ROSLINREPORTER
Issue 29 Summer 2014
Highland Show 2014 What happened to the first half of 2014!? Well, to find out how we’ve been keeping busy at The Roslin Institute, read on…
Secretary of State for Scotland Alistair Carmichael swabs the Institute chicken supervised by l/r Pete Kaiser, David Argyle and Kate Harrison
he Roslin Institute was once again at the of its celebration of the 20 years it has been TRoyal Highland Show this year alongside funding “Excellence in Bioscience” in the UK. Easter Bush colleagues from the Royal (Dick) The exhibit, called “Flu Fighters” showcases School of Veterinary Studies (R(D)SVS). current flu research including GM animals, This year the Institute brought two main novel approaches to vaccines and hands-on exhibits to the Show to illustrate the diagnostic tests and host/virus genetics. It kind of research that is underway at Easter explains scientific terminology e.g. ‘H5N1’, Bush. The first exhibit highlighted the cross- demonstrates how flu transmits from animals campus research underway in the study of to humans and highlights the challenges of dog genetics. We have a number of projects preventing large-scale outbreaks. across campus that study the genetics of hip Featuring an interactive computer game and dysplasia, glaucoma and heart/lung disease a chicken that visitors can swab to test for flu in dogs among other conditions. Along with The Roslin Institute and The Pirbright Institute Inside this issue: information about the research there was will also be running the activity at the Surrey an activity that demonstrated the genetics County Show and The Roslin Institute Open behind coat colour in Labradors. Day, part of Midlothian Science Festival Director’s Message Page 4 The second exhibit focussed on influenza viruses and the science behind the Science News Page 5 varied approaches to combating flu being developed at The Roslin Institute. This Other News Page 14 exhibit is a joint venture with colleagues before the main presentation at the Great from The Pirbright Institute and it is being British Bioscience Festival (http://goo.gl/ Recent Publications Page 31 funded by the Biotechnology and Biological aL47zp) being organised by BBSRC in Sciences Research Council (BBSRC) as part London (November 2014). 2 The Royal Highland Show was as always a huge success and we’d like to thank the organisers, all our volunteers and of- course our many thousands of visitors who we had a great time sharing our science with and hearing what they think about what we do.
Roll on 2015 !
3 Directors message This newsletter is going out following a period of horizon scanning both as part of an internal review process and in the wider development of the future research strategy of The Roslin Institute with our Easter Bush Research Consortium partners at the Royal (Dick) School of Veterinary Studies (R(D) SVS), Scotland’s Rural College (SRUC) and Moredun Research Institute. I mentioned in the previous Roslin Reporter that we have grand plans for the University of Edinburgh’s Easter Bush Campus. In March this year the University and SRUC announced that they are exploring opportunities for closer alignment of the two organisations. The Roslin Institute already has a strong and productive relationship with SRUC and I am delighted that With BBSRC CEO Professor Jackie Hunter we are going to be working with it to expand the opportunities that greater alignment will afford. drive sustainable improvements in animal it was enormously useful and has given us Our campus development continues with health and livestock productivity and I look much to consider ahead of the more formal plans being developed for The Centre forward to working with other industry partners review that will be carried out by the BBSRC for Comparative Pathology (integrating as our research portfolio expands. later this year. diagnostic, training and imaging resources for The Easter Bush Innovation Centre Building We were also delighted to welcome to The human, experimental animals, livestock and will provide further facilities that will be Roslin Institute, in May, Professor Jackie companion animals) and also the Large Animal attractive to our partners, research centres Hunter, the BBSRC chief executive officer. It Research and Imaging Complex. Bringing and commercial tenants as well as providing was Jackie’s first visit to the Institute since she such facilities to the Easter Bush Campus as the hub to the campus. The stage E design took up post eight months ago and we spent part of our wider campus development plans has now been completed and will be going out two days giving her a tour of the campus (more about these at www.easterbush.mvm. for tender for construction very soon. as well as showcasing some of our main ed.ac.uk) enables us to continue to pursue our Creating the right infrastructure for our work research areas. The latter task was entrusted vision for the campus to deliver excellence in is key to providing the best environment in to our students and postdocs who, once again, animal science and food security. which to carry out our research. At the end proved themselves really exceptional young Development of the National Avian Research of March, our Science Advisory Board (SAB) scientists who will certainly be highflying Facility (NARF) at Easter Bush Campus was spent a day at the Institute reviewing our scientists as their careers progress. I’m sure one of the selling points that resulted BBSRC-funded strategic programmes and Along the same train of thought I will finish in a three-year research agreement being providing us with feedback as to how to this message with a sincere congratulations to signed between Cobb-Vantress, Inc., a global refine the research during the second half all the postgraduate students who presented industry leader in poultry genetics, and The of the funding period as well as beginning to their research at the R(D)SVS and Roslin Roslin Institute (more later in this newsletter). identify some of the key strategic areas that Institute Postgraduate Student Day at the end The joint partnership with Cobb is an excellent we should be considering moving forward. I’d of April. We have some seriously talented example of the kind of industrial interactions like to thank all the members of the (SAB) young scientists establishing their skills here that allow The Roslin Institute’s research to for taking the time to undertake that review; and I am, as ever, prodigiously proud of them.
4 Science News
Tackling virtual infectious disease rofessor Tom Freeman together with that it will be possible to eliminate drug and Pcolleagues at The Roslin Institute, vaccine candidates that are less likely to be University of Edinburgh, Italy and Ireland has successful thereby reducing the numbers of won a proof-of-concept award through the animals currently used in such tests. NC3Rs’ CRACK IT Challenges competition. The funding is part of the CRACK IT This funding scheme is aimed at employing Challenges programme, led by the UK’s new ways of working that will eventually National Centre for the Replacement, reduce the number of animals needed for Refinement and Reduction of Animals in research to discover and develop new drugs Research (NC3Rs). The funding programme to fight infectious diseases. is an open innovation platform that was set Professor Freeman’s project aims to create a up in 2011 to solve scientific and business Virtual Infectious Disease Research platform problems with a 3Rs theme. Tom to simulate the course of an infection and Professor Freeman said of the award, “We the body’s immune response. The work will have been developing the tools to do this initially focus on the computer modelling of kind of work at The Roslin Institute for a complex interactions between an immune cell number of years, such as the cell network called a macrophage and its infection by the analysis tool, Biolayout Express3D funded influenza virus that causes flu. by the Biotechnology and Biological Sciences Control of infectious diseases is a key priority Research Council. in human and veterinary medicine. A study to The new NC3Rs funding provides us with an test the efficacy of new antibiotics or vaccines excellent opportunity to really establish how can typically require around 100 animals for these approaches can be used in an industrial each drug candidate that is tested. context and in a directly beneficial way.” This project will develop new computing tools Funding for 2014 CRACK IT Challenges and ways of working with the aim of predicting come from the Technology Strategy Board’s the efficacy of drugs. In this way the hope is Small Business Research Initiative.
by New recruits to the BRF Dave Davies & Clare Pridans ou can see all the lambs as you drive to from a third clone expected to deliver at the YEaster Bush and at The Roslin Institute we beginning of June. have had the pitter-patter of even tinier feet! Rat The generation of the genetically modified transgenesis is a new project for us and over ESC (Csf1r-/-) was performed by Linda the last six months we have gained invaluable Sutherland in Tom Burdon’s lab and forms experience and are now reaping the benefits. part of Clare Pridans’ research (David Hume Our first three chimeras were created through lab) to investigate the role of Csf1 in postnatal embryonic stem cell (ESC) technology. growth and development. Genetically modified ESCs derived from brown rats (Dark Agouti) were injected into developing embryos from albino donor (Sprague Dawley) rats. The injected embryos were then surgically implanted into a pseudo pregnant recipient rat. If you look closely, Ziggy has 1 red eye and 1 dark eye. As we type this report we have had another litter containing chimeras from a second ESC clone and a further two pregnant recipients (l/r) Stardust, Braveheart and Ziggy
5 Roslin Institute researchers £179,000 win arthritis research UK funding
Katherine Staines the Post Doc Researcher working on Osteoarthritis at The Roslin Institute
edical research charity Arthritis Research been explored. The team will examine first the MUK is funding new a study at The Roslin role of this protein in the disease and then look Institute that offers new hope for people at whether progressive bone thickening can with osteoarthritis by preventing the painful be limited by increasing levels of E11.” excessive bone thickening often seen in the Osteoarthritis is the most common form of joint joints of people with the condition. disease worldwide. In the UK, around a third Professor Colin Farquharson’s research team of people aged 45 years and over, totalling has been awarded £179,000 from the charity 8.75 million people, have sought treatment to carry out a three-year study investigating from their GP for the condition. The condition bone changes around joints that take place in causes pain and stiffness in the joints due to osteoarthritis. Researchers hope the findings cartilage at the ends of bones wearing away. will provide clues for how to stop disease Professor Alan Silman, medical director progression and even provide a new treatment. at charity Arthritis Research UK, said: Progressive thickening of the bone underneath “Osteoarthritis has a significant personal X-Ray of the excessive bone the joint cartilage is a hallmark of osteoarthritis impact on people’s lives as it causes chronic thickening (sclerosis) in an and is thought to trigger cartilage destruction, pain and joint dysfunction. osteoarthritic knee joint leading to further progression of the disease. “This study offers hope of an improved The team will look at whether a protein, called understanding of the bone changes in E11, is responsible for making bone cells that osteoarthritis, which has the potential to lead are capable of producing a protective protein, to the development of new, more effective called sclerostin, which acts as a natural shield treatments to combat and prevent this disease.” against bone thickening in osteoarthritis. If so, Arthritis Research UK is the leading authority they hope to harness its action for a potential on arthritis in the UK, conducting scientific and new drug therapy. medical research into all types of arthritis and Professor Farquharson said, “Existing related musculoskeletal conditions. Receiving treatments for people with osteoarthritis no government funding, it is the UK’s fourth are extremely limited, partly due to a lack largest medical research charity and the of understanding of the disease. New and only charity solely committed to funding high effective therapies for this condition, which is quality research into the cause, treatment and often very disabling for people, are therefore cure of arthritis. very much needed. However to achieve this, it is necessary to first identify the key factors involved in disease onset and progression. “The role of E11 in osteoarthritis has not yet
6 ‘protect against any strain of the flu’ oslin Institute scientists have, with “Our research demonstrated that treatment RSt. Andrews University collaborators, with the biologics results in reduced virus developed a novel treatment that could protect replication in a mouse model and protected against any strain of the flu. against lethal influenza infection. We In an international effort, the scientists also showed that the biologics bind to the involved say that the preventative treatment epithelium of the lungs in infected mice.” could be used as a ‘frontline defence’ before She continued, “This treatment provides an an effective flu vaccine is developed. Leading exciting approach to combat the threat of influenza experts say the new development novel influenza infections.” is ‘very exciting and potentially of great Influenza remains a constant worldwide importance in this era’. health threat with annual epidemics claiming The BBSRC and MRC-funded research up to 500,000 lives each year according to the was led by Professor Garry Taylor and Dr World Health Organisation. The emergence Helen Connaris in the Biomedical Sciences of new strains from birds in recent years has
New treatment could New treatment Research Complex at St Andrews. revealed the remarkable ability of the virus to Professor Taylor said, “We have developed an cross species barriers and to pose pandemic alternative host-targeted approach to prevent health threats. influenza by synthesising novel proteins, or While vaccines are the cornerstone of biologics, that are designed to mask specific prevention, these are not always effective sugar molecules that line the respiratory tract. and take time to develop in quantities needed “The influenza virus, and indeed other to treat whole populations. Antivirals are respiratory pathogens, needs to bind to these available, but the very recent Cochran report sugars to gain entry to our cells to start the has highlighted the limited effectiveness of infection process.” Tamiflu despite the UK government having The novel method was tested in mice spent almost £0.5billion on stockpiling the by collaborators Dr Bernadette Dutia and drug. Professor Tony Nash of The Roslin Institute For more see: Prevention of influenza by at the University of Edinburgh and at St Jude targeting host receptors using engineered Children’s Research Hospital in Memphis. proteins. 2014. PNAS: www.pnas.org/cgi/ Dr Dutia said of her involvement in the study, doi/10.1073/pnas.1404205111
Bernadette Tony
7 Breeding cattle that are more resistant to bovine oslin Institute scientists have reported tuberculosis Rthat it should be possible to use genetic markers (or SNPs) to breed cattle that are more resistant to infection with Mycobacterium bovis. In a recent open access paper published in Heredity the Roslin team, with colleagues from the Agri-Food and Biosciences Institute (AFBI) and Queen’s University Belfast, described how they applied SNP chips to interrogate the bovine genome for differences in the genetic ability of cattle to resist the major pathogen, M. bovis. Their research could lead to new ways to by Liz Glass control bovine tuberculosis (bTB) by breeding for enhanced resistance to infection. Despite many decades of costly statutory controls of regular skin test and slaughter policies to try and eradicate bTB in the UK and extremes of phenotypic difference i.e. cases It is anticipated that these could be directly Republic of Ireland, M. bovis continues to have were confirmed as infected with M. bovis and applied by the cattle industry to select cattle a huge impact on the health and welfare of controls were matched animals that reacted for increased bTB resistance. The team also livestock, and farm profitability. The pathogen negatively to repeated skin tests. The cases plans to whole genome sequence high and has a wide host range and poses a risk not only and controls (~ 1500 cattle) were genotyped low risk cattle aiming to improve the accuracy to cattle but other livestock, domestic animals at more than 600,000 markers (SNPs) across of the genomic predictors. These predictors and wildlife, and in the developing world, to their genomes, and by combining all of the would be highly advantageous as the progress humans as well. differences detected, almost 25% of the of improving resistance could be fairly rapidly observed variation in phenotype between the implemented by the dairy industry and farmers two groups was accounted for by their genetic and should also reduce the infection levels at differences. They were also able to tentatively the same time. locate some of the differences to specific loci on The aim is to more precisely identify loci the genome that might begin to explain some of that control resistance to M. bovis infection, the mechanisms of the genetic resistance. information that could be valuable in designing These results suggest that there is a great additional control strategies. potential to apply genetics to improve the ability The initial study was funded by the BBSRC as of cattle to fight off this insidious pathogen. part of its CEDFAS initiative. The new funding However further research is now needed and has come from the BBSRC through the Animal The pathogen very cleverly hides in one of the the team at The Roslin Institute together with Health Research Club and involves the teams principle cells of innate immune defence - the their AFBI colleagues and new partners from at The Roslin Institute, AFBI and SRUC (see: macrophage - where it can manipulate and SRUC have recently obtained further funding to Genomic Selection for Bovine Tuberculosis hide from the host response, causing a chronic apply their findings and develop robust genomic Resistance in Dairy Cows). respiratory disease. There is no licenced predictors. vaccine for bTB. If bTB is not brought under Their plans include genotyping of ~1000 control, it is estimated that it will cost the UK tax- additional bTB-phenotyped cattle samples payer over £1 billion in the next decade, with from their unique “biobank” collected under many 10,000’s of cattle slaughtered, causing the previous grant. These will be combined considerable suffering of animals and farmers. with the data reported in the current paper The genome wide association study involved as well as with SNP chip genotypes linked to the collection of 1000s of samples from bTB phenotypes derived from statutory data in Holstein-Friesian cattle from high incidence the UK and Republic of Ireland. Together the herds in Northern Ireland which ensured that enhanced data set will significantly increase the both cases and controls were equally likely power of their earlier study. The results will be to have been exposed to the pathogen. It is used to calculate genomic estimated breeding A new post-doctoral researcher, Samantha important to emphasise that the cases and values (GEBVs) and lead to the development Wilkinson, has been appointed to take the controls were carefully defined as representing of genomic predictors of bTB infection liability. study forward.
