(Iris Aurantica) in Syria
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Review Article Curr Trends Biomedical Eng & Biosci Volume 1 Issue 5 - February 2017 Copyright © All rights are reserved by Jean-Marie Exbrayat Wild Golden Iris (Iris aurantica) in Syria Khalil AL Maarri*, Thuraya Abou zedan and Nabil AL Batal Faculty of Agriculture, Damascus University, Syria Submission: February 14, 2017; Published: February 27, 2017 *Corresponding author: Khalil AL Maarri, Damascus University, Faculty of Agriculture, Damascus, Syria, Email: Abstract Iris aurantica is a rhizomatous perennial, from the south mountain of Syria (JabalAl Drouze), it has a compact rhizomein vitro and propagation falcate leaves. and conservation,It has a slender and stem cryopreservation with flowers golden of Iris yellowaurantica to coppery-brown. The golden iris in Syria is becoming rare due to destruction of their natural habit. In this paper, plant classification, botanical description, morphological, geographical, chemical composition, Keywords: are investigated. Abbreviations:Morphological; Botanical description; Micro propagation; Conservation; Chemical composition MS: Murashige and Skoog; HF-MS: Hormon Free Murashige and Skoog; PVS2: Plant Vitrification Solution 2; LN: Liquid Nitrogen Introduction urbanization, migration, determental climatic and environmental changes, adding the huge destruction of plant biodiversity by the endemic species, some of which belong to genus Lilium, Crocus, Syrian flora has 3247 species [1]. There are many of hard war since 5 years [6]. Tulips and Iris. Iris is the largest and most complicated genus morphological, geographical, chemical composition, genetic of iridaceae, which includes over 300 species [2]. This includes In this paper, Plant classification, botanical description, variability, in vitro propagation and conservation, and some of the world most popular and varied garden flowers that cryopreservation of Iris aurantica are originatedIris in bothis considered Japan and as the a Mediterraneanwild perennial [3].herbaceous were investigated. In Syria, Scientific Classification plant that subjected to strict protection, though Iris grows Iris aurantica L is one of the important species that belongs naturally in many regions of Syria. It presents some 30 species to the family Iridaceae, endemic to Jabal Al `Arab, rhizomatous Apogon, Pogonias, Xiphion, Guno and oncocyclus which includes grown in Syria [1]. There are five subgenus found in the world. most of the Syrian species, that are considered as rare endemic plants, characterized by special, beautiful forms that have a great (with thick, creeping underground stems) [7]. importance in applied studies for genetic biodiversity, such as A. Kingdom: Plantae. Asparagales Iris aurantiaca B. Unranked: Angiosperm, Monocots. Order irideae. Iris aurantica Dinsm. C. Family: Iridaceae. Subfamily: Iridoideae, Tribe: Iris. , of Syria was first discovered by Dinsmor aurantica. on the Tell Quleib in Syria. Mouterde found it in several other D. Genus: Iris, Subgenus: Oncocyclus. Species: places in Djebel Druze, Tell Qouleib, Kafer, Tell Jaffna, Mayamas, Description of Iris aurantica Sahwet-El-Khodr [1] and Distribution in the Djebel Druze at Iris aurantica is a perennial plant, growing from compact about 1600m [4]. Dr. Werkmeister, Professor of Botany at the Botanical Institute, Geisenheim am Rhein, Germany which had the opportunity to collecting the golden Iris in 1961,and cultured rhizome that reaches up to 10-14 cm long. Rhizome develops it in his garden. The golden Iris flowered in Europe in June one from axillary buds, it allows new shoots to grow upwards. The golden iris rhizomes planted underground about 5-10cm in month later than in their natural habit in Syria [5]. proteins, starches and lipids, these nutrients become useful for destruction of their natural habit, as well as over harvesting rocky soils (Figure 1). It uses rhizomes to store nutrients like Iris plants in Syria are becoming rare due to both ongoing the plant when new shoots will be formed in early spring. of wild species and the influencing of modernization, i.e., Curr Trends Biomedical Eng & Biosci 1(5): CTBEB.MS.ID.555573 (2017) 00111 Current Trends in Biomedical Engineering & Biosciences golden yellow to coppery-brown, 8-9cm long, 5-5.5 wide, with 4cm wide, abovate, with minute purplish red spots and very fine purple veins. The falls are oblong-shaped, 7cm long, and fine reddish veins. It has golden yellow with fine purple. Style branches (stigma) keeled, and have lobes tips that are a similar color like falls (Figure 3). After the iris has flowered, it produces a seed capsule, 8cm long, rather narrow. The golden iris flowered in Europe in June, a whole month later than in their habitat in Syria. The unexpectedly cold European weather in the spring of 1962 had nearly everywhere Figure 1: Rhizome of iris aurantica. in Europe killed the flowers on the iris species. The only exception and a unique consolation, was the plants of Iris aurantica from