DOCSLIB.ORG

Chlorella Vulgaris) and a Cyanobacterium (Chrysosporum Ovalisporum)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Chlorella Vulgaris) and a Cyanobacterium (Chrysosporum Ovalisporum) https://doi.org/10.15454/BGEP-SC64 Bulletin de veille du réseau d'écotoxicologie terrestre et aquatique N°45, Juin 2020 Réalisé par l’équipe de veille sur la période du 1er mai au 30 juin 2020. Colette Bertrand, Christian Mougin (UMR 1402 EcoSys), Annette Berard, Céline Pélosi (UMR 1114 EMMAH), Soizic Morin (UR 1454 EABX), Olivier Crouzet (UPFS – OFB) et Pascale Karmasyn-Veyrines (DipSO) Destinataires : les membres de la liste : [email protected] Edito Voici notre 45ème bulletin de veille, toujours dense ! Nous remercions les nombreux lecteurs qui ont répondu à notre enquête portant sur votre percpetion du bulletin. Nous vous présenterons prochainement les résultats ainsi que nos propositions d’évolution. Dans ce bulletin, nous amorçons une thématique « biocontôle » concernant l’impact des molécules d’origine biologique. Nous vous proposons dans ce bulletin une tribune portant sur la bioaccumulation et le transfert trophique de contaminants dans les écosystèmes aquatiques : challenges & perspectives pour le réseau Ecotox. Le texte est également disponible sous forme de fiche thématique en téléchargement sur notre site ECOTOX : https://www6.inrae.fr/ecotox/Productions/Fiches-thematiques/Fiche-thematique-N-27-juin-2020 N’oubliez pas de nous transmettre les informations que vous souhaitez diffuser, notamment vos publications que nous pourrions avoir oubliées. L’équipe vous souhaite un bel été et une bonne lecture de ce bulletin ! Contact : [email protected] 1 https://doi.org/10.15454/BGEP-SC64 SOMMAIRE TRIBUNE LIBRE .......................................................................................................................... 12 ERA / PUBLICATIONS SCIENTIFIQUES / COMMUNAUTES MICROBIENNES AQUATIQUES. 16 Ecotoxicological effects of sulfonamides and fluoroquinolones and their removal by a green alga (Chlorella vulgaris) and a cyanobacterium (Chrysosporum ovalisporum) .................................................................................................16 Microplastics provide new microbial niches in aquatic environments .......................................................................16 Behavior of tetracycline and polystyrene nanoparticles in estuaries and their joint toxicity on marine microalgae Skeletonema costatum ..............................................................................................................................................17 Combined effects of 17 beta-estradiol and copper on growth, biochemical characteristics and pollutant removals of freshwater microalgae Scenedesmus dimorphus .......................................................................................................17 Pesticide Mixtures Cause Short-Term, Reversible Effects on the Function of Autotrophic Periphyton Assemblages .17 Selective grazing behaviour of chironomids on microalgae under pesticide pressure ................................................18 Effects of Combined Ag and ZnO Nanoparticles on Microbial Communities from Crab Orchard Creek, Illinois, USA ..18 Chronic exposure to CuO nanoparticles induced community structure shift and a delay inhibition of microbial functions in multi-species biofilms ............................................................................................................................19 Microplastics affect sedimentary microbial communities and nitrogen cycling .........................................................20 Microalgal Metallothioneins and Phytochelatins and Their Potential Use in Bioremediation ....................................20 Nanoplastics exposure modulate lipid and pigment compositions in diatoms ...........................................................20 Impact of polystyrene nanoparticles on marine diatom Skeletonema marinoi chain assemblages and consequences on their ecological role in marine ecosystems ...........................................................................................................21 Interplay between extracellular polymeric substances (EPS) from a marine diatom and model nanoplastic through eco-corona formation ................................................................................................................................................21 An exploratory study of benthic