DOCSLIB.ORG

Molecular Phylogeny of the Genus Metrosideros And

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Molecular Phylogeny of the Genus Metrosideros And MOLECULAR PHYLOGENY OF THE GENUS METROSIDEROS AND POPULATION GENETICS OF SOME NEW ZEALAND SPECIES WITHIN THE GENUS A thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy in Biology in the University of Canterbury by Kumilign Asmare Melesse University of Canterbury 2017 Acknowledgment First of all, I would like to thank the almighty God for keeping me healthy and giving me the stamina to pursue and complete my PhD study in the field of genetics, which I have liked most since my school days. I would also like to thank my supervisors Assoc Professor Hazel Chapman from University of Canterbury and Dr Gary Houliston from Landcare Research at Lincoln for their wonderful and uninterrupted supervision and support in every step of my research progress. Without their consistent advice and supervision, the completion of my study would not have been realized. I would like to thank Dr Pieter Pelser from University of Canterbury for extending his unreserved help in gap coding of nucleotide sequence data that were used for the molecular phylogeny work, and also for the valuable discussions I had with him regarding the Bayesian and Maximum Parsimony tree building methods. My thanks also go to Landcare Research at Lincoln for providing me the opportunity to use the molecular lab and the reagents therein for all my PhD research, and also for granting me access to Allan Herbarium to take leaf samples of Metrosideros species for my molecular phylogeny work. I am grateful to Dr Dagmar Goeke, Caroline Mitchell and Katherine Trought for their technical support in the Molecular Lab at Landcare Research, which facilitated my lab work and also made my stay at Landcare Research enjoyable. I would like to thank the University of Canterbury (UC) for granting me the financial support (UC Doctoral Scholarship) to pursue my PhD. Last but not least, I would like to thank Drs Peter de Lange and Jeremy Rolfe from the Department of Conservation for providing leaf samples of Metrosideros bartlettii trees for DNA extraction for genetic diversity analysis. ii Table of Contents Acknowledgment ...................................................................................................................... ii Table of Contents .................................................................................................................... iii List of Figures ......................................................................................................................... vii List of Tables ............................................................................................................................ ix Chapter 1: General Introduction ............................................................................................ 1 1.1 Resolving phylogenetic relationships in the genus Metrosideros....................................... 3 1.2 Population genetics of Metrosideros bartlettii ................................................................... 5 1.3 Genetic confirmation of hybridisation between Metrosideros excelsa and M. robusta ..... 6 1.4 Summary of key findings .................................................................................................... 7 1.5 Future research directions ................................................................................................. 10 1.6 Implications of myrtle rust on Metrosideros bartlettii conservation ................................ 11 References ................................................................................................................................. 14 Chapter 2: Molecular phylogeny of the the genus Metrosideros ......................................... 27 Abstract ..................................................................................................................................... 27 2.1 Introduction ....................................................................................................................... 27 2.2 Molecular markers, phylogenetic tree building methods, and nucleotide substitution models ............................................................................................................................... 32 2.2.1 Molecular markers used in the study ..................................................................... 32 2.2.2 Phylogenetic tree building methods ....................................................................... 40 2.2.2.1 Distance-based methods........................................................................... 40 2.2.2.2 Character-based methods ......................................................................... 42 2.2.3 Nucleotide substitution models .............................................................................. 46 2.3 Materials and Methods ...................................................................................................... 49 2.3.1 Acquisition and alignment of nucleotide sequence data ........................................ 49 2.3.2 Data analysis .......................................................................................................... 50 iii 2.4 Results ............................................................................................................................... 51 2.4.1 Results of nuclear sequence data ........................................................................... 51 2.4.2 Results of chloroplast data ..................................................................................... 56 2.5 Discussion ......................................................................................................................... 58 2.5.1 Phylogenetic analysis of the genus Metrosideros .................................................. 58 2.5.1.1 Phylogeny of the subgenus Metrosideros ................................................ 58 2.5.1.2 Phylogeny of the subgenus Mearnsia ...................................................... 64 2.5.1.3 Phylogeny of the subgenus Carpolepis.................................................... 67 2.5.1.4 Phylogenetic analysis of Chloroplast data ............................................... 68 2.5.2 Dispersal routes ...................................................................................................... 71 2.5.2.1 Dispersal routes for the subgenus Metrosideros ...................................... 71 2.5.2.2 Dispersal routes for the subgenus Mearnsia ............................................ 75 2.5.2.3 Dispersal conclusions............................................................................... 77 References ................................................................................................................................. 81 Chapter 3: Genetic diversity and structure of Metrosideros bartlettii as revealed by AFLP and SSR markers .................................................................................... 103 Abstract ................................................................................................................................... 103 3.1 Introduction .................................................................................................................... 103 3.1.1 Description of Metrosideros bartlettii ................................................................. 103 3.1.2 Background and significance of the study ........................................................... 106 3.1.3 Molecular markers used in the study ................................................................... 108 3.1.3.1 Amplified Fragment Length Polymorphism (AFLP) ............................ 108 3.1.3.2 Simple Sequence Repeats (SSR) ........................................................... 110 3.2 Materials and Methods .................................................................................................... 111 3.2.1 Plant material ....................................................................................................... 111 3.2.2 DNA Extraction and AFLP reaction .................................................................... 114 iv 3.2.3 SSR Reaction ....................................................................................................... 115 3.3 Data analysis ................................................................................................................... 116 3.3.1 Genetic diversity .................................................................................................. 116 3.3.2 Genetic structure .................................................................................................. 118 3.4 Results ............................................................................................................................. 122 3.4.1 Genetic diversity .................................................................................................. 122 3.4.2 Genetic structure .................................................................................................. 124 3.5 Discussion ....................................................................................................................... 130 3.5.1 Genetic diversity .................................................................................................. 130 3.5.2 Genetic structure .................................................................................................. 138 3.5.3 Implications for M. bartlettii conservation .........................................................
Load more

Recommended publications
  • The Plant Press
    Special Symposium Issue continues on page 14 Department of Botany & the U.S. National Herbarium The Plant Press New Series - Vol. 20 - No. 3 July-September 2017 Botany Profile Plant Expeditions: History Has Its Eyes On You By Gary A. Krupnick he 15th Smithsonian Botani- as specimens (living or dried) in centuries field explorers to continue what they are cal Symposium was held at the past. doing. National Museum of Natural The symposium began with Laurence T he morning session began with a History (NMNH) and the U.S. Botanic Dorr (Chair of Botany, NMNH) giv- th Garden (USBG) on May 19, 2017. The ing opening remarks. Since the lectures series of talks focusing on the 18 symposium, titled “Exploring the Natural were taking place in Baird Auditorium, Tcentury explorations of Canada World: Plants, People and Places,” Dorr took the opportunity to talk about and the United States. Jacques Cayouette focused on the history of plant expedi- the theater’s namesake, Spencer Baird. A (Agriculture and Agri-Food Canada) tions. Over 200 participants gathered to naturalist, ornithologist, ichthyologist, and presented the first talk, “Moravian Mis- hear stories dedicated col- sionaries as Pioneers of Botanical Explo- and learn about lector, Baird was ration in Labrador (1765-1954).” He what moti- the first curator explained that missionaries of the Mora- vated botanical to be named vian Church, one of the oldest Protestant explorers of at the Smith- denominations, established missions the Western sonian Institu- along coastal Labrador in Canada in the Hemisphere in the 18th, 19th, and 20th tion and eventually served as Secretary late 1700s.
