Isolation of Carnobacterium Piscicola and an on Corh Ynch Us M Ykiss
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Cortisol-Related Signatures of Stress in the Fish Microbiome
fmicb-11-01621 July 11, 2020 Time: 15:28 # 1 ORIGINAL RESEARCH published: 14 July 2020 doi: 10.3389/fmicb.2020.01621 Cortisol-Related Signatures of Stress in the Fish Microbiome Tamsyn M. Uren Webster*, Deiene Rodriguez-Barreto, Sofia Consuegra and Carlos Garcia de Leaniz Centre for Sustainable Aquatic Research, College of Science, Swansea University, Swansea, United Kingdom Exposure to environmental stressors can compromise fish health and fitness. Little is known about how stress-induced microbiome disruption may contribute to these adverse health effects, including how cortisol influences fish microbial communities. We exposed juvenile Atlantic salmon to a mild confinement stressor for two weeks. We then measured cortisol in the plasma, skin-mucus, and feces, and characterized the skin and fecal microbiome. Fecal and skin cortisol concentrations increased in fish exposed to confinement stress, and were positively correlated with plasma cortisol. Elevated fecal cortisol was associated with pronounced changes in the diversity and Edited by: Malka Halpern, structure of the fecal microbiome. In particular, we identified a marked decline in the University of Haifa, Israel lactic acid bacteria Carnobacterium sp. and an increase in the abundance of operational Reviewed by: taxonomic units within the classes Clostridia and Gammaproteobacteria. In contrast, Heather Rose Jordan, cortisol concentrations in skin-mucus were lower than in the feces, and were not Mississippi State University, United States related to any detectable changes in the skin microbiome. Our results demonstrate that Timothy John Snelling, stressor-induced cortisol production is associated with disruption of the gut microbiome, Harper Adams University, United Kingdom which may, in turn, contribute to the adverse effects of stress on fish health. -
Comparative Genomic Analysis of Carnobacterium Maltaromaticum: Study of Diversity and Adaptation to Different Environments Christelle Iskandar
Comparative genomic analysis of Carnobacterium maltaromaticum: Study of diversity and adaptation to different environments Christelle Iskandar To cite this version: Christelle Iskandar. Comparative genomic analysis of Carnobacterium maltaromaticum: Study of diversity and adaptation to different environments. Food and Nutrition. Université de Lorraine, 2015. English. NNT : 2015LORR0245. tel-01754646 HAL Id: tel-01754646 https://hal.univ-lorraine.fr/tel-01754646 Submitted on 30 Mar 2018 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. AVERTISSEMENT Ce document est le fruit d'un long travail approuvé par le jury de soutenance et mis à disposition de l'ensemble de la communauté universitaire élargie. Il est soumis à la propriété intellectuelle de l'auteur. Ceci implique une obligation de citation et de référencement lors de l’utilisation de ce document. D'autre part, toute contrefaçon, plagiat, reproduction illicite encourt une poursuite pénale. Contact : [email protected] LIENS Code de la Propriété Intellectuelle. articles L 122. 4 -
Growth of Carnobacterium Spp. from Permafrost Under Low Pressure, Temperature, and Anoxic Atmosphere Has Implications for Earth Microbes on Mars
Growth of Carnobacterium spp. from permafrost under low pressure, temperature, and anoxic atmosphere has implications for Earth microbes on Mars Wayne L. Nicholsona,1, Kirill Krivushinb, David Gilichinskyb,2, and Andrew C. Schuergerc Departments of aMicrobiology and Cell Science and cPlant Pathology, Space Life Sciences Laboratory, University of Florida, Merritt Island, FL 32953; and bInstitute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences, Pushchino 142290 Moscow Region, Russian Federation Edited* by Henry J. Melosh, Purdue University, West Lafayette, IN, and approved November 9, 2012 (received for review June 8, 2012) The ability of terrestrial microorganisms to grow in the near-surface Results and Discussion environment of Mars is of importance to the search for life and Isolation of Microorganisms from Siberian Permafrost. Samples of protection of that planet from forward contamination by human permafrost obtained from the Siberian arctic (Fig. 1) were sus- and robotic exploration. Because most water on present-day Mars is