8 Roslin Institute researchers receive Grand Challenges Explorations Grant
Ivan Liam he Roslin Institute at the University of parasites called African trypanosomes, which TEdinburgh announced today that it is a Grand cause life-threatening disease in both animals Challenges Explorations winner, an initiative and humans in sub-Saharan Africa. funded by the Bill & Melinda Gates Foundation. Currently there are very limited prospects for Dr Liam Morrison and Professor Ivan Morrison new drugs or vaccines and new approaches to will pursue an innovative global health and controlling these parasites are required urgently. development research project, titled “Exploiting a This Grand Challenges Explorations project novel bovine antibody subset as a trypanocide”. aims to develop therapies based on a unique Grand Challenges Explorations (GCE) funds type of cow antibody, the shape of which means individuals worldwide to explore ideas that can they can potentially target specific areas of the break the mould in how we solve persistent trypanosome’s surface that are inaccessible to global health and development challenges. The other antibodies, therefore offering the prospect Roslin Institute project is one of over 50 Grand of exploiting these antibodies by vaccination or Challenges Explorations Round 12 grants using them as therapeutic agents. announced today by the Bill & Melinda Gates Grand Challenges Explorations is a US$100 Foundation. million initiative funded by the Bill & Melinda To receive funding, The Roslin Institute team Gates Foundation. Launched in 2008, over and other Grand Challenges Explorations Round 950 people in more than 55 countries have 12 winners demonstrated in a two-page online received Grand Challenges Explorations grants. application a bold idea in one of five critical global The grant program is open to anyone from heath and development topic areas that included any discipline and from any organization. The agriculture development, behaviour change initiative uses an agile, accelerated grant-making and looking into animal and human health. process with short two-page online applications Applications for the next Round will be accepted and no preliminary data required. Initial grants starting September 2014. of US$100,000 are awarded two times a year. Liam Morrison and Ivan Morrison are Successful projects have the opportunity to investigating new prospects for combating receive a follow-on grant of up to US$1 million.
9 New maps for navigating the genome of genes that are required to build muscle and unveiled by scientists bone. Another Roslin Institute study used the atlas to investigate the regulation of genes in cells of the immune system. cientists at The Roslin Institute are part The FANTOM5 project included major Sof an international effort that has built the contributions from The Roslin Institute, which clearest picture yet of how our genetic material is is funded by the Biotechnology and Biological regulated in order to make the human body work. Sciences Research Council, and the Medical They have mapped how a network of switches, Research Council Institute of Genetics and built into our DNA, controls where and when Molecular Medicine at the University of Edinburgh. our genes are turned on and off. Professor David Hume, Director of The The Roslin Institute and other University of Roslin Institute and a lead researcher on Edinburgh scientists played a leading role in the project, said: “The FANTOM5 project the international project – called FANTOM5 – is a tremendous achievement. To use the which has been examining how our genome analogy of an aeroplane, we have made a holds the code for creating the fantastic leap in understanding the function of all of the diversity of cell types that make up a human. parts. And we have gone well beyond that, The three year project, steered by the RIKEN to understanding how they are connected and Center for Life Science Technologies in Japan, control the structures that enable flight. has involved more than 250 scientists in more “The FANTOM5 project has identified than 20 countries and regions. new elements in the genome that are the The study is a step change in our targets of functional genetic variations in understanding of the human genome, which human populations, and also have obvious contains the genetic instructions needed to applications to other species.” build and maintain all the many different cell The researchers are now establishing the new types in the body. All of our cells contain the technology developed in Japan at the University same instructions, but genes are turned on of Edinburgh’s high-throughput genomics and off at different times in different cells. facility, Edinburgh Genomics, to enable rapid This process is controlled by switches – called access for researchers based in the UK. promoters and enhancers – found within the genome. It is the flicking of these switches that makes a muscle cell different to a liver or skin cell. The team studied the largest ever set of cell types and tissues from human and mouse in order to identify the location of these switches within the genome. They also mapped where and when the switches are active in different cell types and how they interact with each other. The consortium published a series of papers describing its findings, including a pair of landmark papers in the journal Nature. In a separate study, researchers at The Roslin Institute used information from the atlas to investigate the regulation of an important set
10 News from NARF! by Alan Hart (NARF Coordinator)
nother 6 months have gone by since The usual teething troubles with building Areporting in this illustrious journal. I management systems are mostly resolved and am happy to report that the National Avian Adrian and his team are settling in to their new Research Facility (NARF) is progressing well. home well. I would like to take this opportunity to thank all involved especially Adrian Greenwood Sherman, Kim Bernard, Rhona Mitchell, The Greenwood Building is now fully Frances Thomson, Margaret Murray, Moira operational. The birds were all moved across Hutchison, Willie Stewart and David Pryde. from the old site the week commencing 13th Their hard work through this transition period January 2014 and, with the usual dedication has provided the birds with the best possible and expertise from all Greenwood staff and care. We plan to bolster our staff numbers in Dryden farm staff, the birds were moved with the Greenwood to provide much needed help as Alan the minimum of stress and are now settled in the new pen system is proving to be much more to their first-class accommodation. time consuming so an extra member of staff is required.
Flock management Other Greenwood news A new flock management database is being Chicken semen cryopreservation is also developed by Trevor Paterson and Andy Law. progressing with our first success using the This should help us keep track of all birds, glycerol method. We managed to achieve provide ease of collating information for Home fertilisation on our first attempt! Much work is Office returns and also keep track of financial still to be done to perfect the method as n=1 is reporting. We are still in the testing phase at not sufficient to claim a robust technique. the moment, but once fully operational users Many thanks to Rhona Mitchell for her expert will be able to view their projects online. help and to all in the Greenwood for putting 66 hrs Incubation up with my daft questions regarding chickens, semen collection and artificial insemination. Bumstead
11 For those that wander by the west end of the progress and build quality. A prototype items entering the facility are either fumigated the campus you will have noticed that the pen and a mock up room were on view on the or in the case of humans thoroughly cleaned Bumstead Building is moving on a pace! day and all were approved. A new autoclave by showering before entry. Kim and her staff With all internal walls erected the rooms are is being constructed by BMM Weston and an will be the cleanest on campus come the New all taking shape and Miller construction are off-the-shelf VHP machine has been ordered Year! turning their attention to the finishes. from Tecniplast. These two new items are vital Exciting times are ahead for both the Greenwood The Home Office inspectors were on site at to maintain a clean environment inside the and Bumstead as we develop NARF to become the end of April and they were impressed with positive pressure building by ensuring that all the premier facility in the world.
40 years of excellent service! right eyed and bushy tailed Margaret Margaret should be praised for her long BMurray started her career as an animal service and for providing her skills to The technician joining The Roslin Institute in one Roslin Institute over the years allowing of its previous guises on Monday 17th June students, post-docs and senior scientists alike 1974. After her first week her mother enquired to develop their programmes of research. how it was all going and she said ‘I will stick at Many congratulations Margaret and lang may it for a year and then see!’ yer lum reek!* 40 years later Margaret is still going strong. During that time she has provided first class *Lang may yer lum reek is Scottish slang and care to the animals in her charge, be that translates to Long may your chimney smoke. chicken, quail, turkey, rabbit or mouse. Mid- It’s an old tradition in Scotland to take a piece career she thought of leaving but a chat with of coal with you when visiting friends and the then Director, Grahame Bulfield, provided family on New Year’s Eve. The coal is placed the opportunity for her to stay and do what she on the fire to keep it burning and to wish the does best, looking after the animals in her care. hosts long life and prosperity.
12 Cobb-Vantress and The Roslin Institute partnership three-year research agreement between disease tolerance and disease resistance. ACobb-Vantress, Inc., a global industry Applications for the first tranche of funding leader in poultry genetics, and The Roslin have been submitted and will be awards will Institute will facilitate collaboration on avian be made in due course. disease resistance, genome analysis and genome preservation using the state-of-the- art facilities provided through the National Avian Research Facility (NARF). Cobb is putting almost $1 million into avian research programs at The Roslin Institute to identify innovative ways to improve avian health as well as developing unique $1Mtechnologies to understand and preserve the current and heritage poultry genomes. The collaboration will support research by graduate students and is for an initial three years, with potential for renewal on projects such as investigating DNA markers in the genome, targeting some of the more difficult to select for traits such as avian immunity,
Pete Kaiser presents 2014 Gordon Memorial Lecture genetics of disease resistance. His studies over more than two decades have led to a greater understanding of the avian immune system and how it differs from mammals, of host-pathogen interactions and of genes involved in disease resistance pathways in birds. Pete’s seminar was entitled ‘Understanding host-pathogen interactions in the chicken: prospects for disease control in the post- genome age’ and highlighted the major findings from his work but also that of other Roslin scientists, particularly in genetics, genomics and transgenic technologies. He also took the opportunity to describe what we are trying to build with the National Avian Research Facility here at The Roslin Institute. Pete said ‘It was a great honour to have one’s work recognised by one’s peers and to receive this award, particularly when I look back at the n honour of Dr Robert Fraser Gordon, an distinguished list of previous award winners. To Ieminent poultry veterinarian and scientist who be able to acknowledge the huge contribution set up the Houghton Poultry Research Station, of my excellent team of students and scientists, the Gordon Memorial Lecture is given each year past and present, was very satisfying. I am at the Annual Spring Meeting of the UK Branch fortunate to work in an area of science that of the World’s Poultry Science Association. is of both intellectual interest and practical NARF’s Professor Pete Kaiser was invited to relevance, with friends and colleagues who present the 2014 Gordon Memorial Lecture. Like both support and challenge me daily, and at an Bob Gordon, Pete has dedicated his research Institute that is the best place in the world to career to understand all things avian, with deliver that science. And remember, chickens particular focus on chicken immunology and the are the future!’
13 Other News
The immunology of ageing cell repertoire changes, Natural Killer cells identify selected cell populations of interest. and immunosurveillance, cytomegalovirus The effect of ageing on dynamic activity of and coronary heart disease, antigen sampling these cell populations and trafficking of antigen in the mucosal immune system, age-related necessary for response to pathogen challenge macular degeneration, NFκB dysregulation, will then be determined using multiphoton in and regulatory proteolysis. vivo imaging. Mari’s poster was titled, “Functional cellular An improved understanding of how ageing imaging of immune responses during ageing affects functional activity in the immune system using intravital multiphoton microscopy”. may reveal novel targets for intervention to The immune system protects individuals alleviate age-related immune dysfunction and Mari and Neil against bacteria, viruses and other pathogens, possibly lighten the medical burden of ageing. and if compromised can result in susceptibility ari. Pattison, a PhD student in Dr Neil to infection. Immunosenescence refers to MMabbott’s laboratory won the poster prize age-related immune impairments in immune at February’s Euroscicon event: in London. function that may contribute to increased Immunological changes which occur prevalence and severity of infectious disease during ageing, are thought to contribute to in the elderly. an increase in susceptibility to infectious Despite increasing understanding of molecular diseases and inflammation and a decrease and cellular age-related immune alterations, in responsiveness to vaccines, among the knowledge is incomplete, particularly on elderly. Our increasingly elderly population dynamic and functional cellular changes. with its associated morbidity, underlies the Mari is employing new multiphoton need to understand how the effects of ageing microscopy methods to investigate the can be delayed or limited structural and cellular changes in lymphoid This meeting, organised by Euroscicon, was tissues during ageing, with focus given to the first event of the 2014 Ageing Summit. imaging the dynamic interactions between Other events included Biomarkers and Ageing antigen and lymphoid cells that influence and Establishing Anti-Ageing Medicines. antigen trapping, trafficking and effector The meeting was chaired by Neil Mabbott responses. from The Roslin Institute and Dr Milica Initial studies in mice of different ages Vukmanovic-Stejic, from UCL Medical School, have used immunohistochemistry and flow London. This most interesting meeting cytometry to define key structural and cellular covered a wide range of topics including; B changes. This information will be used to
14 Career Development Committee Report of Edinburgh report “Tapping all our talents” Sang, Tom Freeman and Colin Farquharson http://goo.gl/Fd5pJ. Interconnect Whilst the Career Development Committee Roslin hosted a “Research Insight” visit for develops support for ALL early career Scottish female undergraduates through researchers, it also considers opportunities INTERCONNECT (http://www.interconnect. for positive action to assist underrepresented org.uk/) and the Scottish Resource Centre for groups in science. Change is happening, Women on 24th March 2014. Interconnect but change comes slowly and our aim is provides a network for women studying to increase the speed of that change and science, engineering, technology and the built encourage access and participation, so that environment in Scotland and brings together everyone has the same opportunities within students, qualified women and employers. he Career Development Committee has the Institute. The students were welcomed by Liz Glass Tbeen busy completing The Roslin Institute’s and were given a guided tour of the building application for an Athena SWAN silver award. by Tricia Hart. There were talks from a range Athena SWAN is a government-sponsored of women scientists at the Institute including charter for women in science aimed at advancing Natalia Grundwald (PhD student), Abigail the representation of women in science, Diack (postdoctoral fellow), Pip Beard (Career technology, engineering, medicine and maths Track Fellow) and Simone Meddle (Group (STEMM) www.athenaswan.org.uk. Leader) who described what it is really like A silver award requires us to demonstrate that to be at these various stages in their careers. we have established policies and made changes The students were a very lively bunch and in response to staff surveys etc. that support interacted enthusiastically. They also enjoyed female scientists in developing their careers. The story at Roslin… a networking lunch with women staff and We are sometimes challenged because we The Institute has ~55% female scientists students from The Roslin Institute. They provide some career development activities at postdoctoral level, dropping to 28% at clearly enjoyed their day here, with feedback that are only for female staff and students. professorial level. The proportion of female including:‘wonderful experience. I now know These are often provided by the Scottish professors is gradually increasing and is that I want to do a PhD in the future’ ‘It was Resource Centre for Women in STEMM higher than the UK average in biosciences really helpful to see how successful women in http://goo.gl/BZ1bXq, a centre funded by (~15%) but as stated above we need to keep science achieved their goals in terms of career the Scottish Government to increase the pushing for change by providing a supportive choices and decisions. I felt very inspired and participation of women in STEMM education and inclusive environment for all staff. Our aim motivated’ ‘I am now more confident about and employment in Scotland. is for this to be recognised with an Athena myself and I’ll not be scared to contact other While the days of overt discrimination SWAN gold award in the next few years. people’ ‘Very useful! I was not aware of a lot against women in science are past, women As already mentioned, many of the activities of the things discussed today. Enjoyed asking are still leaving the field in higher numbers we are developing are aimed at supporting questions to the speakers. Excellent!’ than their male colleagues. Over half of staff irrespective of gender. For example: Career development support the UK biosciences undergraduates are Extra Childcare Fees for attending The CDC is planning to develop short female (62%), but that drops to 15% at Scientific Conferences seminars with presentations from people with professorial level. Research and publications We are piloting a small grant fund to support science backgrounds, describing how their analysing the causes of this disparity indicate the extra costs incurred by parents of young careers have developed since they obtained a that the influence of unconscious bias in children when they attend conferences. PhD, covering a wide range of careers. These appointments, promotions, grant awards and Grant writing course will be developed with the Postdoc Society publication success has a negative effect on In March, Mark Stevens, Paul Digard and (chair Louise Stephen) and after discussion career advancement for female scientists. Helen Sang ran a grant-writing course for with the PhD student liaison committee. Other issues, for example relative lack of role postdocs and career track fellows, covering If you want to follow up on any items in this models and support for working mothers, are the whole process of putting a grant together article or have suggestions for future activities also thought to inhibit women from pursuing and understanding the assessment process. supported by the CDC contact Cat Eastwood a scientific career. The story is the same This was well received and will be run again [email protected] or nationally across all other scientific subjects. later this year. Helen Sang [email protected] or For all science, technology, engineering and Fellowship Application Support Group any member of the committee. The current maths (STEM) subjects, the gender gaps We also now have a fellowship application committee members are: Ross Houston, widens every step of the way ©A Chemical support (FAS) group that will offer support, Pam Wiener, Natalia Grundwald, Jeanette Imbalance/Marie Lidén and Siri Rødnes from reviewing applications to mock interviews, Johansson, Louise Stephen, Bruce Whitelaw, http://goo.gl/h4tvCg. A lot more information to anyone applying for a fellowship. Members Liz Glass, John Hopkins, Adam Balic. and data are presented in the Royal Society of the FAS Group include: David Gally, Helen
15 Roslin and Dick Vet staff head for India
Professor Whitelaw said of the conference, “This event provides the Edinburgh team with an excellent opportunity to meet colleagues Bruce from India to discuss ways that we can learn from each other and share research and veterinary expertise to improve animal health.” nimal health and welfare experts from The conference was a collaborative event The Roslin Institute and Royal (Dick) A between the University of Edinburgh’s Royal School of Veterinary Studies (R(D)SVS) at (Dick) School of Veterinary Studies and the University of Edinburgh recently took part the Commonwealth Veterinary Association; in the international “Advances in Veterinary Karnataka Veterinary, Animal and Fisheries Research: Impact and Opportunities” Sciences University, Bidar; and the National conference in Bangalore. Institute of Animal Nutrition and Physiology, The five-day event, of which the University Bangalore. of Edinburgh was one of the organisers, Professor Sir Timothy O’Shea, Principal of looked at ways to improve the quality of life the University of Edinburgh, said, “India is for livestock and pets, as well as diseases a tremendously important country for the threatening India’s wildlife. University of Edinburgh. The quality of the Topics included new techniques to address students who apply to us is very high and infectious diseases in herd animals and the numbers wishing to study at Edinburgh examining treatments for zoonotic diseases – continues to rise. This conference [allowed] those affecting both humans and animals. discussion of crucially important issues The conference included a public lecture that affect India and the wider world and I on the subject of genetically-engineered look forward to valuable partnerships being livestock. Professor Bruce Whitelaw from established between Edinburgh and our The Roslin Institute undertakes research friends in India.” that considers the opportunities that genetic engineering offers the sector in improving the health and welfare of livestock.
