Actinospica Durhamensis Sp. Nov., Isolated from a Spruce Forest Soil
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http://wjst.wu.ac.th Natural Sciences Diversity Analysis of an Extremely Acidic Soil in a Layer of Coal Mine Detected the Occurrence of Rare Actinobacteria Megga Ratnasari PIKOLI1,*, Irawan SUGORO2 and Suharti3 1Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Syarif Hidayatullah Jakarta, Ciputat, Tangerang Selatan, Indonesia 2Center for Application of Technology of Isotope and Radiation, Badan Tenaga Nuklir Nasional, Jakarta Selatan, Indonesia 3Department of Chemistry, Faculty of Science and Computation, Universitas Pertamina, Simprug, Jakarta Selatan, Indonesia (*Corresponding author’s e-mail: [email protected], [email protected]) Received: 7 September 2017, Revised: 11 September 2018, Accepted: 29 October 2018 Abstract Studies that explore the diversity of microorganisms in unusual (extreme) environments have become more common. Our research aims to predict the diversity of bacteria that inhabit an extreme environment, a coal mine’s soil with pH of 2.93. Soil samples were collected from the soil at a depth of 12 meters from the surface, which is a clay layer adjacent to a coal seam in Tanjung Enim, South Sumatera, Indonesia. A culture-independent method, the polymerase chain reaction based denaturing gradient gel electrophoresis, was used to amplify the 16S rRNA gene to detect the viable-but-unculturable bacteria. Results showed that some OTUs that have never been found in the coal environment and which have phylogenetic relationships to the rare actinobacteria Actinomadura, Actinoallomurus, Actinospica, Streptacidiphilus, Aciditerrimonas, and Ferrimicrobium. Accordingly, the highly acidic soil in the coal mine is a source of rare actinobacteria that can be explored further to obtain bioactive compounds for the benefit of biotechnology. -
Corynebacterium Sp.|NML98-0116
1 Limnochorda_pilosa~GCF_001544015.1@NZ_AP014924=Bacteria-Firmicutes-Limnochordia-Limnochordales-Limnochordaceae-Limnochorda-Limnochorda_pilosa 0,9635 Ammonifex_degensii|KC4~GCF_000024605.1@NC_013385=Bacteria-Firmicutes-Clostridia-Thermoanaerobacterales-Thermoanaerobacteraceae-Ammonifex-Ammonifex_degensii 0,985 Symbiobacterium_thermophilum|IAM14863~GCF_000009905.1@NC_006177=Bacteria-Firmicutes-Clostridia-Clostridiales-Symbiobacteriaceae-Symbiobacterium-Symbiobacterium_thermophilum Varibaculum_timonense~GCF_900169515.1@NZ_LT827020=Bacteria-Actinobacteria-Actinobacteria-Actinomycetales-Actinomycetaceae-Varibaculum-Varibaculum_timonense 1 Rubrobacter_aplysinae~GCF_001029505.1@NZ_LEKH01000003=Bacteria-Actinobacteria-Rubrobacteria-Rubrobacterales-Rubrobacteraceae-Rubrobacter-Rubrobacter_aplysinae 0,975 Rubrobacter_xylanophilus|DSM9941~GCF_000014185.1@NC_008148=Bacteria-Actinobacteria-Rubrobacteria-Rubrobacterales-Rubrobacteraceae-Rubrobacter-Rubrobacter_xylanophilus 1 Rubrobacter_radiotolerans~GCF_000661895.1@NZ_CP007514=Bacteria-Actinobacteria-Rubrobacteria-Rubrobacterales-Rubrobacteraceae-Rubrobacter-Rubrobacter_radiotolerans Actinobacteria_bacterium_rbg_16_64_13~GCA_001768675.1@MELN01000053=Bacteria-Actinobacteria-unknown_class-unknown_order-unknown_family-unknown_genus-Actinobacteria_bacterium_rbg_16_64_13 1 Actinobacteria_bacterium_13_2_20cm_68_14~GCA_001914705.1@MNDB01000040=Bacteria-Actinobacteria-unknown_class-unknown_order-unknown_family-unknown_genus-Actinobacteria_bacterium_13_2_20cm_68_14 1 0,9803 Thermoleophilum_album~GCF_900108055.1@NZ_FNWJ01000001=Bacteria-Actinobacteria-Thermoleophilia-Thermoleophilales-Thermoleophilaceae-Thermoleophilum-Thermoleophilum_album -
Table S5. the Information of the Bacteria Annotated in the Soil Community at Species Level
