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Jeffrey Evan Barrick

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Jeffrey Evan Barrick Jeffrey Evan Barrick 2500 Speedway A5000 University of Texas at Austin Austin, TX 78712 phone: (512) 471-3247 email: [email protected] website: http://barricklab.org Education California Institute of Technology (1997–2001) B.S. Chemistry with Honors Yale University (2001–2006) Ph.D. Molecular Biochemistry and Biophysics Professional Experience Associate Professor (2018–present) The University of Texas at Austin Department of Molecular Biosciences (previously Chemistry & Biochemistry) Institute for Cellular and Molecular Biology Fellow of the Lorene Morrow Kelley Professorship Center for Systems and Synthetic Biology Center for Computational Biology and Bioinformatics Ecology, Evolution and Behavior Graduate Program NSF BEACON Center for the Study of Evolution in Action Assistant Professor (2011–2018) The University of Texas at Austin Department of Molecular Biosciences (previously Chemistry & Biochemistry) Postdoctoral Fellow (2006–2010) Michigan State University (Richard E. Lenski and Charles A. Ofria) Graduate Student (2001–2006) Yale University (Ronald R. Breaker) Title of Dissertation Discovering and defining metabolite-binding riboswitches and other structured regulatory RNA motifs in bacteria 1 Dissertation Advisor Ronald R. Breaker Postdoctoral Research Topics • E. coli experimental evolution and genomics • Digital evolution and artificial life Postdoctoral Advisors Richard. E. Lenski and Charles A. Ofria (co-advised) Current Research Interests Controlling evolution is key to combating the emergence of drug resistance, preventing the progression of chronic infections and cancers, and maintaining the function of engineered cells. Evolution can also be directed and harnessed for biotechnology and medicine, to create novel therapeutics and optimize microbial strains, for example. My lab uses experimental approaches that leverage the tools of synthetic and systems biology to study and manipulate evolution. Our current interests are focused on (1) understanding microbial genome dynamics during adaptive laboratory evolution experiments; (2) dissecting the molecular mechanisms of metabolic innovation and optimization during adaptive evolution; (3) examining how microbes evolve on a whole-genome level when they gain access to genetic codes with new amino acid building blocks; (4) developing antimutator strains and other methods for preventing undesirable evolution when it causes failures in biological engineering; and (5) genetically engineering bacterial symbionts of insects for applications in agriculture. Professional Societies Sigma Xi (2001–present) Society for the Study of Evolution (2008-present) Society for Molecular Biology and Evolution (2009–present) American Chemical Society (2010–present) American Society for Microbiology (2011–present) International Society for Artificial Life (2012-present) Society for Biological Engineering (2014–present) Genetics Society of America (2017–present) Grants Current Funding PI NSF ENG/BIO (CBET-1554179) CAREER: Preventing evolutionary failure in synthetic biology; PI: Barrick; $500,000 total costs; 05/15/2016–05/14/2021. 2 DARPA Insect Allies (HR001117S0002-IA-FP-008) AEPHID: Aphid Endosymbionts for Plant Host Immunization and Defense PI: Barrick 35% contribution; co-PI: Nancy Moran (The University of Texas at Austin) 25% contribution; co-PI: Andrew Ellington (The University of Texas at Austin) 25% contribution; co-PI: Thomas McKnight (Texas A&M University) 15% contribution; $5,029,456 total costs; ~$1,800,000 to Barrick; 07/01/2017–06/30/2021. UT Austin Vice President for Research Office (Associate Professor Experimental) APX: 3D-Printed Observatories for Visualizing and Accelerating Evolution; PI: Barrick 50% contribution; co-PI: Kory Bieg (The University of Texas at Austin) 50% contribution; co-PI: Robert Newman (North Carolina A&T) 25% contribution; $100,000 total costs; $37,400.00 to Barrick; 11/01/2018–12/31/2019. Welch Foundation (F-1979) Chemical specificity of DNA uptake by naturally competent bacteria; PI: Barrick; $195,000 total costs; 06/01/2018–05/31/2021. NSF BEACON Center (DBI-0939454 subcontract) Standardizing measurements of evolutionary stability parameters in synthetic biology; PI: Barrick 40% contribution; co-PI: Dennis Mishler (The University of Texas at Austin) 10% contribution; co-PI Daniel Ducat (Michigan State University) 13% contribution; Björn Hamburger (Michigan State University) 14%; contribution; Michaela TerAvest (Michigan State University) 13% contribution; $84,146 total costs; $44,320 to Barrick; 06/01/2019– 05/31/2020. Co-PI NSF US-Israel Collab: Are parasites in complex communities more evolvable? Bridging ecology and evolution with computational modeling and rodent-bacteria evolution experiments; PI: Luis Zaman (University of Michigan) 50% contribution; co-PI: Barrick 35% contribution; co-PI: Richard Lenski (Michigan State University) 15% contribution. $1,594,591 total costs: $482,016 to Barrick; 09/01/2018- 08/31/2023. Previous Funding PI NIH NIGMS (R00) Identifying mutations that promote microbial evolvability; PI: Barrick; $1,250,000 total costs; 02/01/2011–01/31/2014. Welch Foundation (F-1780) Discovering functional nucleic acid families by deep sequencing and fold sampling; PI: Barrick; $180,000 total costs; 06/01/2012– 05/31/2015. 