pendedandplatedontrypticasesoybrothyeastextractsalt frozen in the regolith, permafrosts are considered to be terrestrial (TSBYS) medium and incubated at room temperature (ca. 23 °C) analogs of the martian subsurface environment. Six bacterial isolates for up to 28 d. Colonies were either picked or replica-plated onto were obtained from a permafrost borehole in northeastern Siberia fresh TSBYS plates and incubated for 30 d under low-PTA con- capable of growth under conditions of low temperature (0 °C), low ditions. Out of a total of ∼9.3 × 103 colonies tested from four pressure (7 mbar), and a CO2-enriched anoxic atmosphere. By 16S different permafrost soil samples, 6 colonies were observed to fi ribosomal DNA analysis, all six permafrost isolates were identi ed grow under low-PTA conditions (Table 1). -
New Insight Into Antimicrobial Compounds from Food and Marine-Sourced Carnobacterium Species Through Phenotype and Genome Analyses
microorganisms Article New Insight into Antimicrobial Compounds from Food and Marine-Sourced Carnobacterium Species through Phenotype and Genome Analyses Simon Begrem 1,2, Flora Ivaniuk 2, Frédérique Gigout-Chevalier 2, Laetitia Kolypczuk 2, Sandrine Bonnetot 2, Françoise Leroi 2, Olivier Grovel 1 , Christine Delbarre-Ladrat 2 and Delphine Passerini 2,* 1 University of Nantes, 44035 Nantes CEDEX 1, France; [email protected] (S.B.); [email protected] (O.G.) 2 IFREMER, BRM, EM3B Laboratory, 44300 Nantes CEDEX 3, France; fl[email protected] (F.I.); [email protected] (F.G.-C.); [email protected] (L.K.); [email protected] (S.B.); [email protected] (F.L.); [email protected] (C.D.-L.) * Correspondence: [email protected] Received: 6 July 2020; Accepted: 19 July 2020; Published: 21 July 2020 Abstract: Carnobacterium maltaromaticum and Carnobacterium divergens, isolated from food products, are lactic acid bacteria known to produce active and efficient bacteriocins. Other species, particularly those originating from marine sources, are less studied. The aim of the study is to select promising strains with antimicrobial potential by combining genomic and phenotypic approaches on large datasets comprising 12 Carnobacterium species. The biosynthetic gene cluster (BGCs) diversity of 39 publicly available Carnobacterium spp. genomes revealed 67 BGCs, distributed according to the species and ecological niches. From zero to six BGCs were predicted per strain and classified into four classes: terpene, NRPS (non-ribosomal peptide synthetase), NRPS-PKS (hybrid non-ribosomal peptide synthetase-polyketide synthase), RiPP (ribosomally synthesized and post-translationally modified peptide). In parallel, the antimicrobial activity of 260 strains from seafood products was evaluated. -
Growth of Carnobacterium Spp. from Permafrost Under Low Pressure, Temperature, and Anoxic Atmosphere Has Implications for Earth Microbes on Mars
Growth of Carnobacterium spp. from permafrost under low pressure, temperature, and anoxic atmosphere has implications for Earth microbes on Mars Wayne L. Nicholsona,1, Kirill Krivushinb, David Gilichinskyb,2, and Andrew C. Schuergerc Departments of aMicrobiology and Cell Science and cPlant Pathology, Space Life Sciences Laboratory, University of Florida, Merritt Island, FL 32953; and bInstitute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences, Pushchino 142290 Moscow Region, Russian Federation Edited* by Henry J. Melosh, Purdue University, West Lafayette, IN, and approved November 9, 2012 (received for review June 8, 2012) The ability of terrestrial microorganisms to grow in the near-surface Results and Discussion environment of Mars is of importance to the search for life and Isolation of Microorganisms from Siberian Permafrost. Samples of protection of that planet from forward contamination by human permafrost obtained from the Siberian arctic (Fig. 1) were sus- and robotic exploration. Because most water on present-day Mars is pendedandplatedontrypticasesoybrothyeastextractsalt frozen in the regolith, permafrosts are considered to be terrestrial (TSBYS) medium and incubated at room temperature (ca. 23 °C) analogs of the martian subsurface environment. Six bacterial isolates for up to 28 d. Colonies were either picked or replica-plated onto were obtained from a permafrost borehole in northeastern Siberia fresh TSBYS plates and incubated for 30 d under low-PTA con- capable of growth under conditions of low temperature (0 °C), low ditions. Out of a total of ∼9.3 × 103 colonies tested from four pressure (7 mbar), and a CO2-enriched anoxic atmosphere. By 16S different permafrost soil samples, 6 colonies were observed to fi ribosomal DNA analysis, all six permafrost isolates were identi ed grow under low-PTA conditions (Table 1). -
