LABORATORY SAFETY October 2013
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The Food Poisoning Toxins of Bacillus Cereus
toxins Review The Food Poisoning Toxins of Bacillus cereus Richard Dietrich 1,†, Nadja Jessberger 1,*,†, Monika Ehling-Schulz 2 , Erwin Märtlbauer 1 and Per Einar Granum 3 1 Department of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig Maximilian University of Munich, Schönleutnerstr. 8, 85764 Oberschleißheim, Germany; [email protected] (R.D.); [email protected] (E.M.) 2 Department of Pathobiology, Functional Microbiology, Institute of Microbiology, University of Veterinary Medicine Vienna, 1210 Vienna, Austria; [email protected] 3 Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, P.O. Box 5003 NMBU, 1432 Ås, Norway; [email protected] * Correspondence: [email protected] † These authors have contributed equally to this work. Abstract: Bacillus cereus is a ubiquitous soil bacterium responsible for two types of food-associated gastrointestinal diseases. While the emetic type, a food intoxication, manifests in nausea and vomiting, food infections with enteropathogenic strains cause diarrhea and abdominal pain. Causative toxins are the cyclic dodecadepsipeptide cereulide, and the proteinaceous enterotoxins hemolysin BL (Hbl), nonhemolytic enterotoxin (Nhe) and cytotoxin K (CytK), respectively. This review covers the current knowledge on distribution and genetic organization of the toxin genes, as well as mechanisms of enterotoxin gene regulation and toxin secretion. In this context, the exceptionally high variability of toxin production between single strains is highlighted. In addition, the mode of action of the pore-forming enterotoxins and their effect on target cells is described in detail. The main focus of this review are the two tripartite enterotoxin complexes Hbl and Nhe, but the latest findings on cereulide and CytK are also presented, as well as methods for toxin detection, and the contribution of further putative virulence factors to the diarrheal disease. -
Laboratory Safety Manual
LABORATORY SAFETY MANUAL Environment, Health & Safety 1120 Estes Drive Extension CB# 1650 TABLE OF CONTENTS Section or Chapter Page Introduction i Emergency Telephone Numbers ii EHS – Scope of Service iii Condensed Laboratory Safety Information for New Research Personnel v Chapter 1 – Laboratory Safety at the University of North Carolina at Chapel Hill 1-1 Chapter 2 – Laboratory Safety Plan 2-1 Chapter 3 – General Safety Principles and Practices 3-1 Chapter 4 – Proper Storage of Chemicals in Laboratories 4-1 Chapter 5 – Protective Clothing and Equipment 5-1 Chapter 6 – Safe Handling of Chemicals 6-1 Chapter 7 – Highly Toxic Chemicals and Select Carcinogens 7-1 Chapter 8 – Reproductive Health 8-1 Chapter 9 – Controlled Substances 9-1 Chapter 10 – Fire Safety 10-1 Chapter 11 – Explosive and Reactive Chemical Hazards 11-1 Chapter 12 – Management of Laboratory Wastes 12-1 Chapter 13 – Safe Handling of Peroxidizable Compounds 13-1 Chapter 14 – Safe Handling of Laboratory Animals 14-1 Chapter 15 – Safe Handling of Biological Hazards 15-1 Chapter 16 – Biological Safety Cabinets 16-1 Chapter 17 – Laboratory Hoods 17-1 Chapter 18 – Safe Use of Nanomaterials 18-1 Revisions to Laboratory Safety Manual REV-1 Laboratory Safety Manual – the University of North Carolina at Chapel Hill INTRODUCTION This manual is a safety reference document for laboratory personnel at the University of North Carolina at Chapel Hill. The University’s Department of Environment, Health & Safety prepared this manual, followed by review and approval from both the University’s Laboratory and Chemical Safety Committee (LCSC) and the University Safety and Security Committee (USSC). -
General Listing Background Document for the Inorganic Chemical Listing Determination
GENERAL LISTING BACKGROUND DOCUMENT FOR THE INORGANIC CHEMICAL LISTING DETERMINATION August, 2000 U.S. ENVIRONMENTAL PROTECTION AGENCY ARIEL RIOS BUILDING 1200 PENNSYLVANIA AVENUE, N.W. WASHINGTON, D.C. 20460 TABLE OF CONTENTS Page LIST OF TABLES .............................................................ii LIST OF FIGURES ............................................................ii LIST OF APPENDICES .........................................................ii 1. INTRODUCTION .......................................................1 1.1 BACKGROUND ...................................................1 1.2 EXISTING INORGANIC CHEMICAL LISTINGS ........................2 1.3 OTHER EPA REGULATORY PROGRAMS AFFECTING THE INORGANIC CHEMICAL INDUSTRY ............................................3 2. INDUSTRY DESCRIPTION .........................................5 2.1 INDUSTRY PROFILE ..............................................5 2.2 INDUSTRY SECTORS .............................................5 2.2.1 Antimony Oxide ..............................................8 2.2.2 Barium Carbonate ............................................8 2.2.3 Boric Acid ..................................................8 2.2.4 Cadmium Pigments ............................................8 2.2.5 Inorganic Hydrogen Cyanide ....................................8 2.2.6 Phenyl Mercuric Acetate .......................................8 2.2.7 Dry Process Phosphoric Acid ....................................8 2.2.8 Phosphorous Pentasulfide .......................................8 -
