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176 | A001 A002 A003 A004 2017 International Meeting of the Microbiological Society of Korea A001 A003 Adhaerimonas asanensis gen. nov., sp. nov., a Novel Hydrogenophaga soli sp. nov., a Novel Species Isolated from Bacterium Isolated from a Tidal Flat in the Yellow Sea Rice Field Soil in South Korea Sophea Pheng1,2, A-young Park1, Yong-Jae Lee1, Dahye Yang and Taegun Seo* 1 1,2 Kang Hyun Lee , and Song-Gun Kim * Dongguk University Biomedi Campus 32 1Korea Research Institute of Bioscience and Biotechnology, 2University of Science and Technology A novel Gram-negative bacterial strain, designated strain S10T, was isolated from rice field soil collected in Goyang, South Korea. Cells of strain A novel strain designated YelD216T was isolated from the tidal flat of Asan S10T were strictly aerobic, motile and rod-shaped. Colonies were round, Bay in the Yellow Sea, South Korea. Strain YelD216T was facultative- convex, smooth and white. Strain S10T grew optimally at 37°C, at pH 7.0 anaerobic, Gram-negative, motile with a single polar flagellum, non-spore and at 0% (w/v) NaCl. Phylogenetic analysis of the 16S rRNA gene forming, sticky on an agar plate, short-rod-shaped, and required NaCl for sequence of strain S10T revealed that this bacterium was related to the growth. The optimal growth occurred at 25°C to 35°C, pH 7-8 and in the family Comamonadaceae, and it is related to members of the genus presence of 2% NaCl. The comparative phylogenetic analysis based on the 16S Hydrogenophaga, with Hydrogenophaga caeni KCTC 12613T being its closest rRNA showed that the strain YelD216T belongs to the family Alteromonadacea. relative (97.9% sequence similarity). The DNA G+C content of strain S10T was Higher similarities of 16S rRNA gene sequences were found with 68.17 ± 0.03 mol%. Strain S10T contained a phosphatidylethanolamine, a Bowmanella denitrificans (93.2%), Aestuariibacter salexigens (93.1%), diphosphatidylglycerol, and two unidentified aminophospholipids as the Salimonas chungwhenis (92.8%), and Alteromonas macleodii (92.7%). major polar lipids. The major fatty acids were C16:0 and summed feature 3 T Strain YelD216 contained Q-8 as a major isoprenoid quinone; polar lipid (C16:1 ω7c and/or iso-C15:0 2-OH). The predominant respiratory quinone consisted of phosphatidylethanolamine, phosphatidylglycerol, and an was ubiquinone Q-8. DNA-DNA hybridization values of strain S10T with T unidentified amino lipid. The major fatty acids were C16:0, C17:0 10-methyl, Hydrogenophaga caeni KCTC 12613 , Hydrogenophaga atypica DSM T T summed feature3 (C16:1ω6c and /or C16:1ω7c), and summed feature8 (C18:1 15342 , and Hydrogenophaga defluvii DSM 15341 were 16.1 ± 4.8%, 49.0 ω 6c and/or C18:1ω7c). The DNA G+C content was 51.3 mol%. ± 3.2%, and 21.9 ± 8.8%, respectively. Based on phylogenetic distinctiveness, Based on the different polyphasic taxonomic and low 16S rRNA gene DNA-DNA hybridization, and specific physiological and biochemical tests, sequence similarities, we propose the strain forms a novel genus and strain S10T (=KCTC 52520T =JCM 31711T) represents a novel species of the species for which the name Adhaerimonas asanensis gen. nov., sp. nov. is genus Hydrogenophaga, for which the name Hydrogenophaga soli sp. proposed. The type strain is YelD216T (= KCTC 32984T = CGMCC 1.15039T). nov. is proposed. A002 A004 Paenibacillus mobilis sp. nov., a Gram-negative Motile Flavobacterium communis sp. nov., Isolated from Bacterium Isolated from Soil Freshwater Dahye Yang and Taegun Seo* Adaeze Precious Ekwe and Seung Bum Kim* Dongguk University Biomedi Campus 32 Department of Microbiology and Molecular Biology, Chungnam National University A novel Gram-negative bacterium, designated strain S8T, was isolated A Gram-negative, yellow-pigmented, facultative-anaerobic, rod-shaped, from a soil sample from Gyeonggi Province, South Korea. Cells of strain S8T non-spore forming bacterium designated PK15T was isolated from were endospore-forming, motile by means of peritrichous flagella, and freshwater. Growth was observed at 4–40oC (optimum, 30oC), pH 6–9 rod-shaped. S8T colonies were round, convex, wavy and white. Strain S8T (optimum, 8), and in the presence of 0–0.8% NaCl (optimum, 0.4%). Strain grew optimally at 37°C, pH 7–8, and 0–2.0% NaCl (w/v). On basis of 16S PK15T exhibited both catalase and oxidase activities and was able to reduce rRNA gene sequence similarity, strain S8T were affiliated with genus nitrate. On the basis of 16S rRNA gene sequence similarity, strain PK15T Paenibacillus in the family Paenibacillaceae, Paenibacillus yonginensis was shown to belong to the genus Flavobacterium with close similarities KCTC 33428T being its closest relative (98.5% sequence similarity). The to Flavobacterium palustre S44T (97.9%) and Flavobacterium seoulense DNA G+C content of the novel strain was 53.1±0.3 mol%. Strain S8T EM1321T (97.7%). Menaquinone-6 (MK-6) was the major respiratory quinone contained four phospholipids, four aminophospholipids, an aminolipid while the G + C content of the genomic DNA was 35.5 (±0.9) mol%. The and three unidentified lipids. The major fatty acid was found to be major polar lipids were phosphatidylethanolamine, three unknown anteiso-branched C15:0. The predominant quinone was MK-7 menaquinone. aminolipids, one unknown aminophospholipids and three unknown polar T The DNA-DNA hybridization value of strain S8 with Paenibacillus yonginensis lipids. The predominant fatty acids were anteiso-C15:0 (17.3%), a summed T T KCTC 33428 and Paenibacillus physcomitrellae DSM 29851 were 44.10% feature comprising C16:1 ω7c and/or C16:1 ω6c (15.1%) and iso-C15:0 (10.0%). and 32.02%. Based on DNA-DNA hybridization, biochemical analysis, Chemotaxonomic data supported the affiliation of strain PK15T to the phylogenetic and physiological tests, strain S8T represents a novel species genus Flavobacterium. The results of the physiological and biochemical of the genus Paenibacillus, for which the name Paenibacillus mobilis sp. tests allowed genotypic and phenotypic differentiation of strain PK15T nov. is proposed. from closely related species. It is therefore evident that PK15T represents a new species, for which the name Flavobacterium communis sp. nov. is proposed with the type strain PK15T (=KCTC 52562T). 176 | www.msk.or.kr Poster A005 A007 Deinococcus gammatolerans sp. nov., a Novel Bacterium Xanthomonas arboricola pv. juglandis Isolated from Walnut Isolated from Soil, South Korea Tree in Korea Sathiyaraj Srinivasan1, Myung-Suk Kang2, and Myung Kyum Kim1* Hyun-sun Kim and Sang-tae Seo* 1Department of Bio & Environmental Technology, College of Natural Science, National Institute of Forest Science Seoul Women’s University, 2Microorganism Resources Division, National Institute of Biological Resources Xanthomonas arboricola is a bacteria, Gram-staining-negative and rod- shaped. The bacteria can cause diseases in many plants and is divided into A Gram-positive, catalase and oxidase positive short-rod-shaped bacterial various pathovars according to the host. One of pathovars, Xanthomonas strain designated as Ant2-2T was isolated from soil in South Korea. Cells arboricola pv. juglandis cause walnut blight in walnut tree and reduces showed high gamma-ray and UVC radiation resistance. The 16S rRNA yields. The bacteria can infect many organs of walnut including catkins, sequence of strain Ant2-2T represents a novel subline within the genus green branches, leaves and nuts and infected sites changed to black. The Deinococcus in the family Deinococcaceae. The 16S rRNA gene sequences disease was detected in all major walnut growing areas. But, the disease of the strain Ant2-2T were indistinguishable and showed 97.5~91.3% was not detected in Korea. similarity levels with other Deinococcus species. The strain showed the In 2016, we found walnut trees turned to black in Korea. The symptoms of typical chemotaxonomic characteristics of the genus Deinococcus, with trees were very similar to walnut blight. We isolated various bacteria from the presence of respiratory quinone as menaquinone 8; the major fatty walnut trees and collected the colonies, mucoid and yellow morphology in acids are summed feature 3 (composed of C16:1 ω7c / C16:1 ω6c), C15:1ω6c, NA (Nutrient Agar) medium. PCR was performed for 16S rRNA gene. As a T and C16:0. The DNA G+C content of the strain Ant2-2 is 57.3 mol%. The result of BLAST, these colonies showed various bacteria including Pantoea, polar lipids profile included major amounts of phosphatidylglycerol, Pseudomonas, and Xanthomonas. Most of them were Xanthomonas phosphatidylcholine, and unknown aminolipid. On the basis of its arboricola pv. juglandis strain Xaj 417(98.0% 16S rRNA gene sequence phenotypic and genotypic properties, and phylogenetic distinctiveness similarity). Phylogenetic analysis, based on 16S rRNA gene sequencing, strain Ant2-2T should be classified in a novel species in the genus showed that the bacteria was most closely related to Xanthomonas Deinococcus, for which the name Deinococcus gammatolerans sp. nov. is arboricola pv. juglandis NCPPB411T and LMG747T(100%). proposed. [Supported by grants from National Institute of Forest Science] [This work was supported by a research grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR201601113).] A006 A008 Deinococcus koreense sp. nov., a Novel Bacterium of Isolated Genome Comparison of 12 Lactobacillus Species for Species- from a Soil specific Media Myung Kyum Kim1, Myung-Suk Kang2, and Sathiyaraj Srinivasan1* Inhwan
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