diatom communities in stormwater ponds of different land uses and varying biocide contamination ...............................................................................................................................................22 Differential Effects of the Allelochemical Juglone on Growth of Harmful and Non-Target Freshwater Algae ............22 Influence of biofilm on the transport and deposition behaviors of nano- and micro-plastic particles in quartz sand.23 Pesticide bioaccumulation in epilithic biofilms as a biomarker of agricultural activities in a representative watershed ..................................................................................................................................................................................23 Investigating the composition and distribution of microplastics surface biofilms in coral areas ................................24 Do environmental concentrations of zinc oxide nanoparticle pose ecotoxicological risk to aquatic fungi associated with leaf litter decomposition? ..................................................................................................................................24 Toxicity of ten herbicides to the tropical marine microalgae Rhodomonas salina .....................................................25 Effect of norfloxacin on performance, microbial enzymatic activity and microbial community of a sequencing batch reactor .......................................................................................................................................................................25 Dissolved organic matter does not promote glyphosate degradation in auto-heterotrophic aquatic microbial communities ..............................................................................................................................................................26 Impacts of organic matter on the toxicity of biosynthesized silver nanoparticles to green microalgae Chlorella vulgaris ......................................................................................................................................................................26 Biofilms Provide New Insight into Pesticide Occurrence in Streams and Links to Aquatic Ecological Communities ....26 Infrared spectroscopy as a tool to monitor interactions between nanoplastics and microalgae ................................27 Effects of Bisphenol A on the microalga Chlamydomonas reinhardtii and the clam Corbicula fluminea ....................27 Evaluation of toxic effects of platinum-based antineoplastic drugs (cisplatin, carboplatin and oxaliplatin) on green alga Chlorella vulgaris ...............................................................................................................................................28 Major Role of Surrounding Environment in Shaping Biofilm Community Composition on Marine Plastic Debris .......28 Lanthanum and Cerium Toxicity to the Freshwater Green Alga Chlorella fusca: Applicability of the Biotic Ligand Model ........................................................................................................................................................................29 Cultivation substrata differentiate the properties of river biofilm EPS and their binding of heavy metals: A spectroscopic insight .................................................................................................................................................29 Reproductive cycle progression arrest and modification of cell morphology (shape and biovolume) in the alga Pseudokirchneriella subcapitata exposed to metolachlor ..........................................................................................30 2 https://doi.org/10.15454/BGEP-SC64 Resistant fungi isolated from contaminated uranium mine in Brazil shows a high capacity to uptake uranium from water .........................................................................................................................................................................30 Understanding the influence of glyphosate on the structure and function of freshwater microbial community in a microcosm .................................................................................................................................................................31 Species-specific sensitivity of three microalgae to sediment elutriates .....................................................................31 