Posted on Authorea 4 Jun 2020 | The copyright holder is the author/funder. All rights reserved. No reuse without permission. | https://doi.org/10.22541/au.159129001.11725285 | This a preprint and has not been peer reviewed. Data may be preliminary. r motn etr ftevrs(hped wnpe,Cre,&Mte,18) roiue usually Arboviruses 1989). Mathee, & Cornel, Swanepoel, (Shepherd, virus and the virus of CCHF vectors of important of distribution members are of few in a presence coincidence in the occur The to Romania, shown south-eastern been virus In has the ticks of transmit larval transmission to of transovarial ability generation and succeeding the genera, nature the several the Nevertheless, in to of persistence 2007). ticks females and ixodid al., adult transmission for from et viral demonstrated Wolfel of been means 2003; has principal Gunther, the infection & be Schmitz, to believed Kummerer, are (Drosten, ticks in CCHFV including of ticks, ence ( of species species tick been is various 30 have (22.0%) between than reservoir Africa more and in vector in rates sp natural detected fatality The been CCHF Crimean has 2020). names of (Nasirian, CCHFV the mean hence (33.8%) The Congo Europe as Belgian ob- 1969). and be identified the first to (33.5%) (Casals, in found was combination Asia child was febrile than fever in 1967, lower a in myalgia, used hemorrhagic isolated from are Crimean was weakness, 1956 which Congo in named agent fever, and isolated causative disease, by the virus The and Congo characterized 1944, to in disease 2013). identical Peninsula the fever al., Crimean the et hemorrhagic of in (Bente served human orthonairovirus signs severe hemorrhagic a a and (CCHFV), of virus agent causative fever hemorrhagic Crimean-Congo Romania. in more and level and low identified animals sp. very was of INTRODUCTION at Dermacentor samples surveillance circulates in serological the CCHFV RNA the if of CCHFV on methods None based of biology studies prevalence CCHFV molecular Further used. low known by were 2019. six the testing in genotypes ticks the or county, comprehensive Tulcea of all nil each from of the ticks for highlighting detection sets sp. p=0.05), the primer Rhipicephalus specific <2,5%, for on (prevalence purpose, based pair RT-PCR this positive Time primer For ruminants as Real CCHFV. degenerate domestic using the from tested one detect were and and samples environment to tick genotypes the purpose All Dermacentor from the county. and collected Tulcea with were from sp. Green ticks locations Rhipicephalus SYBR different 127 in five 2019, RNA in July goats) CCHFV In and of based (sheep Romania. were presence south-eastern but the Romania, in investigated in ticks, work (CCHFV) present sp. virus fever The hemorrhagic techniques. Congo Crimean serological of on circulation the suggested studies previous Few Abstract 2020 4, June 2 1 Perot Br˘atuleanuBianca ROMANIA SOUTH-EASTERN FROM SPECIES TICKS DERMACENTOR AND RHIPICEPHALUS IN VIRUS FEVER HEMORRHAGIC CRIMEAN-CONGO OF SURVEY nvriae eSineArcl iMdcn eeiaaInInsud aBa i Iasi din Brad la de Ionescu Ion Veterinara Medicina si Agricole Stiinte de Universitatea Pasteur Institut ik Mhaaa ta. 03.Suiscnutdsnete aecnitnl on relationship a found consistently have then since conducted Studies 2013). al., et (Mehravaran ticks . ylma Dermacentor, Hyalomma, 1 din Anita Adriana , ylmasp. Hyalomma 1 epieChretien Delphine , 2 rgsAnita Dragos , ik,adtedsrbto fhmncsscoeymrosvco distribution vector mirrors closely cases human of distribution the and ticks, ylma hpcpau,Bohls Dermacentor Boophilus, Rhipicephalus, Hyalomma, and Rhipicephalus 2 Hyalomma hogeSavuta Gheorghe , 1 B´eatrice Regnault , eea(elr ont ip aia,1997). Camicas, & Diop, Cornet, (Zeller, genera 1 Hyalomma a ofimdi 04(uirsue l,2014). al., et (Dumitrescu 2014 in confirmed was ik mle htmmeso hsgenus this of members that implies ticks 2 n acEloit Marc and , 1 aa Temmam Sarah , emcno sp Dermacentor Nairoviridae and Ixodes 1 and . 