rd 3 International conference

Oran 05-07 December

BIOSCIENCES 2019

International Conference on Biotechnology and Cancer (ICBC)

Oran, Algeria, December 07-08, 2019

Dear Conference participant,

It is with the greatest pleasure that I welcome you to the International Conference on Biotechnology and Cancer (ICBC).

ICBC is the third international conference "Biosciences19", it is a part of the “Biosciences” conference series initiated by the Higher School of Biological Sciences of Oran. Ever since its foundation, “Biosciences" conference has attracted numerous Algerian and international researchers interested in biotechnology advances. After shedding light on " Biology professions" during the first conference in 2017, and the "Enzymatic Engineering and Production of Metabolites" during de second version last year, Biosciences conference is focusing this year on another interesting and hotly debated subject which is “Biotechnology and Cancer”. The latter has raised endless questions over the last decade and is now a strategic field that occupies a privileged position in research system from all over the world. Major and ongoing advances in cancer research have greatly contributed to cancer diagnosis and development of new oncotherapy approaches including biotechnology and gene engineering which have considerably increased the cure rate and are improving life expectancy for incurable diseases. The 3rd Biosciences Conference, is an opportunity to bring under the same roof biologists, practitioners as well as health professionals from diverse backgrounds. All talks and presentations planned will be illustrating and discussing innovative research. Workshops and debates are also scheduled to encourage knowledge sharing and to explore achievements in the field of cancer biotechnology.

Pr .Djamel Saidi,

Head of the Higher School of Biological Sciences of Oran (HSBSO).

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Under the high patronage of the Minister of Higher Education and Scientific Research

President of the scientific committee Pr SAIDI Djamel President of the organizing committee Pr BABA HAMED Mohamed Bey Scientific Committee FOUATIH Zoubir Université Oran 1 BOUSAHBA Abdelkader Université Oran 1 BITAM Idir ESSAIA, Alger BOUSAHBA Abdelkader Université Oran 1, Algeria MERGHOUB Taha New York City, USA CHENNA Ahmad San Francisco, USA HIHI Abdelmadjid Clinatec, Grenoble, France BOUDJELAL Mohamed Riyadh. KSA BEREKSI REGUIG Faiza CHU Hassani Abdelkader de Sidi Bel Abbes BEREKSI REGUIG FAIZA Chef de service oncologie, Sidi Bel Abbes ABERKANE Meryem USTO, Oran, Algeria MESLI Farida Université Oran 1, Algeria FELIDJ Menel ESSB- Oran RAHLI Fouzia ESSB- Oran BOUGHRARA Wefa ESSB- Oran MERZOUG Mohamed ESSB- Oran MAHAMMI Fatima Zohra ESSB- Oran CHOUBANE Slimane ESSB- Oran SADDIKIOUI Leila ESSB- Oran BOUKHARI BENAHMED DAIDJ Nabila ESSB- Oran BEREKSI Samia ESSB- Oran MAHMOUDI Bahia ESSB- Oran YAKOUBI Fatima ESSB- Oran BENAYAD Sarah ESSB- Oran BENYETTOU Imene ESSB- Oran KHELIL Omar ESSB- Oran MAHDJOUR Soumicha ESSB- Oran ILIAS-CHERIF Wassila ESSB- Oran Organizing Committee FOUATIH Zoubir Université Oran 1 BOUSAHBA Abdelkader Université Oran 1 BEREKSI REGUIG Faiza CHU Hassani Abdelkader de Sidi Bel Abbes FELIDJ Menel ESSB- Oran RAHLI Fouzia ESSB- Oran BOUGHRARA Wefa ESSB- Oran MERZOUG Mohamed ESSB- Oran MAHAMMI Fatima Zohra ESSB- Oran CHOUBANE Slimane ESSB- Oran SADDIKIOUI Leila ESSB- Oran MAHMOUDI Bahia ESSB- Oran BEREKSI Samia ESSB- Oran

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YAKOUBI Fatima ESSB- Oran BOUKHARI BENAHMED DAIDJ Nabila ESSB- Oran BOUKADOUM Ali ESSB- Oran REDOUANE Dalal ESSB- Oran BENAYAD Sarah ESSB- Oran BENYETTOU Imene ESSB- Oran KHELIL Omar ESSB- Oran MAHDJOUR Soumicha ESSB- Oran ILIAS-CHERIF Wassila ESSB- Oran AGGAB Tayeb ESSB- Oran SAAD AZZOUZ Karima ESSB- Oran SOUAK Abdelkader ESSB- Oran MAHIDA Rachid ESSB- Oran YACOUBI Lamia ESSB- Oran

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Oran 05-07 December

Table of contents

Coference program 15 Full name and title

Plenary session 18 Zoubir Ahmed Fouatih ([email protected]) 19 Apport du Réseau National des Registres du Cancer au Plan National Cancer 2015 -2019 Jacques Robert ([email protected]) 19 Oncogenèse et progression des cancers Abdelkader Boussahba ([email protected]) 20 Actualités sur l'immunothérapie dans le cancer bronchique Faiza Bereksi-Reguig ([email protected]) 20 Tumeurs neuroendocrines Miloud Medjamia ([email protected]) Rôle du pathologiste dans les testings moléculaires en cancérologie et expérience du service de 21 Pathologie de l'hôpital militaire régional universitaire d’Oran Idir Bitam ([email protected]) 21 Apport des nouvelles technologies en Oncologie Ammar Azioune 22 Les biotechnologies et l’écosystème national Abdallah Boudjemaa ([email protected]) 22 Pharmacogénétique et cancers: études algériennes Sami Djoulah ([email protected]) 23 Bioengineering/IA et Oncologie, des métiers d’avenir pour les biologistes Dominique Charron ([email protected]) 23 HLA & cancer à l'ère de la médecine de précision Taha Merghoub ([email protected]) 24 Develop means to overcome resistance to immune checkpoint blockade Mourad Aribi 24 Tumor antigen cell lysate-pulsed classically activated M1 macrophages for vaccination against cancer Abdelmadjid Hihi ([email protected]) Ecosystèmes de recherche translationnelle : un modèle pour accélérer la R&D du laboratoire à l'usage 25 médical Ali Bouamrani ([email protected]) 25 Brainprint: du concept clinique à la création d'une start-up Noureddine Sadeg ([email protected]) 26 Application de la nanotechnologie à la biologie et la médecine : nouvelles perspectives Hafid Belhadj Tahar ([email protected]) Conception et validations préclinique et clinique d'un nouvel agent anticancéreux issu de la 26 nanotechnologie "Imdendrim" Oral session 27 Oncogenomics and Pharmacogenomics 28 Asma Mimouni ([email protected]) 29 Association of CHRNA5 rs16969968 Polymorphism and Predisposition to Lung Cancer in Algeria Fatima Zohra Moghtit ([email protected]) 29 The frequency of EGFR mutations in lung carcinoma in the west Algerian population Tifrit Abdelkrim ([email protected]) 30 Spectacular advances in genomics in favor of the implementation of Pharmacogenomics Djeda Belharazem ([email protected]) 30 New cancer therapy strategy by targeting rescue molecules and dormancy

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Cancer Epigenetics and Nanotechnology in cancer treatment 32 Djefal Assia ([email protected]) 33 Development of Transplantable Tumoral Graft in Immune-compromised Wistar Rats Amine El-Mokhtar Drici ([email protected]) Genetic and epigenetic profile of gastric carcinoma infected with Helicobacter pylori in an Algerian 33 population Messal Ahlem Nora ([email protected]) 34 New biological dosimetry tool for detecting radio-induced aberrations Abdelkader Rahmouni ([email protected]) New design for synthesis of anti tumor nano heterocyclic molecules called frog, fox and bat catalyzed by 35 maghnite (Algerian MMT) Nadia Taibi ([email protected]) Metabolic Treatment against Cancer Methylglyoxal, a new target in the hepatocarcinoma treatment: An 35 alternative approach for coumarin drug carriers Novel approaches In cancer therapies 37 Felidj Menel ([email protected]) 38 Herbal medicinal plants using by cancer patients at ancology departementof CHU Blida, Algeria Kambouche Nadia ([email protected]) 38 Study of antioxidant effect from Algerian medicinal plants Dehimat Abdelouahab ([email protected]) Cytotoxicity, Anticancer Dose-time manner and Apoptotic Activity in Human Breast Cancer Cells by 39 Natural products Derived from Medicinal Herbs Extracts Bouhenna Mustapha Mounir ([email protected]) Real-time monitoring by electrical impedance spectroscopy of cell viability during treatment with 40 Chromones and Coumarins derivatives Houria Lamari-Negache ([email protected]) Radioprotective effect of Rosemary crude extract (Rosmarinus officinalis L.) against radiation induced 40 DNA damage on Vero cells Meriem Ladli ([email protected]) The use of direct AMPK activators as a therapeutic treatment could induce the apoptosis of mature 41 erythroblasts in the bone marrow Bachir Benarba ([email protected]) Anti-breast cancer activity, apoptosis induction, antioxidant potential and anti-inflammatory effects of 42 Bryonia dioica growing in Algeria Zergoug Ismail ([email protected]) 42 IMRT/VMAT as innovative technique in the treatment of cancer in radiation therapy Leïla Sarah Sellam ([email protected]) 43 Silibinin suppresses hypoxia induced aerobic glycolysis in nasopharyngeal carcinoma Moufida Mahdjour ([email protected]) 43 Exosomes for drug delivery to lung cancer Louisa Béchohra ([email protected]) DNA damage and mitochondria-mediated apoptosis of colorectal carcinoma cells induced by scorpion 44 venom components Sonia Seddiki ([email protected]) 45 Pathological and epidemiological features of ovarian carcinoma (serous / mucinous) in western Algeria Sarah Bouameur ([email protected]) 45 Nanoparticulate Drug Delivery System for Chemotherapy Sana Ferroudj ([email protected]) 46 Role of BRCA1 gene in hepatocellular carcinoma chemoresistance Soumicha Mahdjour ([email protected]) 46 In vitro anticancer activity of Ephedra vulgaris against different human cancer cell lines Cancer diagnostic technologies, Immunotechnology and Cancer Immunotherapy 48 Assia Galleze ([email protected]) 49 The use of Comet Assay as a valuable tool for assessing DNA damage in Algerian children with lymphoma

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Moustafa Sellah ([email protected]) Molecular interactions between anticancer drugs and their target cells and their risk against healthy 49 cells, cases of leukaemia and colorectal cancer in patients in the departments of oncology and hematoclinic at the University Hospital Centre in Tlemcen, Algeria Maamar Boukabcha ([email protected]) 50 The role of mammography, incidence and risk factors at patients with breast cancer Hassina Khaldoun ([email protected]) 51 Urinary tract infection in Algerian patients with bladder cancer: Histopathology and etiology Asma Bourefis ([email protected]) 51 Expression Of Human Serum Tctp In Prostate Cancer Is Correlated With The Agressivity Of Cancer Noria Bouras ([email protected]) 52 Exploration of exon 2 of the KRAS gene in Non-Small Cell Lung cancer (NSCLC) Samir Derouiche ([email protected]) 52 Epidemiological Study and role of zinc and lead on Breast Cancer in El Oued (Algerian) Population Souad Zoudji ([email protected]) 53 Effect of glucose oxidase on the cytokine profile and cholesterol of monocytes in liver cancer Chahinez Amira Dahmani ([email protected]) Association study of copy number variants in CCL3L1 gene with risk of ankylosing spondylitis in a West 54 Algerian population Ibtissem Ouadi ([email protected]) 54 Laboratory testing requirements for diagnosis of multiple myeloma Poster session 56 Oncogenomics and Pharmacogenomics 57 Walid Hassene Hamri ([email protected]) Epidemiological and histopathological assessement of central nervous system cancers in a northern 58 african population: a restrospective study over seven years Adel Gouri ([email protected]) Polymorphism of the Cytidine Deaminase (CDA) Gene and Response to Nucleoside analogs-based 58 Chemotherapy: The Value of a Phenotypic Test Boughrara Wefa ([email protected]) The genetic association between rs861539 and prostate cancer in Algerian population: cases-controls 59 study Chahinaz Benkheira ([email protected]) Study of genetic association between polymorphism Insertion / deletion of the angiotensin converting 59 enzyme gene, the ACE gene and the ABO gene in breast cancer in the population of Tlemcen Djabaria Naima Meroufel ([email protected]) 60 Are there any other mutations of the RB1 gene in patients with retinoblastoma in Algeria? Nora Chouha ([email protected]) Synthesis and evaluation of 1H-benzo[d]imidazol-1-yl-3-(4-chlorophenoxy)propan-2-ol derivatives as 61 cytotoxic agents Merabet Nabila ([email protected]) Genetic polymorphisms implication of estrogen metabolising enzymes in breast cancer susceptibility: 61 ongoing study on east Algerian women Sihem Derbal ([email protected]) Cancer pharmacogenomics strategies: investigations of organo-iridium anticancer complexes reveal 62 novel mechanism of action Hallal Nouria ([email protected]) Wormwood polyphenol extract mediated remediation of mitochondrial DNA (mtDNA) oxidation and 63 neurotoxicity induced by mercury Cancer Epigenetics and Nanotechnology in cancer treatment 64 Badid Naima ([email protected]) Relationship between chemotherapy treatment on oxidative stress markers in Algerian colorectal cancer 65 patients –A case control Study- Naima Badid ([email protected]) 65 Clinical Interests of the study of oxidative stress in gastric cancer-A case control-study- Cherifi Yasmina ([email protected] ) 66 CD10 a prognostic and predictive potential marker of liver metastasis in colorectal cancer

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Houria Ouldcadi ([email protected]) 66 Colorectal Cancer and Oxidative Stress in the West of Algeria Boudoukhani Meriem ([email protected]) 67 Physicochemical Characterization of Magnetic Nanogels Based Natural Polymers Zohra Touala-Chaila ([email protected]) Contribution at the research of the association between the MTHFR gene polymorphism 677C>T and 67 bladder cancer occurrence in a sample of the population of Western Algeria. Sihem Derbal ([email protected]) 68 Cancer challenges and opportunities : the nano-bio interactions of nanomedicines nanomedicine Fatima Zohra Zeggai ([email protected]) Anti timoral nano molecules called bats: New process for synthesis namely caesarean reaction under 69 effect of maghnite (Algerian MMT) Bensaid Nour El Houda ([email protected]) 70 Toxicity of carbon nanotube used in cancer therapy Nabil Ounoughi ([email protected]) 70 The use of Gold nanoparticles for cancer radiotherapy Bachir Ouari ([email protected]) 71 SuperAntiferromagnetic Nanoparticules- based Hyperthemia for cancers Treatment Kheira Zanoune Dellali ([email protected]) 71 5-Fluorouracil loaded- Magnetic nanoparticles for Targeted Cancer Therapy Nada Benyakoub ([email protected]) 72 Nanotechnology in oncology : Therapeutic aspects, toxicological risks and perspectives Novel approaches In cancer therapies 74 Louahem Soumaya ([email protected]) Chemopreventive effect of luteolin on 1, 2-dimethylhydrazine induced experimental colon 75 carcinogenesis Houari Fatima Zohra ([email protected]) Phytochemical screening and evaluation of the antioxidant activity of the methanolic extract of Rubia 75 Tinctorum. Ali Haimoud Safia ([email protected]) 76 Protective effect of extracts of propolis on liver injury induced by cisplatin used in cancer therapy in mice Amel Medjdoub Tahir ([email protected]) 76 Effects of Polyphenols on Lymphocyte Proliferation and Telomere Length Nour elhouda Bekkar ([email protected]) Acute toxicity of the methanolic extract of a medicinal plant from western Algeria : Zizyphus lotus in 77 wistar rats Houari Benamar ([email protected]) 77 Medicinal plants from Algeria: A complementary and alternative therapy for cancer Mohamed Nadjib Boukhatem ([email protected]) In vitro Cytotoxicity and Ex ovo Anti-Angiogenic Activity of Lemongrass (Cymbopogan citratus) Essential 78 Oil against Breast Carcinoma Cell Line Allioua Meryem ([email protected]) 79 Oxidative Stress in Patients with Non-Hodgkinal Lymphoma Alim Yousra ([email protected]) 79 The oyster mushroom, an edible mushroom rich in antioxidants, to prevent cancer Mohamed Nadjib Boukhatem ([email protected]) In Vitro Antioxidant, Anti-inflammatory and Cytotoxic Potential of Thyme (Thymus vulgaris) Essential 80 Oil and its Related Terpenes on Tumor Cell Lines (Breast cancer and Melanoma) Fatima Fadel ([email protected]) 81 Study and development of anticancer molecules by means of simulation and molecular modeling Ben Amar Cheba ([email protected]) 81 Laser Biophotonics for Cancer Diagnosis and Therapy (An Overview) Fatima Zohra Hantour ([email protected]) Traditional medicinal plants used by breast cancer patients: results from a retrospective study in 82 Northern West Algeria Zahia Benmouna ([email protected]) 82 Anti-tumoral and anti-proliferative activities of bacteriocins from lactic acid bacteria

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Benahmed Fatiha ([email protected]) 83 In Vitro Antioxidant and Antiproliferative properties of aqueous extract of Pistacia atlantica Majda Dali-sahi ([email protected]) Optimization in modeling of molecules for inhibition of α-synuclein aggregation during multisystematous 84 atrophy (MSA) Mokhtari Aitkaki Samira ([email protected]) Application of virtual screening in the selection of new inhibitors of the Bcr-Abl fusion protein, 84 anticancer (drug candidates) Derouicha Matmour ([email protected]) 85 Anticancer Drug Design: Development of Tyrosine Kinase Inhibitors Derouicha Matmour ([email protected]) 86 In Silico Studies of AXL Kinase Inhibitors Fatima Zohra Chenni ([email protected]) 86 Does Argan Oil affect Nitroso Compounds Formation? Zitouni Hanane ([email protected]) 87 Traditional medicine and cancer in Oran: what about interactions? Emoke Pall ([email protected]) 87 Antioxidant and antiproliferative potential of Syringa vulgaris L. flowers ethanolic extract Hayet Messaoui ([email protected]) 88 Antioxidant activity of whey protein hydrolysates obtained by strains of Lactobacillus Nabila Taib ([email protected]) 88 Theoretical contribution of bastadins: molecules of therapeutic interest Dermeche Keltoum ([email protected]) 89 Emotional freedom techniques for cancer Nada Zabaiou ([email protected]) 89 Propolis extract reduces prostate cancer cells proliferation in Wistar rat. Dib Hanane ([email protected]) 90 Evaluation of protective effects of opuntia ficus-indica cladodes on colorectal cancer Krid Meryem ([email protected]) 91 Pharmacoeconomics and nanotherapy in cancer treatment Abdelhafid Nani ([email protected]) Polyphenols from Pennisetum glaucum grains induce caspase-mediated apoptosis in human 92 osteosarcoma cells Fekhikher Imene ([email protected]) Chimeric Antigen Receptor T-cell (CAR -T) Therapy for Hematologic and Solid Malignancies Risk and 92 benefits- A Systematic Review with Meta-Analysis Ikram chebiebe ([email protected]) 93 LRRK2 Protein Inhibitors During Parkinson's Pathology Mohammed Dellali ([email protected]) Synthesis and characterization of hydrogel films based on polysaccharide and their applications in the 94 treatment of skin cancer Cancer diagnostic technologies, Immunotechnology and Cancer Immunotherapy 95 Afaf Benhouda ([email protected]) 96 Association between clinicopathological characteristics and KRAS mutation in colorectal cancer Behar Dalale ([email protected]) Detection of papillomavirus (HPV) infections at the cervix in women at risk (HIV-positive) by a method 96 of molecular biology: classic pcr Nusyba Ahmed Bushra ([email protected]) Comparison Between Modified Papanicolaou Stain and Hematoxylin–Eosin Stain for The Demonstration 97 of Keratin in Oral Tissue Sections Meryem Saidi ([email protected]) 97 Instance selection algorithms for a Cost sensitive medical diagnosis Leila Saadi ([email protected]) 98 Interest of beta catenin in the diagnosis and targeted therapy of hepatocellular cancer Latifa Khattabi ([email protected]) Incidence and diagnosis of thyroid cancer in eastern Algeria: a retrospective study of 339 cases over 24 98 cities

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Billel Bouchikhi ([email protected]) 99 Monte Carlo simulation in mammographic imaging and evaluation of the dose distribution Imene Bendoukha ([email protected]) 100 Descriptive and Immunohistochemical Study of Lung Cancer Alaa Mohamed Foued Derdour ([email protected]) 10 Vibrational Spectroscopy – A diagnostic tool in oncology Yasmine Bekas ([email protected]) 101 Interest of anatomopathological examination in prostate cancer Amina Chami ([email protected]) Contribution of BRCA1 and BRCA2 Germline Mutations to Early Algerian Breast Cancer using High 101 Resolution Melting Analysis (HRM) Kerai Salim ([email protected]) 102 The Electrical Bioimpedance Technique in Cancer Diagnosis Asmahane Medjdoub ([email protected]) 102 Investigation of colorectal cancer via an anatomopathological approach Zineb Tahari ([email protected]) 103 Epidemiological and histopathological aspects of colorectal tumors in a West Algerian population Chenni Fatima Zohra ([email protected]) 103 Epidemiological and diagnostic aspects of 350 colorectal cancers cases in Sidi Bel Abbes (Algeria) Heyet Ramdani ([email protected]) 104 Primary Plasma Cell Leukemia: Immunochemical and Immunophenotypic Features Amal Mesli ([email protected]) Distribution study of G216T polymorphism of the EGFR gene and evaluation of its therapeutic prognosis 105 in Non-Small Cell Lung Carcinoma Mohammed Abdellaoui ([email protected]) Oral intake of aqueous extract of Graviola (Annoneceas Muricata) dried leaves suppresses the 7,12- 105 DMBA/Croton oil induced two-step skin carcinogenesis in Swiss albino mice Salah Eddine El Herrag ([email protected]) Tumor PD-L1 expression and Nivolumab efficacy in the treatment of cancer: a meta-analysis of phase III 106 randomized clinical trials Wassim Aymen Djihad Bouhaceine ([email protected]) Study of the possibility of using carcinoembryonic antigen 5 in the diagnosis of liver metastases and for 107 hepatocellular cancer Ihsan Alsaimary ([email protected]) 107 Occurrence of oral and oropharyngeal squamous cell carcinoma among patients in Basrah city Zahra Gabli ([email protected]) 108 Immunohistochemical markers of mammary tumors in female dogs from Northeastern Algeria Nora Kariche ([email protected]) Interleukin-17A and NOS2 signaling independently link NF-κB activity to epithelial to mesenchymal 108 transition in invasive laryngeal squamous cell carcinoma Yasmine Allal ([email protected]) Formulation of biomedicines for the treatment of metastatic colorectal cancers: what is the role of the 109 galenic pharmacist? Miliani Maroua ([email protected]) 109 Thymoquinone enhances IFN-γ-associated antitumor cytokine production Fizazi Anissa ([email protected]) 110 Role of Immuno-Oncology in Treating Cancer Biosciences 111 Mohamed Merzoug ([email protected]) Biochemical characterization and gene screening of bacteriocins produced by Enterococcus strains 112 isolated from dried figs fermented in olive oil Akli Islam Chebli ([email protected]) 112 Toxic Hazard Associated With Vaccination Nor el houda Benahmed ([email protected]) 113 Antioxidant profile of the polyphenol extract prepared by infusion of Juglans regia bark Mohamed Terki ([email protected]) 113 Research about the anioxydant activity of Citrullus colocynthis chloroformic exract

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Hafida Kazouz ([email protected]) 114 Antioxidative response to lead in Hordeum vulgare L. Zidi Ghania ([email protected]) 114 Improvement of antivenom immunotherapy by Alum nanoparticulate adjuvant Menaa Belkis ([email protected]) Isolation and characterization of endophytic actinobacteria associated with Artemisia sp from Batna 115 region Mohammed Aissaoui ([email protected]) 116 Phenolic extract, study of antimicrobial and antioxidant activities of Coffea arabica green seeds Nadia Aissaoui ([email protected]) Antistaphylococcic compound produced by thermophilic Bacillus licheniformis LMB3704 isolated from 116 the hot spring Dbagh in Algeria. Ali Tabeche ([email protected]) Contribution to the assessment of metallic pollution of the quality of marine waters by the use of two 117 bioindicators sole Solea solea (Linnaeus, 1758) and pageot acne Pagellus acarné (Risso, 1827) sins in the littoral oranais Hayat Djaara ([email protected]) Association between the C677T polymorphism of MTHFR, blood folate and vitamin B12 deficiency, and 118 elevated serum total homocysteine in young patients with myocardial infarction in Aures region (Algeria) Ati Moncef ([email protected]) A Computational Tool for Calculation of Absorbed Dose to Patients Following Bone Scan with 99m 119 Tc‐Marked Diphosphonates Dehimat Abdelouahab ([email protected]) Natural products to prevent oxidative stress and cancer: dose-manner response effect in human 119 leukemia cells (HL60) and cervical cancer cells (HeLa) Firdaous Fedoul ([email protected]) 120 Ethnobotanic Study Of Aloe vera In Western Algeria Nabila Hamidi ([email protected]) Oxidative stress and nutritional profile in an affected population of colorectal cancer in region of 120 Tlemcen Zineb Chellouai ([email protected]) 121 Mass spectrometry imaging an innovative tool in anticancer drug discovery Chenni Fatima Zohra ([email protected]) 121 Does Argan Oil affect Nitroso Compounds Formation? Mohammed Amine Belaouni ([email protected]) Screening of lipases, amylases, cellulases and proteases from thermophilic Pseudomonas and Bacillus 122 strains Samir Neggaz ([email protected]) 123 Effect of solvents on antibacterial activity of Tirmania nivea (Desf.) Trappe from Algeria Ouafia Liamani ([email protected]) 123 Use of FISH sperm technique for the study of aneuploidies in men with abnormal sperm parameters Khaled Touati ([email protected]) 124 Halophilic microalgae Dunaliella: biological resource of beta carotene for pharmacology Asfouri Nadia Yasmine ([email protected]) 124 Impact of cultivation conditions and media content on Nannochloropsis gradiation composition Hafsa Benaiche ([email protected]) 125 Ethnobotanic And Floristic Study Of Medicinal Plants Used In The Region Of Oran, Algeria Dahmani Bouchra ([email protected]) 125 The research of human Papillomavirus at the level of genital cancers by PCR and Amplicor test Lahouaria Belhabib ([email protected]) Contribution to the assessment of the level of contamination of the water quality of the Oran coast by 126 hydrocarbons and methodological developments for the extraction and analysis of hydrocarbons Nour El Houda Bousnane ([email protected]) 127 Study of seminal oxidative status and sperm DNA fragmentation in men with idiopathic infertility Mansouria Belmokhtar ([email protected]) 127 Role of Artemisia arborescens on Aluminium induced hepatic and renal dysfunctions in rats

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Fatima Zohra Hantour ([email protected]) 128 Current status of the use of local medicinal plants by breast cancer patients in Northwest Algeria Aicha Zellal ([email protected]) 128 Bioactive compounds of cyanobacteria synechocystissp isolated from west of Algeria Amani Amamra ([email protected]) 129 Contribution to the study of anti-microbieal activity of the aqueous extract of the white Artemisia Amira Kout ([email protected]) Research of Salmonella in chicken offal phenotypic characterisation and test of sensitivity to antibiotics 129 of isolated strains Mohammed ElAmin Said ([email protected]) Chemical characterization and antioxidant activity of the essential oil of Artemisia arborescens from the 130 region of Sidi Bel Abbes Dallel Arabet ([email protected]) Construction of a microbrial fuel cell using a strain of Exiguobacterium sp. isolated from Algerian Sahara 130

Housna Labed ([email protected]) 131 Healthcare waste management status in eastern algeria: a case study in the university hospital of batna Khedidja Tahar Djebbar ([email protected]) 132 Extraction and purification of Bordetella pertussis antigens responsible for whooping cough Meteoukki Wafaa ([email protected]) Contribution to the study of the involvement of rs 2070874 polymorphism of IL4 gene and rs rs3761548 132 polymorphism of Foxp3 gene in the development of keratoconus in a sample of the population of western Algeria Moubarek Mébarki ([email protected]) 133 Removal of Copper from Aqueous Solutions with Biomass from Forest by-product. Soumeya Chahed ([email protected]) 134 Biology and ecology of the Coot Fulica atra in the Djebla dam (Tizi-Ouzou, Algeria) Nabila Boukhari Benahmed Daidj ([email protected]) 134 The refining impact on the nutritional value and antioxidant potential of some Algerian local cereals Fatima Zohra Chabane ([email protected]) The effects of some bioactive compounds of star anise (illicumverum) and clove (Syrygiumaromaticum) 135 and their antioxidant potential Merad Yassine ([email protected]) 136 Prevalence of superficial mycosis in breast cancer patients: a cross-sectional study Sarah Khelfi ([email protected]) Molecular classification of breast cancer in the region of constantine: an epidemiological and 136 immunohistochemical study Aicha Sarah Lardjam-Hetraf ([email protected]) Effects of established blood pressure loci on blood pressure values and hypertension risk in an Algerian 137 population sample Malika Gherram ([email protected]) 137 The importance of data collection and statistical analysis of fishing and flotilla in western coast of Algeria Nassim Lahdiri ([email protected]) 138 Isolation of biosurfactant-producing bacteria from a hydrocarbon-contaminated area Dermeche Keltoum ([email protected]) 138 The teratogenic effects Of Thalidomide Enantiomers Soumia Ftouh ([email protected]) 139 Nhc gold complexes for biomedicals applications Meroua Horchi ([email protected]) 139 Parkinson's Disease: Epidemiological Characteristics of 63 Cases Lemya Boughrara ([email protected]) 140 Study of the biological activity of nitrogenous heterocyclic compounds Nil Soumia ([email protected]) Biodegradation of complex hydrocarbons in used engine oil by Gram negative bacteria isolated from 141 Oran harbour

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Saidi Yasmine ([email protected]) Genotypical identification and determination of technological properties of lactic acid bacteria isolated 141 from raw camel's milk Ghania Abid ([email protected]) Baseline risk factors that predict the development of Age-related macular degeneration in a sample of 142 the population of the east of Algeria Anfal Djouadi ([email protected]) Ameliorative impact of Omega 3 and Vitamin E co-treatment on fluoride induced oxidative stress and 143 renal cell carcinoma in rats Benfattoum Samia ([email protected]) 144 HLA-G as an inhibitor molecule of immune response in renal transplantation Amina Ammour ([email protected]) Study of genotypes and alleles distribution of CYP3A5 polymorphisms in the western Algerian 144 population: preliminary results. Ait Amar Samia ([email protected]) 145 Biology and diversity of potato aphids in Tizi Ouzou region Benchohra Hadria Amel ([email protected]) 145 Evaluation of the antibacterial activity of Artémisia campustris L Tamert Asma ([email protected]) Phytochemical screening and antimicrobial activity of extracts of Phlomiscrinita Cav., Mentha pulegium L. 146 and Origanum vulgare L. from the mount of Tessala (Western Algeria) Fatima Zohra Mahammi ([email protected]) 147 Genetic relationships and structuration of six African local chicken populations Nassima Brixi Gormat ([email protected]) 147 The relationship between sweet foods and non-alcoholic fatty liver disease in type 2 diabetics Sabrina Amara ([email protected]) Preliminary study on the probiotic effect of Lactobacillus plantarum NSC5C strain on neurobehavioral 148 disorders and oxidative stress induced by lead intoxication in Wistar rats Zouaouia Bettayeb ([email protected]) Phytochemical study and biological activity of a medicinal plant "Cotula cinerea" of the South-West of 148 Algeria Ouhaibi-Djellouli Hadjira ([email protected]) 149 Combined effect of established BMI loci on obesity-related traits in an Algerian population sample Khelifa Mahdjoubi Choukri ([email protected]) 150 Multi-Mycotoxin occurrence and risk characterization in foodstuffs from Algeria Attou Amina ([email protected]) Antioxydant Activity of the Essential Oil of Sature jacandidissima (Munby) Briq. Native Plant from West 151 Algeria Hania Rim Bahmed ([email protected]) Assessement of the knowledge of pharmacists community on oral anticancer therapy in the treatement 151 of breast cancer Kenza Zarour ([email protected]) Characterization and immunomodulation of exopolysaccharides-producing lactic acid bacteria strains of 152 technological interest Achou Rayene ([email protected]) 153 Alzheimer's disease: epidemiological characteristics Djouaher Thinhinane ([email protected]) Epidemiological survey of the seroprevalence of toxoplasmosis in pregnant women in the region of Tizi- 153 Ouzou Abismail Youcef ([email protected]) Beneficial Effect of Paronychia capitata L. aqueous Extract on Ethylene Glycol-Induced Urolithiasis in 154 Rats Haféda Ghorzi ([email protected]) 155 Effect of maternal exposure to a pyrethroid on the renal function of Wistar rat offspring Anfel Zerroug ([email protected]) Artificial neural network models based on QSAR for predicting and screening of novel AChE inhibitors as 156 anti- Alzheimer drugs

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Amir Manseur ([email protected]) 156 Study of the effects of lemon balm (Melissa officinalis) on long-term memory in Wistar male rats Lekmine Sabrina ([email protected]) 157 In vitro chemical and biological evaluation of a forage plant from arid zones Yasmine Zater ([email protected]) Antioxidant Property of Hydrolysates derived from the hydrolysis of Goat's Milk Caseins by Lactobacillus 157 Proteases Amira Imane Bahmed ([email protected]) 158 Evaluation of the antioxidant activity of the aqueous extract of Cucumis melo seeds Amira Imane Bahmed ([email protected]) 158 Evaluation of the antioxidant activity of the aqueous extract of Citrullus lanatus seeds Romyla Bourouba ([email protected]) Biochemical profile and risk factor’s assessment among women with breast cancer in Sétif, Algeria: case- 159 control study Aicha Hennia ([email protected]) Impact of plant protection products on some microorganisms in soil cultivated by potatoe (Solanum 159 tuberosum L. var Sylvana) Imene Benyettou ([email protected]) 160 Aluminium-induced acute neurotoxicity in rats: Treatment with omega-6/omega-3 ratio Bacha Mohamed El Amine ([email protected]) 160 Essential trace elements and medicinal tree: When is it safe to use? Fouad Menaceur ([email protected]) Neurotoxic effect of a mixture of two pesticides in rabbits and the protective role of a flavonoid: 161 Quercetin Guenaoui Mohamed ([email protected]) Contribution to the study of post mortem blood alcohol concentration from peripheral and central blood 162 in putrefied and non-putrefied cadavers Widad Sobhi ([email protected]) 163 QSAR Study and Molecular Modeling of POLO Like Kinase 1 inhibitors Hidaya Fatima Zohra Touil ([email protected]) 163 Inhibition of Candida albicans biofilm by synergistic action of exogenous terpenoids Widad Sobhi ([email protected]) 164 Neutral lipids for treatment of hyperglycaemia and dyslipidemia: First results on an animal model Djebara Nour EL-Houda Feriel ([email protected]) 164 Acute oral toxicity of the aqueous extract of Bryonia dioica roots Nawel Amraoui ([email protected]) 165 Inhibition of VEGF during angiogenesis Hadjer Boudjemaa ([email protected]) 166 Molecular characterization of multidrug resistant Proteus mirabilis isolates from Algerian patients Chahinez Hamedi ([email protected]) Complete chloroplast genome of the diatom Skeletonema pseudocostatum from the western 166 mediterranean coast of Algeria Baya Guermouche ([email protected]) 167 T-lymphocyte calcium signaling modulation by n-3 fatty acids in obese macrosomic rats Faiza Bendadeche ([email protected]) Genotyping by PCR-RFLP analysis of the 16S gene fragment of hydrocarbon-degrading bacteria isolated 167 from contaminated marine sediments at the port of Oran (Algeria) Abdellahi Ould Ahmed Salem ([email protected]) 168 Structural Targets Annotation Database – STAD & PR-Proteins selected targets Leila Saddikioui ([email protected]) 169 Relative Growth of Sepia officinalis Linnaeus, 1758 (Cephalopoda: Sepiida) from the Oran bay, Algeria Mahdi Souad ([email protected]) Evaluation of the antioxidant activity by DPPH and FRAP of the crude hydromethanolic extract prepared 169 by maceration of the aerial part of Salvia officinalis Youcef Benaissa Keddar ([email protected]) 170 Biological valorization for essential oils of TAFFS in the south-west Algerian

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Zoulikha Boucif Debab ([email protected]) 170 Cytogenetic and clinical study of Turner syndrome and klinefelter in the population of western Algeria Saidi Imene ([email protected]) 171 Antioxidant andanti-hemolytic effects ofphenolic compounds of Gleditsia triacanthos L. Pods Abbassia Benchaachoa ([email protected]) 171 Study of the antioxidant activity of extracts of scolymus hispanicus leaves Medjaher Halima Essaadia Souhila ([email protected]) 172 The antioxidant activity of the aerial part of mentha spicata in the region of AL BAYADH,ALGERIA Benmansour Nassima ([email protected]) Phytochemical analysis and evaluation of the antioxidant activity of the aqueous and methanol extracts 172 of Rhus pentaphylla Desf. Mohamed Ghermi ([email protected]) Genetic susceptibility to tuberculosis: Association of the polymorphism IL10 -1082 G/A to active 173 tuberculosis in a Western Algerian population. Rania Laouar ([email protected]) 173 Absence of Y-chromosome microdeletions in patients with Hypospadias with Chordee in Eastern Algeria Hayet Cherrad ([email protected]) 174 Antioxidant activity and phytochemical composition of two varieties of spinach Abdelkrim Berroukche ([email protected]) Experiments on Urtica dioica roots hydro-alcoholic extract activities against benign prostatic 175 hypertrophy-induced in mice by testosterone and a hyper-caloric diet. Phyto-chemical, Biological & Histological Studies.

14

Oran 05-07 December BIOSCIENCES 2019

PROGRAM

Saturday, December 7, 2019

08h00-09h30 Registration of participants OPENING SESSION - Speech of the Head of HSBSO, Pr. SAIDI. 09h30-10h00 - Speech of the head of the Thematic Research Agency in Health Sciences, Pr. N. AOUFENE. - Speech of the president of the Medical Oncology Society of Oran (SOMO), Pr. BOUSSAHBA. - Speech of the president of the organizing committee: Pr. BABA HAMED. PLENARY CONFERENCES PLENARY 1: Chair and Session Rapporteur: Pr. AOUFENE 10h00-10h30 Pr. Z.A. FOUATIH Apport du réseau national des registres du cancer au plan national cancer 2015-2019 10h30-11h00 Dr. J. ROBERT Oncogenèse et progression des cancers 11h00-11h30 Coffee break and Poster session I Chair: Pr. BOUDJEMA Pharmacogenomics /Oncogenomics Rapporteurs: Dr. BENAYAD- Dr. MAHDJOUR Novel approaches in cancer therapy Rapporteurs: Dr. BOULENOUAR-Dr. BENYETO PLENARY 2: Chair and Session Rapporteur: Pr. BOUSSAHBA 11h30-12h00 Pr. F. BEREKSI-REGUIG Tumeurs neuroendocrines 12h00-12h30 Dr. A. MEGAIZ, Pr. A. BOUSSAHBA Actualités sur l'immunothérapie dans le cancer bronchique 12h30-13h00 Pr. M. MEDJAMIA Rôle du pathologiste dans les testings moléculaires en cancérologie et expérience du service de Pathologie de l'hôpital militaire régional universitaire d’Oran 13h00-13h30 Pr. I BITAM Apport des nouvelles technologies en Oncologie 13h00-14h30 Lunch break

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PLENARY 3 : Chair and Session Rapporteur: Pr. BABA HAMED 14h30-15h00 Pr. A. AZIOUNE Les biotechnologies et l’écosystème national 15h00-15h30 Pr. M. ARIBI Tumor antigen cell lysate-pulsed classically activated M1 macrophages for vaccination against cancer 15h30-16h00 Dr. S. DJOULAH Bioengineering/IA et Oncologie, des métiers d’avenir pour les biologistes 16h00-16h30 DEBATE 16h30-17h00 Coffee break and Poster session II Chair: Pr. I BITAM Immunotechnology and cancer immunotherapy Rapporteurs: Dr. BOUGHRARA - Dr. MOGHTIT Cancer epigenetics / Nanotechnology in cancer treatment Rapporteurs: Dr. ILIAS - Dr. RAHLI

Sunday, December 8, 2019

PLENARY 4 : Chair and Session Rapporteur: Pr. SAIDI 09h00-09h30 Pr. D. CHARRON HLA & cancer à l'ère de la médecine de précision 09h30-10h00 Pr. T. MERGHOUB Develop means to overcome resistance to immune checkpoint blockade 10h00-10h30 Pr. A. BOUDJEMAA Pharmacogénétique et cancers : études algériennes 10h30-11h00 Coffee break and Poster session III Chair: Pr. S.DJOULAH Biosciences I Rapporteurs: Dr. MAHAMMI- Dr. CHOUBANE – Dr. KHELIL PLENARY 5 : Chair and Session Rapporteur: Pr. A.AZIOUNE 11h00-11h30 Dr. HIHI Ecosystèmes de recherche translationnelle : un modèle pour accélérer la R&D du laboratoire à l'usage médical 11h30-12h00 Dr. A. BOUAMRANI Brainprint: du concept clinique à la création d'une start-up 12h00-12h30 Pr. N. SADEGH Application de la nanotechnologie à la biologie et la médecine : nouvelles perspectives

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12h30-13h00 Pr. H BELHADJ TAHAR Conception et validations préclinique et clinique d'un nouvel agent anticancéreux issu de la nanotechnologie "Imdendrim" 13h00-14h30 Lunch break 14h30-16h30 4 oral communication workshops and a round table in parallel - Workshop 1: Oncogenomics, Pharmacogenomics, Cancer epigenetics and Nanotechnology in cancer treatment Rapporteur: Dr. N. Meroufel - Workshop 2: Novel approaches in cancer therapies I Rapporteur: Pr. N. Tabet Aoul - Workshop 3: Novel approaches in cancer therapies II Rapporteur: Dr. R. Abderrahmane - Workshop 4: Cancer diagnostic technologies, Immunotechnology and Cancer Immunotherapy Rapporteur: Dr. M. Abdi - Round table on the creation of a coordination of researchers in oncology of western Algeria 16h30-17h00 Coffee break and Poster session IV Chair: Pr. M. ARIBI Biosciences II Rapporteurs: Dr. MERZOUG – Dr. SADDIKIOUI – MAHMOUDI 17h00-17h30 Closing of the seminar

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Plenary Conferences

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Pr. Zoubir AHMED FOUATIH

Apport du Réseau National des Registres du Cancer au Plan National Cancer 2015 - 2019

Pr. Ahmed Fouatih is a teacher - researcher in Social Medicine - Epidemiology - Faculty of Medicine - Oran University 1 - Ahmed Benbella. He is also the Head of Department of Epidemiology and Preventive Medicine at CHU of Oran and the director of the research laboratory: "Surveillance of Infections related to care in Oran".

