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TOYOPEARL AF-Rprotein a HC-650F Best-In-Class Bioprocessing Resins TOYOPEARL® AF-rProtein A HC-650F TOYOPEARL AF-rProtein L-650F TOYOPEARL Sulfate-650F TOYOPEARL NH2-750F Experts in Chromatography Solutions for Your Purification and Separation Tasks from Lab to Production Tosoh Bioscience is a leading supplier of chromatographic media, columns and gel permeation chromatography (GPC) instruments with over 500 specialty products to meet your analysis and purification needs. TOYOPEARL, TSKgel® & Ca++Pure-HA™ Resins TOYOPEARL and TSKgel chromatography resins are specifically designed for the purification of biomolecules. Ca++Pure-HA is a hydroxyapatite resin and has unique separation properties for biomolecules. These resins show excellent physical strength and ideal flow characteristics for industrial downstream processing. TSKgel U/HPLC Columns Extensively used in laboratories all over the world, our TSKgel columns are designed for researchers seeking the highest level of performance. Covering the total range of U/HPLC, these columns offer high resolution, excellent reproducibility and long column life. Scaling up from analytical to preparative columns is made simple and easy. EcoSEC® GPC Systems The EcoSEC series of fully automated liquid chromatography systems for gel permeation chromatography is designed for robust polymer analysis. Both solutions, for ambient and for high temperature GPC, combine dual pump solvent systems, sophisticated heating and a highly efficient detection system to deliver the highest reproducibility. In this brochure, four best-in-class TOYOPEARL resins are introduced. These resins are designed for mAb purification with exceptionally high capacities and excellent impurity removal properties. These features provide process developers with greater flexibility of operational parameters, leading to higher productivity. TOYOPEARL Resins TOYOPEARL AF-rProtein A HC-650F The Best-In-Class Resin: an economical protein A resin with the highest dynamic binding capacity TOYOPEARL AF-rProtein L-650F Protein A chromatography is the most widely used type of Table 1. Characteristics of TOYOPEARL AF-rProtein A HC-650F resin affinity chromatography. Its high specificity for the Fc region of antibodies is the primary reason for its dominance as the Particle size 45 µm affinityTOYOPEARL ligand of choice in antibody Sulfate-650F purification applications. Increasing usage of monoclonal antibodies (mAbs) as Pore diameter 100 nm biological drugs in management of chronic diseases has DBC (5 min) 70 g/L driven the increased demand of protein A resins. DBC (2 min) 50 g/L With pressuresTOYOPEARL mounting to reduce NH production2-750F costs at many Caustic stability > 200 CIP cycles (0.1 mol/L NaOH) companies, and protein A being the most expensive resin Max. pressure 0.3 MPa used in mAb purification, the use of a high capacity protein A resin can significantly impact the overall cost of doing High dynamic binding capacity business. To reduce the production cost at many companies, scientists are often looking for high binding capacity protein The results of a dynamic binding capacity A resins with high recovery at various flow rates. (DBC) comparison study between TOYOPEARL AF-rProtein A HC-650F and another commercially TOYOPEARL AF-rProtein A HC-650F is a hydroxylated available protein A resin is detailed in Table 2. The data methacrylic polymer high capacity protein A resin for shows that TOYOPEARL AF-rProtein A HC-650F has much monoclonal antibody purification. Table 1 lists the higher DBC. Indeed, at 2 minutes residence time, this properties and dynamic binding capacities of this resin. resin is able to absorb as much as 50 g IgG/L resin and at 5 minutes residence time, TOYOPEARL AF-rProtein A HC-650F TOYOPEARL AF-rProtein A HC-650F resin offers: resin’s dynamic binding capacity reaches 70 g/L. This is • High binding capacity – up to 70 g/L, the highest almost 20% higher than the dynamic binding capacity of a in its class competitive resin. • Reduced operating cost – use of shorter columns Table 2. Dynamic binding capacity of TOYOPEARL AF-rProtein A HC-650F requires less resin resin and competitor • Superior recovery – up to 90% at 65 g/L load DBC-2 min DBC-5 min Product name SupplierBead diameter (g/L) (g/L) • High alkaline resistance – ensures long operational TOYOPEARL Tosoh lifetime and improves process economics 45 µm 50 70 AF-rProtein A HC-650F Bioscience • Excellent pressure-flow stability – low pressure drop Competitive Product Competitor X85 µm 30 58 allows high flexibility of operational parameters • Lowest ligand leaching – allows long term usage of resin before its effective operational life is complete • High affinity to more subclasses of mAbs – the best-in-class and most cost-effective resin for capturing mAbs from hybridoma, mouse and CHO cell lines For more info visit: www.tosohbioscience.com 1 TOYOPEARL Resins Reduced operating cost With its high dynamic binding capacity, TOYOPEARL AF-rProtein A HC-650F resin does not have to be packed in the typical column