Exhibit 4 APPENDIX E Benthic Assessment Report

Vermont Green Line Devco, LLC Exhibit 4: Environmental Effects Vermont Green Line Project Article VII Application

Benthic Macroinvertebrate Sample Analysis Report

Vermont Green Line Project Lake Champlain Vermont and New York

PREPARED FOR: TRC Companies, Inc. 124 Grove Street Franklin, Massachusetts 02038

PREPARED BY: ESS Group, Inc. 10 Hemingway Drive, 2nd Floor East Providence, Rhode Island 02915

Project No. T332-000

Revised January 19, 2016

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BENTHIC MACROINVERTEBRATE SAMPLE ANALYSIS REPORT Vermont Green Line Project Vermont and New York

Prepared For:

TRC Companies, Inc. 124 Grove Street Franklin, Massachusetts 02038

Prepared By:

ESS Group, Inc. 10 Hemingway Drive, 2nd Floor East Providence, Rhode Island 02915

Project No. T332-000

Revised January 19, 2016

© 2016 ESS Group, Inc. – This document or any part may not be reproduced or transmitted in any form or by any means, electronic, or mechanical, including photocopying, microfilming, and recording without the express written consent of ESS Group, Inc. All rights reserved.

TABLE OF CONTENTS

SECTION PAGE 1.0 INTRODUCTION ...... 1 2.0 METHODS ...... 1 2.1 Laboratory Analysis ...... 1 2.2 Data Analysis ...... 2 3.0 RESULTS ...... 2 3.1 Taxa Richness ...... 2 3.2 Macrofaunal Density ...... 3 3.3 Macrofaunal Community Composition ...... 3 3.4 Quality Assurance/Quality Control...... 7 3.5 Summary of Results ...... 7 4.0 REFERENCES ...... 7

TABLES

Table A Summary of Key Statistics from the Benthic Sample Analysis Table B Relative Abundance of Taxa Encountered Table C Most Widespread Taxa Encountered

FIGURES

Figure 1 Taxa Richness by Sample Figure 2 Total Macrofaunal Density by Sample Figure 3 Zebra Mussel Density by Sample Figure 4 Relative Abundance of Stylodrilus heringianus by Sample

APPENDICES

Appendix A Chains of Custody Appendix B List of Selected Taxonomic References Appendix C Benthic Sample Taxonomy and Enumeration Results

1.0 INTRODUCTION On behalf of TRC Companies, Inc. (the Client), ESS Group, Inc. (ESS) analyzed 30 samples collected from benthic habitats within the Lake Champlain portion of the Vermont Green Line Project cable route. Benthic macroinvertebrates were the primary target of the analysis and are defined as organisms greater than 500 microns (μm) in length that either live on or in aquatic sediments, including mollusks, primitive (unsegmented) worms, annelids (segmented worms), crustaceans and insects. 2.0 METHODS 2.1 Laboratory Analysis ESS received a total of 30 samples from the Client, including 20 samples on November 20, 2015 and 10 samples on December 2, 2015 (Appendix A). All samples originated from Vermont and New York waters of Lake Champlain. Samples were collected with a 0.1 m2 modified Day grab sampler, then field-sieved and fixed on board the survey vessel. The sieve mesh size was 0.5-mm and 10% neutral buffered formalin was used as the field fixative solution. Upon receipt at the laboratory, benthic samples were logged and checked for adequate preservation. Prior to sorting, sample material from each sample was emptied in its entirety into a 0.5-mm mesh sieve. Tap water was gently run over the sieve to rinse away any additional fine sediment that was not removed during the field sieving process as well as to remove the formalin solution prior to the microscope work. Rinsed samples were preserved in 70% ethanol. Each benthic sample was sorted to remove benthic organisms from residual debris. Samples with a high volume of sample debris were sub-sampled. Sub-sampling was conducted using a randomized procedure, in which the material was first split into 32 equal fractions using a gridded tray. Each grid in the tray was assigned a unique number. Sample fractions (sub-samples) were then selected using a random number sheet and carefully removed from the tray and sorted in their entirety under a high-power dissecting microscope (up to 90X magnification) until a target of at least 100 organisms was retained. The unsorted and sorted fractions of each sample were retained separately and preserved in 70% ethanol. Samples with minimal material or with fewer than 100 organisms were sorted in their entirety (i.e., without sub-sampling). For quality assurance and control (QA/QC) purposes, a second qualified staff member (quality assurance officer) resorted 10% of the samples analyzed by each sorter to ensure organisms were being adequately retained. The quality assurance officer checked the sorted sample material for any remaining organisms and calculated an efficiency rating ( E ) using the following formula: n E = 100× a na + nb