Evaluation of the Individual and Combined Toxicity of Fumonisin Mycotoxins in Human Gastric Epithelial Cells
International Journal of Molecular Sciences Article Evaluation of the Individual and Combined Toxicity of Fumonisin Mycotoxins in Human Gastric Epithelial Cells Song Yu, Bingxuan Jia, Na Liu, Dianzhen Yu and Aibo Wu * SIBS-UGENT-SJTU Joint Laboratory of Mycotoxin Research, CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China; [email protected] (S.Y.); [email protected] (B.J.); [email protected] (N.L.); [email protected] (D.Y.) * Correspondence: [email protected]; Tel.: +86-21-54920716 Received: 23 July 2020; Accepted: 14 August 2020; Published: 18 August 2020 Abstract: Fumonisin contaminates food and feed extensively throughout the world, causing chronic and acute toxicity in human and animals. Currently, studies on the toxicology of fumonisins mainly focus on fumonisin B1 (FB1). Considering that FB1, fumonisin B2 (FB2) and fumonisin B3 (FB3) could coexist in food and feed, a study regarding a single toxin, FB1, may not completely reflect the toxicity of fumonisin. The gastrointestinal tract is usually exposed to these dietary toxins. In our study, the human gastric epithelial cell line (GES-1) was used as in vitro model to evaluate the toxicity of fumonisin. Firstly, we found that they could cause a decrease in cell viability, and increase in membrane leakage, cell death and the induction of expression of markers for endoplasmic reticulum (ER) stress. Their toxicity potency rank is FB1 > FB2 >> FB3. The results also showed that the synergistic effect appeared in the combinations of FB1 + FB2 and FB1 + FB3. -
CBS SOP: Liquid Nitrogen AODA
Liquid Nitrogen SOP Liquid Nitrogen SOP CBS-SOP-020-19 Effective: Mar 2019 Author: A. Holliss Edited: P. Scheffer Purpose To provide procedural guidance on the dispensing, transport, handling and disposal of liquid nitrogen. This document is to be used in addition to on the job training and not as a substitution. Scope All students and staff within CBS working with liquid nitrogen should be familiar with these procedures. Outside of normal working hours (weekdays 08:30 to 16:30) liquid nitrogen must not be dispensed from the pressurized storage vessel. If there is an absolute necessity for liquid nitrogen from the pressurized storage dewar at that time, this procedure must be carried out with the supervisor’s knowledge by two trained personnel working together to allow for the alarm to be raised if there is an incident/accident. The second ‘spotter’ person is to be in visual contact with the first ‘dispensing’ person but not so close to also be in danger. Definitions/Acronyms Cryogenic storage dewar – a specialized double-walled vacuum container used for storing cryogenic liquid and provides thermal insulation as the cryogenic liquid slowly boils away. Excessive pressure is released through an open top, vented cap or through a pressure relief valve to prevent the risk of explosion. Oxygen deficient atmosphere – an atmosphere with less than 19.5% oxygen by volume. Air normally contains approximately 21% oxygen. If gases other than oxygen are added or mixed with air, the oxygen concentration is reduced (diluted) and oxygen deficiency occurs. No human sense will give an indication of an oxygen-reduced atmosphere. -
User's Manual
User’s Manual Model 331 Temperature Controller Includes Coverage For: Model 331S and Model 331E Lake Shore Cryotronics, Inc. 575 McCorkle Blvd. Westerville, Ohio 43082-8888 USA E-mail addresses: [email protected] [email protected] Visit our website at: www.lakeshore.com Fax: (614) 891-1392 Telephone: (614) 891-2243 Methods and apparatus disclosed and described herein have been developed solely on company funds of Lake Shore Cryotronics, Inc. No government or other contractual support or relationship whatsoever has existed which in any way affects or mitigates proprietary rights of Lake Shore Cryotronics, Inc. in these developments. Methods and apparatus disclosed herein may be subject to U.S. Patents existing or applied for. Lake Shore Cryotronics, Inc. reserves the right to add, improve, modify, or withdraw functions, design modifications, or products at any time without notice. Lake Shore shall not be liable for errors contained herein or for incidental or consequential damages in connection with furnishing, performance, or use of this material. Revision: 1.9 P/N 119-031 14 May 2009 Lake Shore Model 331 Temperature Controller User’s Manual LIMITED WARRANTY STATEMENT LIMITED WARRANTY STATEMENT (Continued) WARRANTY PERIOD: ONE (1) YEAR 9. EXCEPT TO THE EXTENT ALLOWED BY APPLICABLE LAW, 1. Lake Shore warrants that this Lake Shore product (the THE TERMS OF THIS LIMITED WARRANTY STATEMENT DO “Product”) will be free from defects in materials and NOT EXCLUDE, RESTRICT OR MODIFY, AND ARE IN workmanship for the Warranty Period specified above (the ADDITION TO, THE MANDATORY STATUTORY RIGHTS “Warranty Period”). If Lake Shore receives notice of any such APPLICABLE TO THE SALE OF THE PRODUCT TO YOU. -