16 A celebration of Tony Nash’s career
he Roslin Institute recently had the very great making bodies and latterly he was a member of for MicroarrayBernadette Technology Dutia (£2.4M) bringing this Tprivilege to celebrate the career of Professor the BBSRC Council for four years. new technology to the University of Edinburgh as Tony Nash, who retired at the beginning of March Tony was Editor-in-Chief of the Journal of it was being developed. having been one of our researchers since 2008. General Virology from 1989-1996 and was a In 2007 Tony Nash led a consortium of scientists Tony was appointed to the Chair of Veterinary Society for General Microbiology Council Member from the Universities of Edinburgh, St Andrews Pathology in the Royal (Dick) School of Veterinary 2000-2004. He has served on the editorial and Glasgow to establish the Interdisciplinary Studies (R(D)SVS) in 1994 and was head of the boards of numerous national and international Centre for Human and Avian Influenza Research Department of Veterinary Pathology from 1994 journals including Journal of General Virology, (ICHAIR). ICHAIR brought together basic virology until reorganisation in 2004. During this period Immunology, Viral Immunology and Journal of and influenza virus pathogenesis studies with he led the Laboratory for Clinical and Molecular Neurovirology. mathematical modelling and epidemiology by Virology and promoted the strategy that brought In addition to his commitment to the scientific way of a series of cross-disciplinary workshops together the University of Edinburgh’s research research, Tony has contributed significantly and PhD studentships. This directly resulted in in virology under one roof. to teaching within the University of Edinburgh publications in major journals as well as patents An ongoing theme of his contribution to the and has been an active and enthusiastic identifying novel anti-viral therapies that hold University and its reputation has been to undergraduate teacher seeking to inspire promise for future prophylaxis and treatment of encourage interactions between scientists and students by exposing them to the latest research. influenza virus infections. enhance interdisciplinary research. He was He was instrumental in setting up the BSc (Hons) On the 26th March Bernadette Dutia, Paul appointed Director of the cross-College Centre in Virology and has continued to contribute to Digard and Bob Dalziel organised a symposium for Infectious Disease working enthusiastically to teaching in Infectious Diseases Honours. He has and a dinner to celebrate Tony’s career. It bring together individuals from various campuses supervised numerous postgraduate students was a wonderful afternoon that provided an with interests in infectious diseases. His vision who, inspired by Tony, are now active in research opportunity for many of Tony’s long term friends included bringing together social scientists, across the world. and collaborators to offer their own tributes and clinicians and basic scientists and he organised Throughout his career, Tony has been an present some of the science that Tony has had numerous interactive meetings bringing together innovative scientist actively embracing new such a great influence on over the years. individuals whose paths did not normally cross ideas and technologies and promoting cross- Tony Nash’s contribution as an ambassador of to encourage collaboration. In doing so, he disciplinary interests. Perhaps one of his most The Roslin Institute, R(D)SVS and the University broadened the horizons of many in the University important contributions to international science of Edinburgh has been outstanding in both a and thus laid the foundations for the formation of was his recognition of the potential importance national and international forum. He has been an Edinburgh Infectious Diseases which continues of a group of murine herpesviruses isolated in innovative and inspiring scientist and a wonderful his work in this area. Czechoslovakia and his development of the teacher. He has left a considerable and lasting On a National Level, Tony was a supreme murine model of gammaherpesvirus infection. legacy to the field of infectious diseases. ambassador for the University making major This is now used worldwide as the only available We at The Roslin Institute wish him well for his contributions to UK science nationally. From natural model for human gammaherpesvirus retirement although we are happy to note that 1995-2005 he was an active member of a diseases. Tony co-led the application of the he will continue to be a key contributor to the number of BBSRC and MRC panels and decision Scottish Funding Council to set up the Centre Institute’s research for some time yet. 17 Bernadette Dutia Bob Dalziel
Stacey Efstathiou
John Fazakerley
Seph Borrow
Family Nash 18 Open labs event
uring National Science Week this March this was the first coordinated national effort to students about how they felt and what they DThe Roslin Institute was excited to work allow children to see first-hand what happens expected to see. The responses indicated that with Understanding Animal Research on the in research facilities, and help them to make the students were largely “excited”, “nervous” first of their ‘Open Labs’ events. up their own minds about this controversial yet and “interested” about seeing animals with This year’s Open Labs was the start of a important subject. unusual traits. In post event feedback we three-year programme during which hundreds After a talk about the use of animals in were reminded by comments such as “[I was] of school children will tour research facilities, research by The Roslin Institute’s named vet, surprised by how big cows are” that most meet scientists and veterinarians, and see Lesley Penny, 40 students from Beeslack children don’t get to see livestock species up animals at the centre of research. High School, Penicuik High School, Stewart’s close. More detailed feedback from students Open Labs has been developed by Melville College and Kirkcaldy High School noted that the visit “gave students a much Understanding Animal Research, an visited The Roslin Institute’s poultry facility better understanding of research using educational charity that aims to achieve broad and farm where they met scientists and animals, ethical and practical concerns” and understanding and acceptance of the humane the technical support staff who work in the “It [was a] unique experience and tells you use of animals in biomedical research in the facilities. Adrian Sherman and John Bracken a lot about the advancement being made in UK (www.understandinganimalresearch.org. provided the tours and explained the kind of science … undoubtedly an experience of a uk). The Open Labs event was organised in work that happens before answering questions lifetime” (respectively Laura Lim and Shuuam collaboration with The Roslin Institute and eight about their research and their experiences of Sarkar, both Stewart’s Melville College) other leading science centres across the UK. working with animals. For more about the Open Labs event, please visit: Understanding Animal Research already Before going into the facilities Nicola Stock, http://www.understandinganimalresearch.org. conducts over 300 schools talks every year but Public Engagement Officer had asked the uk/news/2014/03/open-labs-/
19 it can do for you and what The Genetics Society
Iveta
By Doug Vernimmen, The Roslin Institute’s representative at the Genetics Society he Genetics Society acts to support and students are also eligible to apply only for the Tpromote research and teaching of genetics Society’s meetings. However, PhD students in the UK. It covers the study of genomes, are eligible for Heredity Fieldwork Grants genes and gene action, and embraces scales to cover expenses involved in pursuing a ranging from the molecular and cellular to the genetic-based fieldwork project. Annual population and ecosystem level. workshops targeting budding geneticists are The Society’s members come both from the also hosted by the Society. UK and overseas and it maintains links with The Genetics Society awards a number several genetics societies in other countries. of named Medals and Prize Lectures to Membership is open to anyone with an interest outstanding and distinguished geneticists each in genetics research or teaching. The Society year. Additionally, the Sir Kenneth Mather runs two scientific meetings a year, and Memorial Prize is awarded to a student (BSc, provides funding for specialist interest groups MSc or PhD) for excellence in quantitative or and “one-off” scientific meeting organisation. population genetics. The next Spring Meeting, “Breeding for Bacon, I have recently been appointed as Beer and Biofuels”, will be held at The Roslin Representative for The Roslin Institute Institute on 16-17th April, 2015. and since then ten scientists (mainly PhD The Genetics Society is particularly supportive students) have joined the Genetics Society, of students and encourages meeting and with a few of these already benefiting from workshop attendance by providing funding for grants. Last month, Kay Boulton, the current travel and fees for conferences with a genetic post grad rep of the Society joined The Roslin slant to graduate students and postdoctoral Institute as a postdoc in Pete Kaiser’s group. scientists within two years of their PhD I am, therefore, encouraging other people to viva at the time of the application. Several apply for memberships and hope to see The grant schemes are available to provide Roslin Institute becoming a strong contributor financial support for students interested in in the future. gaining research experience in any area Photo: Iveta Gazova, Chromatin Group (Doug of genetics. Summer Studentships are Vernimmen’s lab) has been awarded £800 for awarded to undergraduates wishing to carry attending to a training course on advanced out a research project over the long vacation, RNA-Seq and ChiP-Seq data analysis at the usually prior to their final year; undergraduate European Bioinformatics Institute (EBI).
20 Roslin scientist gets a buzz from his research here are some scientists who just can’t Nosema ceranae, has been implicated infection with Nosema ceranae and also Tseem to leave their work in the lab. in causing disease and colony failure identify viruses introduced to honey bees Dr Mark Barnett, a postdoc at The Roslin in bees but wasn’t thought to be by varroa. The funding includes purchase Institute, is just such a person. By day Mark widespread in the UK. However, the of 18 field microscopes that were bought studies the transcriptomics of macrophage research showed that Nosema ceranae for beekeepers to identify the presence activation and the cell cycle; by night he is present throughout Scotland so it is of Nosema spores. Suspected positive works as part of a group of enthusiastic now important to monitor its presence samples will be forwarded to SASA labs beekeepers in Scotland, which is helping and association with colony losses. for confirmation by specially trained scientific study of the health of bee ‘Sparking Impact’ funding from the beekeepers. populations across the country as part of BBSRC, awarded to the University of a BBSRC-funded scheme. Dundee, will enable Dr Connolly and As reported in BBSRC’s Business his team to include screening for other magazine (Winter 2014 issue), parasites and map honeybee populations beekeepers have already helped discover in Scotland, with help from Science and the widespread presence of an important Advice for Scottish Agriculture (SASA). bee parasite in the UK for the first time, Given the high failure rate of honeybee and now the scheme is being extended colonies seen in Scotland in 2012-13 it is to paint a detailed picture of bee disease important to investigate whether particular in Scotland. threats correlate with honeybee losses. Mark and the other beekeepers, all This is only possible by collaborating with members of the Scottish Beekeepers beekeepers to provide large scale field data. Association (SBA), have been working The aim of the project is for beekeepers, with Dr Chris Connolly from the University including Mark, to learn molecular and of Dundee to identify possible spores microscopy techniques to diagnose from Nosema ceranae.
Roslin student is PWSA prize winner Tian Chee Lu with Patrick Garland iss Tian Chee Lu a vet student at the oral presentation or poster presentation MRoyal (Dick) School of Veterinary at the meeting. They are awarded to Studies won one of the World Poultry authors who are either undergraduates Science Association’s (WPSA) UK Branch who have completed a study as part of President’s prizes for her presentation their first degree or to postgraduates who “Understanding factors Influencing are continuing studies towards a further antimicrobial activity of Gallin; a member qualification. of the ovodefensin family.” The WPSA UK Branch prides itself on the Tian’s presentation covered research rigour applied during the review process before she undertook at The Roslin Institute as the submissions are allowed to be included in part of a WPSA-funded Summer Vacation the programme and subsequent publication Scholarship. in British Poultry Science Abstracts. The The WPSA UK Branch is a registered standard this year was excellent both in terms charity whose aims are to promote of content and delivery. education and research into poultry Prizes were awarded by the outgoing science and the dissemination of president of the WPSA UK Branch, Mr information to others particularly industry. Patrick Garland, to Tian and Keeley Ellis, The prize was awarded at April’s WPSA Nottingham Trent University. Both winners UK Branch Annual Meeting held at received a framed certificate along with a Nottingham University Jubilee Campus. cheque for £100. The Presidents Prizes are awarded Details of WPSA awards, branch activities to those speakers who are considered and membership can be found at www. to have delivered an outstanding short wpsa-uk.com.
21 We are detective! n April The Roslin Institute, with colleagues from Ithe Royal (Dick) School of Veterinary Studies, once again joined the University of Edinburgh in participating in the Edinburgh International Science Festival. The University took up its usual pitch at the National Museum of Scotland, which is also home to Dolly the Sheep. This year Nicola Stock, Public Engagement All the visiting students (around 200 in all) Officer at The Roslin Institute, set up a workshop very expertly discovered that Salmonella “Farm Detectives” aimed at 7-15 years olds. was the bacteria responsible for the disease The one hour workshop invited participants to outbreak and that it had arrived at the farm identify the cause of a disease outbreak affecting from a particular breeder before spreading to cows on a farm. The students used three neighbouring farms following flooding. different techniques, microscopy, epidemiology All the volunteers from the Institute and vet and biochemistry to work out the cause of the school agreed that the workshops had been outbreak. They also learnt about how easy it is to a great success and we look forward to being spread bacteria or viruses by touch. back at the festival next year. Nicola
The Innovation Awards that got away… r Ross Houston and Professor Steve which have contracted these diseases and Ross DBishop, scientists at the University of identifying the difference between them and Edinburgh’s Roslin Institute, were runners up in the genes of those which did not. Fish with two Innovation competitions earlier this year. gene variants leading to greater resistance The first, the Innovator of the Year Competition can be chosen and bred with one another, is run each year by the Biotechnology and decreasing the likelihood of their offspring Biological Sciences Research Council (BBSRC) contracting diseases. to reflect the breadth of the benefits delivered by The second competition that Steve and Ross BBSRC’s investment in UK bioscience were finalists in was the Scottish Enterprise Ross and Steve were among the three finalists 2014 Life Science Awards. Their nomination shortlisted in the category ‘Commercial Innovator’ came in the “Commercial Collaboration” group for their research establishing a genetic test for for their work with salmon production company disease-resistant Atlantic salmon. Landcatch Natural Selection. Salmon farming contributes around half Steve said of their nomination as finalists in the a billion pounds to the UK economy each competitions, “Though we weren’t successful it Steve year and provides healthy, high quality food. was a real honour to be nominated and we were Worldwide, approximately 1.5 million tonnes delighted to have made it through to the finals.” of Atlantic salmon are produced every year. Ross continued, “These nominations are the Farmed salmon are kept together in freshwater result of a real team effort and we must also tanks, and later in large salt water cages to thank our many collaborators both academic grow. In these environments, diseases caused and industrial for being important contributors by viruses and bacteria can spread, as well to this research.” as outbreaks of sea lice. These factors can Innovator of the Year is one of BBSRC’s devastate the yield of the farm. Fostering Innovation competitions. A full list of Together Ross and Steve have undertaken the finalists along with more information about research aimed at producing salmon which Innovator of the Year can be found at: www. will be less likely to catch diseases or infection. bbsrc.ac.uk/innovator. This is done by looking at the genes of salmon
22 Obituary: Brian McLaughlin by Erol Karacaoglu (Financial Controller) t is with great sadness we report the loss of IBrian Mclaughlin, Account Manager, after an illness bravely borne. Brian joined The Roslin Institute in the year 2000 and quickly became a valued member of the team. He was a gentle man with a good sense of humour, he loved his football, a night out, holidays by the sea, but most of all his family. He will be greatly missed by all of us. Brian loved words and we have just found out he wrote poetry, we are including a poem written by Brian for his mother, we are also including a poem written for Brian by his daughter Sarah from his children. by Brian McLaughlin on the occasion by Sarah McLaughlin; the poem she wrote of his mother’s 76th Birthday, 2004:- for Brian from all of his children:-
he paces up and down the room e sit with a reminiscent state of mind; Just when you thought your football days SSeldom is she at peace WAnd think about all the people you’ve were done, Into the kitchen and back again left behind, Back to the game you went to teach your When will it ever cease? A substantial family tree and a legacy of grandson, four, He’ll even inherit your precious expensive Mind occupied by many thoughts You will always be the father we all adore. rhino, Family matters sore And what he will call it we now all know. Seven followed by fourteen We’ve had many happy childhood A mother to the core memories spent by the sea, When times got hard we hope you kept the Long walks and crashing waves we were peace of mind, She paces up and down the room as happy as could be, That you are the reason we will all keep a Trying to recall With sand on our feet we’d sleep in the close bind, Not bygone days for they are clear back of your car, A true gentleman down to your very core, In fact she remembers them all And you’d be racing to get home to get up Know this now and forever we couldn’t to the bar. love you more. A weekly journey to the chapel Daily to the store Kids grow so fast and soon we’ve all flown Not keen on going very far the nest, She never asked for more But home is where the fire is and where Brian having fun with Brian loved best, colleagues at the 2008 She paces up and down the room Whether it was a pub crawl in Edinburgh Institute ‘it’s a knockout ‘event Thinking of those gone away or in the funny farm, Their thoughts and hopes and loves in life We were proud to be with you as you And what they had to say never lost your charm.
