DOCSLIB.ORG

TAKSONOMSKA ANALIZA KAPLJA (Cottus Sp.) V SLOVENIJI Z UPORABO MOLEKULARNIH METOD

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
TAKSONOMSKA ANALIZA KAPLJA (Cottus Sp.) V SLOVENIJI Z UPORABO MOLEKULARNIH METOD UNIVERZA V LJUBLJANI BIOTEHNIŠKA FAKULTETA ODDELEK ZA BIOLOGIJO Jernej BRAVNIČAR TAKSONOMSKA ANALIZA KAPLJA (Cottus sp.) V SLOVENIJI Z UPORABO MOLEKULARNIH METOD DIPLOMSKO DELO Univerzitetni študij TAXONOMIC ANALYSIS OF BULLHEAD (Cottus sp.) IN SLOVENIA WITH APPLICATION OF MOLECULAR METHODS GRADUATION THESIS University studies Ljubljana, 2012 Bravničar J. Taksonomska analiza kaplja (Cottus sp.) v Sloveniji z uporabo molekularnih metod. Diplomsko delo. Ljubljana, Univerza v Ljubljani, Biotehniška fakulteta, Oddelek za biologijo, 2012 Diplomsko delo je zaključek Univerzitetnega študija biologije. Opravljeno je bilo na Biotehniški fakulteti Univerze v Ljubljani, Oddelku za zootehniko, Katedri za genetiko, animalno biotehnologijo in imunologijo. Terensko delo se je odvijalo na območju Slovenije, Italije, Hrvaške, Bosne in Hercegovine in Srbije. Študijska komisija Oddelka za biologijo je za mentorja diplomskega dela imenovala višjega znanstvenega sodelavca dr. Aleša Snoja. Komisija za oceno dela in zagovor: Predsednik: doc. dr. Cene FIŠER Univerza v Ljubljani, Biotehniška fakulteta, Oddelek za biologijo Član: prof. dr. Peter TRONTELJ Univerza v Ljubljani, Biotehniška fakulteta, Oddelek za biologijo Član: viš. znan. sod. dr. Aleš SNOJ Univerza v Ljubljani, Biotehniška fakulteta, Oddelek za zootehniko Datum zagovora: 13.9.2012 Delo je rezultat lastnega raziskovalnega dela. Podpisani se strinjam z objavo svoje naloge v polnem tekstu na strani Digitalne knjiţnice Biotehniške fakultete. Izjavljam, da je naloga, ki sem jo oddal v elektronski obliki, identična tiskani verziji. Jernej Bravničar II Bravničar J. Taksonomska analiza kaplja (Cottus sp.) v Sloveniji z uporabo molekularnih metod. Diplomsko delo. Ljubljana, Univerza v Ljubljani, Biotehniška fakulteta, Oddelek za biologijo, 2012 KLJUČNA DOKUMENTACIJSKA INFORMACIJA (KDI) ŠD Dn DK UDK 59:592(043.2)=163.6 KG kapelj / Cottus / Balkan / filogeografija / mtDNA AV BRAVNIČAR, Jernej SA SNOJ, Aleš (mentor) KZ SI-1001 Ljubljana, Večna pot 111 ZA Univerza v Ljubljani, Biotehniška fakulteta, Oddelek za biologijo LI 2012 IN TAKSONOMSKA ANALIZA KAPLJA (Cottus sp.) V SLOVENIJI Z UPORABO MOLEKULARNIH METOD TD Diplomsko delo (Univerzitetni študij) OP VI, 51 str., 4 pregl., 11 sl., 61 vir. IJ sl JI sl/en AI Slovenijo naj bi na osnovi morfoloških podatkov naseljevali dve vrsti evropskega kaplja, Cottus gobio in C. metae. Za slednjega so znane tri lokalitete, predvideva pa se, da poseljuje areal od izvirnih delov Save do pritoka Kolpe. Tako Kolpo kot reke jadranskega povodja in dravskega porečja pa naj bi poseljeval C. gobio. V raziskavi smo ţeleli ugotoviti, ali se kaplji, ki naseljujejo areal vrste C. metae, na osnovi nukleotidnega zaporedja kontrolne regije mitohondrijske DNA (CR mtDNA) ločijo od vrste C. gobio. Poleg tega smo ţeleli ugotoviti, koliko filogeografskih linij kaplja naseljuje Slovenijo. Vzorčili smo tudi na širšem območju severozahodnega Balkana, ki na splošno velja za vročo točko biodiverzitete. Izkazalo se je, da kaplji C. metae na podlagi CR mtDNA tvorijo lastno filogeografsko linijo. Kaplji C. gobio, ki