DOCSLIB.ORG

Phytochemical and Pharmacological Study of Hypericum Triquetrifolium

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text

Load more

Phytochemical and pharmacological study of Hypericum triquetrifolium TURRA Dissertation submitted to the Faculty of Chemistry and Pharmacy, University of Regensburg for the degree of Doctor of Natural Sciences (Dr. rer. nat.) presented by Ilya B. Volkov from Holon, Israel 2017 The present work was carried out from October 2013 until October 2016 under the supervision of Prof. Dr. Jörg Heilmann at the Pharmaceutical Biology department, Institute of Pharmacy, Faculty of Chemistry and Pharmacy, University of Regensburg. Dissertation submitted on: 2nd December, 2016 Day of oral presentation: 10th February, 2017 Examination committee: Prof. Dr. Sigurd Elz (Chairperson) Prof. Dr. Jörg Heilmann (First examiner) Prof. Dr. Michael Decker (Second examiner) PD Dr. Birgit Kraus (Third examiner) Fortune favours the bold. ― Virgil, “The Aeneid” Acknowledgements My deepest and most sincere thank you first goes to Prof. Dr. Jörg Heilmann for accepting me to your working group. It was a great pleasure to work on your department, to learn tons of new things, not only biological, chemical, biochemical, analytical, pharmaceutical, etc., but also to discover a whole new world all around me. I am extremely grateful for your kindness, support, taking time, sharing ideas, explanations and the fact you did care. PD Dr. Birgit Kraus, many thanks for taking your time and, what is much more important, patience, for teaching and explaining how things in cell culture work. It was an unforgettable experience and I loved it a lot. Thank you for your inspiration, I do highly appreciate that. Dr. habil. Guido Jürgenliemk, thank you for leading me in the right direction, both during the studies and during the Bioanalytik Praktikum. Thank you for your kind and fruitful advises, knowledge and willingness to help. Dr. Sebastian Schmidt, without you all this would not have been possible. Thank you for your patience, wise advises, for sharing attainments, thoughts and experience. I’m also grateful for your kind help in proofreading! Gabi Brunner, for keeping everything in perfect and strict order and letting know where the things are. The Department never functions the same without you! Thank you for open column teaching and taking time in explanations about useful cell culture tricks, as well as insuring me with extra cells available. Anne Grasshuber for helping in practice with students, for your kindness and enormous help by extraction. I wish you nothing, but the very best! Hedwig Ohli, our dear secretary, for helping in being accepted to the department, for helping with the accommodation, for all the messy but necessary administrative things. Central Analytical Department of the University of Regensburg: Fritz Kastner and Dr. Ilya Schenderowitch for taking NMR spectra; Josef Kiermaier and Wolfgang Söllner for taking MS spectra. PD Dr. Axel Dürkop (Institute of Analytical Chemistry, Chemo- and Biosensorik) for measuring CD-spectra and telling stories about the Uni’s history. Mrs. Michal Monosov (Hebrew University Botanical Gardens on Mt. Scopus, Jerusalem) and Mrs. Hagar Leschner (The Israeli National Herbarium, Hebrew University, Givat-Ram, Jerusalem) for your magical and kind help in finding, verifying and transporting the herb material to Germany. My dearest lab Mädls – Katharina Schiller and Eva Lotter for all your help, sincere laughs, Bavarian language and tradition lessons (I red a bissl Boarisch und des is a guad so! ), Budapest drinks and walks, cartoons, die 3 Besoffskis, TV commercials, Christmas songs, Regina & Garbo Filmtheaters, etc. for creating the coolest lab spirit one could have ever dreamt to have – you are unequivocally a true enco-co-couraging! Julianna Ziegler, for joint bus trips to Munich, advises and notes along the work and proofreading! I wish you all the good luck with your Hypericum sp.! Dr. Edna E. Makule (Arusha, Tanzania), for your inspiration and advices, for patience, help and explanations, for always bringing the sun with you. Very much appreciated! Sebastian Schwindl, for your recommendations and discoveries in cell culture, NMR and optical rotation. My department colleagues Christian Zeh, Stefan Wiesneth, Sina Malenke, Dr. Monika Untergehrer, Dr. Tri-Hieu Nguen (Vietnam), Dr. Beata Kling, Dr. Marcel Flemming, Dr. Petr Jirásek, Dr. Rosi Scherübl – thank you for the inspiration, it was a great pleasure to work with you! Sebastian Pitzl, thank you very much for your suggestions and permission to reprint the unpublished data of your master thesis for comparison reasons. Good luck with your PhD work! Our international guest scientists Carlo and Sylvia (Vinece, Italy), Angeliki, Arthemis and Ariola (Athens, Greece) and Filip (Novi Sad, Serbia). I hope you had