Effects of oxytetracycline on growth and chlorophyll a fluorescence in green algae (Chlorella vulgaris), diatom (Phaeodactylum tricornutum) and cyanobacteria (Microcystis aeruginosa and Nodularia spumigena) .....................31 Ecotoxicological effects of alpha-cypermethrin on freshwater alga Chlorella sp.: Growth inhibition and oxidative stress studies .............................................................................................................................................................32 The potential assessment of green alga Chlamydomonas reinhardtii CC-503 in the biodegradation of benz(a)anthracene and the related mechanism analysis ...........................................................................................32 Nanoplastics
Load more

Recommended publications
  • Product Sheet Info
    Product Information Sheet for HM-331 Sporosarcina sp., Strain 2681 Incubation: Temperature: 37°C Atmosphere: Aerobic Catalog No. HM-331 Propagation: 1. Keep vial frozen until ready for use, then thaw. For research use only. Not for human use. 2. Transfer the entire thawed aliquot into a single tube of broth. Contributor: 3. Use several drops of the suspension to inoculate an Kimberlee A. Musser, Ph.D., Chief, Bacterial Diseases, agar slant and/or plate. Division of Infectious Diseases, Wadsworth Center, New York 4. Incubate the tubes and plate at 37°C for 48 hours. State Department of Health, Albany, New York Citation: Manufacturer: NIH Biodefense and Emerging Infections Acknowledgment for publications should read “The following reagent was obtained through the NIH Biodefense and Research Resource Repository Emerging Infections Research Resources Repository, NIAID, NIH as part of the Human Microbiome Project: Sporosarcina Product Description: sp., Strain 2681, HM-331.” Bacteria Classification: Planococcaceae, Sporosarcina Species: Sporosarcina sp. Biosafety Level: 2 Strain: 2681 Original Source: Sporosarcina sp., strain 2681 was isolated Appropriate safety procedures should always be used with 1 this material. Laboratory safety is discussed in the following from human blood. Comments: Sporosarcina sp., strain 2681 is a reference publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and genome for The Human Microbiome Project (HMP). HMP Prevention, and National Institutes of Health. Biosafety in is an initiative to identify and characterize human microbial flora. The complete genome of Sporosarcina sp., strain Microbiological and Biomedical Laboratories. 5th ed. Washington, DC: U.S. Government Printing Office, 2007; see 2681 is currently being sequenced at the Human Genome www.cdc.gov/od/ohs/biosfty/bmbl5/bmbl5toc.htm.
    [Show full text]
  • Original Article Bioremediation of Hexavalent Chromium Pollution By
    Biomed Environ Sci, 2016; 29(2): 127-136 127 Original Article Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China* ZHAO Ran1,2,#, WANG Bi1, CAI Qing Tao3, LI Xiao Xia1, LIU Min1, HU Dong1, GUO Dong Bei1,2, WANG Juan4, and FAN Chun1,2,# 1. School of Public Health, Xiamen University, Xiamen 361102, Fujian, China; 2. State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Xiamen 361102, Fujian, China; 3. Shanghai Jinshan District Center for Disease Control and Prevention, Shanghai 201599, China; 4. Department of Preventive Medicine, Xiamen Medical School, Xiamen 361008, Fujian, China Abstract Objective Cr(VI) removal from industrial effluents and sediments has attracted the attention of environmental researchers. In the present study, we aimed to isolate bacteria for Cr(VI) bioremediation from sediment samples and to optimize parameters of biodegradation. Methods Strains with the ability to tolerate Cr(VI) were obtained by serial dilution and spread plate methods and characterized by morphology, 16S rDNA identification, and phylogenetic analysis. Cr(VI) was determined using the 1,5-diphenylcarbazide method, and the optimum pH and temperature for degradation were studied using a multiple-factor mixed experimental design. Statistical analysis methods were used to analyze the results. Results Fifty-five strains were obtained, and one strain (Sporosarcina saromensis M52; patent application number: 201410819443.3) having the ability to tolerate 500 mg Cr(VI)/L was selected to optimize the degradation conditions. M52 was found be able to efficiently remove 50-200 mg Cr(VI)/L in 24 h, achieving the highest removal efficiency at pH 7.0-8.5 and 35 °C.