But all the while you walk and think Of times there were before Never doubt that we love you mum And will always find your door
23 Simmonds FRSE rofessor Peter Simmonds from The Roslin of virus infections, and interactions with PInstitute was recently named by the Royal their hosts. This has led to a variety of Society of Edinburgh (RSE) as one of its 53 investigations ranging from evolutionary new Fellows. studies of virus variability and recombination, New Fellows are elected each year by existing molecular epidemiology and studies of viral fellows following a rigorous nomination pathogenesis and interactions of virus with process. host cell defences. This has entailed use and Peter said of his election, “This is certainly an development of a variety of molecular biology honour to be named among some of the great laboratory methods, and a wide range of scientists in Scottish research.” genetic and bioinformatic analysis techniques Peter’s research interest and focus has that have been widely applied in a variety of been in the evolution and epidemiology virus groups.
New award winning start up at Roslin aims to provide you with your own personal journal by James Prendergast ith over 25,000 life science journals and Dipodo just won the University Enterprise W1 million articles published last year Award and is currently up for the national keeping up to date with all the latest research converge challenge so we would love to get in your field can be a challenge. A new start some feedback on how we can improve it up at Roslin aims to change this. Dipodo.com, further to make it even more useful. You can developed by James Prendergast (Genetics find it at www.dipodo.com and any questions, and Genomics), keeps users updated with comments, suggestions can be sent to James relevant research by automatically learning at [email protected]. their interests through examining the articles in their reference collection. Dipodo integrates seamlessly with the main online reference managers, Zotero and Mendeley, and also works with all the major desktop reference managers. Having learnt the type of articles a user reads it identifies all similar articles published each day. No more trawling through journal tables of content or running regular searches. To enable users to more quickly scan through suggested papers, Dipodo also identifies the key sentence from article abstracts so that a user can more quickly see if it is worth digging into the article further. Dipodo also finds blog entries and jobs that match a user’s background, with conferences and funding opportunities being added in the near future. Other features include the ability to find peers with similar interests around the globe, comment on articles, see what others are saying, and easily share interesting papers James with colleagues.
24 Spreading the epigenetic word n recent years, great progress has been Imade in the identification of the molecular players involved in the epigenetic control of gene expression during development. The work of many laboratories has established that regulating the interplay of transcription factors with chromatin components is the major driver of cellular differentiation. Because of their single cell nature and ease of purification, much of what we have learnt about these processes in animals has been Doug delivered based on cellular models within the hematopoietic system. The blood cell describes the intricate processes involved system evolved from a few simple cell types in the development of blood cells across a in more primitive organisms that provide range of organisms from drosophila and fish oxygen transport and carry out phagocytosis at one end, and mammals at the other end. into the complex hematopoietic system of The book, “Transcriptional and Epigenetic mammals, containing many specialized cells Mechanisms Regulating Normal and types with vastly different functions, such as Aberrant Blood Cell Development”, B cells, T cells, granulocytes, macrophages, contains individual chapters devoted erythrocytes, and megakaryocytes. to describing the epigenetic and Doug Vernimmen, a Chancellor’s Fellow at transcriptional mechanisms regulating The Roslin Institute, is one of the contributing hematopoiesis in the different organisms authors for a new book that and orchestrating the differentiation of a wide variety of cell types. Different chapters describe the function of lymphocytes, macrophages and red blood cells and the molecular players, i.e. transcription factors and the epigenetic regulatory machinery driving their differentiation. Most importantly, the book not only describes normal processes, such as the rearrangements of antigen receptor genes, and the regulation of genes by various mechanisms such as DNA methylation, but also outlines what happens when these processes function abnormally to precipitate diseases such as leukemia and immune disorders.
25 R(D)SVS Research Student Day 2014
by Robin Cassady-Cain (above with a couple of potential researchers)
ednesday April 30th saw the institute Whost its annual Student Research Day, where postgraduate students across the campus have the opportunity to showcase their research. The Masters’ candidates and lower year PhD candidates present posters, with the final year students participating in a full day of short talks. As usual, the quality of the research presented was very high and this was reflected in the excellent oral presentations given by the final year PhD candidates. As ever, it was a fascinating window into the breadth of research across the campus, with talks ranging from basic function and mechanistic insights encompassing developmental biology, infection and immunity and neurobiology, to more applied insights related to vaccine development, clinical research and diagnostic development. On a lighter note, my favourite bit of trivia for the day—apparently you can buy emu eggs on ebay! The diverse day of research talks finished off with the Charnock Bradley Lecture, delivered by Prof. Os Jarrett from the University of Glasgow, who gave a lively historical overview on the basic and translational insights from his (and others) research on feline leukaemia virus. The day was rounded out by a drinks and nibbles reception, where the prizes for the posters and talks were awarded (and I didn’t envy the judges the difficulty of that task!).
26 27 The prizes were as follows: MSc: Emma Hurst Clinical Scholar: 1st prize Declan King, Anuj Seghal, Smaragda Congratulations to all the winners. Thanks to Valentina Palermo, Runner up Justine Tsairidou all the students as a group for their high quality presentations on the day, and reminding us all Kane-Smith rd st 3 year PhD: 1 prize Will Ho, Runners up: of the diverse and interesting research that is 1st year PhD: 1st prize Rodrigo Bacigalupe, Amy Richards, Ruth Morgan occurring across the campus. Runners up: Janice Kwok, Laura Graham Talks: 1st prize Breno Beirão, Runners 2nd year PhD: winners: Jason Ioannidis, up: Ed Johnson, Amr Bayoumy, Special Mention: Scott Kilpatrick, Alex Corbishley
28 29 eter Hohenstein and Bruce Whitelaw of The Roslin Institute are involved in the organization of the 12th Transgenic PTechnology Meeting, which is going to take place in Edinburgh, October 2014.
More information here:
30 Recent Roslin Institute Publications
Abdelbary, M.M.H., Wittenberg, dinucleotide frequencies on RNA and cellular hypoxia. Laryngoscope A., Cuny, C., Layer, F., Kurt, K., virus replication and characterization E-pub 5 April,DOI: 10.1002/ Wieler, L.H., Walther, B., Skov, R., of the innate cellular pathways lary.24698. Larsen, J., Hasman, H., Fitzgerald, underlying virus attenuation and J.R., Smith, T.C., Wagenaar, J.A., enhanced replication. Nucleic Acids Biffa, D., Johansen, T.B., Godfroid, Pantosti, A., Hallin, M., Struelens, Research 42(7), 4527-4545,DOI: J., Muwonge, A., Skjerve, E. M.J., Edwards, G., Böse, R., Nübel, 10.1093/nar/gku075. and Djønne, B. (2014) Multi- U. and Witte, W. (2014) Phylogenetic locus variable-number tandem Analysis of Staphylococcus aureus Bailey, I.E., Morgan, K.V., Bertin, M., repeat analysis (MLVA) reveals CC398 Reveals a Sub-Lineage Meddle, S.L. and Healy, S.D. (2014) heterogeneity of Mycobacterium Epidemiologically Associated with Physical cognition: Birds learn the bovis strains and multiple Infections in Horses. PLoS One structural efficacy of nest material. genotype infections of cattle in 9(2), e88083, DOI: 10.1371/journal. Proceedings of the Royal Society B: Ethiopia. Infection, Genetics and pone.0088083. Biological Sciences 281(1784),DOI: Evolution 23, 13-19,DOI: 10.1016/j. 10.1098/rspb.2013.3225 meegid.2014.01.021. Abdollahi-Arpanahi, R., Nejati- Javaremi, A., Pakdel, A., Moradi- Barakzai, S.Z. and Dixon, P.M. Bouwman, A.C., Hickey, J.M., Shahrbabak, M., Morota, G., (2014) Equine Standing Sinus Calus, M.P. and Veerkamp, R.F. Valente, B.D., Kranis, A., Rosa, Surgery. Veterinary Clinics of (2014) Imputation of non-genotyped G.J.M. and Gianola, D. (2014) North America - Equine Practice individuals based on genotyped Effect of allele frequencies, effect 30(1), 45-62,DOI: 10.1016/j. relatives: assessing the imputation sizes and number of markers on cveq.2013.11.004. accuracy of a real case scenario in prediction of quantitative traits dairy cattle. Genet Sel Evol 46(1), in chickens. Journal of Animal Barakzai, S.Z., Dixon, P.M., 6,DOI: 10.1186/1297-9686-46-6. Breeding and Genetics 131(2), 123- Hawkes, C.S., Cox, A. and Barnett, 133, DOI: 10.1111/jbg.12075. T.P. (2014) Upper Esophageal Bradford, B., Crocker, P.R. and Incompetence in Five Horses After Mabbott, N. (2014) Peripheral prion Abdollahi-Arpanahi, R., Pakdel, Prosthetic Laryngoplasty. Veterinary disease pathogenesis is unaltered A., Nejati-Javaremi, A., Moradi Surgery E-pub 31 January,DOI: in the absence of sialoadhesin Shahrbabak, M., Morota, G., 10.1111/j.1532-950X.2014.12101.x. (Siglec-1/CD169). Immunology Valente, B.D., Kranis, A., Rosa, E-pub 28 March,DOI: 10.1111/ G.J.M. and Gianola, D. (2014) Barria, M.A., Balachandran, A., imm.12294. Dissection of additive genetic Morita, M., Kitamoto, T., Barron, R., variability for quantitative traits Manson, J., Knight, R., Ironside, J.W. Bradford, B., Piccardo, P., Ironside, in chickens using SNP markers. and Head, M.W. (2014) Molecular J. and Mabbott, N. (2014) Human Journal of Animal Breeding and barriers to zoonotic transmission of prion diseases and the risk of their Genetics E-pub 25 January,DOI: prions. Emerg Infect Dis 20(1), 88- transmission during anatomical 10.1111/jbg.12079. 97,DOI: 10.3201/eid2001.130858. dissection. Clinical Anatomy E-pub 17 April,DOI: 10.1002/ca.22403. Alton, E.W., Boyd, A.C., Cheng, Beale, R., Wise, H., Stuart, S.H., Davies, J.C., Davies, L.A., A., Ravenhill, B.J., Digard, P. Brunton, P.J., Russell, J.A. and Dayan, A., Gill, D.R., Griesenbach, and Randow, F. (2014) A LC3- Hirst, J.J. (2014) Allopregnanolone U., Higgins, T., Hyde, S.C., Innes, Interacting Motif in the Influenza in the brain: protecting pregnancy J.A., McLachlan, G., Porteous, A Virus M2 Protein Is Required to and birth outcomes. Prog Neurobiol D., Pringle, I., Scheule, R.K. and Subvert Autophagy and Maintain 113(S1), 106-136,DOI: DOI: Sumner-Jones, S. (2014) Toxicology Virion Stability. Cell Host Microbe 10.1016/j.pneurobio.2013.08.005. study assessing efficacy and safety 15(2), 239-247,DOI: 10.1016/j. of repeated administration of lipid/ chom.2014.01.006. Busin, V., Kenyon, F., Parkin, T., DNA complexes to mouse lung. McBean, D., Laing, N., Sargison, Gene Therapy 21(1), 89-95,DOI: Bermingham, M.L., Bishop, S.C., N.D. and Ellis, K. (2014) Production 10.1038/gt.2013.61. Woolliams, J.A., Pong-Wong, R., impact of a targeted selective Allen, A.R., McBride, S.H., Ryder, treatment system based on Andersson, R., Gebhard, C., Miguel- J.J., Wright, D.M., Skuce, R.A., liveweight gain in a commercial flock. Escalada, I., Hoof, I., Bornholdt, J., McDowell, S.W. and Glass, E.J. Veterinary Journal 200(2), 248- Boyd, M., K.J., ….. Summers, K.M., (2014) Genome-wide association 252,DOI: 10.1016/j.tvjl.2014.02.012. Hide, W., Freeman, T.C., et al. study identifies novel loci (2014) An atlas of active enhancers associated with resistance to bovine Busin, V. and Sargison, N.D. (2014) across human cell types and tissues. tuberculosis. Heredity 112(5), 543- Preliminary observations on the value Nature 507(7493), 455-461, DOI: 551,DOI: 10.1038/hdy.2013.137. of using effective anthelmintic drugs to 10.1038/nature12787. control nematode parasitism in lambs Bhutta, M.F., Cheeseman, M.T. and in the face of a high level of infective Atkinson, N.J., Witteveldt, J., Evans, Brown, S.D. (2014) Myringotomy in larval challenge. Small Ruminant D.J. and Simmonds, P. (2014) the Junbo mouse model of chronic Research 119(1-3), 172-175,DOI: The influence of CpG and UpA otitis media alleviates inflammation 10.1016/j.smallrumres.2014.02.001.