bed height range of 20 cm. Use of a shorter column requires less resin. As can be seen from the below comparisons in Table 3, making use of a high capacity protein A resin in your purification process is an excellent way to save on production costs. Table 3. Production cost comparisons using TOYOPEARL AF-rProtein A HC-650F resin and competitor Column Column Resin volume Packed DBC at Column Packed bed Resin Resin cost Residence Resin diameter volume to pack column residence capacity height (cm) compression per liter time (min) (cm) (L) column cost time (g/L) 80% DBC (g) TOYOPEARL AF-rProtein A HC-650F 15 36 15 1.25 20 $12,000 $240,000 60 732 3 Resin X 21 40 26 1.16 31 $12,000 $372,000 35 739 3 Superior recovery Figure 1. Stability of TOYOPEARL AF-rProtein A HC-650F resin TOYOPEARL AF-rProtein A HC-650F is capable of delivering high purity monoclonal antibodies with excellent recovery 120 at loading levels approaching the resin capacity without any increase in aggregate levels (Table 4). This level of 100 performance will allow chromatographers to fully utilize the increased capacity of this resin without compromising 80 quality while at the same time increasing the productivity of the antibody capture step. 60 Table 4. Load and recovery levels of TOYOPEARL AF-rProtein A HC-650F resin 40 DBC at 10% breakthrough (%) Load % Monomer % Recovery 20 35 g/L 96.1 87.2 0 50 g/L 96.8 86.5 020406080 100 120 140 160 180 200 220 240 260 280 300 65 g/L 96.1 89.5 Number of CIP cycles High alkaline resistance Figure 2. Pressure-flow of TOYOPEARL AF-rProtein A HC-650F resin TOYOPEARL AF-rProtein A HC-650F resin was subjected to 0.3 300 purification cycles. Clean-in-place (CIP) was done with Column pressure 0.2 mol/L NaOH following each cycle, with a contact time 0.25 of 15 minutes. As can be seen from Figure 1, the capacity, normalized to 100% prior to the first CIP cycle, remains 0.2 virtually unchanged for approximately 200 cycles. This data indicates that the TOYOPEARL AF-rProtein A HC-650F resin 0.15 is exceptionally stable in NaOH, ensuring a long operational Pressure (MPa) lifetime, thereby improving process economics. 0.1 Excellent pressure-flow stability 0.05 TOYOPEARL AF-rProtein A HC-650F resin is based 0 on a hydrophilic, dimensionally stable base resin, 0 100 200 300 400 500 600 Linear velocity (cm/hr) TOYOPEARL HW-65, that exhibits excellent pressure-flow characteristics (see Figure 2). The 100 nm pore diameter Resin: TOYOPEARL AF-rProtein A HC-650F Column type: GE Healthcare AxiChrom of the TOYOPEARL affinity resins can accommodate large Column size: 20 cm ID × 10 cm (3,140 mL) globular proteins up to 5 × 106. The semi-rigid TOYOPEARL Mobile phase: DI water Flow rate: various AF-rProtein A HC-650F beads do not distort under flow rates that generate up to 0.3 MPa pressure. Call customer service: 866-527-3587, 2 technical service: 800-366-4875, option #3 TOYOPEARL Resins Lowest ligand leaching High affinity to more subclasses of mAbs Figure 3 below shows the amount of ligand eluted from the Because the recombinant protein A ligand of the TOYOPEARL AF-rProtein A HC-650F resin over 200 cycles TOYOPEARL AF-rProtein A HC-650F resin is a code-modified using 0.2 mol/L NaOH to clean in place (CIP) between each hexamer of the C domain, this resin has an affinity for cycle. As the number of CIP cycles increased, the amount various antibodies that the native protein A and some other of ligand present in the eluted product decreased. recombinant protein A ligands do not possess. For example, This indicates that the TOYOPEARL AF-rProtein A it has high affinity for different subclasses of antibodies HC-650F resin has a very stable ligand attachment and from rat and goat which native protein A does not have any meets the performance expectations required in the affinity for, as demonstrated in Table 5. biopharmaceutical industry for ligand leaching. This also demonstrates the potential for long term use of TOYOPEARL Table 5. TOYOPEARL AF-rProtein A HC-650F ligand with a broad affinity AF-rProtein A HC-650F resin before its effective operational range for mAb subclasses life is complete. rProtein A ligand Native Protein A Figure 3. Low ligand leaching of TOYOPEARL AF-rProtein A HC-650F resin SpeciesSubclass (TOYOPEARL Ligand AF- rProtein A HC-650F) 6 HybridomaIgG +++++++ IgG1 ++++ + 5 IgG +++++ ++++ Mouse 2a IgG2b +++++ +++ 4 IgG3 ++++ ++ IgG1 +++++ ++++ 3 Human IgG2 +++++ ++++ (CHO) IgG3 - - IgG +++++ ++++ 2 4 Protein A leaching (ng/mL) IgG1 ++++ - IgG - - 1 Rat 2a IgG2b +++ - IgG2c ++++ - 0 020406080 100 120 140 160 180 200 Goat IgGs ++++ - Number of CIP cycles Rabbit IgG+++++ ++++ Ordering Information Part# Description 23425 TOYOPEARL AF-rProtein A HC-650F, 10 mL, 45 µm 23426 TOYOPEARL AF-rProtein A HC-650F, 25 mL, 45 µm 23427 TOYOPEARL AF-rProtein A HC-650F,100 mL, 45 µm 23428 TOYOPEARL AF-rProtein A HC-650F, 1 L, 45 µm 23429 TOYOPEARL AF-rProtein A HC-650F, 5 L, 45 µm 45201 SkillPak™ TOYOPEARL AF-rProtein A HC-650F 1 mL column 45222 SkillPak TOYOPEARL AF-rProtein A HC-650F 1 mL columns (qty.
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