3 Cancer Prevention & Research Institute of Texas (RP130124) Deep sequencing oncoviral evolvomes as a personalized cancer diagnostic tool; PI: Barrick; $260,633 total costs; 06/01/2013–11/30/2015. NSF BEACON Center (DBI-0939454 subcontract); Evolutionary origins and engineering applications of bacterial DNA secretion machinery; PI: Barrick; $42,940 total costs; 06/01/2013–05/31/2014. UT Austin (Research Grant) Probing the molecular specificity of natural competence in Acinetobacter baylyi ADP1; PI: Barrick; $6,000 total costs; 10/15/2013–08/31/2014. NSF BEACON Center (DBI-0939454 subcontract); Cross-institutional iGEM synthetic evolutionary biology team; PI: Barrick 40% contribution; co-PIs: Andrew Ellington (The University of Texas at Austin) 5% contribution; Robert Newman (North Carolina A&T State University) 35% contribution; Randall Hayes (North Carolina A&T State University) 5% contribution; Scott Harrison (North Carolina A&T State University) 5% contribution; Gregory Goins (North Carolina A&T State University) 5% contribution; Jian Han (North Carolina A&T State University) 5% contribution; $48,839 total costs; $19,912 to Barrick; 06/01/2013–05/31/2014. NSF BEACON Center (DBI-0939454 subcontract); Illuminating evolution in action with an expanded genetic code; PI: Barrick 50% contribution; co-PI: Robert Newman (North Carolina A&T State University) 30% contribution; co-PI Scott Harrison (North Carolina A&T State University) 20% contribution; $170,136 total costs; $105,204 to Barrick; 06/01/2013–05/31/2015 NSF BEACON Center (DBI-0939454 subcontract); Mechanistic basis of mutations potentiating the evolution of citrate utilization in the LTEE; PI: Barrick 40% contribution; co-PI: Christopher Marx (University of Idaho) 30% contribution; co-PI: Zachary Blount (Michigan State University) 30% contribution; $134,570 total costs; $54,185 to Barrick. 05/01/2014–04/30/2015. NSF BEACON Center (DBI-0939454 subcontract); Directed evolution of novel protein functions using expanded genetic codes; PI: Barrick 40% contribution; co- PI: Bryan Davies (The University of Texas at Austin) 20% contribution; Robert Newman (North Carolina A&T State University) 40% contribution; $131,524 total costs; $34,732 to Barrick; 05/01/2015–04/30/2016. NSF BEACON Center (DBI-0939454 subcontract); Evolution of cultured stem cells; PI: Barrick 25% contribution; co-PI: Austin Cooney (The University of Texas at Austin) 40% contribution; co-PI: Holly Wichman (University of Idaho) 25% contribution; co-PI James Bull (The University of Texas at Austin) 10% contribution; $83,352 total costs, $20,450 to Barrick; 05/01/2016–04/30/2017. 4 NSF I-Corps (IIP-1744677) Diagnosing the mutational failure modes of cells so that biotech companies can shorten R&D time and optimize production PI: Barrick; $50,000 total costs; 06/01/2017–5/31/2018. NSF BEACON Center (DBI-0939454 subcontract) Decoy mutations: engineering fitness landscapes to delay unwanted evolution; PI: Barrick 50% contribution; co-PI: Luis Zaman (University of Michigan) 30% contribution; co-PI: Christoph Adami (Michigan State University) 20% contribution; $59,368 total costs; $23,125 to Barrick; 06/01/2017–05/31/2018. NSF BEACON Center (DBI-0939454 subcontract) Detecting unplanned evolution in plasmid repositories; PI: Barrick 50% contribution; co-PI: Eva Top (University of Idaho) 25% contribution; co-PI: Robert Newman (North Carolina A&T) 25% contribution; $87,127 total costs; $39,075 to Barrick; 06/01/2018–05/31/2019. NSF BEACON Center (DBI-0939454 subcontract) Multiplex measurement of mutation rates and spectra; PI: Barrick 50% contribution; co-PI: Maitreya Dunham (University of Washington, Seattle) 50% contribution; $84,770 total costs; $46,167 to Barrick; 06/01/2015–05/31/2018. Welch Foundation (F-1780) Comparing the chemical utility of alternative genetic codes; PI: Barrick; $195,000 total costs; 06/01/2015–05/31/2018. Co-PI NSF BEACON Center (DBI-0939454 subcontract); Reducing yeast evolvability; PI: Maitreya Dunham (University of Washington, Seattle) 55% contribution; co-PI: Barrick 45% contribution; $61,667 total costs; $28,938 to Barrick; 05/01/2016– 04/30/2017. NSF BEACON Center (DBI-0939454 subcontract); Using evolution to explore antimicrobial resistance and spur new antimicrobial
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