Table S5. The information of the bacteria annotated in the soil community at species level No. Phylum Class Order Family Genus Species The number of contigs Abundance(%) 1 Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus cereus 1749 5.145782459 2 Bacteroidetes Cytophagia Cytophagales Hymenobacteraceae Hymenobacter Hymenobacter sedentarius 1538 4.52499338 3 Gemmatimonadetes Gemmatimonadetes Gemmatimonadales Gemmatimonadaceae Gemmatirosa Gemmatirosa kalamazoonesis 1020 3.000970902 4 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas indica 797 2.344876284 5 Firmicutes Bacilli Lactobacillales Streptococcaceae Lactococcus Lactococcus piscium 542 1.594633558 6 Actinobacteria Thermoleophilia Solirubrobacterales Conexibacteraceae Conexibacter Conexibacter woesei 471 1.385742446 7 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas taxi 430 1.265115184 8 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas wittichii 388 1.141545794 9 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas sp. FARSPH 298 0.876754244 10 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sorangium cellulosum 260 0.764953367 11 Proteobacteria Deltaproteobacteria Myxococcales Polyangiaceae Sorangium Sphingomonas sp. Cra20 260 0.764953367 12 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas panacis 252 0.741416341 -
Probing the Biosynthetic Diversity of Actinobacteria 29-01-2018
BSc. A. Roeters - Probing the biosynthetic diversity of actinobacteria 29-01-2018 PROBING THE BIOSYNTHETIC DIVERSITY OF ACTINOBACTERIA MSc. Thesis by Arne Roeters, supervised by dr. MH Medema and JC Navarro Munoz PhD. Bioinformatics department Wageningen university. ABSTRACT certain plants. In these relationships the actinobacteria provide nitrogen to the plant and in The Actinobacteria are a large phylum of Gram- return they take some of the plants saccharide 1,2 positive bacteria of which we harvest many reserves . Maybe even more important and interesting about these bacteria, are their clinically useful natural products. A large portion of secondary metabolites that can be used for medical these clinically useful products are made by the purposes3. A large part of the clinically available largest genus within this phylum, called antibiotics come from Actinobacteria, and Streptomyces. These products are made by especially the largest genus Streptomyces. This biosynthetic gene clusters (BGCs), which are genus produces over two-third of the clinically physically clustered genes on the genome. To find useful natural antibiotics with its natural product 4 more of these natural compounds, genome mining biosynthetic gene clusters . Not nearly all-natural compounds have been found yet, meaning that has become one of the most important tools in there might still be many more useful compounds bioinformatics. This new technique has given rise that are made by the biosynthetic pathways of to programs like antiSMASH (Medema, et al., Actinobacteria. These biosynthetic pathways 2011). Programs like this have created new consist of genes that are physically clustered challenges due to the large amount of BGCs they together on the chromosome forming so called 5–7 mine, to narrow the search for new interesting biosynthetic gene clusters (BGCs) . -
Challenging the Anthropocentric Emphasis on Phenotypic Testing in Prokaryotic Species Descriptions: Rip It up and Start Again
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Frontiers - Publisher Connector OPINION published: 17 June 2015 doi: 10.3389/fgene.2015.00218 Challenging the anthropocentric emphasis on phenotypic testing in prokaryotic species descriptions: rip it up and start again Iain C. Sutcliffe * Applied Sciences, Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, UK Keywords: genomics, phylogeny, species, systematics, taxonomy “The benefits of specialization are tempered by the possibility that specialized groups become isolated, resist innovation, and engage in destructive competitiveness” (Specialized Science, Casadevall and Fang 2014). Prokaryotic systematics is a highly specialized field and yet has fundamental reach and significance given that it provides the framework and, importantly, the names which we use to describe most of the microbial world. Names are given to prokaryotic taxa under the jurisdiction of the International Code of Nomenclature of Prokaryotes (ICNP; Lapage et al., 1992) and the vast majority of papers in prokaryotic systematics are descriptions that name taxa, particularly novel species and