31 Cabrerizo, M., Trallero, G. and Webster, R.G. and Taylor, G.L. Genetics 10(5), e1004359,DOI: Simmonds, P. (2014) Recombination (2014) Prevention of influenza 10.1371/journal.pgen.1004359. and evolutionary dynamics of human by targeting host receptors using echovirus 6. Journal of Medical engineered proteins. Proceedings of Crowther, E., Hughes, K., Handel, I., Virology 86(5), 857-864,DOI: DOI: the National Academy of Sciences Whittington, R., Pryce, M., Warman, 10.1002/jmv.23741. 111(17), 6401-6406,DOI: 10.1073/ S., Rhind, S. and Baillie, S. (2014) pnas.1404205111. Stakeholder consultation on Cantón, G.J., Katzer, F., Maley, tracking in UK veterinary degrees: S.W., Bartley, P.M., Benavides- Connelley, T.K., Longhi, C., Burrells, part 1. Veterinary Record E-pub 16 Silván, J., Palarea-Albaladejo, J., A., Degnan, K., Hope, J., Allan, A.J., May,DOI: 10.1136/vr.102342. Pang, Y., Smith, S.H., Rocchi, M.S., Hammond, J.A., Storset, A.K. and Buxton, D., Innes, E.A. and Chianini, Morrison, W.I. (2014) NKp46+CD3+ Crowther, E., Hughes, K., Handel, I., F. (2014) Inflammatory infiltration Cells: A Novel Nonconventional Whittington, R., Pryce, M., Warman, into placentas of Neospora caninum T Cell Subset in Cattle Exhibiting S., Rhind, S. and Baillie, S. (2014) challenged cattle correlates with Both NK Cell and T Cell Features. Stakeholder consultation on clinical outcome of pregnancy. Journal of Immunology 192(8), tracking in UK veterinary degrees: Veterinary Research 45(1), 11,DOI: 3868-3880,DOI:10.4049/ part 2. Veterinary Record E-pub 12 10.1186/1297-9716-45-11. jimmunol.1302464. May,DOI: 10.1136/vr.102341. Cappellini, E., Gentry, A., Conradie van Wyk, I., Goddard, Davey, M.G., McTeir, L., Barrie, Palkopoulou, E., Ishida, Y., Cram, A.D., Bronsvoort, M., Coetzer, A.M., Freem, L.J. and Stephen, D., Roos, A.M., Watson, M., J.A.W., Handel, I., Hanotte, O., L.A. (2014) Loss of cilia causes Johansson, U.S., Fernholm, B., Jennings, A., Lesosky, M., Kiara, embryonic lung hypoplasia, liver Agnelli, P., Barbagli, F., Littlewood, H., Poole, J., Thumbi, S., Toye, P., fibrosis, and cholestasis in the talpid D.T.J., Kelstrup, C.D., Olsen, J.V., Woolhouse, M. and Penzhorn, B.L. ciliopathy mutant. Organogenesis Lister, A.M., Roca, A.L., Dalén, (2014) The impact of co-infections 10(2),DOI: 10.4161/org.28819. L. and Gilbert, M.T.P. (2014) on the haematological profile of East Resolution of the type material of the African Short-horn Zebu calves. Davies, G., Harris, S.E., Reynolds, Asian elephant, Elephas maximus Parasitology 141(3), 374-388,DOI: C.A., Payton, A., Knight, H.M., Linnaeus, 1758 (Proboscidea, 10.1017/S0031182013001625 Liewald, D.C., Lopez, L.M., Elephantidae). Zoological Journal of Luciano, M., Gow, A.J., Corley, J., the Linnean Society 170(1), 222-232. Corbin, L.J., Kranis, A., Blott, S.C., Henderson, R., Murray, C., Pattie, Swinburne, J.E., Vaudin, M., Bishop, A., Fox, H.C., Redmond, P., Lutz, Carpanini, S.M., McKie, L., S.C. and Woolliams, J.A. (2014) M.W., Chiba-Falek, O., Linnertz, C., Thomson, D., Wright, A.K., Gordon, The utility of low-density genotyping Saith, S., Haggarty, P., McNeill, G., S.L., Roche, S.L., Handley, for imputation in the Thoroughbred Ke, X., Ollier, W., Horan, M., Roses, M.T., Morrison, H., Brownstein, horse. Genet Sel Evol 46(1), 9,DOI: A.D., Ponting, C.P., Porteous, D.J., D., Wishart, T.M., Cousin, M.A., 10.1186/1297-9686-46-9. Tenesa, A., Pickles, A., Starr, J.M., Gillingwater, T.H., Aligianis, I.A. and Whalley, L.J., Pedersen, N.L., Jackson, I.J. (2014) A novel mouse Cottle, H.J., Hughes, K.J., Pendleton, N., Visscher, P.M. and model of Warburg Micro Syndrome Thompson, H., Johnston, P.E.J. Deary, I.J. (2014) A genome-wide reveals roles for RAB18 in eye and Philbey, A.W. (2014) Primary association study implicates the development and organisation of hyperparathyroidism in a 17-year- APOE locus in nonpathological the neuronal cytoskeleton. Disease old Arab x Welsh Cob pony mare with cognitive ageing. Molecular Models & Mechanisms E-pub 24 a functional parathyroid adenoma. Psychiatry 19(1), 76-87,DOI: DOI: April,DOI: 10.1242/dmm.015222. Equine Veterinary Education E-pub 10.1038/mp.2012.159. 5 May,DOI: 10.1111/eve.12183. Cebamanos, L., Gray, A., Stewart, Davies, L.A., Nunez-Alonso, G.A., I. and Tenesa, A. (2014) Regional Craig, N.M., Smith, D.W., Pate, McLachlan, G., Hyde, S.C. and Heritability Advanced Complex Trait J.A., Morrison, I.W. and Knight, P.A. Gill, D.R. (2014) Aerosol delivery Analysis for GPU and Traditional (2014) Local cytokine transcription in of DNA/liposomes to the lung for Parallel Architectures. Bioinformatics naïve and previously infected sheep cystic fibrosis gene therapy. Human 30(8), 1177-1179,DOI: 10.1093/ and lambs following challenge with gene therapy. Clinical development bioinformatics/btt754. teladorsagia circumcincta. BMC E-pub 29 April,DOI: 10.1089/ Veterinary Research 10(Article hum.2014.019. Chrzastek, K., Borowska, D., Kaiser, 87),DOI: 10.1186/1746-6148-10-87. P. and Vervelde, L. (2014) Class B Diack, A.B., Head, M.W., CpG ODN stimulation upregulates Cross, S.H., Macalinao, D.G., McCutcheon, S., Boyle, A., Knight, expression of TLR21 and IFN-ϒ McKie, L., Rose, L., Kearney, A.L., R., Ironside, J.W., Manson, J.C. and in chicken Harderian gland cells. Rainger, J., Thaung, C., Keighren, Will, R.G. (2014) Variant CJD: 18 Veterinary Immunology and M., Jadeja, S., West, K., Kneeland, years of research and surveillance. Immunopathology E-pub 6 May,DOI: S.C., Smith, R.S., Howell, G.R., Prion 8(3). 10.1016/j.vetimm.2014.04.010. Young, F., Robertson, M., van T’ Hof, R., John, S.W. and Jackson, Dixon, P.M., Ceen, S., Barnett, Connaris, H., Govorkova, E.A., I.J. (2014) A Dominant-Negative T., O’Leary, J.M., Parkin, T. and Ligertwood, Y., Dutia, B.M., Yang, Mutation of Mouse Lmx1b Causes Barakzai, S. (2014) A long-term L., Tauber, S., Taylor, M.A., Glaucoma and Is Semi-lethal via study on the clinical effects of Alias, N., Hagan, R., Nash, A.A., LBD1-Mediated Dimerisation. PLoS mechanical widening of cheek
32 teeth diastemata for treatment of occidentalis. Ardea 101(2), 165- Meng, X.J. and Opriessnig, T. periodontitis in 202 horses (2008- 170,DOI: 10.5253/078.101.0211 (2014) Comparison of commercial 2011). Equine Vet J 46(1), 76- enzyme-linked immunosorbent 80,DOI: 10.1111/evj.12085. Fitzgerald, J.R. (2014) Evolution assays and fluorescent microbead of Staphylococcus aureus during immunoassays for detection Dixon, P.M., Loh, N. and Barakzai, human colonization and infection. of antibodies against porcine S.Z. (2014) Swellings of the angle Infection, Genetics and Evolution reproductive and respiratory of the mandible in 32 horses 21, 542-547,DOI: 10.1016/j. syndrome virus in boars. J Virol (1997-2011). Veterinary Journal meegid.2013.04.020. Methods 197, 63-66,DOI: 10.1016/j. 199(1), 97-102,DOI: 10.1016/j. jviromet.2013.12.001. tvjl.2013.10.015. Foster, J.D., Goldmann, W. and Hunter, N. (2014) Correction: Gerber, P.F., Xiao, C.T., Cao, D., Dixon, P.M., Savill, D., Horbyl, Evidence in Sheep for Pre-Natal Meng, X.J. and Opriessnig, T. (2014) A., Reardon, R.J. and Liuti, T. Transmission of Scrapie to Lambs Comparison of real-time reverse (2014) Critical evaluation of ex from Infected Mothers. PLoS One transcriptase (RT)-PCR assays for vivo restoration of carious equine 9(1),DOI: 10.1371/annotation/ detection of swine hepatitis E virus maxillary cheek teeth infundibulae f7240569-aa33-4a9c-b7f0- in fecal samples. J Clin Microbiol following high-pressure gas and 75be4fad9999. 52(4), 1045-1051,DOI: 10.1128/ micro-particle abrasion. Veterinary jcm.03118-13. Journal 200(3), 368–374,DOI: Fraga-Manteiga, E., Shaw, D.J., 10.1016/j.tvjl.2014.04.004. Dennison, S., Brownlow, A. and Gill, A.C. (2014) beta-Hairpin- Schwarz, T. (2014) An optimized Mediated Formation of Structurally Ekine, C.C., Rowe, S.J., Bishop, computed tomography protocol Distinct Multimers of Neurotoxic S.C. and de Koning, D.J. (2014) for metallic gunshot head trauma Prion Peptides. PLoS One 9(1), Why breeding values estimated in a seal model. Veterinary e87354,DOI: 10.1371/journal. using familial data should not be Radiology and Ultrasound E-pub 12 pone.0087354. used for genome-wide association February,DOI: 10.1111/vru.12146. studies. G3: Genes, Genomes, Giménez-Lirola, L.G., Gerber, Genetics 4(2), 341-347,DOI: Froydenlund, T.J. and Dixon, P.F., Rowland, R.R., Halbur, P.G., 10.1534/g3.113.008706. P.M. (2014) A review of equine Huang, Y.W., Meng, X.J. and laryngoplasty complications. Equine Opriessnig, T. (2014) Development Elders, R.C., Holder, A., Smith, Veterinary Education 26(2), 98- and validation of a 4-plex antibody K.C., Baines, S.J. and Catchpole, 106,DOI: 10.1111/eve.12131. assay for simultaneous detection of B. (2014) Recombinant canine IgG antibodies against Torque teno IgE Fc and an IgE Fc-TRAIL Fuller, H.R., Hurtado, M.L., Wishart, sus virus 1 (TTSuV1), TTSuV2, and fusion protein bind to neoplastic T.M. and Gates, M.A. (2014) The rat porcine reproductive and respiratory canine mast cells. Veterinary striatum responds to nigro-striatal syndrome virus types 1 and 2. Immunology and Immunopathology degeneration via the increased Research in Veterinary Science 159(1-2), 29-40,DOI: 10.1016/j. expression of proteins associated 96(3), 543-550,DOI: 10.1016/j. vetimm.2014.02.018. with growth and regeneration rvsc.2014.02.014. of neuronal circuitry. Proteome Esteves, C.L., Kelly, V., Breton, Science 12(Article 20),DOI: Giménez-Lirola, L.G., Jiang, Y.H., A., Taylor, A.I., West, C.C., 10.1186/1477-5956-12-20. Sun, D., Hoang, H., Yoon, K.J., Donadeu, F.X., Peault, B., Halbur, P.G. and Opriessnig, T. Seckl, J.R. and Chapman, K.E. Gao, Z., Dong, Q., Jiang, Y., (2014) Simultaneous detection (2014) Proinflammatory Cytokine Opriessnig, T., Wang, J., Quan, Y. of antibodies against Apx-toxins Induction of 11beta-Hydroxysteroid and Yang, Z. (2014) ORF4-protein I, II, III and VI in pigs with known Dehydrogenase Type 1 (11beta- deficient PCV2 mutants enhance and unknown Actinobacillus HSD1) in Human Adipocytes Is virus-induced apoptosis and show pleuropneumoniae exposure using Mediated by MEK, C/EBPbeta, and differential expression of mRNAs a multiplexing liquid array platform. NF-kappaB/RelA. Journal of Clinical in vitro. Virus Res 183, 56-62,DOI: Clinical and Vaccine Immunology Endocrinology and Metabolism 10.1016/j.virusres.2014.01.024. 21(1), 85-95,DOI: 10.1128/ 99(1), E160-E168,DOI: 10.1210/ cvi.00451-13. jc.2013-1708. Garcia-Morales, C., Rothwell, L., Moffat, L., Garceau, V., Balic, Gonen, S., Lowe, N.R., Cezard, Fantom Consortium, Riken PMI A., Sang, H.M., Kaiser, P. and T., Gharbi, K., Bishop, S.C. and and CLST (DGT), Taylor, M., Hume, D.A. (2014) Production and Houston, R.D. (2014) Linkage Semple, C., Hume, D., Meynert, A., characterisation of a monoclonal maps of the Atlantic salmon (Salmo Summers, K., Freeman, T., Baillie, antibody that recognises the salar) genome derived from RAD K. and Young, R. (2014) A promoter- chicken CSF1 receptor and confirms sequencing. BMC Genomics 15(1), level mammalian expression atlas. that expression is restricted 166,DOI: 10.1186/1471-2164-15-166. Nature 507(7493), 462-470,DOI: to macrophage-lineage cells. 10.1038/nature13182. Developmental and Comparative González-Iglesias, P., Scortti, Immunology 42(2), 278-285,DOI: M., MacArthur, I., Hapeshi, A., Farrell, L.L. (2014) Examining 10.1016/j.dci.2013.09.011. Rodriguez, H., Prescott, J.F. and the genetic distinctiveness of the Vazquez-Boland, J.A. (2014) Mouse western subspecies of yellow- Gerber, P.F., Gimenez-Lirola, lung infection model to assess billed cuckoo coccyzus americanus L.G., Halbur, P.G., Zhou, L., Rhodococcus equi virulence and