    [Show full text]
  • Phylogenetic Models Linking Speciation and Extinction to Chromosome and Mating System Evolution
    Phylogenetic Models Linking Speciation and Extinction to Chromosome and Mating System Evolution by William Allen Freyman A dissertation submitted in partial satisfaction of the requirements for the degree of Doctor of Philosophy in Integrative Biology and the Designated Emphasis in Computational and Genomic Biology in the Graduate Division of the University of California, Berkeley Committee in charge: Dr. Bruce G. Baldwin, Chair Dr. John P. Huelsenbeck Dr. Brent D. Mishler Dr. Kipling W. Will Fall 2017 Phylogenetic Models Linking Speciation and Extinction to Chromosome and Mating System Evolution Copyright 2017 by William Allen Freyman Abstract Phylogenetic Models Linking Speciation and Extinction to Chromosome and Mating System Evolution by William Allen Freyman Doctor of Philosophy in Integrative Biology and the Designated Emphasis in Computational and Genomic Biology University of California, Berkeley Dr. Bruce G. Baldwin, Chair Key evolutionary transitions have shaped the tree of life by driving the processes of spe- ciation and extinction. This dissertation aims to advance statistical and computational ap- proaches that model the timing and nature of these transitions over evolutionary trees. These methodological developments in phylogenetic comparative biology enable formal, model- based, statistical examinations of the macroevolutionary consequences of trait evolution. Chapter 1 presents computational tools for data mining the large-scale molecular sequence datasets needed for comparative phylogenetic analyses. I describe a novel metric, the miss- ing sequence decisiveness score (MSDS), which assesses the phylogenetic decisiveness of a matrix given the pattern of missing sequence data. In Chapter 2, I introduce a class of phylogenetic models of chromosome number evolution that accommodate both anagenetic and cladogenetic change.
    [Show full text]
  • "National List of Vascular Plant Species That Occur in Wetlands: 1996 National Summary."
    Intro 1996 National List of Vascular Plant Species That Occur in Wetlands The Fish and Wildlife Service has prepared a National List of Vascular Plant Species That Occur in Wetlands: 1996 National Summary (1996 National List). The 1996 National List is a draft revision of the National List of Plant Species That Occur in Wetlands: 1988 National Summary (Reed 1988) (1988 National List). The 1996 National List is provided to encourage additional public review and comments on the draft regional wetland indicator assignments. The 1996 National List reflects a significant amount of new information that has become available since 1988 on the wetland affinity of vascular plants. This new information has resulted from the extensive use of the 1988 National List in the field by individuals involved in wetland and other resource inventories, wetland identification and delineation, and wetland research. Interim Regional Interagency Review Panel (Regional Panel) changes in indicator status as well as additions and deletions to the 1988 National List were documented in Regional supplements. The National List was originally developed as an appendix to the Classification of Wetlands and Deepwater Habitats of the United States (Cowardin et al.1979) to aid in the consistent application of this classification system for wetlands in the field.. The 1996 National List also was developed to aid in determining the presence of hydrophytic vegetation in the Clean Water Act Section 404 wetland regulatory program and in the implementation of the swampbuster provisions of the Food Security Act. While not required by law or regulation, the Fish and Wildlife Service is making the 1996 National List available for review and comment.