Single Kernel Analysis of Fumonisins and Other Fungal Metabolites In
Single kernel analysis of fumonisins and other fungal metabolites in maize from South African subsistence farmers Jesper Mølgaard Mogensen, Stine Mørcholdt Sørensen, Michael Sulyok, Liana van der Westhuizen, Gordon Shephard, Jens Christian Frisvad, Ulf Thrane, Rudolf Krska, Kristian Fog Nielsen To cite this version: Jesper Mølgaard Mogensen, Stine Mørcholdt Sørensen, Michael Sulyok, Liana van der Westhuizen, Gordon Shephard, et al.. Single kernel analysis of fumonisins and other fungal metabolites in maize from South African subsistence farmers. Food Additives and Contaminants, 2011, pp.1. 10.1080/19440049.2011.611823. hal-00744827 HAL Id: hal-00744827 https://hal.archives-ouvertes.fr/hal-00744827 Submitted on 24 Oct 2012 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Food Additives and Contaminants For Peer Review Only Single kernel analysis of fumonisins and other fungal metabolites in maize from South African subsistence farmers Journal: Food Additives and Contaminants Manuscript ID: TFAC-2011-122.R2 Manuscript Type: Original Research Paper Date -
Aflatoxin, Fumonisin and Shiga Toxin-Producing Escherichia Coli
Toxins 2013, 5, 1872-1895; doi:10.3390/toxins5101872 OPEN ACCESS toxins ISSN 2072-6651 www.mdpi.com/journal/toxins Article Aflatoxin, Fumonisin and Shiga Toxin-Producing Escherichia coli Infections in Calves and the Effectiveness of Celmanax®/Dairyman’s Choice™ Applications to Eliminate Morbidity and Mortality Losses Danica Baines 1,*, Mark Sumarah 2, Gretchen Kuldau 3, Jean Juba 4, Alberto Mazza 5 and Luke Masson 5 1 Lethbridge Research Centre, Agriculture and Agri-Food Canada, 5403 1 Avenue South, Lethbridge, AB T1J 4B1, Canada 2 Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON N5V 4T3, Canada; E-Mail: [email protected] 3 PENNSTATE, 321 Buckhout Laboratory, University Park, PA 16802, USA; E-Mail: [email protected] 4 PENNSTATE, Fusarium Research Center, 216 Buckhout Laboratory, University Park, PA 16802, USA; E-Mail: [email protected] 5 National Research Council of Canada, Montréal, QC H4P 2R2, Canada; E-Mails: [email protected] (A.M.); [email protected] (L.M.) * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +1-403-320-8690; Fax: +1-403-382-3156. Received: 9 September 2013; in revised form: 8 October 2013 / Accepted: 11 October 2013 / Published: 23 October 2013 Abstract: Mycotoxin mixtures are associated with Shiga toxin-producing Escherichia coli (STEC) infections in mature cattle. STEC are considered commensal bacteria in mature cattle suggesting that mycotoxins provide a mechanism that converts this bacterium to an opportunistic pathogen. In this study, we assessed the mycotoxin content of hemorrhaged mucosa in dairy calves during natural disease outbreaks, compared the virulence genes of the STECs, evaluated the effect of the mucosal mycotoxins on STEC toxin expression and evaluated a Celmanax®/Dairyman’s Choice™ application to alleviate disease. -
Fumonisin Content in Conventional Versus Bt Maize and Implications Of
Iowa State University Capstones, Theses and Graduate Theses and Dissertations Dissertations 2013 Fumonisin content in conventional versus Bt maize and implications of fumonisin contamination on fuel ethanol yield and DDGS quality Erin Louise Bowers Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/etd Part of the Agriculture Commons, and the Toxicology Commons Recommended Citation Bowers, Erin Louise, "Fumonisin content in conventional versus Bt maize and implications of fumonisin contamination on fuel ethanol yield and DDGS quality" (2013). Graduate Theses and Dissertations. 13596. https://lib.dr.iastate.edu/etd/13596 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Fumonisin content in conventional versus Bt maize and implications of fumonisin contamination on fuel ethanol yield and DDGS quality by Erin Louise Bowers A dissertation submitted to the graduate faculty in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Major: Toxicology Program of Study Committee: Gary P. Munkvold, Major Professor Patricia A. Murphy Richard L. Hellmich Steve M. Ensley Lawrence A. Johnson Felicia Wu Anthony L. Pometto III Iowa State University Ames, Iowa 2013 Copyright -
WO 2016/074683 Al 19 May 2016 (19.05.2016) W P O P C T