    [Show full text]
  • Manganese-II Oxidation and Copper-II Resistance in Endospore Forming Firmicutes Isolated from Uncontaminated Environmental Sites
    AIMS Environmental Science, 3(2): 220-238. DOI: 10.3934/environsci.2016.2.220 Received 21 January 2016, Accepted 05 April 2016, Published 12 April 2016 http://www.aimspress.com/journal/environmental Research article Manganese-II oxidation and Copper-II resistance in endospore forming Firmicutes isolated from uncontaminated environmental sites Ganesan Sathiyanarayanan 1,†, Sevasti Filippidou 1,†, Thomas Junier 1,2, Patricio Muñoz Rufatt 1, Nicole Jeanneret 1, Tina Wunderlin 1, Nathalie Sieber 1, Cristina Dorador 3 and Pilar Junier 1,* 1 Laboratory of Microbiology, Institute of Biology, University of Neuchâtel, Emile-Argand 11, CH-2000 Neuchâtel, Switzerland 2 Vital-IT group, Swiss Institute of Bioinformatics, CH-1000 Lausanne, Switzerland 3 Laboratorio de Complejidad Microbiana y Ecología Funcional; Departamento de Biotecnología; Facultad de Ciencias del Mar y Recursos Biológicos, Universidad de Antofagasta; CL-, 1270190, Antofagasta, Chile * Correspondence: Email: [email protected]; Tel: +41-32-718-2244; Fax: +41-32-718-3001. † These authors contributed equally to this work. Abstract: The accumulation of metals in natural environments is a growing concern of modern societies since they constitute persistent, non-degradable contaminants. Microorganisms are involved in redox processes and participate to the biogeochemical cycling of metals. Some endospore-forming Firmicutes (EFF) are known to oxidize and reduce specific metals and have been isolated from metal-contaminated sites. However, whether EFF isolated from uncontaminated sites have the same capabilities has not been thoroughly studied. In this study, we measured manganese oxidation and copper resistance of aerobic EFF from uncontaminated sites. For the purposes of this study we have sampled 22 natural habitats and isolated 109 EFF strains.
    [Show full text]
  • Appendix 1. Validly Published Names, Conserved and Rejected Names, And
    Appendix 1. Validly published names, conserved and rejected names, and taxonomic opinions cited in the International Journal of Systematic and Evolutionary Microbiology since publication of Volume 2 of the Second Edition of the Systematics* JEAN P. EUZÉBY New phyla Alteromonadales Bowman and McMeekin 2005, 2235VP – Valid publication: Validation List no. 106 – Effective publication: Names above the rank of class are not covered by the Rules of Bowman and McMeekin (2005) the Bacteriological Code (1990 Revision), and the names of phyla are not to be regarded as having been validly published. These Anaerolineales Yamada et al. 2006, 1338VP names are listed for completeness. Bdellovibrionales Garrity et al. 2006, 1VP – Valid publication: Lentisphaerae Cho et al. 2004 – Valid publication: Validation List Validation List no. 107 – Effective publication: Garrity et al. no. 98 – Effective publication: J.C. Cho et al. (2004) (2005xxxvi) Proteobacteria Garrity et al. 2005 – Valid publication: Validation Burkholderiales Garrity et al. 2006, 1VP – Valid publication: Vali- List no. 106 – Effective publication: Garrity et al. (2005i) dation List no. 107 – Effective publication: Garrity et al. (2005xxiii) New classes Caldilineales Yamada et al. 2006, 1339VP VP Alphaproteobacteria Garrity et al. 2006, 1 – Valid publication: Campylobacterales Garrity et al. 2006, 1VP – Valid publication: Validation List no. 107 – Effective publication: Garrity et al. Validation List no. 107 – Effective publication: Garrity et al. (2005xv) (2005xxxixi) VP Anaerolineae Yamada et al. 2006, 1336 Cardiobacteriales Garrity et al. 2005, 2235VP – Valid publica- Betaproteobacteria Garrity et al. 2006, 1VP – Valid publication: tion: Validation List no. 106 – Effective publication: Garrity Validation List no. 107 – Effective publication: Garrity et al.
