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Blastocyst Stage Extended Culture (Day 5 or Day 6 Transfer)

OVARIAN STIMULATION

A) Stimulation B) Control C) Egg Release The ovaries are stimulated with The response of the ovaries is To assist with the final maturation medication to promote the growth monitored with ultrasounds of the egg and loosening of the egg of follicles containing the eggs. and/or blood tests, to control the from the follicle wall, an injection of size and quantity of follicles. Human Chorionic Gonadotrophin (hCG) - the trigger - is administered. EGG RETRIEVAL D) Egg Retrieval Blastocyst The egg retrieval is performed 35-38 hours after “the trigger” under ultrasound guidance, and The advantage of attempting to grow to the blastocyst stage takes place while you are is that they should have a greater chance of implantation because the sedated. stage of embryo development matches the uterine environment. For to occur, the developing embryo must implant into the lining of the female’s .

E) Sperm Selection Day 0 Egg Pronucleus Day 1 The more morphologically Fertilisation Sperm Pronucleus normal and motile sperm are selected for insemination.

F) Insemination Frozen sperm is thawed, Day 2 Day 3 then added to the eggs or injected into the egg using 2 stage 4 cell stage 8 cell stage Intracytoplasmic Sperm Injection (ICSI) approximately 4 hours after retrieval.

G) Fertilisation Day 3/4 Day 4/5 Day 5/6/7 The dishes are placed Early Blastocyst Expanded Blastocyst in an incubator and checked for fertilisation 16-18 hours after insemination.

Culture

H) Culture Blastocyst Grow in lab It is at the blastocyst stage of development (5 days after fertilisation) that an for 5 days. embryo would normally move out of the and into the uterus.

EMBRYO TRANSFER & I) Embryo Transfer J) Vitrification The embryo chosen for The good-quality transfer is loaded into a embryos that are not transferred are The Luteal Phase is the two-week period between transfer catheter which frozen and stored. Frozen embryos embryo transfer and the pregnancy test. is passed through the can be used in subsequent cycles if into the uterus, and the first cycle is not successful.For You will be encouraged to limit your activity for gently released. Generally, surrogacy, the embryos must be 24 hours after the embryo transfer. only one embryo is quarantined for six months. Frozen transferred, in exceptional embryos can be used once the Your pregnancy blood test will be approximately cases two. quarantine period is finished. 14 days after embryo transfer. ML56 1215

Women contemplating fertility treatment should be aware of the possible risks, some of which are comparable to those of elective surgery. We encourage you to discuss these with your treating specialist.

1300 222 623 rainbowfertility.com.au

Please note: all treatment procedures are carried out in our RTAC (Reproductive Technology Accreditation Committee) accredited fertility clinics, where gametes (eggs/sperm) and embryos are also stored.