Dr. Jacques ROBERT

Oncogenèse et progression des cancers

Dr. Robert is a specialist in oncology. His research is devoted to identifying tools for predicting the activity of anticancer drugs in all areas of pharmacology: pharmacokinetics, metabolism and resistance, cellular and molecular pharmacology, and pharmacogenetics.

He presently works at the university of Bordeaux (France) and belongs to the unit 1218 of the INSERM (French National Institute of Health and Research) and continues his activities in the field of pharmacogenomics of anticancer.

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Dr. Abdelkader BOUSSAHBA

Actualités sur l'immunothérapie dans le cancer bronchique

Dr. Boussahba is the head of department of Oncology at the Hospital and University Center of Oran and the president of the “société d'oncologie médicale d’Oran S.O.M.O”

Pr. Faiza BEREKSI-REGUIG

Tumeurs neuroendocrines

Pr. Bereksi-Reguig is a apecialist of oncology and head of the Medical Oncology service at the Hospital-University Center of Sidi Bel Abbés (Algeria). She participated in several researches on cancer (breast, colon, lung). She is Vice President of the Medical Oncology Society of Oran (SOMO).

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Pr. Miloud MEDJAMIA

Rôle du pathologiste dans les testings moléculaires en cancérologie et expérience du service de Pathologie de l'hôpital militaire régional universitaire d’Oran

Pr. Medjamia is a doctor specialized in pathological anatomy. Presently he is the head of pathological anatomy department of the regional military hospital of Oran (Algeria). He is elected president of the Regional Pedagogic Committee of Specialty and draftsman of the National Pedagogical Committee of Specialty.

Pr. Idir BITAM

Apport des nouvelles technologies en Oncologie

Idir Bitam was trained by Philippe Parola and obtained his doctorate at the University of Aix-Marseille, he created JEAI MALBAVECT (2011-2014), a unique research team in entomology in Algeria. In 2014, this team was created as the main partner of the REMEDIER program (for REsearch for Mediterranean Infections Emerging or Reemerging) which was granted by A * MIDEX (No. ANR-11-IDEX-0001-02) funded by the investments for the future of the French State. In this context and alongside the creation of a doctoral program at the University of Sciences and Technology Houari Boumedien of Algiers, Idir Bitam was appointed visiting associate professor at the University of Aix-Marseille in 2015 and 2016.

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Pr. Ammar AZIOUNE

Les biotechnologies et l’écosystème national

Pr. Azioune is the head of the research center in biotechnology of Constantine (CRBT). Formerly researcher at the Université Victor Segalen Bordeaux (France), LISE Laboratory University of Namur (Belgium) and Centre of Molecular and Macromolecular Studies in Łódź (Poland).

Pr. Abdallah BOUDJEMAA

Pharmacogénétique et cancers: études algériennes

Abdallah Boudjema currently works at the génétique moléculaire appliquée, Université des Sciences et de la Technologie d'Oran Mohamed Boudiaf. Abdallah does research in Biostatistics, Molecular Biology and Bioinformatics. Their current project is 'Immunogenetic study of ankylosing spondylitis'. also, I am interested in the pharmacogenetics of molecules used in autoimmune diseases and in cancer.

22 Oran 05-07 December

Dr. Sami DJOULAH

Bioengineering/IA et Oncologie, des métiers d’avenir pour les biologistes

Dr. Djoulah is an immunogenetician and creator of the company "Wiratech Europe" specialized in NGS (Next Generation Sequencing) and immunogenetic analysis for a precision medicine and a personalized diagnosis. He is currently Chairman at the France Genomics 2025 Project.

Pr. Dominique Charron

HLA & cancer à l'ère de la médecine de précision

Pr. Charron is a specialist in immunology. He is actually a professor Emeritus of medecine at the University of Paris Diderot (France) and professor advisory at the Shanghai JiaoTong University (China). He is a medical advisor at APHP General Directorate and co-founded the transplantation rejection laboratory of Excellence TRANSPLANTEX.

23 Oran 05-07 December

Pr. Taha MERGHOUB

Develop means to overcome resistance to immune checkpoint blockade

Pr. Merghoub is a specialist of human onclology and his research projects focus on investigating the means for developing immune-based therapies to treat cancer. He leads the Ludwig Collaborative Laboratory and the Swim Across America Laboratory at Memorial Sloan Kettering in New- York (USA).

Pr. Mourad ARIBI

Tumor antigen cell lysate-pulsed classically activated M1 macrophages for vaccination against cancer

Pr. Mourad Aribi is a professor of Immunology at the University of Tlemcen (Algeria). He is also the founder and director of the Laboratory of Applied Molecular Biology and Immunology. His current research focuses on the modulation of cell-mediated and inflammatory immune responses in autoimmune diseases, cancer diseases, and infectious diseases.

24 Oran 05-07 December

Dr. Abdelmadjid HIHI

Ecosystèmes de recherche translationnelle : un modèle pour accélérer la R&D du laboratoire à l'usage médical

Dr. Hihi is a specialist in molecular genetics of nuclear hormone receptors. He conducted researches on genetic mechanisms of aging. He participated in creating companies such as: Chronogen inc. (Canada) and Génome Québec (Canada). He is currently Head of programs at CEA-LETI, Clinatec, Grenoble (France). Dr. Hihi is a lecturer at universities and management schools in Algeria, Japan and France.

Dr. Ali BOUAMRANI

Brainprint: du concept clinique à la création d'une start-up

Dr. Bouamrani is a researcher at the Office for Atomic Energy and Alternative Energies (CEA Genoble_ France). He is the Co-founder of MEDIMPRINT (Grenoble_ France) a company that develops innovative medical devices allowing the analysis of previously inaccessible brain regions.

25 Oran 05-07 December

Pr. Noureddine SADEG

Application de la nanotechnologie à la biologie et la médecine : nouvelles perspectives

Pr. Sadeg is a specialist in Emergency Toxicology, Addiction Medicine and Clinical Pharmacology. He currently works at the pharmacologie toxicologie, as CEO of International Medical Institute of Toxicology and Pharmacology I-MITOX. He is also the president of the company NANO GUN TECHNOLOGY : NGT (France).

Pr. Hafid BELHADJ TAHAR

Conception et validations préclinique et clinique d'un nouvel agent anticancéreux issu de la nanotechnologie "Imdendrim"

Pr. Belhadj Tahar is the president of the French Association of Medical Research Advancement (AFPREMED) since 2007 and current president of COLCOM (laboratory of Nanotechnology for biological applications). He had graduated from Tlemcen University, School of Medicine in 1980. After obtaining degree of Doctors of Medicine in 1987, he has been specialized in the oncology and radiotherapy in Toulouse (France). He got the oncology specialization diploma in 1991 and PhD of radiochemistry in 1996 at Grenoble University, and engaged as researcher in radiochemistry and radiopharmaceutics field in national center of scientific research (CNRS of Toulouse).

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Oral

Communications

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Oncogenomics and Pharmacogenomics

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O001 in disagreement with the result found for the Mozabite ethnic group (MAFs =18%). However, Association of CHRNA5 rs16969968 close to frequencies found in the European and Polymorphism and Predisposition to Lung Middle Eastern population, (MAFs=37%–43%). Cancer in Algeria The rs16969968 polymorphism do not show any significant association with NSCLC risk, but 1,2* 3 Mimouni Asma , Rouleau Etienne , Saulnier stratified analysis showed that persons carrying 3 4 5 Patrick , Filali Taha , Beddar Leila , Lakehal the AA genotype are significantly associated with Abdelhak6, Marouani Amina7, Haddad Bahria8, adenocarcinoma risk (pAdj=0.03, ORAdj = 2.55). Hireche Ahmed1,2, Boudokhane Ibtissem1,2, Boudersa Falvella et al. and reported that CHRNA5 mRNA Asma9, Aissaoui Mahmoud10, Ramtani Hacene10, levels are up-regulated 30-fold in ADK compared Abdelali Med Lamine11, Bouhedjar Khalid12, with normal lung tissue in individuals carrying Abdellouche Djamel13,Boudjehih Messaouda14, Abadi AA genotype compared with those having GG Noureddine2, Satta Dalila1,2 genotype which accords with our results. Nevertheless, Jaworoska et al. showed that this 1. Laboratoire de Biologie Moléculaire et Cellulaire Univ. locus is implicated in all histopathologic subtypes Frères Mentouri Constantine 1, Algérie. of LC. In our study, we found a significant 2. Laboratoire de Biologie et Génétique Moléculaire, Univ. Constantine 3, Algérie. association between the CHRNA5 rs16969968 3. AMMICa/US 23 INSERM / UMS 3655 CNRS, Paris, France. polymorphism and the increase of lung 4. Service d’oncologie CHU Constantine, Faculté de médecine adenocarcinoma risk. Additional studies on large Univ. Constantine 3. Algérie. population are needed to confirm our results. 5. Service d’anatomie pathologique, CHU Constantine. Faculté de médecine Univ. Constantine 3, Algérie. 6. Registre de cancer Constantine et Service d’épidémiologie et Keywords: CHRNA5; Non-small cell lung cancer; médecine préventive, CHU Constantine, Algérie. Predisposition; rs16969968. 7. Service de pneumologie, CHU Sétif. Algérie. 8. Service de pneumologie, CHU Batna, Algérie. 9. Service d’oncologie, CHU Annaba.Algérie. 10. Clinique Alfarabi, Annaba, Algérie. O002 11. Physics Depart. Univ. Frères Mentouri Constantine 1. The frequency of EGFR mutations in lung Algérie. 12. Centre de Recherche en Biotechnologie, Constantine. carcinoma in the west Algerian population Algérie. 13. Service D’anatomie pathologique, CHU Sétif, Algérie. Fatima Zohra Moghtit1,2,3*,Wefa Boughrara1,2,4,Ilies 14. Registre de cancer Batna et Service d’épidémiologie et Koriche2,Imene Ouhba2, Amina Mama Boubekeur1, médecine préventive, CHU Batna, Algérie. 1 1 [email protected] Djabaria Naima Meroufel , Abdallah Boudjema , Faouzia Zemani1, Meriem Samia Aberkane 1,2,5. Abstract Lung cancer remains the most common cancer in 1Laboratoire de Ge´ne´tique Mole´culaire et Cellulaire, Université des Sciences et de la Technologie d’Oran– the World. As for Algeria, it is the first cause of Mohamed BOUDIAF-USTOMB, BP 1505, El M’naouer, 31000 mortality from cancer for men and the seventh Oran, Algérie. for women. The genetic polymorphism 2Laboratoire de cytogénétique et biologie moléculaire,EHU, rs16969968 in CHRNA5 was shown to be BP4166 Ibn Rochd, 31000 Oran, Algérie. 3 strongly associated with the risk of lung cancer. Centre universitaire d’Ain Témouchent,BELHADJ Bouchaib. BP284,46000,Ain Témouchent, Algérie The aim of our study is to investigate how this 4Ecole supérieure en sciences bioloiques d’Oran , BP 1042 polymorphism can modify the risk of non-small SAIM Mohamed 31003 Oran, Algeria cell lung cancer (NSCLC) in the Eastern Algerian 5Département de Pharmacie, Faculté de Médecine, BP 1524, El population. To date, no study has considered this M’naouer, 31000 Oran, Algérie association in the Algerian population. We have [email protected] performed a case control study of 144 lung Abstract cancer patients and 211 healthy. Genotyping was Mutation analysis of epidermal growth factor done using TaqMan probes and the data were receptor (EGFR) gene in non-small-cell lung analyzed using multivariate logistic regression cancer (NSCLC) has become important for adjusted for covariates. The minor allele predicting response to treatment with tyrosine frequencies (MAFs) of CHRNA5 rs16969968 kinase inhibitors. however, their prevalence polymorphism in controls were A: 31%, which is varies with ethnicity. Little is know concerning 29

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their prevalence in Arab populations. The of molecular biology and genomics a new intention of this population study is to determine discipline has just been born, it is the frequency of EGFR mutation in NSCLC in a Pharmacogenomics. It aims to study the role of group of West Algerian patients. Tumr DNA was the genome in the response to drugs. It combines extracted from one hundred twenty FFPE tissue pharmacology and genomics by analyzing the samples and analyzed for EGFR mutationnal impact of the patient's genetic heritage on its status by real time PCR. A total of 15 EGFR response to drugs thus allowing the correlation mutations were found in this series of 120 between gene expressions, SNPs, patients (12,5%). The most frequent EGFR epigenetics...etc. with the pharmacokinetics mutation was detected in exon 19 (80%) (adsorption, distribution, metabolism and followed by exon 21 (13%) and exon 18 (7%), elimination of the drug) and the more frequently in men (67%) than in women. pharmacodynamics of the drug. This study This prelimenary epidemiological study is the focuses on the solutions and benefits of first report on the prevalence of EGFR mutations Pharmacogenomics in oncology and how to in Algerian patients. It reveals a prevalence of elucidate the genetic bases for interindividual 12,5%. The most frequent EGFR mutation was differences and to use such genetic information exon-19 deletion. Further studies, in larger to predict the safety, toxicity, and/or efficacy of numbers of patients are needed to confirm these drugs. findings Keywords: Cancer; Drugs; Genomics; Keywords: Lung cancer; EGFR mutations; Pharmacogenomics. frequency; West Algerian population.

O004 O003 New cancer therapy strategy by targeting Spectacular advances in genomics in favor of rescue molecules and dormancy the implementation of Pharmacogenomics Djeda Belharazem 1*, Zhang Xiaonan1, Berthold Tifrit Abdelkrim*1, Drici Amine El Mokhtar 2, Schalke2, Thomas Gräter3, Thomas Kyris4 and Aberkane Meriem 3. Alexander Marx1.

1University of Ahmed Ben Bella, Oran1. Faculty of Medecine 1 Institute of Pathology and Medical Research Center (ZMF), department of Pharmacy University od Medicine, Mannheim, Germany 2University of Djillali Liabes Sidi Bel Abbes, Faculty of sciences, 2 Department of Neurology, University of Regensburg, department of Biology Regensburg, Germany 3University of Ahmed Ben Bella, Oran1. Faculty of Medecine 3 Department of Thoracic Surgery, Clinic Löwenstein, department of Pharmacy Löwenstein, Germany [email protected] 4 Department of Thoracic Surgery, Clinic Schillerhöhe, Robert- Bosch-Hospital, Gerlingen, Germany [email protected] Abstract Nowadays, cancer ranks the second place in the Abstract mortality podium with an estimated 9.6 million deaths in 2018 (WHO). Despite the innovative The mono-therapy approach is still a very advances in the implementation of oncology common treatment modality for many different treatments, there are significant cases of forms of cancer[1]. Combination therapy, a therapeutic failure. Several researches on the treatment modality that combines two or more inefficiency of anti-cancer cures point to the therapeutic agents, is a cornerstone of cancer genomic particularity of each patient that therapy. This approach potentially reduces drug modulates the response to the drug as well as the resistance to sunitinib in thymic tumors [2], access to anti-cancer treatments which is which are thymic epithelial tumors with poorly extremely expensive and makes the treatment understood pathogenesis and no curative options inaccessible especially in low- and middle- beyond surgery[3]. The combination therapy income countries. With the spectacular advances using multitargeted kinase inhibitor Sunitinib and BCL2 inhibitor obatoclax or MIK665 30

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inhibitor of MCL1 induce antiproliferative more thymic spheres formation after sunitinib activity and apoptosis and blocke spheres treatment. Synergy therapy seems to be forming in ex vivo primary Epithelial thymic promising for thymic tumor therapy tumor cells. Primary tumor Epithelial cells development due to targeting rescue mechanism (pTECs) were isolated from fresh tumor tissues developed during the phenomenon called and treated using several drugs: Sunitinib as therapy induced senescence, this could be a hint tyrosine kinase inhibitor (RTK), obatoclax as to the presence of distinct cell populations in the BCL2 inhibitor and MIK665 as MCL1 inhibitor in tumor, thymic cancer research has to face the single or combined therapy. Cell proliferation major challenge of targeting the intra-tumoral as and cell death were analyzed by MTT assay and well as inter-tumoral heterogeneity. Thus, FACS. pTECs were cultured in 3D spheres targeting molecular drivers operating in the systems before and after any drug treatment. All different cell population including cancer stem the thymoma pTECs showed resistance to cells, in combination with standard treatments, sunitinib, Obatolax and MIKK665 when used as may improve cancer patients’ outcomes, yielding single therapy. The cells showed either BCL2 or long-lasting responses. MCL1 overexpression in different groups of pTECs when treated with sunitinib as rescue Keywords: Synergy therapy, therapy rescue mechanism induced after the first therapy. molecules, RTK inhibitor and antiapoptotic Synergy therapy combining sunitinib with inhibitor Obatoclax or sunitinib with MIK665 in low dosis showed drug sensitivity and open new therapy strategy targeting the two rescue molecules BCL2 and MCL1 in thymic malignancies. pTECs showed

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Cancer Epigenetics and Nanotechnology in cancer treatment

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O005 O006

Development of Transplantable Tumoral Genetic and epigenetic profile of gastric Graft in Immune-compromised Wistar Rats carcinoma infected with Helicobacter pylori in an Algerian population Boubekeur Y.1, Boutouha L.1, Aouragh K.1, Nacer- Amine El-Mokhtar Drici 1*; Abdelkarim Tifrit 2; Khodja A.1, Hamai H.1, Abdelhafid K.1, Allali S.1, Mustapha Diaf 1; Djamila Titsaoui 3; Abdenaceur Tou 4; Adjerad A.2, Abdoun K.1, Mameri S.3, Chader H.4 and Laradj Arbaoui 5; Soraya Moulessehoul1. Djefal-Kerrar A.1

1Medical Applications Department, Nuclear Applications Department of Biology, Faculty of Sciences of Nature and Life, Division, Nuclear Research Center of Algiers, Algiers, Algeria. Djillali Liabes University of Sidi Bel Abbes, Sidi Bel Abbes, 2Specialised Technical Division, Nuclear Research Center of Algeria. Algiers, Algiers, Algeria. Department of Pharmacy, Faculty of Medical Sciences, Ahmed 3Department of Pathology, University-Hospital Center, Ben Bella University Oran 1. Oran, Algeria. Algeria. Gastroenterology Service, Abdelkader Hassani Teaching 4National Pharmaceutical Control Laboratory, Algiers, Hospital of Sidi Bel Abbes. Sidi Bel Abbes, Algeria. Algeria. Pathology Service, Abdelkader Hassani Teaching Hospital of [email protected]. Sidi Bel Abbes, Sidi Bel Abbes, Algeria. Medical Oncology Service, Misserghine Oran Anti-Cancer Abstract Center, Oran, Algeria. [email protected] Cancer modeling in laboratory-animals, particularly rats, has several limitations. These Abstract are related to the inbreeding nature of those Gastric carcinoma is a biologically heterogeneous animals, which does not adequately assess the disease involving many genetic, epigenetic and contribution of host genetic heterogeneity during environmental alterations. Pro-inflammatory tumor growth. The present work aimed to cytokines SNPs appear to be factors in develop a locally available animal model for maintaining and promoting Helicobacter pylori cancer research. The investigation involves a (H. pylori) infection. This etiologic factor plays a series of transplantable cancerous cells, using role in the transcriptional inactivation of tumor subcutaneous Hep-2 cells inoculation (xenograft) suppressor genes by DNA hypermethylation. A and murine tumor cells derived from murine case-control study was conducted to elucidate solid tumor (allograft) in cyclosporine immuno- the effect of these polymorphisms and DNA deficient female Wistar rats. Analytical follow-up hypermethylation on carcinogenesis of the focused on animal growth, tumor development, gastric mucosa in association with HP infection metastasis, survival, histopathology and in an Algerian population. The analysis was immuno-histopathology of tumors. It has been carried out on 79 HP-infected patients with successfully demonstrated that cyclosporine chronic gastritis and gastric carcinoma, and 32 mediated immune-compromised female wistar subjects were recruited as case-control. The bi- rats support the growth of human epidermoid allelic SNPs of IL-1β (-31 and -511), TNF-α (-308 carcinoma cells (HEp2) resulting in tumor and -1031) and the penta-allelic polymorphism masses. Transplantation of these allografts has of IL-1RN were genotyped. The identification of caused epithelial malignancies. Histopathological the hypermethylation of the promoter of the and immune-histochemical analysis of xenograft COX-2, CDKN2Ap16, APC, BRCA1, CDH1 and and allograft revealed a clear presence of hMLH1 genes was carried out by Methylation- malignant epithelial tumor. The present animal specific PCR (MSP) and the subtyping of H. pylori model has several advantages, timeliness of was carried out. The IL-1β-31 * C and IL-1RN * 2 tumor appearance, easily and simply detectable genotypes were associated with an increased risk by palpation due to its superficial location at the of developing gastric carcinoma. However, IL-1β- subcutaneous injection site. 511 * T has been associated with an increased risk of developing chronic atrophic gastritis. Keywords: Animal model, immune- Promoters of the hypermethylated COX-2 and compromising agents, tumor induction, hMLH1 genes were associated with the xenograft. development of gastric carcinoma, infected with H. pylori vacA s1/m1, confirming the

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involvement of this event in the inactivation of labeling in the dosimetric study. In our study, we these genes, as observed by other authors. This analyzed radionuclide chromosome study demonstrates that the presence of certain preparations, obtained previously by irradiated pro-inflammatory cytokine polymorphisms in samples at 2Gy, 5Gy and 8Gy doses of γ-gamma the host and the hypermethylation of DNA in a irradiation in the laboratory of the Department of mechanism of inactivation of tumor suppressor Biology UAB, Spain. The metaphase plates were genes are crucial in gastric mucosal prepared according to two staining protocols. carcinogenesis which depends on the genotype of The first is based on a Giemsa uniform stain and H. pylori. A better understanding of DNA the second is based on probes using the FISH hypermethylation will open up new diagnostic technique. The staining of telomers and perspectives and highlight therapeutic targets in centromeres (tc) not only makes it easier to the treatment of gastric cancer in precision detect dicentrics and rings but also renders the medical part in our population. scoring of radio-induced more reliable. The analysis focused on more than 2000 metaphases Keywords: DNA hypermethylation; Gastric observed on slides prepared according to the Cancer; Helicobacter pylori; SNP. staining protocols mentioned beforehand. A statistical analysis was undertaken taking into account the frequency, the Poisson's law, the standard error and Papworth's U calculation. O007 Thus making it possible to confirm the New biological dosimetry tool for detecting homogeneity of the irradiations on our samples. We then used FISH tagging to explore the radio-induced aberrations telomeres and centromeres and compared the Ahlem-Nora Messal1,2,3*,Lotfi Louhibi13,AbdAllah two techniques by evaluating the benefits in Boujemaa1,2,3 ,Nadhira Saidi-Mehtar1,2, Zoulikha dosimetric analysis. Our results show that the Boucif5 , FaouziaZemani1,2,3, Joan Francesc frequencies of dicentric chromosomes labeled in Barquinero4. FISH are greater than those obtained in standard staining. This study allows long-term monitoring 1 Universty of Science and technology Mohamed of populations exposed to radiation, which Boudhiaf Oran, Algeria. 2 Laboratory of molecular and cellular biology. contributes to the definition of the risk 3 Thematic Agency for Research in Health Sciences associated with the use of chemical agents and ATRSS. ionizing radiation, particularly at low doses. In 4 Unitat of AntropologiaBiológica. Department of addition, the evaluation of a dose of irradiation Biologia Animal, Biologia Vegetal iEcologia. by the FISH technique is faster and more reliable. UniversitatAutònoma de Barcelona, E-08193, Bellaterra, Spain. Which would lead to the care of the irradiated 5 Cytogenetics Laboratory, Ophthalmology persons in the case of an accident of irradiation Specialized Hospital Establishment in the shortest possible time such as the HammouBoutliliss, Oran. irradiations resulting from the radiotherapy *[email protected] which can be at the origin of the induction of

certain cancers, as well at the patients than at the health personnel. Abstract Biological dosimetry is a discipline that Keywords: Biodosimetry; ionizing radiation; determines the dose of ionizing radiation FISH ; cancers. received by an individual whether natural, accidental or occupational, from biological parameters. It is based on the analysis of radioinduced aberrations, mainly dicentric chromosomes, which are considered as true bioindicators of acute and recent irradiation, because they are very rare in the natural state. Our goal was to evaluate the efficacy and demonstrate the advantage of the FISH technique using probes allowing telomere and centromeric

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O008 O009 New design for synthesis of anti timor nano Metabolic Treatment against Cancer heterocyclic molecules called frog, fox and bat Methylglyoxal, a new target in the catalyzed by maghnite (Algerian MMT) hepatocarcinoma treatment: An alternative approach for coumarin drug carriers Abdelkader Rahmouni and Mohammed Belbachir Nadia Taïbi 1,2*, Nadjia Bekari8 , Ghazi Al-Jabal4, 1 Laboratory of Polymer Chemistry, Department of Chemistry, Oualid Talhi1 , Yasmine Benali7 , Qosay Al-Balas4 , Faculty of Science, University of Oran 1 Ahmed Benbella. BP N Rachid Ameraoui1 , Mohamed Hadjaj1 , Amina Taibi6 , ° 1524 El M’nouar, 31000 Oran, Algeria Mouna Boutaiba1 , Mohamed Abou-Mustapha1 , [email protected] Ahmed Abu-Alhaija5 , Ammar Almaaytah5, Farida Abstract Khammar2 , Fayçal Dergal1 , Redha Hassaine1 , Leila Boukenna1 , Khaldoun Bachari1 , Artur Manuel Soares The use of phenolic compounds as natural Silva3. antioxidants in food is in progress in relation to their role in prevention of some cancers and 1 Centre de Recherche Scientifique et Technique en Analyses cardiovascular diseases. The aim of this work Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, describes new and efficient strategy for synthesis Algérie. 2 Université des Sciences et de la Technologie Houari of new anti timoral nano heterocyclic molecules Boumediene (USTHB), Faculté des Sciences Biologiques (FSB), called frog, fox and bat using Maghnite (Algerian Laboratoire de Recherche sur les Zones Arides, (LRZA), BP 32 MMT) as friendly catalyst. The Maghnite-Na + El Alia 16111, Bab Ezzouar 16111, Algérie (MMT-Na +) used in this work came from a 3 Department of Chemistry, University of Aveiro, 3810-193 quarry located in Maghnia (North West of Aveiro, Portugal 4 Department of Medicinal Chemistry and Pharmacognosy, Algeria) and was supplied by the company Faculty of Pharmacy, Jordan University of Science and "ENOF" (Algerian manufacture specialized in the Technology, Irbid, Jordan production of non ferrous and useful 5 Department of Pharmaceutical Technology, Faculty of substances). Our method of synthesis must Pharmacy, Jordan University of Science and Technology, Irbid, Jordan include points of view on health and the 6 Laboratoire de Parasitologie et Mycologie, Laboratoire de environment that is why we used a catalytic Recherche « Santé et production Animale, École Nationale support, heterogeneous and ecological it is the Supérieure Vétérinaire, B.P. 228, Oued Smar- Alger, Algérie. maghnite. chemical results have been 7 Laboratoire d'Anatomie et Cytologie Pathologiques Vétérinaires. Département de Microbiologie et Pathologie summarized by above figures above and Vétérinaire. Institut Pasteur d'Algérie. 1 rue du Docteur biological tests reported positive test such LAVERAN. El Hamma. Alger. solubility and toxicity and good reaction with 8 USTHB, Faculty of Biological Sciences, Laboratory of cellular DNA support such as stopping cell divisions and and Molecular Biology,16111, BP32, El Alia, Bab Ezzouar, rearrangement of DNA genes of a cancer cell .The Algiers, Algeria structures of these compounds were confirmed [email protected] by FT-IR and NMR spectroscopy. A synthesized materials having good physical chemical Abstract properties such as water solubility at room Methylglyoxal is a cytotoxic dicarbonyl temperature and good biological properties such compound, naturally present in cells and as the low degree of toxicity which is tested on a pathologically increased in oxidative stress. In bacterium and a rat. fact, methylglyoxal is considered as one of the powerful precursors of the glycolytic pathway; it Keywords: Maghnite, heterocyclic, design, is formed by a non-enzymatic fragmentation of nanomaterials, orcenol, anti cancer. the triose phosphates and played a central role in the detoxification process. The abnormal

accumulation of it leads to increased protein and

DNA modification and contributes to cell and

tissue dysfunction in ageing and disease. The aim

of our study is to use the anticancer strategy of

methylglyoxal on chemically induced

hepatocarcinoma on Wistar rats to evaluate the

inhibitory effect of OT-55 on the glyoxalase

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system. According to the NIH, hepatocarcinoma as depicted by pyknotic peripheral nucleus, a is the most common primary malignant liver translucent cytoplasm and the appearance of a cancer and is the third cause of cancer mortality pseudoglandular formation. As expected, in the world, its multifactorial pathogenicity proteins show a non-statistical correlation make it difficult to diagnosis. Liposomes were between treated and untreated rats. However, prepared by the dehydration-rehydration we note a correspondence between these two process and characterized, their content on OT- parameters since albumin (594.96 vs 621.59 55 was quantified by HPLC in the same way as µmol.l) and protein (7.73 vs 8.06 g.dl-1) express in the MGO. Following carcinogenesis procedure, the same way. Preliminary results show twenty females were treated with 200 µl of simultaneously in the control and treated groups liposomal suspension. After scarifying, tissues that OT-55 would probably act on protein were removed fixed in formalin and frozen. The synthesis in addition to blocking GLO1 confirmed hepatic enzymes and glucose were quantified on in vitro. Same phenomenon was reported in both kits. Histology was performed at IPA. For induced and induced-treated groups. We rightly statistical analysis, one way-ANOVA test was believe that OT-55 has a dual role - to kill cancer used. cells and save healthy cells. Methylglyoxal has SEM and DLS evaluation confirms the presence of been reported to induce glycation and droplets sized between 75 and 134 nm. HPLC aggregation of serum proteins in the rat. We also analysis confirmed that 50% of OT-55 was demonstrated a decline of all liver enzymes in trapped in the lipid bilayer, as described by. treated. Beyond its cytotoxic role, OT-55 can be Wistar rats treated with a classical carcinogen also proposed as an angiogenic factor. In (DEN) showed the first microscopic signs of perspective, it would be interesting to list MGO hepatocarcinoma. Pre-cancerous lesions on non- as routine laboratory analysis and considered it cirrhotic liver have developed. In OT-55 treated as a biomarker. rats, the enzymatic activity of GLO1 was inhibited Keywords: Coumarin-carriers; Hepatocarcinoma; according to plasmatic methylquinoxaline Methylglyoxal; Wistar albino’s rat concentration (2.55 vs 1.11pmol.µl.-1) compared to untreated group. In fact OT-55 reduce indirectly glucose consumption (5.26 vs 5.02 mmol.l-1), lactate deshydrogenase production (229.51 vs 234.29 UI/L) and impaired ATP supply necessary for cancer cells proliferation. On histological section, MGO generates apoptose

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Novel approaches In cancer therapies

37

Oran 05-07 December

O010 O011 Herbal medicinal plants using by cancer Study of antioxidant effect from Algerian patients at Oncology Department of CHU medicinal plants Blida, Algeria Nadia. Kambouche1*, Bakhta Ramli1, El Houaria Feli djMenel1, Babahamed Mohamrd bey1,ABI-AYAD Benzineb1, Mohamed Talbi1, Salima Bellahoue1,

Sidi-Mohammed El-Amine2 1 Université Oran 1. Faculté des Sciences Exactes et Appliqués. Laboratoire de Synthèse Organique et Appliqué. Département 1Laboratory of Aquaculture and Bioremediation (AquaBior), de Chimie Department of Biotechnology, University of Oran 1, Oran- 1524 Oran Es Senia El M'naouer ALGERIA. Higher School of Biological Sciences of Oran [email protected] (HSBSO), Oran- ALGERIA ² Laboratory of Aquaculture and Bioremediation (AquaBior), Department of Biotechnology, University of Oran 1, Oran- ALGERIA. Abstract

[email protected] In this study involving the search of new natural therapeutic value of three plant species, namely Inula viscosa, Artemesia campestris and Anabasis Abstract articulata, selected on the basis of their In Algeria, herbal medicinal plants are using by a widespread use in local traditional medicine lot of patient because medicinal plants are very have subject to phytochemical screening to rich in source of bioactive pounds. Our study highlight the different phytoconstituants present aims breast cancer patients using medicinal in these plants. The essence of the plant I. viscosa plants in hospital (CHU Blida) during extracted by hydrodistillation using Clevenger chemotherapy for 7 years (2012/2018). In our apparatus is obtained in quantitative yield and study, we have done investigations on using comparable to previous work. The application of medicinal plantsby cancer patients at oncology gas chromatography coupled with mass department of CHU Blida,1000 breast cancer spectrometry (GC / MS) has allowed the patients was questioned, 85% of them report identification of 21 compounds. Evaluation of have used medicinal plants without informing antioxidant activity of aqueous extracts of three them doctors. As a result we can say that this plants was addressed in two ways: practice can be very dangerous in chemotherapy • The test of DPPH. period because of interference between natural • Radical-Scavenging Activity molecules and chemical one. The breast cancer Our results demonstrate the antioxidant activity patients can have a lot of problems like liver in vitro by inhibiting the oxidation power of activity, digestive problems and can cause death DPPH. The IC50, respectively, for plant Inula of patients. viscosa, Artemesia campestris are 2.24 mg / ml and 2.8 mg / ml. In light of these results we can Keywords: medicinal plant; breast cancer; highlight the protective power of new natural Chemotherapy. antioxidants of these plants against the onset and progression of certain diseases related to oxidative stress.

Keywords: phytochemical study, essential oil, GC / MS, flavonoids, antioxidant

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O012 had little inhibition to normal keratinocyte (No cytotoxicity inhibitory activity on normal cells by Cytotoxicity, Anticancer Dose-time manner live dead method has been reported with IC50, and Apoptotic Activity in Human Breast after 72 hrs); This concertation was used as Cancer Cells by Natural products Derived induced apoptosis. In addition, the percentage of from Medicinal Herbs Extracts late apoptotic cells for irreversible pathway by caspase-3/7 is 40.5 %, 65 % and 78% after 24h, Dehimat Abdelouahab1,2*, Azizi Ines1, Boukhaddaoui 48 and 72 h, respectively. While, the earlier Hassan3, Véronique Barragan-Montero4, Khettal apoptotic cells by Annexin V-FTIC 57 %, 69% and Bachra2. 91% for NPs. For the necrotic cells were determined by IP, 23%, 29% and 37% of cells 1Département des Sciences de la Nature et de la Vie, Faculté were IP+. Despite, the paclitaxel used as a des Sciences Exactes et des Sciences de la Nature et de la Vie, Université de Biskra, Biskra 07000, Algérie . positive standard. In a study carried out by 2Laboratoire de Biotechnologies Végétales et Ethnobotanique, Montero team were used the paclitaxel (Taxol), Faculté des Sciences de la Nature et de la Vie, 3Université de derived from Taxus brevifolia (from the family Bejaia, Bejaia 06000, Algérie. Taxaceae) and currently used in the treatment of 4 INM, Université de Montpellier, Montpellier, France. breast cancer. In conclusion, our findings 5LBN, Université de Montpellier, Montpellier, France. [email protected] underscore that NPs suppressed, in a dose- and time-dependent manner, the proliferation and Abstract colony formation in the breast cancer cell line. Breast cancer is one of the most prevalent The cell death was caused by induction of malignant diseases in women nowadays and its apoptosis through externalization of PS and occurrence is rapidly increasing due to the caspase 3/7 activation which are cancer-specific stresses of modern life. Fortunately, targets for cancer therapy. Natural product from biotechnology products derived from medicinal leaves extract could be expected to have a currently account for around half the profits therapeutic effect and maybe will be used as a generated by the “new” biotechnology industries. drug of cancer treatments. Further in vitro and In order to increase the effectiveness of cancer in vivo experiments are warranted to resolve treatment, the interest has been recently focused precise mechanisms responsible for its on those drugs that have been used in traditional anticancer effects on gene expression. medicine to manger breast cancer disease. Breast human adenocarcinoma cell line (MCF-7) were Keywords: MCF-7 cells; Cytotoxicity; Apoptosis; used to evaluate the cytotoxicity of Natural Natural product. products derives from leaves extract of an endemic plant (NPs) using MTT assay in dose and dose-time manners (for 4 to 96 hours of exposure). Apoptosis was further confirmed and quantified by caspase-3/7 activation and by Annexin-V FITC binding of externalization of phosphatidylserine (PS) using the immunofluorescence staining. IP was used to detect the necrosis. The normal cell used to evaluate the cytotoxicity of NPs using Live/Dead ThermoFisher assay. Untreated and treated cells were evaluated for apoptosis by fluorescence microscopy. Zeiss (Zen) and Imaris© Software were used to treated images and counting labeled cells. All NPs decreased cell growth of MCF-7 cell line in a concentration-dependent manner. A less time exposure (just 4 hours) the NPs were capable to inhibit the MCF-7 proliferation with low concertation. The NPs exhibited a marker anti-proliferative effects on cancer cells in dose and time-dose manner, and

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O013 apoptotic mechanism of action in HCT-116 cells. The selectivity of action of these compounds Real-time monitoring by electrical impedance against certain cancer cell lines are dimensions spectroscopy of cell viability during treatment that we intend to explore in future. with Chromones and Coumarins derivatives Keywords: Coumarin; Chromone; Impedance; Mustapha M. Bouhenna1,2, Mónica Vara Pérez4, Cytotoxicity. Oualid Talhi2,5, Khaldoun Bachari2, Artur M. S. Silva5, Walter Luyten3, and Nabil Mameri1*. O014 1Unité de Recherche Engineering Environnement (URIE), Ecole Nationale Polytechnique, 10 Avenue Pasteur, El Radioprotective effect of Rosemary crude Harrach, Alger, Algeria extracts (Rosmarinus officinalis L.) against 2Centre de Recherche Scientifique et Technique en Analyses Physico-Chimiques (CRAPC), BP384, Bou-Ismail, 42004, radiation induced DNA damage on Vero cells Tipaza, Algeria 3KU Leuven, Department of Biology, Division of Neurobiology Lamari-Negache. H1*, Ouafek.A1, Herrati. M2, Saadi. and Animal Physiology, Naamsestraat 59, Bus 2469, 3000 M2, Djerad.A1 Leuven, Belgium.