genera. Edited by: Indeed, this century has seen a significant growth in the number of prokaryotic species named Radhey S. Gupta, (Tamames and Rosselló -Móra, 2012; Oren and Garrity, 2014), with ∼900 new names for Bacteria McMaster University, Canada and Archaea published (either validly or effectively) in 2014. Mostly this growth in taxonomic Reviewed by: activity is in Asia, with declines elsewhere (Tamames and Rosselló -Móra, 2012; Oren and Garrity, Ramon Rossello-Mora, 2014). Institut Mediterrani d’Estudis Despite this progress, prokaryotic systematics has become isolated from mainstream Avançats, Spain microbiology and resistant to innovation. -
Inter-Domain Horizontal Gene Transfer of Nickel-Binding Superoxide Dismutase 2 Kevin M
bioRxiv preprint doi: https://doi.org/10.1101/2021.01.12.426412; this version posted January 13, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Inter-domain Horizontal Gene Transfer of Nickel-binding Superoxide Dismutase 2 Kevin M. Sutherland1,*, Lewis M. Ward1, Chloé-Rose Colombero1, David T. Johnston1 3 4 1Department of Earth and Planetary Science, Harvard University, Cambridge, MA 02138 5 *Correspondence to KMS: [email protected] 6 7 Abstract 8 The ability of aerobic microorganisms to regulate internal and external concentrations of the 9 reactive oxygen species (ROS) superoxide directly influences the health and viability of cells. 10 Superoxide dismutases (SODs) are the primary regulatory enzymes that are used by 11 microorganisms to degrade superoxide. SOD is not one, but three separate, non-homologous 12 enzymes that perform the same function. Thus, the evolutionary history of genes encoding for 13 different SOD enzymes is one of convergent evolution, which reflects environmental selection 14 brought about by an oxygenated atmosphere, changes in metal availability, and opportunistic 15 horizontal gene transfer (HGT). In this study we examine the phylogenetic history of the protein 16 sequence encoding for the nickel-binding metalloform of the SOD enzyme (SodN). A comparison 17 of organismal and SodN protein phylogenetic trees reveals several instances of HGT, including 18 multiple inter-domain transfers of the sodN gene from the bacterial domain to the archaeal domain. -
Ce Document Est Le Fruit D'un Long Travail Approuvé Par Le Jury De Soutenance Et Mis À Disposition De L'ensemble De La Communauté Universitaire Élargie
AVERTISSEMENT Ce document est le fruit d'un long travail approuvé par le jury de soutenance et mis à disposition de l'ensemble de la communauté universitaire élargie. Il est soumis à la propriété intellectuelle de l'auteur. Ceci implique une obligation de citation et de référencement lors de l’utilisation de ce document. D'autre part, toute contrefaçon, plagiat, reproduction illicite encourt une poursuite pénale. Contact : [email protected] LIENS Code de la Propriété Intellectuelle. articles L 122. 4 Code de la Propriété Intellectuelle. articles L 335.2- L 335.10 http://www.cfcopies.com/V2/leg/leg_droi.php http://www.culture.gouv.fr/culture/infos-pratiques/droits/protection.htm Thèse Présentée et soutenue publiquement pour l’obtention du titre de DOCTEUR DE L’UNIVERSITE DE LORRAINE Mention « Biologie et écologie des forêts et des agrosystèmes » par Lauralie MANGEOT-PETER Etude des facteurs biotiques et abiotiques influant sur la structuration et la composition du microbiote racinaire du Peuplier Soutenance le 3 mars 2020 Membres du jury : Dr Feth-el-Zahar HAICHAR - Rapportrice Maître de Conférences, Université de Lyon Pr Daniel WIPF - Rapporteur Professeur, Université de Bourgogne Pr Michel CHALOT - Examinateur Professeur, Université de Lorraine Dr Stéphane HACQUARD - Examinateur Chargé de Recherche, Max Planck Institute Dr Francis MARTIN - Directeur de thèse Directeur de Recherche, INRAE Nancy Dr Aurélie DEVEAU - Co-directrice de thèse Chargée de Recherche, INRAE Nancy UMR 1136 INRAE / Université de Lorraine, Interactions Arbres – Micro-organismes INRAE Centre Grand Est - Nancy – 54280 Champenoux Faculté des Sciences et Technologies – 54500 Vandoeuvre-lès-Nancy Remerciements Remerciements Pour commencer, je souhaite remercier les membres de mon jury de thèse Zahar Haichar, Daniel Wipf, Stéphane Hacquard et Michel Chalot qui ont accepté d’évaluer mes travaux. -
