21.10.2014SDHAF1 antibody (pAb)

Rabbit Anti-Human/Mouse complex assembly factor 1 (SDH assembly factor 1)

Instruction Manual

Catalog Number PK-AB718-6879 Synonyms SDHAF1 Antibody: Succinate dehydrogenase complex assembly factor 1, SDH assembly factor 1 Description SDHAF1 (Succinate dehydrogenase complex assembly factor 1) is one of the subunits of the succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain. SDHAF1 resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency). Unlike the related SDHAF2, SDHAF1 is not thought to be a tumor suppressor. Quantity 100 µg Source / Host Rabbit Immunogen SDHAF1 antibody was raised against an 18 synthetic peptide near the carboxy terminus of human SDHAF1. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse Specificity SDHAF1 antibody is predicted to not cross-react with other SDHAF protein family members. Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB, ICC, IF |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually. SDHAF1 antibody can be used for detection of SDHAF1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 5 µg/mL. Images Available upon request. References Ghezzi D, Goffrini P, Uziel G, et al. SDHAF1, encoding a LYR complex-II specific assembly factor, is mutated in SDH-defective infantile leukoencephalopathy. Nat. Genet. 2009; 41:654-6. Hensen EE and Bayley JP. Recent advances in the genetics of SDH-related paraganglioma and pheochromocytoma. Fam. Cancer 2011; 10:355-63.

Images Available upon request. Related Products Cat. No. PK-AB718-6879P - SDHAF1 Peptide Cat. No. PK-AB718-1282 - 3T3 (NIH) Cell Lysate

FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

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