1 Philippe , n h pres- the and ) Riphicephalus aiyi the is family Posted on Authorea 4 Jun 2020 | The copyright holder is the author/funder. All rights reserved. No reuse without permission. | https://doi.org/10.22541/au.159129001.11725285 | This a preprint and has not been peer reviewed. Data may be preliminary. h nert fRA ik eeitoue nRAltrslto Ivtoe,Fac) codn obest to according France), (Invitrogen, solution maintaining For later RT-qPCR. RNA Ticks time in -80degC. real at introduced by stored were analysed was ticks remainder and RNA, II the France) class of and (Paris, a integrity processing Pasteur in Developmental the additional dish, Institut Petri for to ice. surface the used ice-cold transferred in was After an were vials Half on keys. animal scalpels the identification hood. each sterile secondary placing flow morphological using from closed lengthwise, laminar by standard a collected cut using cooled (within was Ticks tick stereomicroscope vials were each in a ticks base. identification, transported under handling, tail were identified Prior ticks and were Live and of udder, stages laboratory. ears labeled. body or performed the the and whole on scrotum was to vials mainly The county container) perineum, separate palpation, Tulcea ruminants. in neck, by alive domestic in infestations the kept tick on sampling of everywere of collected Tick Between presence nape 1 were the segment. ticks the m. for each Engorged along 20 inspected of was was end 1). by sites animal (Table the separated sampled collection at site each was dragging, each each transect of at at every m once covered 10 only dragging, every surface of after The mammals, m examined dragging. was larger 10 flag by and and the small performed N), and and was hectare and birds collection (W Ground-feeding Danube ticks area. (N). river Questing this the Ukraine in by neighboring abundant bordered is are is and livestock, county broadleaf including (E), submediterranean The Sea by shrubs. county. represented Black with Tulcea is sampling the Vegetation pastures across The province. xerophytic Dobrogea sites and of five Romania. forests part from northern south-eastern the ruminants, in forms 44.64 domestic and locations of (latitude: different body-surface county from Tulcea and 2019 environmental July in during consisted collected were Ticks antibodies IgG METHODS studies R˘aileanu, AND previous 2012; Bouloy, MATERIAL in & where Coudrier, in county, Panculescu-Gatej, CCHFV 2015). Tulcea (Ceianu, Savuta, goats of in and & prevalence precisely Anit˘a, sheep Porea, the more in found Romania, assess were from to anti-CCHFV Rollin, collected was Khristova, ticks study (Deyde, CCHFV and this . virus in animals of the problem identify aim of to predominant diversity The used The genetic be high 2006). ticks. can Nichol, in the and in & detected country been detection Ksiazek, a always been viral in never has with CCHFV development has together of RT-qPCR virus (RT-qPCR) circulation infections, reaction the actual chain CCHFV and an polymerase humans CCHFV prove transcription reverse concerning to quantitative essential data real-time is sufficient by several not detection in Virus still days ticks. is 7–15 there to appears Romania donkeys, up 2006). (horses, In and last (Ergonul, mammals small viremia pigs species and Asymptomatic and livestock vertebrate from sheep, animals. isolated numerous cattle, been domestic has goats, in and CCHFV and reported wild species, S- both been animal vertebrate unique tick–vertebrate–tick in has shows pervasive enzootic infection unnoticed which be et in CCHFV strain (Chinikar to nature VII) new Asymptomatic in clade circulates a as virus revealed identified cycles. hemorrhagic (provisionally have Crimean-Congo clade studies unique 2010). Recent a al., constitutes nairoviruses which place. of and takes segments phylogenies family Hoogstraal, RNA segment assembly the 2007; L viral al., and of among most M et 31% members (Estrada-Pena where 2013). 