Characterization of Carnobacterium Species by Pyrolysis Mass Spectrometry
Journal of Applied Bacteriology 1995, 78, 8696 Characterization of Carnobacterium species by pyrolysis mass spectrometry L.N. Manchester, A. Toole and R. Goodacre Institute of Biological Sciences, University of Wales, A berystwyth , Dyfed, UK 5050/09/94: received 9 September 1994 and accepted 13 September 1994 L.N. MANCHESTER, A. TOOLE AND R. GOODACRE. 1995. Forty-eight strains of Carnobacterium were examined by pyrolysis mass spectrometry (PyMS). The effects of culture age and reproducibility over a 4 week period were also examined. The results were analysed by multivariate statistical techniques and compared with those from a previous numerical taxonomic study based on morphological, physiological and biochemical characteristics and with studies which used DNA-DNA and 16s rRNA sequence homologies. Taxonomic correlations were observed between the PyMS data and the previous studies. Culture age was observed to have little effect on the mass spectra obtained and the reproducibility study indicated that there was very little variation over the 4 week period. It was concluded that PyMS provides a reliable method for studying carnobacterial classification and provides a rapid way for clarifying and refining subgeneric relationships within the genus Carnobacterium. Further work may also show that it offers a potentially very rapid and accurate method for the identification of Carnobacterium. INTRODUCTION (ca 96-98%) and formed a group which is phylogenetically The genus Carnobacterium was proposed by Collins et al. distinct from other lactic acid bacteria. Vagococcus juvialis (1987) to describe the atypical lactobacilli poultry isolates of and the enterococci were observed to be the most closely Thornley and Sharpe (1959), Lactobacillus divergens related genera. -
Characterization of Bacterial Communities of Cold-Smoked Salmon During Storage
foods Article Characterization of Bacterial Communities of Cold-Smoked Salmon during Storage Aurélien Maillet 1,2,* , Pauline Denojean 2, Agnès Bouju-Albert 2 , Erwann Scaon 1 ,Sébastien Leuillet 1, Xavier Dousset 2, Emmanuel Jaffrès 2,Jérôme Combrisson 1 and Hervé Prévost 2 1 Biofortis Mérieux NutriSciences, 3 route de la Chatterie, 44800 Saint-Herblain, France; [email protected] (E.S.); [email protected] (S.L.); [email protected] (J.C.) 2 INRAE, UMR Secalim, Oniris, Route de Gachet, CS 44307 Nantes, France; [email protected] (P.D.); [email protected] (A.B.-A.); [email protected] (X.D.); [email protected] (E.J.); [email protected] (H.P.) * Correspondence: [email protected] Abstract: Cold-smoked salmon is a widely consumed ready-to-eat seafood product that is a fragile commodity with a long shelf-life. The microbial ecology of cold-smoked salmon during its shelf-life is well known. However, to our knowledge, no study on the microbial ecology of cold-smoked salmon using next-generation sequencing has yet been undertaken. In this study, cold-smoked salmon microbiotas were investigated using a polyphasic approach composed of cultivable methods, V3—V4 16S rRNA gene metabarcoding and chemical analyses. Forty-five cold-smoked salmon products processed in three different factories were analyzed. The metabarcoding approach highlighted 12 dominant genera previously reported as fish spoilers: Firmicutes Staphylococcus, Carnobacterium, Lactobacillus, β-Proteobacteria Photobacterium, Vibrio, Aliivibrio, Salinivibrio, Enterobacteriaceae Serratia, Citation: Maillet, A.; Denojean, P.; Pantoea γ Psychrobacter, Shewanella Pseudomonas Bouju-Albert, A.; Scaon, E.; Leuillet, , -Proteobacteria and . -
Bacteria Richness and Antibiotic-Resistance in Bats from a Protected Area in the Atlantic Forest of Southeastern Brazil
