Journal of Virology
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JOURNAL OF VIROLOGY VOLUME 11 * NUMBER 6 * JUNE 1973 EDITORIAL BOARD Robert R. Wagner, Editor-in-Chief (1977) University of Virginia School of Medicine, Charlottesville Myron Levine, Editor (1976) Norman P. Salzman, Editor (1975) School of Medicine, National Institute of Allergy and University of Michigan, Infectious Diseases, Ann Arbor Bethesda, Md. Dwight L. Anderson (1974) Angus Graham (1975) Bernard Moss (1974) David Baltimore (1975) D. MacDonald Green (1974) Elmer R. Pfefferkorn (1974) Marcel A. Baluda (1975) Klaus Hummeler (1973) Lennart Philipson (1973) Allan M. Campbell (1975) Paul J. Kaesberg (1973) Fred Rapp (1975) Purnell Choppin (1974) Albert S. Kaplan (1973) Bernard Roizman (1973) Eugene H. Cota-Robles (1973) Edwin D. Kilbourne (1973) Aaron J. Shatkin (1973) Vittorio Defendi (1973) David W. Kingsbury (1973) Robert W. Simpson (1973) Walter Doerfier (1974) Lloyd M. Kozloff (1976) Donald F. Summers (1973) Richard M. Franklin (1973) Margaret Lieb (1973) Howard M. Temin (1973) Robert M. Friedman (1973) Jean Lindenmann (1973) Peter Vogt (1973) Harold S. Ginsberg (1973) Royce Z. Lockart, Jr. (1975) John S. Wiberg (1973) Marc Girard (1974) Christopher K. Mathews (1973) Julius Youngner (1973) Robert A. Day, Managing Editor, 1913 I St., N. W., Washington, D.C. 20006 EX OFFICIO R. G. E. Murray, President (1972-1973) L. Leon Campbell, Vice-President (1972-1973) Donald E. Shay, Secretary T. J. Carski, Treasurer The Journal of Virology, a publication of the American to the ASM Publications Office, 1913 I St., N.W., Wash- Society for Microbiology, 1913 I St., N.W., Washington, D.C. ington, D.C. 20006 (area 202 833-9416). Correspondence from 20006, is devoted to the dissemination of fundamental ASM members relating to membership dues, member knowledge concerning viruses of bacteria, plants, and ani- subscriptions, changes of address, incorrect address, mals. Investigators are invited to submit reports of original incorrect journals, etc., should be directed to the Executive research in all areas of basic virology, including biochemis- Secretary, American Society for Microbiology, 1913 I St., try, biophysics, genetics, immunology, morphology, and N.W., Washington, D.C. 20006. Published monthly by the physiology. The Journal is issued monthly, two volumes per ASM at 428 E. Preston St., Baltimore, Md. 21202. year. The subscription price is $40 (foreign, $41) per year; single copies are $4.00 (foreign, $4.25). Members of the American Society for Microbiology may receive the Journal Second-class postage paid at Baltimore, Md. 21202, and as part of their dues. Correspondence relating to sub- at additional mailing offices scriptions, reprints, -defective copies, availability of back Made in the U.S.A. issues, lost or late proofs, disposition of submitted inanu- Copyright 0 1973, American Society for Microbiology. scripts, and general editorial matters should be directed All Rights Reserved. JOURNAL OF VIROLOGY INSTRUCTIONS TO AUTHORS Correspondence. Submit manuscripts in dupli- Only one weight of heading (paragraph lead-in) cate (original and one carbon) to ASM Publica- should be used within each section. tions office, 1913 I St., N.W., Washington, D.C. Abstract. An Abstract appears at the beginning 20006. of each paper in the Journal. The abstract should General policy. Any manuscript submitted must not exceed 250 words. be a report of unpublished original research, which Literature Cited. In the text references are cited is not being considered for publication elsewhere. by number. The Literature dited section should A manuscript accepted and published by the be typed in alphabetical order, by first author, and Journal must not be published again in any form numbered. Names of journals are abbreviated ac- without the consent of ASM. cording to Chemical Abstracts Service Source In- A charge of $25 per printed page is assessed for dex (American Chemical Society, 1970). Litera- publication in the Journal. Most institutions and ture citations should be restricted to closely granting agencies in the United States permit the pertinent papers. Citations of abstracts, theses, payment of publication charges as part of their "unpublished data, " "personal communication," general research support. It is recognized, how- and "in press" will not be accepted in the Litera- ever, that such funds are not always available to ture Cited, but may be used parenthetically in laboratories outside the United States. In such the text. cases, the cost of publication will be borne by the Tables. Each table should be typed on a sepa- American Society for Microbiology. Ability or in- rate page. The data should be arranged so that ability to pay the publication charge is not a factor columns of like material read down, not across. in the selection of manuscripts for publication. The headings should be sufficiently clear so that The "editorial style" of the Journal essentially the meaning of the data will be understandable follows the CBE Style Manual (3rd ed., AIBS, without reference to the text. Explanatory foot- 1972). Biochemical abbreviations and nomen- notes