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Annual Conference 2019 Poster Book Annual Conference 2019 8-11 April, ICC Belfast, UK @MicrobioSoc #Microbio19 Global food security: the challenges for microbiology Presentations: Monday and Tuesday evening A001 PHYTOCHEMICAL AND ANTIBACTERIAL PROPERTY OF FINGER MILLET (Eleusine Coracana) ON SOME SELECTED CLINICAL BACTERIA JeremiaH Ogbeba Federal PolytecHniC BauCHi , BauCHi, Nigeria Abstract Finger millet in nortHern Nigeria was subjected to pHytoCHemiCal sCreening using standard proCedures. The agar well metHod was used to test tHe antibaCterial aCtivities of metHanoliC and aqeous (Combined) extraCts of tHe grain on Salmonella typhi, EsCHeriCHia Coli, Pseudomonas aeruginosa and StapHyloCoCCus aureus. The result of tHe antimiCrobial activity as indiCated by zone of inHibition ranged from 1-8mm for different extract ConCentrations. The finger millet extract sHowed zones of inHibition of 8mm against Pseudomonas aeruginosa at a ConCentration of 100mg/ml, 3mm at 50mg/ml and 2mm at 25mg/ml ConCentrations. The inHibition zones of EsCHeriCHia Coli at extract ConCentrations of 100mg/ml,50mg/ml,25mg/ml, 12.25mg/ml and 6.125mg/ml were 4mm, 3mm, 3mm, 6mm and 1mm respeCtively, and for StapHyloCoCCus aureus were 5mm,2mm, 1mm at 100mg/ml, 50mg/ml and 12.25mg/ml respeCtively. The zones of inHibition against all tHe tested isolates at 100mg/ml was not signifiCantly different from those of 50mg/ml (p=0.160), 25mg/ml (p=0.067) and 12.5mg/ml (p=0.160), but signifiCantly HigHer than 6.125mg/ml (p=0.05). AlthougH S. aureus and S. typHi also did not differ signifiCantly in their susCeptibility to the varying ConCentrations of the extraCt (p=0.157), but susCeptibility by S. typHi was significantly lower tHan tHose of E. Coli (p=0.007) and P. aeruginosa (p=0.015). The qualitative phytochemiCal analysis indiCated the presence tannin/pHenol, flavonoids, alkaloid, saponin, glyCosides, terpenoid and steroids in finger millet. THe quantitative phytochemiCal revealed total phenoliC Content (6.57 mg/100g) and total flavonoid Content (0.224 mg/100g). The overall results indiCate that finger millet are potent antimiCrobial preparations at least invitro and also have high nutritional value. Global food security: the challenges for microbiology Presentations: Monday and Tuesday evening A002 Chaga Mushroom (Inonotus obliquus) inhibits growth of both lung adenocarCinoma (A549) Cells and Aspergillus fumigatus Magda Al-Tuwaijri1, JeHane IbraHim2,3 1Faculty of Applied ScienCe - Umm Al-Qura University, MakkaH, Saudi Arabia. 2Adham university College - Umm Al- Qura University, MakkaH, Saudi Arabia. 3Faculty of ScienCe - Cairo University, Makkah, Saudi Arabia Abstract Lung tumors and infections remain the most Common leading Cause of mortality worldwide. Chaga mushroom (Inonotus obliquus) Has long been Considered the king of mediCinal musHrooms wHiCH Constitutes an inexHaustible source of active compounds wHiCH can affect tHe survival of tumor cells. It has been used widely for Centuries as a dietary supplement and tea. In tHis sense, tHe aim of tHis study was to investigate in vitro cytotoxic capaCity of water extraCts of I. obliquus (CHaga musHroom) against the Human lung CanCer A549 Cell line after 72 h incubation. In addition, the extraCts were sCreened for antifungal aCtivity on Aspergillus fumigatus speCies, a life-threatening Cause of invasive pulmonary aspergillosis. The CytotoxiC and the antimiCrobial effeCts were performed using the MTT assay and the minimum inHibitory ConCentration (MIC) test, respeCtively. Owing to tHe notiCeable effeCt on antiproliferation of Hot-water extraCts, espeCially tHose from I. obliquus, tHe extract Could be of great potential to be used as an alternative CanCer tHerapy. However, it was not proven to Have antifungal effect against A. fumigatus fungi. Global food security: the challenges for microbiology Presentations: Monday and Tuesday evening A003 NUTRITIONAL QUALITY AND MICROBIAL DENSITY OF SWEET POTATO FLOUR FORTIFIED WITH SOYBEAN AND CRAYFISH FLOURS Tesleem IBRAHIM Rufus Giwa PolyteChniC,, Owo, Nigeria Abstract Nutritional quality and miCrobial density of sweet potato based Complementary food fortified witH soybean and crayfish flours was investigated. THree different samples were produced from mixing sweet potato, soybean and crayfish at different formulation. Sample I, II, III are sweet potato: soybean: CrayfisH at ratio 80:15:5, 70:20:10 and 60:25:15 respeCtively. The formulated flours were analysed for proximate Composition and miCrobial density. The result of proximate Composition sHowed that the value of moisture Content ranged between 6.50 - 8.50%, wHile asH Content, Crude protein Crude fat, Crude fibre, CarboHydrate and energy value ranged from 5.50 – 9.50%, 25.10 – 28.50%, 4.20 – 6.20%, 0.13 – 0.27%, 47.73 – 56.27% and 