naseljujejo vodotoke jadranskega povodja in dravskega porečja, skupaj z ostalimi evropskimi pripadniki vrste tvorijo lasten klad. Z veliko verjetnostjo lahko trdimo, da kaplji, ki poseljujejo Kolpo, na molekularni osnovi ne spadajo v vrsto C. gobio. Ti namreč skupaj s haplotipi, ki jih najdemo v reki Uni, tvorijo lastno linijo. Filogenetske raziskave so pokazale, da se evropski kapelj v osnovi deli na dve sestrski skupini, ki po naši razlagi predstavljata dva časovno ločena kolonizacijska vala. Domnevamo, da je kaplju zahodni Balkan sluţil kot odskočna deska kolonizacije Evrope obeh valov. III Bravničar J. Taksonomska analiza kaplja (Cottus sp.) v Sloveniji z uporabo molekularnih metod. Diplomsko delo. Ljubljana, Univerza v Ljubljani, Biotehniška fakulteta, Oddelek za biologijo, 2012 KEY WORDS DOCUMENTATION DN Dn DC UDC 59:592(042.2)=163.6 CX bullhead / Cottus / Balkans / phylogeography / mtDNA AU BRAVNIČAR, Jernej AA SNOJ, Aleš (supervisor) PP SI-1001 Ljubljana, Večna pot 111 PB University of Ljubljana, Biotechnical Faculty, Department of Biology PY 2012 TI TAXONOMIC ANALYSIS OF BULLHEAD (Cottus sp.) IN SLOVENIA WITH APPLICATION OF MOLECULAR METHODS DT Graduation Thesis (University studies) NO VI, 51 p., 4 tab., 11 fig., 61 ref. LA sl AL sl/en AB Two morphologically defined European bullhead species, Cottus gobio and C. metae, are known to inhabit the rivers in Slovenia. There are three described localities of C. metae with presumed species range from the headwaters of the Sava river to the Kolpa confluence. C. gobio assumed range are the Kolpa and Drava watershed along with the rivers of the Adriatic drainage. The aim of this study was to assess whether the bullheads populating the C. metae range are genetically distinct from the neighboring C. gobio. We also tended to perform phylogeographic analysis to find out how many lineages of bullhead are there in Slovenia. Besides Slovenia, sampling was performed also in a wider area of northwestern Balkans, which is known as one of the main European biodiversity hot spots. Based upon CR mtDNA analysis, C. metae turned to be a distinctive phylogeographical lineage, while the sample-set of the Drava and Adriatic drainage was recognized as C. gobio. Bullheads in the Kolpa river poses haplotypes, which are mostly related to the ones found in the Una, and together form a distinctive phylogeographic lineage that was found to be related neither to C. gobio nor C. metae, and possibly represent a new species. Phylogenetic studies showed that European bullheads form two major groups, each of which were involved in two subsequent colonization waves of Europe. We suggest that both colonizations started in the western Balkans. IV Bravničar J. Taksonomska analiza kaplja (Cottus sp.) v Sloveniji z uporabo molekularnih metod. Diplomsko delo. Ljubljana, Univerza v Ljubljani, Biotehniška fakulteta, Oddelek za biologijo, 2012 KAZALO VSEBINE Ključna dokumentacijska informacija (KDI) III Key words documentation IV Kazalo vsebine V Kazalo preglednic VI Kazalo slik VI 1. UVOD 1 1.1. OPREDELITEV PROBLEMA 1 1.2. RAZNOLIKOST RODU Cottus V EVROPI 4 1.3. KOLONIZACIJSKA ZGODOVINA KAPLJA V EVROPI 8 2. MATERIALI IN METODE 12 2.1. VZORCI 12 2.2. METODE 18 2.2.1. PRIPRAVA VZORCEV IN IZOLACIJA DNK 18 2.2.2. POMNOŽEVANJE V VERIŽNI REAKCIJI S POLIMERAZO (PCR) 19 2.2.3. DOLOČANJE NUKLEOTIDNEGA ZAPOREDJA . 