a lot of fun with 2D-NMR spectra! Dr. Parsekev Nedialkov (Sofia, Bulgaria) and Dr. Peter Lorenz from „Wala“ for great time at the GA-2015 conference in Budapest, for fruitful discussions and wise advises. My interns Svetlana Reichert, Rafael Rager and Marcell Kaljanac for teaching me something new. I am extremely thankful to all the people, who inspired and supported me mentally during these years and helped me in performing this work. Among them, almighty and unbeliveable Per Gessle, charming and cute Helena Josefsson, kind and touching Marie Fredriksson + Mazarinerna, SOAP and Gyllene Tider rockaboom guys and all the people from the Rox world, who have been lightening my everyday path during the recent 20 years. My gorgeous muse Eva Gaëlle Green and Susan Sarandon, Geoffrey Rush, Cate Blanchett, Sir Ian McKellen, Dame Judi Dench, Kevin Spacey, Tom Hanks and many others, who share their unlimited magic and spread the world with their sincere art and encouraged me all this time. My best friends Dr. Ilya Alexeev, Alexander Grigoruk and Sergey Dulin for your endless support and inspiration, for your ideas, honesty, warmth, sand of south oceans and spirit of Samui. I promise you to explore Sumatra one day and find Rafflesia arnoldii at last. My dearest Mr. Vladislav Braginsky for supporting me during all these years, for your kindness and understanding; I sincerely wish you all the good luck in everything you do. Frau Alexandra Gutzmer for friendly meows, cakes, photos, cafes and walks. Dr. med. dent. Vladimir and Ludmila Reboudé + the family for their financial and unlimited moral support. You have always been my back and I will never have enough words to thank you properly for what you have done for me. I extremely appreciate you being by my side. Dipl.-Ing. Zeev and Liora Kotel + the family for your wise advises and supporting my beginnings. For believing and not letting me alone, even if I was not right. My dearest parents, Dipl.-Ing. Boris and Irina Volkov (+ Manya) for your support, for your investments in my education, for taking care of me, for wishing me nothing but the best, for your endless love. The current work is dedicated to you. I would also like to thank my Eugen for your love, patience and support. I don’t know where I would’ve been without you. Thank you for your compassion and sincerity. I look forward to discover a whole new world together. Publications list Poster Ilya Volkov, Sebastian Schmidt, Jörg Heilmann (2015): Isolation and structure elucidation of acylphloroglucinols from Hypericum triquetrifolium TURRA. Planta Medica, 16(81): PM_85. DOI: 10.1055/s-0035-1565462. 63rd International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA2015). Budapest, Hungary, 23–27, August 2015 Content 1. Introduction………………………………………………………………………………………………………………1 1.1 Genus Hypericum L.…………………………………………………………………………………………….1 1.1.1 General information and classification……………………………………………………..1 1.1.2 Section 9 Hypericum L………………………………………………………………………………3 1.1.3 Hypericum triquetrifolium TURRA………………………………………………………………4 1.1.3.1 Geography and ecology………………………………………………………………………4 1.1.3.2 Etymology…………………………………………………………………………………………..5 1.1.3.3 Botanical and taxonomical description………………………………………….……6 1.1.3.4 Phytochemistry and pharmacology…………………………………………………..10 1.1.4 Acylphloroglucinols………………………………………………………………………………..12 1.1.4.1 Biosynthesis of acylphloroglucinols…………………………………………………..13 1.1.4.2 Structural diversity and classification of acylphloroglucinols…………….16 1.1.4.3 Biological properties……………………………………………………………………..….19 1.2 Neurodegenerative diseases…………………………………………………………………………….20 1.2.1 Introduction……………………………………………………………………………………………20 1.2.2 Aetiology of neurodegenerative disorders……………………………………………..21 1.2.3 Glutamate-induced neurotoxicity…………………………………………………………..25 1.2.4 Role of Hypericum in treating neurodegenerative disorders…………………..28 1.3 Aim of the present work……………………………………………………………………………………30 1.3.1 Isolation of acylphloroglucinols from H. triquetrifolium...........................30 1.3.2 Testing the isolated substances on neuroprotection………………………………30 2. Materials and methods…………………………………………………………………………………………..31 2.1 Phytochemical methods……………………………………………………………………………………31 2.1.1 Devices, vessels and software…………………………………………………………………31 2.1.2 Chemicals and solvents………………………………………………………………..…………32 2.1.3 Plant material………………………………………………………………………………….……..33 2.1.4 Extraction of plant material…………………………………………………………………...33 2.1.5 Fractioning and isolation………………………………………………………………………..34 2.1.5.1 Flash chromatography…………………………………………………….……………….34 2.1.5.2 Fractions control………………………………………………………………………..…….35 2.1.5.2.1 Thin-layer chromatography……………………………….………………….35 2.1.5.2.2 1H-NMR spectroscopy………………………………………………….……….37 2.1.5.3 Centrifugal
Recommended publications
  • GROUND COVERS for KENTUCKY LANDSCAPES Lenore J

