ISSN 1479-2362 Chinese Journal of Agricultural Biotechnology Volume 5 Number 3 December 2008 Chinese Journal of Agricultural Biotechnology Volume 5 Number 3 December 2008

Research Papers Genetic structure analysis of domestic duck populations in eastern China Li Hui-Fang, Song Wei-Tao, Zhu Wen-Qi, Xu Wen-Juan and Tang Qing-Ping 183 Bovine interferon-tau expression in Escherichia coli and identification of its biological activities Gao Fang-Fang, Wu Zhong-Yi and Zeng Shen-Ming 189 Inter-family distant hybridization of Oryza sativa and Oenothera biennis Chu Xiu-Cheng, Zhao Ji-Hong, Chai Yang, Zhao Jian-Feng, Zhao Yun-Yang, Jiang Li-Yan, Zhao Fen-Shan, Yu Hui-Qin, Zhao Wei-Dong and You Zhi-Qiang 197 Cloning, expression and bioactivity of chicken receptor activator of NF-κB ligand (chRANKL) Wang Yan and Hou Jia-Fa 205 Volume 5 Number 3 December 2008 Comparison of conventional plasmid vector and Semliki forest virus-derived vectors in expressing growth hormone releasing hormone (GHRH) Ren Xiao-Hui, Luo Hu-Ying, Liu Song-Cai, Zhang Ming-Jun, Ouyang Song-Ying, Li Hong-Yi and Zhang Yong-Liang 211 Activation tagging library construction and mutant phenotype analysis Cao Dong-Mei, Fan Xi-Ying, Wang Yun-Shan and Kang Li-Fang 217 SSR markers for identification of purity of melon hybrids Li Ju-Fen, Ma Guo-Bin and Xu Ling 223 Cryopreservation of boar semen using straws Gao Jun-Feng, Zheng Xiao-Feng, Ge Li-Jun, Lu Qing and Rui Rong 231 Isolation and culture of bone marrow mesenchymal stem cells from human fetus and their biological properties Zhang Yi-Hua, Dou Zhong-Ying, Shen Wen-Zheng, Yang Chun-Rong and Gao Zhi-Min 237 Identification of the cry gene in Bacillus thuringiensis strain WZ-9 and its toxicity against Henosepilachna vigintioctomaculata Song Ping, Wang Qin-Ying, Wu Hui-Xian, Lu Xiu-Jun and Wang Yong 245 Polymorphism of MC4R Asp298Asn site and its relationship with backfat thickness in commercial pigs Yang Xiao-Hui, Liu Yuan, Tang Hui, Zhang Ning-Bo, Wu Ying, Wei Shu-Dong and Jiang Yun-Liang 251 Induction of lamb follicular development and embryo production in vitro Chen Xiao-Yong, Tian Shu-Jun, Sang Run-Zi, Sun Shu-Chun, Zhao Zhu-Jun, Li Jun-Jie and Dun Wei-Tao 257 In vitro differentiation of chicken spermatogonial stem cells into adipocytes Yu Fei, Ge Jian-Hui, Ni Li-Gang, He Xian-Hong, Xu Qi and Li Bi-Chun 263 Diversity analysis of endophytic bacteria within Azolla microphylla using PCR-DGGE and electron microscopy Zheng Si-Ping, Chen Bin, Guan Xiong and Zheng Wei-Wen 269

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Aims and Scope Chinese Journal of Agricultural Biotechnology brings cutting-edge Chinese research to an international audience. This Journal takes carefully selected papers published in Chinese in the Journal of Agricultural Biotechnology, translates them and makes them available to the international scientific community. Brought to you through a unique partnership between Cambridge University Press, the China Agricultural University and the Chinese Society of Agricultural Biotechnology, the Chinese Journal of Agricultural Biotechnology will feature high-quality Chinese research translated into English presented SEED alongside specially commissioned material, providing a wider context. Chinese Journal of Agricultural Biotechnology will cover subjects such as biotechnology (cellular, chro mosomal, enzymatic, genetic and fermentation engineering) of plants, animals and relevant SCIENCE micro organisms (including nitrogen fixing bacteria and pathogens), and related biochemistry and structural analysis. RESEARCH

