Genomic Assessment of Taxonomic Status of Central Texas Eurycea Salamanders This project was funded by the U.S. Fish and Wildlife Service, Section 6 grant TX E158-R-1, through Texas Parks and Wildlife Department contract #443022 Principal Investigator: David M. Hillis Department of Integrative Biology University of Texas Austin, TX 78712 Phone: 512-471-5792 E-mail:
[email protected] Co-Principal Investigator: David C. Cannatella Department of Integrative Biology University of Texas Austin, TX 78712 Phone: 512-232-4862 E-mail:
[email protected] Additional Personnel: Thomas J. Devitt Department of Integrative Biology University of Texas Austin, TX 78712 current address: Environmental Scientist Watershed Protection Department City of Austin Austin, Texas 78704 April M. Wright Department of Integrative Biology University of Texas Austin, TX 78712 Submitted August 31, 2015; Approved by USFWS September 4, 2015 Abstract We conducted phylogenetic analyses of all known described and undescribed species of central Texas neotenic Eurycea (based on analyses of 34,518 loci), and population STRUCTURE analyses of all known populations of E. tonkawae, E. naufragia, and E. chisholmensis (based on analyses of 75,296 loci). Phylogenetic analysis supported the monophyly and genetic distinctiveness of all described species in the group; in particular, E. tonkawae, E. naufragia, and E. chisholmensis from north of the Colorado River were each reciprocally monophyletic, significantly supported, and genetically distinct. Population STRUCTURE analyses of the species north of the Colorado River also supported the species boundaries between E. tonkawae, E. naufragia, and E. chisholmensis. A few populations that have been provisionally assigned to E. naufragia were determined here to belong to E.