    GROUND COVERS for KENTUCKY LANDSCAPES Lenore J

    HO-78 C O O P E R A T I V E E X T E N S I O N S E R V I C E U N I V E R S I T Y O F K E N T U C K Y • C O L L E G E O F A G R I C U L T U R E GROUND COVERS FOR KENTUCKY LANDSCAPES Lenore J. Nash, Mary L. Witt,William M. Fountain, Robert L. Geneve “Ground cover” is a term that describes a wide variety Color and texture offered by ground covers give the of plants useful for special planting situations. A common designer additional choices. There is a wide array of characteristic of all ground covers is uniform growth that foliage textures and colors, as well as seasonal flowers and covers the ground with enough density to compete well showy fruit. with weedy plants. Ground covers may function as traffic barriers Naturally-occurring ground covers are a delight, because they do not invite you to walk on them as turf although we may often miss the fact that they are indeed grasses do, yet they are low enough not to be a sight serving as ground covers. Think of mixed assortments of barrier. In this capacity, they give the added benefit of perennial flowers and ferns in a wooded area or snowberry keeping lawnmowers and string trimmers away from blanketing steep slopes along road cuts. valuable woody and herbaceous plants. Ground covers are valuable in special sites where turf grass will not thrive, where regular turf maintenance (mow- Soil ing) is a problem, or where a diversity of color and texture Ground covers grow in close proximity, so well- are desirable.
  • Explosive Radiation in High Andean Hypericum—Rates of Diversification

    Explosive Radiation in High Andean Hypericum—Rates of Diversification

    ORIGINAL RESEARCH ARTICLE published: 11 September 2013 doi: 10.3389/fgene.2013.00175 Explosive radiation in high Andean Hypericum—rates of diversification among New World lineages Nicolai M. Nürk 1*, Charlotte Scheriau 1 and Santiago Madriñán 2 1 Department of Biodiversity and Plant Systematics, Centre for Organismal Studies Heidelberg, Heidelberg University, Heidelberg, Germany 2 Laboratorio de Botánica y Sistemática, Departamento de Ciencias Biológicas, Universidad de los Andes, Bogotá DC, Colombia Edited by: The páramos, high-elevation Andean grasslands ranging from ca. 2800 m to the snow Federico Luebert, Freie Universität line, harbor one of the fastest evolving biomes worldwide since their appearance in the Berlin, Germany northern Andes 3–5 million years (Ma) ago. Hypericum (St. John’s wort), with over 65% Reviewed by: of its Neotropical species, has a center of diversity in these high Mountain ecosystems. Andrea S. Meseguer, Institute National de la research agricultural, Using nuclear rDNA internal transcribed spacer (ITS) sequences of a broad sample of France New World Hypericum species we investigate phylogenetic patterns, estimate divergence Colin Hughes, University of Zurich, times, and provide the first insights into diversification rates within the genus in the Switzerland Neotropics. Two lineages appear to have independently dispersed into South America *Correspondence: around 3.5 Ma ago, one of which has radiated in the páramos (Brathys). We find strong Nicolai M. Nürk, Department of Biodiversity and Plant Systematics, support for the polyphyly of section Trigynobrathys, several species of which group within Centre for Organismal Studies Brathys, while others are found in temperate lowland South America (Trigynobrathys Heidelberg, Heidelberg University, s.str.).
  • Native Plants to Support Bumblebees at Risk

