Anti-DIAPH1 Antibody (ARG55315)

Product datasheet [email protected]

ARG55315 Package: 100 μl anti-DIAPH1 antibody Store at: -20°C

Summary

Product Description Rabbit Polyclonal antibody recognizes DIAPH1

Tested Reactivity Hu, Ms, Rat

Tested Application IHC-P, WB

Host Rabbit

Clonality Polyclonal

Isotype IgG

Target Name DIAPH1

Antigen Species Human

Immunogen Recombinant protein of Human DIAPH1

Conjugation Un-conjugated

Alternate Names DRF1; Diaphanous-related formin-1; LFHL1; DFNA1; Protein diaphanous homolog 1; hDIA1; DIA1

Application Instructions

Application table Application Dilution

IHC-P 1:50 - 1:200

WB 1:500 - 1:2000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control Mouse lung and HepG2

Calculated Mw 141 kDa

Properties

Form Liquid

Purification Affinity purification with immunogen.

Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol

Preservative 0.02% Sodium azide

Stabilizer 50% Glycerol

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

www.arigobio.com 1/2 Bioinformation

Database links GeneID: 1729 Human

Swiss-port # O60610 Human

Gene Symbol DIAPH1

Gene Full Name diaphanous-related formin 1

Background This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. [UniProt]

Research Area Signaling Transduction antibody

Images

ARG55315 anti-DIAPH1 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Rat kidney stained with ARG55315 anti-DIAPH1 antibody at 1:100 dilution.

ARG55315 anti-DIAPH1 antibody WB image

Western blot: Mouse lung and HepG2 cell lysates stained with ARG55315 anti-DIAPH1 antibody.