Teaching About the Unintended Consequences of Plastics Tamara
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Recent Advances in Biocatalysts Engineering for Polyethylene Terephthalate Plastic Waste Green Recycling
Environment International 145 (2020) 106144 Contents lists available at ScienceDirect Environment International journal homepage: www.elsevier.com/locate/envint Review article Recent advances in biocatalysts engineering for polyethylene terephthalate plastic waste green recycling Nadia A. Samak a,b,c,1, Yunpu Jia a,b,1, Moustafa M. Sharshar a,b, Tingzhen Mu a, Maohua Yang a, Sumit Peh a,b, Jianmin Xing a,b,* a CAS Key Laboratory of Green Process and Engineering & State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China b College of Chemical Engineering, University of Chinese Academy of Sciences, 19 A Yuquan Road, Beijing 100049, PR China c Processes Design and Development Department, Egyptian Petroleum Research Institute, Nasr City, 11727 Cairo, Egypt ARTICLE INFO ABSTRACT Handling Editor: Guo-ping Sheng The massive waste of poly(ethylene terephthalate) (PET) that ends up in the landfills and oceans and needs hundreds of years for degradation has attracted global concern. The poor stability and productivity of the Keywords: available PET biocatalysts hinder their industrial applications. Active PET biocatalysts can provide a promising Plastic waste avenue for PET bioconversion and recycling. Therefore, there is an urgent need to develop new strategies that Poly(ethylene terephthalate) could enhance the stability, catalytic activity, solubility, productivity, and re-usability of these PET biocatalysts Recycling under harsh conditions such as high temperatures, pH, and salinity. This has raised great attention in using Biocatalysts ’ Bioengineering bioengineering strategies to improve PET biocatalysts robustness and catalytic behavior. Herein, historical and forecasting data of plastic production and disposal were critically reviewed. -
Close Encounters
Environment International 140 (2020) 105792 Contents lists available at ScienceDirect Environment International journal homepage: www.elsevier.com/locate/envint Close encounters - microplastic availability to pelagic amphipods in sub- antarctic and antarctic surface waters T ⁎ Kirstie Jones-Williamsa,b, , Tamara Gallowayb, Matthew Colec, Gabriele Stowassera, Claire Waludaa, Clara Mannoa a British Antarctic Survey, High Cross, Madingley Road, Cambridge CB30ET, United Kingdom b University of Exeter, Streatham Campus, Northcote House, Exeter EX4 4QJ, United Kingdom c Plymouth Marine Laboratory, Prospect Place, Plymouth PL1 3DH, United Kingdom ARTICLE INFO ABSTRACT Handling Editor: Adrian Covaci This study investigated the distribution of plastic debris from the Atlantic portion of the Sub-Antarctic to the Keywords: Antarctic Peninsula. This region is home to some of the highest concentrations of zooplankton biomass but is also Microplastics threatened by increasing shipping traffic from fishing and the growing tourism market. Samples were collected Mespolastics using a surface-towed neuston net during the Austral summer 2018, aboard the RRS James Clark Ross. Using Encounter Rate Fourier Transform Infrared Spectrometry it was found that 45.6% of the plastic particles isolated from seawater Synthetic Fibres samples were sampling contamination, originating predominantly from the ship. Of the remaining particles, both Amphipods low density (polyethylene, polypropylene) and high-density (phenoxy and epoxy resins) polymers were found in Southern Ocean the surface water suggesting both long-range and local sources of origin. Whilst we found that micro and me- soplastic concentrations in seawater were significantly low (0.013 ± 0.005n/m3) compared to global averages, they were higher along the Antarctic Peninsula than the open ocean (Sub-Antarctic) stations. -
2019 Abstracts