Where na is the number of individuals originally sorted and verified as identifiable organisms by the QC checker and nb is the number of organisms recovered by the QC checker. If the original sorter achieved E < 90% (i.e., less than 90% of the organisms in the sample removed), an additional sample sorted by that analyst was re-examined by the quality assurance officer. All sorted organisms were subsequently identified by a qualified taxonomist to the lowest practicable taxonomic level using a dissecting microscope with magnification up to 90X and readily available taxonomic keys. Oligochaetes and non-biting midges (Chironomidae) were mounted in CMC-10 mounting media using methods consistent with those outlined in Epler (2001). Identification of slide-mounted organisms was conducted under a compound microscope with magnification to 1,000X. The primary taxonomic references used for macroinvertebrate identification are listed in Appendix B.

Enumerations of macroinvertebrates identified from each sample were recorded directly in an electronic spreadsheet. Prior to data summary, species abundances for each sample were standardize to number of individuals per square meter, taking into account the sampling equipment dimensions and sub-sampling effort. 2.2 Data Analysis Measures of benthic macrofaunal diversity, abundance, and community composition were selected to describe existing conditions. The rationale behind selection of each measure follows. Diversity (Taxa Richness) Taxa richness is the number of different taxa that are found within a given area or community and is widely accepted as a robust assessment measure of diversity (Magurran 2003). For this study, taxa richness is defined as the total number of unique taxa found in a sample. Abundance (Macrofaunal Density) Macrofaunal density is an estimate of the number of individuals per unit area. The density of benthic organisms responds to disturbance as mitigated by the tolerance (or preference) of a given organism to the particular source of disturbance. Density may vary substantially over small areas or short periods of time and should therefore be interpreted cautiously. For this study, macrofaunal density is expressed as the number of organisms per square meter. Community Composition Community composition is a multivariate measure identifying the different benthic taxa present and respective abundances of each taxon. This descriptive measure uses information regarding the taxa present, providing detail to complement and help interpret summary metrics of diversity and abundance. 3.0 RESULTS Results of the benthic sample analysis, including taxa richness, density, and community composition are presented in the following sections. 3.1 Taxa Richness

The total number of taxa identified from the samples examined was 36 (Table A). Taxa richness per sample ranged from 1 at multiple locations (B-10, B-12, B-14, B-18, B28, B-30, B-32, B-34, and B-39) to 19 taxa at B-1 (Figure 1 and Appendix C) with a mean taxa richness of 4 taxa per site (Table A).

Table A. Summary of Key Statistics from the Benthic Sample Analysis Statistic Value Number of Samples 30 Mean Density per Square Meter (±1 SD) 848 ± 2084 Mean Taxa Richness (±1 SD) 4 ± 5 Total Number of Taxa 36 Number of Taxa Observed by Taxonomic Group Insects 19 Mollusks 7 Oligochaete Worms 5 Crustaceans 2 Other 3 Percent of Total Abundance by Taxonomic Group

Statistic Value Insects 31% Mollusks 34% Oligochaetes Worms 20% Crustaceans 1% Other 16%

Figure 1. Taxa Richness by Sample 3.2 Macrofaunal Density The mean macrofaunal density for the analyzed samples was 848 individuals/m2 (Table A). The highest macrofaunal density (10,480 individuals/m2) was found at B-1, while macrofaunal density was lowest (10 individuals/m2) at B-12 and B-14 (Figure 2 and Appendix C). Of the 30 samples analyzed, 17 were characterized by densities of 100 individuals/m2 or less. 3.3 Macrofaunal Community Composition The benthic macrofaunal assemblage documented in the analyzed samples consisted of insects, mollusks, oligochaete worms, oligochaete worms, leeches, and nematode worms (Appendix C). No rare, threatened, or endangered species were encountered. The most speciose taxonomic group was insects, which contributed more than half of the taxa documented in the analyzed samples (Table A).