4Cell Death Research and Therapy Unit, Department of 1 Cellular and Molecular Medicine, KU Leuven University of Department of Medical Applications; Division of Nuclear Leuven, Leuven, Belgium. Applications; Nuclear Research Center of Algiers. 2, Bd 5QOPNA, Department of Chemistry & QOPNA, University of Frantz-Fanon BP-399 Algiers Station, Algeria. 2 Aveiro, 3810-193 Aveiro, Portugal. Laboratory of Biotechnology and Molecular Pathology; Faculty of Biological Sciences; University of Science and [email protected] Technology Houari-Boumediene. Abstract [email protected]

Oncology treatments research aim at identify and develop new anti-cancer drugs with Abstract improved efficacy and no cross-resistance to Radiotherapy is the most common modality for complement the present chemotherapeutics, and treating human cancers. However, the effective chromones and coumarins derivatives have use of ionizing radiation is compromised by the shown such activity. In this study, we test a novel side effects that result from radiation-induced series of synthetic chromone and coumarin damage to normal tissue. The use of derivatives 1-9 for cytotoxic activity against a radioprotective compounds, which can panel of tumour cell lines. selectively protect normal tissues against The anti-proliferative effects of the chromones radiation injury is of immense use because in and coumarins compounds on several tumour addition to association with protecting the cell lines (MCF-7, A549, HepG2, HTC-116, B16, normal tissue, it will also permits use of higher CaCO2 and HEK-293t) was evaluated by doses of radiation to obtain better cancer control electrical impedance spectroscopy was used to and possible cure. Our study aimed to investigate monitor cell viability in real time using the the in vitro radioprotective effect of freeze-dried CellSine® instrument, as well as their aqueous of Rosemary extract (Rosmarinus involvement in apoptosis induction were officinalis) on DNA double-stand breaks (DSBs) evaluated by flow cytometry and Western blot induced by γ-rays in epithelial cells. The cells analysis. Compound 8 significantly diminished were treated with crude extract one hour before cancer cell proliferation and viability in different exposure to gamma rays, using a source of cobalt cell lines. It induced apoptosis in a dose- 60 at doses of 0, 2, 4 and 6 Gy. After 30 mn, 1h, 4h dependent manner as shown by Western blot and 24h post exposure, γ-H2AX proteins were and flow cytometry. Indeed, compound 8 induced immunofluorescently labelled for evaluation by a early and late apoptosis from 15,9 % in non- fluorescence microscopy. The accumulation of Compound 8 treated cells to 44 %, respectively phosphorylated γ-H2AX proteins at double- between 02h and 48h at dose of 100 µM. These strand breaks (DSBs) appeared as nuclear foci. compounds showed a selectivity effect on the The cytotoxic effect of rosemary crude extract cancer cells line studied. CaCO2 and A549 cells (RCE) was also studied and measured by MTT were not affected by treatment. Compound 8, the assay. It was observed that the treatment with most potent constituent, revealed a pro-

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RCE (0,04-4 mg/ml) did not affect cell viability at interest in the system as a drug target in the 24, 48 and 72 hours after treatment. Important fields of cancers and metabolic diseases has been DNA breaks were observed in untreated cells, steadily increasing. In fact, a study carried out in and the number of γ-H2AX foci per cell increased our laboratory has demonstrated the interest of with increasing radiation dose. A pretreatment using the direct activator of AMPK, GSK 621, as a with the aqueous extract of RCE reduce the therapeutic treatment in order to induce the number of foci among irradiated cells. The death of AML (acute myeloid leukemia) blasts. results of our study suggest that the RCE could Thus, it was important to study the consequences have a role as an effective radioprotector against of the use of this direct activator on human irradiation damaging effects. However, further normal hematopoiesis and particularly on the studies are needed to verify theses results. In process of red blood cell production perspective, it is necessary to study the (erythropoiesis). Cluster of differentiation (CD) mechanism of action of phenolic compounds 34+ cells were obtained from human donors. contained in the crude extract alone or in CD34+ cells were purified from the peripheral synergy on the protection of radiation-induced blood after cytapheresis by positive selection damage as well as the study of radiosensitive and using an immunomagnetic procedure. After 7 anticancer effect of rosemary. days of culture, CD36+ cells corresponding to a highly purified population of human erythroid Keywords: Gamma radiation; γ-H2AX; progenitors were obtained by selection on Radioprotector; Rosmarinus officinalis L. immunomagnetic beads. CD36+ cells were then cultured in the presence of 2 U/mL Epo, 100 ng/mL SCF, and 10 ng/mL IL-3 up to 12 days for O015 erythroid differentiation. Our data show that, in The use of direct AMPK activators as a erythroblasts, only α1, β1 and γ1 isoforms are expressed during erythroid differentiation. In therapeutic treatment could induce the addition, we showed that AMPK (Thr172) and its apoptosis of mature erythroblasts in the bone target ACC (Ser79) phosphorylation are elevated marrow in immature (GPAlow) erythroblasts, and then decreased conjointly with the erythroid 1,2,3,4* 1,2,3,4 Meriem Ladli , Sarah Ducamp , Yael differentiation. AMPK activation in mature 1,2,3,4 1,2,3,4 Zermati , Maria-Lilia Brassart , Pierre (GPAhigh) erythroblasts through the use of direct Sujobert1,2,3, Jérôme Tamburini1,2,3, Marc Foretz1,2,3,4, activators (GSK621 and 991) induces a cell cycle Nabih Azar5, Patrick Mayeux1,2,3,4, Benoit Viollet1,2,3,4, arrest followed by the induction of autophagy Frédérique Verdier1,2,3,4 and of caspase-dependent apoptosis, whereas no such effect is observed in similarly-treated 1 Institut Cochin, INSERM U1016, Paris, France immature erythroblasts. Our work suggests that 2 CNRS UMR 8104, Paris, France 3 Université Paris Descartes, Sorbonne Paris Cité, Paris, AMPK activation during the final stages of France erythropoiesis is deleterious. As the use of direct 4 Labex GREX, Paris France, 5-Service d’hémobiologie, hôpital AMPK activators is being considered as La Pitié Salpétrière, Paris, France. therapeutic treatment in several pathologies [email protected] including cancer, this observation is pivotal. Abstract Indeed, these activators could induce the apoptosis of mature erythroblasts in the bone The 5'-adenosine monophosphate (AMP)- marrow so it will be necessary to analyze activated protein kinase (AMPK) is a hematologic parameters to prevent potential heterotrimeric complex containing α, β, and γ anemia. subunits. It plays a key role as a master regulator of cellular energy homeostasis. In order to adapt Keywords: AMPK direct activator; metabolic required energy demands, AMPK regulates diseases and cancer; primary human cellular events by stimulating ATP-generating erythroblasts cellular processes, while inhibiting ATP- consuming processes. Since AMPK inhibits cell growth and proliferation, and also promotes the regulation of Carbohydrate and lipid metabolism,

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O016 and showed a strong membrane stabilizing ability with IC of 0.5 and 0.26 ± 0.02 mg/ml, Anti-breast cancer activity, apoptosis 50 respectively. Findings of the present study induction, antioxidant potential and anti- revealed that B. dioica aqueous extract was able inflammatory effects of Bryonia dioica to inhibit growth of the MDA-MB-231 triple growing in Algeria negative breast cancer cells. Furthermore, the extract induced apoptosis and cell cycle arrest at Bachir Benarba , Soulef Zouaneb G2/M phase. Besides the cytotoxic and Laboratory Research on Biological Systems and Geomatics, apoptogenic activities, the extract possessed University of Mascara, Algeria. promising antioxidant and anti-inflammatory [email protected] activities. The latter seem to be mediated through inhibition of proteins denaturation and membranes stabilization. Overall, our findings Abstract suggest that Bryonia dioica could be considered Bryonia dioica Jacq. is a climbing perennial herb as a promising source of anti-breast cancer widely used as traditional anticancer treatment bioactive molecules. Furthermore, these molecules could be used for their antioxidant and by local populations in Algeria. The main anti-inflammatory abilities. Ongoing studies are objectives of the present study were: a) investigate the anticancer activity of the plant carried out to clarify the apoptosis-related against triple negative breast cancer, b) highlight molecular mechanisms and signaling pathways triggered by B. dioica. whether the anticancer activity would be accompanied by apoptosis induction and/or cell cycle arrest, c) evaluate the antioxidant potential Keywords: B. dioica; Breast cancer; Algeria; and estimate the anti-inflammatory activities of antioxidant; inflammation the plant. Anticancer activity of B. dioica aqueous extract against MDA-MB-232 cells was evaluated O017 using the MTT assay. Apoptosis induction and cell cycle arrest were studied using flow IMRT/VMAT as innovative technique in the cytometry. Total antioxidant capacity, DPPH treatment of cancer in radiation therapy scavenging ability and reducing power were investigated to determine the antioxidant Ismail Zergoug*, Nawel Klouch, Hakima Bahi potential of B. dioica. Moreover, human red blood cells membrane stabilizing assay and egg Clinique Oncopole l’espoir, Rue Ezzegai Elhouari, Cité 1063 AADL Hai Essabah, Bir-El-Djir, Oran, ALGERIA. albumin membrane denaturation were used to *[email protected] estimate the anti-inflammatory activity. An important time-dependent cytotoxic effect B. Abstract dioica aqueous extract was found against the Radiotherapy (RT), also known as radiation MDA-MB-231 cells. Moreover, this cytotoxic therapy, is a treatment modality based on the use effect was accompanied by induction of of high energy rays or radioactive substances, to apoptosis, and induction of cell cycle arrest at damage tumoral cells and to halt their growth G2/M phase. In fact, cells in G2/M phase and division. Modern techniques are developed increased from 15% (untreated) to 59% to get a better tumor irradiation and give fewer (50µg/ml). Besides, our results demonstrated doses to healthy tissues (Organ at risk OAR). that the B. dioica aqueous extract possessed Over the years, many techniques were employed. promising antioxidant potential. The extract The standard is the Three-Dimensional exhibited a dose-dependent DPPH scavenging Conformal Radiation Therapy (3D-CRT). This activity with an IC50 of 6.18± 0.28 (mg/ml). technique failed to achieve maximum dose to Likewise, the extract was shown to exert a dose- tumor when it is surrounded by many OARs. dependent reducing power (IC50 = 14.57± 0.77 With the advanced of technology, new mg/ml). Our findings revealed that B. dioica sophisticated techniques see light such as IMRT aqueous extract possessed an important anti- (Intensity Modulated Radiation Therapy) and inflammatory activity. Indeed, the extract VMAT (Volumetric Modulated Radiation significantly inhibited the protein denaturation Therapy). Each technique shows advantage and

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disadvantage that it is the aim of our study. 10 cultured with silibinin (0-200 μM). A patients were planned with conventional 3D-CRT proliferation assay was performed using Celigo and VMAT techniques for different cancer type imaging cytometer. Glycolysis was evaluated using Monaco V5.11 Treatment planning system using Seahorse XF96 Extracellular Flux Analyzer. (TPS) (Elekta AB (Stockholm, Sweden)). HIF1α expression was performed by Flow Objective was to choose the best PTV coverage cytometry. LDH activity and citrate levels were plan with the least OAR dose. The 2 plans were measured using LDH and citrate colorimetric compared for: PTV coverage, Homogeneity index, assay kit, respectively. Lactate levels were Conformity index and other Dose volume evaluated using lactate plus Meter Test strips. histogram (DVH) metrics related to each cancer Our results demonstrated that silibinin inhibited type. Based on DVH metrics evaluation, VMAT the growth of nasopharyngeal carcinoma C666-1 planning method was found to result in better cells in a dose dependent manner and reduced target volume coverage, improvement in dose their glycolytic activity. Treatment with Silibinin conformity and homogeneity, high doses to (100 μM) promoted metabolic reprogramming in organs at risk (OAR) were reduced when C666-1 cells. This translated in a reduction in compared to the 3D-CRT technique for different LDH activity and lactate rate and elevation in cancer type. It is locally confirmed that VMAT citrate levels after treatment. Accordingly, has great benefit in terms of OAR sparing with a expression of the transcription factor HIF1α, a great PTV coverage, but requires complex patient critical regulator of glycolysis, was down preparation, planning and verification regulated after silibinin treatment. Altogether, procedures. we show that silibinin is an effective inhibitor of NPC glucose metabolism with simultaneous Keywords: Radiotherapy; 3D-CRT; IMRT; VMAT. inhibitory effects on HIF1α. These results provide new perspective for silibinin as a O018 promising anticancer molecule for NPC. Silibinin suppresses hypoxia induced aerobic Keywords: glucose metabolism; HIF1α; NPC; glycolysis in nasopharyngeal carcinoma silibinin. Leïla Sarah sellam1*, Roberta Zappasodi2,3, Chafia

1 2,3,4,5 Touil Boukoffa , Jedd D.Wolchok ,Taha O019 Merghoub2,3,4,5, Mehdi Bourouba1 Exosomes for drug delivery to lung cancer 1Laboratory of Cellular and Molecular Biology (LBCM), Team Cytokines and Nitric oxide synthase. Immunity and pathogeny, Moufida Mahdjour 1,2,*, Soumicha Mahdjour 3, Imene Faculty of Biological Sciences, University of Sciences and Benyettou4, Keltoum Dermeche3, Jérôme kittnal1 Technology Houari Boumediene (USTHB), Bab-Ezzouar,

Algiers, 16311, Algeria. 2Ludwig Collaborative and Swim Across America laboratory, 1Biosanitary Research Institute,University Medical Center Memorial Sloan Kettering Cancer Center, New York, NY Freiburg;79106 Freiburg, Germany 10065, USA. 2Faculty of Medicine; University Djillali liabes of Sidi Bel 3Parker Institute for Cancer Immunotherapy, Memorial Sloan Abbes, BP 98, Rectorat, Sidi-Bel-Abbes, Algeria. Kettering Cancer Center, New York, NY 10065. 3University of Sciences and Technology of Oran Mohamed 4Department of Medicine, Memorial Sloan Kettering Cancer Boudiaf, El Mnaouar, BP 1505, Bir El Djir 31000 Oran, Center, New York, NY 10065, USA. Algeria. 5Weill Cornell Medicine, New York, NY 10065, USA. 4Université d’Oran 1- Ahmed Ben Bella; BP 1524 El [email protected] M'Naouer Es Sénia 31000, Es-Senia –Algerie Abstract [email protected] Tumor progression has been shown to be Abstract associated with enhanced aerobic glycolysis, Exosomes, biological extracellular vesicles (EVs) promoted by HIF1α dysregulation. In (typically 30-120 nm)1, have recently begun to nasopharyngeal carcinoma, LDH has been find use in targeted drug delivery in solid tumor associated with worse prognosis [4]. Here we research2. First, because exosomes contain investigated the effect of silibinin, a nontoxic endogenous cellular components, certain EVs flavonoid with anticancer properties [5] on may be equipped to target particular cell types glycolysis mediated in NPC. C666-1 cells were and tissues, enabling them to overcome

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biological barriers. Secondly, owing to their O020 endogenous origin, exosomes are less likely to be DNA damage and mitochondria-mediated immunogenic or cytotoxic than other synthetic delivery systems. Finally, the lipid bilayer of apoptosis of colorectal carcinoma cells exosomes may protect the drug from rapid blood induced by scorpion venom components clearance, and may reduce cytotoxicity Louisa Béchohra1*, Christian Gaiddon2, Fatima associated with off-target drug effects3. In this 1 1 study, we investigated the effectiveness of two Laraba-Djebbari and Djelila Hammoudi-Triki . different populations of EVs (microvesicle- and 1. USTHB, University of Science and Technology Houari exosome-enriched) as carriers of Doxorubicin to Boumediene, Laboratory of Cellular and Molecular Biology, lung cancer cells. EVs were isolated from lung Bab Ezzouar 16111 cancer cell cultures using differential 2. U1113 INSERM, 3 Avenue Molière, Strasbourg 67200, centrifugation and characterized by electron France. microscopy, nanoparticle tracking analysis, and louizabechohra@hotmail. fr; [email protected] Western blot. The uptake of microvesicles and Abstract exosomes by the A-549 was assessed by flow cytometry and confocal microscopy. The EVs Cancer is one of the leading causes of death in the were loaded with Doxorubicin and the world and synthetic chemicals used in effectiveness of EV-mediated drug delivery was chemotherapy and radiotherapy chemicals have assessed with viability assays. The distribution of extremely high cytotoxicity and cause much EVs and EV-delivered Doxorubicin in cells was collateral damage to normal tissues. Several inspected by confocal microscopy. Our main studies have established that venom peptides finding was that the loading of Doxorubicin to from several scorpion species induced cytotoxic, lung cancer cell-derived EVs increased its antiproliferative and apoptogenic effects on cytotoxic effect. This capacity was independent of tumor cells in vivo and in vitro. Therefore, the the EV population and the cell line tested. purpose of the present study was to investigate Although the EVs without the drug increased the antitumor mechanism of F3 fraction isolated cancer cell viability, the net effect of enhanced from Androctonus australis hector venom on cytotoxicity remained. Both EV populations colorectal tumor cell line HCT116. The cell delivered Doxorubicin to the recipient cells viability and cell proliferation were analyzed by through endocytosis, leading to the release of the MTT and clonogenic survival assays. DNA drug from within the cells. The removal of EV damage was assessed by comet assay and surface proteins did not affect exosomes, while western blot analysis. Apoptosis was evaluated the drug delivery mediated by microvesicles was by Hoechst 33258 staining and protein partially inhibited. Cancer cell-derived EVs can expression of cleaved caspase 3 and PARP1 along be used as effective carriers of Doxorubicin to with assessment of the TP53 dependent their parental cells, bringing the drug into the apoptosis target genes by RT-Q-PCR. Obtained cells through an endocytic pathway and results revealed that F3 fraction induced a increasing its cytotoxicity. However, due to the significant decrease in cell growth of colorectal increased cell viability, the use of cancer cell- tumor cells associated with a DNA damage effect derived EVs must be further investigated before particularly double strands break due to any clinical applications can be designed. phosphorylation of histone γH2AX. The F3 fraction promotes cells apoptosis by up-regulated the expression of tumor suppressor gene TP53 Keywords: Drug delivery; Exosome; Lung cells; associated with direct transcriptional activation Microvesicles; Doxorubicin. of the pro-apoptotic PUMA, NOXA, BAX and BAD, and simultaneously down-regulated the level of the antiapoptotic BCL-2 gene expression. In addition, high amounts of cleaved caspase 3 and its substrate the poly (ADPribose) polymerase 1 (PARP-1) were detected, reflecting the DNA repair inhibition in favor of a caspase dependent apoptosis. Our investigations reveal the presence of antitumor peptides endowed with genotoxic

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and apoptotic activities in F3 fraction. The (mucinous / serous) are the most common. identification of these venom‐derived peptides in Currently, there is no specific test to detect interface with a transcriptome‐centric ovarian cancer at an early stage because of the mechanistic study approach will be the broad deep and intrinsic location of the ovary. In view utility to overcome the resistance of conventional of this study, early detection should be chemotherapy. considered in order to avoid late diagnosis of malignant or benign primary or secondary Keywords: F3 fraction, H2AX, Apoptosis, TP53. ovarian tumors to ensure adequate management.

Keywords: Ovarian cancer; screening; early O021 diagnosis; sensitization. Pathological and epidemiological features of

ovarian carcinoma (serous / mucinous) in O022 western Algeria Nanoparticulate Drug Delivery System for Sonia Seddiki 1, Hiba Bouchikhaoui2 , Hiba Rmmas1, Chemotherapy Rahma Saidani1, , Tewfik Sahraoui1 Sarah Bouameur 1*, Fatma Zohra Ghanassi2 1Affiliations 1. Laboratory of Developmental Biology and Differentiation. Faculty of Science of Nature and Life. 1Department of Pharmacy University of Djillali Liabes –Sidi University of Oran 1. Ahmed Ben Bella Bel Abbes, 22000, Algeria. 2Affiliations 2. Laboratory of Pathology. EHU. University of 2Department of Pharmacy, University of ALGIERS 1, Algiers, Oran 1. Ahmed Ben Bella 16000, Algeria. [email protected] [email protected] Abstract Abstract Ovarian cancer has become one of the most The emergence of the chemoresistance of common gynecological cancers after breast cancerous tumors and virus propels the medical cancer and the second leading cause of cancer research towards two ways: the development of death in women. This public health problem is new Active Pharmaceutical Ingredient and becoming more and more important because of vectorization. This latter, exploiting only the the increased life expectancy. Currently, one in physicochemical properties of the nanovectors, ten women is at risk of developing ovarian has the advantage of targeting the therapeutic cancer in her lifetime. The early diagnosis of all sites and considerably reducing toxic effects. ovarian tumors and the sensitization of women Thus, since the marketing in 1990 of towards its tumors is important because the Amphotericin B in liposomal form "Ambisome®", profound situation of the ovary and the non- various nanocarriers have been developed both specific signs often explain a late diagnosis, on for therapeutic and imaging, as example: the other hand, to date, no favorable impact of nanoliposomes, polymersomes, dendrimers, the screening. Mortality due to ovarian cancer fullerenes, nanotubes , nanoshells, micelles, has been demonstrated. Our work was carried quantum dots, nanocapsules, nanospheres, out at the level of the pathology department of nanovaccines, and nanocrystals ... In our work, the university hospital in Oran. The aim of our we are interested in lipid nanoparticles, because work was to conduct an epidemiological and of their biocompatibility, their biodegradability pathological study to identify the most common and their stability in biological medium. We will risk factors in ovarian cancer patients over a share our experience in encapsulation of Acylic period of one year. The results of our study Nucleoside Phosphonate for antiviral and anti showed that the occurrence of ovarian cancer tumoral treatments. increases proportionally with age. The highest frequency was observed for women aged 45 to Keywords: SLN, HBV, HIV, Compritol, Adefovir 59 years. Which coincides with menopause. 80% of sick women are married, most of whom have had their first pregnancy at a late age (after 25 to 30 years) or are hypofertile. Epithelial tumors

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O023 inhibition of the FA/BRCA pathway to enhance the efficacy of chemotherapy in HCC. Role of BRCA1 gene in hepatocellular carcinoma chemoresistance Keywords: BRCA1, chemosensitization, hepatocellular cancer, interstrand cross-linke Sana Ferroudj1, Massinissa Yahia1 drugs. 1Faculty of nature and life Sciences, Abbès Laghrour

university, Box 1252 Batna street Khenchela -40004 Khenchela-Algeria. O024 [email protected] In vitro anticancer activity of Ephedra vulgaris Abstract against different human cancer cell lines Hepatocellular carcinoma (HCC) is one of the Soumicha Mahdjour 1,2,*, Nuria Mut Salud 3, Fatima most lethal malignant tumors for which 4 5 therapeutic options are limited. The failures of Bendidouche , Ko ko Myo conventional and targeted therapies in the 1Department of Organic Chemistry, Faculty of Sciences, treatment of CHCs are due to the highly University of Granada, 18071, Granada, Spain resistance of these tumors which is associated 2Department of Biotechnology, University of Sciences and with DNA repair genes. Our attention has been Technology of Oran Mohamed Boudiaf, BP 1525, EL focused on studying the potential role of BRCA1 M'Naouer, Oran, Algeria 1. in the chemoresistance of CHCs. Response of HCC 3Institute of Biopathology and Regenerative Medicine (IBIMER), University of Granada, Armilla, Granada, 18100, cells to interstrand cross-links was investigated Spain. by cell viability assay following exposure to 4Department of Chemistry, University of Sciences and mitomycin C, cisplatin, and melphalan. BRCA1 Technology of Oran Mohamed Boudiaf, BP 1525, EL protein was tested by Western blotting. Effects of M'Naouer, Oran, Algeria 5 BRCA1 ectopic expression were studied in HepG2 Department of Chemistry, University of Mandalay, 100101, Mandalay , Myanmar cells with BRCA1-expression plasmids. Effects of [email protected] BRCA1 downregulation were studied in SNU449 cells with BRCA1-specific siRNAs. Response of Abstract transfected SNU449 cells to mitomycin C was “Ephedra vulgaris” belongs to the Ephedraceae analyzed by cell viability tests and cell cycle family. It is a species of Ephedra that grows analysis using flow cytometry. Expression of mostly in the desert. Ephedra species have a long Fanconi anemia and double-stranded DNA break history in traditional Chinese medicine repair genes was significantly upregulated in (approximately 5000 years), with uses in the HCC tumors. This upregulation displayed a treatment of allergies, nasal congestion, gradual amplification during tumor progression. bronchial asthma, coughs, and flu. The aim of this BRCA1 and BRCA2 genes were among study was to evaluate the cytotoxicity and consistently upregulated genes. Epithelial-like apoptotic/antigenotoxic effects of Ephedra as HCC cells had low BRCA1 expression and low well as its mechanism. Five different varieties of chemoresistance, whereas mesenchymal-like Ephedra were prepared in the traditional way HCC cells had high BRCA1 expression and and then freeze-dried. Phytochemical screening increased chemoresistance. Ectopic expression of tested for the presence of secondary metabolites BRCA1 increased the chemoresistance of using standard procedures and antioxidant epithelial-like HepG2 cells. Conversely, BRCA1 activities. The cytotoxic activity was evaluated on knockdown chemosensitized mesenchymal-like breast cancer (MCF-7), prostate cancer (PC-3), SNU449 cells. Chemosensitization of SNU449 colon cancer (T-84), lung cancer (A-549), and cells was due to cell cycle arrest at 4N stage. Our human peripheral blood lymphocytes via a MTT study shows that enhanced expression of the assay. The pro-apoptotic effects were evaluated BRCA pathway genes is an important mechanism with Anexin V/Propidium Iodide and western involved in HCC chemoresistance. Moreover, we blot of Bax, Bcl-2, TP53, and TP73. Induction and demonstrated that such chemoresistance can be reduction of ROS were assessed by fluorimetry. overcome, at least partially, by downregulating Genotoxic and antigenotoxic effects were just one BRCA pathway gene, such as BRCA1. We evaluated with a comet assay and micronuclei on believe it is worthwhile to further explore the binucleated cells. Four of Five Ephedras had

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cytotoxic effects against MCF-7 and A-549 while apoptosis via a p53/p73-mediated mechanism. not affecting normal cells. These Ephedras induce The Ephedras present antigenotoxic properties, cell death by apoptosis modulated by p53/p73. which may be related to the antioxidant capacity. In CHO-K1 cells, the Ephedras decrease the Future studies on Ephedra components are damage induced by hydrogen peroxide and necessary to understand the interactions and Mitomycin C measured in the comet and beneficial properties. micronucleus assay respectively. The IC50 range of effective Ephedras in MCF-7 was 17 to 102 Keywords: Ephedra; Cancer; μg.mL−1. This effect may be related to its use in Antigenotoxicity; Apoptosis. traditional medicine. On the other hand, immortalized Chinese hamster ovary cells (CHO- K1) and lymphocytes did not show a cytotoxic effect. The most potent Ephedra induced

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Cancer diagnostic technologies, Immunotechnology and Cancer Immunotherapy

48

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O025 chemotherapy. We observed increased levels of DNA damage in patients with lymphoma, as The use of Comet Assay as a valuable tool for compared with those in healthy controls. We assessing DNA damage in Algerian children suggest that the direct effect of chemotherapy, with lymphoma the alteration in redox balance may have an influence on oxidative / antioxidant status. 1,* 2 3 Assia Galleze , Abdurrahim Kocyigit , Nacira Cherif , However, further studies of the role of ROS in 4 1 Nabila Attal , Chafia touil Boukkoffa , tumor biology may lead to new strategies for Rachida Raache1 cancer therapy.

Department of Cellular and MolecularBiology, Cytokines and NitricOxideSynthases, Faculty of Biology, University Houari Keywords: Chemotherapy; Comet assay; DNA Boumediene USTHB, Algiers, Algeria. damage; lymphoma. BezmialemVakifUniversity, Faculty of Medicine, Department of Biochemistry, Fatih, Istanbul, Turkey HematologyDepartment, Beni Messous Hospital, Algiers, O026 Algeria Department of Immunology, Institute Pasteur of Algeria, Molecular interactions between anticancer Algiers, AlgeriaAffiliation [email protected] drugs and their target cells and their risk against healthy cells, cases of leukaemia and colorectal cancer in patients in the Abstract departments of oncology and hematoclinic at Chemotherapeutic agents used to inhibit cell the University Hospital Centre in Tlemcen, division and reduce tumor growth, increase Algeria reactive oxygen species levels, which contributes 1,2, 3 to their genotoxicity.The comet assay is Moustafa Sellah *, Boudaoud Imane , Belkadi 4 expensive and rapid method to detect the Chaima damage at cellular levels and has been used in various cancer populations undergoing Faculty of Medicine, University of Abou Bekr Belkaid of Tlemcen, Algeria 1. chemotherapy. The present study aim to National Institute of Advanced Training of Midwives in determinate the association between Tlemcen, Algeria 2. chemotherapy and DNA damage in Algerian Service of Paediatric, Establishment of Mother and child, children with lymphoma. For our study, we Tlemcen, Algeria 3. Hospital of Remchi, Tlemcen, Algeria 4. selected thirty children with lymphoma treated [email protected] in university hospital of Beni Messous (Algeria), and fifty unrelated subjects as controls, after obtaining the informed consent in accordance Abstract with the declaration of Helsinki (1964). Cancer chemotherapy is a therapeutic method Mononuclear leukocytes were isolated using using all chemicals, drugs, plant extracts (natural ficoll histopaque 1.077 (Sigma) and DNA damage compounds) or chemically synthesized was analyzed by alkaline comet assay.The results laboratory products, toxic for cancer cells, aimed were analyzed using Comet Assay IV program. at blocking cell proliferation in a phase of the cell (Perspective, England). DNA damage was cycle or to prevent it from progressing in the significantly higher in lymphoma patients before cycle (cytostatic) or killing the cell (cytotoxic). and after therapy than in healthy controls (10.16 These substances are even assimilated to poisons ± 2.83 vs 2.58 ± 0.43 (P < 0.001); 21.14 ± 7.64 vs of the cell cycle. This explains the name still used 2.58 ± 0.43 (P < 0.001), respectively).This abusively antimitotic. It represents the only damage maybe related with excessive ROS theoretical way to control the spread of cancer as generation and insufficient antioxidant capacity. it is carried by the blood; it circulates throughout Indeed, the chemotherapeutic agents generate a the body while surgery and radiotherapy is only high level of ROS and can oxidize lipids, for localized areas. In order to evaluate the real hydrolyze amide bonds of proteins and cleave situation in terms of anticancer molecular RNA and DNA molecules. Our study is the first to interactions used in chemotherapy, we carried apply the Comet Assay for assessment of out a study at the level of oncology and genotoxicity in Algerian children undergoing

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hematoclinic services at University Hospital O027 Centre in Tlemcen. Our study was carried out at The role of mammography, incidence and risk the level of the oncology and hematoclinic services of the University Hospital Center (CHU) factors at patients with breast cancer Dr. Tidjani-Demerdji of Tlemcen. In this work, we Maamar Boukabcha1,3*, AichaBadaoui2, Abd-El-Kader followed a methodology based on the use of 3 questionnaire and interview of the staff of the Ali Benamara two services of oncology and hematoclinic Department of Biology, Faculty of natural and life sciences, (doctor pharmacist and nurse) and taking Hassiba Benbouali University of Chlef, Algeria. information on the patients starting from an Hospital public Establishment Bedje Sisters of Chlef, Algeria. interview and their medical records for 5 Laboratory of Theoretical Physics and Physics of Materials, months, from November 2017 to March 2018. Hassiba Benbouali University of Chlef, Algeria. The results obtained from this study provide [email protected] information on 10 patients, including 06 patients in the oncology department followed by cancer of Abstract the colon and rectum with a frequency of 50% Breast cancer is a major public health problem in for each and 04 patients in the hematoclinic the world, is the most common cancer at women service followed only by leukemia. However, and describes it as rare at men .The objective of mortality only affected one patient with rectal our work is to estimate the different incidence cancer. The action of anticancer agents against rates of breast cancer and also to know the risk colorectal cancer target cells was studied for two factors and role of mammography for this anticancer drugs, cetuximab and bevacizumab. disease at women and men. Our study is carried The first is in the inhibition of the EGF pathway out on patients who have breast cancer after by binding this molecule to the EGFR receptor, diagnosis by specialist doctors according to the however, the second directs against vascular medical records are treated at the level of Chorfa endothelial growth factor (VEGF). While, polyclinic oncology service in the Chlef region.We anticancer interactions against healthy cells based on the period of January 1st, 2010 and up represent poor interactions manifesting as toxic to December 31st, 2013 in our study and hospital effects, secondary or undesirable for patients. public Establishment Bedje Sisters of Chlef in the The two types of cancer studied have different same study region. We have used more than 150 interactions, some of which are common, for breast cancer patients in the HPE Bedje Sisters of example: abdominal bloating, decreased GB, GR Chlef. The gross incidence rates of patients and platelets. The cancer treatments during 04 years from 2010 to 2013 of Chorfa theoretically act specifically on the mechanisms Polyclinic are 19.88 woman patients per 100 000 of oncogenesis and aim to eliminate target cells. inhabitants and 0.51 man patient per 100 000 They act either locally, that is to say only on the inhabitants. The mammography plays a very cancerous cells of an affected organ, or generally, important role in diagnosis and screening that is to say on all the cancerous cells present in according to our study. This pathology is caused the body, but not are not without side effects. by several risk factors such as tobacco, alcohol, These anticancer drugs are taken in the long run, the pill, asbestos, radiation, electromagnetic and the cumulative effects of adverse effects can fields ... etc. Finally, it is necessary to reduce the be harmful to the quality of life of the patient. exposure to risk factors to fight against breast This phenomenon can result in a lack of patient cancer by prevention, early diagnosis and adherence to its treatment. In addition, the mode positive treatment as recommendations. of action of these anticancer drugs is not specific only against their target cells but they also react Keywords: Diagnosis, Breast Cancer; Incidence; against healthy cells. Risk Factors; Chlef region. Keywords: Cancer of colon rectum, leukemia, Anticancer, Molecular interactions.

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O028 urothelial carcinoma were the most predisposed to urinary tract infections. The Urinary tract infection in Algerian patients cytobacteriological examination shows that 3 with bladder cancer: Histopathology and germs dominate among the uropathogenic germs etiology namely Escherichia coli (44%), Klebsiella sp (14%), followed by Enterobacter sp (10%). In 1, 2* 1 Hassina Khaldoun Oularbi , Kenza Merabet , conclusion, since multi-resistant bacteria are 1 1 1 Safaa Rebahi , Naima Ouraghi , Sarah El Mokhfi , now a major health problem, periodic monitoring Aouda Aniour1 by prescription of ECBU remains essential in these patients. 1Departement de Biologie et Physiologie Cellulaire, Faculté des Sciences de la nature et de la vie, Université Blida 1, Algérie. Keywords: Bladder cancer; Histopathology; 2Laboratoire d'Ecobiologie Animale, école Normale Urinary infection; Uropathogenic germs. Supérieure, BP 92, 16050 Kouba, Alger, Algerie [email protected] Abstract O029 The five most common infection-associated Expression of human serum TCTP in prostate cancers in North Africa in order of incidence are cancer is correlated with the agressivity of bladder cancer, cervical cancer, liver cancer, cancer stomach cancer, and nasopharyngeal carcinoma.The incidence of bladder tumours Asma Bourefis1, Hajira Berredjem1, Omar Djeffal2 and grows with age and is 2-3 times more frequent in Palma Rocchi3 men this frequency is due to various factors, mainly tobacco, and occupational exposure to 1 Université Badji Mokhtar-Annaba, Faculté des Sciences, toxins. Our prospective multiparametric study, Département de Biochimie, Laboratoire de Biochimie et de MicrobiologieAppliquées,Algérie covering the period from 2007 to 2018, involves 2CabinetMédicald’Uro-Chirurgie, Annaba, Algerie 600 bladder cancer patients admitted to different 3Centre de Recherche en Cancérologie de Marseille, Inserm, urology services of Blida (39%) (Center Anti Institut Paoli-Calmettes et Aix Marseille Université Marseille, Cancer and CHU Frantz Fanon) and Algiers France (61%) (CHU Ain Naadja and CHU Mustapha [email protected] Pacha). In this study we first investigate the risk Abstract factors for bladder cancer (smoking and Prostate cancer (PCa) is a major healthcare occupational exposures), its histological problem. Clinical outcome at diagnosis is evolution (tumor grade), and its incidence in heterogeneous and not easy to predict; thus, hospitalized patients. Then, we identified the predictive and diagnostic markers are needed. prevalence of urinary tract infections (UTIs), TCTP (Tumor Controlled Translationally Protein) offending germs and the evolution of their also known as p23 is a growth-associated protein resistance to antibiotics. Our results show a clear present in different types of tissues with male predominance whatever the year. However, preferential expression in mitotically active the percentage of women has increased tissues. On the other side, tumor reversion and significantly in 2010 (12%) reaching a maximum malignant transformation have been associated of 21% in 2018. The percentage of smokers is with TCTP. The Palma Rocchi team has shown an much higher with a rate of 72%. However, Grade overexpression of TCTP in castration-resistant 2 (27%), G1 (20%) and G3 (10%) in smokers prostate cancers. In this work, we evaluated in predominate compared to non-smokers patients subjects with PCa, the serum concentrations of G2 (15%), G1 (15%) and G3 (5%), respectively. the TCTP antigen and its association with tumor In the age groups over 50 years, we observe an aggressiveness. ELISA was used to quantify increase of UI occurrence from 3% in 2007 to serum TCTP concentrations in 58 PCa patients 39% in 2018. The current findings agree with and 50 healthy individuals. Serum TCTP levels other published studies witch demonstrated a were significantly higher in patients with PCa dose-response relationship between tobacco and (417.798 ± 381.88 ng/ml) than in control bladder cancer [5, 6]. In addition, we subjects (103.383±58.57)(P < 0.0001).The demonstrated that patients with grade 3 results showed an over-expression of TCTP in

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tumors and indicated that malignant tumors Silico bioinformatics study was established. expressed higher TCTP concentrations than Then, using real-time PCR, we explored on new benign tumors. Thus, in the case of non- samples the G12D mutation that was previously aggressive cancer (Gleason score< 7) a found and associated with our patients. Thus, we significant increase in secreted TCTP protein determined in the first part of our work a single (257.549±262.11 pg/ml) is recorded compared type of mutation at codon12 (G12D mutation) to that observed in the case of aggressive PCa relating to the change of the amino acid Glycine (Gleason score ≥ 7) (538.824 ± 463.08 pg/ml) (P to Aspartic Acid. Due to transition from guanine < 0.0001). Concentration values of TCTP were to adenine, (G>A) with a rate of 16%. This rate is also correlated with the Gleason score. Elevated up to date with recently reported figures, which levels of TCTP antigen in human serum occur in in turn show that mutations in the KRAS gene are PCa and are related to the prostate cancer found in 15 to 30% of pulmonary malignancy level suggesting its use as a adenocarcinomas. To add, we determined that diagnostic biomarker. these mutations are found only on Codon12, given that more than 82% of KRAS gene Keywords: Prostate cancer, TCTP, Biomarker, mutations are found at Codon12. With regard to aggressive cancer. the In Silico study, the latter predicted that the G12D mutation is rather tolerant; hence, this suggests that this substitution does not alter the O030 function of Ras protein. This mutation sits at a Exploration of exon 2 of the KRAS gene in region between the analyzed species that is not conserved. This indicates that the G12D mutation Non-Small Cell Lung cancer (NSCLC) does not once again alter the protein structure found in the different species since it maintains Noria Bouras 1,2*, Abdelkader Bousahba3, Ahlam its role in the signaling pathways. The second Megaïz3, Florence de Fraipont 4, Tewfik Sahraoui1, part of our work was performed on only 17 Fatima Zohra El Kebir1 NSCLC patients where real-time PCR results

1 Laboratoire de biologie du développement et de la revealed a single non-smoking male patient with différenciation (LBDD), Université Oran 1 Ahmed Ben Bella, G12D mutation. This work will allow selecting Oran, Algérie. patients with mutational status to direct them to 2 Département de génétique moléculaire appliquée (GMA), more targeted therapies Université des sciences et de la technologie d’Oran (USTO- MB), Algérie 3 Service d’oncologie médicale, Centre hospitalo-universitaire, Key words: functional mutation G12D, KRAS, CHU d’Oran, Algérie non-small cell, lung cancer. 4 Unité médicale de génétique moléculaire : maladies héréditaires et oncologie, Centre hospitalo- universitaire, CHU de Grenoble Alpes (CHUGA), France O031 [email protected] Abstract Epidemiological study and role of zinc and lead on Breast Cancer in El Oued (Algerian) Non-Small Cell Lung cancer (NSCLC) accounts for Population nearly 85% of all Bronchial cancers. Among various genes involved in the appearance of this Samir Derouiche*1, Naouel Atoussi1, SafaGuediri1 cancer, the proto-oncogene KRAS represents the gene most frequently mutated with a proportion Department of Cellular and Molecular Biology, Faculty of of more than 25%. KRAS is followed by other natural sciences and life, El-Oued University. oncogenes, such as EGFR, NRAS, BRAF, and [email protected] PIK3CA. Some mutations can affect these genes Abstract and are associated with both the histological type and smoking history of NSCLC patients. The aim Cancer is a disease characterized by abnormal of our work was to first look for the mutational cell growth localized in a specific place or can status of exon2 of the KRAS gene covering 35 invade other parts of the body. Cancers cells can patients with adenocarcinoma NSCLC by form a tumor which invades the local tissue. pyrosequencing. In order to predict the impact of Cancer has become the leading cause of death. the mutation found within the Ras sample, an In Breast cancer is a major malignant tumor for

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women with approximately 23% percent. Its increase in the release of reactive oxygen species, frequency is very high for developed countries species that are very sensitive to cellular specifically. It is very difficult to determine the constituents including the proteins involved in cause of breast cancer. Exposure to DNA repair, which may contribute to the environmental pollution (such as pesticide and disappearance of cancerous cells. Hence heavy metals) and lifestyle play an important epidemiological studies are needed to role in the etiology of this disease.The aim of this understand the variations and the occurrence of study is to analyses the epidemiological and breast cancer. This study also revaluated appreciate the distribution of breast cancer and environmental contamination by lead is a very to evaluate the effect of zinc and lead in women important risk factor for breast cancer expected breast cancer in El Oued population. Our in the region of El Oued. Moreover, The low Zinc epidemiological study was carried out on 1505 level in breast cancer patients, indicates the cancer patients in the period (2007-2017) of the importance of diet factors and zinc deficiency in El Oued region. For mineral study, This work is breast cancer expected. carried out on 36 volunteers women, were divided into two groups; a group of 20 healthy Keywords: Breast; Cancer; Epidemiology; Zinc; control women and 16 women has breast Lead; El Oued. cancer,on which we made the dosage of zinc in serum and hair, lead in serum and lead in the soil of different regions of ElOued. O032 In Results for 316 cases of breast cancer Effect of glucose oxidase on the cytokine recorded, 28.95% patients were aged between profile and cholesterol of monocytes in liver 41-50 years. In the other hand, 1.9% of cancer patients are men versus 98.1% are women. In cancer this study, 48% of the breast cancer patients had Souad Zoudji, Mourad Aribi and Mustapha Haddouche a tumor affecting the right breast whereas 52% of the people were affected in the left breast. In Laboratory of Applied Molecular Biology and BIOMOLIM, our study, compared to controls, serum Zn levels Department of Biology, University of Abou-Bekr Belkaid, had significantly low (p < 0.05) in breast cancer Tlemcen 22,AbiAyedAbdelkrim street, BP 119, Faubourg patients which agreement with result of HONG et Pasteur, 13000 Tlemcen, Algeria. *[email protected] al., (2006) study [5], Zinc level in the serum of patients with cancer is lower than healthy individuals has been shown in other Abstract epidemiological study [6]. Our study also found that there was a lower hair zinc level (p < Cancer has an extremely important human and 0.01) in the breast cancer patients, which is in socio-economic impact. It is due to several agreement with the hypothesis of the decrease of cellular disturbances causing uncontrollable zinc level in hair of the patients has a cancer by proliferation, which stimulate immune system an increased consumption compared to the elements including monocytes that can play a healthy peoples [7]. Zinc is an essential element dual role either in the elimination or progression of life, which is of specific functional importance of cancer cells (Elliott et al., 2017), these in various physiological systems [8]. Zinc also disturbances affect a variety of functions such as plays a role in cellular signaling such as the glucose metabolism (Annibaldi and Widmann, phosphorylation cascade of MAPK and Akt 2010). This work hopes to investigate the effect required for cell development under normal of glucose oxidase at the level of the monocyte on conditions or in pathological cases such as cancer the tumor growth thus determining its impact on [9].In our study, lead levels had significantly high the glycolysis activity and on the mitochondrial (p < 0.001) in serum of breast cancer patients metabolism. The aim of this study is to show the compared to controls. There is not much role of GOD in the polarization of monocytes in evidence on the carcinogenic aspect of lead in contact with tumor cells. Monocytes isolated humans. but lead can cause cancer by a genetic from the blood of the cancer patient were co- aspect through direct interaction with DNA by cultured with the tumor epithelial cells isolated experience in a group of workers exposed to from a biopsy of the liver cancer, in a culture lead, this interaction appears to be due to an medium supplemented or not with GOD. GOD

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induced a decrease in the INF-γ and increase of investigated the CCL3L1 CNVsin relation to AS IL-10 as well as in NO and Arginase in the risk disease. It reveals that CCL3L1 CNVs presence of Glucose oxidase compared to those maybeinvolved in susceptibility to AS risk in the without GOD. In conclusion, our results showed Algerian population.These results need to an anti-inflammatory effect was reported in beconfirmed in larger and other ethnic monocytes in contact with liver tumor populations. epithelialcells. Keywords: Algerian population, ankylosing Keywords: Liver cancer, monocyte, GOD, spondylitis, CCL3L1 gene, copy number Cytokines. variations.