    [Show full text]
  • Biological Influence of Cry1ab Gene Insertion on the Endophytic Bacteria Community in Transgenic Rice
    Turkish Journal of Biology Turk J Biol (2018) 42: 231-239 http://journals.tubitak.gov.tr/biology/ © TÜBİTAK Research Article doi:10.3906/biy-1708-32 Biological influence of cry1Ab gene insertion on the endophytic bacteria community in transgenic rice 1 1 1,2, Xu WANG , Mengyu CAI , Yu ZHOU * 1 State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science Technology, Anhui Agricultural University, Heifei, P.R. China 2 State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control, Agricultural Ministry Key Laboratory for Pesticide Residue Detection, Zhejiang Province Key Laboratory for Food Safety, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, P.R. China Received: 13.08.2017 Accepted/Published Online: 19.04.2018 Final Version: 13.06.2018 Abstract: The commercial release of genetically modified (GMO) rice for insect control in China is a subject of debate. Although a series of studies have focused on the safety evaluation of the agroecosystem, the endophytes of transgenic rice are rarely considered. Here, the influence of endophyte populations and communities was investigated and compared for transgenic and nontransgenic rice. Population-level investigation suggested that cry1Ab gene insertion influenced to a varying degree the rice endophytes at the seedling stage, but a significant difference was only observed in leaves of Bt22 (Zhejiang22 transgenic rice) between the GMO and wild-type rice. Community-level analysis using the 16S rRNA gene showed that strains of the phyla Proteobacteria and Firmicutes were the predominant groups occurring in the three transgenic rice plants and their corresponding parents.
    [Show full text]
  • Sporosarcina Luteola Sp. Nov. Isolated from Soy Sauce Production Equipment in Japan
    J. Gen. Appl. Microbiol., 55, 217‒223 (2009) Full Paper Sporosarcina luteola sp. nov. isolated from soy sauce production equipment in Japan Tatsuya Tominaga,1,* Sun-Young An,2 Hiroshi Oyaizu,3 and Akira Yokota2 1 Saitama Industrial Technology Center North Institute, Kumagaya, Saitama 360‒0031, Japan 2 Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo 113‒0032, Japan 3 Biotechnology Research Center, The University of Tokyo, Bunkyo-ku, Tokyo 113‒8657, Japan (Received January 19, 2009; Accepted March 6, 2009) A Gram-variable, spore-forming, motile rod, designated strain Y1T, was isolated from the hopper surface of equipment used for soy sauce production. Phylogenetic analysis based on 16S rRNA gene sequence revealed that Y1T is affi liated phylogenetically to the genus Sporosarcina, and the strain showed sequence similarities of 95.8‒99.2% to those of Sporosarcina species with validly published names. The values of DNA-DNA relatedness between strain Y1T and related type strains of the genus Sporosarcina were below 27%. The major cellular fatty acids were iso- C15:0 and anteiso-C15:0. The cell-wall peptidoglycan was of the A4α type (Lys-Glu) and the major isoprenoid quinone was MK-7. The major polar lipids were diphosphatidylglycerol, phosphatidyl- glycerol, and phosphatidylethanolamine. The genomic DNA G+C content of the strain was 43.6 mol%. On the basis of phylogenetic analysis and physiological and chemotaxonomic data, the isolate represents a novel species of the genus Sporosarcina, for which the name Sporo- sarcina luteola sp. nov. is proposed. The type strain is strain Y1T (=JCM 15791T=NRRL B-59180T =NBRC 105378T=CIP 109917T=NCIMB 14541T).