    Native Plants to Support Bumblebees at Risk

    Research-based native plants to support MA bumblebee species at risk. Plants supporting butterfly and other bee species at risk are also indicated. List created by Dr. Robert J Gegear, UMASS Dartmouth ([email protected]). Pollen Sources 'At risk' species BLOOM TIME BUMBLEBEES BUTTER OTHER LATIN NAME COMMON NAME PLANT TYPE SUN SOIL Early Mid Late B. B. B. FLIES BEES MAMJJASOterricola fervidus vagans Hypericum ascyron Great St. John’s-wort Herb. perenn. Full-Part Med NA Hypericum majus Greater St. John’s-wort Herb. perenn. Full-Part Med, Wet NA Hypericum punctatum Spotted St. John’s-wort Herb. perenn. Full-Part Med, Dry NA Hypericum prolificum Shrubby St. John’s-wort Shrub Full-Part Med, Dry NA Rosa acicularis Bristly rose Shrub Full-Shade Med, Dry NA Rosa blanda Smooth rose Shrub Full-Part Med, Dry NA Wet, Med, Rosa carolina Carolina rose Shrub Full-Part NA Dry Rosa nitida Shining rose Shrub Full-Part Wet, Med NA Rosa palustris Swamp rose Shrub Full-Part Med NA Rosa virginiana Virginia rose Shrub Full-Part Med NA Rubus odoratus Flowering raspberry Shrub Full-Part Med NA Salix discolor Pussy willow (male) Shrub Full Med NA Salix humilis Prairie willow (male) Shrub Full-Part Med, Dry NA Salix lucida Shining willow (male) Shrub Full-Part Med, wet NA Salix occidentalis Dwarf prairie willow (male) Shrub Full-Part Med, Dry NA Salix petiolaris Meadow willow (male) Shrub/sm tree Full-Part Med NA Dry, Med, Salix bebbiana Bebb willow (male) Shrub Full-Part NA Wet Spiraea alba White meadowsweet Shrub Full-Part Wet, Med NA Spiraea tomentosa Steeplebush Shrub Full-Part Wet NA BLOOM TIME BUMBLEBEES Nectar sources BUTTER OTHER Early Mid Late LATIN NAME COMMON NAME PLANT TYPE SUN SOIL B.
  • St. John's Wort 2018

    St. John's Wort 2018

    ONLINE SERIES MONOGRAPHS The Scientific Foundation for Herbal Medicinal Products Hyperici herba St. John's Wort 2018 www.escop.com The Scientific Foundation for Herbal Medicinal Products HYPERICI HERBA St. John's Wort 2018 ESCOP Monographs were first published in loose-leaf form progressively from 1996 to 1999 as Fascicules 1-6, each of 10 monographs © ESCOP 1996, 1997, 1999 Second Edition, completely revised and expanded © ESCOP 2003 Second Edition, Supplement 2009 © ESCOP 2009 ONLINE SERIES ISBN 978-1-901964-61-5 Hyperici herba - St. John's Wort © ESCOP 2018 Published by the European Scientific Cooperative on Phytotherapy (ESCOP) Notaries House, Chapel Street, Exeter EX1 1EZ, United Kingdom www.escop.com All rights reserved Except for the purposes of private study, research, criticism or review no part of this text may be reproduced, stored in a retrieval system or transmitted, in any form or by any means, without the written permission of the publisher. Important Note: Medical knowledge is ever-changing. As new research and clinical experience broaden our knowledge, changes in treatment may be required. In their efforts to provide information on the efficacy and safety of herbal drugs and herbal preparations, presented as a substantial overview together with summaries of relevant data, the authors of the material herein have consulted comprehensive sources believed to be reliable. However, in view of the possibility of human error by the authors or publisher of the work herein, or changes in medical knowledge, neither the authors nor the publisher, nor any other party involved in the preparation of this work, warrants that the information contained herein is in every respect accurate or complete, and they are not responsible for any errors or omissions or for results obtained by the use of such information.
  • Utilizing Novel Grasslands for the Conservation and Restoration Of