We are delighted EDITORIAL to welcome the worldwide yeast community in Gothenburg for ICYGMB2019! The “International Yeast Conferences” started in the 1960s with a handful of delegates and since then have become THE most important event in yeast research. Now the yeast meeting to returns to Gothenburg. Many yeast researchers still remember the meeting in 2003 with over 1,100 delegates, a truly memorable event. The Life Sciences are changing, and yeast research remains at their forefront. Advancements in genome sequencing and genome editing just make yeast more exciting as model organism in basic cell biological research, genome evolution and as a tool for synthetic biology and biotechnology. One of the most important reasons for the enormous success of yeast research lies in the unique character of the international yeast research community. No other community employs such a free exchange and access to information and research tools. Nor has any other community had the ability to build – even intercontinental – consortia of critical mass to tackle large‐scale projects, such as in sequencing the first eukaryotic genome or the first comprehensive yeast knockout library. Yeast2019 is the meeting of the international yeast research community where the latest, and even unpublished results are exchanged, and new projects, alliances, and collaborations are founded. A do‐not‐miss‐event. We attempt to incorporate the present excitement in yeast research in the programme of yeast2019. We are confident that this conference will contain important news and information for all yeast researchers. Taken together, yeast2019 will provide an up‐ to‐date overview in yeast research and it will set the scene for years to come. -
Final Project Report
FINAL PROJECT REPORT Direct and indirect ecotoxicological impacts of microplastics on marine organisms Project acronym: PLASTOX Period covered: 01.01.2016 – 30.06.2019 Date of submission: 03.07.2019 Picture: Marco Capolupo (University of Bologna) Coordinating institute: SINTEF Ocean Project coordinator: Dr Andy Booth, Senior Researcher, Environmental Technology Dept., SINTEF Ocean Tel: +47 93089510 E-mail : [email protected] PLASTOX website address: https://www.sintef.no/projectweb/plastox/ CONSORTIUM Names Organisation Country SINTEF Ocean, Environmental Technology Department, Dr Andy Booth Norway Trondheim Dr Kaori Sakaguchi-Söder Technische Universität Darmstadt (TUDA), Darmstadt Germany Prof. Paula Sobral NOVA.ID FCT (NOVA-FCT), Caparica Portugal Prof. Laura Airoldi, Prof. Elena Alma Mater Studiorum - University of Bologna (UNIBO) Italy Fabbri, Prof. Giulio Zanaroli Aix-Marseille Université-Mediterranean Institute of Dr Richard Sempéré France Oceanography (AMU-MIO), Marseille Wageningen Marine Research (Wageningen University and Dr Jan Andries van Franeker Netherlands Research) (WUR), Den Helder Dr Tom Doyle, Dr Liam National University of Ireland Galway (NUIG), Galway Ireland Morrison Norwegian University of Science and Technology (NTNU) Dr Iurgi Salaberria Norway Trondheim Dr Carl van Colen Ghent University (UGhent), Ghent Belgium Dr Dorte Herzke Norwegian Institute of Air Research (NILU), Tromsø Norway Dr Kerstin Magnusson IVL Swedish Environmental Research Institute (IVL), Göteborg Sweden Prof. Geir Gabrielsen The Norwegian -
Studies with PET-Hydrolyzing Enzymes
MASTERARBEIT / MASTER’S THESIS Titel der Masterarbeit / Title of the Master‘s Thesis „Investigating the properties of PET-hydrolase and PBS- depolymerase“ verfasst von / submitted by Christoph Clemens Hinterberger, BSc angestrebter akademischer Grad / in partial fulfilment of the requirements for the degree of Master of Science (MSc) Wien, 2018/ Vienna 2018 Studienkennzahl lt. Studienblatt / A 066 830 degree programme code as it appears on the student record sheet: Studienrichtung lt. Studienblatt / Masterstudium Molekulare degree programme as it appears on Mikrobiologie, Mikrobielle Ökologie und the student record sheet: Immunbiologie Betreut von / Supervisor: Univ.-Prof. Dr. Udo Bläsi Declaration I hereby declare that this thesis was composed by myself, that the work contained herein is my own except where explicitly stated otherwise in the text, and that this work has not been submitted for any other degree or processional qualification except as specified. Acknowledgements First and foremost, I have to thank Prof. Uwe T. Bornscheuer, who gave me the opportunity of working on this topic, and Dr. Dominique Böttcher, for supervising my work. My thanks also go out to everyone in Dr. Bornscheuer’s group, who helped me along the way, especially to the ever smiling Lukas. Furthermore I have to thank people in my life which made all of this possible: My parents who support me on every step on the road, my siblings, Paul, Max, Tao Su and everyone else in the hard core, Nils and everyone else from the Pack, Linda and Michelle and finally Julia. You’ve all done more than you think. Table of Contents Declaration .............................................................................................................................................. 2 Acknowledgements ................................................................................................................................ -