The taxonomic group with the highest density was mollusks, followed by insects and oligochaete worms (Table A).

10,480

Figure 2. Total Macrofaunal Density by Sample The most abundant taxa observed were zebra mussel (Dreissena polymorpha), nematode roundworms (Nematoda) and the non-biting midge Cladotanytarsus sp (Table B). Together, these taxa accounted for more than 50% of all individuals identified in this study. Zebra mussel is an aquatic invasive species that was first documented in Lake Champlain in 1993 and has since colonized available habitats throughout the lake (VT DEC 2016). In the samples where it was observed, zebra mussel occurred at an average density of 226/m2, although it reached densities as high as 3,291/m2 in sample B-53 (Figure 3 and Appendix C). Table B. Relative Abundance of Taxa Encountered* Relative Abundance Scientific Name Common Name (%) Dreissena polymorpha Zebra mussel 27 Nematoda Nematode roundworm 15 Cladotanytarsus sp. Non-biting midge 12 Stylodrilus heringianus Blackworm 9 Spirosperma ferox Tubificid worm 7

Relative Abundance Scientific Name Common Name (%) Sphaeriidae Immature fingernail clams 5 Dicrotendipes sp. Non-biting midge 4 Procladius sp. Non-biting midge 3 Tubificidae w/o capilliform chaetae Immature tubificid worms 3 Hexagenia limbata Hex mayfly 3 Tribelos jucundum Non-biting midge 2 Microtendipes sp. Non-biting midge 2 *Includes taxa accounting for at least 1% of total abundance

Figure 3. Zebra Mussel Density by Sample The most widespread taxon (i.e., observed in the most samples) was the blackworm Stylodrilus heringianus, which was observed in 19 samples (Table C). This species was frequently the only one present or was co-dominant with other oligochaete worm species in low-density samples (Figure 4 and Appendix C). S. heringianus was also the fourth most abundant organism observed overall.

Figure 4. Relative Abundance of Stylodrilus heringianus by Sample Other widely distributed taxa included nematode roundworms (16 samples), the tubificid worm Spirosperma ferox (13 samples), and immature tubificid worms without capilliform chaetae (12 samples, although this may represent more than one species). The faucet snail (Bithynia tentaculata), which is considered an aquatic invasive species in Lake Champlain, was documented in three samples, each of which also hosted the invasive zebra mussel. Table C. Most Widespread Taxa Encountered* Number of Samples Scientific Name Common Name Containing this Taxon Stylodrilus heringianus Blackworm 19 Nematoda Nematode roundworm 16 Spirosperma ferox Tubificid worm 13 Tubificidae without capilliform chaetae Immature tubificid worms 12 Sphaeriidae Immature fingernail clams 7 Dresissena polymorpha Zebra mussel 6 Cladotanytarsus sp. Non-biting midge 4 Hydrobiidae Hydrobiid mud snail 4 Oecetis sp. Longhorn sedge (caddisfly) 4

