Received: 28 November 2018 | Revised: 13 February 2019 | Accepted: 13 February 2019 DOI: 10.1002/ece3.5029 ORIGINAL RESEARCH Genomic, ecological, and morphological approaches to investigating species limits: A case study in modern taxonomy from Tropical Eastern Pacific surgeonfishes William B. Ludt1 | Moisés A. Bernal2 | Erica Kenworthy3 | Eva Salas4 | Prosanta Chakrabarty3 1National Museum of Natural History, Smithsonian Institution, Abstract Washington, District of Columbia A wide variety of species are distinguished by slight color variations. However, mo- 2 Department of Biological Sciences, 109 lecular analyses have repeatedly demonstrated that coloration does not always cor- Cooke Hall, State University of New York at Buffalo, Buffalo, New York respond to distinct evolutionary histories between closely related groups, suggesting 3Ichthyology Section, 119 Foster Hall, that this trait is labile and can be misleading for species identification. In the present Museum of Natural Science, Department of Biological Sciences, Louisiana State study, we analyze the evolutionary history of sister species of Prionurus surgeon- University, Baton Rouge, Louisiana fishes in the Tropical Eastern Pacific (TEP), which are distinguished by the presence 4 FISHBIO, Santa Cruz, California or absence of dark spots on their body. We examined the species limits in this system Correspondence using comparative specimen‐based approaches, a mitochondrial gene (COI), more William B. Ludt, National Museum of than 800 nuclear loci (Ultraconserved Elements), and abiotic niche comparisons. The Natural History, Smithsonian Institution, Washington, DC. results indicate there is a complete overlap of meristic counts and morphometric Email:
[email protected] measurements between the two species. Further, we detected multiple individuals Funding information with intermediate spotting patterns suggesting that coloration is not diagnostic.