Proceedings of the 1St Liaquat University of Medical & Health Sciences (LUMHS) International Medical Research Conference

Eur J Med Res 2017, 22(Suppl 1):53 https://doi.org/10.1186/s40001-017-0296-3 European Journal of Medical Research MEETING ABSTRACTS Open Access Proceedings of the 1st Liaquat University of Medical & Health Sciences (LUMHS) International Medical Research Conference Jamshoro, Pakistan. 17th - 18th December 2016 Published: 28 December 2017 Speaker presentations for this proposal we demonstrated that an increase in the intracellular . level of O2− led to the regulation of two important regulators of tumor survival and development, the survival kinase Akt and the Na /H S1 + + Molecular genetics and functional studies of hearing impairment exchanger NHE-1. More recently, analysis of breast carcinomas from in Pakistani population The Cancer Genome Atlas (TCGA) database revealed strong positive Mohsin Iqbal correlation between tumors’epithelial to mesenchymal (EMT) score Shaheed Zulfqar Ali Bhutto Medical University, Islamabad, Pakistan and the expression of O2·− regulator MnSOD. This positive correlation Correspondence: Mohsin Iqbal between MnSOD and EMT score was signifcant and consistent across European Journal of Medical Research 2017, 22(Suppl 1):S1 all breast cancer subtypes. Interestingly, using phenotypically distinct breast cancer cell lines, we provided evidence that constitutively high Hereditary hearing impairment is one of the common monogenic or induced expression of MnSOD promotes the EMT-like phenotype disorder in Pakistan. It is estimated that around 0.16% peoples have by way of a redox milieu predominantly driven by hydrogen peroxide moderate to profound prelingual hearing loss while one-third of peo- (H2O2). Conversely, gene knockdown of MnSOD results in the reversal ple above 60 years have some sort hearing problem. I had enrolled 150 of EMT to a MET-like program, which appears to be a function of super- families with prelingual hearing loss. Initial screening of these families oxide (O2·−)-directed signaling. These data underscore the involvement was done by genotyping reported loci with fuorescently labelled STR of MnSOD in the regulation of the switch between the EMT and MET- markers. Families showing linkage with specifc loci was confrmed by associated phenotype by infuencing cellular redox environment via NGS based platforms like OtoSeqand Sanger sequencing. Combina- its efect on the intracellular ratio of O2·− and H2O2. My address I will tion of these techniques led towards the identifcation. Combination present these new fndings and discus their importance for the man- of these techniques led towards the identifcation of 34 pathogenic agement of breast cancer. variants in GJB2, MYO7A, CDH23, MARVELD2 and POU3F4 in 40 families. Fifteen of these variants have not reported earlier. Families remain S3 unlinked with reported loci were subjected for Genome wide scan, Recent advances in the feld of cardiology SNP analysis and exome sequencing. A novel deafness locus DFNB79 Muhammad Jafer Ansari were mapped though GWS and three Novel gene GIPC3, TAPRN and Washington University, Barnes Jewish Health Care, Memorial Hospital, NARS2 were identifed by the combination of Linkage analysis and Belleville, IL, USA next generation exome sequencing. Using NGS based platforms like Correspondence: Muhammad Jafer Ansari OtoSeq, GWS, SNP analysis and exome sequencing in families segre- European Journal of Medical Research 2017, 22(Suppl 1):S3 gating hearing loss, will contribute to the identifcation of common and population specifc mutations, early diagnosis, genetic counseling I will discuss recent advances in the feld of cardiology, and how the and molecular epidemiology. management of the patients has changed in the last decade. What are the recent advances in Interventional Cardiology? What is the future of S2 research in Cardiology? What are the three most important advances Superoxide and carcinogenesis: my 20 years’ obsession in cardiology in the recent years which has impacted and revolution- Marie‑Veronique Clement‑Pervaiz ized our clinical practice? How can USA contribute to improve the Department of Biochemistry, Yong Loo Lin School of Medicine, NUS Grad‑ quality of research in Pakistan? How can technology help to improve uate School for Integrative Science and Engineering, National University the quality of care and research at LUMHS. I will give my suggestions of Singapore, Singapore to promote research and academic activities in the feld of cardiol- Correspondence: Marie‑Veronique Clement‑Pervaiz ogy and medicine. What is the real unmet clinical need in cardiology European Journal of Medical Research 2017, 22(Suppl 1):S2 for Pakistan? How can we focus the research for the improvement of quality of care in the right areas? We need ground breaking changes Over the years work of our group dispelled the dogmatic view of reac- which can impact community, not just papers to publish. What are the tive oxygen species as only toxic molecules by providing evidence for challenges in inculcating the most advanced technology and state of their involvement in cell survival signaling pertinent to carcinogenesis. the art therapies in Pakistan? I will fnally discuss how to handle the fnancial, logistical, and procedural hurdles for research and practice of Together our fndings led us to propose superoxide (O2·−) as the key oxygen species involved in the process of carcinogenesis. In support medicine in Sindh/Pakistan. © The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Eur J Med Res 2017, 22(Suppl 1):53 Page 2 of 20 S4 S6 Redox regulation of cancer cell fate decisions Transforming oral health academics & health care in Pakistan Shazib Pervaiz1,2 via introducing forensic odontology 1Department of Physiology, Yong Loo Lin School of Medicine and NUS Shahjahan Katpar Graduate School for Integrative Sciences and Engineering, National Institute of Dentistry, LUMHS, Jamshoro, Pakistan University of Singapore, Singapore; 2National University Cancer Institute, Correspondence: Shahjahan Katpar NUHS, Singapore European Journal of Medical Research 2017, 22(Suppl 1):S6 Correspondence: Shazib Pervaiz European Journal of Medical Research 2017, 22(Suppl 1):S4 Forensic Odontology or Forensic Dentistry is an important and well established Dental Specialty in the Advanced World and its formal The primary focus of our program over the years has been to investi- teaching has existed for more than a century. Time has come for this to gate signaling networks that promote cellular transformation and elu- be introduced in Pakistan for all its Academic, Research and Practical cidate mechanisms that endow cancer cells the ability to evade death reasons pertaining to Justice System & Crime. Its signifcance in terms execution. As such, research eforts have been dedicated to identify- of reporting, sharing scientifc evidence, handling Crime is infuenced ing potential bottlenecks or vulnerabilities that cut across a spectrum by the people who are properly Qualifed and trained as Forensic of human cancers, including hematopoietic malignancies and solid Odontologists and unfortunately we have none. As our society is rap- tumors involving the breast, colon, lung and pancreas. To that end, idly changing, becoming more commercial & losing its cultural, ethi- using a variety of model systems such as oncogene addiction-induced cal & human touch, therefore we feel it is high time for this neglected proliferation, overexpression of proteins that block apoptosis execu- Dental Specialty to be given birth-introduced as we aim for National tion, pharmacological inhibition of key cellular metabolic regulators, Paradigm Shift and share this Presentation. receptor and chemotherapy-induced death stimuli, and novel small Keywords: Teeth, Oral cavity, Forensic odontology, Dentistry, Facial molecule compounds, we provide evidence that cellular redox metab- xrays, Trauma, Assault. olism critically impacts cell fate decisions. Of note, across a wide spectrum of cellular redox stress, there emerges a dichotomy of responses S7 in terms of cell survival/proliferation and cell death. At the lower end Science in Muslim world: where do we stand? of the scale cell survival and proliferation is favored, while at the other Sultan Ayoub Meo extreme cell execution is the preferred outcome. How these varied College of Medicine, King Saud University, Riyadh, Saudi Arabia levels of stress evoke disparate biological responses with distinct Correspondence: Sultan Ayoub Meo functional outcome(s) and the signaling networks and potential cel- European Journal of Medical Research 2017, 22(Suppl 1):S7 lular targets that could be amenable to redox modifcation(s) are the major focus of our ongoing and future investigations. A summary of Muslim world covers an enormous geographic area, comprising of 57 our key fndings and ongoing/future studies as well as their relevance countries with 1.82 billion people. Muslim scientists have a proud his- and application to unraveling the signaling nodes and Achilles heel of tory of science during the period 750–1250 AD. The

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