging, music downloads free kill the president, spare me the details.
    [Show full text]
  • SURFING for PUNKS: the INTERNET and the PUNK SUBCULTURE in NEW JERSEY by AARON ROBERT FURGASON a Dissertation Submitted To
    SURFING FOR PUNKS: THE INTERNET AND THE PUNK SUBCULTURE IN NEW JERSEY by AARON ROBERT FURGASON A Dissertation submitted to the Graduate School-New Brunswick Rutgers, The State University of New Jersey in partial fulfillment of the requirements for the degree of Doctor of Philosophy Graduate Program in Communication, Information and Library Studies written under the direction of Professor Linda Steiner And approved by ________________________________ ________________________________ ________________________________ ________________________________ New Brunswick, New Jersey October, 2008 ABSTRACT OF THE DISSERTATION SURFING FOR PUNKS: THE INTERNET AND THE PUNK SUBCULTURE IN NEW JERSEY BY AARON ROBERT FURGASON Dissertation Director Professor Linda Steiner This study examines the influence of the Internet on the punk subculture in New Jersey. Previous research by academics on the effect of the Internet on popular music has centered on the impact of Napster and peer-to-peer networks on the distribution of recordings (Alexander, 2002; Ayres & Williams, 2004; Bishop, 2004; Daniel & Klimis, 1999; Fox, 2004; Mardesich, 1999; & Oberholzer & Strumpf, 2004). However, unexamined is the influence of the Internet on a specific music subculture, punk. This is important because the subculture is occupied mainly by youth engaged with both the Internet and punk music. The Internet's influence on the punk subculture is a particularly important area of study because the Internet alters how participants in the subculture (punk fans, bands and independent labels) communicate with one another, as well as changes the distribution of punk artifacts and information. ii Central to this study is the history of the major labels' dominance over the distribution and promotion of punk music, a domination examined via critical political economy theory.
    [Show full text]
  • Drumsticks • Brushes • Mallets
    2018 DRUMSTICKS • BRUSHES • MALLETS • SPECIALTY STICKS • MARCHING • ENSEMBLE • PRACTICE PADS • ACCESSORIES Vater’s undivided focus is to give you the highest quality drum sticks, specialty sticks and drum MANUFACTURING accessories in the world. MANUFACTURING THE FACTS - Vater has several wood suppliers. Our partners adhere to Vater’s strict specifications and quality terms, providing Vater with the very best Hickory and Maple Nellyris "Didi" Negron for making drumsticks. CIRQUE DU SOLEIL: Peter Wilson AMALUNA - Hickory only grows in the United States and is the best DANIELLE BRADBERY KEG 5A wood in the world to produce striking tools. (Hammers, 5B Ax Handles, and yes... drumsticks). Hickory absorbs a great amount of shock. We all know the saying: - The best process for producing very white Hickory is to use low temperatures when Kiln drying so not to "pink" the wood. The Kilns start drying at 85 degrees and are slowly increased but never exceed 105 degrees. "They don't make them like they used to." - The Kiln drying cycle time that produces the best results is 2 1/2 weeks. - Vater's Hickory is stress released which is done by Scott Pellegrom slowly raising the temperature at the end of the drying Well... We still do! Joe Travers INDEPENDENT cycle for 8 hours. This relieves the stress in the wood JOE SATRIANI JAZZ RIDE and produces straight sticks that stay straight. JOSH FREESE'S H-220 - Vater uses the 2 best Hickory species for it's drumsticks which are Shell Bark and Shag Bark Check out our factory tour video on YouTube! Vater Drumsticks are still made the same as they've Hickory YouTube.com/VaterPercussionUSA always been, resulting in a consistent and durable - Vater uses a dowel moisture content specific to "striking" tools (10%-12%).
    [Show full text]
  • In Tune Dinosaurs Still Rockirv Billboards Associated Press by Daniel M
    Fri., Dec. 18, 1998 The Gazette, Cedar Rapids, Iowa 3W In tune Dinosaurs still rockirV Billboards Associated Press By Daniel M. Shapiro Following are weekly charts for the Gazette staff writer FAST TAKE nation's best-selling recorded music t takes a special kind of from next week's issue of Billboard person to don cat makeup, magazine. Reprinted with permission. wear a Gothic S&M outfit WHO: Kiss, with Econoline I Crush and soar on a 3-ton contrap­ TOP SINGLES tion above adoring breast- WHEN: 8 p.m. Saturday baring women. Naturally, WHERE: Five Seasons Center | "I'm Your Angel," Kelly & Dion "Nobody's Supposed to be Here," Deborah Cox Peter Criss wouldn't trade ADMISSION: $39.50, through Ticketmaster, 363-1888, "Lately," Divine his job for anything. | "From This Moment On," Shania Twain and the Five Seasons A few years ago, though, . Baby One More Time," Britney Spears Criss wasn't playing drums Center box office, 362- "Because of You," 98 Degrees with Kiss. He was on board 1729 I "Doo Wop," Lauryn Hill when the band formed in "Love Uke This," Faith Evans "Have You Ever?," Brandy 1972, but left in 1980. Long­ zoom at them. "Lullaby," Shawn Mullins standing differences among Such an odd comic-book I members had prevented the world might seem inappro­ TOP ALBUMS original group from getting priate for a 51-year-old dad, - back together. When a 1996- but Criss' daughter never "Double Uve," Garth Brooks 97 reunion tour came about, had a problem with it. I "These are Special Times," Celine Dion a lot of people were sur­ 'N Sync," 'N Sync "She did a report in "Spirit," Jewel prised, including Criss.