O033 O034 Association study of copy number variants in Laboratory testing requirements for CCL3L1 gene with risk of ankylosing diagnosis of multiple myeloma spondylitis in a West Algerian population Ouadi Ibtissem1, Pr .H. Meriche 1, S. Gadiri-Meriche1, N Dahmani Chahinez Amira1*, Benzaoui Ahmed2, Grifi2 Amroun Habiba3, Zemani-Fodil Faouzia1, Petit- 1Faculty of Medicine, Badji Mokhtar University, Laboratory of 4 1 Teixeira Elisabeth , Boudjema Abdallah . ClinicalImmunology, Saint-theres teaching hospital,Annaba, Algeria. 1Laboratoire de Génétique Moléculaire et Cellulaire/ 2Faculty of Medicine, Badji Mokhtar University, Department Université des Sciences et de la Technologie d’Oran Mohamed of Hemmatolology, Saint-theres teaching hospital,Annaba, Boudiaf (USTO-MB), BP 1505 EL-M’NAOUAR-31000 ORAN- Algeria. Algeria *[email protected], [email protected] 2 Service de Rhumatologie/Centre Hospitalo-universitaire d’Oran (CHUO), Algeria 3 Laboratoire d’Immunogénétique et de Transplantation, Département d’Immunologie, Institut Pasteur d’Algérie à Abstract Alger, Algeria 4 GenHotel, UnivEvry, Université Paris-Saclay, Evry, France Multiple myeloma is a neoplastic disorder *[email protected] characterized by proliferation of a single clone of plasma cells derived from B cells. This clone of plasma cells proliferates in the bone marrow and Abstract frequently invades the adjacent bone, [1]. Monoclonal immunoglobulins are markers Numerous single nucleotide polymorphisms ofplasma cell proliferative diseases and have (SNPs) were explored in the Algerianpopulation been describedas the first (and perhaps best) to evaluate associated ankylosing spondylitis serological tumor marker.. Panels of tests have (AS) genetic risk factors, butno study has been recommended for sensitivity and identified the impact of copy number variations efficiency[2]. The aim of this study is to discuss (CNVs). The aim of thestudy was to determine theuse ofvarious tests such as protein whether CNVs of CCL3L1 gene werealso electrophoresis and immunofixation associated with the susceptibility of AS disease. electrophoresis as well as immunoglobulin The dataset of the current study is composed of quantitation, free light chain quantitation, and 80 patients with AS and 100 healthy controls.All heavy-lightchain quantitation by immuno- samples were genotyped by digital droplet PCR nephelometry and To determinethe clinical and (ddPCR). Chi‐square test and ORcalculation were laboratory features of patients with diagnosed used to evaluate association between CNVs and MM. Records of all patients in whom multiple AS and the riskassociated with copy numbers myeloma was initially diagnosed at department (CN). An association between high CCL3L1 copy of hematology, from January 1, 2010, to numbers and susceptibilityto AS was found (p = December 31, 2016, were reviewed. The .02, OR = 3.17 [1.47–6.82]). Our result shows that detection of M-proteins relies on the ability CNVs of CCLL31 gene seem to play a role in the todifferentiate between monoclonal and susceptibility to AS disease in the Algerian polyclonal immunoglobulins was by agarose gel population. This is the first study that assays. In addition, IFE in agarose zone 54 Oran 05-07 December

electrophoresis was used to characterize the immunoglobulin heavy and/orlight chain isotypes. Nephelometric methods can aid in thedetection of M-proteins and can complement some of theweaknesses of electrophoretic techniques. Of the 172 study patients, 18% were younger than 50 years, and 25% were 70 years or older. The median age was 61 years. Of these 172 patients, 50% were men. Anemia was present in 34% of patients, bone pain was present at diagnosis in 75% of patients. Serum protein electrophoresis revealed a localized band in 82% of patients, immuno-fixation showed a majority of the myeloma proteins (50%) were of the IgG isotype followed by IgA(23%), whereas light-chain myeloma was present (18%), IgM (9%). .A monoclonal light chain was found in the urine in 54%. The age and sex distributions of our patients were similar to those of patients with multiple myeloma seen in Tunisia and Moroco.Clinical history was taken in all our patients and the major presenting complaints were recorded. Bone pain was one of the chief complaints in 75 % of our patients. In studies by Gupta and Kyle , 79% and 58% patients respectively had bone pains at diagnosis. Generalized weakness and fatigability were recorded in 34% of our patients and in 32% patients in a study of the Mayo clinic. Similarly, raised serum creatinine levels were found in 50% of the patients, for who these results were available, compared with only 55% and 48% patients in two studies by Kyle and al. MM accounts for about 1% of all types of malignancy and slightly more than 10% of hematologic malignancies. The reported increased incidence during the past few decades is probably related more to the increased availability of medical facilities for elderly patients and to improved diagnostic techniques than to an actual increased incidence. A better understanding of the biology of myeloma means that quite soon we might be able to define and tailor-make at diagnosis which patients will most readily respond to which biological treatments.

Keywords: M-protein; immunofixation; multiple myeloma; protein electrophoresis.

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Poster

Communications

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Oncogenomics and Pharmacogenomics

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P035 epidemiology and histopathology of CNS cancers in Western Algeria reported that the incidence of Epidemiological and Histopathological these malignancies is alarmingly increasing. Assessement of Central Nervous System Cancers in a Northern African Population: A Keywords: Brain cancer; CNS; North Africa; Restrospective Study over Seven Years Epidemiology.

Walid Hassan HAMRI1, Mohammed Abdessamed TOUATI 2, Noria HARIR3 P036 Department of Biology, Djillali Liabes University of Sidi bel Abbes, Ex ITMA, rectorat de l'Université de sba route de Polymorphism of the Cytidine Deaminase Tlemcen bp55,22000, Algeria. Department of Biology, Djillali Liabes University of Sidi bel (CDA) Gene and Response to Nucleoside Abbes, Ex ITMA,rectorat de l'Université de sba route de analogs-based Chemotherapy: The Value of a Tlemcen bp55,22000, Algeria. Phenotypic Test Laboratory of Molecular Microbiology, Proteomics and Health, Algeria Adel Gouri 1* , Bachir Benarba 2 , Mohamed Chefrour [email protected] 3 , Joseph Ciccolini 4 , Samia Benyahia 1 , Sadek Benharkat 1

Abstract 1 Laboratoire de Biochimie, CHU Annaba, Faculté de Médecine, Annaba, Algérie Brain cancer, which is one of the most important 2 Laboratory Research on Biological Systems and Geomatics, central nervous system (CNS) cancers as its Department of Biology, University of Mascara, incidence is increasing in the world, has attracted Algeria. much attention in recent decades because its 3 Laboratoire de Biochimie, Hôpital Universitaire La Timone, survival is low mainly in the developing African Marseille, France. 4 Clinical Pharmacokinetics Laboratory, SMARTc unit, Inserm countries. The aim of this survey to provide an S 911 CRO2, La Timone University Hospital of accurate region-wide statistics of CNS tumors in Marseille, France. a North African country (Western Algeria) by [email protected] undertaking and epidemiological retrospective descriptive parameters such as: age, gender, symptoms, staging and histology. Our Abstract retrospective study was performed using a Cytidine deaminase (CDA), a liver enzyme database of 191 patients with CNS cancer who playing a crucial role in the metabolism of had been diagnosed at the neurosurgery analogues, is widely prescribed in adult and department of the University Hospital Center of pediatric oncology. The gene coding for CDA is Sidi bel Abbes, from 2010 to 2017. 191 patients characterized by its high genetic variability (50.26 % females and 49.47 % males were resulting in severe toxicities, or on the contrary a diagnosed with brain cancer). We reported a therapeutic escape. A stratified typing strategy of slight female predominance with the male to CDA status should reduce these risks and female ratio of 0.989, the mean age was about increase therapeutic efficiency. The present 43,10 years, ranging from 12 to 80 years old. The study aimed to validate analytically a visible most common medical histories recorded among spectrometric method allowing a rapid and low- our patients were: High blood pressure (13.6%), cost establishment of the patient's followed respectively by diabetes (10%). "CDA" functional status, by Concerning tumors’ sites, the most noticeable measuring serum residual activity. In the present ones were: supratentorial (71.2%), infratentorial study we performed a simple phenotypic test, (19.9%), medullary (7.3%) and cervical (1.6%). requiring 20 μl of serum and based on a The most predominant histopathological types visiblespectrophotometric measurement. The were: meningiomas (32.6%) and glioblastomas test aims was carried out to establish at low cost (21%), astrocytomas (9.4%). The most noted and quickly the patients’ CDA functional status, symptoms were: Intracranial pressure (62.8%), by measuring the serum residual activity. The epileptic seizures ( 23.6%), sensitivo-motor analytical validation concerned the measurement deficiency (31.4%). North African analysis of domain study, the reproducibility and the

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repeatability. A cross validation was carried out determining the influence of XRCC3 gene on serum specimens to evaluate the concordance Thr241Met of the XRCC3 gene on the level of the results between the laboratory of susceptibility to develop the CP. We have Biochemistry, CHU Annaba, Algeria and the undertaken a casecontrol study of this laboratory of transfer in oncology of the North polymorphism in a sample of the Algerian West University Hospital, Marseille, France. Our population. This case-control study involved 10 results showed that the method was linear in the subjects with CP and 20 unrelated normal range of 0.25 to 8 U. Moreover, the statistical subjects. The various genotypes of the processing of the data obtained showed good polymorphism were investigated using fidelity; satisfactory repeatability, homogeneous polymerase chain reaction - restriction fragment reproducibility between all the variances (CV length polymorphism (PCR-RFLP) technique. The <5%) and an acceptable Cochran test. Our comparison of allelic and genotypic frequencies findings allowed us to validate this method of between the two groups was established by measuring the CDA serum residual activity at the calculating the odds ratio (OR) with a 95% Annaba CHU biochemistry laboratory. The confidence interval. Statistical analysis showed phenotypic screening CDA and prior knowledge no statistical significant difference of distribution of the patient metaboliser status should of allelic and genotypic frequencies (OR = 0.36 contribute to adjust the dosage of nucleoside [0.12-1.1], P = 0.7) of this polymorphism analogs, in order to improve the balance explored between Cases and controls efficiency / toxicity. distribution. In conclusion, it would be very important to increase the number of cases Key words: Cystidine deaminase, gemcitabine, studied and to explore other polymorphisms cytarabine, toxicity, efficacy. located on the XRCC3 gene and also located on other genes involved in DNA repair, for the most precise genetic characterization of prostate cancer in the population of western Algeria. P037 Keywords: Prostate cancer; XRCC3; Thr241Met; The genetic association between rs861539 Algerian population. and prostate cancer in Algerian population: cases-controls study

Boughrara W.1,2,3*; Aberkane M 2,3,4; Moghtit F.Z 2,3,5. P038

Ecole préparatoire en science de la nature et de la vie d’Oran Study of genetic association between (EPSNVO) Laboratoire de génétique moléculaire et cellulaire / polymorphism Insertion / deletion of the Université des sciences et de la technologie d’Oran Mohamed angiotensin converting enzyme gene, the ACE Boudiaf (USTO-MB), BP 1505 EL-M'NAOUAR- 31000 ORAN- gene and the ABO gene in breast cancer in the Algérie. Service de cytogénétique et de biologie moléculaire, population of Tlemcen Etablissement hospitalo-universitaire d’Oran (EHUO). Département de pharmacie / Université d’Oran 1, Algérie. Chahinaz Benkheira*, Houssam Boulenouar , Asmaa Département SNV, institut des sciences, Centre universitaire Rahoui, Kaouel Meguenni d'Ain Témouchent "Belhadj Bouchaib". [email protected] CANCERLAB, départment of medicine ,university of Tlemcen [email protected]

Absract: Abstract In Algeria, breast cancer represents a real public In Algeria, prostate cancer (CP) is a real public health problem, which combines the involvement health problem. It combines the involvement of of genetic and environmental factors. Several genetic and environmental factors. Many genes genes are associated with the risk of occurrence are associated with the risk of this pathology, of this pathology, some of which code for some of which encode proteins involved in DNA proteins involved in different mechanisms of the repair mechanisms. Our study consists in human body. In this work, we carried out a case-

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control study in order to search for and highlight P039 possible associations between the ABO, ACE gene polymorphisms and the occurrence of breast Are there any other mutations of the RB1 cancer in the population of western Algeria. gene in patients with retinoblastoma in Specifically in the Tlemcen region, the study Algeria? sample included 26 breast cancer patients and 39 unrelated normal subjects diagnosed and Amina Mama Boubekeur1, Djabaria Naima removed from the gynecology department at Meroufel1*, Lotfi Louhibi1, Khadidja Mahmoudi2, Tlemcen University Hospital. After statistical Fatima Zohra Moghtit1, Meriem Aberkane3, Rym 1 1 analyzes, our results have shown that the Khadidja Abderrahmane , Meriem Abdi , Aness Kouar4, Faouzia Zemani-Fodil1, Nadhira Mehtar1 occurrence of breast cancer in our population is characterized by an early age of onset; the 35 to 1Laboratoire de Génétique Moléculaire et Cellulaire, 60 age group is the most affected segment of the Département de génétique moléculaire appliquée, Université population disease. Hormonal status is a major des Sciences et de la Technologie d’Oran USTO MB, BP 1505 risk factor for occurrence , statistical analyzes EL M’naouer 31000 Oran Algérie. 2 Service d’Ophtalmologie pédiatrique, Etablissement reveal that patients with a positive ER are four Hospitalier Spécialisé « Canastel » Oran, Algérie. times more likely to develop breast cancer with 3 Département de pharmacie, faculté de médecine, université one (OR = 4.58, P-value = 0.029). On the other Oran1, BP El M’naouer 31000 Oran Algérie. hand, blood group A is a major risk factor. Our 4 Service de toxicologie, EHU Oran Algérie. statistical results indicate that the risk of [email protected] occurrence of breast cancer is multiplied by four times in women of the blood group A with one Abstract (OR = 4.71, P-value = 0.009). To our knowledge, this study represents the first approach of the Retinoblastoma (RB) is a malignant tumor of the kind on an Algerian population, the encouraging retina of neuroepithelial origin. Its incidence is results obtained, suggest that it would be stable, described around 1 / 15,000 births in interesting to enlarge this study by increasing the Algeria. The retinoblastoma gene, RB1 gene is sample size, so as to be able to determine the size located on chromosome 13 at q14.2, has a major of the sample. social and environmental factors physiological function of controlling the cell on the one hand, and to explore other genetic cycle. It is a tumor suppressor gene. Inactivation polymorphisms located in other genes such as of both alleles of the RB1 gene during normal the BRCA1 and BRCA2 genes, on the other hand, retinal development initiates the formation of a in order to characterize in a more precise way retinoblastoma tumor. RB1 screening remains the genetic determinism of breast cancer in our difficult, most of the alterations being unique and population, with the aim of proposing a breast randomly distributed throughout the entire cancer prevention strategy better adapted to our coding sequence. In this report, we present the population. results of a constitutional and tumoral RB1 analysis in an Algerian population. The detection Keywords: ABO gene, ACE gene, breast cancer, of RB1 gene deletion or mutation was performed genetic study. by high performance liquid chromatography (HPLC) and sequence analyses in 21 patients. Germline abnormalities were found in 2/21 patients of sporadic unilateral retinoblastoma. The spectrum of germline and tumoral alterations included 3 nonsense mutations, 1

mutation affecting splice site, 1 deletion and 2 polymorphisms. These mutations have been reported in the Chinese population. In general, for the 21 patients with no family history of the disease we have identified mutations in germinal level in two of them showing that it is a transmissible form of retinoblastoma in these two cases known to be sporadic. A total of 2 mutations have not been previously reported.

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These results can be confirmed by increasing the chlorophenoxy)-2-hydroxypropyl moieties are number of patients studied, and by conducting underway. larger family studies. Keywords: minobenzimidazole; cytotoxicity; Keywords: Retinoblastoma; Rb gene; HPLC; PCR anticancer; synthesis sequencing.

P041 P040 Genetic polymorphisms implication of Synthesis and evaluation of 1H- estrogen metabolising enzymes in breast benzo[d]imidazol-1-yl-3-(4- cancer susceptibility: ongoing study on east chlorophenoxy)propan-2-ol derivatives as Algerian women cytotoxic agents Merabet Nabila1, Hambaba Leila2, Benseddik Nora Chouha1, 2, *, Hassan Hammoud1, Laurent Khedidja 3, Ibrir Messaouda4, Yakhlef Sihem 4 Desaubry1, Simone Brogi 3. 1Department Of Biology Of Organisms, Faculty Of Nature And 1 Laboratory of Therapeutic Innovation (UMR 7200), Faculty Life Sciences, Mostefa Benboulaid Batna 2 University,53 Route of Pharmacy, University of Strasbourg–CNRS, 67401, Illkirch, De Constantine,Fesdis, Batna 05078, Algeria. France. 2Laboratory Of Cellular Physiopathology And Bioactives 2 Laboratory of Synthesis of Molecules of Biological Interest, Molecules Biotechnology (LBMBPC), Faculty Of Nature And University of Constantine 1, 25000 Constantine, Algeria Life Sciences, Mostefa Benboulaid Batna 2 University. Batna 3 European Research Centre for Drug Discovery (Nat 05000, Algeria. SynDrugs), Department of Biotechnology, Chemistry and 3Department Of Pharmacy, Faculty Of Medicine, Mostefa Pharmacy (DBCF), University of Siena, Siena, Italy Benboulaid Batna 2 University, Route De Tazoult, Batna [email protected] 05000.Algeria 4Anatomo-Pathology Department, CHU Benflis Touhami, Allées Mohamed Boudiaf, Batna 05000. Algeria [email protected] Abstract

In the course of our research on the development of new anticancer agents we discovered that Abstract iminobenzimidazole 3a is cytotoxic in A375 Breast cancer is the most common fatal melanoma cells. Considering that 3a had been malignancy among women, in Algeria, it’s about described in a small number of patents and 11000 new cases identified per year. In breast articles, none of them related to cancer, and since cancer etiology, phase I and II detoxification of the anticancer potential of iminobenzimidazoles estrogen metabolites are described to play a role has been scarcely investigated, we explored the in carcinogenesis. Cytochrome P450 1A1 requirement of 3a for its cytotoxicity to (CYP1A1), Cytochrome P450 1B1(CYP1B1), determine whether it could provide the basis for catechol‑O‑methyltransferase (COMT) and the developement of new anticancer agents. glutathione S-transferase pi (GSTP1) enzymes, -5 Cytotoxicity at 10 in KB cells after 72 h are implicated in xenobiotics metabolising treatment of representative examples of 3a system, therefor, their expressions are influenced analogues expressed as % of cell death. The by genetic factors such as genetic research and development of novel type of drugs polymorphisms, and environmental factors such is of great importance to improve the treatment as exposure to polycyclic aromatic hydrocarbons of cancers. In this context, we describe herein the (PAHs) and xenobiotics. Since these enzymes are discovery and optimization of a first-in-class involved in carcinogenic metabolism; there are a series of cytotoxic agents. The SAR studies possible implication in breast cancer demonstrated the requirement for a hydrophobic susceptibility. To study the relationship between moiety. These results are highly encouraging, and CYP1A1, CYP1B1, COMT and GSTP1 SAR studies to examine structural variations of polymorphisms and breast cancer risk, five the iminobenzimidazole and 3-(4- polymorphisms CYP 1A1 * 2C (m2), CYP 1B1 A119S, CYP1B1 N453S, COMT V158M et GSTP

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I105V are chosen to be assessed using PRC-RFLP selected proteins and quantitative analysis of cell approach. Pathological RFLP (restriction cycle, and apoptosis phenotypes. On average, fragment length polymorphism) profiles were complex 2 was 78× more potent than complex 1, compared to healthy RFLP profiles. Preliminary 36× more active than cisplatin, and strongly results show that CYP1A1 M2 polymorphism is active (nanomolar) in patient-derived ovarian potentially associated with breast cancer cancer cell lines. RNA sequencing of A2780 susceptibility. Enlargement of sampling on east ovarian cells revealed upregulation of Algerian population are in progress and needed antioxidant responses (NRF2, AP-1) consistent for a better understanding of the role of these with observed induction of reactive oxygen polymorphisms in breast cancer occurrence. species. Protein microarrays, high content imaging and cell cycle analysis showed S/G2 Keywords: Breast cancer; estrogen metabolism; arrest, and late-stage DNA damage response PCR-RFLP; polymorphism. without p53 requirement. The triple-negative breast cancer cell line OCUB-M was highly sensitive to the complex 2 as were cell lines with KIT mutations. Complex 2 exhibits a markedly P042 different pattern of antiproliferative activity compared to the 253 drugs in the Sanger Cancer Cancer pharmacogenomics strategies: Genome database, but is most similar to osmium investigations of organo-iridium anticancer (II) arene complexes which share the same complexes reveal novel mechanism of action azopyridine ligand. Redox modulation and DNA damage can provide a multi-targeting strategy, Sihem Derbal1*,, Yahia Dellaoui2 allowing compounds such as complex 2 to Affiliations 1. Faculty of Medicine of Oran: B.P 1510 ،Oran overcome cellular resistance to platinum 1 31000 anticancer drugs, and also innovating molecules University hospital center of Oran : Boulevard Doctor can be discovered by in silico screening for these Benzerdjeb, Oran 31000, Algeria new targets. On the same way, new 2 Affiliations 2 . Faculty of Medicine of Oran: B.P 1510, Oran 31000 Pharmacogenomic approches can be created University hospital center of Oran: Boulevard Doctor such as the integrative one used to systematically Benzerdjeb, Oran identify potentially cooperative genes and Oran 31000, Algeria provide novel insights to combination therapies [email protected] in cancer medicine.

Keywords: Pharmaco-genomic; Organo-iridium; Abstract Anticancer; Mechanism of action. Intrinsic and acquired resistance to platinum drugs used in over 50% of cancer chemotherapies is a major clinical problem. Other precious metal complexes with distinct mechanisms of action might overcome this. Half- sandwich organometallic complexes containing arene or cyclopentadienyl ligands show promise. A potent antiproliferative activity for organo- iridium(III) complex discovered by phenotypic screening can provide valuable insight into their mechanism of action. This subject report the results of phenotypic screening and in-depth mechanism of action profiling of two organo- iridium (III) complexes [Ir phenylpyridine Cl] and [Ir N,N-dimethylphenylazopyridine Cl]PF6 of 916 cancer cell lines with known gene sequences from 28 tissue types. Time-dependent up- and down-regulation of genes has been studied by RNA sequencing, together with the detection of

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P043 the mtDNA of cortex, cerebellum and hippocampus of the HgCl2 exposed rat brain as Wormwood polyphenol extract mediated compared to control. The concentrations of 8- remediation of mitochondrial DNA (mtDNA) OHdG were >2 folds in hippocampus and >1.5 oxidation and neurotoxicity induced by folds in cortex when 5 mg / kg body weight per mercury week for 10 weeks of HgCl2 was administered. A significant decrease in the 8-OHdG formation in Nouria Hallal1,2*, Imène Benyettou1,3, Omar the mtDNA of cortex and cerebellum and no Kharoubi1. significant decrease in the mtDNA of hippocampus of the polyphenol extract+HgCl2 1Laboratory of Experimental Bio-toxicology, Bio-depollution and Phyto-remediation, Department of Biology, University of exposed rat brain as compared to HgCl2 exposed Oran 1 Ahmed Ben Bella, Oran, Algeria. rat brain group. Earlier studies have suggested 2Ahmed Ben Yahya El Wencharissi University that mercury is genotoxic, whereas ROS Center.Tissemsilt. generation is associated with the effect of specific 3Higher School of Biological Sciences of Oran (ESSBO) oxidative DNA damage. One reason is the ability [email protected] of mercury compounds to bind directly with tubulin sulfhydryl, which impairs spindle Abstract function and causes chromosome aberrations. Results reported in the recent literature, on the Free radicals (ROS) are considered important whole, lend support to the hypothesis that factors in cancer. Attacks on DNA by ROS, dietary polyphenols protect the body against the including the hydroxyl radical (OH), superoxide effects of reactive oxygen species on DNA anion (O2-), singlet oxygen, the ferryl or perferryl integrity. In conclusion, the present stage of ion, hydrogen peroxide, and peroxynitrate knowledge about the impact of heavy metals in (OONO), frequently cause oxidative DNA damage. biological systems indicates the enhanced Recent studies have shown that DNA damage and formation of free radicals and ROS or RNS or altered intracellular redox status, such as their intermediates causing neurotoxicity and imbalance of sulfhydryl and redox enzymes, other ailments and that could be efficiently occur after exposure to mercury. Occupational managed by using different preparations from a and environmental exposure to chemicals may number of traditional medicinal plants. Although increase the risk of unrepaired DNA lesions, coadministration of antioxidants (natural, herbal, which could become permanent mutations. or synthetic) or with other chelating agents has Polyphenols are a very broad group of chemicals, shown to improve removal of toxic metals from widely distributed in plant foods, and endowed the system as well as clinical recoveries in animal with antioxidant activity by virtue of their models, still the in-depth clinical studies with numerous phenol groups. They are widely preexisting or newer chelating agents are studied as putative cancer-protective agents, required to be done to reap real benefit with the potentially contributing to the cancer preventive least side effects. In case of humans, it is also properties of fruits and vegetables. In this study, important to find out the suitable dose and we evaluated the relationship between mercury duration of treatment to decide the optimal exposure and mitochondrial DNA (mtDNA) therapeutic index for any of the drugs to be given damage, and we evaluated the protective role of in isolation or in different combinations. polyphenol exctact isolated from Artemisia absinthium L plant in imparting protection Keywords: 8-hydroxy 2-deoxyguanosine (8- against mercury mediated oxidative OHdG); mtDNA oxidation; polyphenol extract; mitochondrial DNA damage of cortex, mercury. cerebellum, hippocampus and striatum. Intra- mitochondrial accumulation of 8-OHdG was measured as the indictor of mtDNA oxidative damage in Cortex, cerebellum, striatum and hippocampus. In the present study, we observed a significant increase in the 8-OHdG formation in

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Cancer Epigenetics and Nanotechnology in cancer treatment

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PO44 PO45 Relationship between chemotherapy Clinical Interests of the study of oxidative treatment on oxidative stress markers in stress in gastric cancer-A case control-study- Algerian colorectal cancer patients – A case control Study Naima Badid1*, Youcef Bendiaf1, Hafida Merzouk1, Amine Charef3, Djalloul Hamzaoui4

Naima Badid1*, Hafida Merzouk1, Abdelwaheb Dib2, 1-Physiopthology and Biochemical of Nutrition Laboratory 3 4 Amine Charef , Djalloul Hamzaoui (PPABIONUT), Department of Biology, Faculty of Faculty of Sciences of Nature and Life & Sciences of the Earth and the 1-Physiopthology and Biochemical of Nutrition Laboratory Universe, University of Tlemcen, 13000, Algeria. (PPABIONUT), Department of Biology, Faculty of Faculty of 3-Oncology Service- Hospital Popular Establishment (EPH) - Sciences of Nature and Life & Sciences of the Earth and the Maghnia – Algeria. Universe, University of Tlemcen, 13000, Algeria. 4- Surgery Clinic Avicene, Maghnia, Algeria. 2-Department of Biology, Faculty of Faculty of Sciences of [email protected] Nature and Life & Sciences of the Earth and the Universe, University of Tlemcen, Algeria. 3-Oncology Service- Hospital Popular Establishment (EPH) - Abstract Maghnia – Algeria. 4- Surgery Clinic Avicene, Maghnia, Algeria. Reactive oxygen species (ROS) are involved in [email protected] the pathophysiology of gastric cancer (GC). ROS produced by the immune and epithelial cells, Abstract damage the host cells and can result in DNA damage. The species Helicobacter pylori has Many chemotherapeutic agents’ treatments evolved to evoke this damaging response depend on free radicals profile to kill cancer cells. while blunting the host's efforts to kill the In this research, we focus on the assessment of bacteria. This long-lasting state with the oxidative stress in colorectal cancer (CRC) inflammation and oxidative stress can result and then evaluate the effect of Xelox plus in gastric carcinogenesis. Few studies have bevacizumab on the redox status. Forty one specifically focused on the interaction of redox newly diagnosed CRC patients and fifty healthy status with treatment. In this study, we aimed to subjects were included in this study. Blood evaluate the redox status in DC patients and samples were collected from control subjects and investigate the association between ROS, patients before and after treatment. To antioxidants and chemotherapy. The study was investigate the association between redox conducted at the popular Hospital “EPH” of balance and CRC treatment, levels of ROS and Maghnia where nine patients of GC and forty one antioxidants were analyzed by healthy controls where recruited. Blood samples spectrophotometry. Our findings revealed an were collected from control subjects and patients increase in MDA, O2-, CP, NO, and ONOO- before and after treatment. Levels of ROS and before treatment and a decrease of SOD, CAT and antioxidants were analyzed by GHS. After treatment, MDA, O2- and CP levels spectrophotometry. The obtained results reveal a decreased in CRC patients, the concentration of state of under nutrition observed based on BMI, NO and ONOO- increased significantly in an evident oxidative stress (OS) by the patients. The SOD, CAT activities and GHS levels overexpression of ROS such as MDA, NO , were significantly higher after treatment at the opposite of plasma vitamin C concentration in ONOO before treatment, with a drop in catalase CRC cases. Chemotherapeutic drugs control the activity with overexpression of SOD face of OS in evolution of tumor cells by interacting with ROS cancer patients. The scheduled cure in an adaptive redox status, hence the encompassed (a) Oxaliplatine + Xéloda + importance of their regulation. Further studies Epiribucine + Taxotère; (b) Cisplatine + are required to search for the most promising Epiribucine + 5-FU ; (c) Epiribucine + oxidative biomarkers to predict response to Oxaliplatine + 5-FU (d) Epiribucine + Xéloda + treatment. Cisplatine. The treatment used showed interesting interactions varying from a positive impact to a negative one on the individual redox Keywords: Colorectal cancer; Oxidative stress; equilibrium and depending on the composition of Treatment response; Xelox-bevacisumab. the drug regimen. Chemotherapy control the

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evolution of GC including ROS synergy in an improving cares for patients. In our study, adaptive redox status, hence the clinical interests colorectal cancer affects people over 50 years as of their modulation to predict response to well as younger (16%). Colorectal cancer was treatment. more frequently found with an aggressive stage (42% of colorectal tumors with stage IV). 72% of Key words: Gastric cancer; Adaptive oxidative patients develop liver metastasis. Finally, CD10 stress; Treatment response; chemotherapy. overexpression in colorectal tumors was associated with liver metastasis (21%) and the presence of lymph nodes (60%), suggesting a poor prognosis. Our results showed that CD10 is P046 overexpressed in some colorectal carcinomas. CD10 a prognostic and predictive potential Accurate CD10 assessment by marker of liver metastasis in colorectal cancer immunohistochemical analysis in colorectal carcinomas is necessary and crucial. In the Cherifi Yasmina*, Addou-Klouche Lynda, Beidaoui future, it will be necessary to evaluate the Soumia efficacy of administering a CD10 inhibitor for preventing liver metastases in colorectal cancer. Département de Biologie, Universite Djillali Liabess, Sidi Bel Abbes, Algerie [email protected] Key word: CD10; Colorectal cancer; Liver metastasis; Therapeutic Target.

Abstract Colorectal cancer is the third leading cause of P047 cancer-related death in both men and woman in the world. It poses a major public health problem Colorectal Cancer and Oxidative Stress in the and there is renewed interest in understanding West of Algeria the basic principles of the molecular biology of colorectal cancer. In this context, recently Ouldcadi H.1, Bensaada F.Z.1, Tahari Z.2, Ghalek M.1, assessment of membrane metallo-endopeptidase Elkebir F.Z.1,Sahraoui T.1

(MME/CD10) expression by 1 Laboratoire de Biologie de Développement et de la immunohistochemistry in colorectal cancer and Différenciation, Département de Biologie, Faculté des Sciences its relationship with cancer progression and de la Nature et de la Vie, Université Oran1 Ahmed BENBELLA, prognosis were investigated. This work consists Oran, Algérie. B.P 1524 ,El M'Naouer -31000 Oran,Algérie of studying the characteristics of epidemiological 2 Université des Sciences et de la Technologie d'Oran Mohamed-Boudiaf and clinical-pathological aspects of colorectal El Mnaouar, BP 1505, Bir El Djir 31000, Oran, Algérie. cancer during the period of time from January [email protected] 2008 to December 2013. More than that, we aim to evaluate CD10 by immunohistochemical Globally, The CRC occupies the third place in both analysis on colorectal tumours and the sexes combined; its incidence is 10% and 9.2% in relationship of its expression with regard to the men and women respectively. That incidence is clinic-pathological characteristics. A due to several factors which are endogenous retrospective study was conducted on 364 factors (age, inheritance and personal history) or patients in the northwest region of Algeria, exogenous one (smoking, alcohol, physical diagnosed with colorectal cancer distributed activity and diet). Our work is projected in this between Sidi Bel Abbes university hospital, and axis of research. We studied the oxidative stress Oran millitary regional university hospital. of CRC patients in the West of Algeria. The Furthermore, we performed an current study was realised on 33 patients immunohistochemical study to evaluate CD10 presenting a colorectal cancer recently diagnosed expression level; that is expressed in colorectal without metastasis (22men, 11women), aged tumors cells and liver metastasis. Overexpression 54±9 years and compared to 30 individuals of CD10 may be related to unfavorable prognosis volunteers (10 men, 20 women) as control aged of patients with colorectal carcinoma thus, may 48±7 years. In this context, we try to evaluate the constitute a futur therapeutic target for oxidative stress markers in a colorectal cancer

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population living in the West of Algeria. Then, we that are revolutionizing the field of research, measure the Malondialdehyde (MDA) plasma including the development of nanocomposites level by the thiobarbituric acid (TBA) reaction materials that combine the properties of according to Quintanilha et al., 1982 and polymeric matrices with those of inorganic carbonyls plasma level according to the method nanoparticles, in particular magnetic ones. In the of Levine and al, 1990. We also estimate the present work, the properties of magnetic Catalase (CAT EC 1.11.1.6) activity according to nanogels based on natural/modified anionic Góth, 1991and superoxide dismutase (SOD EC biopolymer and magnetic nanoparticles were 1.15.1.1) activity as described by Marklund & al., evaluated. The different magnetic nanogels 1974 as antioxidant enzymes and plasma level of prepared were mainly studied by Fourier thiols as non-enzymatic antioxidant by the Transform Infra-Red Spectroscopy (FTIR), method of Hu,1994. All methods are colorimetric Swelling Degree (SD) and Scanning Electron and the IBM SPSS statistics version 20.0 software Microscopy (SEM). The characterization results was used for statistical analysis. Results are of nanogels with and without crosslinking have presented as mean ± standard deviation. shown the obtaining of different networks of Independent sample t-test was used to compare hydrogels during the formation of nanoparticles the mean between groups. A value of p<0.05, in the different systems. SEM analysis showed a designed as (*) on graphs, was considered to be better morphology of the polymer crosslinking statistically significant. Our results showed an network. The incorporation of NPs into nanogels increase in plasma carbonyls and can not only significantly improve their efficiency malondialdehyde levels in CRC patients but also their physico-chemical stability allowing compared to volunteers accompanied with a their use in different fields. These results indicate significant decrease in SOD activity and thiols that the magnetic biomaterials obtained have levels in CRC patients compared to volunteers interesting characteristics that can be exploited whereas a non-significant variation was noted as nanomaterials for pharmaceutical between CAT activities of both populations. Our applications. results reveal an excessive production of free radicals not compensated by the intrinsic Keywords: Cross-linking; Hydrogels antioxidant activity. This unbalance between nanocomposites; Magnetic nanoparticles; SEM. pro-oxidant and antioxidant in our study population indicates the presence of an oxidative stress. P049 Key-words: Oxidative stress, colorectal cancer, Contribution at the research of the association free radicals, antioxidant between the MTHFR gene polymorphism 677C>T and bladder cancer occurrence in a sample of the population of Western Algeria P048 Zohra Touala-Chaila1*, Warda Shama Belhadri Physicochemical Characterization of Magnetic Smain1,2, Rym Khedidja Abderrahmane1 , Naima Nanogels Based Natural Polymers Meroufel 1, Benseddik Naima, Mostefa Jamel Yousfi3, Slimane Kerroumi3, Faouzia Zemani-Fodil1 Meriem Boudoukhani1,*,Nadji Moulai- 1 Mostefa1,2,Sonia Lefnaoui2, Manuel Banobre-Lopez3. Laboratoire de Génétique Moléculaire et Cellulaire, Université des Sciences et de la Technologie d’Oran Mohamed

BOUDIAF -USTOMB- . 1Material and Environmental Laboratory 2Université Aboubekr Belkaid, Telemcen. 2University of Medea, Ain D’heb, 26001 Medea, Algeria 3Affiliation Etablissement Hospitalier Universitaire d'Oran 3 INL, International Iberian Nanotechnology Laboratory, EHU1 Novembre 1954, service Chirurgie Urologique. Braga, Portugal [email protected] [email protected]

Abstract: Abstract The growing development of nanotechnologies Urinary bladder cancer is the second most has led to the emergence of innovative materials common genital urinary tumour that causes

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more than 16,000 deaths per year. Tobacco the function and ultimate efficiency of consumption and exposure to carcinogens, most nanomedicines remain unsatisfactory for clinical of which are aromatic amines, are considered to application, mainly because of our insufficient be the main environmental risk factors that have understanding of nanomaterial/nanomedicine- an impact on the genetic disorder. MTHFR is the biology (nano-bio) interactions. The metabolism gene that has been sufficiently nonequilibrated, complex, and heterogeneous studied by suggesting its involvement in the nature of the biological milieu inevitably occurrence of the diseases. Several publications influences the dynamic bioidentity of about the Ala677Val polymorphism of the nanoformulations at each site, that could have MTHFR gene have elucidated its involvement in biocompatible or bioadverse outcomes. In this the occurrence of bladder cancer. The working subject we will see the interaction of goal was to find an association between the SNP nanoparticles with proteins. The nanoparticle c.677C>T of the MTHFR gene and the occurrence surface can modify the structure and therefore of bladder cancer in a sample from the western the function of the adsorbed protein thus Algerian population. The genotyping of a sample affecting the overall bio-reactivity of the of the population from western Algeria nanoparticle; several studies show these consisting of 20 cases and 23 controls was biological events: Titanium dioxide (TiO2) carried out by the classical PCR-RFLP technique, nanoparticle was shown to cause conformational followed by statistical analyses. The results change and reduce polymerization of tubulin, suggest that there is no significant difference which is an essential cytoskeletal protein. Gold between allelic and genotypic frequencies in nanoparticles were shown to influence cases and controls (p>0.05), this seems excluding conformational changes in the structure of the null hypothesis which concerns the presence bovine serum albumin (BSA) in a dose- of an association between the polymorphism dependent manner, whereas no major c.677C>T of the MTHFR gene and the occurrence conformational change was recorded for BSA of bladder cancer in the population of Western when adsorbed to carbon C60 fullerene Algeria. From these results described above, it nanoparticle. It can be assumed that interaction can be concluded that there is no association of the nanoparticle with cellular structures is between the c.677C>T polymorphism of the indirect and occurs mostly via the nanoparticle- MTHFR gene and bladder cancer in the studied protein corona complex (NP-PC) and not the bare sample in the Western Algerian population. nanoparticle surface. The NP-PC can thus influence the uptake of the nanoparticle by the Keywords: Bladder cancer; MTHFR; cell. The uptake might be either inhibited due to Polymorphism; C677>T; West of Algeria. loss of protein structure of an adsorbed protein, or facilitated due to unfolding of the adsorbed protein to access receptors on the cell surface. Nanoparticle-induced protein conformational P050 changes may affect the downstream protein- protein interactions, cellular signalling and also Cancer nanomedicine challenges and DNA transcription, which is particularly opportunities: the nano-bio interactions of important for enzymes. Loss of enzyme activity nanomedicines can result due to the conformational changes in the active site, brought about by the nanoparticle Sihem Derbal, Yahia Dellaoui surface. Likewise, such conformational changes