20 2.2.4. NASIČENOST SEKVENC 21 2.2.5. FILOGENETSKE IN FILOGEOGRAFSKE ANALIZE 21 3. REZULTATI 25 3.1. ZNAČILNOSTI ZAPOREDIJ 25 3.2. FILOGENETSKA DREVESA 28 3.3. MREŢNA ANALIZA HAPLOTIPOV 33 4. RAZPRAVA 36 4.1 KAPLJI V SLOVENIJI IN BLIŢNJI OKOLICI 36 4.2 POMEN BALKANSKEGA POLOTOKA PRI KOLONIZACIJI EVROPE 38 5. SKLEPI 43 6. POVZETEK 44 7. LITERATURA 46 V Bravničar J. Taksonomska analiza kaplja (Cottus sp.) v Sloveniji z uporabo molekularnih metod. Diplomsko delo. Ljubljana, Univerza v Ljubljani, Biotehniška fakulteta, Oddelek za biologijo, 2012 KAZALO PREGLEDNIC Preglednica 1: Delitev evropskega kaplja na vrste po Kottelat in Freyhof 2007 5 Preglednica 2: Seznam lokalitet s spremljajočimi podatki 13 Preglednica 3: Seznam haplotipov, ki smo jih uporabili v analizah 15 Preglednica 4: Seznam izoliranih vzorcev 26 KAZALO SLIK Slika 1: Navadni kapelj (Cottus gobio) iz Donave 1 Slika 2: Ostanki okostenelih luskbodci, pod prsno plavutjo 2 Slika 3: Zemljevid evropskih rek z areali kladov in vrst 6 Slika 4: Zemljevid lokalitet v Sloveniji 14 Slika 5 : Zemljevid lokalitet iz katerih haplotipe smo uporabili v analizah 17 Slika 6: Graf nasičenosti sekvenc s substitucijami 27 Slika 7: Filogenetsko drevo po metodi ML v programu PhyML 28 Slika 8: Filogenetsko drevo izdelano po Bayesovi metodi v programu BEAST 31 Slika 9: Filogram izdelan po Bayesovi metodi v programu BEAST 32 Slika 10: Mreţa haplotipov Balkana izrisana s programom Network 33 Slika 11: Mreţa evropskih haplotipov izrisana s programom Network 35 VI Bravničar J. Taksonomska analiza kaplja (Cottus sp.) v Sloveniji z uporabo molekularnih metod. Diplomsko delo. Ljubljana, Univerza v Ljubljani, Biotehniška fakulteta, Oddelek za biologijo, 2012 1. UVOD 1.1. OPREDELITEV PROBLEMA Slovenijo poseljujeta dve vrsti kaplja, Cottus gobio Linnaeus, 1758 in nedavno opisana C. metae Freyhof, Kottelat & Nolte, 2005 (Freyhof in sod. 2005, Kottelat in Freyhof 2007). Slika 1: Navadni kapelj (Cottus gobio) iz Donave C. metae (barjanski kapelj) naj bi naseljeval zgornje porečje Save, in sicer od njenih izvirov do sotočja s Kolpo. Za to vrsto so značilni okosteneli ostanki lusk pod prsno plavutjo (angl. prickles), kar v slovenščino prevajamo kot bodci. Bodci lahko pri osebkih, velikih okoli 8 cm ali več, izginejo (Freyhof in sod. 2005). Holotipski primerek izvira iz reke Iščice ob Igu pri Ljubljani, tej lokaciji pa pripada še deset opisanih paratipskih primerkov. Dodatnih primerkov, ki so prispevali k opisu karakteristik vrste, je še 12 iz neimenovanega potoka v Ljubljani in šest iz Save Bohinjke pri Bohinjski Bistrici (Freyhof in sod. 2005). Na podlagi teh lokacij je bil predlagan tudi zgoraj omenjeni areal vrste. 1 Bravničar J. Taksonomska analiza kaplja (Cottus sp.) v Sloveniji z uporabo molekularnih metod. Diplomsko delo. Ljubljana, Univerza v Ljubljani, Biotehniška fakulteta, Oddelek za biologijo, 2012 Vrsto C. gobio (navadni kapelj) v Sloveniji najdemo v rekah jadranskega povodja ter v porečjih Drave in Kolpe. Navadni kapelj je precej razširjena vrsta, ki na severu naseljuje vodotoke Švedske, potoke ob obalah Finske in Rusije ob Baltiku,
Load more

Recommended publications
  • EUROPEAN RED LIST of AMPHIBIANS Appenine Yellow-Bellied Toad (Bombina Pachypus)
