bioRxiv preprint doi: https://doi.org/10.1101/2020.06.02.130138; this version posted June 3, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Maintenance of Metabolic Plasticity Despite Relaxed Selection in a Long-Term Evolution Experiment with Escherichia coli Nkrumah A. Grant,1,2,3* Rohan Maddamsetti,4 and Richard E. Lenski1,2,3 1. BEACON Center for the Study of Evolution in Action, Michigan State University, East Lansing, Michigan 48824; 2. Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824; 3. Program in Ecology, Evolutionary Biology and Behavior, Michigan State University, East Lansing 48824; 4. Department of Biomedical Engineering, DuKe University, Durham, North Carolina 27708. * Corresponding author; e-mail:
[email protected] 1 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.06.02.130138; this version posted June 3, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 2 ABSTRACT: Traits that are unused in a given environment are subject to processes that tend to erode 3 them, leading to reduced fitness in other environments. Although this general tendency is clear, 4 we know much less about why some traits are lost while others are retained, and about the roles of 5 mutation and selection in generating different responses.