2 Faculty of Medicine of Oran: B.P 1510, can also increase the accessibility of the enzyme University hospital center of Oran: Boulevard Doctor active site for its substrate. The nanoparticle Benzerdjeb, Oran Oran 31000, Algeria surface may induce abnormal unfolding of the [email protected] bound proteins to form novel conformational Abstract epitopes or may also induce unfolding of the native protein structure to expose hidden The intrinsic limits of conventional cancer epitopes. Such occult epitopes may affect the therapies prompted the development and functionality of the bound proteins for e.g. application of various nanotechnologies for more elicitation of an unwanted immune effective and safer cancer treatment, However, response. Nanoparticles can also induce

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conformational changes in proteins that can lead on cancer cells, and whose efficacy is linked to a to fibril formation, fibrillation of proteins is new mode of DNA inhibition, which plays a key associated with diseases such as Parkinson’s and role in the metabolism and cell survival. This Alzheimer’s. The Perturbation of the native molecule could stop the division of highly specific structure of the bound protein depends on the cancer cells. All the reagents in this work were of surface of the interacting nanoparticle and analytical grade and used as received without together these two factors direct the bio- further purification. NaOH and H2SO4 (98%) reactivity of the nanoparticle. À comprehensive were used as initiator from sigma Aldrich understanding of the underlying mechanisms (French). The Maghnite-Na + (MMT-Na +) used in of nano-bio interactions is paramount for the this work came from a quarry located in Maghnia intelligent design of safe and effective (North West of Algeria) and was supplied by the nanomedicines. company "ENOF" (Algerian manufacture specialized in the production of non ferrous and Keywords: cancer therapy; nano- useful substances). The chemical composition bio interactions; nanomedicine; nanoparticles. and structure of MMT-Na + have been shown in (Table 1 and Figure 1). The cation exchange capacity (CEC) and clay surface were respectively 84 mEq (100 g-1) dried clay and 786 m2.g-1, and P051 its X-ray diffraction analysis ( XRD) shows that the spacing between MMT-Na + layers is 1.29 nm. Anti timoral nano molecules called bats: New The different molecules resulting from these process for synthesis namely caesarean organic syntheses and which summarize our reaction under effect of maghnite (Algerian research products have architecture or a MMT) structure like the frog or fox. All the chemical reactions have been catalyzed by a Fatima Zohra Zeggai *1,2 Abdelkader Rahmouni1 and heterogeneous and ecological support called 1 Mohammed Belbachir maghnite and which has a great catalytic activity

1Laboratory of Polymer Chemistry, Department of Chemistry, especially for the vinyl and heterocyclic Faculty of Science, University of Oran 1 Ahmed Benbella. BP N compounds and very remarks in several ° 1524 El M’nouar, 31000 Oran, Algeria researches. Our method of synthesis must 2Center of scientific and technical research in physicochemical include points of view on health and the analyzes (CRAPC), Tipaza, Algeria. [email protected] environment that is why we used a catalytic support, heterogeneous and ecological it is the maghnite. chemical results have been Abstract summarized by above figures above and biological tests reported positive test such Cancer is a disease caused by the transformation solubility and toxicity and good reaction with of cells that become abnormal and proliferate DNA support such as stopping cell divisions and excessively. These disordered cells eventually rearrangement of DNA genes of a cancer cell. We form a mass called malignant tumor. Cancer cells have identified a new class of molecules called tend to invade nearby tissues and become 'bat' that act very specifically on cancer cells, and detached from the tumor. They then migrate whose efficacy is linked to a new mode of DNA through the blood vessels and lymphatics to form inhibition, which plays a key role in the another tumor (metastasis).Although there are metabolism and cell survival. This molecule many different types of cancer, they all originate could stop the division of highly specific cancer as a result of abnormal and uncontrolled cell cells. The structures of these compounds were development. Phenolic compounds can be confirmed by SEM, FT-IR and NMR spectroscopy. considered as important parameters of A synthesized materials having good physical organoleptic (color, taste, and aroma). The use of chemical properties such as water solubility at phenolic compounds as natural antioxidants in room temperature and good biological food is in progress in relation to their role in properties such as the low degree of toxicity prevention of some cancers and cardiovascular which is tested on a bacterium and a rat. Results diseases. We have identified a new class of of our research are very interesting for stopping molecules called 'bats' that act very specifically

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cell divisions that is to say the rearrangement of This toxicity was related to the geometry and DNA genes of a cancer cell. surface characteristics of the nanomaterials. Furthermore, ROS generation was found to play a Keywords: Maghnite, heterocyclic, design, key role in driving the induction of genotoxicity. nanomolecules, orcenol, anti cancer, bat. Other research groups have examined the toxicity of single walled carbon nanotubes (SWCNTs) on human keratinocyte cells and signaling mechanism in keratinocytes upon P052 exposure to SWCNTs. They have concluded that exposure to SWCNTs is associated with increased Toxicity of carbon nanotube used in cancer oxidative stress and inhibition of cells therapy proliferation. The toxicity and efficacy of (CNTs) still need to be investigated further to pave a way Bensaid Nour El Houda1,2*, Bendjamaa Atika1,2,3, for biomedical applications and a better Rezk-kallah Haciba1,2,3 understanding of their potential applications to 1 Toxicology Pharmacology Service, University Hospital Center humans. of Oran, Algeria. 2 Pharmacie Department, Faculty of Medicine, University of Keywords: nanoparticals; carbon nanotube; Oran1, Algeria. 3Environmental Health Research Laboratory, University of cancer; nanotoxicology. Oran 1, Algeria. [email protected] Abstract P053 Cancer remains one of the greatest challenges The use of Gold nanoparticles for cancer faced across the world today. Nanomaterials radiotherapy have been shown promising for cancer therapy. Carbon nanotubes (CNTs) are suitable in this Nabil Ounoughi 1, Abdelmalek Boukhellout 1, Yamina regards, such that, they possess a high Dribi 1. photothermal cell destruction and optical absorbance efficiencies. When these CNTs were Laboratory of Radiation Physics and Applications Mohamed first introduced in complex biological systems, a Seddik Ben Yahia University, Jijel, ALGERIA [email protected] new research direction evolved: nanotoxicology. The present study aims at reviewing the toxicity Abstract of CNTs. This is a descriptive study on the The Global Cancer Observatory (GCO) announced toxicity of (CNTs) used in cancer therapy. This that in 2018 over 18 million of cancer case is study is based on the synthesis of a series of diagnosed and over 9 million case of death. experimental studies that have been the subject Radiotherapy is a key treatment and is currently of several publications. CNTs have attracted great used in 50% of cancer patients. The main goal of interest in biomedical applications such as radiotherapy is to maximize the dose of radiation advanced imaging; tissue regeneration, drug or in the tumor and minimize it in surrounding gene delivery, and cancer therapies. There is no normal tissues. An approach to optimize consensus on CNTs toxicity to humans, however, radiotherapy is to introduce nanoparticles with a number of studies have shown significant high Z element in tumor before irradiation. The toxicity in both cell cultures and in vivo animal aim of this work is to investigate the physical and model. One of many observed toxic responses is biological aspect of the use of gold nanoparticles genotoxicity, For example, it has been shown that (GNPs) in radiotherapy with Low Energy X-rays. CNTs can penetrate and accumulate in mouse PENELOPE is a Monte Carlo (MC) simulation embryonic stem cells inducing a 2-fold increase code has been developed to describe radiation in DNA damage through the generation of interaction with biologically materials. The code reactive oxygen species (ROS). In addition it has is used for the simulation a phantom containing been demonstrated that CNTs have the potential tumor and gold nanoparticles with different to induce the release of the TNF-α pro- concentrations irradiate with different low inflammatory mediator, which could result in the energy X-rays at different depth of tumor. The generation of ROS in monocytic cells in vitro. dose profiles at normal tissue and tumor were

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calculated to show the dose enhancement by 1() nanoparticle. Results have shown that the dose susceptibility 1 and dynamic magnetic enhancement increase linearly with GNPs hysteresis (DMH) of single domain ferromagnetic concentration and it has highest values at photon nanoparticles induced by an external ac field of energy between 50 keV and 60 keV. The arbitrary strength and orientation are treated via secondary electron produced makes it possible to Brown’s model of coherent rotation of the increase the radiolysis of water and the damage magnetization we demonstrated that the DMH of biological macromolecules such as DNA. Gold strongly depend on the DC magnetic field h0, and nanoparticles has a high degree of the antiferromagnetic parameter . biocompatibility, it can be used to optimize radiotherapy at low energy X-ray. It is proposed Keywords: Magnetic Hyperthemia, Specific to be used in Stereotactic Synchrotron Absoption Rate, Brown’ Model, Fooker Planck Radiotherapy (SSRT). In the future; GNPs will equation certainly play an important role in the treatment of tumors. P055 Keywords: Radiotherapy, Gold nanoparticles, Radiation dose enhancer, Monte Carlo Simulation 5-Fluorouracil loaded- Magnetic nanoparticles for Targeted Cancer Therapy

1,2 2 P054 Kheira Zanoune Dellali , Corina-Lenuța Savin , Mohammed Dellali1,2, Abdallah Ouagued1, Marcel Popa2,3 SuperAntiferromagnetic Nanoparticules- based Hyperthemia for cancers Treatment 1 Hassiba Benbouali University of Chlef, Algeria, 2”Gheorghe Asachi” Technical University of Iasi, Romania, Adda Missoum1 , Bachir Ouari2*, Fethi Benkhenafou2, 3Academy of Romanian Scientists, Bucuresti, Romania Smain Balaska3, Yuri Kalmykov4 [email protected]

1University Centre Tissemsilt · Faculty of Science and Technology Abstract 2 Abou Bekr Belkaid University, Physics department, BP 119 Cancer remains one of the world’s most Chetouane, Tlemcen, Algeria 3 Oran University, Ahmed Ben Bella Physics department, devastating diseases, and the number of new Oran, Algeria cases increases each year. Conventional delivery 4 Univ. Perpignan Via Domitia, Laboratoire de of chemotherapeutic agents led to the fast Mathématiques et Physique, EA 4217, F-66860, Perpignan, development of cellular resistance causing toxic France. side effects (cytotoxicity, neurotoxicity, [email protected] nephrotoxicity) on the entire body which limits the drug doses that can be administrated. Drug Abstract delivery systems can play a key role in the fight Magnetic hyperthermia is the process by which against cancers by delivering locally, to the cycling magnetic nanoparticles in a Alternatif tumor site, the anticancer drugs. Hybrid magnetic field leads to heat dissipation. It is an nanoparticle consisting of a polymeric matrix in ingenious method for the treatment of cancer which is dispersed a magnetic nanoparticles and reduction in size of non-malignant tumours. material (magnetite (Fe3O4), maghemite(γ- This Processus generates no side effects unlike Fe2O3), hematite (α-Fe2O3), etc.), are lately more classical therapies such as chemotherapy. studied for use as target delivery systems of Understanding the mechanisms of the magnetic antitumor drugs. Selection of the matrix-forming Hyperthermia is critical so as to produce polymer is based on the need for it to be particles with optimised properties for specific biocompatible, biodegradable, mucoadhesive, applications at nominal dose. All these possessing reactive functional groups in order to mechanisms are highly particle size dependent. achieve the cross-linking process under mild Several heating mechanisms are possible, reaction conditions. The present work proposes associated with susceptibility loss, hysteresis a method for obtaining of magnetic nanoparticles loops and viscous heating. The Magnetic based-chitosan, which fulfills the above-

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mentioned conditions being one of the most used P056 natural polymers for drug delivery. The magnetic nanoparticles have prepared by double Nanotechnology in oncology: Therapeutic crosslinking in reverse emulsion method, a aspects, toxicological risks and perspectives slightly acidic solution of chitosan in which are dispersed magnetite nanoparticles are emulsified Nada Ben Yakoub, 1,2,*, Ahmed BETTAYEB,3 Haciba in an organic solvent (toluene), using sodium REZK-KALLAH 4,5,6 tripolyphosphate (ionic crosslinking) and 1Pharmacy department, Faculty of medicine, University of glutaraldehyde (covalent crosslinking). The Oran, Algeria. process has been optimized through the 2Faculty of sciences and technologies, University of Lorraine, modification of certain reaction parameters France. (polymer ratio, stirring rate, crosslinker amount, 3Pharmacy department, Faculty of medicine, University of Oran, Algeria. etc.), in order to obtain nanoparticles with low 4Toxicology hospital service, University hospital center of dimensional polydispersity. The particles with Oran,Algeria . optimal characteristics (in terms of morphology, 5Research laboratory LRSE,Oran,Algeria. water swelling degree) have been analyzed from 6Pharmacy department, Faculty of medicine, University of the point of view of the capacity of Oran, Algeria [email protected] inclusion/release of specific drugs (5-FU). This study highlights the way of obtaining Abstract micro/nanoparticles based on natural chitosan chemically grafted with synthetic In the last decades, a lot of efforts have been poly(ethylene glycol) methacrylate or provided and a large number of scientists have poly(ethylene glycol) methyl ether acrylate been involved in cancer research. Therefore, through Michael addition. This study highlights several drugs have emerged for different cancers the way of obtaining magnetic nanoparticles treatments, notably for breast cancer. based on natural chitosan with low molecular Nevertheless, even if the efficacy of these drugs is weight. Magnetic nanoparticles obtained present undeniable, their lack of safety represents a a spherical shape and a relative low dimensional major drawback. It is in this perspective that a polydispersity. Nanoparticles obtained showed a new generation is born : nanomedicines. This relative high swelling degree (300÷600 %) which work aims to evaluate and quantify the global explained the high drug loading efficiency. The in effect of those promising drugs, in terms of safety vitro release results are encouraging, showing and efficacy, in the field of breast cancer that the amount of 5-FU released in 24 hours is treatment. A good search strategy of two major closed to the maximum dose usually electronic databases has been conducted before administered in patients with cancer. Double performing a meta-analysis, including strictly crosslinking in reverse emulsion is a method of randomized controlled clinical trials comparing obtaining hybrid nanoparticles with magnetic nanomedicines with conventional treatments for proprieties, capable of including, transporting different populations. The primary outcomes are and releasing antitumor drugs to the target, the most reccuring adverse effets, survival being constituting themselves as a potential solution a secondary outcome. Results were expressed in for cancer therapy. odds ratios and standardized mean differences respectively. 34 studies were included in this Keywords: Cancer; Hybrid nanoparticle; meta-analysis, 3 of which were unpublished. Magnetic nanoparticles; 5-Fluorouracil. Given the statistical heterogeneity of the results, a subgroup analysis was performed. We found that nanomedicines are significantly less cardiotoxic than conventional treatments (OR 0.30, 95% CI, 0.18-0.51), and they significantly decrease the rate of neutropenia in adjuvant

therapy (OR 0.35, 95% CI, 0.24-0.52). On the other hand, they seem to accentuate some adverse effects: Hand-foot syndrome, Stomatitis / mucositis. No big differences were found for leukopenia and alopecia, neither for nausea /

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vomiting, fatigue and neuropathy of grade 3 and concern inorganic nanomaterials which call into above. Efficacy results seem to be in favor of question the relatioship of mankind to nanomedicines. According to our results, technologies and thus, many ethical and moral nanomedicines demonstrated favorable toxicity interrogations remain stirred up. profiles with less cardiotoxocity and myelosuppression in addition to an efficacy equal Keywords: Breast cancer; Meta-analysis; to or better than conventional treatments wich Nanomedicine; Nanotechnology. make them the drugs of choice for breast cancer treatment especially for elderly and cardiac populations. Other more interesting perspectives

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Novel approaches In cancer therapies

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P056 P057

Chemopreventive effect of luteolin on 1, 2- Phytochemical screening and evaluation of dimethylhydrazine induced experimental the antioxidant activity of the methanolic colon carcinogenesis extract of Rubia Tinctorum

Soumaya Louahem, Kheireddine Ouali, Fouzia Trea. Houari Fatima Zohra, Hariri Ahmed.

Faculty of Sciences, Laboratory of Environmental Bio Surveillance, BP 12 El Hadjar, University of Badji Mokhtar LBGMSS, Universite Mustapha Stambouli, Mascara, Algeria Annaba 23000 Algeria. [email protected] [email protected] Abstract Oxidative stress is recognized as a key player in the development and complications of many Colorectal cancer (CRC) is the third chronic diseases. For the sake of prevention, it is mostcommonly diagnosed cancer in males and therefore necessary to have powerful tools for the second in females. Globally there were 1.2 correctly assessing the oxidative stress status, in million cases of CRC yearly resulting in about order to make the necessary corrections to 608,700 deaths from CRC accounting for 8% of optimize the antioxidant defenses and to reduce all cancer deaths. Colorectal cancer (CRC) is a the oxidative damage induced by the activated leading cause for cancer-related death and its oxygen species (EOA) at the level of DNA, prevention is of great importance throughout the proteins and lipids. In this perspective, we have world. Chemoprevention offers a novel approach considered to characterize the phytoconstituants to control the incidence of colon cancer. The of this plant and to determine the biological present study was performed to evaluate the activity (antioxidant). In order to study the anti- efficacy of lutéolin supplementation on colonic radical activity of the various extracts of the aberrant crypt foci (ACF), lipid peroxidation, and leaves of Rubia Tinctorum, we used the method antioxidant defense system in 1,2- based on the DPPH. The reduction of the free dimethylhydrazine (DMH)-induced colon radical DPPH by an antioxidant can be followed carcinogenesis in male Wistar rats. Twenty male by UV-Visible spectrometry, by measuring the Wistar rats were randomly divided into 4 groups decrease of the absorbance at 517 nm caused by (5 rats/group). The control group was fed a the antioxidants. The results suggest that the basal diet. DMH-treated group (DMH) was fed a methanolic extract has good antioxidant activity basal diet and received 1, 2-dimethylhydrazine with (IC50 = 0.538 mg / mL). The results of the (DMH) intraperitoneal injections of DMH for two phytochemical screening are positive for the weeks at a dose of 30 mg/kg. The luteolin majority of phytoconstituents (alkaloid, phenol, treatment group (Lu) received oral flavonoid, and terpene). The results of this study supplementation of luteolin (30mg/kg) for 1 revealed that this plant has antioxidant agents month, and the fourth combined group received and this can either suggest the use of this plant in DMH and luteolin (DMH +Lu). The colon tissues the pharmaceutical industry as well as in the were examined microscopically for pathological food industry. changes and aberrant crypt foci (ACF) development, oxidant status, enzyme Keywords: antioxidant activity, DPPH, Rubia antioxidants system (GSH, GST). Our results tinctorum showed that DMH induced ACF development and oxidative stress (glutathione depletion) in rat colonic cells. The concomitant treatment of DMH with luteolin, significantly ameliorated the cytotoxic effects of DMH. The results of this study provide in vivo evidence that luteolin reduced the DMH-induced colon cancer in rats, through their potent anti-oxidant activities.

Keywords: 1,2-dimethylhydrazine(DMH), colon cancer, chemoprevention, oxidative stress.

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P058 PO59

Protective effect of extracts of propolis on Effects of polyphenols on lymphocyte liver injury induced by cisplatin used in proliferation and telomere length cancer therapyin mice Amel Zoubeyda Saidi Merzouk, Amel Medjdoub, Safia Ali Haimoud1*, Leila Ali Haimoud2, Rachida Hafida Merzouk, Bouchra Loukidi, Sid Ahmed Allem1, Kheira Zerouki3, Khawla Boutara3, Merzouk. Fatiha Belhachemi3. 1 Laboratoire PPABIONUT, Faculté SNVTU, Université de 1 Laboratory of Natural Local Bio-Resources, Département de Tlemcen, Algérie 1. Nutrition et Sciences des Aliments, Faculté des Sciences de la [email protected] nature et de la vie, Université de Chlef, Algérie. Abstract 2 Centre Hospitalo-universitaire d’Oran (CHUO), Algeria 3 Département de Nutrition et Sciences des Aliments, Faculté Despite the production of free radicals in the des Sciences de la nature et de la vie, Université de Chlef, body is inevitable, they become toxic responsible Algérie. [email protected] for significant cell damage and are implicated in the etiology of several human diseases. Considerable research has been aimed at Abstract reducing the effects of oxidative stress by use of Cisplatin (CP) is one of themost effective free radical scavengers derived from nutritional anticancer drugs in the treatment of solid sources, such as vitamins and polyphenols. In tumors. However, hepatotoxicity is the major addition, increasing oxidative stress affects adverse effectrepresenting a dose-limiting factor telomeres which are specialized DNA-protein in cisplatin therapy. Therefore, the objective of structures found at the ends of chromosomes and this study was to investigate the effects of are markers of genomic integrity and age-related extracts of propolis against cisplatininduced liver metabolic dysfunction. Antioxidant injury in mice. The micewere intragastrically supplementation could improve age–related administrated with different extract of propolis abnormalities by protecting telomere length. (250 mg/kg/day) for 3 days, and the liver injury Polyphenols have been shown to protect against models was established by single intraperitoneal oxidative stress and reduce risks of metabolic injection of CP (10 mg/kg). Liver samples were disorders. However, their effects on telomere taken for determination of histopathological length remain poorly understood. The aim of this changes. The oral administration of the extracts study was to determine in vitro effects of two reduced histological changes (inflammation) polyphenols (chlorogenic acid, curcumin) on cell induced by the injection of cisplatin in the liver. proliferation and cell ATP levels in lymphocytes This effect may be due to the antioxidant activity exposed to oxidative stress. Telomere length was of the phenolic compounds present in the also determined. extracts associated with less increase in hepatic Peripheral blood lymphocytes were isolated iNOS protein content. The present study showed using a density gradient of Histopaque. They that apicultural productscontain components were in vitro cultured and stimulated by Con A in that can be used in the food and pharmaceutical the presence or absence of free radical generator industry and are effective hepatoprotective (H2O2/FeSO4) and of polyphenols (final agents, with potential to ameliorate cisplatin- concentration 10 μM), for 48 hours in a CO2 induced hepatotoxicity. incubator. Cell proliferation was determined by MTT assay. Cell ATP content was assayed by Keywords: Cisplatin; Hepatotoxicity; Propolis; appropriate ELISA kit. DNA was extracted from Phenolic compounds. lymphocytes and Telomere length was quantified using quantitative PCR. The present study demonstrated that proliferation rate of T-lymphocytes was decreased with free radical generator resulting from intracellular oxidative stress and ATP depletion. Indeed, telomere length was reduced under oxidative stress. Free radicals reduced

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lymphocyte proliferation by causing cell (Met.E) of Zizyphus lotus known for its anticancer membrane, nucleic acid and protein damages effects, harvested from western Algeria, El- that can lead to energy depletion and cell Mamounia region, Mascara. The Met.E was apoptosis and death. Chlorogenic acid and extracted by cold maceration in methanol 80% curcumin improved significantly lymphocyte and with agitation 24h. The toxicological study proliferation and increased ATP levels. This was performed in Wistar rats by oral improvement was accompanied by an increase in administration of a non-lethal dose: 5000mg/kg telomere length. These beneficial effects were body weight (b.w). The detection of any toxicity more pronounced with curcumin. Previous was carried out by determining the behavior and results suggested that polyphenols may play clinical signs, weight evolution of the treated rats important roles in the protection against and by the determination of the presence of oxidative- induced cell injury by reversing the mortality. Thus, biochemical parameters have decrease in cell proliferation and ATP levels been investigated for the detection of any induced by free radical generators. In conclusion, discrete toxicity. Methanolic extract of Z. lotus we proved the in vitro positive effects of prepared during this study presented a highest polyphenols on lymphocyte function; these yield (14, 02%) than obtained by. Administration polyphenols might contribute to immune of Met.E by gastric gavage showed no evidence of improvement during oxidative stress. They toxicity in wistar rats, no change in behavior was protect telomeres and could be promising agents observed, a normal weight evolution, no for the prevention of age-related immune mortality was recorded and the biochemical alterations. parameters were consistent with the control group. The dose of Met.E used during this study Keywords: ATP; Chlorogenic acid; Curcumin; showed no secondary effect on wistar rats. This Lymphocytes; Telomeres. result indicate that the genus Zizyphus can be exploited in the medical field for treatment of cancer pathologies with no risk to human health. P060 Keywords: Acute toxicity; Methanolic extract; Acute toxicity of the methanolic extract of a Zizyphus lotus; Western Algeria. medicinal plant from western Algeria:

Zizyphus lotus in wistar rats P061 Nour Elhouda Bekkar1*, Boumediene Meddah1, Yavus S. Cakmak2 Medicinal plants from Algeria: A complementary and alternative therapy for Laboratory of Bioconversion, Microbiological Engineering and Sanitary Security, University of Mustapha Stambouli, cancer Faculty of Nature and Life Sciences, Department of Biology, El-Mamounia street 29000, Mascara, Algeria. Houari Benamar1,2*, Malika Bennaceur2,3, Abderrazak Department of Biotechnology and Molecular Biology, Faculty Marouf4. of Science and Letters, Aksaray University, Aksaray, Turkey. [email protected] Department of Biology, University of Mostaganem Abdelhamid Ibn Badis, PO Box 188, 27000 Mostaganem, Algeria. Abstract Department of Biology, University of Oran1, PO Box 1524, El M'Naouer, 31000 Oran, Algeria. Scientists interested by the traditional medicine Laboratory of Research in Arid Areas, PO Box 32, El Alia, Bab used a various genus of medicinal plants to Ezzouar, 16111 Algiers, Algeria. Laboratory of Sustainable Management of Biological isolate and identified a numerous bioactive Resources from Arid and Semi-arid Regions, Department of compounds (polyphenols) for the treatment of Natural Sciences and Life, University Center of Naama Salhi human diseases, such as cancer pathology. A Ahmed, P.O. Box 66, 45000 Naama, Algeria. various recently studies of the antitumor activity [email protected] of plant extracts were published and its used Abstract must be after studying their toxicity to the organism. The main objective of this study was to Cancer is a major public health problem evaluate the toxicity of the methanolic extratc worldwide and it is a complex disease involving

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many biological factors, processes and systems, P062 which may severely limit the actual efficacy of anticancer approaches. Even though numerous In vitro Cytotoxicity and Ex ovo Anti- anticancer drugs are available now, yet their Angiogenic Activity of Lemongrass effectiveness and usefulness are highly debatable (Cymbopogan citratus) Essential Oil against particularly because of their adverse effects. As Breast Carcinoma Cell Line herbal cures are usually linked with a good safety profiles therefore, they have the possible Mohamed Nadjib Boukhatem 123*, Noureldien H.E. potential to deliver effective substitutions to Darwish 1, Thangirala Sudha 1, Hanady Nada 1, Mehdi presently available synthetic anticancer agents. Rajabi1, Shaker A. Mousa 1 The present study aims to describe the plants 1 The Pharmaceutical Research Institute, Albany College of used in Algeria to treat cancer, as reported in the Pharmacy and Health Sciences, 1 Discovery Drive, Rensselaer, literature. The ethnopharmacological data were New York, USA. found through a research conducted on several 2 Département de Biologie et Physiologie Cellulaire, Faculté works on the medicinal plants of Algeria. The des Sciences de la Nature et de la Vie, Université – Saad used scientific nomenclature is according to The Dahlab – Blida 1, Blida, Algeria. 3 Laboratoire Ethnobotanique et Substances Naturelles, Ecole Plant List. In the current study, we have Normale Supérieure, Kouba, Alger, Algeria. presented the plants used (159 species) for the [email protected] therapy of different kinds of cancer. Additionally, we have provided a list of medicinal plants showing local or vernacular name, the origin, Abstract part used, method of preparation and The search for new drugs that display activity administration, other ingredients used as a against several types of cancer has become one of mixture, therapy type and kind of cancer treated. the most interesting subjects in the field The most plant genera used in traditional of natural products research. The aims of this medicine for the treatment of cancer are study were to investigate the in vitro irritation Aristolochia L. (Aristolochiaceae), Artemisia L. index and antiproliferative activity and ex ovo (Asteraceae) and Urtica L. (Urticaceae). Breast anti-angiogenic effects of Cymbopogon citratus cancer is the most commonly treated cancer by essential oil (CCEO) and its major compound herbs. The plants used are the most common and (citral). CCEO was isolated using a steam most easily found in Algeria. The reported plants distillation method and tested for cytotoxicity with anticancer properties, seem to be activity and anti-angiogenic activity using a interesting and to which modern pharmacology breast carcinoma cell line (MDA-MB 231). can appeal. Additional studies are necessary to Cytotoxicity activity was determined by using the completely identify the phytochemicals MTT assay and the anti-angiogenic activity was responsible for the reported anticancer effect, determined by using a chorioallantoic membrane and further research are needed therefore to of fertilized hen's egg (HET-CAM assay) and determine the exact mechanisms involved in this compared to a standard angiogenic substance. disease. The chemical composition of CCEO was determined with gas chromatography and Keywords: Cancer; Ethnopharmacology; revealed that citral was the major compound Medicinal plants; Phytotherapy. (60.3%), followed by menthyl acetate (9.5%) and menthone (5.5%). The irritation potential test in

the Red Blood Cell (RBC) system cellular model allowed the quantification of adverse effects of CCEO on the plasma membrane of RBC and the consequent release of hemoglobin (hemolysis), which enables the determination of the irritation degree. CCEO manifested high hemolytic activity (H50 = 0.4614%) and a great attention will take with greatest concentrations. The ex ovo test using the HET-CAM assay was used for the

determination of the anti-angiogenic effect of CCEO and revealed that oil-treated CAMs 78 Oran 05-07 December

branched into more multi-stage capillaries and recruited to the Hematology Department of the more abundant neo-vasculatures. Our results in Tlemcen University Hospital Center. All patients the HET-CAM assay suggested a moderate anti- underwent first-line chemotherapy (CHOP: angiogenic effect (angiogenesis index branch cyclophosphamide, adriamycin, oncovin, points = 60±10) of the CCEO at the tested prednisone), with a number of courses ranging concentrations (2µL/pellet) in comparison with from 1 to 8. Dietary assessment revealed a daily basic Fibroblast Growth Factor (140±9) at the diet reduced in calories, protein, carbohydrates, dose of 1 µg/mL. Breast cancer cells were treated fiber, saturated and polyunsaturated fatty acids with CCEO concentrations ranging from 4 to and vitamins E and C and observed at a low level 0.03% for 24 and 48h. CCEO has a potential of plasma ORAC. The study of demographic cytotoxic effect on MDA-MB 231 with IC50 value characteristics of practice has revealed the of 0.063%. Data obtained in these experiments presence of risk factors, namely smoking. The suggest that further investigations are warrented analysis of the biological assessment has using CCEO on various cancer cell lines due to its objectified a disturbance of lipid and protein anti-angiogenic effects observed in this work. profiles, including increased serum triglycerides, LDL-TG, the VLDLc and decreased levels of HDLc Keywords: Angiogenesis; Anticancer effect; and α-LP and a significant increase of albumin Breast Cancer; Cymbopogon citratus Essential Oil. and alpha-1 globulin in line with high CRP found in our assays. However the rate of gamma globulin was significantly lower in patients. Daily food intake can be reduced to cause lowering of P063 plasma levels of ORAC and observed the altered metabolism of lipids and proteins observed may Oxidative Stress in Patients with Non- be related to the poor prognosis of patients and Hodgkinal Lymphoma often poses the problem therapeutic care during chemotherapy. Allioua Meryema, Bouali Waffab ,Aribi Mourad

a Institut des sciences et techniques Appliquées ISTA, Keywords: Non-Hodgkins Lymphoma; serum b LASNABIO laboratoire des sciences naturels et Bioactives, protein; oxidative stress; inflammation. Faculté des sciences de la terre et de l’Univers, Université Abou-Bekr Belkaïd, P.O. Box 119, Tlemcen 13000, Algérie. mariaall260 @yahoo.fr P064

Abstract The oyster mushroom, an edible mushroom Non-Hodgkin's lymphoma is a heterogeneous rich in antioxidants, to prevent cancer group of lymphatic system cancers that have different biological characteristics based on a combination of morphological, ALIM Y1*, DIB S, ,FORTAS Z immunophenotypic, genetic and clinical criteria. Laboratory of Biology of Microorganisms and Biotechnology, We know that chemotherapy is associated with Univ. Oran 1 an increase in the formation of reactive oxygen [email protected] species and the depletion of plasma and these antioxidants. There are a limited number of studies that examine oxidative stress in patients Abstract undergoing chemotherapy. In this work, we try Oyster mushroom is specie of edible fungi of the to evaluate the diet in patients with NHL genus Pleurotus and a form of the family of undergoing first-line chemotherapy (CHOP) in a Pleurotaceae.This species has been the subject of retrospective study and the demonstration of an industrial culture for years and all over the metabolic disturbances of lipids and lipoproteins, world. In Algeria, these specie grow from autumn oxidative stress and the level of serum proteins. to winter in the north of the country and from Thirty new patients with NHL (16 men, 14 East to West. Several studies have shown thatit women, age: 54.30 ± 3.04) and thirty controls high consumption has an antitumor effect on (15 men, 15 women, age: 49.30 ± 2.12) were some cancer cells and prostate, however they

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have a very interesting nutritional value. In some P065 countries, these foods are a substitute for meat or fish .In our study, we are interested in the In Vitro Antioxidant, Anti-inflammatory and antioxidant activity of oyster mushrooms. The Cytotoxic Potential of Thyme (Thymus culture of the mycelium of the oyster mushroom vulgaris) Essential Oil and its Related strain is made under a specific physicochemical Terpenes on Tumor Cell Lines (Breast cancer condition in our laboratory. An fruiting bodies and Melanoma) (sporophores) is obtained at the cellars of the CFPA center of Messerghine (Oran). The obtained Mohamed Nadjib Boukhatem 123*, Noureldien H.E. fungi are subjected to an antioxidant activity test Darwish 1, Thangirala Sudha 1, Hanady Nada 1, Siham made with the DPPH method. In order to test the Bahlouli 2, Henni Chader 4, Yasmine Benali 5, Shaker A. ability of our fungi to directly trap the free Mousa 1 radicals. The activity is evaluated using a UV- 1 The Pharmaceutical Research Institute, Albany College of visible spectrophotometer which follows the Pharmacy and Health Sciences, Rensselaer, NY, USA. reduction of this radical that is accompanied by it 2 Département de Biologie et Physiologie Cellulaire, Faculté passage from the violet color to the yellow color; des Sciences de la Nature et de la Vie, Université – Saad the reading was repeated six times. The results Dahlab – Blida 1, Blida, Algeria. 3 Laboratoire Ethnobotanique et Substances Naturelles, Ecole are expressed as percent inhibition of the DPPH Normale Supérieure, Kouba, Alger, Algeria. radical. Trapping the free radical DPPH: The 4 Service Pharmaco-Toxicologie, Laboratoire National de antioxidant activity of our mushroom on the Contrôle des Produits Pharmaceutique, Dely-Brahim, Algeria. DPPH radical was evaluated using a 5 Laboratoire d'Anatomie et Cytologie Pathologiques spectrophotometer (at λ = 517nm) [5] .The Vétérinaires, Institut Pasteur d’Algérie, Algeria. [email protected] results are very interesting because they shown a significant antioxidant activity (EC50 = 6.83 mg / ml) higher than the ascorbic record acid (EC50 = Abstract 6.19 mg / ml). The EC50 values determined in mg/ml expressing the effective concentration of In recent years, essential oils have been reported the antioxidant extract necessary for the to possess interesting anti-tumor, anti-mutagenic entrapment and a reduction of 50% moles of and anti-carcinogenic activities against various dissolved DPPH in methanol. The consumption of cancer cells. Therefore, we investigated the in oyster mushroom can contribute to increase the vitro anti-inflammatory, antioxidant and intake of antioxidant compounds. In order to cytotoxic potential of Thymus vulgaris essential prevent cancer, it is therefore recommended to oil (TVEO) and some related terpenes (Thymol, have a diet rich in antioxidants. Further research Carvacrol and Linalool) on cancer cell lines. The is needed to identify, isolate and purify these cytotoxicity assay was done with MTT [3-(4,5- constituents. dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] to investigate changes in Keywords: Antioxidant activity; Oyster mitochondrial/non-mitochondrial mushroom; DPPH radical; cancer prevention. dehydrogenase activity and to determine the potential anti-proliferative property of the TVEO and related terpenes against two human cancer cell lines: Human Breast Adenocarcinoma (MDA-

MB 231) and Melanoma M3. Anti-inflammatory activity was assessed with in vitro (Human Red Blood Cell Membrane Stabilisation) and in vivo (xylene induced-ear edema) tests. The chemical composition of TVEO was determined with Gas Chromatography and revealed the presence of six compounds. Carvacrol was found to be the major component (77.4%). Linalool and Carvacrol were detected in minor quantities

(0.88% and 0.52%, respectively). Antioxidant activity was evaluated with a 2,2-diphenyl-1- picrylhydrazyl (DPPH) scavenging assay and

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metal chelating ions test. The percentage majority of the research community has inhibitions were concentration dependent with considered ALDH1A1 as a marker of cancer stem IC50 value of TVEO of 1.4 μg/mL scavenging cells and a predictor of the prognosis, this activity in DPPH free radical, while those of BHA enzyme also plays an important role in the and ascorbic acid were 0.44 µg/mL and 2.12 biology of tumors and cancer stem cells. The µg/mL, respectively. Topical anti-inflammatory objective of our studies is to identify an potential of TVEO was also explored in vivo and inhibitory molecule of the enzyme ALDH1A1, and exhibited a potent anti-inflammatory effect at all thus serve as an anticancer treatment. This study doses (100, 10 and 2 mg/kg), which were was carried out by means of simulation and statistically similar to the positive control molecular modeling, of which different software (Diclofenac Sodium). This activity was also was used. We quote among gaussian 09, confirmed at the cellular level with a chemsketch, marvensketch. Firstly, we have histopathology analysis. The MTT assay showed highlighted the structure of a series of molecules; that the TVEO exhibited significant dose- these molecules identified as potent inhibitors of dependent growth inhibition in the 50–200 mM the enzyme ALDH1A1. Secondly, we identify the dilution range. Carvacrol and Thymol showed descriptors of these molecules on the atomic, better results compared to Linalool, particularly energetic and physicochemical levels. Finally, we for breast cancer cells. Higher concentration of have established an equation that will serve as a Carvacrol and/or synergistic effect of the overall model of molecule that may be the best inhibitor composition were probably responsible for the of the enzyme ALDH1A1. From this series of efficacy of TVEO against the tested cancer cells. molecules and by means of simulation and TVEO may be a potential source of natural anti- molecular modeling, we come to highlight the cancer compounds and play an important role in equation that groups together the descriptors human health. and the characteristics of a molecule, which can have a better inhibitory effect on ALDH1A1 and Keywords: Anticancer Activity; Breast Cancer; thus will serve as treatment of Cancer. Prospects Melanoma; Thyme Essential Oil; Antioxidants. expected to study other molecules that can be used as anticancer treatment.

Keywords: ALDH1A1; gaussian09; simulation P066 and molecular modeling; treatment of Cancer.