    Utilizing Novel Grasslands for the Conservation and Restoration Of

    Iowa State University Capstones, Theses and Graduate Theses and Dissertations Dissertations 2014 Utilizing novel grasslands for the conservation and restoration of butterflies nda other pollinators in agricultural ecosystems John Thomas Delaney Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/etd Part of the Biodiversity Commons, Ecology and Evolutionary Biology Commons, Natural Resources and Conservation Commons, and the Natural Resources Management and Policy Commons Recommended Citation Delaney, John Thomas, "Utilizing novel grasslands for the conservation and restoration of butterflies and other pollinators in agricultural ecosystems" (2014). Graduate Theses and Dissertations. 14097. https://lib.dr.iastate.edu/etd/14097 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Utilizing novel grasslands for the conservation and restoration of butterflies and other pollinators in agricultural ecosystems by John Thomas Delaney A dissertation submitted to the graduate faculty in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Major: Ecology and Evolutionary Biology Program of Study Committee: Diane M. Debinski, Major Professor David M. Engle Mary A. Harris Amy L. Toth Brian J. Wilsey Iowa State University Ames, Iowa 2014 Copyright © John Thomas Delaney, 2014. All rights reserved. ii Dedication I dedicate this dissertation to all of my family, friends, and mentors who have helped me along in this journey.
  • Mindy Goldman, MD Clinical Professor Dept

    Mindy Goldman, MD Clinical Professor Dept

    Managing Menopause Medically and Naturally Mindy Goldman, MD Clinical Professor Dept. of Ob/Gyn and Reproductive Sciences Director, Women’s Cancer Care Program, UCSF Breast Care Center and Women’s Health University of California, San Francisco I have nothing to disclose –Mindy Goldman, MD CASE STUDY 50 yr. old G2P2 peri-menopausal woman presents with complaints of significant night sweats interfering with her ability to sleep. She has mild hot flashes during the day. She has never had a bone mineral density test but her mother had a hip fracture at age 62 due to osteoporosis. Her 46 yr. old sister was diagnosed with breast cancer at age 43, treated with lumpectomy and radiation and currently is doing well. There is no other family history of cancer. Questions 1. Would you offer her MHT? 2. If yes, how long would you continue it? 3. If no, what would you offer for alternative treatments? 4. Would your treatment differ if you knew she had underlying heart disease? Is it safe? How long can I take it? What about Mymy Bones?bones? Will it protect my heart? MHT - 2015 What about my brain? Will I get breast cancer? What about my hot flashes? Menopausal Symptoms Hot flashes Night sweats Sleep disturbances Vaginal dryness/Sexual dysfunction Mood disturbances How to Treat Menopausal Symptoms Hormone therapy Alternatives to hormones Complementary and Integrative Techniques Prior to Women’s Health Initiative Hormone therapy primary treatment of menopausal hot flashes Few women would continue hormones past one year By 1990’s well known
  • Antiproliferative Effects of St. John's Wort, Its Derivatives, and Other Hypericum Species in Hematologic Malignancies

    Antiproliferative Effects of St. John's Wort, Its Derivatives, and Other Hypericum Species in Hematologic Malignancies

    International Journal of Molecular Sciences Review Antiproliferative Effects of St. John’s Wort, Its Derivatives, and Other Hypericum Species in Hematologic Malignancies Alessandro Allegra 1,* , Alessandro Tonacci 2 , Elvira Ventura Spagnolo 3, Caterina Musolino 1 and Sebastiano Gangemi 4 1 Division of Hematology, Department of Human Pathology in Adulthood and Childhood “Gaetano Barresi”, University of Messina, 98125 Messina, Italy; [email protected] 2 Clinical Physiology Institute, National Research Council of Italy (IFC-CNR), 56124 Pisa, Italy; [email protected] 3 Section of Legal Medicine, Department of Health Promotion Sciences, Maternal and Infant Care, Internal Medicine and Medical Specialties (PROMISE), University of Palermo, Via del Vespro, 129, 90127 Palermo, Italy; [email protected] 4 School and Operative Unit of Allergy and Clinical Immunology, Department of Clinical and Experimental Medicine, University of Messina, 98125 Messina, Italy; [email protected] * Correspondence: [email protected]; Tel.: +39-090-221-2364 Abstract: Hypericum is a widely present plant, and extracts of its leaves, flowers, and aerial elements have been employed for many years as therapeutic cures for depression, skin wounds, and respiratory and inflammatory disorders. Hypericum also displays an ample variety of other biological actions, such as hypotensive, analgesic, anti-infective, anti-oxidant, and spasmolytic abilities. However, recent investigations highlighted that this species could be advantageous for the cure of other pathological situations, such as trigeminal neuralgia, as well as in the treatment of cancer. This review focuses on the in vitro and in vivo antitumor effects of St. John’s Wort (Hypericum perforatum), its derivatives, and other Hypericum species in hematologic malignancies.
  • Functional Characterization of Prenyltransferases Involved in the Biosynthesis of Polycyclic Polyprenylated Acylphloroglucinols in the Genus Hypericum

