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Mycobacterial Species As Case- Study of Comparative Genome Analysis

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Mycobacterial Species As Case- Study of Comparative Genome Analysis 2012 [SCIENTIFIC PRODUCTION] Doctorat Es Science in Microbiology & Molecular Biology Scientific production 2012 SCIENTIFIC PRODUCTION A- List of international Publications (06): 1. Fathiah ZAKHAM, Lamiae BELAYACHI, Dave USSERY, Mohammed AKRIM, Abdelaziz BENJOUAD, Rajae El AOUAD and Moulay Mustapha ENNAJI. 2011. Mycobacterial species as a case-study of comparative genome analysis. Cell. Mol. Biol. 57 : 1462-1469 . 2. Fathiah ZAKHAM , Halima BAZOUI, Mohammed AKRIM, Sanae LAMRABET, Ouafae LAHLOU, Mohamed EL MZIBRI, Abdelaziz BENJOUAD, My Mustapha ENNAJI and Rajae ELAOUAD. 2012. Evaluation of conventional Molecular diagnosis of Mycobacterium tuberculosis in the clinical specimens from Morocco. J Infect Dev Ctries. 6(1):40-45. 3. Fathiah ZAKHAM , Mohammed AKRIM, Mohamed ELMZIBRI, Abdelaziz BENJOUAD, Rajae ELAOUAD and My Mustapha ENNAJI. 2012 . Rapid Screening and Diagnosis of Tuberculosis: a real Challenge for the mycobacteriologist. Cell. Mol. Biol . 58 : 1632-1640. 4. Fathiah ZAKHAM, Oufae LAHLOU, Mohammed AKRIM, Nada BOUKLATA, Sanae JAOUHARI, Mohamed ELMZIBRI, Abdelaziz BENJOUAD, Mustapha ENNAJI and Rajae ELAOUAD. 2012. Comparison of a DNA based PCR approach with conventional methods for the detection of Mycobacterium tuberculosis in Morocco . Mediterr J Hematol Infect Dis. 4: (1) 5. Fathiah ZAKHAM , Othmane AOUANE, David USSERY, Abdelaziz BENJOUAD and Mouly Mustapha ENNAJI. 2012. Computational and comparative genomics- proteomics and Phylogeny analysis of twenty one mycobacterial genomes. BMC microbial informatics and experimentation. 7:2. 6. Fathiah ZAKHAM , Imane CHAOUI, Mohammed ABID, Moulay Driss MESSAOUDI, Moulay Mustapha ENNAJI, Mohamed ELMZIBRI. Automated sequencing for the direct detection of Multi drug Mycobacterium tuberculosis strains in the sputum specimens of Moroccan patients. (In preparation). Doctorat Es Science in Microbiology & Molecular Biology Scientific production 2012 B- Publication of sequences in GenBank at National Center for Biotechnology Information NCBI (10) HSP65 VFS22 04 11.sqn HSP65S4 JF921153 HSP65 VFS22 04 11.sqn HSP65S2 JF921154 HSP65 VFS22 04 11.sqn HSP65S3 JF921155 HSP65 VFS22 04 11.sqn HSP65S5 JF921156 HSP65 VFS22 04 11.sqn HSP65S7 JF921157 HSP65 VFS22 04 11.sqn HSP65S8 JF921158 HSP65 VFS22 04 11.sqn HSP65S9 JF921159 HSP65 VFS22 04 11.sqn HSP65S6 JF921160 HSP65 VFS22 04 11.sqn HSP65S10 JF921161 HSP65 VFS22 04 11.sqn HSP65S1 JF921162 C- List of Communications (13): 1. Oral presentations (05) 1. Fathiah ZAKHAM . Diagnostic moléculaire de Mycobacterium tuberculosis dans les échantillons cliniques. The 8 th edition of Doctorial in Morocco. Incubateur Universitaire de Marrakech « INMA ». 12-18 th December 2010. 