33 vaccine protection. Vet Microbiol Hall, D.P., Maccormick, I.J.C., Hernandez-Suarez, G., Sanabria, E-pub 30 March,DOI: 10.1016/j. Phythian-Adams, A.T., Rzechorzek, M.C., Serrano, M., Herran, O.F., vetmic.2014.03.026. N.M., Hope-Jones, D., Cosens, Perez, J., Plata, J.L., Zabaleta, S., Jackson, S., Bates, M.G.D., J. and Tenesa, A. (2014) Genetic Griffin, D. and Burt, D.W. (2014) All Collier, D.J., Hume, D.A., Freeman, ancestry is associated with colorectal chromosomes great and small: 10 years T., Thompson, A.A.R. and Baillie, adenomas and adenocarcinomas on. Chromosome Research 22(1), J.K. (2014) Network analysis in Latino populations. European 1-6,DOI: 10.1007/s10577-014-9413-0. reveals distinct clinical syndromes Journal of Human Genetics underlying acute mountain sickness. E-pub 12 February,DOI: 10.1038/ Grit, G.H., Devriendt, B., Van PLoS One 9(1), e81229,DOI: ejhg.2013.310. Coppernolle, S., Geurden, T., Hope, 10.1371/journal.pone.0081229. J., Vercruysse, J., Cox, E., Geldhof, Hocking, P.M. (2014) Unexpected P. and Claerebout, E. (2014) Giardia Hall, Z.J., Bertin, M., Bailey, I.E., consequences of genetic duodenalis stimulates partial Meddle, S.L. and Healy, S.D. (2014) selection in broilers and turkeys: maturation of bovine dendritic cells Neural correlates of nesting behavior problems and solutions. Br associated with altered cytokine in zebra finches (Taeniopygia Poult Sci 55(1), 1-12,DOI: secretion and induction of T cell guttata). Behav Brain Res 264, 26- 10.1080/00071668.2014.877692. proliferation. Parasite Immunology 33,DOI: 10.1016/j.bbr.2014.01.043. 36(4), 157-169,DOI: 10.1111/ Hocking, P.M. and Guggenheim, pim.12095. Harvala, H., Calvert, J., Van J.A. (2014) The chick as an Nguyen, D., Clasper, L., Gadsby, animal model of eye disease. Drug Grit, G.H., Van Coppernolle, S., N., Molyneaux, P., Templeton, Discovery Today: Disease Models Devriendt, B., Geurden, T., Dreesen, K., McWilliams Leitch, C. and E-pub 2 May,DOI: 10.1016/j. L., Hope, J., Vercruysse, J., Cox, Simmonds, P. (2014) Comparison ddmod.2014.02.003. E., Geldhof, P. and Claerebout, E. of diagnostic clinical samples (2014) Evaluation of cellular and and environmental sampling for Hohenstein, P. and Hastie, N.D. humoral systemic immune response enterovirus and parechovirus (2014) LINking microRNAs, kidney against Giardia duodenalis infection surveillance in Scotland, 2010 to development, and Wilms tumors. in cattle. Veterinary Parasitology 2012. Eurosurveillance 19(15). Genes & Development 28(9), 923- E-pub 20 March,DOI: 10.1016/j. 925,DOI: 10.1101/gad.242735.114. vetpar.2014.03.012. Harvala, H., Griffiths, M., Solomon, T. and Simmonds, P. (2014) Holmes, A., McAllister, G., McAdam, Guioli, S., Nandi, S., Zhao, D., Distinct systemic and central P.R., Choi, S.H., Girvan, K., Robb, Burgess-Shannon, J., Lovell- nervous system disease patterns A., Edwards, G., Templeton, Badge, R. and Clinton, M. in enterovirus and parechovirus K. and Fitzgerald, J.R. (2014) (2014) Gonadal Asymmetry infected children. Journal of Infection Genome-wide single nucleotide and Sex Determination in Birds. E-pub 22 March,DOI: 10.1016/j. polymorphism-based assay for Sexual Development E-pub 27 jinf.2014.02.017. high-resolution epidemiological February,DOI: 10.1159/000358406. analysis of the methicillin- Harvala, H., Wong, V., Simmonds, P., resistant Staphylococcus aureus Gunn-Moore, D. (2014) Johannessen, I. and Ramalingam, S. hospital clone EMRSA-15. Clinical Feline tuberculosis caused by (2014) Acute viral hepatitis - Should Microbiology and Infection 20(2), Mycobacterium bovis. Vet Rec the current screening strategy O124-O131,DOI: 10.1111/1469- 174(13), 322-323,DOI: 10.1136/ be modified? Journal of Clinical 0691.12328. vr.g2338. Virology 59(3), 184-187,DOI: 10.1016/j.jcv.2014.01.001. Hook, L.M., Grey, F., Grabski, R., Gunn-Moore, D.A. (2014) Feline Tirabassi, R., Doyle, T., Hancock, mycobacterial infections. Veterinary Hemann, M., Beach, N.M., Meng, M., Landais, I., Jeng, S., McWeeney, Journal E-pub 25 February,DOI: X.J., Wang, C., Halbur, P.G. S., Britt, W. and Nelson, J.A. 10.1016/j.tvjl.2014.02.014. and Opriessnig, T. (2014) A live- (2014) Cytomegalovirus miRNAs attenuated and an inactivated Target Secretory Pathway Genes Gutierrez-Gil, B., Arranz, J.J., Pong- chimeric porcine circovirus (PCV)1- to Facilitate Formation of the Wong, R., Garcia-Gamez, E., Kijas, 2 vaccine are both effective at Virion Assembly Compartment and J. and Wiener, P. (2014) Application inducing a humoral immune Reduce Cytokine Secretion. Cell of selection mapping to identify response and reducing PCV2 Host Microbe 15(3), 363-373,DOI: genomic regions associated with viremia and intrauterine infection 10.1016/j.chom.2014.02.004. dairy production in sheep. PLoS in female swine of breeding age. One 9(5), e94623,DOI: 10.1371/ Canadian Journal of Veterinary Hopker, A., Crilly, J.P. and Philbey, journal.pone.0094623. Research 78(1), 8-16. A.W. (2014) A novel tetralogy of congenital cardiac deformities in a Haga, I.R., Pechenick Jowers, T., Hernandez, S.C., Finlayson, H.A., calf. UK Vet: Livestock 19(2),DOI: Griffiths, S.J., Haas, J. and Beard, Ashworth, C.J., Haley, C.S. and 10.12968/live.2013.19.2. P.M. (2014) TRAF2 facilitates Archibald, A.L. (2014) A genome- Vaccinia virus replication by wide linkage analysis for reproductive Houston, R.D., Taggart, J.B., Cézard, promoting rapid virus entry. Journal traits in F2 Large White × Meishan T., Bekaert, M., Lowe, N.R., Downing, of Virology 88(7), 3664-3677,DOI: cross gilts. Animal Genetics 45(2), A., Talbot, R., Bishop, S.C., Archibald, 10.1128/jvi.03013-13. 191-197,DOI: 10.1111/age.12123. A.L., Bron, J.E., Penman, D.J., Davassi, A., Brew, F., Tinch, A.E., Gharbi, K.
34 and Hamilton, A. (2014) Development Jones, M., Dry, I.R., Frampton, s1751731113001717. and validation of a high density SNP D., Singh, M., Kanda, R.K., Yee, genotyping array for Atlantic salmon M.B., Kellam, P., Hollinshead, M., Kowalczyk-Quintas, C., Willen, (Salmo salar). BMC Genomics 15(1), Kinchington, P.R., O’Toole, E.A. L., Dang, A.T., Sarrasin, H., Article 90,DOI: 10.1186/1471-2164-15- and Breuer, J. (2014) RNA-seq Tardivel, A., Hermes, K., 90. Analysis of Host and Viral Gene Schneider, H., Gaide, O., Donze, Expression Highlights Interaction O., Kirby, N., Headon, D.J. and Intarak, N., Muangsombut, V., between Varicella Zoster Virus and Schneider, P. (2014) Generation Vattanaviboon, P., Stevens, M.P. Keratinocyte Differentiation. PLoS and Characterization of Function and Korbsrisate, S. (2014) Growth, Pathogens 10(1), e1003896,DOI: Blocking Anti-Ectodysplasin A motility and resistance to oxidative 10.1371/journal.ppat.1003896. (EDA) Monoclonal Antibodies that stress of the melioidosis pathogen Induce Ectodermal Dysplasia. Burkholderia pseudomallei Kasioulis, I., Syred, H.M., Tate, P., Journal of Biological Chemistry are enhanced by epinephrine. Finch, A., Shaw, J., Seawright, A., 289(7), 4273-4285,DOI: 10.1074/ Pathogens and disease E-pub Fuszard, M., Botting, C.H., Shirran, jbc.M113.535740. 12 May,DOI: 10.1111/2049- S., Adams, I.R., Jackson, I.J., van 632x.12181. Heyningen, V. and Yeyati, P.L. Krejciova, Z., De Sousa, P., (2014) Kdm3a lysine demethylase Manson, J., Ironside, J.W. and Jägle, S., Rönsch, K., Timme, S., is an Hsp90 client required for Head, M.W. (2014) Human Tonsil- Andrlová, H., Bertrand, M., Jäger, cytoskeletal rearrangements Derived Follicular Dendritic-Like M., Proske, A., Schrempp, M., during spermatogenesis. Molecular Cells are Refractory to Human Yousaf, A., Michoel, T., Zeiser, Biology of the Cell E-pub 19 Prion Infection in Vitro and Traffic R., Werner, M., Lassmann, S. and February,DOI: 10.1091/mbc.E13- Disease-Associated Prion Protein Hecht, A. (2014) Silencing of the 08-0471. to Lysosomes. American Journal of EPHB3 tumor-suppressor gene in Pathology 184(1), 64-70,DOI: DOI: human colorectal cancer through Kiara, H., Jennings, A., Bronsvoort, 10.1016/j.ajpath.2013.09.013. decommissioning of a transcriptional B.M., Handel, I.G., Mwangi, S.T., enhancer. Proc Natl Acad Sci U S A Mbole-Kariuki, M., Conradie, Kuzemtseva, L., de la Torre, E., 111(13), 4886-4891,DOI: 10.1073/ V.A.N.W.I., Poole, E.J., Hanotte, Martín, G., Soldevila, F., Ait- pnas.1314523111. O., Coetzer, J.A., Woolhouse, Ali, T., Mateu, E. and Darwich, M.E. and Toye, P.G. (2014) A L. (2014) Regulation of toll-like Jeffrey, M., Martin, S., Chianini, longitudinal assessment of the receptors 3, 7 and 9 in porcine F., Eaton, S., Dagleish, M.P. and serological response to Theileria alveolar macrophages by different González, L. (2014) Incidence of parva and other tick-borne genotype 1 strains of porcine Infection in Prnp ARR/ARR Sheep parasites from birth to one year reproductive and respiratory following Experimental Inoculation in a cohort of indigenous calves syndrome virus. Veterinary with or Natural Exposure to in western Kenya. Parasitology Immunology and Immunopathology Classical Scrapie. PLoS One 9, FirstView, 1-10,DOI: 10.1017/ 158(3-4), 189-198,DOI: 10.1016/j. e91026,DOI: 10.1371/journal. s003118201400050x. vetimm.2014.01.009. pone.0091026. Kilpatrick, S., Gow, A.G., Foale, Lalor, S., Gunn-Moore, D.A., Jensen, K., Anderson, J.A. and R.D., Tappin, S.W., Carruthers, Cash, R., Foot, A., Reed, N. and Glass, E.J. (2014) Comparison H., Reed, N., Yool, D.A., Woods, Mellanby, R.J. (2014) Serum of small interfering RNA (siRNA) S., Marques, A.I., Jalan, R. and cardiac troponin iconcentrations in delivery into bovine monocyte- Mellanby, R.J. (2014) Plasma cats with anaemia - a preliminary, derived macrophages by cytokine concentrations in dogs single-centre observational study. transfection and electroporation. with a congenital portosystemic Journal of Small Animal Practice Veterinary Immunology and shunt. Veterinary Journal E-pub E-pub 20 March,DOI: 10.1111/ Immunopathology 158(3- 13 February,DOI: 10.1016/j. jsap.12210. 4), 224-232,DOI: 10.1016/j. tvjl.2014.01.007. vetimm.2014.02.002. Lalor, S., Schwartz, A.M., Titmarsh, Kilpatrick, S., Jacinto, A., Foale, H., Reed, N., Tasker, S., Boland, Jiang, Y.H., Xiao, C.T., Yin, R.D., Tappin, S.W., Burton, C., L., Berry, J., Gunn-Moore, D. and S.H., Gerber, P., Halbur, P. Frowde, P.E., Elwood, C.M., Mellanby, R.J. (2014) Cats with and Opriessnig, T. (2014) High Powell, R., Duncan, A., Mellanby, Inflammatory Bowel Disease and prevalence and genetic diversity of R.J. and Gow, A.G. (2014) Whole Intestinal Small Cell Lymphoma porcine bocaviruses (PBoV) in US blood manganese concentrations in Have Low Serum Concentrations pigs and identification of multiple dogs with primary hepatitis. Journal of 25-Hydroxyvitamin D. Journal novel PBoVs. Journal of General of Small Animal Practice E-pub 5 of Veterinary Internal Medicine Virology 95(2), 453-465,DOI: March,DOI: 10.1111/jsap.12196. E-pub 18 January,DOI: 10.1111/ 10.1099/vir.0.057042-0. jvim.12294. Kougioumtzis, A., Valergakis, Johansson, J.A. and Headon, D.J. G.E., Oikonomou, G., Arsenos, Lipschutz-Powell, D., Woolliams, (2014) Regionalisation of the skin. G. and Banos, G. (2014) Profile J.A. and Doeschl-Wilson, A.B. Seminars in Cell & Developmental and genetic parameters of dairy (2014) A unifying theory for genetic Biology 25-26, 3-10,DOI: 10.1016/j. cattle locomotion score and epidemiological analysis of binary semcdb.2013.12.007. lameness across lactation. Animal disease data. Genet Sel Evol 46(1), 8(1), 20-27,DOI: DOI: 10.1017/ 15,DOI: 10.1186/1297-9686-46-15.