2004). and other phleboviruses) al., 22%, Nichol, As and et 20%, (hantaviruses & (Bente of bunyaviruses 1979). L genome diversity Osborne, other virus Saleh, The sequence than Honig, the of (Aitichou, larger of (L). 2011; degree are M-segments protein two large a and Guzman, nucleocapsid by the L-, and De characterized the S-, of (M) & virus the encodes precursor enveloped Estrada medium segment an the 2005; S (S), is encodes CCHFV the Ibrahim, small segment and & M segments: Gc, Schmaljohn, the approximative McElroy, and three diversity vector of polymerase, acid Gn contains genome arthropod nucleic RNA glycoproteins genome a high an RNA-dependent envelope with a The to the shows virus both and encodes RNA length. rule adapted single-stranded segment this in negative-sense, be follow a kb to not is 19.2 have does virus CCHFV they This but since 2006). species, (Weaver, perhaps host diversity, vertebrate genome a and of levels low show ° S–45.42 ° ;lniue 28.00 longitude: N; Bunyaviridae 2 CF lcpoen agtteGliapparatus, Golgi the target glycoproteins CCHFV , ° W–29.65 emcno sp Dermacentor ° )i iutdi otes Romania southeast in situated is E) and . hpcpau sp Rhipicephalus Posted on Authorea 4 Jun 2020 — The copyright holder is the author/funder. All rights reserved. No reuse without permission. — https://doi.org/10.22541/au.159129001.11725285 — This a preprint and has not been peer reviewed. Data may be preliminary. o h i eoye fCHV h eut r itdi al .A opstv eutwsrecorded, was result positive no As 3. table in listed are negative results as The all prevalence identified were In maximal CCHFV. the qRT-PCR a using of contamination. time defines By real to genotypes this using due reaction. six coding samples results in is tested the RT-PCR due included the that false-positive county, for potential not segment Tulcea any bioterrorism was from have S encountered control sites to from not positive considered collection the have region a being we bp for pathogen, reasons control, used. 4 180 regulatory sets were negative group For a ticks risk primer infectivity. engorged of time a and aerosol specific amplification real as questing its classified the by in with is 2018) genotypes in SybrGreen CCHFV al., CCHFV consisted NP. et on six for CCHFV as (Sas the genotypes based of identified CCHFV of RT-qPCR detection known were detection The six time 47 for the real tested all these, of were purpose, Of detection ticks as this All confirmed collected. For sheeps. were were qRT-PCR. and 80 ticks goats and adult 127 environment from the of from total ticks a questing study a and this 100% During of sensitivity a DISCUSSION assuming estimated AND was RESULTS ticks in 95%. infection of viral level of confidence prevalence maximal negative The of box. A Analysis surfaces. control. analysis and positive Statistical reagents Fifteen a of software. use 96 contamination LightCycler cross to the 3 out with possible with conducted rule was not to data was fluorescence used the it was water bioterrorism, nuclease-free regarding control reasons regulatory For temperature. conditions primers cycling the The on depending Germany). 72 Diagnostics, (annealing) s (Roche 10 95 System 96 follows: as LightCycler used Roche with random performed was 6-mer cDNA 8.6 a and to reagent and transcribed by SybrGReen kit was targeted reagent RNA region These Invitrogen) The Viral One 18090050, Europe. 