GoldenSyria, which Iris gave Propagation us much joy with their flowers [5]. Iris species is usually accomplished vegetatively through bulbs or splitting of rhizomes (rhizomatous The propagation of Iris Iris, splitting the rhizomes gives a ). In rhizomatous the propagation of Iris maximum of 10 plants per year per rhizome [8]. Furthermore, species through seedlings is known to be difficult due to a poor fruit set and a very low germination rate. propagation and conservation of plants, especially for those that Plant tissue culture is a powerful alternative technique for are rare and difficult to propagate by conventional methods. Figure 2: Leaves and roots of iris aurantica. Therefore, a new trend has evolved to propagate these species deterioration and to study the possibility of using them as a through tissue culture techniquein order to preserve it from Iris aurantica has green falcate leaves, they can grow up to medicalMicro or propagation ornamental plant.is the aseptic culture of cells, pieces of 15-25cm long and between 1-1.5cm wide (Figure 2). Leaves can small pieces of plant tissue identical to the plant from which it sheath up to haft of the stem after the iris has bloomed. At the tissue, or organs. It is possible to regenerate new plants from summer sun with dry conditions, leaves fade and die. Golden Iris has a slender stem, which can grow up to 30-50cm tall. The was derived. stages: stems hold terminal flowers (top of stem), blooming during May The process of micro propagation can be divided into four and Jun, they can flower for up of month long [4]. A. Initiation stage: The objective of this stage is to achieve an aseptic culture. An aseptic culture is one without of rhizomes in Iris aurantica (after surface disinfection by contaminating bacteria or fungi. Base of leaves and shoot tips chlorox 3%) were cultured on solidified MS medium containing 30g/l sucrose, and supplemented with 2mg/lBAP and 0.2mg/ month of culture, that using shoot tips of rhizomes resulted in lIBA (Abouzedan and Al-Batal 2015). Results showed, after one the highestB. Multiplication growth percentage stage: (35.76%) A growing in initial ex plantstage [9].can be Figure 3: Iris aurantica flower parts. Iris aurantica, the highest induced to produce vegetative shoots by including a cytokinin Golden Iris has a slender stem, which can grow up to 30- in the medium and different media. In average number of shoots per ex plant was found (3.43) under 50cm tall. The stems hold terminal flowers (top of stem), resulted in the highest multiplication rate and shoot length with blooming during May and Jun, they can flower for up of month BAP at a concentration of 3.0mg/l (Figure 4), and MS media long [4]. The flowers like other irises, it has two pairs of petals, of the plantlets on the same medium resulted in increasing significant difference compared with Heller media, Subculture three large sepals, known as the falls and three inner smaller multiplication rate and shoot length in Iris aurantica petals or sepals, known as the standards. The standards are [9] the How to cite this article: Khalil AL M, Thuraya A z, Nabil AL B. Wild Golden Iris (Iris aurantica) in Syria. Curr Trends Biomedical Eng & Biosci. 2017; 1(5): 00112 555573. Current Trends in Biomedical Engineering & Biosciences acclimatization in vivo was achieved easily with high percentage treatment of high concentrations of cytokinins (5 and 10mg/ tissue-cultured plants are extremely susceptible to wilting. The iris aurantica lBAP) consist of appearance of vitrification. later, plantlets were cultured in greenhouse and the average of success (86.95%) in (Figure 6). Two months, length of shoots were 23. 25 cm [10]. Figure 4: Multiplication stage in Iris aurantica. Figure 6: Acclimatization stage. A: plantlet after one week, B: plantlet after 2 months, C: plantlets Growing shoots can be induced C. Rooting stage: in greenhouse. to produce adventitious roots by including an auxin in the In iris aurantica In Vitro Conservation medium. , the highest root percentage (88.5%) was obtained on medium containing 3mg/lIBA (Figure 5). conservation plants material for periods ranging from 6 months Conservation is a very simple in vitro technique that permits The highest root number (4.25) was recorded when using the concentration 0.5mg/lIBA [9] (Table 1). on reducing the growth rates of the tissue cultured plant and to 5-7 years, depending on species [11]. This technique is based was conducted, to develop an in vitro yet increasing the intervals between subcultures [12]. Research conservation and relieve of growth and increase the period technique for short-term of time between transfers of Iris aurantica agents for in vitro conservation was sucrose compared with , The best osmotic iris aurantica (mannitol and sorbitol) and the best medium concentration was 1/10 MS [10] in the cultured stored at (3 ˚C) gave the highest survival (93.33%) and lengthening the time to regulate expression of many genes that are responsible period between transfers up to 6 months [10].