    EUROPEAN RED LIST OF AMPHIBIANS Appenine Yellow-bellied Toad (Bombina pachypus) November 2011 Photo©Roberto Sindaco The Appenine Yellow-bellied Toad (Bombina Threats to this species are presumed to largely pachypus) is endemic to Italy, where it occurs south include loss and fragmentation of wetland habitat to of the Po Valley, through the Appenine region, drainage for intensive agricultural purposes. south to the southern tip of the Italian mainland. This species is protected under international laws. It It was formerly common, however, the species is listed on Appendix II of the Bern Convention and has declined in almost all of its range (with the Annex II and IV of the EU Habitats Directive. exception of Calabria, where populations remain stable) over the last ten years. It is listed as Endangered according to the IUCN Red List Categories and Criteria on the basis This species occurs in both terrestrial and of rapid recent population declines, suspected freshwater habitats and is commonly found to have been caused by the fungal disease in unshaded pools in forests and open areas, chytridiomycosis. including pools formed in ditches, irrigation areas, farmland, or pasture land. The IUCN Red List of Threatened Species™ - Regional Assessment EUROPEAN RED LIST OF AMPHIBIANS Common Toad (Bufo bufo) November 2011 Photograph © John Wilkinson The Common Toad (Bufo bufo) is a widespread It is listed on Appendix III of the Bern Convention species in Europe. It is generally common and, and is protected by national and sub-national adaptable and has been recorded from coniferous, legislation in many countries. It is recorded on many mixed and deciduous forests, groves, bushlands, national and sub-national Red Data books and lists.
    [Show full text]
  • Identification of Priority Areas for the Conservation of Stream Fish Assemblages: Implications for River Management in France A
    Identification of Priority Areas for the Conservation of Stream Fish Assemblages: Implications for River Management in France A. Maire, P. Laffaille, J.F. Maire, L. Buisson To cite this version: A. Maire, P. Laffaille, J.F. Maire, L. Buisson. Identification of Priority Areas for the Conservation of Stream Fish Assemblages: Implications for River Management in France. River Research and Applications, Wiley, 2016, 33 (4), pp.524-537. 10.1002/rra.3107. hal-01426354 HAL Id: hal-01426354 https://hal.archives-ouvertes.fr/hal-01426354 Submitted on 2 Jul 2021 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution| 4.0 International License IDENTIFICATION OF PRIORITY AREAS FOR THE CONSERVATION OF STREAM FISH ASSEMBLAGES: IMPLICATIONS FOR RIVER MANAGEMENT IN FRANCE A. MAIREa*,†, P. LAFFAILLEb,c, J.-F. MAIREd AND L. BUISSONb,e a Irstea; UR HYAX, Pôle Onema-Irstea Hydroécologie des plans d’eau; Centre d’Aix-en-Provence, Aix-en-Provence, France b CNRS; UMR 5245 EcoLab, (Laboratoire Ecologie Fonctionnelle et Environnement), Toulouse, France c Université de Toulouse, INP, UPS; EcoLab; ENSAT, Castanet Tolosan, France d ONERA, The French Aerospace Lab Composites Department, Châtillon, France e Université de Toulouse, INP, UPS; EcoLab, Toulouse, France ABSTRACT Financial and human resources allocated to biodiversity conservation are often limited, making it impossible to protect all natural places, and priority areas for protection must be identified.