35 Livesey, C.T., Payne, J.H., Sayers, A., Djønne, B. and Godfroid, chicken, an animal model of A.R., Whitaker, D.A., Burrough, E. J. (2014) Characterization of inherited retinal degeneration. and Macrae, A.I. (2014) Thyroxine Mycobacterium bovis from Humans Veterinary Ophthalmology 17(2), concentrations in ruminant health and Cattle in Namwala District, 113-119,DOI: 10.1111/vop.12051. monitoring and diagnostic blood Zambia. Veterinary Medicine samples. Veterinary Record 174(2), International (Article 187842),DOI: Meredith, A., Shaw, D., Brown, 47,DOI: 10.1136/vr.101498. 10.1155/2014/187842. J., Cleaveland, S. and Denwood, M.J. (2014) Coxiella burnetii (Q Lu, Z.H., Brown, A., Wilson, Malama, S., Muma, J., Munyeme, fever) seroprevalence in prey and A.D., Calvert, J.G., Balasch, M., M., Mbulo, G., Muwonge, A., Chola, predators in the United kingdom: Fuentes-Utrilla, P., Loecherbach, I., Djønne, B., Godfroid, J. and evaluation of infection in wild rodents, J., Turner, F., Talbot, R., Archibald, Johansen, T.B. (2014) Isolation foxes and domestic cats using a A.L. and Ait-Ali, T. (2014) Genomic and Molecular Characterization of modified ELISA. Transboundary and variation in macrophage-cultured Mycobacterium tuberculosis from Emerging Diseases Early View,DOI: European porcine reproductive Humans and Cattle in Namwala 10/1111/tbed.12211. and respiratory syndrome virus District, Zambia. Ecohealth Olot/91 revealed using ultra-deep 10(1007),DOI: 10.1007/s10393- Metheni, M., Echebli, N., next generation sequencing. Virol J 014-0940-0. Chaussepied, M., Ransy, C., 11(1), 42,DOI: 10.1186/1743-422x- Chereau, C., Jensen, K., Glass, 11-42. Matthews, P.C., Malik, A., Simmons, E., Batteux, F., Bouillaud, F. and R., Sharp, C., Simmonds, P. and Langsley, G. (2014) The level of H Lyons, S., Kapoor, A., Schneider, Klenerman, P. (2014) PARV4: An O -type oxidative stress regulates B.S., Wolfe, N.D., Culshaw, G., Emerging Tetraparvovirus. PLoS virulence of Theileria-transformed Corcoran, B., Durham, A.E., Pathogens 10(5), e1004036,DOI: leukocytes. Cell Microbiology 16(2), Burden, F., McGorum, B.C. and 10.1371/journal.ppat.1004036. 269-279,DOI: DOI: 10.1111/ Simmonds, P. (2014) Viraemic cmi.12218. frequencies and seroprevalence Mavrommatis, B., Offord, V., of non-primate hepacivirus and Patterson, R., Watson, M., Miele, A., Dhaliwal, K., Du Toit, equine pegiviruses in horses and Kanellos, T., Steinbach, F., N., Murchison, J.T., Dhaliwal, C., other mammalian species. Journal Grierson, S. and Werling, D. (2014) Brooks, H., Smith, S.H., Hirani, of General Virology E-pub 9 Global gene expression profiling N., Schwarz, T., Haslett, C., May,DOI: 10.1099/vir.0.065094-0. of myeloid immune cell subsets in Wallace, W.A. and McGorum, B. response to in vitro challenge with (2014) Chronic pleuropulmonary Mabbott, N., Kobayashi, A., porcine circovirus 2b. PLoS One fibrosis and elastosis of aged Sehgal, A., Bradford, B., Pattison, 9(3), e91081,DOI: 10.1371/journal. donkeys - similarities to human M. and Donaldson, D. (2014) Aging pone.0091081. pleuroparenchymal fibroelastosis and the mucosal immune system in (PPFE). Chest E-pub 6 March,DOI: the intestine. Biogerontology E-pub Mbole-Kariuki, M.N., Sonstegard, 10.1378/chest.13-1306. 5 April,DOI: 10.1007/s10522-014- T., Orth, A., Thumbi, S.M., 9498-z. Bronsvoort, B.M.d.C., Kiara, H., Moniuszko, A., Rückert, C., Alberdi, Toye, P., Conradie, I., Jennings, M.P., Barry, G., Stevenson, B., Mabbott, N.A. and Gray, D. (2014) A., Coetzer, K., Woolhouse, M.E.J., Fazakerley, J.K., Kohl, A. and Identification of Co-Expressed Hanotte, O. and Tapio, M. (2014) Bell-Sakyi, L. (2014) Coinfection of Gene Signatures in Mouse B1, Genome-wide analysis reveals tick cell lines has variable effects Marginal Zone and B2 B-cell the ancient and recent admixture on replication of intracellular Populations. Immunology 141(1), history of East African Shorthorn bacterial and viral pathogens. 79-95,DOI: 10.1111/imm.12171. Zebu from Western Kenya. Heredity Ticks and Tick-borne Diseases E-pub 16 April,DOI: 10.1038/ E-pub 29 March,DOI: 10.1016/j. Macfarlane, J.M., Lambe, N.R., hdy.2014.31. ttbdis.2014.01.010. Matika, O., Johnson, P.L., Wolf, B.T., Haresign, W., Bishop, S. and McCutcheon, S., Langeveld, J.P., Mooney, J.E., Summers, K.M., Bunger, L. (2014) Effect and mode Tan, B.C., Gill, A.C., de Wolf, C., Gongora, M., Grimmond, S.M., of action of the Texel muscling Martin, S., Gonzalez, L., Alibhai, J., Campbell, J.H., Hume, D.A. and QTL (TM-QTL) on carcass traits Blanco, A.R., Campbell, L., Hunter, Rolfe, B.E. (2014) Transcriptional in purebred Texel lambs. Animal N. and Houston, E.F. (2014) Prion switching in macrophages E-pub 6 May,DOI: 10.1017/ Protein-Specific Antibodies that associated with the peritoneal S1751731114001104. Detect Multiple TSE Agents with foreign body response. Immunology High Sensitivity. PLoS One 9(3), and Cell Biology E-pub 18 Mackenzie, N.C., Staines, K.A., e91143,DOI: 10.1371/journal. March,DOI: 10.1038/icb.2014.19. Zhu, D., Genever, P. and Macrae, pone.0091143. V.E. (2014) miRNA-221 and Morikawa, H., Ohkura, N., miRNA-222 synergistically function McKibbin, M., Ali, M., Inglehearn, Vandenbon, A., Itoh, M., Nagao- to promote vascular calcification. C., Shires, M., Boyle, K. and Sato, S., Kawaji, H., Lassmann, Cell Biochem Funct 32(2), 209- Hocking, P.M. (2014) Spectral T., Carninci, P., Hayashizaki, Y., 216,DOI: 10.1002/cbf.3005. domain optical coherence Forrest, A.R., Standley, D.M., Date, tomography imaging of the H., Sakaguchi, S. and FANTOM Malama, S., Johansen, T.B., posterior segment of the eye in the Consortium (2014) Differential Muma, J., Mbulo, J., Muwonge, retinal dysplasia and degeneration roles of epigenetic changes and
36 Foxp3 expression in regulatory Cao, Q., Yang, H. and Meng, X.J. One 9(1), e83144,DOI: 10.1371/ T cell-specific transcriptional (2014) Computer-aided codon-pairs journal.pone.0083144. regulation. Proc Natl Acad Sci deoptimization of the major envelope U S A 111(14), 5289-5294,DOI: GP5 gene attenuates porcine Paris, J.K., Wills, S., Balzer, H.J., 10.1073/pnas.1312717110. reproductive and respiratory syndrome Shaw, D.J. and Gunn-Moore, D.A. virus. Virology 450-451, 132-139,DOI: (2014) Enteropathogen co-infection Morota, G., Abdollahi-Arpanahi, 10.1016/j.virol.2013.12.009. in UK cats with diarrhoea. BMC Vet R., Kranis, A. and Gianola, D. Res 10(1), 13,DOI: 10.1186/1746- (2014) Genome-enabled prediction Nishiyama, S., Dutia, B.M., Stewart, 6148-10-13. of quantitative traits in chickens J., Meredith, A.L., Shaw, D.J., using genomic annotation. BMC Simmonds, P. and Sharp, C.P. Pérez O’Brien, A.M., Utsunomiya, Genomics 15,DOI: 10.1186/1471- (2014) The identification of novel Y.T., Mészáros, G., Bickhart, 2164-15-109. anelloviruses with broad diversity D.M., Liu, G.E., Van Tassell, in UK rodents. Journal of General C.P., Sonstegard, T.S., Da Silva, Murray, A., Dineen, A., Kelly, Virology E-pub 17 April,DOI: M.V., Garcia, J.F. and Sölkner, P., McGoey, K., Madigan, G., 10.1099/vir.0.065219-0. J. (2014) Assessing signatures Nighallchoir, E. and Gunn-Moore, of selection through variation in D. (2014) Nosocomial spread of Nuttall, T., Doug, R. and Roberts, linkage disequilibrium between Mycobacterium bovis in domestic E. (2014) Life-long diseases need taurine and indicine cattle. Genetics cats. Journal of Feline Medicine life-long treatment: long-term safety Selection Evolution 46(1), 19,DOI: and Surgery E-pub 7 April,DOI: of ciclosporin in canine atopic 10.1186/1297-9686-46-19. 10.1177/1098612x14529768. dermatitis. Veterinary Record 174(Suppt. 2), 3-12,DOI: 10.1136/ Philbey, A.W., Krause, S. and Murray, J.M.D., McMullin, P., vr.102471. Jefferies, R. (2014) Verminous Handel, I. and Hastie, P.M. (2014) pneumonia and enteritis due to Comparison of intestinal contents Oikonomou, G., Banos, G., hyperinfection with Aelurostrongylus from different regions of the equine Machado, V., Caixeta, L. and Bicalho, abstrusus in a kitten. Journal of gastrointestinal tract as inocula for R.C. (2014) Short communication: Comparative Pathology E-pub 11 use in an in vitro gas production Genetic characterization of digital February(Article),DOI: 10.1016/j. technique. Animal Feed Science cushion thickness. Journal of jcpa.2014.02.001. and Technology 187, 98-103,DOI: Dairy Science 97(1), 532-536,DOI: 10.1016/j.anifeedsci.2013.10.005. 10.3168/jds.2013-7212. Pieperhoff, S., Wilson, K.S., Baily, J., de Mora, K., Maqsood, S., Muwonge, A., Oloya, J., Kankya, C., Olivry, T., Saridomichelakis, Vass, S., Taylor, J., Del-Pozo, J., Nielsen, S., Godfroid, J., Skjerve, M., Nuttall, T., Bensignor, E., MacRae, C.A., Mullins, J.J. and E., Djonne, B. and Johansen, T.B. Griffin, C.E. and Hill, P.B. (2014) Denvir, M.A. (2014) Heart on a (2014) Molecular characterization of Validation of the Canine Atopic plate. histological and functional Mycobacterium avium subspecies Dermatitis Extent and Severity assessment of isolated adult hominissuis isolated from humans, Index (CADESI)-4, a simplified zebrafish hearts maintained in cattle and pigs in the Uganda cattle severity scale for assessing skin culture 9(5), e96771,DOI: 10.1371/ corridor using VNTR analysis. lesions of atopic dermatitis in dogs. journal.pone.0096771. Infection Genetics and Evolution Veterinary Dermatology E-pub 25 21, 184-191,DOI: 10.1016/j. January,DOI: 10.1111/vde.12107. Pirie, R.S. (2014) Recurrent airway meegid.2013.11.012. obstruction: A review. Equine Vet Opriessnig, T., Gerber, P.F., Xiao, J 46(3), 276-288,DOI: 10.1111/ Nadaf, J., Berri, C., Dunn, I., C.T., Mogler, M. and Halbur, P.G. evj.12204. Godet, E., Le Bihan-Duval, E. (2014) A commercial vaccine based and de Koning, D.J. (2014) An on PCV2a and an experimental Pirie, R.S., Jago, R.C. and Expression QTL of Closely Linked vaccine based on a variant mPCV2b Hudson, N.P. (2014) Equine Grass Candidate Genes Affects pH are both effective in protecting pigs Sickness. Equine Vet J E-pub 28 of Meat in Chickens. Genetics against challenge with a 2013 U.S. February,DOI: 10.1111/evj.12254. E-pub 3 January,DOI: 10.1534/ variant mPCV2b strain. Vaccine genetics.113.160440. 32(2), 230-237,DOI: 10.1016/j. Plinston, C., Hart, P., Hunter, N., vaccine.2013.11.010. Manson, J.C. and Barron, R.M. Nguyen, D.V., Pham, A.H., Nguyen, (2014) Increased susceptibility C.V., Ngo, H.T., Carrique-Mas, J., Ozdemir, D.D. and Hohenstein, of transgenic mice expressing Vo, H.B., Campbell, J., Baker, P. (2014) Wt1 in the kidney - A human PrP to experimental sheep S., Farrar, J., Woolhouse, M.E., tale in mouse models. Pediatric BSE is not due to increased agent Bryant, J.E. and Simmonds, Nephrology 29(4), 687-693,DOI: titre in sheep brain tissue. Journal P. (2014) Prevalence, genetic 10.1007/s00467-013-2673-7. of General Virology E-pub 14 diversity and recombination of May,DOI: 10.1099/vir.0.065730-0. species G enteroviruses infecting Pang, L.Y., Gatenby, E.L., Kamida, A., pigs in Vietnam. Journal of General Whitelaw, B.A., Hupp, T.R. and Argyle, Polak, M.E., Thirdborough, S.M., Virology 95(3), 549-556,DOI: D.J. (2014) Global Gene Expression Ung, C.Y., Elliott, T., Healy, E., 10.1099/vir.0.061978-0. Analysis of Canine Osteosarcoma Freeman, T.C. and Ardern-Jones, Stem Cells Reveals a Novel Role M.R. (2014) Distinct Molecular Ni, Y.Y., Zhao, Z., Opriessnig, T., for COX-2 in Tumour Initiation. PLoS Signature of Human Skin Subramaniam, S., Zhou, L., Cao, D.,
37 Langerhans Cells Denotes Critical Rebourcet, D., O’Shaughnessy, in the zebra finch (Taeniopygia Differences in Cutaneous Dendritic P.J., Pitetti, J.-L., Monteiro, A., guttata). General and Comparative Cell Immune Regulation. Journal O’Hara, L., Milne, L., Tsai, Y.T., Endocrinology 202, 26-34,DOI: of Investigative Dermatology Cruickshanks, L., Riethmacher, 10.1016/j.ygcen.2014.04.006. 134(3), 695-703,DOI: 10.1038/ D., Guillou, F., Mitchell, R.T., Van jid.2013.375. ’t Hof, R., Freeman, T.C., Nef, Sadeyen, J.R., Kaiser, P., Stevens, S. and Smith, L.B. (2014) Sertoli M.P. and Dziva, F. (2014) Analysis Prebble, J.L. and Meredith, A.L. cells control peritubular myoid cell of immune responses induced (2014) Food and water intake fate and support adult Leydig cell by avian pathogenic Escherichia and selective feeding in rabbits development in the prepubertal coli infection in turkeys and on four feeding regimes. J Anim testis. Development 141(10), 2139- their association with resistance Physiol Anim Nutr (Berl) E-pub 11 2149,DOI: 10.1242/dev.107029. to homologous re-challenge. January,DOI: 10.1111/jpn.12163. Veterinary Research 45(1), 19,DOI: Ressel, L., Else, R.W., Poli, A. 10.1186/1297-9716-45-19. Pridans, C., Lillico, S., Whitelaw, and Argyle, D.J. (2014) Aberrant B. and Hume, D.A. (2014) Subcellular Immunolocalization of Sanchez-Molano, E., Woolliams, Lentiviral vectors containing mouse NOTCH-1 Activated Intracellular J.A., Blott, S.C. and Wiener, P. Csf1r control elements direct Domain in Feline Mammary (2014) Assessing the impact of macrophage-restricted expression Tumours. Journal of Comparative genomic selection against hip in multiple species of birds and Pathology 150(4), 366-372,DOI: dysplasia in the Labrador Retriever mammals. Molecular Therapy — 10.1016/j.jcpa.2013.11.213. dog. Journal of Animal Breeding Methods & Clinical Development and Genetics 131(2), 134-145,DOI: 1(Article 14010),DOI: 10.1038/ Riaz, A., Dry, I., Levy, C., Hopkins, DOI: 10.1111/jbg.12056. mtm.2014.10. J., Grey, F., Shaw, D. and Dalziel, R. (2014) Ovine herpesvirus-2 Sargison, N.D. (2014) Sustainable Pumirat, P., Broek, C.V., encoded microRNAs target virus helminth control practices in Juntawieng, N., Muangsombut, V., genes involved in virus latency. the United Kingdom. Small Kiratisin, P., Pattanapanyasat, K., Journal of General Virology Ruminant Research 118(1- Stevens, J.M., Stevens, M.P. and 95(2), 472-480,DOI: 10.1099/ 3), 35-40,DOI: 10.1016/j. Korbsrisate, S. (2014) Analysis vir.0.059303-0. smallrumres.2013.12.019. of the Prevalence, Secretion and Function of a Cell Cycle-Inhibiting Riggio, V., Abdel-Aziz, M., Matika, Sauter, K., Pridans, C., Sehgal, Factor in the Melioidosis Pathogen O., Moreno, C.R., Carta, A. and A., Tsai, Y.T., Bradford, B., Raza, Burkholderia pseudomallei. PLoS Bishop, S.C. (2014) Accuracy of S., Moffat, L., Gow, D.J., Beard, One 9(5), e96298,DOI: 10.1371/ genomic prediction within and P., Mabbott, N., Smith, L. and journal.pone.0096298. across populations for nematode Hume, D. (2014) Pleiotropic effects resistance and body weight traits in of extended blockade of CSF1R Qi, J., Foroughi Asl, H., Bjorkegren, sheep. Animal 8(4), 520-528,DOI: signaling in adult mice. Journal J. and Michoel, T. (2014) kruX: 10.1017/s1751731114000081. of Leukocyte Biology E-pub 20 Matrix-based non-parametric eQTL March,DOI: 10.1189/jlb.2A0114- discovery. BMC Bioinformatics Riggio, V., Pong-Wong, R., Salle, 006R. 15(1), 11,DOI: 10.1186/1471-2105- G., Usai, M.G., Casu, S., Moreno, 15-11. C.R., Matika, O. and Bishop, S.C. Schmidl, C., Renner, K., Peter, K., (2014) A joint analysis to identify loci Eder, R., Lassmann, T., Balwierz, Rasoli, M., Yeap, S.K., Tan, S.W., underlying variation in nematode P.J., Itoh, M., Nagao-Sato, S., Moeini, H., Ideris, A., Bejo, M.H., resistance in three European sheep Kawaji, H., Carninci, P., Suzuki, Alitheen, N.B.M., Kaiser, P. and populations. Journal of Animal H., Hayashizaki, Y., Andreesen, Omar, A.R. (2014) Alteration in Breeding and Genetics E-pub 8 R., Hume, D.A., Hoffmann, P., lymphocyte responses, cytokine January,DOI: 10.1111/jbg.12071. Forrest, A.R.R., Kreutz, M.P., and chemokine profiles in chickens Edinger, M. and Rehli, M. (2014) infected with genotype VII and Rutherford, K.M.D., Piastowska- Transcription and enhancer profiling VIII velogenic Newcastle disease Ciesielska, A., Donald, R.D., in human monocyte subsets. Blood virus. Comparative Immunology, Robson, S.K., Ison, S.H., Jarvis, E-pub 26 March,DOI: 10.1182/ Microbiology and Infectious S., Brunton, P.J., Russell, J.A. and blood-2013-02-484188. Diseases 37(1), 11-21,DOI: Lawrence, A.B. (2014) Prenatal 10.1016/j.cimid.2013.10.003. stress produces anxiety prone Schmidt, V., Williams, N., female offspring and impaired Pinchbeck, G., Corless, C., Shaw, Raza, S., Barnett, M.W., Barnett- maternal behaviour in the domestic S., McEwan, N., Dawson, S. and Itzhaki, Z., Amit, I., Hume, D.A. pig. Physiology and Behavior Nuttall, T. (2014) Antimicrobial and Freeman, T.C. (2014) Analysis 129, 255-264,DOI: 10.1016/j. resistance and characterisation of of the transcriptional networks physbeh.2014.02.052. staphylococci isolated from healthy underpinning the activation Labrador retrievers in the United of murine macrophages by Ryan, C.P., Dawson, A., Sharp, Kingdom. BMC Veterinary Research inflammatory mediators. Journal P.J., Meddle, S.L. and Williams, 10, 17,DOI: 10.1186/1746-6148- of Leukocyte Biology E-pub 10 T.D. (2014) Circulating breeding 10-17. April,DOI: 10.1189/jlb.6HI0313- and pre-breeding prolactin and LH 169R. are not associated with clutch size Scorza, V., Willmott, A., Gunn-
38 Moore, D. and Lappin, M.R. (2014) burden, neuroinflammation and encephalopathy in dogs with Cryptosporidium felis in faeces neurodegeneration in HAART- congenital portosystemic shunts. from cats in the UK. Veterinary treated HIV positive injecting drug PLoS One 9(1), e82303,DOI: Record E-pub 29 April,DOI: users. J Neurovirology E-pub 14 10.1371/journal.pone.0082303. 10.1136/vr.102205. January,DOI: 10.1007/s13365- 013-0225-3. Tobin, V., Arechaga, G., Brunton, Servant-Delmas, A., Laperche, S., P., Russell, J., Leng, G., Ludwig, M. Lionnet, F., Sharp, C., Simmonds, Smith, S.H., Wilson, A.D., Van and Douglas, A. (2014) Oxytocinase P. and Lefrere, J.J. (2014) Human Ettinger, I., Macintyre, N., Archibald, in the female rat hypothalamus: parvovirus 4 infection in low- A.L. and Ait-ali, T. (2014) Down- a novel mechanism controlling and high-risk French individuals. regulation of mechanisms involved oxytocin neurons during lactation. Transfusion 54(3), 744-745,DOI: in cell transport and maintenance Journal of Neuroendocrinology 10.1111/trf.12512. of mucosal integrity in pigs infected 26(4), 205-216,DOI: 10.1111/ with Lawsonia intracellularis. jne.12141. Sevane, N., Armstrong, E., Wiener, Veterinary Research 45(1), 55,DOI: P., Pong Wong, R. and Dunner, S. 10.1186/1297-9716-45-55. Todd, D.L., Roughsedge, T. and (2014) Polymorphisms in twelve Woolliams, J.A. (2014) Genomic candidate genes are associated Stephen, L.A., Johnson, E.J., Davis, selection using beef commercial with growth, muscle lipid profile G.M., McTeir, L., Pinkham, J., carcass phenotypes. Animal and meat quality traits in eleven Jaberi, N. and Davey, M.G. (2014) 8(3), 388-394,DOI: 10.1017/ European cattle breeds. Molecular The chicken Left Right Organiser S1751731113002231. Biology Reports E-pub 10 April,DOI: has non-motile cilia which are lost 10.1007/s11033-014-3343-y. in a stage dependent manner in the Tsairidou, S., Woolliams, J.A., Allen, talpid ciliopathy. Genesis E-pub 2 A.R., Skuce, R.A., McBride, S.H., Sharma, R., Livesey, M.R., Wyllie, April,DOI: 10.1002/dvg.22775. Wright, D.M., Bermingham, M.L., D.J., Proudfoot, C., Whitelaw, Pong-Wong, R., Matika, O., McDowell, B., Hay, D.C. and Donadeu, X. Stratford, C.H., Lester, H.E., S.W.J., Glass, E.J. and Bishop, (2014) Generation of functional Morgan, E.R., Pickles, K.J., Relf, S.C. (2014) Genomic prediction neurons from feeder-free, V., McGorum, B.C. and Matthews, for tuberculosis resistance in dairy keratinocyte-derived equine J.B. (2014) A questionnaire study cattle. PLoS One 9(5), e96728,DOI: induced pluripotent stem cells. of equine gastrointestinal parasite 10.1371/journal.pone.0096728. Stem Cells and Development control in Scotland. Equine Vet J Turner, F.S. (2014) Assessment E-pub 18 February,DOI: 10.1089/ 46(1), 25-31,DOI: DOI: 10.1111/ of insert sizes and adapter scd.2013.0565. evj.12101. content in fastq data from NexteraXT libraries. Frontiers in Sheridan, V., Polychronopoulos, L., Stratford, C.H., Lester, H.E., Microbiology 5(JAN),DOI: 10.3389/ Dutia, B.M. and Ebrahimi, B. (2014) Pickles, K.J., McGorum, B.C. fmicb.2014.00005. A Shutoff and Exonuclease Mutant and Matthews, J.B. (2014) An of Murine Gammaherpesvirus-68 investigation of anthelmintic Utsunomiya, Y.T., Bomba, L., Yields Infectious Virus and efficacy against strongyles on Lucente, G., Colli, L., Negrini, R., Causes RNA loss in Type I equine yards in Scotland. Equine Lenstra, J.A., Erhardt, G., Garcia, Interferon Receptor Knock-Out Vet J 46(1), 17-24,DOI: DOI: J.F., Ajmone-Marsan, P., Moazami- cells. Journal of General Virology 10.1111/evj.12079. Goudarzi, K., Williams, J., Wiener, E-pub 19 February,DOI: 10.1099/ P., Olsaker, I., Kantanen, J., vir.0.059329-0. The FANTOM Consortium Dunner, S., Cañón, J., Rodellar, and the RIKEN PMI and CLST C., Martín-Burriel, I., Valentini, Sinclair, C., Gaunt, E., Simmonds, (DGT) (2014) A promoter-level A., Zanotti, M., Holm, L.E., P., Broomfield, D., Nwafor, N., mammalian expression atlas. Eythorsdottir, E., Mommens, G., Wellington, L., Templeton, K., Nature 507(7493), 462-470,DOI: Polygen, V.H., Nijman, I.J., Dolf, G. Willocks, L., Schofield, O. and 10.1038/nature13182. and Bradley, D.G. (2014) Revisiting Harvala, H. (2014) Atypical hand, AFLP fingerprinting for an unbiased foot, and mouth disease associated Thumbi, S., Bronsvoort, M., Poole, assessment of genetic structure with coxsackievirus A6 infection, J., Kiara, H., Toye, P., Ndila, M., and differentiation of taurine and Edinburgh, United Kingdom, Conradie van Wyk, I., Jennings, A., zebu cattle. BMC Genetics 15,DOI: January to February 2014. Euro Handel, I., Coetzer, J.A.W., Steyl, 10.1186/1471-2156-15-47. Surveillance 19(12), 6. J., Hanotte, O. and Woolhouse, M. (2014) Parasite co-infections Van Nguyen, D., Harvala, H., Ngole, Skaarud, A., Woolliams, and their impact on survival of E.M., Delaporte, E., Woolhouse, J.A. and Gjoen, H.M. (2014) indigenous cattle. PLoS One 9(2), M.E., Peeters, M. and Simmonds, Optimising resources and e76324,DOI: 10.1371/journal. P. (2014) High rates of infection management of genetic variation pone.0076324. with novel enterovirus variants in in fish-breeding schemes with wild populations of mandrills and multiple traits. Aquaculture Tivers, M.S., Handel, I., Gow, other old world monkey species. 420, 133-138,DOI: 10.1016/j. A.G., Lipscomb, V.J., Jalan, Journal of Virology 88(11), 5967- aquaculture.2013.10.033. R. and Mellanby, R.J. (2014) 5976,DOI: 10.1128/JVI.00088-14. Hyperammonemia and systemic Smith, D.B., Simmonds, P. and inflammatory response syndrome Vliegenthart, A.D., Starkey Lewis, P., Bell, J.E. (2014) Brain viral predicts presence of hepatic Tucker, C.S., Del Pozo, J., Rider, S.,
39 Antoine, D.J., Dubost, V., Westphal, Whiffin, N., Hosking, F.J., Farrington, Xue, J., Schmidt, S., Sander, J., M., Moulin, P., Bailey, M.A., Moggs, S.M., Palles, C., Dobbins, S.E., Draffehn, A., Krebs, W., Quester, J.G., Goldring, C.E., Park, B.K. Zgaga, L., Lloyd, A., Kinnersley, B., I., De Nardo, D., Gohel, T., Emde, and Dear, J.W. (2014) Retro- Gorman, M., Tenesa, A., Broderick, M., Schmidleithner, L., Ganesan, orbital Blood Acquisition Facilitates P., Wang, Y., Barclay, E., Hayward, H., Nino-Castro, A., Mallmann, M., Circulating microRNA Measurement C., Martin, L., Buchanan, D.D., Labzin, L., Theis, H., Kraut, M., in Zebrafish with Paracetamol Ko Win, A., Hopper, J., Jenkins, Beyer, M., Latz, E., Freeman, T.C., Hepatotoxicity. Zebrafish E-pub 13 M., Lindor, N.M., Newcomb, P.A., Ulas, T. and Schultze, J. (2014) March,DOI: 10.1089/zeb.2013.0912. Gallinger, S., Conti, D., Schumacher, Transcriptome-Based Network Vliegenthart, A.D., Tucker, C.S., F., Casey, G., Liu, T., Campbell, Analysis Reveals a Spectrum Model Del Pozo, J. and Dear, J.W. (2014) H., Lindblom, A., Houlston, R.S., of Human Macrophage Activation. Zebrafish as model organisms Tomlinson, I.P. and Dunlop, M.G. Immunity 40(2), 274-288,DOI: for studying drug induced liver (2014) Identification of susceptibility 10.1016/j.immuni.2014.01.006. injury. British Journal of Clinical loci for colorectal cancer in a Pharmacology E-pub 29 April,DOI: genome-wide meta-analysis. Human Yadav, M.C., Huesa, C., Narisawa, 10.1111/bcp.12408. Molecular Genetics E-pub 29 S., Hoylaerts, M.F., Moreau, A., May,DOI: 10.1093/hmg/ddu177. Farquharson, C. and Millan, J.L. Wagner, S., Gally, D.L. and Argyle, (2014) Ablation of Osteopontin S.A. (2014) Multidrug-resistant Wishart, T.M., Mutsaers, C.A., Improves the Skeletal Phenotype Escherichia coli from canine Riessland, M., Reimer, M.M., of Phospho1 Mice. Journal of Bone urinary tract infections tend to have Hunter, G., Hannam, M.L., Eaton, and Mineral Research E-pub 13 commensal phylotypes, lower S.L., Fuller, H.R., Roche, S.L., May,DOI: 10.1002/jbmr.2281. prevalence of virulence determinants Somers, E., Morse, R., Young, P.J., and ampC-replicons. Vet Microbiol Lamont, D.J., Hammerschmidt, M., Yamane, F., Nishikawa, Y., 169(3-4), 171-178,DOI: 10.1016/j. Joshi, A., Hohenstein, P., Morris, Matsui, K., Asakura, M., Iwasaki, vetmic.2014.01.003. G.E., Parson, S.H., Skehel, P.A., E., Watanabe, K., Tanimoto, H., Becker, T., Robinson, I.M., Becker, Sano, H., Fujiwara, Y., Stanley, Wami, W.M., Nausch, N., Bauer, K., C.G., Wirth, B. and Gillingwater, E.R., Kanayama, N., Mabbott, Midzi, N., Gwisai, R., Simmonds, T.H. (2014) Dysregulation of N.A., Magari, M. and Ohmori, H. P., Mduluza, T., Woolhouse, M. ubiquitin homeostasis and beta- (2014) CSF-1 receptor-mediated and Mutapi, F. (2014) Comparing catenin signaling promote spinal differentiation of a new type of parasitological vs serological muscular atrophy. Journal of monocytic cell with B cell-stimulating determination of Schistosoma Clinical Investigation 124(4), 1821- activity: its selective dependence on haematobium infection prevalence in 1834,DOI: 10.1172/jci71318. IL-34. Journal of Leukocyte Biology preschool and primary school-aged 95(1), 19-31,DOI: DOI: 10.1189/ children: implications for control Witteveldt, J., Blundell, R., jlb.0613311. programmes. Parasitology, 1-9,DOI: Maarleveld, J.J., McFadden, N., 10.1017/s0031182014000213. Evans, D.J. and Simmonds, P. Yao, Y., Smith, L.P., Nair, V. (2014) The influence of viral RNA and Watson, M. (2014) An avian Wedley, A.L., Dawson, S., Maddox, secondary structure on interactions retrovirus uses canonical expression T.W., Coyne, K.P., Pinchbeck, G.L., with innate host cell defences. and processing mechanisms to Clegg, P., Jamrozy, D., Fielder, Nucleic Acids Research 42(5), 3314- generate viral microRNA. Journal M.D., Donovan, D., Nuttall, T. and 3329,DOI: 10.1093/nar/gkt1291. of Virology 88(1), 2-9,DOI: 10.1128/ Williams, N.J. (2014) Carriage of JVI.02921-13. Staphylococcus species in the Wolc, A., Arango, J., Jankowski, veterinary visiting dog population T., Dunn, I., Settar, P., Fulton, J.E., Zatoń-Dobrowolska, M., Mucha, A., in mainland UK: Molecular O’Sullivan, N.P., Preisinger, R., Wierzbicki, H., Morrice, D., Moska, characterisation of resistance Fernando, R.L., Garrick, D.J. and M., Dobrowolski, M. and Przysiecki, P. and virulence. Vet Microbiol Dekkers, J.C.M. (2014) Genome- (2014) Microsatellite polymorphism 170(1-2), 81-88,DOI: 10.1016/j. wide association study for egg and its association with body weight vetmic.2014.01.015. production and quality in layer and selected morphometrics of farm chickens. Journal of Animal Breeding red fox (Vulpes vulpes L.). Journal of Whenham, N., Wilson, P.W., Bain, and Genetics 131(3), 173-182,DOI: applied genetics E-pub 13 May,DOI: M.M., Stevenson, L. and Dunn, I.C. 10.1111/jbg.12086. 10.1007/s13353-014-0217-x. (2014) Comparative biology and expression of TENP, an egg protein Wylie, C.E., Shaw, D.J., Fordyce, Zhu, D., Mackenzie, N.C., Millan, related to the bacterial permeability- F.M., Lilly, A. and McGorum, B.C. J.L., Farquharson, C. and Macrae, increasing family of proteins. Gene (2014) Equine grass sickness in V.E. (2014) Upregulation of 538(1), 99-108,DOI: 10.1016/j. Scotland: A case-control study of IGF2 expression during vascular gene.2013.12.065. signalment- and meteorology- related calcification. Vet Microbiol 52(2), 77- risk factors. Equine Vet J 46(1), 64- 85,DOI: 10.1530/jme-13-0136. 71,DOI: DOI: 10.1111/evj.12077.
If you have any articles you would like to submit for the next Roslin Reporter, please contact: Patricia Hart, Head of Communications and Science Administration (email:[email protected] or phone: 0131 651 9192) or require imagery to accompany articles, please contact: Norrie Russell, Photographer (email:[email protected] or phone: 0131 651 9197)
40