    Functional Characterization of Prenyltransferases Involved in the Biosynthesis of Polycyclic Polyprenylated Acylphloroglucinols in the Genus Hypericum

    Functional characterization of prenyltransferases involved in the biosynthesis of polycyclic polyprenylated acylphloroglucinols in the genus Hypericum Von der Fakultät für Lebenswissenschaften der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung des Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) genehmigte D i s s e r t a t i o n von Mohamed Mamdouh Sayed Nagia aus Kalyobiya/ Ägypten 1. Referent: Professor Dr. Ludger Beerhues 2. Referent: Professor Dr. Alain Tissier eingereicht am: 30.07.2018 mündliche Prüfung (Disputation) am: 15.10.2018 Druckjahr 2018 „Gedruckt mit Unterstützung des Deutschen Akademischen Austauschdienstes“ „Und sag: O mein Herr, mehre mein Wissen“ Der Edle Qur’an [20: 114] Vorveröffentlichungen der Dissertation Teilergebnisse aus dieser Arbeit wurden mit Genehmigung der Fakultät für Lebenswissenschaften, vertreten durch den Mentor der Arbeit, in folgenden Beiträgen vorab veröffentlicht: Publikationen Nagia, M., Gaid, M., Biedermann, E., Fiesel, T., El-Awaad, I., Haensch, R., Wittstock, U., and Beerhues, L. Sequential regiospecific gem-diprenylation of tetrahydroxyxanthone by prenyltransferases from Hypericum sp. (Submitted). Nagia, M., Gaid, M., Beuerle, T., and Beerhues, L. Successive xanthone prenylation in Hypericum sampsonii. Planta Medica International Open 4, Tu-SL-01 (2017). doi: 10.1055/s-0037-1608308 Tagungsbeiträge A. Vorträge Nagia M., Gaid M., Biedermann E., Beuerle T., Beerhues L., Successive xanthone prenylation in Hypericum sampsonii, 65th Annual Meeting of the Society for Medicinal Plant and Natural Product Research, Basel, Switzerland, 3. – 7. September 2017. Nagia M., Gaid M., Behrends S., Beerhues L., Novel PPAP-related prenyltransferases, 4. SynFoBiA -Kolloquium des Pharmaverfahrenstechnik (PVZ), Braunschweig, Germany, 26. February 2016. Nagia M., Gaid M., Beurele T., Biedermann E., Beerhues L., Aromatic Prenyltransferases from Hypericum sampsonii, Postgraduate workshop of the section „Natural Products“ German Society for Plant Sciences (DBG), Meisdorf, Germany , 11.
  • Hypericaceae) Heritiana S

    Hypericaceae) Heritiana S

    University of Missouri, St. Louis IRL @ UMSL Dissertations UMSL Graduate Works 5-19-2017 Systematics, Biogeography, and Species Delimitation of the Malagasy Psorospermum (Hypericaceae) Heritiana S. Ranarivelo University of Missouri-St.Louis, [email protected] Follow this and additional works at: https://irl.umsl.edu/dissertation Part of the Botany Commons Recommended Citation Ranarivelo, Heritiana S., "Systematics, Biogeography, and Species Delimitation of the Malagasy Psorospermum (Hypericaceae)" (2017). Dissertations. 690. https://irl.umsl.edu/dissertation/690 This Dissertation is brought to you for free and open access by the UMSL Graduate Works at IRL @ UMSL. It has been accepted for inclusion in Dissertations by an authorized administrator of IRL @ UMSL. For more information, please contact [email protected]. Systematics, Biogeography, and Species Delimitation of the Malagasy Psorospermum (Hypericaceae) Heritiana S. Ranarivelo MS, Biology, San Francisco State University, 2010 A Dissertation Submitted to The Graduate School at the University of Missouri-St. Louis in partial fulfillment of the requirements for the degree Doctor of Philosophy in Biology with an emphasis in Ecology, Evolution, and Systematics August 2017 Advisory Committee Peter F. Stevens, Ph.D. Chairperson Peter C. Hoch, Ph.D. Elizabeth A. Kellogg, PhD Brad R. Ruhfel, PhD Copyright, Heritiana S. Ranarivelo, 2017 1 ABSTRACT Psorospermum belongs to the tribe Vismieae (Hypericaceae). Morphologically, Psorospermum is very similar to Harungana, which also belongs to Vismieae along with another genus, Vismia. Interestingly, Harungana occurs in both Madagascar and mainland Africa, as does Psorospermum; Vismia occurs in both Africa and the New World. However, the phylogeny of the tribe and the relationship between the three genera are uncertain.
  • Assessment Report on Hypericum Perforatum L., Herba