Of Bergey's Manual
BERGEY’S MANUAL® OF Systematic Bacteriology Second Edition Volume Five The Actinobacteria, Part A and B BERGEY’S MANUAL® OF Systematic Bacteriology Second Edition Volume Five The Actinobacteria, Part A and B Michael Goodfellow, Peter Kämpfer, Hans-Jürgen Busse, Martha E. Trujillo, Ken-ichiro Suzuki, Wolfgang Ludwig and William B. Whitman EDITORS, VOLUME FIVE William B. Whitman DIRECTOR OF THE EDITORIAL OFFICE Aidan C. Parte MANAGING EDITOR EDITORIAL BOARD Fred A. Rainey, Chairman, Peter Kämpfer, Vice Chairman, Paul De Vos, Jongsik Chun, Martha E. Trujillo and William B. Whitman WITH CONTRIBUTIONS FROM 116 COLLEAGUES William B. Whitman Bergey’s Manual Trust Department of Microbiology 527 Biological Sciences Building University of Georgia Athens, GA 30602-2605 USA ISBN 978-0-387-95043-3 ISBN 978-0-387-68233-4 (eBook) DOI 10.1007/978-0-387-68233-4 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2012930836 © 2012, 1984–1989 Bergey’s Manual Trust Bergey’s Manual is a registered trademark of Bergey’s Manual Trust. All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. -
Biology and Biotechnology of Actinobacteria Biology and Biotechnology of Actinobacteria Joachim Wink Fatemeh Mohammadipanah Javad Hamedi Editors
Joachim Wink Fatemeh Mohammadipanah Javad Hamedi Editors Biology and Biotechnology of Actinobacteria Biology and Biotechnology of Actinobacteria Joachim Wink Fatemeh Mohammadipanah Javad Hamedi Editors Biology and Biotechnology of Actinobacteria Editors Joachim Wink Fatemeh Mohammadipanah Microbial Strain Collection; College of Science Helmholtz- Centre for Infection Research University of Tehran Braunschweig Tehran Germany Iran Javad Hamedi College of Science University of Tehran Tehran Iran ISBN 978-3-319-60338-4 ISBN 978-3-319-60339-1 (eBook) DOI 10.1007/978-3-319-60339-1 Library of Congress Control Number: 2017955832 © Springer International Publishing AG 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. -
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Diversity of Actinomycetes from Soil Samples Collected from Lombok Island, Indonesia Puspita Lisdiyanti1*, Tomohiko Tamura2, Shanti Ratnakomala1, Roni Ridwan1, Gina Kartina1, Yulin Lestari3, Ando Katsuhiko2, and Yantyati Widyastuti1 1Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Indonesia 2Department of Biotechnology, National Institute of Technology and Evaluation (NITE), Japan 3Department of Biology, Bogor Agricultural University, Indonesia Abstract A total of 137 strains of Actinomycetes were isolated from 14 soil samples collected in 3 part of Lombok Island, Indonesia using SDS-YE (SY) and Rehydration and Centrifugation (RC) isolation methods and Humic Acic Vitamin (HV) agar as isolation media. All the isolates were identified by morphological characteristic and by analysis of 16S rRNA gene sequence. On the basis of their morphology and 16S rRNA gene sequence, 67% of isolates were belonged to the Streptomyces Group and 33% of isolates were belonged to the Rare- Actinomycetes (Non-Streptomyces) Group. Nine families and 15 genera were found from the samples. It is indicated the richness of actinomycetes in these area. The genus Streptomyces is the most abundant in all soil samples, occupying 67% of all isolates by using SY method; while the genus Actinoplanes is mainly found by using RC as isolation method. Two strains belong to the genus Virgisporangium and Catenulispora that showed taxonomic interest need further study for describing as ne species. Key words: Actinomycetes, Streptomyces Group, Non-Streptomyces Group, Diversity ------------------------- *Corresponding author Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia Tel. +62-21-8754587, Fax. +62-21-8754588 E-mail. [email protected] Introduction are commercially important, either in the production of antibiotics and other bioactive Actinomycetes are a group of gram- secondary metabolites, or in useful biological positive bacteria that have high G+C contents. -