2. Fathiah ZAKHAM , Lamiae BELAYACHI, Dave USSERY, Mohammed AKRIM, Abdelaziz BENJOUAD, Rajae ElAOUAD and M M ENNAJI . International Bioinformatics Software School. Tangier- Morocco. Comparative Genomics and Proteomics for differentiation between fourteen Mycobacterial strains. IBSS 2011, 4-9th April 2011 3. Fathiah ZAKHAM , Halima BAZOUI, Mohammed AKRIM, Sanae LAMRABET, Ouafae LAHLOU, Mohamed ELMZIBRI, Abdelaziz BENJOUAD, Rajae ELAOUAD and My Mustapha ENNAJI. Evaluation of conventional polymerase chain reaction for the diagnosis of Mycobacterium tuberculosis in the Doctorat Es Science in Microbiology & Molecular Biology Scientific production 2012 clinical specimens from Morocco. International Congress: La recherché, la Biotechnologie et le consommateur au service de l’Environnement et de l’industrie Agroalimentaire » Faculty of Science Kenitra. 19 th -20 th May 2011. 4. Fathiah ZAKHAM , Othmane AOUANE, David USSERY, Abdelaziz BENJOUAD and Mouly Mustapha ENNAJI. Computational genome and proteome analysis of Mycobacterium tuberculosis and non tuberculosis mycobacteria. International conference Humboldt Kolleg “New Prospects and Challenges for Science and Education in the MENA region. Marrakesh- Morocco. 9th - 11 th March, 2012. 5. Fathiah ZAKHAM , Imane CHAOUI, Mohammed ABID, Moulay Driss MESSAOUDI, Moulay Mustapha ENNAJI, Mohamed ELMZIBRI. Rapid detection of Multi Drug Resistance Mycobacterium tuberculosis strains in the clinical specimens from Morocco. International conference on Antimicrobial Research. Lisbon, Portugal. 21 st -23 rd November, 2012. (accepted) 2. Poster presentations (08) 1. Fathiah ZAKHAM , Halima BAZOUI , M M Ennaji, Sanae LAMRABET, Ouafae LAHLOU, Mohamed ELMZIBRI, Abdelaziz BENJOUAD, Mohammed AKRIM, and Rajae ELAOUAD. 3rd International Congress of Biochemistry and Molecular Biology. Direct Molecular Diagnosis of Mycobacterium tuberculosis in the sputum specimens. Marrakech, April 20-25 th , 2009. 2. Fathiah Zakham , Lamiae BELAYACHI, Dave USSERY, Mohammed AKRIM, Abdelaziz BENJOUAD, Rajae El AOUAD and Moulay Mustapha ENNAJI. Xth Spanish symposium on Bioinformatics. Comparative Genome Analysis of Mycobacterium sp (Mycobacterium Tuberculosis and other non tuberculosis Mycobacteria). Torremolinos- Malaga, Spain 27-29th . October, 2010. 3. Fathiah ZAKHAM , Lamiae BELAYACHI, Dave USSERY, Mohammed AKRIM, Abdelaziz BENJOUAD, Rajae El AOUAD and Moulay Mustapha ENNAJI. International Bioinformatics Software School IBSS 2011. Computational Genomics and Proteomics are potential tools for the comparison Doctorat Es Science in Microbiology & Molecular Biology Scientific production 2012 between pathogenic and free living Mycobacteria. Tangier- Morocco 4-9th April, 2011. 4. Fathiah ZAKHAM , Oufae LAHLOU, Mohammed AKRIM, Nada BOUKLATA, Sanae JAOUHARI, Mohamed ELMZIBRI, Abdelaziz BENJOUAD, Mustapha ENNAJI and Rajae ELAOUAD. The insertion sequence IS6110 as a potential tool for the detection of Mycobacterium tuberculosis in the critical cases of the Moroccan population. The 7 th European congress of Tropical Medicine and International Health. Barcelona. Spain.3- 6th October. 2011. 5. Fathiah ZAKHAM , Oufae LAHLOU, Mohammed AKRIM, Mohamed ELMZIBRI, Abdelaziz BENJOUAD, Rajae ELAOUAD and Mustapha ENNAJI. Detection of Mycobacterium tuberculosis in the clinical specimens by the PCR technique targeting the insertion sequence IS6110 in Morocco. International engagement: Responsible Bio Science and Secure Society, workshop III. Tunis, Tunisia, 31 st October- 1st November, 2011. 