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Anti-DIAPH1 Antibody (ARG55315)

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Anti-DIAPH1 Antibody (ARG55315) Product datasheet [email protected] ARG55315 Package: 100 μl anti-DIAPH1 antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes DIAPH1 Tested Reactivity Hu, Ms, Rat Tested Application IHC-P, WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name DIAPH1 Antigen Species Human Immunogen Recombinant protein of Human DIAPH1 Conjugation Un-conjugated Alternate Names DRF1; Diaphanous-related formin-1; LFHL1; DFNA1; Protein diaphanous homolog 1; hDIA1; DIA1 Application Instructions Application table Application Dilution IHC-P 1:50 - 1:200 WB 1:500 - 1:2000 Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Positive Control Mouse lung and HepG2 Calculated Mw 141 kDa Properties Form Liquid Purification Affinity purification with immunogen. Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol Preservative 0.02% Sodium azide Stabilizer 50% Glycerol Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. Note For laboratory research only, not for drug, diagnostic or other use. www.arigobio.com 1/2 Bioinformation Database links GeneID: 1729 Human Swiss-port # O60610 Human Gene Symbol DIAPH1 Gene Full Name diaphanous-related formin 1 Background This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. [UniProt] Research Area Signaling Transduction antibody Images ARG55315 anti-DIAPH1 antibody IHC-P image Immunohistochemistry: Paraffin-embedded Rat kidney stained with ARG55315 anti-DIAPH1 antibody at 1:100 dilution. ARG55315 anti-DIAPH1 antibody WB image Western blot: Mouse lung and HepG2 cell lysates stained with ARG55315 anti-DIAPH1 antibody. www.arigobio.com 2/2 Powered by TCPDF (www.tcpdf.org).
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