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Phytochemical and Pharmacological Study of Hypericum Triquetrifolium

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Phytochemical and Pharmacological Study of Hypericum Triquetrifolium Phytochemical and pharmacological study of Hypericum triquetrifolium TURRA Dissertation submitted to the Faculty of Chemistry and Pharmacy, University of Regensburg for the degree of Doctor of Natural Sciences (Dr. rer. nat.) presented by Ilya B. Volkov from Holon, Israel 2017 The present work was carried out from October 2013 until October 2016 under the supervision of Prof. Dr. Jörg Heilmann at the Pharmaceutical Biology department, Institute of Pharmacy, Faculty of Chemistry and Pharmacy, University of Regensburg. Dissertation submitted on: 2nd December, 2016 Day of oral presentation: 10th February, 2017 Examination committee: Prof. Dr. Sigurd Elz (Chairperson) Prof. Dr. Jörg Heilmann (First examiner) Prof. Dr. Michael Decker (Second examiner) PD Dr. Birgit Kraus (Third examiner) Fortune favours the bold. ― Virgil, “The Aeneid” Acknowledgements My deepest and most sincere thank you first goes to Prof. Dr. Jörg Heilmann for accepting me to your working group. It was a great pleasure to work on your department, to learn tons of new things, not only biological, chemical, biochemical, analytical, pharmaceutical, etc., but also to discover a whole new world all around me. I am extremely grateful for your kindness, support, taking time, sharing ideas, explanations and the fact you did care. PD Dr. Birgit Kraus, many thanks for taking your time and, what is much more important, patience, for teaching and explaining how things in cell culture work. It was an unforgettable experience and I loved it a lot. Thank you for your inspiration, I do highly appreciate that. Dr. habil. Guido Jürgenliemk, thank you for leading me in the right direction, both during the studies and during the Bioanalytik Praktikum. Thank you for your kind and fruitful advises, knowledge and willingness to help. Dr. Sebastian Schmidt, without you all this would not have been possible. Thank you for your patience, wise advises, for sharing attainments, thoughts and experience. I’m also grateful for your kind help in proofreading! Gabi Brunner, for keeping everything in perfect and strict order and letting know where the things are. The Department never functions the same without you! Thank you for open column teaching and taking time in explanations about useful cell culture tricks, as well as insuring me with extra cells available. Anne Grasshuber for helping in practice with students, for your kindness and enormous help by extraction. I wish you nothing, but the very best! Hedwig Ohli, our dear secretary, for helping in being accepted to the department, for helping with the accommodation, for all the messy but necessary administrative things. Central Analytical Department of the University of Regensburg: Fritz Kastner and Dr. Ilya Schenderowitch for taking NMR spectra; Josef Kiermaier and Wolfgang Söllner for taking MS spectra. PD Dr. Axel Dürkop (Institute of Analytical Chemistry, Chemo- and Biosensorik) for measuring CD-spectra and telling stories about the Uni’s history. Mrs. Michal Monosov (Hebrew University Botanical Gardens on Mt. Scopus, Jerusalem) and Mrs. Hagar Leschner (The Israeli National Herbarium, Hebrew University, Givat-Ram, Jerusalem) for your magical and kind help in finding, verifying and transporting the herb material to Germany. My dearest lab Mädls – Katharina Schiller and Eva Lotter for all your help, sincere laughs, Bavarian language and tradition lessons (I red a bissl Boarisch und des is a guad so! ), Budapest drinks and walks, cartoons, die 3 Besoffskis, TV commercials, Christmas songs, Regina & Garbo Filmtheaters, etc. for creating the coolest lab spirit one could have ever dreamt to have – you are unequivocally a true enco-co-couraging! Julianna Ziegler, for joint bus trips to Munich, advises and notes along the work and proofreading! I wish you all the good luck with your Hypericum sp.! Dr. Edna E. Makule (Arusha, Tanzania), for your inspiration and advices, for patience, help and explanations, for always bringing the sun with you. Very much appreciated! Sebastian Schwindl, for your recommendations and discoveries in cell culture, NMR and optical rotation. My department colleagues Christian Zeh, Stefan Wiesneth, Sina Malenke, Dr. Monika Untergehrer, Dr. Tri-Hieu Nguen (Vietnam), Dr. Beata Kling, Dr. Marcel Flemming, Dr. Petr Jirásek, Dr. Rosi Scherübl – thank you for the inspiration, it was a great pleasure to work with you! Sebastian Pitzl, thank you very much for your suggestions and permission to reprint the unpublished data of your master thesis for comparison reasons. Good luck with your PhD work! Our international guest scientists Carlo and Sylvia (Vinece, Italy), Angeliki, Arthemis and Ariola (Athens, Greece) and Filip (Novi Sad, Serbia). I hope you had a lot of fun with 2D-NMR spectra! Dr. Parsekev Nedialkov (Sofia, Bulgaria) and Dr. Peter Lorenz from „Wala“ for great time at the GA-2015 conference in Budapest, for fruitful discussions and wise advises. My interns Svetlana Reichert, Rafael Rager and Marcell Kaljanac for teaching me something new. I am extremely thankful to all the people, who inspired and supported me mentally during these years and helped me in performing this work. Among them, almighty and unbeliveable Per Gessle, charming and cute Helena Josefsson, kind and touching Marie Fredriksson + Mazarinerna, SOAP and Gyllene Tider rockaboom guys and all the people from the Rox world, who have been lightening my everyday path during the recent 20 years. My gorgeous muse Eva Gaëlle Green and Susan Sarandon, Geoffrey Rush, Cate Blanchett, Sir Ian McKellen, Dame Judi Dench, Kevin Spacey, Tom Hanks and many others, who share their unlimited magic and spread the world with their sincere art and encouraged me all this time. My best friends Dr. Ilya Alexeev, Alexander Grigoruk and Sergey Dulin for your endless support and inspiration, for your ideas, honesty, warmth, sand of south oceans and spirit of Samui. I promise you to explore Sumatra one day and find Rafflesia arnoldii at last. My dearest Mr. Vladislav Braginsky for supporting me during all these years, for your kindness and understanding; I sincerely wish you all the good luck in everything you do. Frau Alexandra Gutzmer for friendly meows, cakes, photos, cafes and walks. Dr. med. dent. Vladimir and Ludmila Reboudé + the family for their financial and unlimited moral support. You have always been my back and I will never have enough words to thank you properly for what you have done for me. I extremely appreciate you being by my side. Dipl.-Ing. Zeev and Liora Kotel + the family for your wise advises and supporting my beginnings. For believing and not letting me alone, even if I was not right. My dearest parents, Dipl.-Ing. Boris and Irina Volkov (+ Manya) for your support, for your investments in my education, for taking care of me, for wishing me nothing but the best, for your endless love. The current work is dedicated to you. I would also like to thank my Eugen for your love, patience and support. I don’t know where I would’ve been without you. Thank you for your compassion and sincerity. I look forward to discover a whole new world together. Publications list Poster Ilya Volkov, Sebastian Schmidt, Jörg Heilmann (2015): Isolation and structure elucidation of acylphloroglucinols from Hypericum triquetrifolium TURRA. Planta Medica, 16(81): PM_85. DOI: 10.1055/s-0035-1565462. 63rd International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA2015). Budapest, Hungary, 23–27, August 2015 Content 1. Introduction………………………………………………………………………………………………………………1 1.1 Genus Hypericum L.…………………………………………………………………………………………….1 1.1.1 General information and classification……………………………………………………..1 1.1.2 Section 9 Hypericum L………………………………………………………………………………3 1.1.3 Hypericum triquetrifolium TURRA………………………………………………………………4 1.1.3.1 Geography and ecology………………………………………………………………………4 1.1.3.2 Etymology…………………………………………………………………………………………..5 1.1.3.3 Botanical and taxonomical description………………………………………….……6 1.1.3.4 Phytochemistry and pharmacology…………………………………………………..10 1.1.4 Acylphloroglucinols………………………………………………………………………………..12 1.1.4.1 Biosynthesis of acylphloroglucinols…………………………………………………..13 1.1.4.2 Structural diversity and classification of acylphloroglucinols…………….16 1.1.4.3 Biological properties……………………………………………………………………..….19 1.2 Neurodegenerative diseases…………………………………………………………………………….20 1.2.1 Introduction……………………………………………………………………………………………20 1.2.2 Aetiology of neurodegenerative disorders……………………………………………..21 1.2.3 Glutamate-induced neurotoxicity…………………………………………………………..25 1.2.4 Role of Hypericum in treating neurodegenerative disorders…………………..28 1.3 Aim of the present work……………………………………………………………………………………30 1.3.1 Isolation of acylphloroglucinols from H. triquetrifolium...........................30 1.3.2 Testing the isolated substances on neuroprotection………………………………30 2. Materials and methods…………………………………………………………………………………………..31 2.1 Phytochemical methods……………………………………………………………………………………31 2.1.1 Devices, vessels and software…………………………………………………………………31 2.1.2 Chemicals and solvents………………………………………………………………..…………32 2.1.3 Plant material………………………………………………………………………………….……..33 2.1.4 Extraction of plant material…………………………………………………………………...33 2.1.5 Fractioning and isolation………………………………………………………………………..34 2.1.5.1 Flash chromatography…………………………………………………….……………….34 2.1.5.2 Fractions control………………………………………………………………………..…….35 2.1.5.2.1 Thin-layer chromatography……………………………….………………….35 2.1.5.2.2 1H-NMR spectroscopy………………………………………………….……….37 2.1.5.3 Centrifugal
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