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December Alert December 2019 Alert Items 1 - 148 of 148 (Display the 148 citations in PubMed) 1. HLA. 2019 Dec 31. doi: 10.1111/tan.13789. [Epub ahead of print] Peptide-MHC class I and class II tetramers: From flow to mass cytometry. Christophersen A1,2,3. Author information: 1. KG Jebsen Coeliac Disease Research Centre, University of Oslo, Oslo, Norway. 2. Institute of Clinical Medicine, University of Oslo, Norway. 3. Department of Immunology, University of Oslo, Oslo, Norway. Abstract To develop better vaccines and more targeted treatments for cancer and autoimmune disorders, the disease-specific T cells and their cognate antigens need to be better characterized. For more than two decades, peptide-major histocompatibility complex (pMHC) tetramers and flow cytometry have been the gold standard for detection of CD8+ and CD4+ T cells specific to antigens in the context of MHC class I and class II, respectively. Nonetheless, more recent studies combining such reagents with mass cytometry, i.e. cytometry by time of flight (CyTOF), have offered far more comprehensive profiling of antigen-specific T-cell responses. In addition, mass cytometry has enabled ex vivo screening of CD8+ T-cell reactivities against hundreds of MHC class I restricted candidate epitopes. MHC-class II molecules, on the other hand, have been challenging to combine with mass cytometry as they are more complex and bind with lower affinities to cognate T-cell receptors than MHC-class I molecules. In this review, I discuss how techniques originally developed to improve the staining capacity of pMHC tetramers in flow cytometry led to the successful combination of such reagents with mass cytometry. Especially, I will highlight very recent advances facilitating the combination with pMHC- class II tetramers. Together, these mass cytometry-based studies can help develop more targeted treatments for cancer and autoimmune disorders. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved. PMID: 31891448 Similar articles 2. Food Chem X. 2019 Dec 13;5:100075. doi: 10.1016/j.fochx.2019.100075. eCollection 2020 Mar 30. Potassium bicarbonate improves dough and cookie characteristics through influencing physicochemical and conformation properties of wheat gluten. Chen G1, Hu R1, Li Y1. Author information: 1. Department of Grain Science and Industry, Kansas State University, Manhattan, KS 66506 United States. Abstract Baking soda (NaHCO3) has critical technological functions in cookie products. Health concern on excessive sodium consumption is increasing; therefore, it is necessary to explore NaHCO3 alternatives, such as KHCO3, for bakery products. This study investigated the impact of KHCO3 on the technological behaviors of cookie dough and end-uses in comparison with control samples prepared with NaHCO3 and explore the changes of physicochemical and conformation properties of soft wheat gluten during the process. Dough rheological measurements demonstrated that addition of KHCO3 reduced dough stickiness, and adding KHCO3 achieved similar dough and baking performances as using NaHCO3, which were partially attributed to the decrease of gliadin to glutenin ratio, changes of secondary structure, and intensive aggregation of gluten by introducing KHCO3. Cookie sensory attributes were also not adversely affected by using KHCO3. Therefore, partially replacing NaHCO3 with KHCO3 in cookie products can be an effective approach for sodium reduction. © 2019 Published by Elsevier Ltd. PMCID: PMC6928305 PMID: 31891157 Similar articles Conflict of interest statement The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. 3. BMJ Case Rep. 2019 Dec 29;12(12). pii: e233226. doi: 10.1136/bcr-2019-233226. Pregnancy and coeliac disease. Boers K1, Vlasveld T2, van der Waart R3. Author information: 1. Gynaecology and Obstetrics, Haaglanden Medical Centre, Bronovo Hospital, Den Haag, Zuid- Holland, The Netherlands [email protected]. 2. Haematology, Haaglanden Medical Centre, Bronovo Hospital, Den Haag, Zuid-Holland, The Netherlands. 3. Gynaecology and Obstetrics, Haaglanden Medical Centre, Bronovo Hospital, Den Haag, Zuid- Holland, The Netherlands. Abstract Coeliac disease (CD) is a small bowel disorder known for its intestinal manifestations like diarrhoea and weight loss. Less known are the extraintestinal manifestations of CD like haematological abnormalities but also altered female reproduction and pregnancy outcomes. Especially, undiagnosed CD may lead to adverse reproductive outcomes such as intrauterine growth restriction, stillbirth and preterm birth. In diagnosed and treated CD, adverse pregnancy outcomes might be prevented. © BMJ Publishing Group Limited 2019. No commercial re-use. See rights and permissions. Published by BMJ. PMID: 31888907 Similar articles Conflict of interest statement Competing interests: None declared. 