are permitted, but detailed descriptions of clature should essentially follow "Biochemical the experiments are not. The materials and meth- Nomenclature" in Handbook of Biochemistry ods used to gain the data should properly remain (2nd ed., 1970 H A. Sober, ed., The Chemicaf in the section of that name. Rubber Co., dleveland, p. A4-A24). Normally, Figures. A complete set of figures, preferably abbreviations (except those of standard units of glossy photographs, should accompany each of the measurement and symbols of the elements) should two copies of the manuscript. Each figure should be defined and introduced parenthetically at first be numbered and should include the name of the use in the text. Certain abbreviations will be ac- author, either in the margin or on the back (marked cepted without definition in the title, abstract, and lightly with a soft pencil). Graphs (submit as text. Among these abbreviations are: DNA (de- photographs) should be finished drawings not oxyribonucleic acid); RNA (ribonucleic acid); needing further artwork or type-setting. Ab- rRNA (ribosomal RNA); mRNA (messenger solutely no part of a graph should be typewritten RNA); tRNA (transfer RNA); AMP, ADP, ATP, (except the legend, which should be typed on a dAMP, dADP, dATP (for the respective 5' phos- separate page). All lettering should be done with phates of adenosine or the other nucleosides, using a lettering set. Most graphs will be reduced to one- appropriate letter symbols); 2'-AMP, 3'-AMP, column width, and all elements in the drawing 5'-AMP (the 2'-, 3'-, and 5'-, where needed for con- should be prepared to withstand this reduction. trast, phosphates of the nucleosides); DNase The legend of the figure should provide enough (deoxyribonuclease); RNase (ribonuclease); P information so that the figure is understandable (orthophosphate); PPi (pyrophosphate); mol wt without reference to the text. Experimental de- (molecular weight); UV (ultraviolet); PFU tails from Materials and Methods should not be (plaque-forming units); Tris (tris (hydroxy- repeated in figure legends. methyl)aminomethane); DEAE- (diethylamino- Notes. The accepted form for Notes is somewhat ethyl-); and EDTA (ethylenediaminetetraace- different from the foregoing. Contributors should tate). Genetic symbols should essentially follow consult a recent issue of the Journal for style. the recommendations of Demerec et al. (Genetics Notes should not exceed 500 words. The Abstract 54:61, 1966). Enzyme activity should be expressed should not exceed 25 words. in terms of international units (Enzyme Nomen- Nomenclature of viruses. Viruses named after a clature, Elsevier Publishing Co., 1965), and the EC disease should be written as separate words in number should be given parenthetically at first use lower-case roman, except when the name of the in the text. In expressing lengths, weights and disease is derived from a proper noun: herpes volumes, the prefixes nano (n) and pico (p) should simplex virus, measles virus, Newcastle disease be used instead of millimicro (miu) and micromicro virus, poliomyelitis virus, varicella-zoster virus, (,s,s). Express lengths in nanometers (nm; 10-9 m) vaccinia virus. or in micrometers (Jum; 10 m) instead -of milli- Most other viruses should be written as a single microns (m,u; 10-'im), microns (u; 10 m), or word in lower case: adenovirus, coxsackievirus, Angstroms (A; 10-10 m). Express parts per million cytomegalovirus, echovirus, herpesvirus, mengo- (ppm) as micrograms per milliliter (pg/ml), micro- virus, picornavirus, poliovirus, poxvirus. grams per gram (ug/g), or microliters per liter Copyright. Once a paper has been published in (pliters/liter), as appropriate. In general, measure- the Journal, which is a copyrighted publication, ments should be expressed in terms of standard the legal ownership of all parts of the paper, in- international metric units. The Journal reserves cluding the illustrations, has passed from the the privilege of editing manuscripts to make them author to the Journal. If the same author, or any conform with the adopted style. author, wishes to republish material previously Form of manuscript. All parts of the manuscript published in the Journal, he must first receive should be typed double-space or, preferably, written permission from ASM. triple-space. Most manuscripts can and should be Reprints. Reprints (in multiples of 100) will be divided into the following sections: Abstract, In- furnished contributors when ordered in advance. troduction, Materials and Methods, Results Dis- A table showing the cost of reprints, and an order cussion, Acknowledgments, and Literature dited. form, will be sent with the proof. AUTHOR INDEX VOLUME 11 Aaronson, Stuart A., 218, 893 Compans, Richard W., 441 Heine, J. W., 810 Aguet, Michel, 1004 Copps, T. P., 552, 1017 Hemphill, H. Ernest, 25, 372 Allen, Patton T., 709 Cordell-Stewart, B., 232 Henry, Patrick H., 655, 665 Allison, David P., 585 Crumpacker, Clyde S., 655, 665 Hewlett, Martinez, 150 Altaner, C., 177 Hirth, C., 694 Anderson, Dwight L., 9, 153, 756, Darnell, J. E., 953 Hlavayova, E., 177 806 Deeb, Samir S., 353 Horowitz, Marshall S., 544 Andrews, D.