Volume 18(1), 117- 122, 2014 JOURNAL of Horticulture, Forestry and Biotechnology www.journal-hfb.usab-tm.ro Optimization of DNA isolation from four species of Rhododendron from Europe Caprar M.1, 2, Cantor Maria2*, Sicora Oana1, Copaci Cristina 1, Sicora C.1 1)Biological Research Center, Botanical Garden “Vasile Fati” Jibou, Parcului Street, no.14, 455200 Jibou, Romania; 2)University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Faculty of Horticulture, Mănăștur Street, no 3-5,4000472 Cluj-Napoca, Romania; *Corresponding author. Email:
[email protected] Abstract DNA isolation is a procedure used to obtain genetic material Key words from different organisms in order to use it in subsequent molecular analysis. The quantity and quality of the material are very important as the result from rhododendron, isolate DNA the next steps depend on it, respectively the widely used techniques of Kits, protocols, DNA molecular biology such as RAPD, RFLP, AFLP, sequencing and others. The concentration work shows the results obtained in isolating DNA from leaves with different extraction kits from four European species of Rhododendron, Rhododendron luteum, Rhododendron ferrugineum, Rhododendron myrtifolium and Rhododendron hirsutum. All the extraction protocols followed have three main stages: the destruction of the cellular integrity and the release of DNA in a homogenate called cell lysate, the purification of DNA from RNA, protein and other metabolic products, obtaining DNA in the desired concentration and known purity. The experiment used 3 DNA extraction kits aiming at both quantity and quality of the DNA, as well as the cost and complexity related to each kit. Rhododendrons, including azaleas, are a well- For total genomic DNA isolation several known, diverse and popular group of plants with a long methods have been used.