Editor-in-Chief Published on behalf of the International Society of Professor R Hull Seed Science John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH

Seed Science Research Editor Advisory Board is available online at Marc Alan Cohn, Louisiana State University, USA Professor Chen Zhangliang, China Agricultural Dr Rebecca Nelson, Cornell University, USA journals.cambridge.org/ssr University, Beijing, China Dr Sun Qixin, China Agricultural University, Beijing, Professor James Dale, Queensland University of China To subscribe contact Technology, Australia Dr Mark Tepfer, INRA-Versailles, France Customer Services Seed Science Research, the official journal of the Professor Fan Yunliu, CAAS, Beijing, China Professor Wu Changxin, China Agricultural in Cambridge: International Society for Seed Science, is a leading Professor Fang Rongxiang, Chinese Academy of University, Beijing, China Phone +44 (0)1223 326070 international journal featuring high-quality original papers Fax +44 (0)1223 325150 and review articles on the fundamental aspects of seed Sciences, Beijing, China Professor Xu Zhihong, Peking University Email [email protected] science, reviewed by internationally distinguished editors. Professor Huang DaFang, CAAS, Beijing, China (President), and Chinese Academy of Science in New York: The emphasis is on the physiology, biochemistry, molecular Phone (845) 353 7500 Professor Li Debao, Zhejiang University, (Vice-President) biology and ecology of seeds. Hangzhou, China Dr Zhang Qifa, Huazhong Agricultural University, Fax (845) 353 4141 Email Dr Li Jiayang, Chinese Academy of Sciences, Wuhan, China [email protected] Beijing, China Zhang Yaoping, Senior Scientist, College of Dr Li Jilun, China Agricultural University, Beijing, Agricultural and Life Sciences, Madison, USA Price information is available at China journals.cambridge.org/ssr Dr Liu Dajun, Nanjing Agricultural University, China Free email alerts Keep up-to-date with new material – Editorial Board sign up at journals.cambridge.org/register Professor Klaus Ammann, University of Bern, Dr Li Ning, China Agricultural University, Beijing, Switzerland China Dr David Burt, Roslin Institute, UK Dr Paul Teng, World Fish Center, Malaysia Dr Li Dawei, China Agricultural University, Beijing, Dr Yu Jialin, China Agricultural University, Beijing, China China

For a free online sample visit journals.cambridge.org/ssr

© China Agricultural University and Cambridge University Press 2008. All rights reserved. Published by Cambridge University Press.

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Publishing, Production, Marketing and Print only: £472 (US$866 in USA, Canada and Mexico) Subscription Sales Office: Prices include delivery by air where appropriate. Cambridge University Press The Edinburgh Building Claims for missing issues should be made immedi- Shaftesbury Road ately on receipt of the subsequent issue. Cambridge CB2 8RU UK US POSTMASTERS: Please send address corrections to Chinese Journal of Agricultural Biotechnology, For Customers in North America: Cambridge University Press, 100 Brook Hill Drive, Cambridge University Press West Nyack, New York 10994-2133, USA. Journals Fulfillment Dept 100 Brook Hill Drive Copying: This journal is registered with the West Nyack Copyright Clearance Center, 222 Rosewood Drive, New York 10994-2133 Danvers, MA 01923, USA. Organisations in the USA USA who are registered with the CCC may therefore copy Publisher: Katy Christomanou material (beyond the limits permitted by sections 107 and 108 of USA copyright law) subject to payment Chinese Journal of Agricultural Biotechnology to the CCC of the per copy fee of $16.00. This publishes carefully selected peer-reviewed papers consent does not extend to multiple copying for originally published in Chinese in the Journal of promotional or commercial purposes. Code 1479- Agricultural Biotechnology. Chinese Journal of Agri- 2362/2008/$16.00. Organisations authorised by the cultural Biotechnology is published three times a Copyright Licensing Agency may also copy material year, in print and online, by Cambridge University subject to the usual conditions. For all other use, Press in April, August and December. permission must be sought from Cambridge or the Special sales and supplements: American Branch of Cambridge University Press. The Journal accepts advertising and inserts. Any supplements of this Journal published in the course Disclaimer: The information contained herein, of the annual volume are normally supplied to sub- including any expression of opinion and any pro- scribers at no extra charge. We also provide bulk jection or forecast, has been obtained from or is reprints of suitable papers to meet teaching or pro- based upon sources believed by us to be reliable, motional requirements. The Journal also publishes but is not guaranteed as to accuracy or complete- abstracts and proceedings on behalf of academic and ness. The information is supplied without obligation corporate sponsors. and on the understanding that any person who acts upon it or otherwise changes his/her position Subscription Rates (2008) excluding VAT in reliance thereon does so entirely at his/her own Institutional subscription risk. Print and electronic access: £506 (US$930 in USA, Canada and Mexico) Cambridge University Press does not accept respon- Electronic only: £402 (US$740 in USA, Canada and sibility for any trade advertisement included in this Mexico) publication.

