Systematic Botany (2004), 29(1): pp. 147–164 q Copyright 2004 by the American Society of Plant Taxonomists Paraphyly in Tribe Onagreae: Insights into Phylogenetic Relationships of Onagraceae Based on Nuclear and Chloroplast Sequence Data RACHEL A. LEVIN,1,7 WARREN L. WAGNER,1 PETER C. HOCH,2 WILLIAM J. HAHN,3 AARON RODRIGUEZ,4 DAVID A. BAUM,5 LILIANA KATINAS,6 ELIZABETH A. ZIMMER,1 and KENNETH J. SYTSMA5 1Department of Systematic Biology, Botany, MRC 166, Smithsonian Institution, P. O. Box 37012, Washington, District of Columbia 20013-7012; 2Missouri Botanical Garden, P. O. Box 299, St. Louis, Missouri 63166-0299; 3108 White-Gravenor, Box 571003, Georgetown University, Washington, District of Columbia, 20057-1003; 4Departamento de Botan´‡ca y Zoolog´‡a, Apartado Postal 139, 45101 Zapopan, Jalisco, Mexico; 5Department of Botany, University of Wisconsin, 430 Lincoln Drive, Madison, Wisconsin 53706; 6Departamento Cienti!co de Plantas Vasculares, Museo de Ciencias Naturales, Paseo del Bosque s/n, 1900 La Plata, Provincia de Buenos Aires, Argentina 7Author for correspondence (
[email protected]) Communicating Editor: Thomas G. Lammers ABSTRACT. Onagraceae are a family of 17 genera in seven tribes, with the majority of species in tribes Onagreae and Epilobieae. Despite the species-richness of these two tribes, to date no phylogenetic study has been done with suf!cient taxon sampling to examine relationships between and within these tribes. In this study, we used DNA sequence data from one nuclear region (ITS) and two chloroplast regions (trnL-trnF and rps16) to infer phylogenetic relationships among 93 taxa across the family, with concentrated sampling in the large tribe Onagreae.