Study and development of anticancer molecules by means of simulation and molecular modeling P067

Fatima zohra Fadel 1*, Noureddine Tchouar 2, Salah Laser Biophotonics for Cancer Diagnosis and Belaidi 3, Fatima soualmia2, Oualid Oukil2 Therapy (An Overview)

1 Department of Biotechnology, Faculty of Natural Science Ben Amar Cheba1, 2* and Life, University of Science and Technology of Oran -

Mohamed Boudiaf (USTO-MB) 31036, Algeria. 1 Biology Department, College of Science, Jouf University, P.O. 2Department of Chemistry-Physics, Faculty of Chemistry, Box: 2014, Sakaka, Saudi Arabia University of Science and Technology of Oran -Mohamed 2 Department of Biotechnology, Faculty of Nature and Life Boudiaf (USTO-MB) 31036, Algeria. Sciences, University of Sciences and Technology of Oran - 3Group of Computational and medicinal Chemistry, LMCE Mohamed Boudiaf (USTOMB), BP 1505 Al Mnaouar, Oran Laboratory, University of Biskra, BP 145 Biskra 07000, 31000, Algeria Algeria. [email protected] [email protected]

Abstract Abstract Over the past two decades, laser technology has The human genome encodes 19 aldehyde made great progress and explosive developments dehydrogenase (ALDH) enzymes that metabolize in the medical field as diagnostic, therapeutic, reactive aldehydes to their corresponding and surgical tools whereas in cancer biology the carboxylic acid derivatives. Although the

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laser technology provides new tools for both usefulness of medicinal plants in cancer academic and applied research in addition open occurrence to postpone cancer development or new eras and opportunities for future to delete side effects of chemotherapy. Other developments. Biophotonics, general term for all study suggests that may found treatment of techniques that deal with the interaction cancer in traditional medicine. Several natural between biological items and photons, this refers products have an anti cancer effects. This study to emission, detection, absorption, reflection, was carried out to investigate and explore modification, and reradiation from biomolecules, whether medicinal plants can be used as a cells, tissues, organisms and biomaterials, such treatment for breast cancer patients to improve a interactions form the basis of laser diagnostics in better quality of life. A survey of medicinal plants biology and medicine. Cancer biology as very for the treatment of breast cancer was carried fast growing discipline, with the rapid out in North West Algeria (Sidi Bel-Abbès). automation and the ultra fast laser spectroscopic Information on the plants names, parts used and techniques became multidisciplinary fields methods of preparation was collected through a which combine the mathematical, statistical , questionnaire among breast cancer patients. Our physical, chemical, biophysical, biochemical results found that 52% of breast cancer patients immunological ,serological, oncological, and used medicinal plants, among them 4% of molecular methods for cancer prediction, and population used commercial preparation and therapy ,even in single cells or in population 61% of them used cocking preparation and communities and their metabolites evaluation natural product such as curcuma, some patients for both clinical purposes or biotechnological found that some medicinal plants have a good applications. This review list all most different effect on cancer and chemotherapy treatment. laser spectroscopic approaches, and powerful Different species belonging to various botanical techniques, such as Laser-Induced Breakdown families were identified. Taken together, our Spectroscopy (LIBS), Laser Flow Cytometry findings suggest that medicinal plants are widely (LFC), Confocal Scanning Laser Microscopy used by breast cancer patients to avoid the side (CSLM), Laser Tweezers Raman Spectroscopy effects of chemotherapy treatment and improve a (LTRS) and Whole-Cell Mass Spectrometry (WC- better quality of life to survive. MS). Furthermore we review the photochemical principles and components with special focusing Keywords: Breast cancer; North West Algeria; on of Photodynamic therapy (PDT) and their Medicinal plants. utilities as powerful tool in cancer diagnosis and therapy.

Keywords: laser; techniques; PDT; cancer; P069 diagnosis; therapy. Anti-tumoral and anti-proliferative activities of bacteriocins from lactic acid bacteria

P068 Benmouna Zahia1*, Dalache Fatiha1,2, Zadi-Karam Halima1 and Karam Noureddine1. Traditional medicinal plants used by breast 1 Laboratory of Micro-organisms Biology and Biotechnology, cancer patients: results from a retrospective Department of Biotechnology, Faculty of Natural Sciences and study in Northern West Algeria Life, University of Oran1, P.B. 1524, El M’Naouer, 31000 Oran, Algeria. Fatima Zohra Hantour 1*, Bachir Benarba1. 2 Department of Biology, Faculty of Natural Sciences and Life, University of Mostaganem Abdelhamid Ibn Badis, P.B. 188, Laboratory Research on Biological Systems and Geomatics, 27000 Mostaganem, Algeria. University of Mascara, Algeria. [email protected] [email protected]

Abstract Abstract Human cancer is a major cause of mortality Recent studies proved that medicinal plants have worldwide. The heavy treatments used for the many virtues against cancer exploring the cancer therapy, the radiotherapy and the

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chemotherapy, cause a serious health problem. P070 That is why, the research of novel anticancer agents and modes of therapy are the subject of In vitro antioxidant and antiproliferative the several recent studies. A large number of properties of aqueous extract of Pistacia natural antimicrobial peptides (AMPs) have been Atlantica reported to display anticancer properties. The use of the bacteriocins as AMP from lactic acid Fatihabenahmed1,2, El Azhari Mehrab2,Wahiba bacteria to treat cancer has attracted several Rached3, Fatima Kerroum4, Mansouria Belmokhtar1, researchers, because of their safety for human Abdelkader Rouabhi5 And Omar Kharoubi1 consumption recognized by WHO (World Health Département de Biologie, Université Oran1 Ahmed Ben Bella Organization). The present study aims to 1, Oran, Algeria describe the ability of bacteriocins to treat 2 Département de Biologie, Centre Universitaire Relizane cancer, as reported in the literature. The data Ahmed Zabana, Algeria. were found through a research conducted on 3 Laboratoire Pharmacognosie et Api-Phytothérapie, Département de Biologie, Faculté des sciences de la nature et several works on the application of bacteriocins de la vie, Université Abdelhamid Ibn Badis, Mostaganem, in the therapy of cancer. The therapy of cancer by Algérie these molecules were based on four mechanisms: 4Biotechnology Research Center, Constantine 25000, Algeria direct killing, anti-inflammatory, wound healing 5Laboratoire traditionnelle de phytothérapie. Ain EL Beida Oran, Algérie. activities and immune modulation. In the recent [email protected] study, several researchers show the selective cytotoxicity effects of some bacteriocins on cancer cells. Already, some bacteriocins secreted Abstract from Enterobacteriaceae, such as the colicin and microcin E492, are known for their anti-cancer The medicinal plants may serve as potential activities against a variety of human tumor cell sources for the development of new drugs and lines in vitro, with no effect on normal cells. The more effective anticancer agents for future colorectal cancer, head and neck squamous cell therapy. Pistacia atlantica Desf (Anacardiaceae) carcinoma, are the kinds of cancers which could is an important plant used in traditional be treated using the bacteriocin therapy, like medicine practice in Algeria, and North Africa colicin and the bacteriocin produced by countries. The present study has the objective Pediococcus acidilactici K2a2-3. The nisin which to estimate the in vitro antioxidant (CUPRAC, is the first bacteriocin used as bio preservative, ABTS, DPPH) and antiproliferative (on the RD has shown to effectively reduce and prevent and Hep2 human tumor cell lines using the3- tumorigenic properties in vitro and in vivo. The [4,5-dimethylthiazol-2-yl]-2,5- field of using bacteriocins in cancer therapy is diphenyltetrazolium (MTT) assay) properties still new; further studies are imperative to of the aqueous extracts of Pistacia atlantica examine their clinical significance. The findings leaves, offering a phytochemical characterization presented suggest that this promising cancer of its aqueous extracts, by spectrophotometry therapy needs to be optimized and developed methods. Total phenolic contents (TPC) and further. flavonoid contents (TFC) of aqueous extract of P. atlantica indicated were demonstrated the Keywords: anti-cancer activity; bacteriocin; highest levels. The results of the studied extract lactic acid bacteria; therapy. were highlighted a higher antioxidant capacity. The MTT assay revealed that the tested extract had a good activity against Hep2 and RD cell lines with median inhibitory concentration (IC50) of 324.9198 µg/mL and 434.912µg/mL. The MTT assay revealed that the leaf aqueous extract of P. atlantica had the highest antiproliferative activity against Hep2 cells. The results showed a

great bioactive potential for this species with a significant contribution of phenolic compounds, specially the flavonoids which makes it an interesting matrix in the development of novel

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pharmaceutical formulations. Planned future scores of the α-synuclein / ligand complex. For studies will involve the identification of each ligand allowed us to highlight the most different extract other than aqueous extract, favorable mode of interaction. The results determination of the mechanisms of action and obtained show that for the majority of the ligands the bioactive molecule of plant extracts. the interactions are of the hydrogen or ionic type is the values of the calculated scores reproduce a Keywords: Pistacia atlantica; aqueous extract; descending order of the inhibitory power of the antioxidant, antiproliferative; phenolic six compounds VX-765> KYP2047> compounds; MTT. PAROXETINE> ANLE138b> NORTRIPTYLINE > SERTRALINE> NOCODAZOLE. Therefore, the high affinity of α-synuclein for the VX-765 and KYP2047 ligands is justified by the fact that they P071 correspond to it lower scores compared with that of nocodazole that is to say the least energetic Optimization in modeling of molecules for and the easiest enzyme / substrate complex. In inhibition of α-synuclein aggregation during addition, the VX-765 and KYP2047 show results multisystematous atrophy (MSA) comparable to the reference ligand which justifies their commercialization. The results 1* 1 Majda Dali-sahi , Nouria Medjati –Dennouni , obtained are particularly interesting in the Fouzia Mesli 2 , Said Ghalem 2. search for new drugs against multisystem 1 Laboratoire Chimie Analytique et Électrochimie Equipe atrophy. However, from the molecule to the drug, Biologie et Santé the steps are numerous, tests of affinity, 2 Laboratoire de recherché Substances naturelles et bioactives selectivity and toxicity, are required in this “LASNABIO” particular case or it will be necessary to abolish a [email protected] function to restore another.

Abstract Keywords: VX-765 ; KYP2047; α-synuclein ; PAROXETINE; ANLE138b. Multiple System Atrophy (MSA) is a rare, progressive, life-threatening neurodegenerative disorder beginning in adulthood, of uncertain P072 etiology, which clinically presents a variable combination of autonomic, parkinsonian, Application of virtual screening in the cerebellar, and pyramidal features. Α-synuclein is selection of new inhibitors of the Bcr-Abl primarily involved in the neuropathology of a group of neurodegenerative diseases called fusion protein, anticancer (drug candidates) "synucleopathies" which are: Parkinson's Mokhtari-Aitkaki Samira1*, Layachi Faycal1, disease, lewy-body dementia and multi-systemic Neghra Abdelhak 1, Kraim Khaireddine2, Ferkous atrophy. There is growing interest in molecules Foued 2 capable of reducing α-synuclein production, promoting accumulated synuclein clearance, and CHU Annaba, Laboratoire de chimie thérapeutique, faculté de increasing the stability of the native form to medecine- Annaba. Laboratoire de chimie organique appliquée, Université Badji avoid misfolding. Among the main categories of Mokhtar Annaba aggregation inhibitors that include proteins / [email protected] peptides and nanoparticles, the most interesting option is to discover or design new inhibitors of small molecules. Our approach is to optimize and Abstract model a better molecule that can inhibit Chronic myeloid leukemia is a cancer of white aggregation of α-synuclein with molecular blood cells. It is a form of leukemia characterized docking methods. The Enzyme / ligand by increased and unregulated growth of myeloid complexes have been realized with the MOE cells in the bone marrow and by the software. The scores (energies) are obtained in accumulation of these cells in the blood. CML was Kcal / mol. The interactions with the active site the first cancer to be associated with an obvious are visualized with MOE. Calculation of the genetic abnormality, the chromosomal 84 Oran 05-07 December

translocation known as the Philadelphia P073 chromosome. This is the result of a reciprocal translocation between chromosomes 9 and 22 Anticancer Drug Design: Development of resulting in the fusion of the BCR and ABL1 Tyrosine Kinase Inhibitors genes, which forms the BCR-ABL fusion gene. In our work, we focused on the treatment of CML Derouicha Matmour 1,2,4*, Yassine Mérad2, Yahia based on inhibition of the Bcr-Abl fusion protein; Dellaoui4, Fatima Zohra Hadjadj3, Houari Toumi4 we chose axitinib which is the most potent and 1Therapeutic Chemistry Laboratory, Department of selective third generation of the Bcr-Abl fusion Pharmacy, University of Sidi Bel-Abbes, 22000, Algeria. protein that binds to the active conformation of 2Central Laboratory, University Hospital Center of Sidi Bel- Bcr-Abl as conformation of DFG-in with residues Abbes, 22000, Algeria. Lys271, Met318 and Tyr253 buy hydrogen 3Therapeutic Chemistry Laboratory, Department of Pharmacy, University of Algiers, 16000, Algeria. bonds. Using molecular modeling methods, 4Research in Pharmaceutical Development Laboratory, especially docking by Autodock 4.2.6, Autodock Pharmacovigilance Department, University Hospital Center Vina and Molegro Virtual Docker, we evaluated 1st November, University of Oran, 31000, Algeria. the inhibitory activity for axitinib complex - Bcr- [email protected] Abl fusion protein "PDB: 4TWP" and similar axitinib downloaded from the PUBCHEM database. After molecular docking with the MVD, Abstract we drew the first 20 poses that represent the One of the limitations of conventional best interactions with Lys271, Tyr253 and chemotherapy is its lack of selectivity, altering Met318. We quote: 91207885, 134149925 and both tumor cells and healthy cells, resulting in 69371801. As for Autodock 4.2.6, we chose many adverse effects, sometimes limiting for among the first 20 poses those which represent further treatment. One of the proven drugs good binding energies; 22978316, 25181570 and against Chronic Myeloid Leukemia (CML), by 58890158. Similarly with Autodock Vina: inhibiting the protein activated by the Bcr-Abl 134142313, 134134141 and 134137011. To gene, is Imatinib, known commercially as reduce the number of molecules to be tested GLIVEC® from in silico molecular modeling. Our experimentally, we have filtered according to main objective is to identify new anticancer drug Lipinski's rule of 5 and according to the candidates, using the new technologies of obligatory presence of the three amino acids in structure-based drug design (SBDD) and the interactions. Four molecules 69371801, Quantitative Structure-Activity Relationship 69399014, 20781394, 90733463 selected by (QSAR). We used software of structure-based MVD were obtained, one of which, 69399014, drug design (SBDD) and Quantitative Structure- also ranked in the first 20 poses by Activity Relationship (QSAR) that is based on Autodock4.2.6. To conclude, we believe that the molecular modeling techniques and in silico MVD program is more suitable for virtual virtual screening. As a therapeutic target, we screening with our protein than Autodock 4.2.6 chose tyrosine kinase, which has been proven to and Autodock Vina, it better represents the key be a therapeutic target for the development of interactions governed by hydrogen bonds. targeted therapy and to overcome the problem of selectivity lack of chemotherapy drugs. Four Keywords: Molegro Virtual Docker; Autodock molecules that were chosen which IC50 are 4.2.6; Autodock Vina ; Docking; Bcr-Abl; Axitinib different; at each stage we chose the best in terms of interaction, binding modes, energy scores and predicted biological activity. The four molecules were compared to choose one. the molecule whose IC50 = 4 nmole, it proved the most refined, that means, that it presents the best interactions, the best energy score testifying the stability of the complex.

Keywords: Drug Design; Tyrosine Kinase; Molecular Modeling; Cancer.

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P074 P075

In Silico Studies of AXL Kinase Inhibitors Does Argan Oil affect Nitroso Compounds Formation? Derouicha Matmour 1,2,4*, Yahia Dellaoui4, Yassine Mérad2, Fatima Zohra Hadjadj3, Houari Toumi4 Fatima Zohra Chenni1 *, Gunter G C Kuhnle2 *

1Therapeutic Chemistry Laboratory, Department of 1 Department of Biology, Biotoxicology Laboratory, SNV Pharmacy, University of Sidi Bel-Abbes, 22000, Algeria. Faculty of Djilali Liabes of Sidi Bel Abbes University, BP 2Central Laboratory, University Hospital Center of Sidi Bel- 89,22000, Algeria Abbes, 22000, Algeria. 2 Department of Food & Nutritional Sciences, University of 3Therapeutic Chemistry Laboratory, Department of Reading, Reading RG6 6AP, United Kingdom Pharmacy, University of Algiers, 16000, Algeria. [email protected] 4Research in Pharmaceutical Development Laboratory, Pharmacovigilance Department, University Hospital Center 1st November, University of Oran, 31000, Algeria. [email protected] Abstract Recent epidemiologic, experimental, and mechanistic evidence suggest that dietary heme Abstract iron has a central role in the promotion of The tyrosine kinase of the AXL receptor and its colorectal cancer by promoting the formation of major ligand GAS6 (growth arrest specific gene N-nitroso compounds. However, diet rich in 6) have been shown to be overexpressed and fruits and vegetables may reduce the risk of activated in many human cancers (such as lung, colorectal cancer. Argan oil is among many breast and pancreatic cancer) and their natural products with several health benefits and activation has been demonstrated in many wide range compounds that are very beneficial. aspects of oncogenesis, invasion, metastasis and This oil takes origin from argan tree, endemic to resistance to targeted therapies. The objective of Morocco and Algeria. this work is to search for new drug candidates The aim of this study is to assess the effect of for lung, breast and pancreatic cancer treatment argan oil extracts on the modulation of the in using the molecular docking approach. We used vitro formation of ATNC (Apparent Total Nitroso software of molecular docking, we chose AXL as a Compounds), potent agents in the etiology of relevant therapeutic target and prediction colorectal cancer. In vitro incubations in software. Our practice is to study new AXL simulated gastric juice were conducted to assess inhibitory ligands with better affinity and the effect of polyphenols from argan oil on ATNC selectivity for this target. The virtual screening of formation. Different concentrations of argan oil 50 molecules belonging to the Can SAR Data Base extracts were incubated with bovine serum library brings out compounds of interest. The albumin, HCL 1M and 1.56 M of NaNO2 at 37°C. study of Molecular Docking of these molecules Samples were collected and analyzed by chemical allows to classify them according to their energy denitrosation with chemiluminescience detection score and to predict their interactions with active for different types of nitroso compounds 975. site residues while determining the The preliminary results of this study show that pharmacophore. The compound under the code argan oil has no relevant protective effect on the 56231879 presents a lower energy score is the in vitro modulation of ATNC compounds best. As prospects, it would be very interesting to formation, nitrosothiol and nitrosyl haem conduct structure_activity relationship studies to products. Although this findings suggest no predict the biological activity of this drug evidence of argan oil effect, this remains candidate. beneficial oil for human health and the great source of healthy dietary fat. Keywords: Molecular Docking, AXL Inhibitors, Cancer. Keywords: Nitroso Compounds, ATNC; Argan Oil; Simulated Gastric Juice; Cancer Colorectal.

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P076 medicinal plants benefit, as well as drugs, of a system of vigilance and awareness which Traditional medicine and cancer in Oran: prevents the risks of toxicity, interaction or what about interactions? inefficacity.

Zitouni H 1, 2,3 , Zitouni Haouar F.E.H 4, Fetati H1,2,3, Keywords: Cancer; Medicinal plant; Interaction; Mekaouche N1,2,3 , Memou A1,2,3,Senhadji I1,2,3 , Seddiki Oran. M 1,2,3 ,Toumi H1,2,3 .

1University Ahmed Benbella Oran I; Faculty of medicine, department of pharmacy. 2Service of pharmacovigilance; hospital center 1st November P077 Oran -EHUO- 3 Laboratoire De Recherché En Developpement Antioxidant and antiproliferative potential of Pharmaceutique LRDP. Syringa vulgaris L. flowers ethanolic extract 4 Laboratoire de biologie des microorganismes et de biotechnologie (LBMB), Oran Algeria 1,2* 2 2 [email protected] ; [email protected] Emoke Pall , Spinu Marina , Niculae Mihaela , Koula Doukani3, Adriana Corina Hangan4, Roxana Liana Stan5

Abstract Life Science Institute, University of Agricultural Sciences and The last decade has seen an increase in the use of Veterinary Medicine, 400372, Cluj-Napoca, Romania. Department of Infectious Diseases, University of Agricultural traditional medicine especially in cancer patients Sciences and Veterinary Medicine, 400372, Cluj-Napoca, with utilization rates of up to 88%. The interest Romania. in this topic and the number of publications Faculty of Nature and Life Sciences, University of Ibn continues to grow; however the majority of Khaldoun-Tiaret, 14000, Algeria 4Department of Inorganic Chemistry, Faculty of Pharmacy, studies are Anglo-Saxon, there are few studies in University of Medicine and Pharmacy “Iuliu Hațieganu”, Algeria regarding the use of medicinal plants in 400000, Cluj-Napoca, Romania. cancer patients. It there for seemed useful and 5Department of Pharmaceutical Biochemistry and Clinical important to draw up an inventory of the Laboratory, Faculty of Pharmacy, Iuliu Hatieganu University practice of traditional medicine in cancer of Medicine and Pharmacy, 400000, Cluj-Napoca, Romania. [email protected] patients in the city of Oran. Our objectives are: to list the medicinal plants used against cancer, describe the characteristics of this use, the Abstract profile of the users and estimate the drug –plant risk of interaction. This is a cross-sectional Syringa vulgaris L., (common lilac) belongs to the descriptive study carried out from 26 November Oleaceae family and is regarded as one of the 2015 to 10 January 2016, within the EHUO most popular ornamental plant, but also as an oncology services as well as the EHS Emir AEK interesting choice in terms of antioxidant, anti- Oran. The survey was conducted using a inflammatory, anti-nociceptive and antipyretic questionnaire for 384 patients. Data and properties. Similarly, phytochemical studies on statistical analysis were done on SPSS and Excel Syringa species have pointed out the presence of softwares. The analysis of the survey data bioactive secondary metabolites with anti- identified 46 vegetal drugs used for traditional oxidant and anti-inflammatory activities. Our cancer treatment, which many have interactions study was designed to assess the antioxidant and with anticancer drugs. Our results show that antiproliferative property of an ethanolic extract 43% of the population studied are women with obtained from Syringa vulgaris L. flowers. The breast cancer, among the 384 cancer patients, protective effect of the ethanolic extract against 47.4% use at least one medicinal plant. The use free radicals and in vitro SOD like activity was of medicinal plants differs significantly with the evaluated. The citotoxicity and antiproliferative sex with a P ≤ 0.003 with a female predominance potential of ethanolic extract were investigated or a frequency of 75%. We also found that 39.4% on human keratinocyte (HaCaT) and murine of patients use medicinal plants in combination melanoma (B16F10) cell lines using CCK8 and with their treatment and that 26.4% of patients MTT assays. Our results indicated the Syringa can experience interactions following this vulgaris ethanolic extract ability to efficiently association. In conclusion, it is imperative that protect against superoxide anions, therefore it

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can be considered as a promising effective agent Antioxidant activities of hydrolysates were against toxicity of superoxide anion. CCK8 and evaluated by 2,2-diphenyl-1-picrylhydrazyl MTT tests indicated the antiproliferative (DPPH) and Nitric oxide (NO) free radicals potential of Syringa vulgaris extract was scavenging methods. Whey proteins hydrolysed expressed in a dose dependent manner, without by the strains LBM2 and LBBS2 showed the negative effect on normal cell line. Syringa highest antioxydant activity with both the vulgaris extract displayed citoxicity potential and methods used compared to samples obtained by increased SOD-like activity and based on these the strain (CHTD27). Our findings suggest that results it may be considered as a potential whey proteins hydrolysed by strains of therapeutic agent for the prevention and Lactobacillus could potentially be used for treatment of radicals mediated diseases free by. producing novel functional foods. However, the Both authors contributed equally to this work. development of antioxidant activity was strain- This work was additionaly supported by the specific characteristic. The strains Lactobacillus Domus Hungarica Scientiarum and Artium plantarum LBM2 and LBBS2 could be used as research fellowships. new potential adjunct bacteria. Moreover, whey could be used for the production of molecules Keywords: Syringa vulgaris, ethanolic extract, with a promising interest as antioxidants. antioxidant, antiproliferative. Keywords: antioxidant activity, hydrolysates, Lactobacillus, whey proteins.

P078 P079 Antioxidant activity of whey protein Theoretical contribution of bastadins: hydrolysates obtained by strains of molecules of therapeutic interest Lactobacillus

1* 2 3 1* 1 Nabila Taib , Nourreddine Tchouar , Salah Belaidi Hayet Messaoui , Salima Roudj , Noureddine 1 Karam . 1 Départment of Biotechnology, Laboratory of Modeling and Optimization of Industrial Systems, Faculty of Natural Laboratoire de Biologie Des Microorganismes et Sciences and Life, University of USTO-MB, BP 1503 Oran Biotechnologie 1. 31000, Algeria [email protected] 2 Department of Chemistry Physics, Laboratory of Modeling and Optimization of Industrial Systems, Faculty of Chemistry, University of USTO-MB, BP 1503 Oran 31000, Algeria Abstract 3 Group of Computational and Pharmaceutical Chemistry, Laboratory of Molecular Chemistry and Environment, Faculty Reactive oxygen species (ROS) and free radicals of Chemistry, University of Biskra, BP 145 Biskra 07000, play an important role in many degenerative Algeria * diseases like cancer, atherosclerosis and [email protected] diabetes. These radicals are very unstable and react rapidly with other groups or substances in Abstract body, leading to cell or tissue injury. Foods containing antioxidative materials may be useful The natural environment has always been a very for the prevention of these diseases. Antioxidant important source of molecules with anti- activity is one of the multiple health-promoting angiogenic activity, natural products have attributes assigned to bovine whey products. inspired many developments in organic Hence, the aim of this study was to investigate chemistry, particularly leading to advances in the antioxidant activity of whey proteins hydrolysed methodologies of synthesis and the ability to by strains of Lactobacillus. Whey protein make analogs with properties pharmaceutical or hydrolysates were prepared using two strains of pharmacological improved, many natural Lactobacillus plantarum (LBM2 and LBBS2) products and synthetic analogues have been isolated from raw ewe’s milk and one strain of successfully developed for the treatment of Lactobacillus brevis (CHTD27) from camel milk. cancer, in this work we focus on the theoretical

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study of bastadins (bis-diaryl), this family of with stress, pain and anxiety. Emotional Freedom naturally occurring molecules is produced by Techniques include a variety of practices that marine sponges and has anti-angiogenic generally involve tapping acupuncture points properties. The objective of this work is to model while repeating statements that focus on a the bastadin 6 molecule and some of its natural specific issue, on such as anxiety or addiction, and synthetic analogs and establish a correlation technique which helps to experience emotional between energy properties and anti-angiogenic harmony and is based on tapping acupuncture properties of these products. The calculations points heal. Our study was quasi experimental to were made using the modeling and molecular evaluate the effectiveness of Emotional Freedom simulation software: Gaussian 09 based on the Techniques on anxiety by cancer. Investigator DFT method "density functional theory". The found that the Emotional freedom technique is results show that: effective in decreasing the level of anxiety.  The bastadin 6 molecule is the most stable molecule by contributing to its natural and Keywords: Cancer, Emotional freedom synthetic analogues. techniques, Anxiety, Decreasing.  The natural product is the best therapeutic product  The anticancer activity of these molecules P081 increases with the stability and HOMO- LUMO energy difference. Propolis extract reduces prostate cancer cells  The biological activity of bastadins depends proliferation in Wistar rat on two oxime functions locating in the right part of the molecule. Nada Zabaiou 1*, Mesbah Lahouel 1, Jean Marc A.  The presence of bromine give to the Lobaccaro 2,3,4,5. molecule a good rigidity In this work, we have shown that the modeling of Laboratory of Molecular Toxicology, Department of natural anti-angiogenic molecules to describe Molecular and Cellular Biology, Faculty of Sciences, University of Jijel, 98 Ouled Aïssa 18000 JIJEL, Algeria. biological systems to remove information Université Clermont Auvergne, Université Blaise Pascal, GReD, difficult to obtain experimentally and synthesize 28, place Henri Dunant, BP38, F63001 CLERMONT-FERRAND. chemicals with improved pharmaceutical CNRS UMR 6293, GReD, 10 avenue Blaise Pascal, Campus properties. Computer science as a research tool Universitaire des Cézeaux, CS60026, F-63178 AUBIERE. is growing and the research topic presented here INSERM UMR 1103, GReD, 10 avenue Blaise Pascal, Campus Universitaire des Cézeaux, CS60026, F-63178 AUBIERE. shows only a tiny part of the current Centre de Recherche en Nutrition Humaine d’Auvergne, 58 development of computer science. Boulevard Montalembert, F-63009 CLERMONT-FERRAND. [email protected] Keywords: anti-angiogenic; bastadin; cancer; Abstract energy. Prostate cancer is a very heterogeneous disease, in terms of its pathology and its clinical presentation. Localized prostate cancer can be P080 effectively treated by surgery or radiotherapy. In advanced stages, androgen deprivation is the Emotional freedom techniques for cancer best therapy. Unfortunately, after a short time, androgen-independent cancer, very aggressive Keltoum Dermeche, Asma Tamert and resistant to conventional hormonal

1University center Ahmed Zabana, Department of Biology, therapies, develops. It is therefore essential to Institute of sciences of nature and life, Rélizane 48000, develop new molecules, of natural origin, more Algeria. [email protected] active, to reduce costs and allow greater accessibility for a larger number. The aim of this work is therefore, to study the anti-proliferative Abstract effect of the ethanolic extract of propolis on Cancer patients, such as those diagnosed with chemical-induced prostate cancer in Wistar rats. malignant mesothelioma, may use EFT as an Benzo (a) pyrene [B (a)P], a chemical molecule alternative or complementary therapy to deal belonging to the PAH (Polycyclic Aromatic

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Hydrocarbons) family was administred P082 intraperitoneally to Wistar rats and then, after 28 weeks of treatment, the rats were treated with an Evaluation of protective effects of opuntia ethanolic extract of propolis (20 mg / kg) ficus-indica cladodes on colorectal cancer administered by gavage for 21 days. We studied the histological modifications by H/E staining. 1Hanane Dib, F.Z Ghanemi, Zoubida Soualem, Meriem The Ki67 proliferation marker was studied by Belarbi, 2Aziz Hichami and Naim Khan. immunohistochemistry and the expression of the 1Researche laboratory “Natural products”, Faculty of SNV- AhR receptor by RT-qPCR and western blot. STU Abou-bekr Belkaid,Tlemcen, Algeri Intraperitoneal injection of B (a)P induces 2INSERM U866, Dijon, France. prostate cancer in Wistar rats. B(a)P, in itself, is a [email protected] procarcinogen. However, it acts as a carcinogen in animals when activated after its metabolism and causes gene mutations by binding to DNA. Abstract This is called initiation of carcinogenesis caused Obesity has been referred to as the epidemic of by B (a)P and the phase characterized by the the 21st century. Recent epidemiological studies growth of irreversibly transformed cells is called on patients with obesity or type 2diabetes have tumor promotion. Oral administration of revealed higher risks for several kinds of cancer, propolis to Wistar rats resulted in a decrease in including breast, colorectal, pancreatic, and liver. cell proliferation and a 49% reduction in Ki67 In addition, oxidative stress may be a linking expression. Already studied on mice with factor between obesity and cancer. Recently, melanoma B16F1 tumors, this propolis increases functional foods are the subject of numerous mouse survival by 30% and reduction of the studies because in addition to their high mitotic index (-75%) and Ki-67 (-50%). Many nutritional value, they offer an additional health studies have already studied the effect of benefit. The cactus and cladodes of Opuntia ficus- propolis on the development of prostate cancer. indica are widely consumed in the Mexican diet. These studies showed that propolis acts on the It has a low glycemic index and is considered a proliferation of prostate cancer cells of different functional food due to its high content of dietary lines generally studied by the MTT test, but the fiber and polyphenols concentration. Phenolics molecular mechanisms involved are very poorly are an important class of secondary plant described. Our propolis extract reduces the metabolites possessing an impressive array of expression of the AhR nuclear receptor at the pharmacological activity. Moreover, several transcriptional and protein levels. It has been reports suggest that the polyphenolic compounds shown that the consumption of food rich in AhR from fruits and vegetables could suppress cancer ligands leads to the triggering of the AhR through their antioxidant, antiproliferative and activation cascade. The signal transduction of proapoptotic properties. In this study, we natural AhR ligands differs from that of toxic investigated the protective effect of dietary molecules such as dioxin. Park et al. have shown cactus Opuntia ficus-indica cladodes (OFIC) on that the ethanolic extract of Brazilian propolis the oxidative stress and the incidence of containing high flavonoid concentrations colorectal cancer, induced by N-Methyl- suppresses the transformation of AhR induced by Nitrosurea (NMU) in diet-induced obesity. its toxic ligand TCDD, a toxic synthetic agonist of Phenolic compounds were extracted, and total AhR. Our propolis extract has a very strong anti- polyphenols, flavonoids and tannins were proliferative effect against B(a)P induced assayed. Antioxidant parameters were evaluated prostate cancer. A complementary study of the by colorimetric methods. Wistar rats were fed for mechanisms involved seems to be essential. 4 weeks with a control or a high-fat diet, enriched or not with OFIC (50%), then colorectal Keywords: Prostate cancer; Benzo(a)pyrene; cancer was induced by intraperitoneal (.i.p) Propolis; Wistar rat. injection of NMU (50 mg/kg body weight).The oxidative stress was monitored in plasma by measuring the MDA level, the carbonyls protein generation, CAT, SOD, GSH-Px and GSSG-Red

activities and evaluation of antioxidant status by ORAC. BALB/c mice were subcutaneously

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implanted with CT26 colon carcinoma cells, and cost of production remains really expensive then were .i.p injected with (OFIC) polyphenols because of research and manufacturing process. (50 mg/kg/d) during 7days. Our results clearly In USA, a research report from the Business showed that obesity and NMU induced significant Communications Company (BCC) showed that alterations in oxidative stress markers. the global nanomedicine sector, was worth $100 Conversely, the cactus cladodes diet improved billion in 2014, where anticancer products the oxidative stress and reduced the number of valued about $33 billion. To overcome this colon polyps induced by NMU/obesity. problem, the pharmacoeconomics has been used Furthermore .i.p. administration of OFIC as a tool to help health care decision-makers to polyphenols reduced CT26 tumor growth. determine effectiveness cost of the therapy. The Dietary Opuntia ficus-indica cladodes (OFIC), by aim of this work is to show the their content of polyphenols, may improve benefit of pharmacoeconomics by choosing oxidant stress and decrease the risk of colorectal nanotherapy as an economic treatment for cancer, associated with obesity. cancer compared to conventional treatment cancer in limited ressources coutries. The work Key words: colorectal cancer, CT-26, Opuntia is a literature review formed on scientifics ficus-indica, cladodes, phenolic compounds. articles and many wibesites and research motors where used as: Pub med, Springer, science direct. According to many research published, biotechnology using nanoparticule fight cancer in P083 a better way than classical chemical drugs. Biotechnology target cancer cells far more Pharmacoeconomics and nanotherapy in precisely and with less overall damage than in cancer treatment conventional therapy. For example, Doxil (PEGylated liposomal formulation of Krid M.1*, Djelad Sanaa B.1, 2, Rezk-Kallah H.1, 2. doxorubicin) using in metastatic breast cancer. In

Pharmacology toxicology departement, university hospital of this study, 22,509 women suffert of cancer are Oran, Algeria treated with doxil or doxorubicin. These therapy Health and enviromental research laboratory, university of caused cardiotoxicity as effect side. Comparing Oran 1, Algeria. the risk of cardiotoxicity with doxorubicin was [email protected] significantly higher than Doxil. These new Abstract: treatments remain hard to master and their coast Despite recent progression in detection and seem extremely high in beguining but at long therapy, cancer is remaining a major health care term represent an interesting economy by problem. The number of patients suffering from decreasing hospitalization cost and complication. it has been estimated by the Algerian registries of For exemple, comparing gemcitabine and cancer at nearly 45,000 cases since 2015. In PEGylated liposomal doxorubicin for ovarian countries where resources are limited, the cost of cancer. Cost with gemcitabine treatment was cancer treatment is an issue with significant significantly higher than nanotherapy with economic repercussions. Treatment success is 5,547.30 dollars resulting for one patient in a mainly limited due to difficulties in diagnosis difference of 3,350.28 dollars for nanotherapy. besides the cancer presentation is often late. ,Pharmacoeconomics is increasly used in Chemotherapy and radiotherapy are developed countries as tool in choosing cancer conventional treatment of cancer. The problem treatment and improving quality of therapy to with this therapy is the lack of specificity to decrease toxicity and side effects and finally to target cancer cells. To answer this problem, minimazing cost. However, this science of multi-target strategies have been developed economy is still limited in poor contries where instead to identify effective diagnosis and ressouces is limited. treatment such as nanotherapy using nanotechnology. In this therapy, nanoparticles Keywords: nanoparticle, cancer, therapy, (NPs) are used for drug delivery, for in vitro pharmacoeconomics. diagnosis and in vivo imaging wich permet to target cancer cells, to increase effeciency and decrease side effect and toxicity. However, their 91 Oran 05-07 December

P084 phosphorylation, caspase-8 and caspase-9, which is an essential downstream component of p53, Polyphenols from Pennisetum glaucum activation. Indeed, PGPC triggered pro-caspase-8 grains induce caspase-mediated apoptosis in and pro-caspase-9 cleavage into their active human osteosarcoma cells fragments. Furthermore, PGPC induced PARP cleavage, a downstream substrate of both Abdelhafid Nani 1,2,3*, Ikram Aboura2,3, Fatima Zahra caspase-8 and caspase-9. In response to ER Ghanemi2,3, Meriem Belarbi3, Taha Merghoub4, stress, inositol-requiring enzyme 1 (IRE1) Mickaël Rialland3, Naim Akhtar Khan3, Aziz Hichami3. mediates a pathway known as unfolded protein response (UPR) in which gene expression is Department of Natural and Life Sciences, African altered. However, severe ER stress can lead to University Ahmed Draia, Adrar 01000, Algeria. Laboratory of Natural Products, University of Abou- programmed cell death. Moreover, alteration in Bekr Belkaid, Tlemcen 13000, Algeria. calcium homeostasis, splicing of X box- U1231 INSERM/Université de Bourgogne-Franche bindingprotein 1 (XBP1) mRNA, and increased Comté (UBFC)/Agro-Sup, Physiologie de la Nutrition & CHOP expression are usually described in ER Toxicologie, Dijon 21000, France. stress-mediated apoptosis. Thus, p38 MAPK, Department of Medicine, Memorial Sloan Kettering upstream component of p53, has been reported Cancer Center, New York, NY 10065, United States. to downregulate Bcl-2 survival protein via CHOP, [email protected] a highly stress-inducible gene, activation. PGPC treatment increased transcription of XBP1, BAX/ BAK, and CHOP mRNA expression, and decreased Abstract Bcl-2 mRNA expression in U-2OS cancer cells. We Osteosarcoma is the most common bone tumor deduce that PGPC induce caspase-dependant with a high prevalence among children and apoptosis through p53 activation. Overall, this adolescents. Polyphenols are widely investigated study encourages the exploration of pearl millet for their chemopreventive and chemotherapeutic polyphenols as a promising candidate for proprieties. In the present study, we investigated osteosarcoma prevention and treatment. the pro-apoptotic effects of pearl millet, Nonetheless, anti-tumor effects in animal models Pennisetum glaucum, phenolic compounds remain to be investigated. (PGPC) on osteosarcoma U-2OS cells. Pearl millet phenolic extracts were obtained according to the Keywords: Apoptosis; pearl millet; polyphenols; method of Liyana-Pathirana and Shahidi with U-2OS. slight modifications. Apoptosis determined by

APC-Annexin V and 7-amino-actinomycin D (7- AAD). P-p53, p53, caspase-8, caspase-9 and P085 cleaved PARP activation was assessed by western blot. Bcl-2, XBP1, BAX/BAK, and CHOP Chimeric Antigen Receptor T-cell (CAR -T) mRNA expression was assessed by RT-qPCR. Our Therapy for Hematologic and Solid findings demonstrate that PGPC induced cell Malignancies: Risk and benefits- A Systematic death mainly via apoptosis as shown by a Review with Meta-Analysis significant decrease in cell death when cells were co-treated with z-VAD-fmk, a pan-caspase Imene Fekhikher 1*, Raouida Mecifi 2 , Youcef inhibitor. The extrinsic, or death-receptor Bouali Youcef1 pathway, and intrinsic, or mitochondrial pathway are the most described pathways of the apoptosis 1Departement of immunology EHU 1st November , Oran , Algeria. machinery. In most human cancers, the p53 – 2 Cancer treatment centre.Chetouane, Tlemcen ,Algeria. tumor suppressor– protein or pathway is 3 Departement of immunology EHU 1st November Oran , mutated or inactivated. We and others have Algeria. shown that p53 can mediate caspase-dependent [email protected] apoptosis, and many stimuli, including polyphenols, trigger apoptosis through the Abstract activation of initiator caspases (caspase-8 and caspase-9). In this study, western blot showed Chimeric antigen receptors T cells (CAR -T) had that PGPC treatment induced p53 been used for treating various tumor patients in

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clinic, and owned an incredible efficacy in part of P086 malignancies. However, CAR -T therapy remains controversial due to doubts about its efficacy and LRRK2 Protein Inhibitors During Parkinson's safety in the clinical treatment of various Pathology malignancies. Our objective was to summarize the efficacy and safety of CAR-T cell therapy. Ikram chebiebe1*,NourredineMissoum2,Majda Dali- Interventional studies investigating CAR-T cell sahi1, Medjati-DennouniNouria1, Said Ghalem2 therapy in patients with malignancies were 1AnalyticalChemistry and included. Our primary outcome of interest was ElectrochemistryLaboratoryBiology and Health Team 1. complete response (defined as the absence of 2Research laboratory Natural and bioactive detectable cancer). A total of 42 hematological substances“LASNABIO” malignancy studies and 18 solid tumor studies *[email protected] were included (913 participants). Of 486 Abstract evaluable hematologic patients, 54.4% [95% CI, Parkinson's disease (PD) is the second most 42.5%-65.9%] experienced complete response in common neurodegenerative disorder in the 27 CD19 CAR-T cell therapy studies. Of 65 world. At the cellular level, it is characterized by evaluable hematologic patients, 24.4% [95% CI, a progressive loss of dopaminergic neurons (DA) 9.4%-50.3%] experienced complete response in in the Black substance pars compacta as well as seven non-CD19 CAR-T cell therapy studies. cytoplasmic protein aggregates (Lewy body) in Cytokine release syndrome was experienced by surviving neuronal cells. The exact etiology is not 55.3% [95% CI, 40.3%-69.4%] of patients and yet completely clear. Mutations in the leucine- neurotoxicity 37.2% [95% CI, 28.6%-46.8%] of rich repeat kinase 2 gene (Lrrk2) have been patients with hematologic malignancies. Of 86 evaluable solid tumor patients, 4.1% [95% CI, shown to be the most common cause of both hereditary and idiopathic cases. The G2019S 1.6%-10.6%] experienced complete response in mutation within this gene increases the kinase eight CAR-T cell therapy studies. CAR T cells with activity of the LRRK2 protein, contributing to the higher doses and longer persistence in vivo can pathogenesis of Parkinson's disease. The actual lead to better response rates however these function of the LRRK2 protein is not yet clear, promising results have been tempered by safety although it has been implicated in several considerations, including cytokine release pathways, including synaptic vesicle regulation, syndrome, neurotoxicity, and other rare and endocytosis and membrane trafficking, as it can uncommon adverse events. These results will also interact with other proteins involved in help to inform patients, physicians of the benefits parkinson's disease such as alpha synuclein. We and risks associated with CAR-T cell therapy. propose in this work: to inhibit the kinase domain of lrrk2 protein via a series of inhibitors Keywords: CART; cytokine release syndrome; and to predict the interactions between lrrk2 and effeciency; neurotoxicity. alpha synuclein. In our study we used MST3 as a powerful model thatshares a 73% homologywith the kinase domain of LRRK2. The results show that the ligand (4) of the family (Quinolines) represents the best inhibitor, wecanalsoconcludethat the family of Quinolinesis best placed to inhibit LRRK2. It has been verifiedthat LRRK2 has asignificantability to phosphorylate alpha synuclein (Asyn) at serine 129. The Dockingresults show thattheremaybe 3 MST3 interactions (Met13, Pro11, Gly9) with Alpha synuclein (Thr92, Lys96, Asp98)

respectively.