    [Show full text]
  • Metrosideros.Pdf
    Riding the ice age El Nin˜ o? Pacific biogeography and evolution of Metrosideros subg. Metrosideros (Myrtaceae) inferred from nuclear ribosomal DNA S. D. Wright*†, C. G. Yong*, J. W. Dawson‡, D. J. Whittaker*, and R. C. Gardner* *School of Biological Sciences, University of Auckland, P.B. 92019 Auckland, New Zealand; and ‡School of Biological Sciences, Victoria University, Box 600 Wellington, New Zealand Edited by Peter H. Raven, Missouri Botanical Garden, St. Louis, MO, and approved January 10, 2000 (received for review August 17, 1999) Metrosideros subg. Metrosideros (Myrtaceae) comprises Ϸ26 The very small seeds of Metrosideros require wind speeds of species distributed widely across the Pacific basin. They occur on only 5–19 km per h to be lofted. The seeds can retain viability the ancient Gondwanan landmasses of New Zealand and New in temperatures of Ϫ30°C for at least 6 h and after seawater Caledonia, as well as on the volcanic islands of the remote immersion for more than 1 month (10). Carlquist (1) suggested Pacific, from Melanesia to tropical Polynesia and the Bonin that the more remote taxa had achieved long-distance dispersal Island. Phylogenetic analysis based on nuclear ribosomal DNA to the isolated islands of Oceania on high-altitude jet streams spacer sequences from all named species showed Metrosideros that traverse the tropical Pacific from west to east. The most umbellata of New Zealand as basal in the subgenus, with the recent speculation on the dispersal history of subg. Metrosideros remaining species falling into three monophyletic clades. One (6) points to New Zealand rather than New Caledonia as the includes the seven New Caledonian species together with three landmass of origin and suggests that Samoa is the secondary axis daughters in western Oceania that probably dispersed during of dispersal into remote Polynesia.
    [Show full text]
  • Likely to Have Habitat Within Iras That ALLOW Road
    Item 3a - Sensitive Species National Master List By Region and Species Group Not likely to have habitat within IRAs Not likely to have Federal Likely to have habitat that DO NOT ALLOW habitat within IRAs Candidate within IRAs that DO Likely to have habitat road (re)construction that ALLOW road Forest Service Species Under NOT ALLOW road within IRAs that ALLOW but could be (re)construction but Species Scientific Name Common Name Species Group Region ESA (re)construction? road (re)construction? affected? could be affected? Bufo boreas boreas Boreal Western Toad Amphibian 1 No Yes Yes No No Plethodon vandykei idahoensis Coeur D'Alene Salamander Amphibian 1 No Yes Yes No No Rana pipiens Northern Leopard Frog Amphibian 1 No Yes Yes No No Accipiter gentilis Northern Goshawk Bird 1 No Yes Yes No No Ammodramus bairdii Baird's Sparrow Bird 1 No No Yes No No Anthus spragueii Sprague's Pipit Bird 1 No No Yes No No Centrocercus urophasianus Sage Grouse Bird 1 No Yes Yes No No Cygnus buccinator Trumpeter Swan Bird 1 No Yes Yes No No Falco peregrinus anatum American Peregrine Falcon Bird 1 No Yes Yes No No Gavia immer Common Loon Bird 1 No Yes Yes No No Histrionicus histrionicus Harlequin Duck Bird 1 No Yes Yes No No Lanius ludovicianus Loggerhead Shrike Bird 1 No Yes Yes No No Oreortyx pictus Mountain Quail Bird 1 No Yes Yes No No Otus flammeolus Flammulated Owl Bird 1 No Yes Yes No No Picoides albolarvatus White-Headed Woodpecker Bird 1 No Yes Yes No No Picoides arcticus Black-Backed Woodpecker Bird 1 No Yes Yes No No Speotyto cunicularia Burrowing
    [Show full text]
  • Seed Ecology Iii
    SEED ECOLOGY III The Third International Society for Seed Science Meeting on Seeds and the Environment “Seeds and Change” Conference Proceedings June 20 to June 24, 2010 Salt Lake City, Utah, USA Editors: R. Pendleton, S. Meyer, B. Schultz Proceedings of the Seed Ecology III Conference Preface Extended abstracts included in this proceedings will be made available online. Enquiries and requests for hardcopies of this volume should be sent to: Dr. Rosemary Pendleton USFS Rocky Mountain Research Station Albuquerque Forestry Sciences Laboratory 333 Broadway SE Suite 115 Albuquerque, New Mexico, USA 87102-3497 The extended abstracts in this proceedings were edited for clarity. Seed Ecology III logo