Number of Samples Scientific Name Common Name Containing this Taxon Bithynia tentaculata Faucet snail 3 Probezzia sp. Biting midge 3 Tribelos jucundum Non-biting midge 3 *Includes taxa observed in at least 10% of samples 3.4 Quality Assurance/Quality Control QA/QC sorting efficiency checks were conducted on four samples. Taxonomic identification and enumeration checks were conducted on three samples. All QA/QC sorting efficiency, taxonomic identification and enumeration criteria were met for this project. Identifications represent the lowest practicable taxonomic level, given the maturity and condition of the organisms encountered, as well as the current state of taxonomic consensus. The majority of organisms were successfully identified to genus/species level. 3.5 Summary of Results • Thirty-six aquatic invertebrate taxa were observed in the thirty samples analyzed for this project. • Taxa richness averaged four per site, although a majority of the samples (sixteen) contained two taxa or less. • Although the mean macroinvertebrate density was over 800 organisms/m2, a majority of the samples (17) presented densities of 100 organisms/m2 or less. • The benthic community in the analyzed samples consisted of aquatic insects, mollusks, oligochaete worms, oligochaete worms, leeches, and nematode worms. • No rare, threatened or endangered species were encountered in the analyzed samples. • Two aquatic invasive species were encountered in the analyzed samples. • The most speciose taxonomic group was insects, which contributed more than half of the taxa documented in the analyzed samples • The most abundant organism observed was the zebra mussel, an aquatic invasives species, although it was restricted to six samples. • The most widely distributed taxon observed was the blackworm Stylodrilus heringianus, which was observed in 19 samples, including several samples in which it was the only species present. 4.0 REFERENCES Epler, J.H. 2001. Identification Manual for the Larval Chironomidae (Diptera) of North and South Carolina. Version 1.0. Magurran, A.E. 2003. Measuring Biological Diversity. Malden, MA: Blackwell Publishing Ltd. Vermont Department of Environmental Conservation [VT DEC]. 2016. Lake Champlain Zebra Mussel Monitoring Program. Accessed online: http://www.watershedmanagement.vt.gov/lakes/htm/lp_lczebramon.htm

Appendix A

Chains of Custody

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Appendix B

List of Selected Taxonomic References

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Benthic Macroinvertebrate Sample Analysis Report – Vermont Green Line Project Appendix B. List of Taxonomic References

List of Useful Freshwater Macroinvertebrate Taxonomic References Beaty, S. R. 2011. The Ephemeroptera of North Carolina. A Biologist’s Handbook with Standard Taxonomic Effort Levels. Biological Assessment Unit, Division of Water Quality, North Carolina Department of Environment and Natural Resources. Bolton, M. J. 2012. Ohio EPA Supplemental Keys to the Larval Chironomidae (Diptera) of Ohio and Ohio Chironomidae Checklist. Ohio Environmental Protection Agency, Division of Surface Water. Bousfield, E. L. 1973. Shallow-water Gammaridean Amphipoda of New England. Ithaca, NY: Cornell University Press. Brinkhurst, R. O. 1986. Guide to the Freshwater Aquatic Microdrile Oligochaetes of North America. Canadian Special Publication of Fisheries and Aquatic Sciences 84. Ottawa: Department of Fisheries and Oceans. Epler, J. H. 2001. Identification manual for the larval Chironomidae (Diptera) of North and South Carolina. Special Publication SJ2001-SP13. North Carolina Department of Environment and Natural Resources and St. Johns River (Florida) Water Management District. Gelhaus, J. K. 2002. Manual for the Identification of Aquatic Crane Fly Larvae for Southeastern United States. Prepared for the Carolina Area Benthological Workshop, Durham, North Carolina. Philadelphia, PA: Academy of Natural Sciences. Jokinen, E. H. 1992. The Freshwater Snails (Mollusca: Gastropoda) of New York State. New York State Museum Bulletin No. 482. Jokinen, E. H. 1996. Freshwater Snail Workshop Key. New England Association of Environmental Biologists, March 1996. University of Connecticut Institute of Water Resources. Lugo-Ortiz, C. R. and W. P. McCafferty. 1998. A new North American genus of Baetidae (Ephemeroptera) and key to Baetis complex genera. Entomological News, 109(5):345-353. Merritt, R.W., K.W. Cummins, and M.B. Berg, eds. 2008. An Introduction to the Aquatic Insects of North America, 4th ed. Dubuque, IA: Kendall/Hunt Publishing Co. Myers, L. W., B. C. Kondratieff, T. B. Mihuc, and D. E. Ruiter. 2011. The Mayflies (Ephemeroptera), Stoneflies (Plecoptera), and Caddisflies (Trichoptera) of the Adirondack Park (New York State). Transactions of the American Entomological Society, 137(1+2): 63-140. Peckarsky, B. L., P. R. Fraissinet, M. A. Penton, and D. J. Conklin, Jr. 1990. Freshwater Macroinvertebrates of Northeastern North America. Ithaca, NY: Cornell University Press. Pfeiffer, J. E. Kosnicki, M. Bilger, B. Marshall, and W. Davis. 2008. Taxonomic Aids for Mid-Atlantic Benthic Macroinvertebrates. Ephemeroptera: Baetidae. Plecoptera: Capniidae/Leuctridae. Diptera: Simuliidae. U.S. Environmental Protection Agency, Office of Environmental Information. Simpson, K. W. and R. W. Bode. 1980. Common larvae of Chironomidae (Diptera) from New York State streams and rivers, with particular reference to the fauna of artificial substrates. Bulletin of the New York State Museum, 439: 1-105. Simpson, K. W., R. W. Bode, J. P. Fagnani, and D. M. DeNicola. 1984. The Freshwater Macrobenthos of the Main Channel, Hudson River. Part B. Biology, Taxonomy and Distribution of Resident Macrobenthic Species. Final Report to Hudson River Foundation, Grant No. 8/83A/39. Smith, D. G. 1995. Keys to the Freshwater Macroinvertebrates of Massachusetts, 2nd ed.