    [Show full text]
  • Evaluation of FISH for Blood Cultures Under Diagnostic Real-Life Conditions
    Original Research Paper Evaluation of FISH for Blood Cultures under Diagnostic Real-Life Conditions Annalena Reitz1, Sven Poppert2,3, Melanie Rieker4 and Hagen Frickmann5,6* 1University Hospital of the Goethe University, Frankfurt/Main, Germany 2Swiss Tropical and Public Health Institute, Basel, Switzerland 3Faculty of Medicine, University Basel, Basel, Switzerland 4MVZ Humangenetik Ulm, Ulm, Germany 5Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, Hamburg, Germany 6Institute for Medical Microbiology, Virology and Hygiene, University Hospital Rostock, Rostock, Germany Received: 04 September 2018; accepted: 18 September 2018 Background: The study assessed a spectrum of previously published in-house fluorescence in-situ hybridization (FISH) probes in a combined approach regarding their diagnostic performance with incubated blood culture materials. Methods: Within a two-year interval, positive blood culture materials were assessed with Gram and FISH staining. Previously described and new FISH probes were combined to panels for Gram-positive cocci in grape-like clusters and in chains, as well as for Gram-negative rod-shaped bacteria. Covered pathogens comprised Staphylococcus spp., such as S. aureus, Micrococcus spp., Enterococcus spp., including E. faecium, E. faecalis, and E. gallinarum, Streptococcus spp., like S. pyogenes, S. agalactiae, and S. pneumoniae, Enterobacteriaceae, such as Escherichia coli, Klebsiella pneumoniae and Salmonella spp., Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Bacteroides spp. Results: A total of 955 blood culture materials were assessed with FISH. In 21 (2.2%) instances, FISH reaction led to non-interpretable results. With few exemptions, the tested FISH probes showed acceptable test characteristics even in the routine setting, with a sensitivity ranging from 28.6% (Bacteroides spp.) to 100% (6 probes) and a spec- ificity of >95% in all instances.
    [Show full text]
  • Domibacillus Tundrae Sp
    The 21st International Symposium on Polar Science May 19-20, 2015 Domibacillus tundrae sp. nov., isolated from active layer soil of tussock tundra in Council, Alaska Hye Ryeon Gyeong, Kiwoon Baek, Chung Yeon Hwang, Key Hun Park, Hye Min Kim, HongKum Lee and Yoo Kyung Lee* E-mail to [email protected] Korea Polar Research Institute, Republic of Korea [email protected] ABSTRACT A novel Gram-positive, spore-forming, aerobic, motile and rod-shaped bacterium designated as strain PAMC 80007T was isolated from active layer soil sample of moist acidic tundra in Council, Alaska. Optimal growth of strain PAMC 80007T was observed at 30 ºC, pH 7.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain PAMC 80007T belonged to the genus Domibacillus. This strain was closely related to Domibacillus enclensis (98.3 %), D. robiginosus (98.3 %) and D. indicus (97.2 %). Genomic DNA G+C content was 43.5 mol % and genomic relatedness analyses based on the average nucleotide identity and the genome-to-genome distance showed that strain PAMC 80007T is clearly distinguished from the closely related Domibacillus species. The major fatty acids (>5 %) were iso-C15:0 (24.7 %), C16:1 ω11c (16.8 %), anteiso-C15:0 (16.5 %), C16:0 (15.6 %) and anteiso-C17:0 (8.7 %). The major respiratory isoprenoid quinines MK-6 and MK-7, and the polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, phospholipid and two unidentified lipids. The major whole-cell sugar was ribose with minor quantity of glucose and meso-diaminopimelic acid (type A1γ) was present in the cell-wall peptidoglycan.
    [Show full text]
  • Exploring Polar Microbiomes As Source of Bioactive Molecules
    Exploring Polar Microbiomes as Source of Bioactive Molecules Adriana Isabel Correia Rego Mestrado em Biologia Celular e Molecular Departamento de Biologia da Faculdade de Ciências da Universidade do Porto Dissertação de Mestrado 2016/2017 Orientador: Pedro Leão, Investigador FCT, Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR) Co-orientador: Catarina Magalhães, Investigador FCT, Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR) e Professora Auxiliar Convidada FCUP FCUP ii Exploring Polar Microbiomes as Source of Bioactive Molecules Todas as correções determinadas pelo júri, e só essas, foram efetuadas. O Presidente do Júri, Porto, ______/______/_________ FCUP iii Exploring Polar Microbiomes as Source of Bioactive Molecules Agradecimentos Antes de mais, quero agradecer aos meus pais, sem o apoio dos quais nada disto se teria tornado possível. Agradecer aos meus orientadores, Pedro Leão e Catarina Magalhães, pela confiança depositada, por todos os conhecimentos partilhados e tempo despendido e pela oportunidade de fazer um trabalho de investigação desafiante e concretizador. Quero também agradecer à Teresa Martins, António Sousa e Inês Ribeiro, companheiros desta jornada, por toda a partilha de bons momentos e entreajuda. Em especial à Teresa pela amizade e ajuda incansável na química, ao António pela ajuda e paciência na análise bioinformática e à Inês, pela companhia e ajuda na realização dos ensaios. Agradecer à equipa do laboratório Ecobiotec, em especial à Fátima Carvalho e à Mafalda Baptista pela ajuda nos isolamentos bacterianos. Ao grupo de Bioinformática, à Maria Paola e ao António, por todos os ensinamentos e momentos de boa disposição. A toda a equipa do BBE, particularmente ao João, à Raquel e ao Vítor pelo fornecimento das estirpes da coleção de culturas e DNAs.
