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Essential Oil Composition of Rose Flowers from Karangpring Village Jurnal ILMU DASAR, Vol.20 No. 2, Juli 2019 : 67-74 67 Essential Oil Composition of Rose Flowers from Karangpring Village Jember District Extracted by Distillation and Enfleurage Komposisi Minyak Atsiri Bunga Mawar dari Desa Karangpring Kabupaten Jember yang diekstrak melalui Distilasi dan Enfleurasi Ika Oktavianawati1*), Nanda Letisya1, Priscillia Citra1, Dwi Purwita Utari1, I Nyoman Adi Winata1, Wuryanti Handayani1, Ari Satya Nugraha2 1Dept. Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember 2Faculty of Pharmacy, University of Jember *E-mail: [email protected] ABSTRACT Karangpring is one tourist destination villages in Jember district which is popular with beautiful large rose field areas. Therefore, this area grows to be a leading producer of rose flowers in Jember district. However, the bulky presence of these flowers made its price becomes lower in regular days. Local community only uses and sells these fresh flowers as the flower for funeral. The rose flower has a great potency to be explored as a source of rose essential oil production. To date, there is no previous research on studying rose flowers from Karangpring village for its potency on the essential oil production. In this research, rose flowers were subjected to be extracted of its essential oil using two extraction methods, distillation, and enfleurage. Hydrodistillation resulted two phases of distillates, above part formed a cloudy white phase as a normal essential oil extracted from plants, and the lower phase was an aqueous phase containing rose hydrosols. Both phases of these condensates were analyzed using GCMS. Data explained that above phase, with a yield oil of 0.07%, only contains long-chain hydrocarbons such as n-nonadecane, n-heptadecane, 9-nonadecene, and eicosane, while the lower phase only contains 2-phenylethyl alcohol. On the other hand, enfleurage of fresh rose flowers resulted in 0.06% oil yield. GCMS analysis of this oil shows that 2-phenylethyl alcohol, eugenol, and phenylacetic acid are three major compounds which take more than 85% of total rose absolute. The results show that enfleurage is a better method for extracting rose oil in better quality than using the distillation method, in term of the variety of volatile components. Meanwhile, hydrodistillation is still benefiting from producing rose water that is qualified as an industrial additive agent for food and cosmetic productions or even a new potent of agromedicine products. Keywords: rose, rose oil, rose water, rose absolute, distillation, enfleurage. INTRODUCTION from rose flowers which attracts the attention of the world is the production of rose Rose flower has an economic and social value oil,known as rose otto, in flavor and fragrance in the world, especially the Indonesian industry (Kovats, 1987). Since rose oil is one community. Some communities regularly use of the most expensive essential oil in the world the flower as cut roses for many special events, market today, we proposed the way on the such as birthday and wedding parties, also for diversification product of rose flower as funeral and cemetery flowers. Karangpring essential oil production in this research village is a leading producer and supplier for (Agaoglu, 2000; Kovacheva et al. 2010). rose flowers in Jember district (Derap Desa, The main flavor component on the scent of 2010; Arifin, 2017b). Local communities in Karangpring village frequently use rose rose flowers are -damascone and - damascenone (Pal, 2013). Nevertheless, it is flowers only as formal flowers for cemetery also known that characteristic odor of rose and funeral aim. Some also produce healthy flowers came from 2-phenylethyl alcohol labeled juice from rose flowers, or even as rose which being a principal component in rose candies, teas, and crackers (Arifin, 2017a). Others also use rose water in cooking as a water (Watanabe et al. 2001; Sakai et al. 2007; natural coloring agent (Saati et al. 2011), Sakai et al. 2008; Kumar et al. 2014; Gunes, 2005; Hirata et al. 2016). Previous researches flavoring agents on desserts and many meat explained that bioactive molecule of 2- dishes (Karizaki et al. 2016; Alalwan et al. phenylethyl alcohol inside rose flowers is in 2017). However, another valuable product Journal homepage: https://jurnal.unej.ac.id/index.php/JID 68 Essential Oil Composition of Rose Flowers … (Oktavianawati, dkk) the form of 2-phenylethyl-D-glucopyranoside dimethyl ketone, acetaldehyde, sulfides, (Eikani et al. 2005; Watanabe et al. 2002). ammonia and methanol (Soe’eib et al. 2016; However, most publications only mention the Muchtar et al. 2013; Yulianingsih et al. 2007; finding on aglycon part of it, i.e. 2- Oktavianawati et al. 2017). phenylethylalcohol. It was assumed that instead Various rose extracts depending on the way of the presence of -glucosidase inside the they were extracted, have their names. Rose oil plant (Watanabe et al. 2001; Sakai et al. 2008), is normally obtained from the