2018). S-sequence. and – Invitrogen). the al., South (ref. VI N8080127, for – Transcriptase et Europe, (ref. 1248 III Reverse East (Sas primer Africa, to and IV used Central 1068 South – SuperScript degenerate were nucleotide V- II using one from East, others Africa, and Middle was West by and genotypes primers – described Asia CCHFV I these – known genotypes, areas: IV six geographic all Africa, the different West of to of detection assigned each were for the genotypes sets for primer pair specific primer study present the In water Green nuclease-free SYBR mini in on RNeasy recovered based a RNA in with CCHFV The of DNAse purified Detection was on-column Bioanalyzer. RNA extrac- including Agilent shaking -80degC. 4degC. RNA instructions, an at each at total manufacturer’s using stored Between min for the was analyzed used 2 to further France). were for according homogenates and g (Bertin, Germany) The treatment 12,000 Minilys Hilden, at minute. the centrifuged 1 (Qiagen, using were for kit 30s lysates ice on The for cells. placed for times tion. in were Reagent three homogenized samples TRIzol were geographical to the of pools the two procedure ml tick on 1 shaken The containing based were 2). Precellys (Table pooled Ticks from pools were MK28-R 13 ticks tubes of The number reinforced final mL laboratory. a 2 BSL-3 resulting a extraction, in before region conducted were steps Extraction extraction RNA transport. and storing of practices security μ fDA,0.2 DNAc, of l μ flaigbffradte lcrpoee n2 grs e nTi-ctt-DAbuffer. Tris-acetate-EDTA in gel agarose 2% on electrophoresed then and buffer loading of l ° ° o 0 ricbto,floe y4 ylsa 95 at cycles 45 by followed preincubation, s 300 for c o 0sad37 and s 30 for C h pe ag fpeaec a acltduigV ubekSurveillanceTool- Outbreak VS using calculated was prevalence of range upper The : μ fec CFdgpie,0,2 primer, CCHF-deg each of l emcno sp Dermacentor μ 2 eeue,i oa ecinvlm f20 of volume reaction total a in used, were H2O l < ° .%(=.5.W osdrti euta outbcueo h good the of because robust as result this consider We (p=0.05). 2.5% o 0s(oln) h neln eprtr en dutda 60 at adjusted being temperature annealing the (cooling), s 30 for C and . hpcpau sp Rhipicephalus 3 μ fec eoyeseicCH-rmr 10 CCHF-primer, genotype-specific each of l ihcpau sp. Riphicephalus ik sn elTm RT-PCR Time Real using ticks . ° o 0 dntrto) 60 (denaturation), 10s for C μ fPRpoutwsmixed was product PCR of l μ .Tera ieRT-qPCR time real The l. nogdtcs detached ticks, engorged emcno sp Dermacentor ° for C ° μ C l . Posted on Authorea 4 Jun 2020 — The copyright holder is the author/funder. All rights reserved. No reuse without permission. — https://doi.org/10.22541/au.159129001.11725285 — This a preprint and has not been peer reviewed. Data may be preliminary. hr r ayfcosta a nunetecruaino HF.Freape boi aito by variation abiotic example, For demonstrated studies CHHFV. Longitudinal of 2016). al., circulation et (Spengler the evolution CCHFV influence in can reported is providing that region and in (Spengler, factors season infection important many human been are for and has risk There increased host, animals and vertebrate domestic 2016). CCHFV tick, in Rollin, sources localizing to and and & antibodies related humans virus Bergeron, CCHFV between circulating factors interactions of of of the evidence detection range circulation, initial Thus, viral a of when infection. on level cycle occur the tick-vertebrate-tick of ticks silent cases increasing a uninfected align, Clinical suggest behavior may and human data Romania. animals published in previous seronegative host CCHFV with to together on of study transmission found this indicate 2015). of been results directly al., The have (R˘aileanu 1997). not et achieved al., ticks