Enzymatic PET Degradation
GREEN AND SUSTAINABLE CHEMISTRY CHIMIA 2019, 73, No. 9 743 doi:10.2533/chimia.2019.743 Chimia 73 (2019) 743–749 © Swiss Chemical Society Enzymatic PET Degradation Athena Papadopoulou§, Katrin Hecht§, and Rebecca Buller* Abstract: Plastic, in the form of packaging material, disposables, clothing and other articles with a short lifespan, has become an indispensable part of our everyday life. The increased production and use of plastic, however, accelerates the accumulation of plastic waste and poses an increasing burden on the environment with negative effects on biodiversity and human health. PET, a common thermoplastic, is recycled in many countries via ther- mal, mechanical and chemical means. Recently, several enzymes have been identified capable of degrading this recalcitrant plastic, opening possibilities for the biological recycling of the omnipresent material. In this review, we analyze the current knowledge of enzymatic PET degradation and discuss advances in improving the involved enzymes via protein engineering. Looking forward, the use of plastic degrading enzymes may facilitate sustain- able plastic waste management and become an important tool for the realization of a circular plastic economy. Keywords: Biocatalysis · Biodegradation · Enzyme engineering · Plastic recycling · PET Dr. Athena Papadopoulou studied Biology 1. Introduction and received her BSc from the University Plastic has become an omnipresent material in our daily life of Ioannina. She completed her MSc in and, as a consequence, the plastic industry has become the seventh Chemistry with a focus on Chemical and most important industry in Europe, employing more than 1.5 mil- Biochemical Technologies. In 2013 she lion people with a turnover of 355 billion Euros in 2017.[1] Plastic moved to Biotechnology group at the production is cheap and the generated plastic items are durable University of Ioannina to pursue her PhD and versatile. -
Program Bound to Inspire
A PROGRAM BOUND TO INSPIRE Held in conjunction with Conference Program Feeling lucky? Stop by the ASBMB booth #1421 for your chance to win one of three $250 Amazon gift cards. To participate in the contest, you must update your ASBMB member profile or join the society for the first time. WWW.ASBMB.ORG Table of Contents 2 At-a-glance 10 Sunday oral program 20 Monday oral program 30 Tuesday oral program 39 Poster sessions Program at-a-glance At-a-glance Saturday April 6 Time Location Event Convention Center 8:30 AM – 4:30 PM ASBMB Graduate Student and Postdoctoral Researcher Travel W307ABC Awardee Career Development Event Convention Center 11:30 AM – 12:00 PM ASBMB Annual Meeting Orientation for Undergraduate W306AB Students Convention Center 11:30 AM – 6:00 PM ASBMB Undergraduate Poster Competition Judges' W303ABC Orientation Convention Center 1:00 PM – 4:30 PM W304 ASBMB Undergraduate Student Research Poster Competition Convention Center 1:15 PM – 2:45 PM Career Development Workshop for Grads and Postdocs: W305A Networking Skills Convention Center 1:15 PM – 2:45 PM Career Development Workshop for Grads and Postdocs: W307D Constructing Your Elevator Pitch Convention Center 1:15 PM – 2:45 PM Career Development Workshop for Grads and Postdocs: W305B Practical Tools for Navigating Your Career Path Evolution Convention Center 4:45 PM – 5:45 PM ASBMB Undergraduate Workshop: Exploring Careers Speed W306AB Networking Convention Center 7:00 PM – 8:30 PM Valencia Ballroom EB Welcome Reception with Science Outreach Poster Session ABCD GRANT WRITING WORKSHOP June 13–15 • Washington, D.C. -
Microfiber Pollution