Contents Topic 1. Introduction to Microbiology. the Subject and Tasks
Contents Topic 1. Introduction to microbiology. The subject and tasks of microbiology. A short historical essay………………………………………………………………5 Topic 2. Systematics and nomenclature of microorganisms……………………. 10 Topic 3. General characteristics of prokaryotic cells. Gram’s method ………...45 Topic 4. Principles of health protection and safety rules in the microbiological laboratory. Design, equipment, and working regimen of a microbiological laboratory………………………………………………………………………….162 Topic 5. Physiology of bacteria, fungi, viruses, mycoplasmas, rickettsia……...185 TOPIC 1. INTRODUCTION TO MICROBIOLOGY. THE SUBJECT AND TASKS OF MICROBIOLOGY. A SHORT HISTORICAL ESSAY. Contents 1. Subject, tasks and achievements of modern microbiology. 2. The role of microorganisms in human life. 3. Differentiation of microbiology in the industry. 4. Communication of microbiology with other sciences. 5. Periods in the development of microbiology. 6. The contribution of domestic scientists in the development of microbiology. 7. The value of microbiology in the system of training veterinarians. 8. Methods of studying microorganisms. Microbiology is a science, which study most shallow living creatures - microorganisms. Before inventing of microscope humanity was in dark about their existence. But during the centuries people could make use of processes vital activity of microbes for its needs. They could prepare a koumiss, alcohol, wine, vinegar, bread, and other products. During many centuries the nature of fermentations remained incomprehensible. Microbiology learns morphology, physiology, genetics and microorganisms systematization, their ecology and the other life forms. Specific Classes of Microorganisms Algae Protozoa Fungi (yeasts and molds) Bacteria Rickettsiae Viruses Prions The Microorganisms are extraordinarily widely spread in nature. They literally ubiquitous forward us from birth to our death. Daily, hourly we eat up thousands and thousands of microbes together with air, water, food. -
Metagenome Sequence of Elaphomyces Granulatus From
bs_bs_banner Environmental Microbiology (2015) 17(8), 2952–2968 doi:10.1111/1462-2920.12840 Metagenome sequence of Elaphomyces granulatus from sporocarp tissue reveals Ascomycota ectomycorrhizal fingerprints of genome expansion and a Proteobacteria-rich microbiome C. Alisha Quandt,1*† Annegret Kohler,2 the sequencing of sporocarp tissue, this study has Cedar N. Hesse,3 Thomas J. Sharpton,4,5 provided insights into Elaphomyces phylogenetics, Francis Martin2 and Joseph W. Spatafora1 genomics, metagenomics and the evolution of the Departments of 1Botany and Plant Pathology, ectomycorrhizal association. 4Microbiology and 5Statistics, Oregon State University, Corvallis, OR 97331, USA. Introduction 2Institut National de la Recherché Agronomique, Centre Elaphomyces Nees (Elaphomycetaceae, Eurotiales) is an de Nancy, Champenoux, France. ectomycorrhizal genus of fungi with broad host associa- 3Bioscience Division, Los Alamos National Laboratory, tions that include both angiosperms and gymnosperms Los Alamos, NM, USA. (Trappe, 1979). As the only family to include mycorrhizal taxa within class Eurotiomycetes, Elaphomycetaceae Summary represents one of the few independent origins of the mycorrhizal symbiosis in Ascomycota (Tedersoo et al., Many obligate symbiotic fungi are difficult to maintain 2010). Other ectomycorrhizal Ascomycota include several in culture, and there is a growing need for alternative genera within Pezizomycetes (e.g. Tuber, Otidea, etc.) approaches to obtaining tissue and subsequent and Cenococcum in Dothideomycetes (Tedersoo et al., genomic assemblies from such species. In this 2006; 2010). The only other genome sequence pub- study, the genome of Elaphomyces granulatus was lished from an ectomycorrhizal ascomycete is Tuber sequenced from sporocarp tissue. The genome melanosporum (Pezizales, Pezizomycetes), the black assembly remains on many contigs, but gene space perigord truffle (Martin et al., 2010).