BIODIVERSITAS ISSN: 1412-033X Volume 22, Number 2, February 2021 E-ISSN: 2085-4722 Pages: 794-802 DOI: 10.13057/biodiv/d220233 First genetic record and the phylogenetic relationship of Osteochilus spilurus (Cyprinidae: Labeoninae ) originating from Bangka and Belitung Islands, Indonesia ARDIANSYAH KURNIAWAN1,2,, ANIK M. HARIATI3, ANDI KURNIAWAN3, DEWA G.R. WIADNYA3 1Doctoral Program, Faculty of Fisheries and Marine Science, Universitas Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia. Tel.: +62-341-553512, Fax.: +62-341-557837, email:
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[email protected] 2Department of Aquaculture, Faculty of Fisheries, Agriculture and Biology, Universitas Bangka Belitung. Jl. Kampus Peradaban Balunijuk, Merawang Bangka 33172, Bangka Belitung Islands, Indonesia 3Faculty of Fisheries and Marine Science, Universitas Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia. Tel.: +62-341-553512, Fax.: +62-341-557837, email:
[email protected] Manuscript received: 30 November 2020. Revision accepted: 16 January 2021. Abstract. Kurniawan A, Hariati AM, Kurniawan A, Wiadnya DGR. 2021. First genetic record and the phylogenetic relationship of Osteochilus spilurus (Cyprinidae: Labeoninae) originating from Bangka and Belitung Islands, Indonesia. Biodiversitas 22: 794-802. Osteochilus spilurus is a freshwater fish from Southeast Asia. Species identification for this fish in Indonesia is still solely limited to morphological characteristics. Therefore it is highly important to validate using short DNA sequences that have been proven to be fast, effective, and accurate. Fish specimens were collected from Lenggang river in Belitung island, and Lebak river in Bangka island, Indonesia. Mitochondrial cytochrome b gene (cyt b, 408 bp) was amplified and sequenced. We collected fish specimens from Lenggang River in Belitung Island and Lebak River in Bangka Island, Indonesia.