    Assessment Report on Hypericum Perforatum L., Herba

    European Medicines Agency Evaluation of Medicines for Human Use London, 12 November 2009 Doc. Ref.: EMA/HMPC/101303/2008 COMMITTEE ON HERBAL MEDICINAL PRODUCTS (HMPC) ASSESSMENT REPORT ON HYPERICUM PERFORATUM L., HERBA 7 Westferry Circus, Canary Wharf, London, E14 4HB, UK Tel. (44-20) 74 18 84 00 Fax (44-20) 75 23 70 51 E-mail: [email protected] http://www.emea.europa.eu © European Medicines Agency, 2009. Reproduction is authorised provided the source is acknowledged TABLE OF CONTENTS I. REGULATORY STATUS OVERVIEW...................................................................................4 II. ASSESSMENT REPORT............................................................................................................5 II.1 INTRODUCTION..........................................................................................................................6 II.1.1 Description of the herbal substance(s), herbal preparation(s) or combinations thereof 6 II.1.1.1 Herbal substance:........................................................................................................ 6 II.1.1.2 Herbal preparation(s): ................................................................................................ 7 II.1.1.3 Combinations of herbal substance(s) and/or herbal preparation(s)........................... 9 Not applicable. ................................................................................................................................9 II.1.1.4 Vitamin(s) ...................................................................................................................
  • Dr. Duke's Phytochemical and Ethnobotanical Databases List of Chemicals for Kidney Stones

    Dr. Duke's Phytochemical and Ethnobotanical Databases List of Chemicals for Kidney Stones

    Dr.
  • Toxicity Assessment of Hypericum Olympicum Subsp. Olympicum L. On

    Toxicity Assessment of Hypericum Olympicum Subsp. Olympicum L. On

    J Appl Biomed journal homepage: http://jab.zsf.jcu.cz DOI: 10.32725/jab.2020.002 Journal of Applied Biomedicine Original research article Toxicity assessment of Hypericum olympicum subsp. olympicum L. on human lymphocytes and breast cancer cell lines Necmiye Balikci 1, Mehmet Sarimahmut 1, Ferda Ari 1, Nazlihan Aztopal 1, 2, Mustafa Zafer Özel 3, Engin Ulukaya 1, 4, Serap Celikler 1 * 1 Uludag University, Faculty of Science and Arts, Department of Biology, Bursa, Turkey 2 Istinye University, Faculty of Science and Literature, Department of Molecular Biology and Genetics, Istanbul, Turkey 3 University of York, Department of Chemistry, Heslington, York, United Kingdom 4 Istinye University, Faculty of Medicine, Department of Medical Biochemistry, Istanbul, Turkey Abstract There is a limited number of studies about the constituents ofHypericum olympicum subsp. olympicum and its genotoxic and cytotoxic potency. We examined the possible antigenotoxic/genotoxic properties of methanolic extract of H. olympicum subsp. olympicum (HOE) on human lymphocytes by employing sister chromatid exchange, micronucleus and comet assay and analyzed its chemical composition by GCxGC-TOF/MS. The anti-growth activity against MCF-7 and MDA-MB-231 cell lines was assessed by using the ATP viability assay. Cell death mode was investigated with fluorescence staining and ELISA assays. The major components of the flower and trunk were determined as eicosane, heptacosane, 2-propen-1-ol, hexahydrofarnesyl acetone and α-muurolene. HOE caused significant DNA damage at selected doses (250–750 µg/ml) while chromosomal damage was observed at higher concentrations (500 and 750 µg/ml). HOE demonstrated anti-growth activity in a dose-dependent manner between 3.13–100 µg/ml.