designed by Bitsy Schultz. i June 2010, Salt Lake City, Utah Proceedings of the Seed Ecology III Conference Table of Contents Germination Ecology of Dry Sandy Grassland Species along a pH-Gradient Simulated by Different Aluminium Concentrations.....................................................................................................................1 M Abedi, M Bartelheimer, Ralph Krall and Peter Poschlod Induction and Release of Secondary Dormancy under Field Conditions in Bromus tectorum.......................2 PS Allen, SE Meyer, and K Foote Seedling Production for Purposes of Biodiversity Restoration in the Brazilian Cerrado Region Can Be Greatly Enhanced by Seed Pretreatments Derived from Seed Technology......................................................4 S Anese, GCM Soares, ACB Matos, DAB Pinto, EAA da Silva, and HWM Hilhorst
    [Show full text]
  • Volume 73, Number
    New Zealand Science Review Vol 73 (3–4) 2016 Symposium on Systematics and Biodiversity in honour of Dr Dennis P. Gordon Official Journal of the New Zealand Association of Scientists ISSN 0028-8667 New Zealand Science Review Vol 73 (3–4) 2016 Official Journal of the New Zealand Association of Scientists P O Box 1874, Wellington www.scientists.org.nz A forum for the exchange of views on science and science policy Managing Editor: Allen Petrey Contents Guest Editor: Daniel Leduc Production Editor: Geoff Gregory Editorial .....................................................................................................................................................61 Proceedings of a Symposium on Systematics and Biodiversity: Past, Present and Future, National Institute of Water & Atmospheric Research, Wellington, April 2016 Bryozoa—not a minor phylum – Dennis P. Gordon and Mark J. Costello ..................................................63 The contribution of Dennis P. Gordon to the understanding of New Zealand Bryozoa – Abigail M Smith, Philip Bock and Peter Batson ................................................................................67 The study of taxonomy and systematics enhances ecological and conservation science – Ashley A. Rowden ............................................................................................................................72 Taxonomic research, collections and associated databases – and the changing science scene in New Zealand – Wendy Nelson .............................................................................79
    [Show full text]
  • Christ's Indian Paintbrush (Castilleja Christii) Monitoring on the Sawtooth
    CHRIST’S INDIAN PAINTBRUSH (CASTILLEJA CHRISTII) MONITORING ON THE SAWTOOTH NATIONAL FOREST: SECOND-YEAR RESULTS by Robert K. Moseley Conservation Data Center March 1997 Idaho Department of Fish and Game 600 South Walnut, P.O. Box 25 Boise, ID 83707 Stephen P. Mealey, Director Sawtooth National Forest Idaho Department of Fish and Game Agreement No. 14-CCS-96-023 ABSTRACT Christ’s Indian paintbrush (Castilleja christii) is Idaho’s rarest plant, consisting of a single population on Mount Harrison at the north end of the Albion Mountains, Cassia County. Due to its extreme rarity and the numerous disturbances that take place on the summit plateau of Mount Harrison, a Conservation Agreement was signed between the Sawtooth National Forest and the U.S. Fish and Wildlife Service that enumerates conservation actions that will be implemented to protect habitat for Christ’s Indian paintbrush, including establishing a monitoring program to assess impacts to the population associated with recreational uses. The monitoring program was established by the Idaho Department of Fish and Game’s Conservation Data Center in 1995 (Moseley 1996). The objectives for 1996 were to: (1) continue to collect density and frequency data for Christ’s Indian paintbrush at the 20 transects established in 1995 and (2) establish monitoring of habitat recovery where a buried electronic cable was laid through a small portion of paintbrush habitat in late 1995. Results of the population monitoring show that the total number of plants and stems increased in 1996, but in only one transect was that increase statistically significant for plant density. Three transects showed significant increases in stem production.