Smith, D. G. 2001. Pennak’s Freshwater Invertebrates of the United States, 4th ed. New York: John Wiley & Sons. Stewart, K. W. and B. P. Stark. 1988. Nymphs of North American Stonefly Genera (Plecoptera). Denton, TX: University of North Texas Press. Stimson, K. S., D. J. Klemm, and J. K. Hiltunen. 1982. A Guide to the Freshwater Tubificidae (Annelida: Clitellata: Oligochaeta) of North America. U.S. Environmental Protection Agency, Environmental Monitoring and Support Laboratory. Wiggins, G. B. 1996. Larvae of the North American Caddisfly Genera, 2nd ed. Toronto: University of Toronto Press.

Appendix C

Benthic Sample Taxonomy and Enumeration Results

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Benthic Macroinvertebrate Sample Analysis Report - Vermont Green Line Appendix C. Benthic Sample Taxonomy and Enumeration Results

Taxa Group Final ID B-1 B-2 B-3 B-4 B-6 Fraction Sorted 0.125 1 1 1 1 Mollusk Dresissena polymorpha 1040 0 20 0 0 Mollusk Sphaeriidae 80 20 10 0 30 Mollusk Bithynia tentaculata 0 0 0 0 0 Mollusk Gyraulus deflectus 0 0 0 0 0 Mollusk Hydrobiidae 80 0 0 0 0 Mollusk Physidae 80 0 0 0 0 Mollusk Valvata tricarinata 0 0 0 0 0 Crustacean Gammarus fasciatus 0 0 0 0 0 Crustacean Mysis diluviana 0 10 0 0 0 Insect Macronychus glabratus 0 0 0 0 0 Insect Cladotanytarsus 2880 0 0 0 0 Insect Clinotanypus 80 0 0 0 0 Insect Dicrotendipes 1040 0 0 0 0 Insect Microtendipes 0 0 0 0 0 Insect Orthocladiinae 0 0 0 0 0 Insect Parachironomus 0 0 0 0 0 Insect Procladius 880 0 0 0 0 Insect Tanytarsini 0 10 0 0 0 Insect Tribelos jucundum 400 0 0 0 0 Insect Probezzia 80 0 0 0 0 Insect Tipulidae 160 0 0 0 0 Insect Caenis 160 0 0 0 0 Insect Hexagenia limbata 640 0 0 0 0 Insect Sialis 0 0 0 0 0 Insect Lepidostoma 0 0 0 0 0 Insect Molanna 80 0 0 0 0 Insect Mystacides 80 0 0 0 0 Insect Oecetis 160 0 0 0 0 Nematode Worm Nematoda 1600 140 250 290 50 Leech Glossiphonia complanata 0 0 0 0 0 Leech Mooreobdella sp. 0 0 0 0 0 Oligochaete Worm Lumbricina 0 0 0 0 0 Oligochaete Worm Stylodrilus heringianus 0 30 0 70 200 Oligochaete Worm Spirosperma ferox 640 160 270 330 0 Oligochaete Worm Tubificidae w/o capilliform chaetae 320 0 40 0 50 Oligochaete Worm Tubificidae w/ capilliform chaetae 0 0 0 40 0 Taxa Richness 19 6 5 4 4 Total Density 10480 370 590 730 330 Note: Values represent organism density as individuals/m2, unless otherwise noted