4. Medicina (Kaunas). 2019 Dec 26;56(1). pii: E9. doi: 10.3390/medicina56010009. Celiac Disease: A Common Unrecognized Health Problem with a Very Delayed Diagnosis. Rodrigo L1. Author information: 1. Gastroenterology Unit, Hospital Universitario Central de Asturias, 33011 Oviedo, Asturias, Spain. Abstract Celiac disease (CD) is a clinical entity of autoimmune nature, related to the presence of a permanent gluten intolerance that affects genetically predisposed individuals, producing a chronic inflammation process that usually occurs in the small bowel [...]. PMID: 31888055 Similar articles 5. Diagnostics (Basel). 2019 Dec 26;10(1). pii: E12. doi: 10.3390/diagnostics10010012. Setup of Quantitative PCR for Oral Neisseria spp. Evaluation in Celiac Disease Diagnosis. Esposito MV1,2, Nardelli C1,2,3, Granata I4, Pagliuca C1, D'Argenio V2,3,5, Russo I6, Guarracino MR4, Salvatore P1,2,3, Del Vecchio Blanco G7, Ciacci C6, Sacchetti L2,3. Author information: 1. Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, 80131 Naples, Italy. 2. Ceinge Biotecnologie Avanzate S. C. a R. L., 80131 Naples, Italy. 3. Task Force on Microbiome Studies, University of Naples Federico II, 80100 Naples, Italy. 4. LabGTP (Laboratory of Genomics, Transcriptomics and Proteomics), Institute for High Performance Computing and Networking (ICAR), National Research Council (CNR), 80131 Naples, Italy. 5. San Raffaele Open University, 00166 Rome, Italy. 6. Department of Medicine and Surgery, University of Salerno, 84084 Salerno, Italy. 7. Department of System Medicine, University of Rome Tor Vergata, 00133 Rome, Italy. Abstract Coeliac disease (CD) is a multifactorial autoimmune disorder and gut dysbiosis contributes to its pathogenesis. We previously profiled by 16S rRNA sequencing duodenal and oropharyngeal microbiomes in active CD (a-CD), gluten-free diet (GFD) patients, and controls (CO) and found significantly higher levels of Neisseria spp., with pro-inflammatory activities, in a-CD patients than in the other two groups. In this study, we developed a fast and simple qPCR-based method to evaluate the abundance of the oral Neisseria spp. and the diagnostic performances of the test in CD diagnosis. The Neisseria spp. abundances detected by quantitative PCR (qPCR) were: CO = 0.14, GFD = 0.15, a- CD = 2.08, showing a similar trend to those previously measured by next generation sequencing (NGS). In particular, Neisseria spp. values obtained by both methods were significantly higher (p < 0.001) in a-CD than in the other two groups GFD and CO-the latter almost overlapping. We calculated by ROC curve analysis the threshold of 1.12 ng/μL of Neisseria spp. to discriminate between CO+GFD and a-CD patients with 100% and 96.7% of diagnostic sensitivity and specificity, respectively. In conclusion, our data, if confirmed in other cohorts, suggest the q-PCR evaluation of oral Neisseria spp. could be a fast and simple method to assess CD-associated dysbiosis for diagnostic purposes. PMID: 31888008 Similar articles 6. Gastroenterol Res Pract. 2019 Dec 11;2019:6272098. doi: 10.1155/2019/6272098. eCollection 2019. Prevalance of Celiac Disease in Patients with Inflammatory Bowel Disease in Turkish Population. Bengi G1, Cıvak M2, Akarsu M1, Soytürk M1, Ellidokuz E1, Topalak Ö1, Akpınar H1. Author information: 1. Gastroenterology Unit, Department of Internal Medicine, Dokuz Eylül University, İzmir, Turkey. 2. Department of Internal Medicine, Dokuz Eylül University, İzmir, Turkey. Abstract Background: Celiac disease (CD) and inflammatory bowel disease (IBD) involve inflammation of the gastrointestinal lumen, which environmental, genetic, and immunological factors have a role in their pathogenesis. The prevalence of celiac disease in IBD ranges from 0% to 14%. In this study, our aim was to determine the prevalence of CD in IBD patients followed by us who are attending the hospital or outpatient clinic over a period of time of seven years. Methods: Seven hundred and fifty nine patients (425 M, 334 F, mean age: 46.75, 396 ulcerative colitis (UC), 363 Crohn's disease (CrD)) diagnosed and followed up for IBD between January 2009 and July 2016 were evaluated retrospectively, and clinical, demographic, laboratory, and endoscopic data were collected. Results: CD was investigated in 79 (%10.4) inflammatory bowel disease patients according to symptoms, and in 5.06% (n = 4) of them, we diagnosed CD. The most common indication for investigating for CD was iron deficiency anemia unreponsive to iron supplementation.
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