    [Show full text]
  • Voorraad 25-2-2020
    Voorraad 25 Voorraad boskoop.nl - boskoop.nl boskoop.nl - - - 2 - 2020 Rijneveld Rijneveld 115 2771 XV Boskoop www.bulk info@bulk anne@bulk Fax.: +31 (0) 172 213 402 Prijzen onder voorbehoud van fouten Prices subject to errors Handelvoorwaarden op onze website - conditions of sale on our website - Handelsbedingungen auf unsere Website ------------------------------------------------------------------------------------- - Planten kunnen worden besteld en opgahald. We kunnen helaas geen postpaketten meer versturen - Plants can be preordered and picked up. Unfortunately we cannot send any postparcels anymore Aantal Naam MvL Maat cm Prijs Quantety Name Rootball/pot. Hight cm kluit/pot 22 Abelia triflora 25-30 C2 € 19,70 124 Abies koreana 20/+ C2 € 11,25 13 Abies numidica 30-40 C3 € 22,50 5 Acaena magellanica plgd P9 € 5,00 1 Acer aff. davidii 50-60 C2 € 16,50 1 Acer aff. palmatum 60-80 KL € 21,85 1 Acer 'Ample Surprise' PBR 100-125 C25 € 75,00 2 Acer 'Ample Surprise' PBR 20-30 C3 € 27,50 1 Acer 'Ample Surprise' PBR 20-30 C3 € 27,50 3 Acer amplum 225-250 KL € 59,50 1 Acer campestre 'Huiber's Elegant' 160-180 C5 € 39,50 1 Acer cappadocicum 'Aureum' 140-160 C12 € 49,50 21 Acer cappadocicum 'Aureum' 40-60 P11 € 10,95 1 Acer carpinifolium SOL C100 € 412,50 2 Acer conspicuum 'Red Flamingo' 150-175 C7.5 € 52,00 1 Acer davidii 100-125 C3 € 18,75 1 Acer davidii 80-100 C10 € 19,95 2 Acer davidii 120-150 C5 € 30,00 30 Acer davidii ('Serpentine'-seedling) 80-120 C2 € 17,50 2 Acer davidii 'George Forrest' 100-125 C2 € 26,90 5 Acer davidii 'George Forrest'
    [Show full text]
  • University of Florida Thesis Or Dissertation Formatting
    SYSTEMATICS OF TRIBE TRICHOCEREEAE AND POPULATION GENETICS OF Haageocereus (CACTACEAE) By MÓNICA ARAKAKI MAKISHI A DISSERTATION PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY UNIVERSITY OF FLORIDA 2008 1 © 2008 Mónica Arakaki Makishi 2 To my parents, Bunzo and Cristina, and to my sisters and brother. 3 ACKNOWLEDGMENTS I want to express my deepest appreciation to my advisors, Douglas Soltis and Pamela Soltis, for their consistent support, encouragement and generosity of time. I would also like to thank Norris Williams and Michael Miyamoto, members of my committee, for their guidance, good disposition and positive feedback. Special thanks go to Carlos Ostolaza and Fátima Cáceres, for sharing their knowledge on Peruvian Cactaceae, and for providing essential plant material, confirmation of identifications, and their detailed observations of cacti in the field. I am indebted to the many individuals that have directly or indirectly supported me during the fieldwork: Carlos Ostolaza, Fátima Cáceres, Asunción Cano, Blanca León, José Roque, María La Torre, Richard Aguilar, Nestor Cieza, Olivier Klopfenstein, Martha Vargas, Natalia Calderón, Freddy Peláez, Yammil Ramírez, Eric Rodríguez, Percy Sandoval, and Kenneth Young (Peru); Stephan Beck, Noemí Quispe, Lorena Rey, Rosa Meneses, Alejandro Apaza, Esther Valenzuela, Mónica Zeballos, Freddy Centeno, Alfredo Fuentes, and Ramiro Lopez (Bolivia); María E. Ramírez, Mélica Muñoz, and Raquel Pinto (Chile). I thank the curators and staff of the herbaria B, F, FLAS, LPB, MO, USM, U, TEX, UNSA and ZSS, who kindly loaned specimens or made information available through electronic means. Thanks to Carlos Ostolaza for providing seeds of Haageocereus tenuis, to Graham Charles for seeds of Blossfeldia sucrensis and Acanthocalycium spiniflorum, to Donald Henne for specimens of Haageocereus lanugispinus; and to Bernard Hauser and Kent Vliet for aid with microscopy.
