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DATASHEET USA: [email protected] FOR IN VITRO RESEARCH USE ONLY Europe: [email protected] NOT FOR USE IN HUMANS OR ANIMALS China: [email protected] Choline kinase alpha Polyclonal Anbody Catalog number: 13520-1-AP Background Size: 39 μg/150 μl CHKA(Choline kinase alpha) is also named as CHK, CKI, EK(Ethanolamine kinase) Source: Rabbit and belongs to the choline/ethanolamine kinase family. Choline kinase (ATP:choline Isotype: IgG phosphotransferase) is a cytosolic enzyme that catalyzes the commied step in the Synonyms: synthesis of PC by the CDP-choline pathway. The enzyme catalyzes the CHKA; CHETK alpha, CHK, phosphorylaon of choline with ATP to form phosphocholine and CHKA, Choline kinase alpha, ADP(PMID:9506987). It has 2 isoforms produced by alternave splicing with the CK, CKI, EK, Ethanolamine molecular weight of 52 kDa and 50 kDa. kinase; Applications Tested applicaons: ELISA, WB, IHC, IP Cited applicaons: IHC, WB Species specificity: Human,Mouse,Rat; other species not tested. Cited species: Human, mouse, rat Caculated Choline kinase 439aa,52 kDa alpha MW: Immunohistochemical of paraffin- Observed Choline kinase 50 kDa,52 kDa embedded human liver using 13520- 1-AP(CHKA anbody) at diluon of alpha MW: 1:50 (under 10x lens) Posive WB detected in COLO 320 cells, mouse colon ssue Posive IP detected in Mouse spleen ssue Posive IHC detected in Human liver ssue Recommended diluon: WB: 1:200-1:2000 IP: 1:200-1:2000 IHC: 1:20-1:200 Applicaon key: WB = Western blong, IHC = Immunohistochemistry, IF = Immunofluorescence, IP = Immunoprecipitaon Immunohistochemical of paraffin- Immunogen information embedded human liver using 13520- 1-AP(CHKA anbody) at diluon of Immunogen: Ag4761 1:50 (under 40x lens) GenBank accession number: BC036471 Gene ID (NCBI): 1119 Full name: Choline kinase alpha Product information Purificaon method: Angen affinity purificaon Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. COLO 320 cells were subjected to SDS PAGE followed by western blot with 13520-1-AP(CHKA anbody) at diluon of 1:300 IP Result of an-CHKA (IP:13520-1-AP, 4ug; (IP:13520-1-AP, 4ug; Detecon:13520-1-AP 1:500) with mouse spleen ssue lysate 4000ug..

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Identification and Validation of Novel and More Effective Choline Kinase
www.nature.com/scientificreports OPEN Identifcation and validation of novel and more efective choline kinase inhibitors against Streptococcus pneumoniae Tahl Zimmerman1*, Valerie Chasten1, Juan Carlos Lacal2 & Salam A. Ibrahim1 Streptococcus pneumoniae choline kinase (sChoK) has previously been proposed as a drug target, yet the efectiveness of the frst and only known inhibitor of sChoK, HC-3, is in the millimolar range. The aim of this study was thus to further validate sChoK as a potential therapeutic target by discovering more powerful sChoK inhibitors. LDH/PK and colorimetric enzymatic assays revealed two promising sChoK inhibitor leads RSM-932A and MN58b that were discovered with IC50 of 0.5 and 150 μM, respectively, and were shown to be 2–4 magnitudes more potent than the previously discovered inhibitor HC-3. Culture assays showed that the minimum inhibitory concentration (MIC) of RSM- 932A and MN58b for S. pneumoniae was 0.4 μM and 10 μM, respectively, and the minimum lethal concentration (MLC) was 1.6 μM and 20 μM, respectively. Western blot monitoring of teichoic acid production revealed diferential patterns in response to each inhibitor. In addition, both inhibitors possessed a bacteriostatic mechanism of action, and neither interfered with the autolytic efects of vancomycin. Cells treated with MN58b but not RSM-932A were more sensitive to a phosphate induced autolysis with respect to the untreated cells. SEM studies revealed that MN58b distorted the cell wall, a result consistent with the apparent teichoic acid changes. Two novel and more highly potent putative inhibitors of sChoK, MN58b and RSM-932A, were characterized in this study.
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Table S1. List of Oligonucleotide Primers Used
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