By Jerilyn Ritzman, WSU Extension Island County Shore Stewards Program Coordinator. Spring 2020 Microfiber Pollution Figure 1: They may seem small, but the microfibers we shed from our clothing may have significant impacts on natural ecosystems. Image Credit: G.A. Wetherbee, A.K. Baldwin, and J.F. Ranville / Public Domain. USGS Report 2019-1048 (link). Introduction When you think of microplastic pollution, what typically comes to mind? A small fragment with jagged edges? Bits of styrofoam? The tiny beads commonly found in cosmetics or hand soaps? These are all common types of microplastics (fragments of plastic smaller than 5mm), but scientists are now discovering that the most abundant type of microplastic in our environment is actually microfibers from clothing and other synthetic textiles. As scientists refine their collection methods and use smaller mesh for collection, the estimated number of microfibers in the environment increases dramatically. Microfibers are being found not just in our freshwater and marine environments, but in the sedimentary record and in the atmosphere as well. While textiles in general present a massive source of pollution from production, transportation, and disposal (did you know the average person throws away 80+ pounds of textiles per year?), this newsletter will specifically address microfiber pollution in the natural environment. It will cover which fabrics are the worst culprits, how microfibers are released into the environment, the environmental impacts, and what Shore Stewards can do to help reduce the number of loose microfibers generated in our homes. The fabrics We don’t typically devote much thought to what happens to the fibers shed from our clothing. -
Elasmobranch (Sharks and Rays) Interaction with Plastic Pollution
Elasmobranch (sharks and rays) interaction with plastic pollution from global and local perspectives, via entanglement within anthropogenic debris and synthetic fibre ingestion. Submitted by Kristian Parton, to the University of Exeter as a thesis for the degree of Masters by Research in Biological Sciences, December 2019. This thesis is available for Library use on the understanding that it is copyright material and that no quotation from the thesis may be published without proper acknowledgement. I certify that all material in this thesis which is not my own work has been identified and that any material that has previously been submitted and approved for the award of a degree by this or any other University has been acknowledged. K.J Parton (Signature)………………………………………………………… 1 Abstract Plastic pollution is a known threat to a host of marine organisms across the world. Research in recent years has exposed numerous negative impacts on some of the world’s most threatened marine species, including turtles, cetaceans and pinnipeds. The impact of plastic pollution on elasmobranchs, however, has been relatively understudied. Sharks and rays are widely accepted to be two of the most threatened marine species in the oceans, most notably due to anthropogenic impacts including direct fisheries and bycatch. Their relationship with plastic pollution is only now being investigated in further detail. Previous studies have alluded to damaging effects on sharks and rays as a result of plastic pollution but have lacked in wide synthesis of existing information and empirical evidence. In this thesis, the impact of entanglement within and ingestion of plastic is highlighted for sharks and rays both globally and locally in the North-East Atlantic. -
Crystallography in the News
Crystallography Newsletter Volume 11, No. 04, April 2019 Crystallography in the News In this issue: April 2, 2019. Researchers led by Kelly Manthei, a Postdoctoral Fellow at the University of Michigan Life Sciences Institute, found the structure of activated lecithin:cholesterol Crystallography in the News acyltransferase (LCAT) enzyme and how it helps to return excess cholesterol to the liver. Visit with Us Using X-ray crystallography, the scientists determined the mechanism of the enzyme and its reaction in association with a drug-like chemical. Join ROD on LinkedIn Product Spotlights April 3, 2019. New work from the D. E. Shaw research team covers fragment binding, HSA SAXS Standard Kit and even if you don't do fragment-based drug discovery, it's worth a read. That's because CSHL X-Ray Methods in Structural Biology the mechanisms by which fragments bind to proteins are most likely the fundamental ones by which larger molecules bind as well; this is the reductionist look at small Rigaku X-ray Forum molecule-protein interactions. Lab in the Spotlight Survey of the Month April 8, 2019. As