    [Show full text]
  • United States of America
    anran Forestry Department Food and Agriculture Organization of the United Nations GLOBAL FOREST RESOURCES ASSESSMENT COUNTRY REPORTS NITED TATES OF MERICA U S A FRA2005/040 Rome, 2005 FRA 2005 – Country Report 040 UNITED STATES OF AMERICA The Forest Resources Assessment Programme Sustainably managed forests have multiple environmental and socio-economic functions important at the global, national and local scales, and play a vital part in sustainable development. Reliable and up- to-date information on the state of forest resources - not only on area and area change, but also on such variables as growing stock, wood and non-wood products, carbon, protected areas, use of forests for recreation and other services, biological diversity and forests’ contribution to national economies - is crucial to support decision-making for policies and programmes in forestry and sustainable development at all levels. FAO, at the request of its member countries, regularly monitors the world’s forests and their management and uses through the Forest Resources Assessment Programme. This country report forms part of the Global Forest Resources Assessment 2005 (FRA 2005), which is the most comprehensive assessment to date. More than 800 people have been involved, including 172 national correspondents and their colleagues, an Advisory Group, international experts, FAO staff, consultants and volunteers. Information has been collated from 229 countries and territories for three points in time: 1990, 2000 and 2005. The reporting framework for FRA 2005 is based on the thematic elements of sustainable forest management acknowledged in intergovernmental forest-related fora and includes more than 40 variables related to the extent, condition, uses and values of forest resources.
    [Show full text]
  • Tmcm1de1.Pdf
    Departament de Biologia Facultat de Ciències Hybridization patterns in Balearic endemic plants assessed by molecular and morphological markers — Ph. D. Thesis — Miquel Àngel Conesa Muñoz Supervisors: Dr. Maurici Mus Amézquita (Universitat de les Illes Balears) Dr. Josep Antoni Rosselló Picornell (Universitat de València) May 2010 Palma de Mallorca El doctor Maurici Mus Amézquita, professor titular de la Universitat de les Illes Balears, i el doctor Josep Antoni Rosselló Picornell, professor titular de la Universitat de València, CERTIFIQUEN: Que D. Miquel Àngel Conesa Muñoz ha realitzat, baix la seva direcció en el Laboratori de Botànica de la Universitat de les Illes Balears i en el Departament de Botànica del Jardí Botànic de la Universitat de València, el treball per optar al grau de Doctor en Biologia de les Plantes en Condicions Mediterrànies, amb el títol: “HYBRIDIZATION PATTERNS IN BALEARIC ENDEMIC PLANTS ASSESSED BY MOLECULAR AND MORPHOLOGICAL MARKERS” Considerant finalitzada la present memòria, autoritzem la seva presentació amb la finalitat de ser jutjada pel tribunal corresponent. I per tal que així consti, signem el present certificat a Palma de Mallorca, a 27 de maig de 2010. Dr. Maurici Mus Dr. Josep A. Rosselló 1 2 A la meva família, als meus pares. 3 4 Agraïments - Acknowledgements En la vida tot arriba. A moments semblava que no seria així, però aquesta tesi també s’ha acabat. Per arribar avui a escriure aquestes línies, moltes persones han patit amb mi, per mi, o m’han aportat el seu coneixement i part del seu temps. Així doncs, merescut és que els recordi aquí. Segurament deixaré algú, que recordaré quan ja sigui massa tard per incloure’l.
    [Show full text]