does et not ticks infected (Zeller was attached animals vectors versa: in seropositive to RNA vice virus viral the and the the before study species, that of occurred the overall suggest transmission process In an studies seroconversion the Many the reported and 2015). suggest attached 2015, al., which were (R˘aileanu in et results, ticks ruminants Raileanu negative domestic by obtaining 90 conducted method from CCHFV rRT-PCR testing 74 study samples against by Raileanu recent serum antibodies by antibodies more IgG sheep conducted IgG for a 471 our regarding 27.8% 74% However, tested with studies of of Ceianu consistently prevalence serological 2012). prevalence 2012, a and, few al., obtained In are confirmed et and There not county territory. (Ceianu Tulcea was date. Romanian in Romania to on localities reported from CCHFV different been ticks of not in circulation has virus-infected CCHFV the disease and the human tick-infested of CCHFV of presence results, importation the thus adult and and far, for movement viremia, So hosts short-lived via their 2010). they areas during al., are ticks new et only other into (Gale infect Not livestock virus and Zupanac, virus the 2013). Dedushaj, the Chinikar, introduce (Humolli, amplify & may may regions, occasions also endemic Khakifirouz, several they certain for Haghdoost, on but in important Mostafavi, cases vectors, reservoirs very human 2010; CCHFV are to as Mucaj, animals linked recognized & domestic been epidemiologically However, have been Sheep disease. have virus. clinical and both the cause for of not epidemiology Europe, does the southeastern CCHFV present the in our pathways livestock of In migration area bird geographic active migrations. The fall most 2014). the and the with al., spring of suggest border et international one the (Leblebicioglu the could through Turkey through comprises on (CCHFV) which or in investigations virus is birds fever demonstrated 2013) hemorrhagic migratory that was Crimean-Congo on country spreading ticks Kamarinchev, carried in attached Bulgaria, ticks role & birds’ by In Migratory (Gergova possibly Romania, trade. 1997). evolution livestock in al., virus endemic et the an of Zeller circulation Papadopoulou, Panayotova, has 2013; the Pappa, al., Papa, with CCHFV countries 2004; et al., are Romania, Papa et Bulgaria Honig and 2016; Turkey 2007; Christova, been where al., et & disease, in have (Estrada-Pena the CCHF cases CCHFV 1979). for of the region (Hoogstraal, by of epidemiology endemic majority Hoogstraal caused the an by is about outbreaks 1979 Peninsula review Balkan reported and in detailed The published being Asia, A was and disease 2010). Africa al., and Europe, the et Europe East, distribution, (Chinikar Asia, countries Middle geographic several Africa, widespread in of recorded previously a regions detected has many been fever in has hemorrhagic animals Congo in in Crimean antibodies 2015). RNA al., CCHFV R˘aileanu CCHFV et of 2012; of al., presence prevalence et the low (Ceianu where most county) at (Tulcea or wereregion nil III the sp and highlight Rhipicephalus I study genotypes present for detection The order of the limits from the viruses ten while using (Greece), copies/ VI Two copies/ and 200 study. (Kosovo) this V in (Afghanistan), used IV RT-qPCR the of sensitivity μ epciey(a ta. 08.Drn h aesuyteseict a aoal evaluated favorably was specificity the study same the During 2018). al., et (Sas respectively l usigtcsadegre ik rmsuhesenRmna n21 rmtesame the from 2019 in Romania, south-eastern from ticks engorged and ticks questing . hpcpau sp Rhipicephalus Bunyavirales ik,cletdfo h oiieaias eetse using tested were animals, positive the from collected ticks, . snn ftevrsswsdetected. was viruses the of none as 4 μ eedtce o eoye