    [Show full text]
  • Eel Sampling Protocols
    EEL SAMPLING PROTOCOLS I. Domingos Table of Contents 1. PROTOCOL FOR YELLOW AND SILVER EEL SAMPLING IN RIVERS………………………………………………………..7 1.1. Timing of surveys ........................................................................................................................... 7 1.2. Site selection .................................................................................................................................. 7 1.3. Length of the sampling reach ........................................................................................................ 9 1.4. Sampling procedures ..................................................................................................................... 9 1.5. Environmental data to collect in the field .................................................................................... 10 1.6. Biological data to collect in the field at each fish pass ................................................................. 10 1.7. Samples for laboratory analysis ................................................................................................... 11 1.8. Field equipment ........................................................................................................................... 12 1.9. R script to identify the silver eel stage from Durif et al. (2009) ................................................... 13 2. PROTOCOL TO ESTIMATE GLASS EEL RECRUITMENT………………………………………………………………………..15 2.1. Timing of surveys ........................................................................................................................
    [Show full text]
  • Assessment of the Conservation Status of Endemic Sculpin Cottus Haemusi (Cottidae) in the River Vit (Danube Tributary), Northwest Bulgaria E
    Knowledge and Management of Aquatic Ecosystems (2011) 403, 10 © ONEMA, 2011 http://www.kmae-journal.org DOI: 10.1051/kmae/2011071 Assessment of the conservation status of endemic sculpin Cottus haemusi (Cottidae) in the river Vit (Danube Tributary), northwest Bulgaria E. Petrova Uzunova(1) Received March 29, 2011 Revised July 14, 2011 Accepted July 25, 2011 ABSTRACT Key-words: Cottus haemusi (Marinov and Dikov, 1986. Acta Zool. Bulg. 3, 18–23) is an Cottus haemusi, endemic fish species that is restricted to the upper tributaries of the river distribution, Vit, Northwest Bulgaria. After its discovery in 1986, no further investigation abundance, of the C. haemusi population has been conducted. The aims of the present size, study were to determine its current population status based on the distri- conservation bution, abundance and size structure of the C. haemusi population and to measures analyse the main environmental parameters of its habitat. Five upland tri- butaries and the main river were examined in low-water periods in 2009 and 2010. Two-pass electrofishing surveys were performed at 14 sites to estimate species presence, abundance and size distribution. C. haemusi was only detected in two tributaries of the river Vit: Kostina and Toplja. The current investigation failed to find the species at previously recorded sites. The total distribution area of this species is estimated to be 16 200 m2. The observed abundance of the Vit sculpin ranged from 5.6 to 8.4 indivi- duals·100 m–2, with a mean of 7.0 individuals·100 m–2. Investigation of the size structure revealed the relatively low contribution of one-summer-old individuals.
    [Show full text]
  • Subsidiary Legislation 549.44 Flora, Fauna and Natural Habitats
    FLORA, FAUNA AND NATURAL HABITATS PROTECTION [S.L.549.44 1 SUBSIDIARY LEGISLATION 549.44 FLORA, FAUNA AND NATURAL HABITATS PROTECTION REGULATIONS 7th December, 2006 LEGAL NOTICE 311 of 2006, as amended by Legal Notices 426 of 2007, 162 of 2009, 94 of 2010, 322 of 2013 and 379 of 2016. 1. (1) The title of these regulations is the Flora, Fauna and Citation and entry Natural Habitats Protection Regulations. into force. (2) Part VIII of these regulations shall come into force on such a date as the Minister responsible for the environment may by notice in the Gazette appoint. (3) A notice under subregulation (2) may make such transitional provisions as appear to the Minister to be necessary or expedient in connection with the provisions thereby brought into force. 2. (1) The aim of these regulations is to contribute towards Scope. ensuring biodiversity in the territory of the Member States of the European Community through the conservation of natural habitats and of wild fauna and flora in the Maltese Islands. (2) Measures taken pursuant to these regulations shall be designed to maintain or restore, at favourable conservation status, natural habitats and species of wild fauna and flora of Community interest, and shall take account of economic, social and cultural requirements and regional and local characteristics. (3) These regulations provide the provisions required for the implementation in Malta of: (a) Council Directive 92/43/EEC of 21 May 1992 on the Conservation of Natural Habitats and of Wild Fauna and Flora, (b) Council Directive 79/409/EEC of 2 April 1979 on the Conservation of Wild Birds, (c) the Convention on Biological Diversity, (d) the Convention on the Conservation of European Wildlife and Natural Habitats, (e) the Convention on the Conservation of Migratory Species of wild Animals, and (f) the Protocol for Specially Protected Areas and Biological Diversity in the Mediterranean of the Barcelona Convention; they shall be read and construed as one with such legal instruments.