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2016/074683 Al 19 May 2016 (19.05.2016) W P O P C T (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12N 15/10 (2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (21) International Application Number: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, PCT/DK20 15/050343 DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (22) International Filing Date: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, 11 November 2015 ( 11. 1 1.2015) KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, (25) Filing Language: English PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, (26) Publication Language: English SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: PA 2014 00655 11 November 2014 ( 11. 1 1.2014) DK (84) Designated States (unless otherwise indicated, for every 62/077,933 11 November 2014 ( 11. 11.2014) US kind of regional protection available): ARIPO (BW, GH, 62/202,3 18 7 August 2015 (07.08.2015) US GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, (71) Applicant: LUNDORF PEDERSEN MATERIALS APS TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, [DK/DK]; Nordvej 16 B, Himmelev, DK-4000 Roskilde DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, (DK). -
Safe Storage, Handling, Use and Disposal Procedures of Compressed Gas Cylinders
Title: Safe Storage, Handling, Use and Disposal Procedures of Compressed Gas Cylinders Effective Date: November 2005 Revision Date: March 1, 2017 Issuing Authority: VP, Capital Projects & Facilities Responsible Officer: Director Environmental Health and Safety PURPOSE OF THE POLICY The purpose of this policy on the safe storage, handling and use of compressed gas cylinders is to provide best practice guidance for the use and storage of compressed gas cylinders. These procedures are to assist employees on information to minimize the hazards associated with this type of equipment. SCOPE OF THIS POLICY Serious fire, explosion or rupture accidents may result from the misuse or mishandling of compressed gas cylinders. Observance of the following rules will help control hazards in the storage, handling and use of compressed gas cylinders. WHO NEEDS TO KNOW THIS POLICY This policy applies to all New York University academic, commercial and residential facilities utilizing compressed gas cylinders. Responsibilities: Department of Environmental Health and Safety • Responsible for developing and updating the policy • Work with Facilities, Department Heads, and Lab Managers to ensure proper use and storage • Enforce compliance with all safety regulations and FDNY regulations Directors, Department Chairs, and Principal Investigators • Directors, Chairs, and PIs are responsible for enforcing this policy. • Periodically review and monitor the effectiveness of safe CGC use and storage. • Allocate the resources necessary for safe handling, use, and to monitor the program. Department Managers and Supervisors • Department Managers and Supervisors ensure staff working with compressed gas cylinders have the appropriate training and understand all aspects of safety associated with this equipment. • Assure the equipment associated with the movement, storage, and use of compressed gas cylinders is available and properly inspected before being used. -
The Migration of Arsenic and Lead in Surface Sediments at Three Kids Mine Henderson, Nevada
Publications (WR) Water Resources 12-1997 The Migration of arsenic and lead in surface sediments at Three Kids Mine Henderson, Nevada Douglas Brian Sims Follow this and additional works at: https://digitalscholarship.unlv.edu/water_pubs Part of the Biogeochemistry Commons, Environmental Health and Protection Commons, Environmental Indicators and Impact Assessment Commons, Environmental Monitoring Commons, Geology Commons, and the Soil Science Commons Repository Citation Sims, D. B. (1997). The Migration of arsenic and lead in surface sediments at Three Kids Mine Henderson, Nevada. Available at: https://digitalscholarship.unlv.edu/water_pubs/21 This Thesis is protected by copyright and/or related rights. It has been brought to you by Digital Scholarship@UNLV with permission from the rights-holder(s). You are free to use this Thesis in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s) directly, unless additional rights are indicated by a Creative Commons license in the record and/ or on the work itself. This Thesis has been accepted for inclusion in Publications (WR) by an authorized administrator of Digital Scholarship@UNLV. For more information, please contact [email protected]. THE MIGRATION OF ARSENIC AND LEAD IN SURFACE SEDIMENTS AT THREE KIDS MINE HENDERSON, NEVADA by Douglas Brian Sims ^ Bachelor of Arts University of Nevada, Las Vegas 1995 A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Water Resources Management r •:'•• 3 338 . Department of Geoscience University of Nevada, Las Vegas December 1997 The Thesis of Douglas B.