I(RCongo), (DR II genotypes for detected were l emcno sp. Dermacentor and Posted on Authorea 4 Jun 2020 — The copyright holder is the author/funder. All rights reserved. No reuse without permission. — https://doi.org/10.22541/au.159129001.11725285 — This a preprint and has not been peer reviewed. Data may be preliminary. hnkr . hai .M,Mrd,M,Gy,M . hrai .R,Ziai . oly M. Bouloy, . . . M., Zeinali, Iran. R., in M. fever Shirzadi, hemorrhagic M., Crimean-Congo M. of 10 surveillance Goya, Dis, for and M., Zoonotic evidence distribution Borne Moradi, serologic Geographical M., First S. (2010). Ghiasi, (2012). S., M. Chinikar, Bouloy, Romania. in & virus D., fever doi:10.1089/vbz.2011.0768 Congo Coudrier, hemorrhagic 718-721. and Crimean-Congo I., of fever R. circulation hemorrhagic Panculescu-Gatej, the (2013). Crimean S., M. causing C. Bray, virus Ceianu, & the between A., similarity C. Antigenic Whitehouse, virus. J., (1969). J. A. McAuley, Casals, diver- genetic M., of and D. syndrome Identification clinical Watts, pathogenesis, sity. epidemiology, L., (2005). history, N. S. fever: hemorrhagic Forrester, M. Crimean-Congo A., Ibrahim, D. & Bente, C., RT-PCR. Schmaljohn, real-time one-step K., by doi:10.1016/j.jviromet.2004.10.004 A. viruses 21-26. Puumala McElroy, and S., Seoul, Hantaan, S. Dobrava, Saleh, M., Aitichou, author. corresponding the from request on REFERENCES available interests. are competing study no the declare of We findings the support that data The STATEMENT AVAILABILITY DATA research interests. original competing no no with declare We article review a INTERESTS is page, this OF guidelines author as CONFLICT journal’s required the was on approval noted ethical as No journal, data. the to. and of adhered policies Sciences been ethical have the Agricultural that of confirm authors University The Brad” research. la this de in STATEMENT contribution ETHICS Ionescu R˘at their Alexandra ”Ion for Ioana Dasc˘alu, Romania DVM Department, Mihaela Iasi, Anca Health Medicine DVM Veterinary Public Cozma, Paula the Andreea DVM from thank to wish We longitudinal a Romania. accomplish throughout geographi- to ACKNOWLEDGMENTS epidemiology the order CCHFV expanding in animals in understanding in also better investigations and serological for ticks with years. research collected in correlated previous of research, detected number in of the undertaken not area increasing cal researches was on during focus RNA area, should studies CCHFV this Further from the animals of in identified presence were the antibodies 2019, in that sp highlight results Our species. sampling. animal of competent particular seasons CONCLUSIONS a with and on areas associated load species, often we tick tick is because different and seroprevalence results, exploring vectors, in negative tick Variation our competent sp. the 2019. explain of Hyalomma in partially preference July seroprevalence host also in in distribution, could once, decrease vector This only notable reported ticks a 2010). studies with the al., These antibodies collected et detecting location. (Gale same for period the period winter–spring in optimum performed the was as sampling September repeated when variation considerable and . niia e,100 Res, Antiviral rcScEpBo e,131 Med, Biol Exp Soc Proc emcno sp. Dermacentor ik eenttse,wihi ii forsuy hssuycnteeoeb xeddby extended be therefore can study This study. our of limit a is which tested, not were ticks 1,1919 doi:10.1016/j.antiviral.2013.07.006 159-189. (1), 7,7578 doi:10.1089/vbz.2009.0247 705-708. (7), ik olce rmfiedffrn ie nTle ony vni h CF IgG CCHFV the if even county, Tulcea in sites different five from collected ticks 1,2326 doi:10.3181/00379727-131-33847 233-236. (1), 5 etrBreZooi i,12 Dis, Zoonotic Borne Vector io ehd,124 Methods, Virol J Rhipicephalus Vector (1-2), (9), , oi Posted on Authorea 4 Jun 2020 — The copyright holder is the author/funder. All rights reserved. No reuse without permission. — https://doi.org/10.22541/au.159129001.11725285 — This a preprint and has not been peer reviewed. 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(4), etrBorne Vector 5,769-773. (5), 8,1331- (8), , Posted on Authorea 4 Jun 2020 — The copyright holder is the author/funder. All rights reserved. No reuse without permission. — https://doi.org/10.22541/au.159129001.11725285 — This a preprint and has not been peer reviewed. Data may be preliminary. al .Poigpooo gopn ysml lc,seisadsite) and species place, sample by (grouping protocol Pooling 2. Table county Tulcea in sites ticks collection in tick (1989-1992). fever of Senegal hemorrhagic distribution Bandia, The Crimean-Congo in 1. (1997). Table epizootic L. an J. C. of Camicas, observations Drosten, & 34 field . A., patients. Entomol, ruminants: . Diop, virus and P., . fever Ixodidae) J. R., hemorrhagic (Acari: Cornet, Hewson, Crimean-Congo G., A., in H. P. Zeller, load Leman, viral A., 13 of A. Dis, monitoring Infect Grobbelaar, and Emerg N., detection Petersen, Virus T., (2007). J. Paweska, disease. 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D., Porea, hemorrhagic Anit˘a,R˘aileanu, E., A., C., Doumbali, Crimean-Congo N., to Greece. Zotos, positivity in M., seroprevalence Ntouska, IgG S., with Kallia, associated P., Sidira, A., Papa, 0 ikpo lv u˘,Tle 16 15 origin Sampling 11 5 Rus˘a, Tulcea Slava Rus˘a, Tulcea Slava genus pool Ticks Tick Tulcea Cataloi, 10. 10 pool number Tick Tulcea Ticks Cataloi, 9. 5 pool Tick Pesc˘aresc, Tulcea Sat 8. 5 pool Tick Pesc˘aresc, Tulcea Sat 7. 10 pool Tick Pesc˘aresc, Tulcea Sat 6. 10 pool Tick Pesc˘aresc, Tulcea Sat 5. 7 pool Tick Pesc˘aresc, Tulcea Sat 4. pool Tick Pesc˘aresc, Tulcea Sat 3. pool Tick 2. pool Tick Site 1. type Sample 3,3631 doi:10.4269/ajtmh.1989.40.326 326-331. (3), 5,5156 doi:10.1093/jmedent/34.5.511 511-516. (5), io ehd,255 Methods, Virol J 7,19-10 doi:10.3201/eid1307.070068 1097-1100. (7), ik ikBreDs 4 Dis, Borne Tick Ticks ooa18 47 TOTAL Pesc˘aresc Sat species 18 Tick 27 Somova 17 ticks of Cerchez˘a Number Slava Rus˘a Slava Cataloi Locality 10 84.doi:10.1016/j.jviromet.2018.01.013 38-43. , 127 5,4740 doi:10.1016/j.ttbdis.2013.04.003 417-420. (5), 7 emcno sp. Dermacentor sp. Rhipicephalus sp. Rhipicephalus sp. Rhipicephalus sp. Rhipicephalus LSNg rpDs 10 Dis, Trop Negl PLoS hpcpau sp. Rhipicephalus sp. Rhipicephalus sp. Rhipicephalus sp. Rhipicephalus sp. Dermacentor sp. Dermacentor sp. Dermacentor sp. Dermacentor sp. Dermacentor sp. Dermacentor urTpMcoilImnl 299 Immunol, Microbiol Top Curr dl sheeps Adult goats Adult goats Adult sheeps Adult Environment Environment Environment Environment Environment Environment mJTo Med Trop J Am 1,e0004210. (1), Epid´emiol. ´ Med J , Posted on Authorea 4 Jun 2020 — The copyright holder is the author/funder. All rights reserved. No reuse without permission. — https://doi.org/10.22541/au.159129001.11725285 — This a preprint and has not been peer reviewed. Data may be preliminary. CF ntcsfo ucacounty Tulcea of from presence ticks the in for CCHFV RT-qPCR using obtained Results 3. Table apetp ieTcsnme ik eu apigorigin Sampling genus Ticks number Ticks 15 Cerchez˘a, Tulcea Slava pool Tick Site 11. type Sample 3 ikpo ooa uca13 5 Tulcea Somova, Tulcea Somova, pool Tick 13. pool Tick 12. oaiyTc eu o fCHVpstv ticks positive CCHFV of No. Pesc˘arescSat Somova genus Cerchez˘a Tick Slava Rus˘aSlava Cataloi Locality emcno sp. Dermacentor sp. Riphicephalus sp. Riphicephalus sp. Riphicephalus sp. Riphicephalus 8 0/47 0/18 0/17 0/27 0/18 hpcpau sp. Rhipicephalus sp. Rhipicephalus sp. Rhipicephalus dl goats Adult sheeps Adult goats Adult