    [Show full text]
  • LE CHABOT DU LEZ (Cottus Petiti)
    SITE D’IMPORTANCE COMMUNAUTAIRE « LE LEZ » - FR 9101392 LE CHABOT DU LEZ (Cottus petiti) MONOGRAPHIE ET PERSPECTIVE POUR L’AMELIORATION DES CONNAISSANCES DE L’ESPECE Juin 2015 1 Sommaire Contexte ...................................................................................................................................................... 3 Introduction ................................................................................................................................................ 5 PREMIERE PARTIE ....................................................................................................................................... 6 Monographie du Chabot du Lez (Cottus Petiti) ............................................................................................ 6 1. Identité et statut de Cottus petiti ........................................................................................................ 7 1.1. Fiche d’identité et Classification .......................................................................................................... 7 1.2. Hypothèse sur l’origine génétique et taxonomique de Cottus petiti ..................................................... 7 1.3. Statut de l’espèce ................................................................................................................................ 8 2. L’environnement de Cottus petiti ........................................................................................................ 9 2.1. Aire de répartition ..............................................................................................................................
    [Show full text]
  • Revalidation of the Spanish Stone Loach Barbatula Hispanica (Lelek, 1987) (Teleostei, Nemacheilidae) According to Morphological and Mitochondrial Data
    Ann. Limnol. - Int. J. Lim. 2021, 57, 10 Available online at: © G.P.J. Denys et al., published by EDP Sciences, 2021 www.limnology-journal.org https://doi.org/10.1051/limn/2021007 RESEARCH ARTICLE Revalidation of the Spanish stone loach Barbatula hispanica (Lelek, 1987) (Teleostei, Nemacheilidae) according to morphological and mitochondrial data Gaël P.J. Denys1,2,*, Silvia Perea3, Agnès Dettai4, Camille Gauliard2,5, Henri Persat6 and Ignacio Doadrio3 1 Unité Mixte de Service Patrimoine Naturel – Centre d’expertise et de données (UMS 2006 OFB – CNRS – MNHN), Muséum national d’Histoire naturelle, 36 rue Geoffroy-Saint-Hilaire CP 41, 75005 Paris, France 2 UMR Biologie des organismes et écosystèmes aquatiques (BOREA 8067), MNHN, CNRS, IRD, SU, UCN, UA, 57 rue Cuvier CP26, 75005 Paris, France 3 National Museum of Natural Sciences MNCN, Spanish Research Council, Department of Biodiversity and Evolutionary Biology, José Gutiérrez Abascal 2 Str., 28006 Madrid, Spain 4 Institut de Systématique, Évolution, Biodiversité, ISYEB – UMR 7205–CNRS, MNHN, UPMC, EPHE, Muséum national d’Histoire naturelle, Sorbonne Universités, 57 rue Cuvier CP26, 75005 Paris, France 5 Maladie Infectieuse et Vecteurs, Écologie, Génétique, Évolution et Contrôle (MIVEGEC), IRD, CNRS UMR 5175, 911 avenue Agropolis, 34394 Montpellier cedex 5, France 6 Société Française d’Ichtyologie, Muséum national d’Histoire naturelle, 57 rue Cuvier CP26, 75005 Paris, France Received: 18 January 2021; Accepted: 15 April 2021 Abstract – This study revalidates Barbatula hispanica, previously considered a junior synonym of B. quignardi. This species is found in the Ebro drainage and in Cantabria (Spain) as well as in the Adour drainage (Southwestern France).