Downloaded from https://www.cambridge.org/core. IP address: 170.106.35.76, on 02 Oct 2021 at 13:20:10, subject to the Cambridge Core terms of use, available at https://www.cambridge.org/core/terms. https://doi.org/10.1017/S1479236209002964 Chinese Journal of Agricultural Biotechnology Volume 5 Number 3 December 2008

Research Papers Genetic structure analysis of domestic duck populations in eastern China Li Hui-Fang, Song Wei-Tao, Zhu Wen-Qi, Xu Wen-Juan and Tang Qing-Ping 183

Bovine interferon-tau expression in Escherichia coli and identification of its biological activities Gao Fang-Fang, Wu Zhong-Yi and Zeng Shen-Ming 189 Inter-family distant hybridization of Oryza sativa and Oenothera biennis Chu Xiu-Cheng, Zhao Ji-Hong, Chai Yang, Zhao Jian-Feng, Zhao Yun-Yang, Jiang Li-Yan, Zhao Fen-Shan, Yu Hui-Qin, Zhao Wei-Dong and You Zhi-Qiang 197 Cloning, expression and bioactivity of chicken receptor activator of NF-kB ligand (chRANKL) Wang Yan and Hou Jia-Fa 205

Comparison of conventional plasmid vector and Semliki forest virus-derived vectors in expressing growth hormone releasing hormone (GHRH) Ren Xiao-Hui, Luo Hu-Ying, Liu Song-Cai, Zhang Ming-Jun, Ouyang Song-Ying, Li Hong-Yi and Zhang Yong-Liang 211

Activation tagging library construction and mutant phenotype analysis Cao Dong-Mei, Fan Xi-Ying, Wang Yun-Shan and Kang Li-Fang 217 SSR markers for identification of purity of melon hybrids Li Ju-Fen, Ma Guo-Bin and Xu Ling 223 Cryopreservation of boar semen using straws Gao Jun-Feng, Zheng Xiao-Feng, Ge Li-Jun, Lu Qing and Rui Rong 231

Isolation and culture of bone marrow mesenchymal stem cells from human fetus and their biological properties Zhang Yi-Hua, Dou Zhong-Ying, Shen Wen-Zheng, Yang Chun-Rong and Gao Zhi-Min 237

Identification of the cry gene in Bacillus thuringiensis strain WZ-9 and its toxicity against Henosepilachna vigintioctomaculata Song Ping, Wang Qin-Ying, Wu Hui-Xian, Lu Xiu-Jun and Wang Yong 245 Polymorphism of MC4R Asp298Asn site and its relationship with backfat thickness in commercial pigs Yang Xiao-Hui, Liu Yuan, Tang Hui, Zhang Ning-Bo, Wu Ying, Wei Shu-Dong and Jiang Yun-Liang 251 Induction of lamb follicular development and embryo production in vitro Chen Xiao-Yong, Tian Shu-Jun, Sang Run-Zi, Sun Shu-Chun, Zhao Zhu-Jun, Li Jun-Jie and Dun Wei-Tao 257 In vitro differentiation of chicken spermatogonial stem cells into adipocytes Yu Fei, Ge Jian-Hui, Ni Li-Gang, He Xian-Hong, Xu Qi and Li Bi-Chun 263

Diversity analysis of endophytic bacteria within Azolla microphylla using PCR-DGGE and electron microscopy Zheng Si-Ping, Chen Bin, Guan Xiong and Zheng Wei-Wen 269

g China Agricultural University and Cambridge University Press, 2008

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