upper phase of applying various pretreatment and extraction distillate from steam, steam-water, and methods could hydrolyze the glycoside as well hydrodistillation methods. Rose concrete is to produce 2-phenylethyl alcohol. obtained by solvent extraction of rose flowers Some efforts have been made to extract and has a smooth like semi-solid performance, rose oil with higher quality, meaning that it has while rose absolute is derived from rose flower a high content of rose alcohol, but low or even extraction using ethanol as solvent. Rose water free of ester, higher alkanes, and fat (Eikani et is collected from the lower phase of distillate al. 2005). Various methods to extract rose oil which also contains a valuable oxygenated are distillation (Najem et al. 2011; Hosni et al. compound which is responsible for the great 2010; Agarwal et al. 2005), solvent extraction odor of rose flowers (Ulusoy et al. 2009). (Rusanov et al. 2011; Watanabe et al. 2001; To date, there was no previous Kurkeuoglu and Baser, 2003), enfleurage investigation on exploring essential oil from (Yulianingsih et al. 2007), and supercritical rose flowers in Jember district. Then, this CO2 (Reverchon et al. 1997). Kumar et current paper will discuss the potency of rose al.(2016) have applied distillation of Damask essential oil production and compare the oil rose flowers with saline water containing 75 g quality, in terms of GCM analysis data, NaCl/kg of rose flowers to increase about 42% because of applying different extraction higher of rose oil content compared to methods. distillation in water only. But there is a special METHODS consideration on applying this method in a significant scale process due to the Materials adverseeffect of salt on damaging steel Fresh rose flowers were obtained from a rose farmer apparatus in a longer period. community, Sumber Mawar, in Karangpring village, Jember district, from May to July 2018. Rose Most of all, the use of hydrodistillation in plantsthat were used in this research has been kinds of literature is quite widespread for identified by Lembaga Ilmu Pengetahuan Indonesia extracting rose oil from fresh rose flowers. It (Indonesian Institute of Sciences) Balai Konservasi has been known that hydrodistillation product Tumbuhan Kebun Raya Purwodadi as Rosa hybrida market value is higher than other product from Hort. different extraction methods, by the fact that Extraction Procedures the total number of essential oil components in Hydrodistillation hydrodistillation is over (Pal 2013). Research The whole flower of roses was used in new by Karmous et al. (2004) mentioned that conditions and extracted by hydrodistillation method hydrodistillation has a drawback that the effect using a Clevenger apparatus for 4 hours. The of boiling floral material and water at the same condensate formed two phases of distillate. The time could lead a light burned odor in the final upper phase was collected as rose oil, while the product of essential oil. Since hydrodistillation lower phase was rose water. Rose oil was subjected is also quite easy and inexpensive, then this to further analysis using GCMS. Rose water was extracted using diethyl ether to obtain rose concrete method became economical for essential oil and then was run for GCMS analysis as well. production and suitable to be applied to the local community as a kind of home industry. Enfleurage Chasis (50x50x7) was smeared with 500 grams of Enfleurage also becomes a popular shortening (white butter). 200 grams of fresh rose extraction method to produce absolutely flowers were placed on the top of fat from because it applies a low temperature for shortening for five days. The flowers were changed extraction. Hence, enfleurage prevents the in every 24 hours and substituted with the new fresh hydrolysis of essential oil component during one in a similar weight. After five days, the fat extraction that can be occurred at high containing essential oil was extracted using alcohol temperature process, and creates an unpleasant in a ratio of 2:1. Then the mixture was kept in 0-5C odour because of the presence of acetic acid, during 24 hours. Filtration of this mixture results inpomade and extrait. Extrait was evaporated for its Jurnal ILMU DASAR, Vol.20 No. 2, Juli 2019 : 67-74 69 alcohol content which then produced a rose absolute. also known as stereoptanes, including n- Rose absolute was also analyzed for its volatile nonadecane, n-heptadecane, 9-nonadecene, and content using GCMS. eicosane. In this term, the form of rose oil as a Identification of Volatile Components wax-like material is possibly originating from Volatile compounds of the essential oil extracts were the protective coatings of flowers which then analyzed by GCMS-QP2010S Shimadzu with Rtx 5 makes the fat hardens and solidifies at room MS column (length 30 m; ID 0.25 m; film 0.25 um), temperature (Sterrett 1962). The presence of Helium gas as a carrier, EI 70 Ev. Column oven this stearopten guarantees the durability of the temp.70C, injector temp.
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