RESEARCH ARTICLE Bacteria richness and antibiotic-resistance in bats from a protected area in the Atlantic Forest of Southeastern Brazil VinõÂcius C. ClaÂudio1,2,3*, Irys Gonzalez2, Gedimar Barbosa1,2, Vlamir Rocha4, Ricardo Moratelli5, FabrõÂcio Rassy2 1 Centro de Ciências BioloÂgicas e da SauÂde, Universidade Federal de São Carlos, São Carlos, SP, Brazil, 2 FundacËão Parque ZooloÂgico de São Paulo, São Paulo, SP, Brazil, 3 Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil, 4 Centro de Ciências AgraÂrias, Universidade Federal de São Carlos, Araras, SP, Brazil, 5 Fiocruz Mata AtlaÃntica, FundacËão Oswaldo Cruz, Rio de Janeiro, RJ, a1111111111 Brazil a1111111111 [email protected] a1111111111 * a1111111111 a1111111111 Abstract Bats play key ecological roles, also hosting many zoonotic pathogens. Neotropical bat microbiota is still poorly known. We speculate that their dietary habits strongly influence OPEN ACCESS their microbiota richness and antibiotic-resistance patterns, which represent growing and Citation: ClaÂudio VC, Gonzalez I, Barbosa G, Rocha serious public health and environmental issue. Here we describe the aerobic microbiota V, Moratelli R, Rassy F (2018) Bacteria richness richness of bats from an Atlantic Forest remnant in Southeastern Brazil, and the antibiotic- and antibiotic-resistance in bats from a protected area in the Atlantic Forest of Southeastern Brazil. resistance patterns of bacteria of clinical importance. Oral and rectal cavities of 113 bats PLoS ONE 13(9): e0203411. https://doi.org/ from Carlos Botelho State Park were swabbed. Samples were plated on 5% sheep blood 10.1371/journal.pone.0203411 and MacConkey agar and identified by the MALDI-TOF technique. -
( 12 ) United States Patent
US009956282B2 (12 ) United States Patent ( 10 ) Patent No. : US 9 ,956 , 282 B2 Cook et al. (45 ) Date of Patent: May 1 , 2018 ( 54 ) BACTERIAL COMPOSITIONS AND (58 ) Field of Classification Search METHODS OF USE THEREOF FOR None TREATMENT OF IMMUNE SYSTEM See application file for complete search history . DISORDERS ( 56 ) References Cited (71 ) Applicant : Seres Therapeutics , Inc. , Cambridge , U . S . PATENT DOCUMENTS MA (US ) 3 ,009 , 864 A 11 / 1961 Gordon - Aldterton et al . 3 , 228 , 838 A 1 / 1966 Rinfret (72 ) Inventors : David N . Cook , Brooklyn , NY (US ) ; 3 ,608 ,030 A 11/ 1971 Grant David Arthur Berry , Brookline, MA 4 ,077 , 227 A 3 / 1978 Larson 4 ,205 , 132 A 5 / 1980 Sandine (US ) ; Geoffrey von Maltzahn , Boston , 4 ,655 , 047 A 4 / 1987 Temple MA (US ) ; Matthew R . Henn , 4 ,689 ,226 A 8 / 1987 Nurmi Somerville , MA (US ) ; Han Zhang , 4 ,839 , 281 A 6 / 1989 Gorbach et al. Oakton , VA (US ); Brian Goodman , 5 , 196 , 205 A 3 / 1993 Borody 5 , 425 , 951 A 6 / 1995 Goodrich Boston , MA (US ) 5 ,436 , 002 A 7 / 1995 Payne 5 ,443 , 826 A 8 / 1995 Borody ( 73 ) Assignee : Seres Therapeutics , Inc. , Cambridge , 5 ,599 ,795 A 2 / 1997 McCann 5 . 648 , 206 A 7 / 1997 Goodrich MA (US ) 5 , 951 , 977 A 9 / 1999 Nisbet et al. 5 , 965 , 128 A 10 / 1999 Doyle et al. ( * ) Notice : Subject to any disclaimer , the term of this 6 ,589 , 771 B1 7 /2003 Marshall patent is extended or adjusted under 35 6 , 645 , 530 B1 . 11 /2003 Borody U . -
Carnobacterium Inhibens Isolated in Blood Culture of An
Lo and Sheth BMC Infectious Diseases (2021) 21:403 https://doi.org/10.1186/s12879-021-06095-7 CASE REPORT Open Access Carnobacterium inhibens isolated in blood culture of an immunocompromised, metastatic cancer patient: a case report and literature review Carson Ka-Lok Lo1* and Prameet M. Sheth2,3* Abstract Background: Carnobacterium species are lactic acid-producing Gram-positive bacteria that have been approved by the US Food and Drug Administration and Health Canada for use as a food bio-preservative. The use of live bacteria as a food additive and its potential risk of infections in immunocompromised patients are not well understood. Case presentation: An 81-year-old male with a history of metastatic prostate cancer on androgen deprivation therapy and chronic steroids presented to our hospital with a 2-week history of productive cough, dyspnea, altered mentation, and fever. Extensive computed tomography imaging revealed multifocal pneumonia without other foci of infection. He was diagnosed with pneumonia and empirically treated with ceftriaxone and vancomycin. Blood cultures from admission later returned positive for Carnobacterium inhibens. He achieved clinical recovery with step- down to oral amoxicillin/clavulanic acid for a total 7-day course of antibiotics. Conclusions: This is the fourth reported case of bacteremia with Carnobacterium spp. isolated from humans. This case highlights the need to better understand the pathogenicity and disease spectrum of bacteria used in the food industry for bio-preservation, especially in immunocompromised patients. Keywords: Bacteremia, Carnobacterium inhibens, Carnobacterium species, Case report, Immunocompromised, Sepsis Background bacteria as food additives poses a potential risk for Carnobacterium species are lactic acid-producing, immunocompromised patients, including several studies Gram-positive rod-shaped bacteria that are rarely iso- highlighting cases of bacteremia/sepsis associated with lated in humans and often regarded as non-pathogenic lactic acid bacteria used in probiotics (e.g., Lactobacillus [1]. -
