PAK1 Antibody Cat. No.: 63-291

PAK1 Antibody

Confocal immunofluorescent analysis of PAK1 Antibody Formalin-fixed and paraffin-embedded human colon (T423) with Hela cell followed by Alexa Fluor 488- carcinoma tissue reacted with PAK1 Antibody (T423) , conjugated goat anti-rabbit lgG (green). Actin filaments which was peroxidase-conjugated to the secondary have been labeled with Alexa Fluor 555 phalloidin (red). antibody, followed by DAB staining.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat, Rabbit, HOMOLOGY: Xenopus

This PAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 401-430 amino acids from human PAK1.

TESTED APPLICATIONS: IF, IHC-P, WB

September 30, 2021 1 https://www.prosci-inc.com/pak1-antibody-63-291.html For WB starting dilution is: 1:1000

APPLICATIONS: For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50

PREDICTED MOLECULAR 61 kDa WEIGHT:

Properties

This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification.

CLONALITY: Polyclonal

ISOTYPE: Rabbit Ig

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.

CONCENTRATION: batch dependent

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: PAK1

Serine/threonine-protein kinase PAK 1, Alpha-PAK, p21-activated kinase 1, PAK-1, p65- ALTERNATE NAMES: PAK, PAK1

ACCESSION NO.: Q13153

PROTEIN GI NO.: 90111767

GENE ID: 5058

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

September 30, 2021 2 https://www.prosci-inc.com/pak1-antibody-63-291.html PAK1, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It is likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. The protein interacts tightly with GTP-bound but not BACKGROUND: GDP-bound CDC42/P21 and RAC1. PAK1 binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. It is a component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. The protein is autophosphorylated when activated by CDC42/p21. Structurally, the PAK1 contains 1 CRIB domain.

REFERENCES: 1) Chen, S., et al., J. Biol. Chem. 278(22):20029-20036 (2003).

2) Sells, M.A., et al., Curr. Biol. 7(3):202-210 (1997).

3) Brown, J.L., et al., Curr. Biol. 6(5):598-605 (1996).

ANTIBODIES FOR RESEARCH USE ONLY.

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