a gifted young graduate student in physics at Cambridge in the late 1940s, June Broomhead mapped out the crystal structures of two components critical to Last Month's Survey DNA — adenine and guanine — using X-ray crystallography and painstaking calculations. Useful Link In an era of rapid scientific discovery, that research for her PhD thesis was significant. Video of the Month Recent Crystallographic Papers April 10, 2019. Based on true events, Anna Ziegler's one-act drama, Photograph 51, is a riveting and amazing story told in superior style in the curent production impeccably Book Review directed by Ellie Schwetye at West End Players Guild. -
Environmental and Biological Consequences of Microplastic Within Marine Habitats
ENVIRONMENTAL AND BIOLOGICAL CONSEQUENCES OF MICROPLASTIC WITHIN MARINE HABITATS by MARK ANTHONY BROWNE A thesis submitted to the University of Plymouth in partial fulfilment for the degree of DOCTOR OF PHILOSOPHY School of Biological Sciences October 2007 Frontispiece. Plastic debris on a beach in Looe, Cornwall, UK. Copyright Statement This copy of the thesis has been supplied on condition that anyone who consults it is understood to recognise that its copyright rests with its author and that no quotation from the thesis and no information derived fr om it may be published without the author's prior consent. f:D . Item No loo Sl0 8ýka b ý..ý ý'rZwrk "(klýSlS363,73441301" Mark Anthony Browne October 2007 ii Environmental and biological consequences of microscopic plastic within marine habitats Mark Anthony Browne Abstract Large pieces of plastic greater than a millimetre in diameter contaminate marine habitats worldwide and the associated environmental problems are well documented. In addition tiny fragments of plastic debris less than a millimetre in size have recently been reported. This thesis examines the distribution and environmental consequences of microscopic particles of plastic within marine habitats. To quantify the relative influence of wind and depositional environment on the accumulation of plastic debris, a mensurative experiment was conducted in a macrotidal Estuary. The overall trend was that material accumulated in down-wind sites. However, the relative importance of wind as a transport agent depended on the size and density of the plastic. Natural sediments are transported according to their size; but the extent to which models of sediment dynamics could be applied to the transport of plastic debris remains untested. -
Transgenerational Effects on Development Following Microplastic Exposure in Drosophila Melanogaster
Transgenerational effects on development following microplastic exposure in Drosophila melanogaster Eva Jimenez-Guri1,2, Katherine E. Roberts1, Francisca C. García1, Maximiliano Tourmente3,4, Ben Longdon1 and Brendan J. Godley1 1 Centre for Ecology and Conservation, College of Life and Environmental Sciences, University of Exeter Cornwall Campus, University of Exeter, Penryn, Cornwall, United Kingdom 2 Biology and Evolution of Marine Organisms (BEOM), Stazione Zoologica Anton Dhorn, Naples, Italy 3 Institute for Biological and Technological Research (IIByT), National Scientific and Technical Research Council (CONICET), Córdoba, Argentina 4 Centre for Cell and Molecular Biology. Faculty of Exact, Physical, and Natural Sciences, University of Córdoba, Córdoba, Argentina ABSTRACT Background. Plastic pollution affects all ecosystems, and detrimental effects to animals have been reported in a growing number of studies. However, there is a paucity of evidence for effects on terrestrial animals in comparison to those in the marine realm. Methods. We used the fly Drosophila melanogaster to study the effects that exposure to plastics may have on life history traits and immune response. We reared flies in four conditions: In media containing 1% virgin polyethylene, with no chemical additives; in media supplemented with 1% or 4% polyvinyl chloride, known to have a high content of added chemicals; and control flies in non-supplemented media. Plastic particle size ranged from 23–500 mm. We studied fly survival to viral infection, the length of the larval and pupal stage, sex ratios, fertility and the size of the resultant adult flies. We then performed crossings of F1 flies in non-supplemented media and looked at the life history traits of the F2.