Dynamic Modeling of Carnobacterium Maltaromaticum CNCM I-3298 Growth and Metabolite Production and Model-Based Process Optimization
foods Article Dynamic Modeling of Carnobacterium maltaromaticum CNCM I-3298 Growth and Metabolite Production and Model-Based Process Optimization Cristian Puentes 1,2, Amélie Girardeau 1, Stephanie Passot 1, Fernanda Fonseca 1 and Ioan-Cristian Trelea 1,* 1 INRAE, AgroParisTech, UMR SayFood, Université Paris-Saclay, F-78850 Thiverval-Grignon, France; [email protected] (C.P.); [email protected] (A.G.); [email protected] (S.P.); [email protected] (F.F.) 2 CentraleSupélec, LGPM, Université Paris-Saclay, F-91192 Gif-sur-Yvette, France * Correspondence: [email protected]; Tel.: +33-13-081-5490 Abstract: Carnobacterium maltaromaticum is a species of lactic acid bacteria found in dairy, meat, and fish, with technological properties useful in food biopreservation and flavor development. In more recent years, it has also proven to be a key element of biological time–temperature integrators for tracking temperature variations experienced by perishable foods along the cold-chain. A dynamic model for the growth of C. maltaromaticum CNCM I-3298 and production of four metabolites (formic acid, acetic acid, lactic acid, and ethanol) from trehalose in batch culture was developed using the reaction scheme formalism. The dependence of the specific growth and production rates as well as the product inhibition parameters on the operating conditions were described by the response surface method. The parameters of the model were calibrated from eight experiments, covering a Citation: Puentes, C.; Girardeau, A.; broad spectrum of culture conditions (temperatures between 20 and 37 ◦C; pH between 6.0 and 9.5). Passot, S.; Fonseca, F.; Trelea, I.-C. The model was validated against another set of eight independent experiments performed under Dynamic Modeling of Carnobacterium different conditions selected in the same range. -
Phenotypic and Genotypic Characterization of Carnobacterium
Techno e lo Mokrani et al., Gene Technol 2018, 7:1 n g e y G Gene Technology DOI: 10.4172/2329-6682.1000144 ISSN: 2329-6682 Research Article Open Access Phenotypic and Genotypic Characterization of Carnobacterium divergens Isolated from Refrigerated Tunisian Minced Raw Beef Meat Thouraya Mokrani1, Ines Essid1, Mnasser Hassouna1, Lachheb Jihene2, Ghram Abdeljalil2, and Ahlem Jouini2* 1Unité de Recherche, Food Science and Technology, Higher School of Food Industries of Tunis (ESIAT), University of Carthage, Tunisia 2Laboratory of Epidemiology and Veterinary Microbiology, Pasteur Institute of Tunis (IPT), Université de Tunis El Manar, Tunis, Tunisia Abstract Six psychotropic strains of Carnobacterium divergens were isolated from Tunisian minced raw beef meat packed and stored at 6°C. They were first identified by biochemical methods. Using biochemical reactions and carbohydrate fermentation before their characterisation by molecular techniques. The strain of Carnobacterium divergens is a non- motile, Gram-positive psychotropic rod that lacks catalase, oxidase and mannitol. It grows at pH 9.1 (D-MRS agar), but not on acetate agar (pH ≤ 5.4). For all these isolates, the phenotypic identification, using the API 50 CHL system, revealed a variability in the fermentation abilities of some sugars (glycerol, amygdaline, arbutine, D-trehalose and potassium gluconate). Species-specific polymerase chain reaction (PCR) primers were used to ensure identification of these isolated strains at the species level. Moreover, the sequencing of 16S rRNA gene confirmed that all the six isolates are identified as C. divergens. The Rep-PCR technique was performed to investigate intra-specific diversity within these six strains of C. divergens, commonly identified in meat.