    [Show full text]
  • B 4358 LN 311 of 2006 ENVIRONMENT PROTECTION
    B 4358 L.N. 311 of 2006 ENVIRONMENT PROTECTION ACT (CAP. 435) DEVELOPMENT PLANNING ACT (CAP. 356) Flora, Fauna and Natural Habitats Protection Regulations, 2006 BY virtue of the powers conferred by articles 6, 9, 10(2), 11 and 23 of the Environment Protection Act and article 60 of the Development Planning Act, 1992, the Minister for Rural Affairs and the Environment has made the following regulations> - Citation and entry 1. (1) The title of these regulations is the Flora, Fauna and into force. Natural Habitats Protection Regulations, 2006. (2) Part VIII of these regulations shall come into force on such a date as the Minister responsible for the environment may by notice in the Gazette appoint. (3) A notice under paragraph (2) of this regulation may make such transitional provisions as appear to the Minister to be necessary or expedient in connection with the provisions thereby brought into force. Scope. 2. (1) The aim of these regulations is to contribute towards ensuring biodiversity in the territory of the Member States of the European Community through the conservation of natural habitats and of wild fauna and flora in the Maltese Islands. (2) Measures taken pursuant to these regulations shall be designed to maintain or restore, at favourable conservation status, natural habitats and species of wild fauna and flora of Community interest, and shall take account of economic, social and cultural requirements and regional and local characteristics. (3) These regulations provide the provisions required for the implementation in Malta
    [Show full text]
  • Threatened Fish : Cottus Petiti Bǎcescu & Bǎcescu-Meşter, 1964 (Cottidae)
    OATAO is an open access repository that collects the work of Toulouse researchers and makes it freely available over the web where possible This is a Publisher’s version published in: http://oatao.univ-toulouse.fr/25523 Official URL: https://doi.org/10.26028/cybium/2019-433-001 To cite this version: Lefebvre, Stephane and Richard, Sylvain and Beaudou, Dominique and Denys, Gaël Threatened fish : Cottus petiti Bǎcescu & Bǎcescu-Meşter, 1964 (Cottidae). (2019) Cybium, 43 (3). 215-216. ISSN 0399-0974 Any correspondence concerning this service should be sent to the repository administrator: [email protected] Threatened fish Cottus petiti Bǎcescu & Bǎcescu-Meşter, 1964 (Cottidae) CR by SFI © Stéphane LEFEBVRE* (1), Sylvain RICHARD (2), Dominique BEAUDOU (3) Submitted: 27 May 2019 Accepted: 24 Jul. 2019 & Gaël P.J. DENYS (4, 5) Editor: P. Keith Common name(s) Lez sculpin (English), Chabot du Lez (French) (Fig. 1). IUCN status Critically Endangered in France according to the IUCN guide- lines (UICN France, 2019). Identification It is the smallest French cottid species (max. 6.5 cm TL; Keith et al., 2011), with prickling on body, extending backwards to cau- dal peduncle in juveniles and adults; no distinct transverse bands on pelvic-fins; last dorsal-fin ray is almost reaching the caudal-fin rays; eye diameter 27 to 31% of the head length (HL); interorbital distance 1.9 to 2.4 times the eye diameter; last anal-fin ray connect- ed to the body by a membrane along about 2/3 of its length; and depth of the caudal peduncle 24 to 28% HL (Freyhof et al., 2005).
    [Show full text]
  • Etude De La Faune Piscicole : Frayères Potentielles
    RECONSTRUCTION DES BARRAGES MANUELS DE L’AISNE ET DE LA MEUSE Etude de la faune piscicole : frayères potentielles SOUS DOSSIER : BARRAGE DE VAUXROT Indice Suivi des modifications Rédaction Vérification Validation A Première édition A-C. MONNIER E.THIEBAUT A.PEDON-FLESH Date Nom du fichier Nb. de page (s) 07/10/2013 PEMA2013BarragesPPP_Aisne_Frayeres_Vauxrot 36 Pedon Environnement & Milieux Aquatiques SARL 3, rue Paul Michaux - 57000 METZ Tél./fax : 03.87.50.65.78 – [email protected] SIRET : 518 593 587 00018 RECONSTRUCTION DES BARRAGES MANUELS DE L’AISNE ET DE LA MEUSE Etude piscicole « frayères potentielles » Sous dossier : Barrage de Vauxrot SOMMAIRE 1. CONTEXTE DE L’ETUDE ................................................................................................................ 1 2. MATERIEL ET METHODES ............................................................................................................ 2 2.1 Recueil des données et préparation des prospections ........................................................... 2 2.2 Les espèces protégées prises en compte ............................................................................... 3 2.2.1 Liste des espèces protégées ciblées et statuts de protection ............................................. 3 2.2.2 Périodes de reproduction des espèces protégées prises en compte ................................. 4 2.2.3 Granulométrie des frayères des espèces protégées prises en compte .............................. 4 2.3 Les barrages concernés .........................................................................................................