    [Show full text]
  • Universidade Federal Do Rio Grande Do Sul Instituto De Ciências Básicas Da Saúde Programa De Pós-Graduação Em Microbiologia Agrícola E Do Ambiente
    UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL INSTITUTO DE CIÊNCIAS BÁSICAS DA SAÚDE PROGRAMA DE PÓS-GRADUAÇÃO EM MICROBIOLOGIA AGRÍCOLA E DO AMBIENTE IDENTIFICAÇÃO FILOGENÉTICA E ATIVIDADE HIDROLÍTICA DE BACTÉRIAS ISOLADAS DE ÁGUAS DA REGIÃO DA ELEVAÇÃO DO RIO GRANDE E SEDIMENTOS DO LESTE DO ATLÂNTICO SUL Marcus Adonai Castro da Silva PORTO ALEGRE, RS, BRASIL JUNHO, 2013 UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL INSTITUTO DE CIÊNCIAS BÁSICAS DA SAÚDE PROGRAMA DE PÓS-GRADUAÇÃO EM MICROBIOLOGIA AGRÍCOLA E DO AMBIENTE IDENTIFICAÇÃO FILOGENÉTICA E ATIVIDADE HIDROLÍTICA DE BACTÉRIAS ISOLADAS DE ÁGUAS DA REGIÃO DA ELEVAÇÃO DO RIO GRANDE E SEDIMENTOS DO LESTE DO ATLÂNTICO SUL Marcus Adonai Castro da Silva Oceanógrafo (UNIVALI) Mestre em Ciência e Tecnologia Ambiental (UNIVALI) Tese apresentada como requisito parcial para a obtenção do grau de Doutor em Microbiologia Agrícola e do Ambiente na área de Microbiologia do Ambiente PORTO ALEGRE, RS, BRASIL JUNHO, 2013 ii AGRADECIMENTOS Aos professores Dr. Gertrudes Corção e André Oliveira de Souza Lima pela orientação, ensinamentos e apoio em vários aspectos durante a realização deste trabalho. Ao professor Dr. José Angel Alvarez Perez, coordenador do projeto “Patterns and Processes of the Ecosystems of the Southern Mid-Atlantic” – Census of Marine Life (CoML), pelo suporte no embarques e coleta de amostras. Ao Dr. André Silva Barreto e aos biólogos Estácio Jussie Odisi e Felipe Araújo Teixeira Paulo pela coleta de amostras. Aos pesquisadores Angélica Cavalett, Ananda Spinner, Daniela Cristina Rosa, Regina Beltrame Jasper, Maria Carolina Quecine, Maria Letícia Bonatelli, Aline Pizzirani-Kleiner, Aline Diniz, Rodrigo Penque, Maria Letícia Biavatti, Jurandir Pereira Filho, e Leonardo Rübi Rorig pelas suas respectivas contribuições ao trabalho.
