Biotechnology in Animal Husbandry 34 (2), p 239-249, 2018 ISSN 1450-9156 Publisher: Institute for Animal Husbandry, Belgrade-Zemun UDC 632.4:633.15 https://doi.org/10.2298/BAH1802239K TOXIGENIC FUNGAL AND MYCOTOXIN CONTAMINATION OF MAIZE SAMPLES FROM DIFFERENT DISTRICTS IN SERBIA Vesna Krnjaja1, Slavica Stanković2, Miloš Lukić1, Nenad Mićić1, Tanja Petrović3, Zorica Bijelić1, Violeta Mandić1 1Institute for Animal Husbandry, Autoput 16, 11080, Belgrade-Zemun, Serbia 2Maize Research Institute “Zemun Polje“, Slobodana Bajića 1, 11185, Belgrade-Zemun, Serbia 3Institute of Food Technology and Biochemistry, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbia Corresponding author:
[email protected] Original scientific paper Abstract: This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp.