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XXX-Reunión-Anual-SBC.Pdf BIOCELL ISSN 0327 - 9545 2012, 36(3): A99-A160 PRINTED IN ARGENTINA CUYO BIOLOGY SOCIETY (Sociedad de Biología de Cuyo) Abstracts from the XXX Annual Meeting In Memorian Dr. Mario H. BURGOS December 6 - 7, 2012 Potrero de los Funes, San Luis, Argentina Abstracts were revised by the Scientific Commitee A-100 ABSTRACTS BIOCELL 36(3), 2012 Board of the Cuyo Biology Society PRESIDENTE Dra. Lucia Fuentes VICEPRESIDENTE Dra. Nora Arenas SECRETARIA Dra. Eugenia Ciminari TESORERA Lic. María Verónica Pérez Chaca VOCALES TITULARES Dr. Edgardo Álvarez Dr. Miguel Fornés Dr. Susana García Aseff VOCALES SUPLENTES Dra. Adriana Telechea Dra. Claudia Castro REVISORES DE CUENTA Titular: Dra. Marta S. Ojeda Suplente: Dr. Walter Manucha Organizing Committee Dra. Lucia Fuentes Lic. M. Verónica Pérez Chaca Dra. Marta S. Ojeda Dra. Eugenia Ciminari Dra. Susana García Aseff Farm. Mauricio Tévez Bioq. Maximiliano Chaves Dra. Nora Arenas Dra. Adriana Telechea Scientific Committee Dra. Graciela Nora Arenas Dra. Marta Moglia Dra. Gladys Ciuffo Dra. Liliana Oliveros Dra. Teresa Cortiñas Dra. Marta Susana Ojeda Dra. María Teresa Damiani Dra. Nora Pappano Dr. Luis Del Vitto Dra. Ana María Rastrilla Dra. Lucia Fuentes Dr. Fernando Saraví Dr. Ricardo Masuelli Dra. Adriana Telechea BIOCELL 36(3), 2012 ABSTRACTSLECTURES A-101 L.1. SHIGA TOXIN ACTION IN TARGET ORGANS. STRATEGIES FOR PREVENTION OF HEMOLYTIC UREMIC SYNDROME Ibarra C. Departamento de Fisiología, Facultad de Medicina, UBA. Investigador Principal CONICET. E-mail: [email protected] Gastrointestinal infection with STEC/EHEC strains causes diarrhea and hemorrhagic colitis, and in addition, Hemolytic Uremic Syndrome (HUS) defined by a triad of hemolytic anemia, thrombocytopenia, and acute renal failure. HUS develops in 10%-15% of small children several days after bloody diarrhea and is the most common cause of acute renal failure in infants in Argentina. It is transmitted to humans primarily through consumption of contaminated foods, such as raw or undercooked ground meat products or direct contact with cattle. Because STEC are non-invasive, it is generally accepted that Shiga toxin (Stx) must be absorbed from the intestine to cause HUS by different mechanisms that contribute to the intestinal pathology. Renal damage has been strongly associ- ated with Stx, which binds to the globotriaosylceramide (Gb3) receptor on the plasma membrane of epithelial and endothelial cells causing cellular death. We have shown that kidney lesions similar to those described in humans may be obtained in rats treated with Stx2 and that the kidney was protected by previous treatment with inhibitors of Gb3 synathesis. Furthermore, we have shown that Stx2 interrupted pregnancy in rats and we speculate that STEC infections during pregnancy could be detrimental to human fetuses. L.2. ATTENUATED MUTANT OF Mycobacterium bovis AS EXPERIMENTAL VACCINE AGAINST BOVINE TUBERCULOSIS Blanco F, Bianco MV, Garbaccio F, Meikle V, Gravisaco MJ, Montenegro V, Canal A, Vagnoni L, Buddle B, Bigi F, Cataldi A. Instituto de Biotecnología, INTA, Argentina. E-mail: [email protected] Introduction: Mycobacterium bovis is the causative agent of animal or bovine tuberculosis. This mycobacteria is highly related to M. tuberculosis, the Koch bacilli. M. bovis can cause tuberculosis in many animals including humans. Currently, there are no vaccines against bovine tuberculosis. Objective: to test M. bovis mutant obtained by homologous recombination as experimental anti TB vaccine in cattle. Results: A M. bovis strain, deleted in the yrbE2B and mceA2 genes (M. bovis Δmce2) was tested as an experimen- tal vaccines in cattle that was subsequently challenged with virulent M. bovis. Groups of 5/6 calves 3,5 month-old were vaccinated and challenged 9 weeks later with a virulent strain of M. bovis by intratracheal route. Also a non- vaccinated group and a group vaccinated with BCG were included as controls. Along the assay samples were taken to measure IFNg by an IGRA test, cytometry and cytokine responses of PPD restimulated PBMC. IGRA test showed IFNg values similar to that in pre-vaccination except for the animals vaccinated with M. bovis Δmce2, where an increase was observed at 30 dpv. The animals were euthanised and necropsied 18 weeks after challenge. The group vaccinated with M. bovis Δmce2 was the one with lowest total score of lesions in lungs and lymphnodes. Histopathology of lungs and lymphnodes showed in the M. bovis Δmce2 and BCG vaccinated groups a marked reduction of the severity of histopathological lesions. A positive reaction to tuberculin intradermal test after vac- cination was observed in animals vaccinated with M. bovis Δmce2 but not with BCG or M. bovis ΔlprG. In contrast, after challenge, non vaccinated animals gave induration diameters higher than non vaccinated controls. Conclusions: In summary, M. bovis Δmce2 is a promising vaccine candidate to control M. bovis pathogenesis in cattle. A-102 ABSTRACTSLECTURES BIOCELL 36(3), 2012 L.3. INFLUENCE OF THE CUMULUS AND GONADOTROPINS ON THE METABOLIC PROFILE OF PORCINE CUMULUS-OOCYTE COMPLEXES DURING IN VITRO MATURATION Alvarez G. Cátedra de Química Biológica. Facultad de Ciencias Veterinarias. Universidad de Buenos Aires. Argentina. The substrate availability during in vitro culture of cumulus oocyte complexes (COCs) strongly impacts on oocyte maturation. Several aspects of porcine COC metabolism during in vitro maturation remain to be elucidated. The aim of the work was to examine the influence of the cumulus and gonadotropins on the metabolic profile of porcine COCs during in vitro maturation. Immature COCs were assigned to morphological classes A1 (with a dense cumulus), A2 (with a translucent cumulus), B1 (with the corona radiata), B2 (with only some remaining cumulus cells) and matured with or without gonadotropins. Glycolysis and ammonia production were higher in the A class COCs. Gonadotropins increased both, especially in the A1 COCs (P < 0.05). The A class COCs had the highest initial protein contents and at the end of in vitro maturation. Furthermore, hormonal stimulation induced a similar increase in protein contents of both A classes (P < 0.05). The neutral lipid content and ROS levels were similar in the immature oocytes of the COCs of all classes. A reduction was seen in both these variables when maturation proceeded either in the presence or absence of gonadotropins. The cumulus type surrounding the oocyte is related to the metabolism of carbohydrates and amino acids by the COC during in vitro maturation under gonadotropic stimulation. Oocyte lipolytic activity and ROS production appear to be independent of the surrounding cumulus and the presence of gonadotropins. L.4. SUNFLOWER IMIDAZOLINONE RESISTANCE: GENE EXPRESSION ANALYSIS AND BIOCHEMI- CAL SCREENING Vega T, Breccia G, Gil M, Ochogavía A, Felitti S, Picardi L, Nestares G. Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Campo Exp. Villarino, Zavalla, Santa Fe, Ar- gentina. Acetohydroxyacid synthase (AHAS) catalyzes the first reaction in branched chain amino acids biosynthesis. This enzyme is of substantial importance because it is the target of several herbicides, including all members of the sulfonylurea and imidazolinone (IMI) families. In sunflower, IMI resistance was introgressed from a wild popula- tion into elite inbred lines. Imr1 and Imr2 was proposed as the genetic base of IMI-resistance inheritance, where Imr1 is an allelic variant of the ahasl gene coding for the catalitic subunit of AHAS. Imr2 is a modifier gene but his effect remains unknown. The objectives of this work were to study the early response to imidazolinones at a whole-plant and biochemical levels, to evaluate the ahas expression pattern, and to determine the resistance mecha- nism related to Imr2. Genotypes differing in IMI resistance showed diferential growth parameters and AHAS in vivo activity levels in response to IMI tratments. This allowed to discriminate among genotypes and have a pos- sible use as a selection method for evaluating AHAS inhibitor resistance. Relative transcripts levels of ahas genes were measured through RT-qPCR in leaf and root tissues of control and imazapyr-treated plantlets. Levels of expression in these tissues were consistent with previously observed in vivo and in vitro AHAS activity. AHAS response to P-450 inhibitors in combination with IMI was also evaluated. The effect of inhibitors in resistant plantlets suggested that a detoxification, P-450-mediated mechanism might be implicated in herbicide resistance. This mechanism could be related to the modifier effect of Imr2 gene in this species. BIOCELL 36(3), 2012 ABSTRACTSLECTURES A-103 L.5. ARTIFICIAL WETLANDS AND THEIR IMPORTANCE IN BIRD CONSERVATION Echevarria AL. Fundación Miguel Lillo, Miguel Lillo 251, (4000). Tucumán. E-mail: [email protected] Artificial wetlands are dynamic ecosystems due to changes in water level designed by man. Over time, reservoirs become wintering sites for migratory birds and breeding sites for resident birds, a new role not predictedin their construction. As an example, plovers and sandpipers fly through our country during spring and summer and use beaches, superficial and deeper waters as food supply sites. Studies of bird communities and their variations are useful in the determination of the role wetlands play in the conservation of biodiversity in the biomes they are located in. Bird communities of many artificial reservoirs from northwestern Argentina were studied during 2 decades. Censuses were
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