Keywords: Parkinson 1; a-syn, LRRK2 2; Black substance pars compacta dopaminergic.

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P087 electronic scanning microscopy, FTIR, TGA and rheological tests. The stability of curcumin from Synthesis and characterization of hydrogel films at light and different pH values was films based on polysaccharide and their evaluated. The oxidation degree of CMC-Ox applications in the treatment of skin cancer varies between 25-45% and is influenced by the sodium periodate amount used. The amount of Mohammed DELLALI1,2 , Camelia Elena IURCIUC amino groups that did not participate at cross- (TINCU)2, Kheira ZANOUNE DELLALI1,2 ,Nawel linking (determined by the ninhydrin test) SPAHIS1 , Marcel POPA2,3 decreases as the CMC-Ox amount increases as a result of numerous amino groups from chitosan / 1 Hassiba Benbouali University of Chlef, Algeria, 2Gheorghe Asachi” Technical University of Iasi, Romania, lactalbumin. The duration of the crosslinking 3Academy of Romanian Scientists, Splaiul Independentei 54, process (up to 3 hours) determines the increase Bucuresti, Romania of the crosslinking degree (in accordance with [email protected] the decrease of free amino groups), respectively the decrease of the swelling degree values in different mediums. The maximum crosslinking Abstract degree is reached at 30⁰C. It has been found that Turmeric (Curcuma longa), a spice commonly the polymeric matrix has a protective character used throughout the world, has anti- for curcumin, both in light and in solutions with inflammatory, antimicrobial, antioxidant and different pH values. The curcumin release anti-neoplastic properties and has been kinetics from films was studied in two mediums successfully used to treat rashes and skin of different pH (5.5 and 7.4) and it was found infections. The main disadvantage is its water higher release efficiency at pH 7.4, the maximum insolubility and low bioavailability. Inclusion efficiency being 70% after 48 hours; the results complexes with β-cyclodextrin to increase its are consistent with the swelling degree. The water solubility have been obtained, these being results obtained led to the following conclusions: subsequently immobilized in co-reticulated (i) CMC-Ox could constitute an appropriate hydrogel films based on polysaccharides cross-linking agent for chitosan or for its mixture (chitosan, oxidized carboxymethyl cellulose) and with α-lactalbumin, also having the advantage to proteins (α-lactalbumin) in order to treat avoid the use of another covalent cross-linker, especially dermatological diseases. The chitosan usually toxic; (ii) curcumin allows to obtain used was of medium molecular weight, with a complexes with with β-cyclodextrin, thus degree of deacetylation of 75%. Crosslinking to improving its water solubility; (iii) curcumin obtain hydrogels was performed with partially complexes can be immobilized successfully in oxidized carboxymethyl cellulose (CMC-Ox) with films with hydrogel character based on chitosan NaIO4, to form aldehyde groups by cleaving the and α-lactalbumin, leading to systems with C2-C3 bond. The oxidation degree was between increased bioavailability; (iiii) the polymeric 25-45% being influenced by the amount of matrix of the films has a protective role for oxidant used. α-Lactalbumin (milk-derived curcumin against degradation at light or in protein ) was used on the one hand for the mediums of different pH. solubilization of the curcumin / β -cyclodextrin complex in the aqueous synthesis medium of the Keywords: Skin cancer; Hydrogel film; hydrogels, but also to participate to the polysaccharide; Treatment formation of hydrogels. The films were obtained by crosslinking as a result of the reaction of the chitosan and α-lactalbumin amine groups with the aldehyde groups in CMC-Ox, working at different molar ratios -NH2 / -CH = O (1: 0.25, 1: 0.375, 1 : 0.5, 1: 0.75, 1: 1). The inclusion complex (150 mg) solubilized in lactalbumin solution (1%, 5 ml) was included in the chitosan- CMC-Ox films from the preparation stage. The films obtained were characterized by the determination of the swelling degree, by

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Cancer diagnostic technologies, Immunotechnology and Cancer Immunotherapy

95

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P088 P089 Association between clinicopathological Detection of papillomavirus (HPV) infections characteristics and KRAS mutation in at the cervix in women at risk (HIV-positive) colorectal cancer by a method of molecular biology: classic PCR

Afaf BENHOUDA1*, Hachani KHADRAOUI 2,Rania Dalale Behar 1*, Lamia Boublenza 1, Nabila Masdoua BENBOUZA2 ,Massinissa YAHIA 1, Atika SOUSSI 1, Wafa 1, Nafissa Chabni 2. KHETTACHE 1 . 1LAMAABE, Department of Biology, University of Tlemcen. *1 Biotechnology’s Laboratory of the Bioactive Molecules and 2Epidemiology Service, CHU -Tlemcen. the Cellular Physiopathology, Faculty of Biological Sciences, [email protected] Department of Biology of organisms, University of Batna 2, Algeria. Abstract Laboratory of anapathology Center anti Cancer BATNA- HIV-related immunosuppression for a variety of Algeria [email protected] reasons is a significant risk for persistent infections with human papillomavirus (HPV), Abstract which increases the risk of having complications Colorectal cancer is a major public health and cancers compared to the general population. problem. It is the most common cancer in The goal of our work is to detect the presence of Algeria. Among the most common prognostic HPV DNA from cervical samples using the factors for CRC is mutation status analysis, which classical PCR technique and the Amplicor® kit. A is one of the most common genetic alterations in total of 32 samples is the subject of HPV DNA this pathology. Previous studies have shown that detection. HPV / HIV-1 coinfection was found in KRAS mutations are a predictor of the response 24 (75%) patients, of whom 17 (70.8%) had to EGFR-targeted therapies in patients with intraepithelial lesions and 7 had inflammations, metastatic colorectal cancer. the remaining 8 HIV-positive women being HPV- The aim of our work is to compare the negative. According to this study, we aknowledge mutational status of the KRAS gene, and to know that the frequency of HPV infection was if the mutations correlate with the anatomo- significantly high in HIV-1 positive patients clinical behavior and prognosis of CCR patients in which is in accordance with the literature, Seen BATNA-Algeria. Our retrospective study which that several researchers have noted a high consisted of 91 cases, 46.2% of patients are prevalence of cervical abnormalities in women found affected by the KRAS mutation, and 58.3% infected with HIV-1, with rates of 40%. HIV are wild-type KRAS. The KRAS mutations were infection is associated with gynecological risks detected by RT-PCR and confirmed by (cancer of the cervix, vulva ...), which is why it is sequencing where they found that codons 12 and recommended to systematically offer HIV- 13 of exon 1 and exon 2 of the gene were the infected women screening for HPV-induced most affected. Mutations p. G12D (c.35G> A) and lesions through the implementation of HIV p.G13D (c.38G>A) were the most frequently infection. a cervical smear at the time of the detected mutations. KRAS mutations were discovery of the seropositivity as well as the independently variable age, sex, tumor size, type possibility of genotyping the HPV, which is a of metastasis, appearance histological, tumor technological progress whose interest in the localization, neoplastic emboli and stage PTNM. diagnostic or even therapeutic steps, deserves to be discussed. Keywords: CCR, mutated KRAS, wild-type KRAS, mutations, Batna, Algeria. Keywords: HPV, HIV, cervical sampling, PCR, cancer.

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P090 study to enhance the staining technique. This modified method will help the pathologist to Comparison between Modified Papanicolaou exhibited easier diagnosis of keratinization in Stain and Hematoxylin–Eosin Stain for the samples of oral squamous cell carcinoma, since it Demonstration of Keratin in Oral Tissue gives different colour contrast to the background Sections and the keratin. Hence, allowing easy identification of keratin and more accurate Nusyba Mohamed Ahmed Bushra Mohamed diagnosis. Also such a technique can be adapted 1 1 Ahmed *, Ramy Yousif Hasab Elrasul Mohammed , for precise diagnosis of other tissues that form Nazik Omer El-bashir2 keratin in the presence of cancer, which can in

1 Department of Histopathology, University of Medical turn help determine the appropriate Sciences and Technology; P.O. Box 12810 Khartoum, treatment. Moreover, such technique can be used Sudan to teach student and medical laboratory 2 Department of Oral Pathology, University of technologist how to enhance the staining Khartoum; P.O. Box 102 Khartoum, Sudan technique in terms of specificity and intensity [email protected] when staining oral tissue section suspected to Abstract have keratinization.

Oral squamous cells carcinoma is one of Keywords: Haematoxylin and Eosin; Keratin; worldwide spreading tumours. Those types of Modified Papanicolaou; Oral squamous cells tumours characterized by the formation of carcinoma. keratin. This study aimed to compare between hematoxylin and eosin (H&E) stain, and modified Papanicolaou (PAP) stain with respect to the staining specificity and intensity of keratin P091 present in different cases of oral squamous cell Instance selection algorithms for a Cost carcinoma (SCC). Analytical cross-sectional study sensitive medical diagnosis conducted at the University of Medical Sciences and Technology (UMST) and University of Meryem Saidi1,2*, Nesma Settouti1, Mostafa El Habib Khartoum (U of K) on (n= 221) different cases of Daho1, Mohammed El Amine Bechar1. oral squamous cell carcinoma. Duplicated section were taken from each block, one stained by H&E Higher School of Management of Tlemcen. and the other by modified PAP stain. Stained Biomedical Engineering Laboratory GBM, Tlemcen sections were then evaluated based on the University, Algeria. specificity and intensity of staining keratin using [email protected] modified scoring criteria of poor, satisfactory, good and excellent. Statistical analysis for data Abstract were analysed using S.P.S.S (Statistical Package for the Social Science) software, version 21 using Classification is an important process in medical McNemar-Bowker Test and Wilcoxon Signed diagnosis. An early detection reduces Ranks Test. P-value < 0.05 were considered significantly the mortality risk. For that reason, statistically significant. The staining specificity of in medicine misclassifying an abnormal sample modified PAP stain were statically significant as a normal sample is highly expensive. However, with P value = 0.0000001, and staining intensity a great majority of traditional classification with P value = 0.00002 than H&E stain. Modified algorithms tend to minimize the classification PAP stain gives a better staining specificity and error without considering the misclassification intensity and easier diagnosis of keratinization in cost. On the other hand, medical datasets are cases of oral SCC than H&E stain, because it highly imbalanced since there are many more provides better contrast to the background, normal samples than abnormal ones. Therefore, allowing easy identification of keratin and more the abnormal cases are generally misclassified. In accurate diagnosis of the cancer. This results this paper, the authors compare the effectiveness were consistent with those of Ramulu et al. of using a resample process with instance (2013), but contrary to those of Rao et al. selection algorithms to reduce the because phloxine-B was used in the current misclassification cost. We tested these algorithms

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on several medical datasets from UCI machine differentiated hepatocarcinoma is the most learning repository with different imbalanced common (78%). The results of the ratio. The results show that evolutionary immunostaining carried out on the 40 algorithms outperform the traditional instance pathological cases show a correlation between selection approaches. the expression of β-catenin in the different cellular compartments and the degree of Keywords: Cost sensitive learning, instance differentiation as well as the severity of the cases selection algorithms, medical diagnosis. of cirrhosis and viral hepatitis. Male predominance of hepatocellular carcinoma in various national and international studies [5 and 6], consumption of alcoholic tobacco, and high P092 rate of liver disease in men were found to explain Interest of beta catenin in the diagnosis and this result. Immunohistochemistry results targeted therapy of hepatocellular cancer suggest activation of the WNT / β-catenin pathway in cases where immunolabeling is Lilya HOUARI 1*, Leila SAADI1,2, Wassim cytoplasmically and nuclear positive, correlating BOUHACEIN1, Hayet Aourague1, Abd ElKarim BAHA3. with various studies. These results allow us to suggest the direct involvement of β-catenin in 1Laboratoire de Biologie Moléculaire et Cellulaire, hepatocarcinogenesis, which favors the use of Département de Biologie et de Physiologie Cellulaire, Faculté this molecule in the diagnosis, monitoring and SNV, Université Blida 1. 2Laboratoire de recherche d’Ecologie Animale, ENS, Kouba, development of therapy. Alger. 3Service d’anatomie Pathologie, CHU de Béni Messous, Alger. Keywords: Hepatocellular carcinoma; B-catenin; [email protected] Cirrhosis; Hepatitis B.

Abstract P093 Hepatocellular carcinoma (HCC), the most common malignancy of the liver, is the sixth most Incidence and diagnosis of thyroid cancer in common cancer in the world, and the third eastern Algeria: a retrospective study of 339 leading cause of neoplasia. Occurring in nearly cases over 24 cities 80% of cirrhotic liver cases or in the context of chronic liver diseases including viral hepatitis B Latifa Khattabi 1,2*, Khaoula Boulkrout 1, Fériel Sellam and C. Hepatocarcinogenesis remains at present 2 a poorly known process. This work aims to study University of Brothers Mentouri Constantine I. the involvement of the β-catenin adhesion National Center of Biotechnology CRBt Constantine molecule, in the development of this pathology as [email protected] well as the possibility of its use as tools for early diagnosis, follow-up and as a therapeutic target. Abstract The work is based on the search for the The incidence of thyroid carcinoma TC has localization and the expression level of β-catenin alarmingly increased over the last couple of by immunohistochemistry, from paraffin blocks decades, globally as well as in Algeria. We of 40 pathological cases divided between undertook an epidemiological study consisting in hepatocellular carcinoma, metastases, cirrhosis the census of patients suffering from this type of and viral hepatitis, also a Retrospective study is pathology as a means to report new disclosures carried out on 300 cases collected over five years regarding its risk factors, causes, diagnosis and (2017-2013) including 70 cases of hepatocellular evolution. The study was carried out using data carcinoma and 230 metastases. The from the records of the nuclear medicine retrospective study shows a male predominance department of Benadis Constantine University for hepatocellular carcinoma with maximal Hospital; it covers 339 cases with TC. It refer to frequency in the elderly (over 70 years), as well the period between 2011- 2013, patients reside as a predominance for metastases of mammary in 24 different cities over the eastern region of origin (32%). The distribution according to the Algeria (north and south). The diagnosis was state of cell differentiation shows that well- based on anatomopathological and para-clinical

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reports (ultrasonography, blood test, P094 radiography, scintigraphy, scanner and MRI). The Monte Carlo simulation in mammographic population represents a remarkable female dominance with a percentage of 87.90% and a imaging and evaluation of the dose sex ratio of 0.13, this is almost related to sex distribution hormones and menopause. According to the 1 1 statistics the most affected age group is [40-50 [ Billel Abdelaziz Bouchikhi Saadia Benhalouche , Anis Ahmed Samy Dib1, Abdelghani Tebboune1, and the average age is 43.5 years old, Our results Ahmed Hafid Belbachir indicates that Setif is the most affected city with a frequency of 50 cases; it is mainly due to the cold 1Department of Immunology, University climate that increases the risk factor of TC. Laboratoire d’Analyse et d’Applications des Rayonnements Regarding risk factors; 67% of cases have no LAAR. Faculté de Physique. Université des Sciences et de la Technologie d’Oran (USTO) Mohamed Boudiaf medical history, although 33% have different [email protected] medical histories namely: 35.22 % high blood pressure, 26.13 % diabetes, 11.36 % liver Abstract troubles, 9.09 % genital involvement, 6.81 % The high efficiency of medical images is an renal impairment, 4.54 % Asthma, 4.54 % heart important factor in mammography because they disease, 1.13 % psychiatric disorders and 1.13 % allow a reliable diagnosis to detect lumps, rheumatism. TC development in 126 patients is whether clinical abnormalities or screening for associated with another thyroid pathology type, cancerous lesions such as breast cancer for 46 cases suffered from goiter, 27 had several women. Currently, only mammography shows nodules, 27 had Hashimoto's thyroiditis, 18 had capabilities to detect small lesions and with an hyperplasia, 1 case with Parathyroid Fat early diagnosis can reduce the mortality rate of Involution and 7 cases with Adenoma. Patients this disease. This is defined as a radiographic were diagnosed of papillary type as the most examination that uses X-rays to detect breast common class of TC: 291 cases, 40 cases tissue damage. Due to the advantages presented follicular, 7 cases poorly differentiated, 1 case by this imaging modality, we will be interested in medullar and no case has been taped for this thesis work on Monte Carlo simulation. anaplastic cancer. We noticed four subclasses of Monte Carlo codes designate a family of papillary CT with unequal proportions: classic algorithmic methods to compute an approximate papillary cancer 55.63% ˃ papillary-vesicular numerical value using stochastic processes, we 25.93% ˃ papillary micro-carcinoma 17.06% ˃ will rely for it on the Monte Carlo GATE (GEANT4 papillary-trabecular 1.36%. In eastern Algeria code Applied for Tomographic Emission), for the (North and South) TC is more frequent in women simulation of mammography dedicated device. It than in men. The development of TC is will involve the simulation of a complete moderately associated with some benign thyroid mammography setup, with a conventional X-ray diseases. Risk factors vary depending on the source and the several accessories used to shape presence of medical history and eventual the X-ray spectrum (anode, filters, detectors,) to environmental triggers. Setif as a city shows the reproduce a realistic mammography highest incidence of TC. The most abundant class examination. We will then proceed to calculate a of the TC is papillary carcinoma in its classical number of parameters such as the dose form and vesicular variant. distribution, the average glandular dose that can be validated by a comparative clinical study. Keywords: diagnosis; incidence; risk factors, thyroid cancer. Keywords: mammography, GATE Monte Carlo simulation, breast cancer.

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P095 carcinoma) and this by its expression on the tumor tissues but also by the presence of Descriptive and Immunohistochemical Study mutations which in the absence of tobacco of Lung Cancer consumption, induce in a strange way the phenomena of the carcinogenesis. Several studies Imene Bendoukha1*, Fatima Zohra El kebir2, Amal of the mutational status of EGFR and its Mesli2. involvement have resulted in the development of Antifungal Antibiotics Laboratory: Physicochemistry, targeted therapies based on tyrosine kinase Synthesis and Biological Activity. ABUBEKR BELKAID inhibitors, used for patients whose University of Tlemcen. chemotherapy is ineffective. Faculty of Sciences of Nature and Life, AHMED BEN BELLA University of Oran. [email protected] Keywords: Epidermal Growth Factor Receptor; Lung Cancer; Monoclonal Antibodies; Small Cell

Lung Carcinoma. Abstract

Despite significant progress in detection or P096 treatment, the prognosis for bronchial cancer remains bleak. Less than 15% of diagnosed Vibrational Spectroscopy – A diagnostic tool in patients survive their illness due to a late oncology diagnosis of the disease, which is already well advanced. Bronchial cancer evolves in a silent Alaa Mohamed Foued DERDOUR 1*, Hichem NAAS 2, way and the symptomatology of call is often Abdelaziz GHARBI 3. commonplace with a late effect. These last years, Resident Pharmacist in Analytical Chemistry, Department of the biological evidence for carcinogenesis has Pharmacy, Saad Dahleb University, Blida. evolved with the discovery of new biomarkers. Resident Pharmacist in Analytical Chemistry, Department of These are the witnesses of the cancerous disease Pharmacy, Saad Dahleb University, Blida. and have an interest in the improvement of the Industrial Pharmacist, Professor of Analytical Chemistry, diagnosis. Thus, the use of immunohistochemical Department of Pharmacy, Saad Dahleb University, Blida. [email protected] staining techniques makes it possible to improve the diagnosis establishment while specifying the histological type. The latter can be supplemented Abstract by molecular biology techniques that seem to be of greater benefit in the clinical diagnosis. The Spectroscopy is the study of the interaction of realization of the immunohistochemical staining different types of electromagnetic radiation with technique using monoclonal antibodies by matter. Indeed, molecules, or atoms, can absorb adopting the EnVison + kit method, allowed us to or emit electromagnetic radiation at distinct carry out the labeling of EGFR and CK5 / 6 on the frequencies. The set of all these radiations histological type pulmonary adenocarcinoma. constitutes a spectrum characteristic of the This allowed us to make a final and differential molecule, or the atom, in question. When a diagnosis with respect to the other histological molecule is exposed to certain electromagnetic types in addition to the histological techniques of radiations, such as Infrared radiation (IR), it Hematoxylin-Eosin staining. The results obtained changes its vibrational state. The study of the by carrying out the immunohistochemical resulting spectrum is vibrational technique using anti-EGFR and anti-CK5 / 6 spectroscopy. This branch of spectroscopy monoclonal antibodies (EnVision + method, includes the Infrared spectroscopy: which Dako), allow selective and additional diagnosis of studies the absorption spectra in the Infrared the different histological types of domain (0.78-1000 μm); and Raman bronchopulmonary cancers. spectroscopy: based on the Raman effect, or the Immunohistochemical analysis has significantly inelastic scattering of radiation by the molecules. improved the clinical diagnosis and management Both techniques provide information about the of the patient in selecting the type of treatment. A identity and the structure of molecules through strong implication of the EGF receptor has been the study of changes in their vibrational reported in the NSCLC (small cell lung state. Thereby, these techniques have great potential as medical diagnostic tools, particularly

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in oncology. In fact, vibrational spectroscopy total number of 82 patients.The proportion of allows the detection of molecular changes that PCa with a Gleason 7 score appears significantly occur in cells, tissues, and biofluids, which can be higher than that of a 6 score. Anatomopathology at the origin of the pathology, or one of its effects. plays a vital role in the diagnosis of CaP. Only the examination of biopsies affirms with certainty Keywords: Cancer, Diagnosis, Oncology, the diagnosis of CaP from the analysis of Vibrational Spectroscopy. histological sections. The results of this study show that the diagnosis is often made too late in advanced stage with a high PSA levels and biopsy P097 showing high levels of Gleason. Early detection of Value of Anatomopathological Examination in cancer through early diagnosis and population screening must be a priority to reduce the high Prostate Cancer incidence of late stage cancer cases, thus increasing survival rates, which remain very low, Yasmine Bekas1, Omar Djeffal2, Hajira Berredjem1 compared to Western countries 1University Badji Mokhtar-Annaba, Department of Biochemistry, Laboratory of Applied Microbiology and Key words: Prostate cancer; PSA; Biopsies; Biochemistry, Algeria Gleason score; Histology. 2Uro-Surgery Medical Cabinet, Annaba, Algeria [email protected]

P098 Abstract Contribution of BRCA1 and BRCA2 Germline Mutations to Early Algerian Breast Cancer Prostate cancer (PCa) is the most common cancer using High Resolution Melting Analysis (HRM) in men over 50 years and the 5th leading cause of cancer deaths in men, with about 1.1 million new Amina Chami1*, Farida Mesli 1, Florence Coulet2, cases diagnosed and 307000 deaths in 2012. Fatima Zohra Boudinar3. Rachid Senhadji1. There are two tests for PCa: a rectal exam and a Prostate Specific Antigen (PSA) test. These are Oran 1 university. Gh la pitie salpetriere. the best ways to detect PCa as early as possible, Centre anti cancer. [email protected] but currently they do not confirm the diagnosis. To determine whether a patient is suffering from Abstract PCa, a biopsy is performed for Several studies have shown that factors anatomopathological investigation. So, PCa implicated in breast carcinogenesis might have a histology, especially Gleason score, plays a different impact on the risk of developing breast critical role in predicting patient outcomes. This cancer in Algerian women. Tumours occurring at work aims to contribute to the study of the value an early age have a poor prognosis. The genetic of PCa anatomopathological test in a population mutations are the cause of occurrence of cancer of Eastern Algeria. This retrospective study at an early age even in the absence of a familial included 264 patients over a period of three context. Germline mutations in the BRCA1 and years from January 2015 to December 2018.The BRCA2 genes in patients with early-onset breast study was based on the informations collected on cancer have not been clearly identified within the the register patient’s. The age, the PSA and the west Algerian population. Our aim is to analyze histological grade features were taken in BRCA1 and BRCA2 genes mutations among 50 consideration for all patients. The highest consenting young women from western Algeria number of patients was observed in the age diagnosed or treated with primary invasive group 70-79 years. The average rate of total PSA breast cancer at the age of 40 or less who were was 28.51 ng/ml (ranged between 4-92 ng /ml). referred through the Military Teaching Hospital 53% of patients had a PSA  20 ng/ ml. Histology of Oran. Two deleterious mutations have been confirmed that all patients accounted for identified (C.2125- 2126 inSA) located on the prostatic adenocarcinoma with prognostic exon 11b of BRCA1, and (c.250c>T) located on Gleason score that ranged between 6 and 8.The the exon 3 of BRCA2 gene. Moreover five variants proportion of PCa with Gleason 6 and 7 was the of uncertain clinical significance and favor most frequent in the studied population with a

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polymorphisms were identified. Four rare in tetrapolar configuration based on four variants of an unknown biological significance electrodes. The interface based on LABVIEW was (UVs) have been identified on BRCA1 gene used for evaluation of resistance and reactance of (rs498650, rs1799949, rs16940, rs16942) and bioimpedance. This device has been on the BRCA2 gene (rs2889772). Our data characterized with RC networks and validated by suggest that BRCA1/2 mutations are responsible testing its current output over a range of for a significant proportion of breast cancer in frequencies between 1 kHz and 1 MHz and Algerian young women. comparing measured values of impedance across a test circuit to expected values. The measured Keywords: Breast cancer, BRCA1, BRCA2, value of impedance in a test circuit followed the Western Algeria impedances of RC networks according to the Fricke, Cole and Debye models. Bioimpedance is a well-established technique in detecting breast P099 cancer, cervical cancer, and prostate cancer. In The Electrical Bioimpedance Technique in this work bioimpedance spectroscopy is performed by two electronic stages and an Cancer Diagnosis interface based on LABVIEW. The performance of our device is tested by RC networks used Salim Kerai *, Hadjer Benabdallah asvirtualtissues. With this medical device, a lab Department of Biomedical Engineering, URMER, or research group can begin introductory study Faculty of Technology, University of Tlemcen, Algeria to confirm significant differences in [email protected] bioimpedance between normal tissues and Abstract cancerous tissue. Electrical bioimpedance technique has been Keywords: Bioimpedance; Cancer; Diagnosis; widely used in human physiology to estimate spectroscopy. hydration levels, water weights during pregnancy, fat levels, cardiac output and cancer diagnosis. P100 Several methods are used to measure electrical bioimpedance: bioelectrical impedance analysis Investigation of colorectal cancer via an (BIA), electrical impedance spectroscopy (EIS), anatomopathological approach impedance plethysmography (IPG), impedance cardiography (ICG) and electrical impedance Asmahane Medjdoub1,2*, Zineb Tahari1,2, Fatima tomography (EIT). The studies showed that there Zohra Tahari3,2,Tewfik Sahraoui 2. are significant differences in bioimpedance University of Science and Technology Oran Mohamed Boudiaf between normal tissues and cancerous tissue. In Laboratory of Development and Differentiation Biology, this work, we designed an electronic circuit University of Oran 1 Ahmed Ben Bella prototype to measure and analyze the electrical University Center Belhadj Bouchaib Ain Temouchent bioimpedance for normal and cancerous tissue. [email protected] Bioimpedance spectroscopy (BIS) of tissues has Abstract been used for identifying cancers by Colorectal cancer usually occurs after 50 years investigating their resistance and reactance in RF (95% of new cases, 46% after 74 years).The and correlating these to malignancies of a variety majority of colorectal cancers arise from benign of tissues. Our bioimpedance device is lesions such as polyps that develop on the accomplished in tree sections comprising of mucosa and can develop into cancer. Our work is current injection stage, voltage detection and based on an anatomopathological study of resistance and reactance evaluation interface. A colorectal cancers to determine the most Howland voltage controlled current source based common histological varieties. This study was on TL082 operational amplifier integrated circuit conducted at a hospital-university level, and was used to inject a current for measuring included eight cases of colorectal cancer bioimpedance. An AD620 instrumentation including 3 women and 5 men. The tissue amplifier was used for the voltage measurement. samples are obtained either by biopsy or by The bioimpedance measurements were realized resection of an operative part or organ. Then

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they were fixed in formalin and then treated concerns 25 cases collected between January and according to a well-defined protocol to be cut April 2018 in various health sectors. We included and stained with hemalun-eosin. The reading of all patients with primary benign or malignant the slides is done with a computer-linked optical tumors of colon, of rectum or the both. Samples microscope which allows a better exploration of are fixed at 10% formalin; paraffin embedded the tumor and a recording of the images with and stained with haematin-eosin. CRC frequency their data. The results obtained revealed a male is higher among males (60.7%), than females predominance of 62,5% with a sex ratio of 1,66. (39.3%). The male/female sex ratio was 1.54. The average age is 55 and dominance has been The mean age was 55.2 ± 16.3 years. Colorectal noted in the 50-60 age group, which is 37% of cancers predominate with 16 cases against 9 our population. Histological analyzes reveal that benign tumors. Malignant tumors were more 75% of colorectal cancers are adenocarcinoma common in the age group 50-59 years with lieberkühniens, and 25% undetermined. The (31.25%) and benign tumors are very present distribution according to the degree of among individuals in the age group 40-49 years differentiation of adenocarcinomas indicates that (33.33%).Whatever the nature of the tumor, the 62,5% are well differentiated, 12,5% moderately male sex was predominant with a sex ratio of 2 differentiated and 25% little or no differentiated. (6 cases against 3) for benign tumors, and 1.28 (9 The choice of treatment depends on the stage cases against 7) for malignant tumors. Our study and location of the cancer. It is never decided by shows that colorectal cancer is very often a single professional but is the result of a diagnosed at a late stage. Otherwise we multidisciplinary consultation meeting. Major recommend regular molecular screening tests to advances in understanding tumor biology, help prevent colorectal cancer by identifying and biomarker development, and targeted therapy monitoring benign tumors before they turn into have improved the treatment options for cancer. colorectal cancer. Keywords: Colorectal cancer; Histopathology; Keywords: Colorectal cancer; Lieberkühnien Diagnostic; Polyps. adenocarcinoma; Polyps; Risk factors.

P103 P102 Epidemiological and diagnostic aspects of 350 Epidemiological and histopathological aspects colorectal cancers cases in Sidi Bel Abbes of colorectal tumors in a West Algerian (Algeria) population Fatima Zohra Chenni1 *, Samira Meziani1, Amina Zineb Tahari 1,2*, Asmahane Medjdoub 1,2, Fatima Mattalah1, Ouahiba Ouldnnebia1 Zohra Tahari3,2, Houria Ouldcadi2, Tewfik Sahraoui 2 1 Department of Biology, BiotoxicologyLaboratory, SNV Faculty of Djilali Liabes of Sidi Bel Abbes University, BP University of Science and Technology Oran Mohamed Boudiaf 89,22000, Algeria Laboratory of Development and Differentiation Biology, [email protected] University of Oran 1 Ahmed Ben Bella University Center Belhadj Bouchaib Ain Temouchent [email protected] Abstract Abstract Colorectal cancer is a worldwide health problem. In Algeria, this type of cancer is widely frequent Colorectal cancer (CRC) is one of the most and remains a major public health problem. In common causes of cancer morbidity both in men fact, colorectal cancer is the second leading cause and in women. In more than 80% of cases, this of death among cancers that affect both women cancer comes from benign tumors like polyp. and man. The aim of this study is to highlight the There is growing evidence that polyps represent epidemiological and diagnostic aspects of a precursor lesion of CRC. Our purpose is to colorectal cancer in the cancer center of Sidi Bel explore benign and malignant colorectal tumors Abbes (West of Algeria). We conducted a by studying epidemiological and histological retrospective study of 350 adults who were profiles in West Algerian population. Our study

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diagnosed with colorectal cancer between On the occasion of this observation, we January 2010 and December 2017. The results of emphasize the importance of the this study show that the mean age at diagnosis immunochemical and the immunophenotypic was 57 years and the sex ratio was 1.22 with a analysis in the diagnosis and the monitoring of predominance of males. Common histological PCL. type was adenocarcinoma (74%). Most common Serum protein electrophoresis, immunofixation site of involvement is colon (72%), which is and free light chains (FLC) assays were followed by rectum (22%). Mean body index was performed for the patient A.B: 50-years old mal > 25 in 31 % of colorectal cancer patients. At diagnosed with PCL based on the % (≥20%) and diagnosis, 50 % of patients were in stage III. The absolute number (≥2x109 /L) of plasma cells in serum levels of CA 19-9 and carcinoembryonic the peripheral blood. The antigen (CEA) were determined in 60% of immunophenotyping has been performed by 8 patients and 53% of the CA19-9 level was colors flow cytometer. The serum proteins increased in only 5 % of patients. The CEA level electrophoresis reveals the presence of a was elevated in 18% of patients. Colon cancer is monoclonal paraprotein evaluated at 41 g/L more frequent than rectum in Sidi Bel migrating in the gamma region. Immunofixation Abbes.Colorectal cancer patients are diagnosed confirms that this is a monoclonal IgG Lambda at later stage. Carcinoembryonic antigen and intact immunoglobulin. FLC ratio was abnormal (CEA) and carbohydrate antigen 19.9 have yet to (0.02 < 0.26). These results confirm the secretory be used as prognostic factor, but not for all characteristics of this PCL. These secretory patients, for colorectal cancer. plasma cells are CD38+ CD138- CD27- and CD19- . The mechanism of extramedullary spread of Keywords: Colorectal Cancer; Retrospective malignant plasma cell is explained in the Study; CEA; CA 16.9.Adenocarcinoma; Sidi Bel literature by the lack of CD56 (NCAM) which Abbes does not agree with our case where the CD56 was positive. The immunophenotypic results confirmed the diagnosis and can be used for the P104 evaluation of minimal residual disease however Primary Plasma Cell Leukemia: the follow-up of the level of the monoclonal immunoglobulin and the FLC should be used as Immunochemical and Immunophenotypic response criteria. Features Keywords: Plasma Cell Leukemia; Heyet Ramdani1*, Mohamed Brahimi2, Youcef Bouali- Immunophenotype; Serum Protein Youcef1 Electrophoresis; Immunofixation; Free Light Department of Immunology, University Hospital Chains. Establishment 1st Nov, Oran, Algeria Department of Hematology, University Hospital Establishment 1st Nov, Oran, Algeria [email protected]

Abstract

Plasma cell leukemia (PCL) is a rare and aggressive variant of myeloma characterized by the presence of circulating plasma cells. PCL can either originate de novo (primary PCL) or as a secondary leukemic transformation of multiple myeloma (secondary PCL). The prognosis of PCL is poor with a median survival of 7 to 11 months. The International Myeloma Working Group (IMWG) recommends that the evaluation of response in primary PCL should combine acute leukemia and multiple myeloma requirements.

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P105 to note that overexpression of EGFR, induced by hyperactivation of its promoter region, has been Distribution study of G216T polymorphism of described in several cancerous pathologies and is the EGFR gene and evaluation of its often associated with poor prognosis, as reported therapeutic prognosis in Non-Small Cell Lung in this recent study on the Algerian population. Carcinoma. Keywords: NSCLC; SCLC; EGFR; Squamous 1* Amal Mesli , Katia Solchurch 2, Deborah Stabley 2, carcinoma; Sp1; G216T; C191A. Ahmed Kacimi 3, Nacera Belblidia 3, Bahia Merad 4, Fatima Zohra El Kebir 1, Tewfik Sahraoui 1.

Biology of Development and Differentiation Laboratory, P106 University of Oran 1 Ahmed Benbella, Algeria. Nemours Biomolecular Core Laboratory, Alfred I Du Pont Oral intake of aqueous extract of Graviola Hospital for Children, Wilmington, DE, USA. (Annoneceas Muricata) dried leaves Pathological Anatomy Service, Mohammed Seghir Nekkache Hospital, Algiers, Algeria. suppresses the 7,12-DMBA/Croton oil Laboratory of Anatomy and Cytopathology, Oran, Algeria. induced two-step skin carcinogenesis in Swiss [email protected] albino mice

Mohammed Abdellaoui1*, Assia Kadi1, Omniya Abstract Benotmane1, Amina Zahra Benhadda1, Mahfoud Despite numerous therapeutic advances, lung Messarah 1. carcinomas prove to be indestructible because of 1Laboratory of Biochemistry and Environmental Toxicology, their aggressiveness and poor prognosis, thus Universite Badji Mokhtar -Annaba- ALGERIA. causing the most cancer deaths in the world. [email protected] Patients who present themselves often at an advanced stage have their chances of survival minimized and especially shortened. This study Abstract was performed on 20 patients with non-small The phytochemical complexity of plant extracts cell NSCLC and small cell lung carcinoma SCLC to may have chemotherapeutic and establish a differential diagnosis. The search for chemopreventive effects. The purpose of this possible genetic polymorphisms located in the study was to assess the potential of extracts from region of the promoter and part of exon 1 of the the tropical tree Annona muricata (Graviola) as a EGFR gene was performed by sequencing this therapeutic and preventive agent of cancer. Its gene region using a ABI 3100 capillary composition is rich in Annonaceae acetogenins electrophoresis. Sequencing of the promoter which are strong nicotinamide adenine region and part of exon 1 of the EGFR gene dinucleotide oxidase inhibitors of the cancer revealed the presence of a G216T polymorphism cell's mitochondrial membrane. We divided 20 in the Sp1 transcription factor binding site in mice from the Swiss Albinos strain into four 64.70% of NSCLC patients, accompanied by a groups: 1/witnesses, 2/carcinogenicity using clear predominance in squamous carcinoma. This DMBA as the initiating agent and Croton oil as variation induces the change of a guanine to the promoter, 3/ receiving treatment with thymine (-216G /T) and thus makes it possible to Graviola leaf extract alone, 4/ receiving GLE in increase its transcriptional activity by 30%. parallel with carcinogenesis. The tests on animals Another nucleotid variation C191A was also lasted 19 weeks, and were followed by an found in the promoter region inducing the anatomopathology that tends to evaluate the change from cytosine to adenine. It is located development of pre-cancerous lesions as well as upstream of many transcription initiator sites inflammatory skin conditions. Subsequently, we that can exert a regulatory role on the EGFR measured the enzyme activity of SOD, CAT and promoter. The distribution analysis of the four GPx allowing the study of oxidative stress haplotypes (G-C, G-A, T-C and T-A) and the parameters. The results revealed the diplotype (G-C / T-C) of the G216T and C191A development of precancerous hyperplasia polymorphisms was performed to analyze affecting the epidermoid (spinous) cells in the possible functional correlations. It is important second group. In contrast, no hyperplasia was

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observed in the mice of the other three groups by conformity with the Preferred Reporting Items submitting that the GLE in the mice of group 4 for Systematic Reviews Meta-Analyses (PRISMA) interfered with the development of the DMBA- statement. Phase III randomized clinical trials induced tumour. Carcinogenesis causes were identified through a systematic literature disruption in the enzyme activity of antioxidant search over PubMed, Cochrane, and the enzymes: SOD, CAT and GPx. The cancer pathway clinicaltrials.gov databases up until August 15th, appears to decrease the activity of SOD while the 2019. The included studies provided hazard healing pathway increases it. Regarding the ratios (HRs) for overall survival (OS), immune system we found no significant effect of progression-free survival (PFS), and odds ratios the extract on the activation of the immune for objective response rates (ORR). The generic system and the level of immune cells remains inverse variance method was used for the pooled almost unchanged and this result is opposed to analysis. The statistical analyses were carried out certain studies, especially that done on using the R statistical program (version 3.6). Desacetyluvaricin, which shows an activation of Thirteen phase III randomized controlled trials the Innate immunity. The aqueous extract of the were included involving 7 371 cancer patients. leaves of Graviola improves the antioxidant The trials examined the efficacy of Nivolumab in activity of the organism on the other hand it patients with melanoma, non-small cell lung would not boost the immune system, soon, we cancer (NSCLC), renal cell carcinoma (RCC), plan to study the effect of the plant on the third Squamous Cell Carcinoma of the Head and Neck and fourth stage of skin cancer, namely, (SCCHN), and gastric cancer (GC) [16] with PD- carcinoma and melanoma. L1 tumor expression levels < 1%, ≥ 1%, < 5%, ≥ 5%, < 10%, and ≥ 10%. The HRs for overall Keywords: Graviola; acetogenins; survival, among patients with PD-L1 < 1%, were Dimethylbenzoantracen (DMBA); Oxidative (0.71; 95% CI 0.58 ;0.87) for melanoma, (0.77; stress; Papiloma 95% CI 0.61; 0.97) for NSCLC, (0.89; 95% CI 0.54; 1.45) for SCCHN, (0.98; 95% CI 0.56; 1.72) for RCC, and (0.76; 95% CI 0.49; 1.18) for GC. Among patients with PD-L1 ≥ 10%, the pooled HRs were P107 (0.65; 95% CI 0.46; 0.92) for melanoma, (0.43; 95% CI 0.31; 0.60) for NSCLC, and (0.56; 95% CI Tumor PD-L1 expression and Nivolumab 0.31; 1.01) for SCCHN. Similar pattern was efficacy in the treatment of cancer: a meta- observed for PFS. The summary of HRs for the analysis of phase III randomized clinical trials PD-L1 <1% subgroup were (0.54; 95% CI 0.40; 0.73) for melanoma, (0.75; 95% CI 0.44; 1.29) for 1* Salah Eddine El Herrag , Méghit Boumediène NSCLC, (0.39; 95% CI 0.09; 1.67) for SCCHN, and Khaled 1,2. (1.00; 95% CI 0.80; 1.26) for RCC. Among Djillali Liabes University, Faculty of Life and Natural Sciences, patients with PD-L1 ≥ 10%, the pooled HRs were Department of Biology. PO Box 89, Sidi-bel-Abbes (22000), (0.43; 95% CI 0.28; 0.65) for melanoma, (0.54; Algeria. 95% CI 0.40; 0.73) for NSCLC. According to our Laboratory of Health & Environment, Djillali Liabes findings, patients with higher tumor PD-L1 levels University, PO Box 89, Sidi-bel-Abbes (22000), Algeria. * [email protected] have benefited the most from treatment with Nivolumab, where the overall survival and progression-free survival were longer for this Abstract subgroup compared with patients with lower PD- L1 levels. We, therefore, suggest that tumor PD- Nivolumab is a fully-humanized IgG4 monoclonal L1 expression may be taken into consideration antibody that binds to the PD-1 receptor when assigning Nivolumab for the treatment of expressed by activated T-cells blocking thus the the investigated cancers in our study. immune checkpoint and activating an immune response to tumor cells. We conducted a meta- Keywords: Neoplasms; Nivolumab; PD-L1; meta- analysis with the aim of investigating the effect of analysis. tumor PD-L1 expression on the efficacy of

Nivolumab in the treatment of different types of cancer. The meta-analysis was performed in

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P108 in the metastatic process and it different roles in cell adhesion. The results are in favor with the Study of the possibility of using use of the molecule in the differential diagnosis, carcinoembryonic antigen 5 in the diagnosis followed and therapeutic development of of liver metastases and for hepatocellular cancers. It would be interesting to deepen this cancer research by the techniques of molecular biology.