Page 1 of 5 Benthic Macroinvertebrate Sample Analysis Report - Vermont Green Line Appendix C. Benthic Sample Taxonomy and Enumeration Results

Taxa Group Final ID B-8 B-10 B-12 B-14 B-16 B-18 Fraction Sorted 1 1 1 1 1 1 Mollusk Dresissena polymorpha 0 0 0 0 0 0 Mollusk Sphaeriidae 10 0 0 0 0 0 Mollusk Bithynia tentaculata 0 0 0 0 0 0 Mollusk Gyraulus deflectus 0 0 0 0 0 0 Mollusk Hydrobiidae 0 0 0 0 0 0 Mollusk Physidae 0 0 0 0 0 0 Mollusk Valvata tricarinata 0 0 0 0 0 0 Crustacean Gammarus fasciatus 0 0 0 0 0 0 Crustacean Mysis diluviana 0 0 0 0 0 0 Insect Macronychus glabratus 0 0 0 0 0 0 Insect Cladotanytarsus 0 0 0 0 0 0 Insect Clinotanypus 0 0 0 0 0 0 Insect Dicrotendipes 0 0 0 0 0 0 Insect Microtendipes 0 0 0 0 0 0 Insect Orthocladiinae 0 0 0 0 0 0 Insect Parachironomus 0 0 0 0 0 0 Insect Procladius 0 0 0 0 0 0 Insect Tanytarsini 0 0 0 0 0 0 Insect Tribelos jucundum 0 0 0 0 0 0 Insect Probezzia 0 0 0 0 0 0 Insect Tipulidae 0 0 0 0 0 0 Insect Caenis 0 0 0 0 0 0 Insect Hexagenia limbata 0 0 0 0 0 0 Insect Sialis 0 0 0 0 0 0 Insect Lepidostoma 0 0 0 0 0 0 Insect Molanna 0 0 0 0 0 0 Insect Mystacides 0 0 0 0 0 0 Insect Oecetis 0 0 0 0 0 0 Nematode Worm Nematoda 0 0 0 0 10 0 Leech Glossiphonia complanata 0 0 0 0 0 0 Leech Mooreobdella sp. 0 0 0 0 0 0 Oligochaete Worm Lumbricina 0 0 0 0 0 0 Oligochaete Worm Stylodrilus heringianus 0 0 10 10 20 30 Oligochaete Worm Spirosperma ferox 30 20 0 0 0 0 Oligochaete Worm Tubificidae w/o capilliform chaetae 0 0 0 0 0 0 Oligochaete Worm Tubificidae w/ capilliform chaetae 0 0 0 0 0 0 Taxa Richness 2 1 1 1 2 1 Total Density 40 20 10 10 30 30 Note: Values represent organism density as individuals/m2, unless otherwise noted

Page 2 of 5 Benthic Macroinvertebrate Sample Analysis Report - Vermont Green Line Appendix C. Benthic Sample Taxonomy and Enumeration Results