    [Show full text]
  • Publications Depuis 1992
    ANNEE 2018 18.2 Grosjean, A.S., Vennin, E., Olivier, N., Caravaca, G., Thomazo, C., Fara, E., Escarguel, G., Bylund, K.G., Jenks, G.B., Stephen, D.A., Brayard, A., 2018 - Early Triassic environmental dynamics and microbial development during the Smithian–Spathian transition (Lower Weber Canyon, Utah, USA). Sedimentary Geology, 363 : 136–151. 18.1 Parreira de Castro, D.M., Dolédec, S., Callisto, M., 2018 - Land cover disturbance homogenizes aquatic insect functional structure in neotropical savanna streams. Ecological Indicators, Volume 84 : 573-582. 18/12/2017 1 ANNEE 2017 17.37 Amiot, R., Wang, X., Wang, S., Lécuyer, C., Mazin, J.M., Mo, J., Flandrois J.P., Fourel, F., Wang, X., Xu, X., Zhang, Z., Zhou, Z., 2017 - d18o-Derived incubation temperatures of oviraptorosaur eggs. Palaeontology : 1–15.17.22 Barbe, L., Jung, V., Prinzing, A., Bittebiere, A.K., Butenschoen, O., Mony, C., 2017 - Functionally dissimilar neighbors accelerate litter decomposition in two grass species. New Phytologist, 214 : 1092-1102. 17.22 Barbe, L., Jung, V., Prinzing, A., Bittebiere, A.K., Butenschoen, O., Mony, C., 2017 - Functionally dissimilar neighbors accelerate litter decomposition in two grass species. New Phytologist, 214 : 1092-1102.17.8 Bardon, C., Poly, F., Haichar, Z., Le Roux, X., Simon, L., Meiffren, G., Comte, G., Rouifed, S., Piola, F., 2017 - Biological denitrification inhibition (BDI) with procyanidins induces modification of root traits, growth and N status in Fallopia x bohemica. Soil Biology & Biochemistry 107 : 41-4917.3 Binet, P., Rouifed, S., Jassey, V.E.J., Toussaint, M.L., Chiapusio, G., 2017 - Experimental climate warming alters the relationship between fungal root symbiosis and Sphagnum litter phenolics in two peatland microhabitats.
    [Show full text]
  • European Red List of Freshwater Fishes Jörg Freyhof and Emma Brooks Published by the European Commission
    European Red List of Freshwater Fishes Jörg Freyhof and Emma Brooks Published by the European Commission This publication has been prepared by IUCN (International Union for Conservation of Nature). The designation of geographical entities in this book, and the presentation of the material, do not imply the expression of any opinion whatsoever on the part of the European Commission or IUCN concerning the legal status of any country, territory, or area, or of its authorities, or concerning the delimitation of its frontiers or boundaries. The views expressed in this publication do not necessarily reflect those of the European Commission or IUCN. Citation: Freyhof, J. and Brooks, E. 2011. European Red List of Freshwater Fishes. Luxembourg: Publications Office of the European Union. Design & Layout by: Tasamim Design - www.tasamim.net Printed by: The Colchester Print Group, United Kingdom Picture credits on cover page: The European Mudminnow (Umbra krameri) is a European endemic found in the Danube and Dniestr basin. Its population has declined by more than 30% in the past 10 years due to river regulation and drainage of wetlands for agriculture which resulted in a reduced number of backwaters. This species is therefore classed as Vulnerable. Photograph © Andreas Hartl. All photographs used in this publication remain the property of the original copyright holder (see individual captions for details). Photographs should not be reproduced or used in other contexts without written permission from the copyright holder. Available from: Publications Office of the European Union, http://bookshop.europa.eu IUCN Publications Services, www.iucn.org/publications A catalogue of IUCN publications is also available.
    [Show full text]