363.42 – 370.48 Kcal/100g respectively. Its observed that as the level of inClusion of soybean and CrayfisH to sweet potato flour inCreases, there was inCrease in protein and Crude fibre Content but decrease in CarboHydrate Content. Also, tHe total bacteria Count, Coliform Count, yeast and mould Count ranged from 28-50 x 10-4, 18-37 x 10-4 and 40-62 x 10-4 ( CFU/g ) respeCtively wHiCH are within the reCommended limit value for miCrobial density in food. THerefore, sweet potato flour fortified with soybean and crayfish flour can be reCommended as weaning food to reduce the incidence of malnutrition in infants. Global food security: the challenges for microbiology Presentations: Monday and Tuesday evening A004 Detoxifying Potentials of Two Indigenous Adsorbents: Imarsil and ACtivated CHarCoal in tHe ReduCtion of Aflatoxin in vegetable oils consumed in Nigeria. Oni Eniola, Oluwafemi Flora, Kareem Sarafadeen, Omemu Adebukunolami Federal University of AgriCuture, Abeokuta, Nigeria Abstract Food Contamination witH aflatoxin is more prevalent in tropiCal regions wHere environmental Conditions suCH as HigH temperature and Humidity prevail, wHiCH favour tHe growtH of toxigeniC fungi and acCumulation of tHese toxins in food and feeds represent a major tHreat to Human and animal HealtH. In lieu of the previously known adsorbents, adsorption studies of Aflatoxin (AF) were performed using inexpensive, readily available and loCal adsorbents Imarsil and activated CHarCoal (AC). Fifteen edible oils were purChases from open markets in Nigeria and sCreened for aflatoxin using HigH PerformanCe Liquid Chromatography (HPLC). Thirteen out of the fifteen vegetable oil samples were positive to aflatoxin at the following ConCentration (172, 123, 195, 142, 46, 107, 96, 116, 22, 33, 228, 17 and 4) ng/kg while two had no deteCtable AF. At six different concentrations (0.5, 1, 1.5, 2, 2.5 and 3%) of Imarsil and activated CHarCoal (AC) with ContaCt time of 1,2 and 3Hours at room temperature (37°C), the aflatoxin-adsorbing Capabilities depend on the adsorbent Concentrations and ContaCt time. Imarsil demostrated 100 % adsorption effiCienCy witHin one hour. At AF Contamination rates of 96-228ng/kg, activated CHarCoal was not effeCtive while Imarsil had 100 % removal effiCiency within 3 hours witH a signifiCant reduCtion (p< 0.05) observed at the HigHest Contamination rate and adsorbent ConCentration. AC demonstrated very mild adsorption activity. Results from tHis study indiCated tHat Industrial inCorporation of Imarsil into tHe oil refining proCess would reduCe greatly tHe menaCe of aflatoxiCosis. HenCe, tHe use of Imarsil should be encouraged. Global food security: the challenges for microbiology Presentations: Monday and Tuesday evening A005 Starter Culture development using selected strains of Bacillus spp. AssoCiated witH “kantong” produCtion in GHana Felix KwasHie Madilo1, RiCHard Kwame Glover2, Lawer Terlabie3 1University of Ghana, ACCra, Ghana. 23International CounCil for ScienCe, Regional OffiCe for AfriCa, Pretoria, SoutH AfriCa. 3Kwame Nkrumah University of ScienCe and TecHnology, Kumasi, GHana Abstract TeCHnologiCal properties of Bacillus spp. involved in fermenting Ceiba pentandra seeds into “kantong” a condiment prepared and Consumed in the northern part of Ghana were invested so as to develop starter Cultures with the predominant speCies for CommerCial production of “kantong”. The Bacillus species wHiCH predominated 205 Bacillus strains isolated from 11 stages of “kantong” production includes: Bacillus amyloliquefaciens, B. safensis, B. altitudinis, B. thuringiensis, B. pumilus, B. megaterium, B. cereus, B. circulans, B. coagulans, B. firmus, B. subtilis, and B. licheniformis. These predominant species were assessed for some teCHnologiCal properties suCH as proliferation at different temperatures and pH; substrate utilization preferences including “kantong” formulated media (i.e. 48-hrs sample, dried pellet and the final product) and inHibitory activity against 12 selected patHogeniC and spoilage miCroorganisms. The strains proliferated at different temperatures between 10°C and 55°C and pH of 2 to 9. Substrate utilization preferenCes were Nutrient Agar witH 5-9% Sodium CHloride (NA/ NaCl C, and ordinary Nutrient BrotH [NB], Nutrient Agar (NA), Potato Dextrose Agar (PDA), Tryptone Soya Agar (TSA), MaCConkey Agar (MCA) and “kantong” formulated media. All strains exHibited inHibitory aCtivity against one or more pathogeniC and spoilage organisms. Salmonella typhimurium, staphylococcus aureus, E. faecium and Proteus vulgris were tHe most susCeptible indiCator miCroorganisms. Many strains qualified as potential Candidates for seleCtion and development as starter Cultures to be used in tHe large sCale CommerCial produCtion of “kantong” of Consistent
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