Taxa Group Final ID B-20 B-22 B-26 B-28 B-30 Fraction Sorted 1 1 1 1 1 Mollusk Dresissena polymorpha 0 0 0 0 0 Mollusk Sphaeriidae 0 0 0 0 0 Mollusk Bithynia tentaculata 0 0 0 0 0 Mollusk Gyraulus deflectus 0 0 0 0 0 Mollusk Hydrobiidae 0 0 0 0 0 Mollusk Physidae 0 0 0 0 0 Mollusk Valvata tricarinata 0 0 0 0 0 Crustacean Gammarus fasciatus 0 0 0 0 0 Crustacean Mysis diluviana 0 0 0 0 0 Insect Macronychus glabratus 0 0 0 0 0 Insect Cladotanytarsus 0 0 0 0 0 Insect Clinotanypus 0 0 0 0 0 Insect Dicrotendipes 0 0 0 0 0 Insect Microtendipes 0 0 0 0 0 Insect Orthocladiinae 0 0 0 0 0 Insect Parachironomus 0 0 0 0 0 Insect Procladius 0 0 0 0 0 Insect Tanytarsini 0 0 0 0 0 Insect Tribelos jucundum 0 0 0 0 0 Insect Probezzia 0 0 0 0 0 Insect Tipulidae 0 0 0 0 0 Insect Caenis 0 0 0 0 0 Insect Hexagenia limbata 0 0 0 0 0 Insect Sialis 0 0 0 0 0 Insect Lepidostoma 0 0 0 0 0 Insect Molanna 0 0 0 0 0 Insect Mystacides 0 0 0 0 0 Insect Oecetis 0 0 0 0 0 Nematode Worm Nematoda 0 0 150 0 0 Leech Glossiphonia complanata 0 0 0 0 0 Leech Mooreobdella sp. 0 0 0 0 0 Oligochaete Worm Lumbricina 0 0 0 0 0 Oligochaete Worm Stylodrilus heringianus 20 60 240 40 0 Oligochaete Worm Spirosperma ferox 10 0 0 0 0 Oligochaete Worm Tubificidae w/o capilliform chaetae 0 40 40 0 30 Oligochaete Worm Tubificidae w/ capilliform chaetae 0 0 0 0 0 Taxa Richness 2 2 3 1 1 Total Density 30 100 430 40 30 Note: Values represent organism density as individuals/m2, unless otherwise noted

Page 3 of 5 Benthic Macroinvertebrate Sample Analysis Report - Vermont Green Line Appendix C. Benthic Sample Taxonomy and Enumeration Results

Taxa Group Final ID B-32 B-34 B-36 B-39 B-42 Fraction Sorted 1 1 0.125 1 1 Mollusk Dresissena polymorpha 0 0 0 0 0 Mollusk Sphaeriidae 0 0 1040 0 0 Mollusk Bithynia tentaculata 0 0 0 0 0 Mollusk Gyraulus deflectus 0 0 0 0 0 Mollusk Hydrobiidae 0 0 0 0 0 Mollusk Physidae 0 0 0 0 0 Mollusk Valvata tricarinata 0 0 0 0 0 Crustacean Gammarus fasciatus 0 0 0 0 0 Crustacean Mysis diluviana 0 0 0 0 0 Insect Macronychus glabratus 0 0 0 0 0 Insect Cladotanytarsus 0 0 0 0 0 Insect Clinotanypus 0 0 0 0 0 Insect Dicrotendipes 0 0 0 0 0 Insect Microtendipes 0 0 0 0 0 Insect Orthocladiinae 0 0 0 0 0 Insect Parachironomus 0 0 0 0 0 Insect Procladius 0 0 0 0 0 Insect Tanytarsini 0 0 0 0 0 Insect Tribelos jucundum 0 0 0 0 0 Insect Probezzia 0 0 0 0 0 Insect Tipulidae 0 0 0 0 0 Insect Caenis 0 0 0 0 0 Insect Hexagenia limbata 0 0 0 0 0 Insect Sialis 0 0 0 0 0 Insect Lepidostoma 0 0 0 0 0 Insect Molanna 0 0 0 0 0 Insect Mystacides 0 0 0 0 0 Insect Oecetis 0 0 0 0 0 Nematode Worm Nematoda 0 0 0 0 10 Leech Glossiphonia complanata 0 0 0 0 0 Leech Mooreobdella sp. 0 0 0 0 0 Oligochaete Worm Lumbricina 0 0 0 0 0 Oligochaete Worm Stylodrilus heringianus 40 100 1120 40 0 Oligochaete Worm Spirosperma ferox 0 0 160 0 0 Oligochaete Worm Tubificidae w/o capilliform chaetae 0 0 160 0 10 Oligochaete Worm Tubificidae w/ capilliform chaetae 0 0 0 0 0 Taxa Richness 1 1 4 1 2 Total Density 40 100 2480 40 20 Note: Values represent organism density as individuals/m2, unless otherwise noted