    [Show full text]
  • Chungangia Koreensis Gen. Nov., Sp. Nov., Isolated from Marine Sediment
    International Journal of Systematic and Evolutionary Microbiology (2012), 62, 1914–1920 DOI 10.1099/ijs.0.028837-0 Chungangia koreensis gen. nov., sp. nov., isolated from marine sediment Wonyong Kim,1 Jitsopin Traiwan,1 Mi-Hak Park,1 Min Young Jung,1 Su-Jin Oh,1 Jung-Hoon Yoon2 and Ampaitip Sukhoom3 Correspondence 1Department of Microbiology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Wonyong Kim Dongjak-ku, Seoul 156-756, Republic of Korea [email protected] 2School of Life Science and Biotechnology, Sungkyunkwan University, 300 Cheoncheon-dong, Jangan-gu, Suwon, Gyeonggi-do 440-746, Republic of Korea 3Department of Microbiology, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand A Gram-staining-positive, strictly aerobic, non-spore-forming, rod-shaped bacterial strain, CAU 9163T, was isolated from marine sediment collected in the Republic of Korea and its taxonomic position was investigated using a polyphasic approach. The novel strain grew optimally at 30 6C and pH 8.0. In phylogenetic analysis based on 16S rRNA gene sequences, strain CAU 9163T formed a hitherto unknown lineage within the order Bacillales, which contains the genera Planomicrobium, Planococcus, Sporosarcina, Rummeliibacillus, Viridibacillus, Lysinibacillus and Bacillus. The levels of 16S rRNA gene sequence similarity between the novel strain and any established bacterial species were all ,95.7 %. The major isoprenoid quinines of strain CAU 9163T were MK-8 (65.2 %) and MK-7 (22.8 %) and the predominant fatty acid was anteiso- C15 : 0. The peptidoglycan was of the A4a type and based on L-Lys-D-Asp. The major whole-cell sugars were ribose and glucose.
    [Show full text]
  • Chryseomicrobium Excrementi Sp. Nov., a Gram-Stain-Positive Rod-Shaped Bacterium Isolated from an Earthworm (Eisenia Fetida) Cast
    TAXONOMIC DESCRIPTION Saha et al., Int J Syst Evol Microbiol 2018;68:2165–2171 DOI 10.1099/ijsem.0.002791 Chryseomicrobium excrementi sp. nov., a Gram-stain-positive rod-shaped bacterium isolated from an earthworm (Eisenia fetida) cast Tilak Saha,1,2 Biswanath Chakraborty,3 Sayak Das,4 Nagendra Thakur4 and Ranadhir Chakraborty1,* Abstract A Gram-stain-positive, rod-shaped, slightly halotolerant, nitrate-reducing bacterial strain, designated ET03T, was isolated from the cast of an earthworm (Eisenia fetida) reared at the Centre of Floriculture and Agribusiness Management, University of North Bengal at Siliguri, West Bengal, India. On the basis of 16S rRNA gene sequence phylogeny, the closest relative of strain ET03T was Chryseomicrobium palamuruense PU1T (99.1 % similarity). The DNA G+C content of strain ET03T was 42.9 mol%. Strain ET03T contained menaquinone-8 as the most predominant menaquinone and phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and phosphatidylglycerol as the main polar lipids. The diagnostic diamino acid was meso-diaminopimelic acid. Major cellular fatty acids were iso-C15 : 0,C16 : 1!7c alcohol and iso-C16 : 0. Other biochemical and physiological analyses supported genotypic and phenotypic differentiation of the strain ET03T from its nearest taxonomic neighbours: Chryseomicrobium palamuruense, Chryseomicrobium amylolyticum, Chryseomicrobium imtechense, Chryseomicrobium aureum and Chryseomicrobium deserti. The draft genome of strain ET03T consisted of 2.64 Mb distributed in 14 scaffolds (N50 894072). A total of 2728 genes were predicted and, of those, 2664 were protein-coding genes including genes involved in the degradation of polychlorinated biphenyl and several aromatic compounds. The isolate, therefore, represents a novel species, for which the name Chryseomicrobium excrementi sp.
    [Show full text]