    [Show full text]
  • TDJ Issue Thirteen.Pdf
    A bit like a trail of breadcrumbs, we’ve dispersed the TDJ logo throughout the magazine. If it all gets a bit much, you can click it at any point to return back to the contents page. You can also click the title of any article on the contents page to skip straight to it. Ads are interactive too, so if you see something you like, you can click it for more information. Contents Masthead 10 In Step BYOS’ Ralph Nader 19 Josh Freese Insert Coin 29 With Modulation Tortoise’s Dan Bitney, John McEntire & John Herndon Subscribe 39 Barn Find A Conversation with Benny Greb Win! 49 Dennis Davis CONTENTS Bowie, Benson and Beyond Issue Thirteen, Summer 2016 55 Process Architecture Working in a Drum Workshop 68 Working Title Bloc Party’s Louise Bartle Editor: Tom Hoare [email protected] Design: Luke Douglas [email protected] Social: Vicky Batrak Proof Reader: David Smith Contributors: Joe Wong, Julia Kaye Photographers: Carlos Goethe, Michael Baumann Max Respect: Marion Rebmann, Richard Vos, Tyler Cannon, Dan Wiseman, Matt Budd, Luis Cardoso, Ellie Shapiro, Rob Grad, Katie Bursey, Kate Darracott, Elspeth Leadbetter, Beto Benitez, Flora Hodson, Elaine Smith, Julia, Greg, Ben Smith, Thomas Barth, Ryan Swift, Colin Roberts, Norbert Saemann, Jessica Linker, Michele Melrose, Tracy Hoeft, DW Drums. Cover Illustration: Christopher Neal MASTHEAD Issue Thirteen, Summer 2016 Copyright © The Drummer’s Journal 2016 The proprietors and contributors to The Drummer’s Journal have asserted their right under the Copyright Designs and Patents Act 1988 to be identified as the owners and authors of this work.
    [Show full text]
  • "Rock Against Reagan": the Punk Movement, Cultural Hegemony, and Reaganism in the Eighties
    University of Northern Iowa UNI ScholarWorks Dissertations and Theses @ UNI Student Work 2016 "Rock against Reagan": The punk movement, cultural hegemony, and Reaganism in the eighties Johnathan Kyle Williams University of Northern Iowa Let us know how access to this document benefits ouy Copyright ©2016 Johnathan Kyle Williams Follow this and additional works at: https://scholarworks.uni.edu/etd Part of the Cultural History Commons Recommended Citation Williams, Johnathan Kyle, ""Rock against Reagan": The punk movement, cultural hegemony, and Reaganism in the eighties" (2016). Dissertations and Theses @ UNI. 239. https://scholarworks.uni.edu/etd/239 This Open Access Thesis is brought to you for free and open access by the Student Work at UNI ScholarWorks. It has been accepted for inclusion in Dissertations and Theses @ UNI by an authorized administrator of UNI ScholarWorks. For more information, please contact [email protected]. Copyright by JOHNATHAN KYLE WILLIAMS 2015 All Rights Reserved “ROCK AGAINST REAGAN”: THE PUNK MOVEMENT, CULTURAL HEGEMONY, AND REAGANISM IN THE EIGHTIES An Abstract of a Thesis Submitted in Partial Fulfillment of the Requirements for the Degree Master of Arts Johnathan Kyle Williams University of Northern Iowa May, 2016 ABSTRACT Despite scholars’ growing interest in the cultural movement known as punk, there has been a lack of focus on the movement’s relationship to its historical context. Punk meant rebellion, and this research looks at how the rebellion of the American punk movement during the eighties [1978 to 1992], was aimed at the president Ronald Reagan. Their dissent, however, was not only directed towards Reagan, but the culture that he encompassed.
    [Show full text]