Page 4 of 5 Benthic Macroinvertebrate Sample Analysis Report - Vermont Green Line Appendix C. Benthic Sample Taxonomy and Enumeration Results

Taxa Group Final ID B-46 B-47 B-48 B-49 B-50 B-51 B-52 B-53 BA-1 Fraction Sorted 1 1 1 1 1 1 0.75 0.21875 0.5 Mollusk Dresissena polymorpha 0 0 0 0 0 620 693 3291 1180 Mollusk Sphaeriidae 0 0 10 0 0 0 0 0 0 Mollusk Bithynia tentaculata 0 0 0 0 0 10 0 91 20 Mollusk Gyraulus deflectus 0 0 0 0 0 0 0 46 0 Mollusk Hydrobiidae 0 0 0 10 0 0 80 46 0 Mollusk Physidae 0 0 0 0 0 0 0 0 0 Mollusk Valvata tricarinata 0 0 0 0 0 0 67 0 0 Crustacean Gammarus fasciatus 0 0 0 0 0 0 27 0 120 Crustacean Mysis diluviana 0 0 0 0 0 0 0 0 0 Insect Macronychus glabratus 0 0 0 0 0 0 0 0 20 Insect Cladotanytarsus 0 0 0 0 0 0 27 46 120 Insect Clinotanypus 0 0 0 0 0 0 13 0 0 Insect Dicrotendipes 0 0 0 0 0 0 0 0 0 Insect Microtendipes 0 0 0 0 0 0 0 411 40 Insect Orthocladiinae 0 0 0 0 0 0 0 0 60 Insect Parachironomus 0 0 0 0 30 0 0 0 0 Insect Procladius 0 0 0 0 0 0 0 0 0 Insect Tanytarsini 0 0 0 0 0 10 0 0 0 Insect Tribelos jucundum 0 0 0 0 0 10 0 91 0 Insect Probezzia 0 0 0 0 0 0 40 46 0 Insect Tipulidae 0 0 0 0 0 0 0 0 0 Insect Caenis 0 0 0 0 0 0 0 0 20 Insect Hexagenia limbata 0 0 0 0 0 0 0 0 0 Insect Sialis 0 0 0 0 0 0 0 0 20 Insect Lepidostoma 0 0 0 0 0 0 0 0 40 Insect Molanna 0 0 0 0 0 0 0 0 0 Insect Mystacides 0 0 0 0 0 0 0 0 0 Insect Oecetis 0 0 0 0 0 0 27 46 20 Nematode Worm Nematoda 120 30 20 20 90 30 467 594 0 Leech Glossiphonia complanata 0 0 0 0 0 20 0 0 20 Leech Mooreobdella sp. 0 0 0 0 0 0 0 0 40 Oligochaete Worm Lumbricina 0 0 0 0 0 0 0 46 0 Oligochaete Worm Stylodrilus heringianus 30 10 0 0 30 0 0 0 100 Oligochaete Worm Spirosperma ferox 20 0 0 20 0 0 13 46 160 Oligochaete Worm Tubificidae w/o capilliform chaetae 0 0 0 0 0 30 27 46 20 Oligochaete Worm Tubificidae w/ capilliform chaetae 0 0 0 0 0 0 0 0 0 Taxa Richness 3 2 2 3 3 7 11 13 16 Total Density 170 40 30 50 150 730 1480 4846 2000 Note: Values represent organism density as individuals/m2, unless otherwise noted

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