6. Fathiah ZAKHAM , Mohammed AKRIM, Nada BOUKLATA, Mohamed ELMZIBRI, Abdelaziz BENJOUAD, Mustapha ENNAJI and Rajae ELAOUAD. Comparative analysis of conventional and molecular detection of Mycobacterium tuberculosis in the critical cases of the Moroccan population. International conference Humboldt Kolleg “New Prospects and Challenges for Science and Education in the MENA region. Marrakesh- Morocco. 9th - 11 th March, 2012. 7. Fathiah ZAKHAM , Othmane AOUANE, David USSERY, Abdelaziz BENJOUAD, Mohamed ELMZIBRI and Mouly Mustapha ENNAJI. Evolutionary Relationships between pathogenic and free living mycobacterial species. Tuberculosis Conference 2012: Biology, Pathogenesis, Intervention strategies. Pasteur Institute Paris. 12 th -15 th September 2012. 8. Fathiah ZAKHAM , Imane CHAOUI, Mohammed ABID, Moulay Driss MESSAOUDI, Moulay Mustapha ENNAJI, Mohamed ELMZIBRI. Automated sequencing for the rapid detection of Multi Drug Resistant Mycobacterium tuberculosis strains in the sputum specimens of Moroccan patients. Tuberculosis Conference 2012: Biology, Pathogenesis, Intervention strategies. Pasteur Institute Paris. 12 th -15 th September 2012. Doctorat Es Science in Microbiology & Molecular Biology Dedication 2012 DEDICATION I dedicdedicateate my modest work to every person who helpedhelped me in the process of fulfilling my thesis. To my daughtedaughterr Romissa’a , my symbol of love To my parents, my symbol of confidence To my sisterssisters:: Najat, Mariam and FatimaFatima,, my symbol of kindness To my friends, my symbol of fidelity and respect And to whom I’m grateful.grateful. Fathiah ZAKHAM Molecular Diagnosis of MTB in clinical specimens F. ZAKHAM Doctorat Es Science in Microbiology & Molecular Biology Foreword & Acknowledgement 2012 FOREWORD & ACKNOWLEDGEMENT This present study was done within the framework between laboratory of Biochemistry and Immunology at Faculty of Science-Rabat (FSR)-University Mohammed V-AGDAL, directed by Pr. Abdelaziz BENJOUAD, the laboratory of Virology and Hygiene &Microbiology (LVHM) at Faculty of Sciences and Techniques (FSTM) - University Hassan II Mohammedia, Casablanca (UH2MC), directed by Pr. Moulay Mustapha Ennaji and Laboratory of Molecular Biology at the National Institute of Hygiene (NIH) of Rabat. This thesis was realized in collaboration with the Unit of Biology & Medical Research at the National Centre of Nuclear Energy, Sciences & Techniques (CNESTEN) of Rabat. The computational & bioinformatics analysis were performed in partnership with the Centre for Biological Sequence Analysis (CBS) - Technical University of Denmark, Lyngby, Denmark. This thesis was financially supported by the Academy Hassan II of Sciences & Techniques within the framework of IMMGEN project and The Ministry of Higher Education and Scientific Research (UH2MC) and the National Centre of Scientific and technical research (CNRST) for supporting a part of technical analysis. I want to express my sincere thanks to all, who had played a role in the drafting of this manuscript and helped bring this work into the light. My regards and my thanks are due to: Professor Abdelaziz BENJOUAD, the Director of the CNRST, the head of the UFR Biochemistry and immunology, who co supervised and directed me through the period of my study. In addition, I would like to thank Pr BENJOUAD for his commitment and his competence,
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