Wassim Aymen Djihad Bouhaceine1*, Leila Key words: Carcino-embryonic antigen 5; Liver; Saadi1, 2, Lilya Houari1, Hayet Aouragh1, Abd El Immunohistochemistry; Metastases. Karim Baha3, Nadia Ait Kassi4, Nadia Benkhada4

1Laboratoire de Biologie Moléculaire et Cellulaire, Département de Biologie et de Physiologie Cellulaire, Faculté P109 SNV, Université Blida 1. 2Laboratoire de recherche d’Ecologie Animale, ENS, Kouba, Occurrence of oral and oropharyngeal Alger. 3Service d’Anatomie Pathologie, CHU de Béni Messous, Alger. squamous cell carcinoma among patients in 4Service d’Anatomie Pathologie, Centre Mari Curie, CHU Basrah city Mustapha Bacha, Alger. * [email protected] ; [email protected] Maha M. Al-mahfoud 1, Ihsan E. AlSaimary 1 and Ali. A. Al shawi 2

Abstract Department of Microbiology/Collage of Medicine/ University of Basrah / Basrah. It was found that several liver metastatic cancers Collage of dentistry / University of Basrah / Basrah. overexpressed the embryonic carcino antigen 5 * [email protected] (CEACAM5) which has been described for the first time as an oncofetal gastrointestinal antigen. This molecule is found to be overexpressed in the Abstract majority of carcinomas including those of the Oral cancer consider as the sixth common gastrointestinal tract, respiratory and malignant disease (its incidence is about third in genitourinary systems and breast cancer. While developing countries and eighth in developed other studies show the influences of CEACAM5 in countries). Squamous cell carcinoma (OSCC) general liver metastatic process by either account 95% of all oral malignant lesions. A few protecting circulating cancer cells from anokosis- studies have been written in Iraq regarding the induced cell death and by binding to incidence of oral cancer. The aim of this study heterogeneous nuclear RNA M4 which is a was to identify differences in oral and receptor for Kupffer. But there were no oropharyngeal squamous cell carcinoma patients conclusive studies showing its localsation in the according to age groups, gender, and location of liver itself in metastatic cases. The purpose of the tumorin Basrah city. This study included 21 this study is to find the marking patients with oral and oropharyngeal squamous carcinoembryonic antigen 5 in liver tissue with cell carcinoma were clinically diagnosed and cirrhosis, hepatitis, hepatocellular cancer and then confirmed by histopathological secondary metastatic foci of various origins. 230 examination. Age, gender, as well as clinical signs patients of different ages were the subject of a and the site of lesions, were recorded. There prospective study based on the were 21 new cases of oral and oropharyngeal immunohistochemistry technique performed on squamous cell carcinoma in Basrah from histological sections using primary and September 2015 to December 2016, 13 in men secondary antibodies. Analysis of results showed and 8 in women. Cancer at all oral sites affected a marked carcinoembryonic antigen only in the men more than women. The Tongue is the most metastatic cells 32% of the cases were breast frequent site. metastases origin. These results are similar to those found by Lee et al. While they are in Keywords: SCC, oral, Oropharyngeal squamous contradiction with several works that describe cell carcinoma the prevalence of colorectal metastases. These results confirm the involvement of the CEACAM5

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P111 Keywords: Immunohistochimical ; Immunohistochemical markers; Northeastern Immunohistochemical markers of mammary Algeria ; mammary tumors. tumors in female dogs from Northeastern Algeria

Zahra GABLI1, Abdelhafid GABLI2 P112

1Département des Sciences de la Nature de la Vie, Faculté des Interleukin-17A and NOS2 signaling Sciences, Université 20 Août 1955 Skikda, Algérie independently link NF-κB activity to epithelial 2Institut des Sciences Vétérinaires, Université des Frères Mentouri-Constantine 1, Constantine, Algérie to mesenchymal transition in invasive *Corresponding author e-mail: [email protected] laryngeal squamous cell carcinoma

Nora Kariche1, Nabila Moulaï2, Samir Benyahia3, Abstract Wahiba Ouahioune2, Djamel Djennaoui3, Chafia Touil- Boukoffa1, Mehdi Bourouba1* Research in the field of canine tumors has evolved and become more relevant in recent 1Department of Cell and Molecular Biology, Team Cytokines years; not only because the frequency of canine and Nitric oxide synthases. Faculty of Biology, University tumors is constantly increasing in veterinary Houari Boumediene USTHB, Algiers-Algeria 2Central Laboratory for Anatomopathology, Frantz Fanon medicine, but also because the mammary tumors Hospital, Blida-Algeria of the canine species is a good model for breast 3Oto-Rhyno-laryngology Department, Mustapha Pacha cancer in women. Mammary gland tumors are Hospital, Algiers-Algeria the most common tumors in the female dog. The * [email protected] present study was undertaken to investigate the importance of immunohistochemical markers (cytokeratins AE1/AE2, CK20, CK5, 6, RE, Abstract vimentin and P63) in tumor type diagnosis in the Laryngeal squamous cell carcinoma (LSSC) is an case of canine mammary tumors. Thirteen (13) inflammatory tumor of the head and neck tumors tissue specimens were obtained from 42 characterized with aggressive invasive behaviour female dogs in different ages and breeds. They and metastasis. Here, we aimed to address the had been classified according to WHO method possible contribution of nitric oxide synthase after histopathological examination. The tumors type 2 (NOS2) and IL-17A, two controversial were diagnosed as Squamous cell carcinoma (3 contributors of Epithelial to mesenchymal cases), Spindle cell carcinomas (2 cases), transition (EMT), to LSCC invasive features, Sarcomas (3 cases), Carcinoma simple cribriform together with NF-κB. To this aim, expression of (2 cases) and the epithelial component is NOS2, IL-17A and EMT markers (E-cadherin, β- malignant, and the myoepithelium is benign (3 catenin, vimentin and MMP-9) were assessed by cases). Cytokeratins AE1/AE2 were seen in all immuno-histochemistry in tumor biopsies cases except in the spindle cell carcinoma. obtained from 20 patients with invasive LSCC Vimentin can be used as a myoepithelial and and compared to NF-kB nuclear status. Spearman mesenchymal cell marker in all cases except in analysis of IHC scores showed that NOS2 the malin myoepithelioma, P63 is a sensitive and upregulation strongly associated with loss of E- specific myoepithelial marker in canine cadherin and gain of vimentin. We found mammary tumors. Whereas CK 5, 6 and RE were presence of a significant link associating specifically detected in simple cribriform acquisition of nuclear NF-κB labeling to loss of E- Carcinoma, cytokeratins CK20 were expressed in cadherin expression. We observed that as IL-17A all cases except in the Squamous cell carcinoma. labelling was enhanced in the tumor and the These results emphasize the interest of stromal areas of LSCC, a significant association immunohistochemical markers to identify the linked stromal IL17-A signal to nuclear NF-κB humoral cell origin in canine mammary tumors, staining, and E-cadherin loss. Multiple allowed distinguishing between canine correspondence analysis (MCA), showed that, mammary tumors of myoepithelial and lumino- acquisition of a mesenchymal profile was linked epithelial cells lineage in female dogs. to enhanced NOS2 and IL17-A stainings and nuclear NF-κB signal. To our surprise, no 108 Oran 05-07 December

significant correlations were found between genetic engineering and cell culture: all begins either tumor or stromal IL17-A stainings and with the creation of transgenic mice, into which NOS2 labelling. Altogether, our results suggest human genetic sequences, coupled with that in invasive laryngeal squamous cell lymphoid myelomas, have been inserted; «the carcinoma, IL17-A and NOS2 would hybridoma » obtained can be reproduced independently mobilize NF-kB nuclear activity to infinitely in a suitable sterile liquid culture support epithelial to mesenchymal transition to medium; a nutrient medium is then added which mediate tumor cell metastasis. will make it possible to obtain IgG1 anti-EGFR, which will subsequently be extracted from the Keywords: IL-17; NF-κB; NOS2; TEM. culture medium by centrifugation, purification and concentration, then put into a galenic form, and finally packaged in appropriate vials. MAbs P113 are innovative drugs that bring hope for effective cancer treatment; indeed, the observations made Formulation of biomedicines for the in the thousands of patients with metastatic CRC treatment of metastatic colorectal cancers: already treated, clearly demonstrate their clinical what is the role of the galenic pharmacist? interest. In the coming years, further progress will be needed to select relevant mAb targets, Allal Yasmin1*, Moussaoui Nassima2 reduce their immunogenicity, and reduce their cost. 1 Galénic Galénic laboratory, university of medicine, INESM, Oran, Algeria. Laboratory, university of medicine, INESM, Oran, Algeria Key words: Anti-EGFR monoclonal antibodies - * [email protected] Galenic development- Metastatic colorectal cancer. Abstract

Colorectal cancer (CRC) is one of the most common cancers in Algeria with 6500 cases P114 detected each year. More and more effective treatments to get rid of this disease are now Thymoquinone enhances IFN-γ-associated available. Indeed, thanks to medical research and antitumor cytokine production galenic development, monoclonal antibodies (MAbs) anti-EGFR, which have the ability to Miliani Maroua 1, Nouar Mouna 1, Paris Océane 2, 3 2 identify and block specific mechanisms of cancer Lefranc Gerard , Mennechet Franck , Mourad Aribi 1* cells, could be manufactured and put into dosage forms such as parenteral preparations. The aim 1 Laboratory of Applied Molecular Biology and Immunology, of this work is to define the place of galenic and Department of Biology, University of Tlemcen, 13000 pharmacy in the development of biomedicines. Tlemcen, Algeria. The goal is to describe the main manufacturing 2 Institut de Génétique Moléculaire de Montpellier (IGMM)– UMR5535, CNRS et Université´ de Montpellier, Montpellier, steps and controls of MAb-based drugs for the France. treatment of metastatic RCCs. This is a review of 3 Institut de Génétique Humaine, UMR 9002 CNRS-Université´ the scientific literature that has been developed de Montpellier, Montpellier, France. after consulting databases and search engines, mourad.aribi@ univ-tlemcen.dz especially « Pubmed, Google Scholar, et MedlinePlus ». Monoclonal antibodies (MAb) have profoundly modified therapeutics, Abstract especially metastatic CRC. The first MAbs were Interferon gamma (IFN-γ) is mainly produced by murine, but they soon proved to be ineffective cytotoxic T-cells and T helper type 1 (Th1) cells, and highly immunogenic. Biotechnology has and remains as the most important cytokine with made possible to obtain antibodies that are more antitumor proprieties, which act as proapoptotic human, that is to say chimeric, humanized and molecule. It has been used in clinical then completely human. Unlike chemical management of different type of cancers, synthetic drugs, MAbs are very complex including adult T-cell leukemia. In our study, we molecules, so their manufacturing process uses set out to evaluate the potential adjuvant effects

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of thymoquinone on the production of IFN-γ P115 content in the co-culture system of classically activated macrophages (Mϕ), pulsed or not with Role of Immuno-Oncology in Treating Cancer necrotic tumor cell lysates (NecrJCLs) with autologous CD4+ T-cells. Assay was carried out in Fizazi Anissa1,*, Bendahmane Malika2 co-culture system in the presence or absence of 1Departement of Biology , Faculty of Science of Nature and TQ at the dose of 5 μM. IFN-γ was measured in Life, University of Ahmed Ben Bella,Oran 1 , University Pole co-culture system after 24 h by a quantitative of Belgaid Mohamed BENAHMED, Bir El Djir, Oran (3100), sandwich enzyme-linked immunosorbent assay, Algeria using human ELISA kit of Sigma Aldrich Co., St. 2Laboratory of Research on Environment and Health (LRES), Faculty of Medicine, Hospital University of Sidi Bel Abbes Louis, USA. Absorbance measurements were (22000), Algeria. made at 450 nm with a microplate reader *[email protected] (Biochrom Anthos 2020, Cambridge, UK). TQ treatment of NecrJCL-pulsed Mϕ co-cultures resulted in a significant upregulation in the Abstract production levels of IFN-γ compared with For decades scientists around the world have untreated Mϕs co-culture. Furthermore, our been studying the role of the immune system in results corroborate a well-documented study the fight against cancer. However, it is only showing that TQ treatment in ovalbumin- recently that the long-term benefits of immuno- sensitized mice to air way inflammation resulted oncological treatments have been observed in in shifting the immune response from humoral patients who previously had few therapeutic toward cellular immunity as evidenced by an options. These treatments represent a "real increase in the production of Th1-associated breakthrough" in cancer therapy and could cytokine IFN-γ. Thymoquinone would have a revolutionize the way we treat many forms of powerful therapeutic effect on NecrJCL-pulsed cancer. The aim of this study is to understand Mϕ given its capacity to enhance the IFN-γ these new treatments, how they differ from production. Upregulation of IFN-γ-associated existing treatments and what role they will play antitumor cytokine production open up new in the years to come. Immuno-oncological possibilities in clinical settings for the treatment treatments use the body's natural immune of cancer using NecrJCL-pulsed Mϕs combined system to fight cancer. Immuno-oncological with TQ as immunotherapeutic adjuvant in T- treatments differ from existing treatments ALLs. because they target the immune system of the body, not the tumor itself; they allow the immune Keywords: Thymoquinone, Classically activated system to selectively recognize and attack cancer macrophage, Necrotic tumor cell lysate-pulsed cells; Give the immune system long-term macrophage, IFN-γ. memory, allowing it to adapt to cancer over time and to ensure a long-term, sustainable response to cancer. Immuno-oncological treatments should represent a significant part of anticancer therapy in the next few years, along with surgery, radiotherapy, chemotherapy and "targeted" therapies via antibodies directed against targets expressed by tumor cells (monoclonal antibodies).

Keywords: Immuno-oncology; Cancer; Treatments.

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Biosciences

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P116

Biochemical characterization and gene or together. Bacteriocins characterized during screening of bacteriocins produced by this study are interesting for food technology Enterococcus strains isolated from dried figs applications as potential biopreservatives. fermented in olive oil Strains can be used directly as starters of fermentation, in this case the GRAS status should Mohamed Merzoug 1,2*, Sabrina Amara2, Norel be checked. Pure bacteriocins can also be used Houda Bellaloui3, Belboula Fatima3, Noureddine but innocuity should first be checked in order to Karam2. be approved.

1 École Supérieure en Sciences Biologiques d’Oran (ESSBO), Keywords: Enterococcus; Bacteriocins; Class III; Oran, Algérie. Enterocins; Vancomycin. 2 Laboratoire de Biologie des Microorganismes et Biotechnologie, Université d’Oran1 Ahmad Ben Bella, 31000, Oran, Algérie. 3 Département des Sciences Agronomiques et Biotechnologie, Faculté des Sciences de la Nature et de la Vie, Université P117 Hassiba Benbouali de Chlef, Algérie. [email protected] Toxic hazard associated with vaccination

Chebli akli islam 1,*, Reffai Mohamed amine 1,, Abstract Zebbiche Younes 1,2, Bettane Kahina 1,.Hadjadja aoul1,2. Alamir.B1,2 Lactic acid bacteria can eliminate undesirable bacteria and preserve food from contamination, 1 Centre National de Toxicologie,NIPA delybrahim especially by producing bacteriocins. Our work 2 Facultés de médecine département de pharmacie Alger focused on the study of the bacteriocinogenic [email protected] activity of twelve lactic acid bacteria isolated from the traditional fermented dried figs with Abstract olive oil of Oran, and also on physicochemical and genetic characterization of their bacteriocins. Vaccination is the administration of an antigenic Eight strains have inhibited the indicator strain agent (the vaccine) to stimulate the immune HFM9, three of which were selected for the system of a living organism to develop adaptive physicochemical characterization of their immunity against an infectious agent. The active inhibitory substances. They have first been substance of a vaccine is an antigen whose identified with API-20E test strip and then tested pathogenicity of the carrier is attenuated in order for there antimicrobial activities by using the to stimulate the body's natural defenses (the double layer method as well as agar well immune system). The vaccine comprises: an diffusion method. The inhibitory substances have antigenic agent, adjuvants for increasing the been identified through physicochemical tests amplitude and durability of the vaccine response, and by using PCR-based screening. All strains and additives used as stabilizers and belong to the genus Enterococcus. The three preservatives. Among the most used adjuvants; bacteriocins studied have shown a high tolerance the aluminum salts, saponin, calcium phosphate, to pH variation (pH2-pH12) as well as to the the preservatives used are formaldehyde and different chemicals (SDS, β-mercaptoethanol and thiomersal, however the vaccines can contain EDTA), however, they have shown totally contaminants in this case heavy metals sensitives to proteases (pepsin, trypsin, protease (cadmium, mercury, lead ...). The risks associated S and α-chymotrypsin) and to high temperatures with aluminum are the immunoallergic effects, (80°C). They are, thus, heatlabiles and belong the macrophage myofasciitis and the neurotoxic probably to Class III of bacteriocins. Screening of effects (Alzheimer, ALS, Parkinson...), the enterocin genes as well as vancomycin gene by mercury meanwhile gives rise to allergic effects, PCR from total DNA of the eight inhibitory strains neurological and renal disorders. These inorganic indicated the absence of entP, ent31, cyl and vanA vaccine contaminants are controlled by genes and the presence of the two genes entA and instrumental methods such as atomic absorption entB in all inhibitory strains, either as single gene spectroscopy, and ICP coupled to a mass

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detector. The work that has been developed at the Iron III present in the complex K3Fe(CN) the National Center for Toxicology is concerned (FRAP method) and the third technique, consists with the determination of thiomersal, mercury, of the evaluation of the total antioxidant capacity and aluminum in a framework of toxicological CAT. The extract studied shows an important expertise, all the results obtained are in line with capacity to reduce the absorbance at 515 nm by the standards. Vaccination allows each year to reducing the concentration of DPPH radical in save millions of people, however, the vaccine- solution with an IC 50at 50µg/ml, However, the related toxic risk exists. Among the risk concentration effective against the reducing management approaches is the substitution of power was 700 µg/ml, for the total antioxidant aluminum adjuvants by other activity was 160mg EAG g-1. This important immunostimulatory molecules, strict control of activity was attributed to the phenolic contents vaccines, and alarming the immune system. due to their redox properties,which allowed toxicovigilance if a risk occurs in the population them to act as reducing agents. The results show an antioxidant efficacy of the polyphenol extract Keywords: vaccine, adjuvants, additives, toxicity, isolated from Juglans regia. It would therefore be vaccine controls. interesting to consider as a perspective to deepen the search for optimization of the extraction processes, and a possible screening of the antitumor activity in vivo and in vitro P118 Keywords: Juglans regia; polyphenol extract; Antioxidant profile of the polyphenol extract antioxidant activity; extraction by infusion. prepared by infusion of Juglans regia bark

Benahmed Nor-El-Houda1,2*, Azzi Rachid 2, Mouderas Faiza 2, Terki Mohammed P119 2.Benabderrahmane Mokhtar1. Research about the anioxydant activity of 1 DjilaliLiabes university of Sidi Bel Abbes, 22000 Sidi Bel Abbes, Algeria Citrullus colocynthis chloroformic exract 2 Laboratory : Antibiotics, Antifungals, Physico-chemistry, Synthesis and Biological activities, Department of Biology, Terki Mohamed*, Benariba Nabila, Benahmed Nour SNV-STU Faculty, University of Tlemcen, B.P.119, 13000 el houda Tlemcen, Algeria [email protected] 1 Antibiotique.antifungual laboratory: physico_chemistry , synthesis and biological activity. [email protected] Abstract There is much epidemiological evidence that Abstract treatment with medicinal plant could lower the risk of certain cancers. The effect has been This study was carried out to identify and attributed, in part, to natural polyphenols. quantify the total polyphenols and flavonoids Besides, numerous studies have demonstrated content in the chloroformic extract of Citrullus that natural polyphenols could be used for the colocynthis fruit. In the other hand, the evaluation prevention and treatment of cancer. Potential of its antioxydant activity using the antiradical mechanisms included antioxidant as well as the test on DPPH and the ferric reducing power modulation of multiple molecular events FRAP. The free radical scavenging effect of involved in carcinogenesis. The current results Citrullus colocynthis was determined according to summarized the antioxydant efficiency of major the method described by EL Haci et al and The polyphenol classes extracted from Juglans regia reducing power of Citrullus colocynthis extract ‘s barks. An extraction by infusion was prepared was assessed according to the method of from the barks of Juglans regia, the extract Karagözler et al. The quantity of total obtained underwent an evaluation of their polyphenols and flavonoids in the extract made antioxidant activity according to three by maceration was 117.27± 5.68 µg EGA/mg E techniques: one consists in trapping the free and 42.4±1.44 µg EC/mg E. The results of radical DPPH, a second consists in reduction of antioxydant power revealed a remarkable

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antiradical effect on DPPH with an IC50 of 274.28 increase in proteins content can be explained by µg/ml and the ferric reducing power was the synthesis of proteins involved in intracellular expressed by the value of EC50 of 267.96 µg/ml. sequestration of heavy metals like phytochelatin, The literature indicates that medicinal plants the activation of antioxidant enzymes may be due with high amounts of flanvonoids and phenol to the increase in the concentration of their have potent antioxidant activity actions. This substrates such as H2O2. This activation is may explain the antioxidant activity of Citrullus involved in the process of detoxification. Lead colocynthi. The results obtained during this study induces oxidative stress at the origin of the are promising, they inform us about the production of H2O2. Nevertheless, the plant antioxydant activities of C. colocynthis fruits by resorted to the use of antioxidant systems and confirming with a certain degree their traditional therefore, the activation of enzymes such as use. These results remain preliminary and they catalase and peroxidase, the understanding of the deserve to be thorough in order to identify and defense processes involved, could be at the origin characterize the compounds responsible for of discovery of substances with ecological and these activities and to try to know their pharmacological outlets in phytoremediation and mechanisms of action for their incorporation in phytotherapy. the care system. Keywords: antioxidant enzymes, Hordeum Keywords: Citrullus colocynthis; polyphenols; vulgare L, lead, oxidative stress. DPPH; FRAP.

P121 P120 Improvement of antivenom immunotherapy Antioxidative response to lead in Hordeum by Alum nanoparticulate adjuvant vulgareL Ghania Zidi1* , Faez Amokrane Nait Mohamed 2, Hafida Kazouz*, Miloud Slimani, Omar Kharoubi, Abdelmounaim Nouri 2 Nadia Ait Hamadouche, Abdelkader Aoues 1 Laboratoire de Microbiologie Appliquée, Faculté des Laboratory of Experimental Biotoxicology, Biodepollution and Sciences de la Nature et de la Vie de l’Université de Bejaia, Phytoremediation, Departement of Biology, Faculty of 06000 Bejaia, Algeria. Sciences of Nature and Life, University Oran 1 Ahmed Ben 2 USTHB, Faculty of Biological Sciences, Laboratory of Cellular Bella, B.P.1524, El M’naouer, 31000 Oran, Algeria and Molecular Biology, Bab- Ezzouar, Algiers, Algeria. [email protected] [email protected]

Abstract Abstract Among heavy metals lead is considered as a Snake envenomation is a major public health major pollutant. Once absorbed by plants heavy problem. Many symptomatic treatments are metals can generate an oxidative stress.The aim administrated, but Immunotherapy is the only of this work is to determine the effects of lead at effective treatment. Immunizing was producing different concentrations (100 mg/l, 500 mg/l and by animals needs strongly immunogenic 900 mg/l) on barley Hordeum vulgare L. After 30 formulations without being toxic to animals. days of growth, the plants were harvested; the Alum adjuvant is the most suited to the task due evaluation of oxidative stress was carried out on to its high-security profile and effectiveness. the roots (hydrogen pyroxide H2O2, proteins However, applying this adjuvant to immunize content, catalase and peroxidase activity). The large animals requires using large quantities of results show that lead induced an increase venom antigens to produce sufficiently effective. inhydrogen peroxide production, proteins Improving Alum efficiency in stimulating the content and an enhancement in the activity of production of neutralizing antibodies seems antioxidant enzymes (catalase and peroxidase). It possible through the formulation of a has been demonstrated that H2O2 production nanoparticulate adjuvant based on the chemistry play an important role in case of stress, the of Alum. In this study, synthetized aluminum

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hydroxide nanoparticles (Nps) are characterized P122 and tested for their capacity to induce an effective specific immune response and lower Isolation and characterization of endophytic inflammation, compared to classic Alum to actinobacteria associated with Artemisia sp improve the current immunotherapy approach. from Batna region Alum-Nps were synthetized and characterized by Scanning Electron Microscopy, Transmission Belkis Menaa1*, Sarah Rahal1, Djamel Chekireb1. Electron Microscopy, and Fourier Transform 1 Infra-Red spectroscopy to compare the shape, Laboratory of Applied Biochemistry and Microbiology (LBMA) - Badji Mokhtar Universityof Annaba, Faculty of size and chemical composition with the Alum. Sciences, Department of Biochemistry BP12 Sidi Amar Annaba Two immunizing formulations were prepared 23000- Algeria. using cerastescerastes venom conjugated to *[email protected] either Alum or Alum-Nps, then injected to two groups of rabbits according to an established schedule. Blood samples were used to study Abstract inflammatory cells activation statue via cell count Cancer disease is one of the most serious human and specific enzymatic markers activity. Specific health problems in the world and actually many immune response was also assessed by research studies are focused on the discovery of peripheral lymphocytes count and specific natural antioxidant and anticancer agents from antibody titers evaluation. After one month from microbial sources. The phyllum Actinobacteria is the last immunization, was collected from rabbits a great source of antitumor compounds andthe were challenged with different lethal doses of genus Streptomyces is a good source of venom to evaluate their effectiveness in anticancer drugs andexhibit strong antioxidant protecting in non-immunized mice. Results activity cytotoxicity against colon cancer cell. showed that Alum-Nps have 50-200 nm spherical Actinobacterial diversity has been extensively shapes with the same chemical composition of researched in a variety of environments because Alum, induced lower inflammation, greater of its unique biotechnological potential. effectiveness in activating lymphocytes and However, such studies have focused mainly on higher anti-venom antibody titers compared to soil communities and recently, endophytic plant Alum. This observation may be due to the tissue actinobacteria, have attracted the profound infiltration of Alum-Nps to the attention of researchers in recent years. Our aim secondary lymphatic organs. These antibody is the study of biodiversity and the development titers are the origin of the more potent passive and exploitation of the endophytic actinobacteria immunoprotection of sera extracted from Nps- of Artemisia medicinal plant harvested from Arris immunized rabbits until 1LD50 of native venom. Mountain in the Batna region. Our interest is to Altogether, these results showed that reducing look into the potential antioxidant and anticancer the size of classic Alum to nanoparticles activities of Streptomyces from this increased its activity and security profile for a underexplored areas. To search for new taxa; we more ethical and effective anti-venom sera collected for this study 12 plants of Artemisia spp, production. of Djebel Arris (Batna-Algeria).Isolation, purification and morphological study of the Keywords: Snake envenomation; Aluminum isolates were performed using three specific hydroxide nanoparticles, Alum adjuvant, culture media: ISP1, ISP2 and ISP3 (International immunotherapy. Streptomyces Project). The cultures are observed after 15 days of incubation at a temperature of 28 ° C. Microscopic observations of the isolates allowed us to select colonies with an appearance similar to actinobacteria (presence of filaments and spores).Forty-six (46) actinomycetes were selected and cultured on ISP1 and ISP3 specific media and categorization was performed according to colony color, pigment production and diffusion, and presence of inclusions. The results of the culturing of isolates on ISP1 and

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ISP3 allowed us to classify them in 8 different of between 10 and 14 mm, and a strong groups according to the color of aerial antifungal activity against yeast Candida albicans mycelium .We note that the gray series ATCC 10231 with inhibition zones ranging from represents the major group, which is followed by 19 to 24 mm. MICs were 100 μg / mL for all the white series generally it is the group of bacteria and 10 mg / mL for yeasts. The Streptomyces that have the most these two evaluation of the antiradical activity showed that colors. The abundance of gray series in the the phenolic extract has an IC50 of DPPH actinobacteria collection has been reported in interesting of the order of 0.182 mg / mL. The several studies. These tests allowed us to group results confirm that Coffea arabica coffee beans the majority of isolates among the genus have potent antimicrobial and antioxidant Streptomyces. We can deduce from these activity due to their high secondary metabolites. preliminary analyzes that the genus Streptomyces is very present in association with Keywords: Coffea arabica, phenolic extract, the medicinal plants of the Arris region antibacterial activity, antifungal activity and ecosystem. antioxidant activity.

Keywords: Arris mountain; endophytic actinobacteria ; Streptomyces. P124

P123 Antistaphylococcic compound produced by thermophilic Bacillus licheniformis LMB3704 Phenolic extract, study of antimicrobial and isolated from the hot spring Dbagh in Algeria antioxidant activities of Coffea arabica green seeds Nadia Aissaoui 1*, Nihel Khlouche Khelil1,2, Mohammed Aissaoui3, Fatima Nas1.

Mohammed Aissaoui 1*, Said Barek 1, Mohammed 1LAMAABE Laboratory Tlemcen University, Algeria . 1 Nadjib Rahmoun . 2 Laboratory of Experimental Surgery, Dental Surgery Department, Faculty of Medicine, Tlemcen University, Algeria . 1 Laboratoire Antibiotiques Antifongiques : physicochimie, 3 Laboratoire Antibiotiques Antifongiques : physicochimie, synthèse et activité biologique .Université de Tlemcen. EX. synthèse et activité biologique Tlemcen University, Algeria. Complexe Biomédicale, Imama. Bloc B, Tlemcen 13000 – [email protected] Algérie. [email protected] Abstract Abstract Natural plant substances have multiple interests The rapid emergence of multi drug -resistant in biotechnology and in different industries. microorganisms poses a major threat for human Coffee is considered the second foodstuff in the health. The aim of this study was to determine world. Several studies have shown that coffee has the potency of crude extract produced by beneficial biological properties for humans. In thermophilic bacterium LMB3704 isolated from this work, In this work, we investigated the the thermophilic environment, Dbagh hot spring antimicrobial and antioxidant activity of the in Algeria against two MDR human pathogens. phenolic extract of Coffea arabica. The The first objective was to combine cultural antimicrobial activity tests were performed using approaches (Gram reaction, API 20E, enzymes thse agar disk diffusion method and the MIC production and physiological characterization) determination against 14 bacterial strains with molecular methods (sequencing of 16S (Gram-positive and Gram-negative) and three rRNA) in order to specify the taxonomic rank and reference Candida albicans yeasts. The evaluation discriminate isolate up to subspecies. The second of the antioxidant activity of this extract was objective was to highlight the antimicrobial carried out by the DPPH free radical scavenging potential and determine the chemical method. The results of the antimicrobial tests composition of extract. Supernatants from isolate showed that the phenolic extract has the best LMB3704 were extracted with ethyl acetate. The antibacterial activity against Gram-positive antimicrobial activity was tested by the Radial bacteria with diameters of the zones of inhibition Diffusion Assay (RDA) technique against two

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MDR bacteria: Pseudomonas aeruginosa P125 (NR_0754828.1) and Staphylococcus aureus (NR_075000.1). Gas Chromatography–Mass Contribution to the assessment of metallic Spectrometry (GC–MS) was used to determine pollution of the quality of marine waters by the composition of extract. Partial phylogenetic the use of two bioindicators sole Solea solea analysis of the amplified 16S rDNA gene (number (Linnaeus, 1758) and pageot acne of base pairs) showed that the isolate named Pagellusacarné (Risso, 1827) sins in the LMB3704 was attributed to Bacillus licheniformis littoral oranais with a similarity 99.64% according to the EZBiocloud database. The sequence was deposed Ali Tabeche1*, Fatma Belhoucine1, Amel Berrebbah- in GenBank data base under number MK027022. Alioua1, Sultana Bouhadiba2, Lahouaria Belhabib1, On the basis of cultural approaches, significant Khaled Touati1, Sakina Gherbi1, Zohra Zaiter1 differences were observed compared with Bacillus licheniformis ATCC 14580. The isolate 1 University of Sciences and Technology Oran-Mohamed Boudiaf USTO-MB,ElMnaouar, BP 1505, Bir El Djir 31000, exhibited extracellular amylase, protease, lipase, Oran, Algeria.. nitrate-reductase and cellulase activities at 55°C. 2 Environmental Monitoring Network, Department of Biology, The ethyl acetate extract of Bacillus licheniformis Faculty of Natural Sciences and Life, University of Oran 1- LMB3704 showed high biological activity just Ahmed Ben Bella, Algeria against Staphylococcus aureus (NR_075000.1)., [email protected] multidrug-resistant using Radial Diffusion Assay (RDA). Characterization of the antibacterial Abstract compounds produced by LMB3704 strain using Gas Chromatography–Mass Spectrometry (GC– The introduction by humans, directly or MS) analyses showed the presence of 1- indirectly, of substances or energy in the marine Fluorododecane at 48.166%. According to our environment causing adverse effects such as biological studies, we showed in this work that nuisance against biological resources, risks to this compound was produced at 55°C and human health, obstacles to maritime activities, an possess specific antibacterial activity against alteration of the quality of seawater. The present Staphylococcus aureus (NR_075000.1). Dbagh hot study consists in evaluating the quality of the springs are poorly understood and are a source Oran littoral using a biological approach based of biotechnological potential of Bacillus genus. on the estimation of the contents and the These results also indicate the importance of bioavailability of two metallic trace elements therapeutic and industrial value on an copper and lead in two species of teleost fishes: unprecedented scale from hot spring. To the best the Soleasolea (Linnaeus, 1758) and of our knowledge, this is the first report showing Pagellusacarne (Risso, 1827) sins in the bay of production of bioactive molecules as Oran. Sampling was carried out over a six-month extremolytes compounds biosynthesized at 55°C period from October 2018 to March 2019 on 90 by thermophilic Bacillus. individuals. The analysis of metals focused on the entire population, two organs were removed, Keywords: Thermophilic Bacillus licheniformis; muscle and liver. The determination of the hot spring; Antistaphylococcic compound; GC– metals was carried out by flame atomic MS. absorption spectrophotometry (S.A.A). The comparative analysis of the average lead and cupric concentrations in muscle and liver tissue shows that the importance of metal accumulation is in descending order of the following: liver> muscle in the two species caught in the Bay of

Oran. Several studies have shown that trace metal metals accumulate mainly in organs with a large metabolism as the case of the liver. Our results indicate that there is a trend of increasing average levels of both pollutants in spring compared to winter. Our results also coincide with those found in several species caught in the

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western Algerian coast. From the comparative Hhcy is observed in fasting state either because study of our two species, it is generally apparent of the mutation of the gene encoding MTHFR, or that sole tends to average high accumulation of because of an alteration of vitamin B9 or B12 heavy metals compared to the page, this can be status (Guilland et al., 2002). The aim of this explained by its position in the food chain and its study was to evaluate a possible association mode of benthic life. The major reservoir of between hyperhomocysteinemia and the pollutants in the marine environment is different genotypes of the polymorphism C677T sediment. Our study showed that the Sole has an of MTHFR gene, based on clinical and average accumulation of heavy metals (Cu, Pb) biochemical data, in pathological population, generally higher than that of the pageot mite, residing in Aures region (Northeast Algeria). The which can be explained by the position of present study included 14 young patients (under Soleasolea in the food chain and its way of life 40 years), all patients were in the chronic phase benthic. In perspective, it is interesting to of myocardial infarction (MI). Different biological develop in the future more in-depth research to methods were used to evaluate the biochemical better assess the current level of pollution and its and genetic status of our study population. The impact on the coastal environment, by Competitive immunoassay was used for the identifying mechanisms of detoxification by determination of vitamins (B9 et B12). The certain proteins. identification of C677T polymorphism was determined by using the Real-Time Polymerase Keywords: Solea solea, Pagellusacarne, metallic Chain Reaction - Fluorescence Resonance Energy pollution, Oran bay. Transfer (Real-Time PCR -FRET) technic; it was carried out with a Light Cycler ® 480 II Instrument. Our results show no significant association of moderate Hhcy with different P126 genotypes of C677T polymorphism (P=0,117). However, a decrease in folate and B was Association between the C677T 12 recorded with moderate Hhcy for (TT) genotype polymorphism of MTHFR, blood folate and but remains non significant (P=0,901, P=0,304 vitamin B12 deficiency, and elevated serum respectively). This could indicate the deleterious total homocysteine in young patients with effect of the muted genotype on the myocardial infarction in Aures region concentrations of tHcy, B12, and B9 in patients. (Algeria) Our results are in agreement with those of Tanis and his collaborators (2004), who have shown Hayat DJAARA1*, Yamina OUARHLENT 2 that genotype (TT) is associated with an increased risk of MI, only when the folic acid 1Biotechnology’s Laboratory of the Bioactive Molecules and the Cellular Physiopathology Department of Biology of status is low. A study recently conducted by Liu Organisms, University of BATNA-2-, ALGERIA. et al., (2015) including patients with coronary 2 Department of Medicine, University of BATNA-2-, artery disease, from different categories, ALGERIA.University of Batna 2 - 53, Road of Constantine. revealed that Hhcy was independently associated Fesdis, Batna 05078, Algeria. with the severity of coronary artery disease and [email protected] significantly correlated with low levels of folic acid. In the C677T mutation of the MTHFR gene, Abstract the MTHFR enzyme becomes thermolabile and MTHFR activity decreases by 65% and 30% Homocysteine (Hcy) is one of the biochemical respectively in homozygotes mutant (TT) and markers of cardiovascular disease, many studies heterozygous (CT) (Frosst et al., 1995). In have already demonstrated a link between Hcy conclusion, our study supports the hypothesis and cardiovascular disease (Vesin et al., 2007). that polymorphism C677T MTHFR can be a risk The 5,10-Methylenetetrahydrofolate reductase factor for MI in the presence of moderate Hhcy. (MTHFR) is one of the main regulatory enzymes We therefore suggest a strategy for the biological of homocysteine metabolism (Frosst et al., 1995; exploration of Hhcy to allow the assessment of a Klerk et al., 2002). The hyperhomocysteinemia moderate or intermediate Hhcy as quickly as (Hhcy) is common, and may be caused by genetic possible, in order to inform the general public in disorders, nutritional or therapeutic. Moderate our region (Aures) and country about the risks of

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the disease. Furthermore, genetic investigation of P128 various MI markers would be helpful to treat and predict the risks of this pathology. Natural products to prevent oxidative stress and cancer: dose-manner response effect in Keywords: Algeria; Aures; genetic human leukemia cells (HL60) and cervical polymorphism; Hyperhomocysteinemia; MTHFR cancer cells (HeLa) C 677T; Myocardial infarction; RT-PCR. Abdelouahab Dehimat1,2*, Ines Azizi1, Véronique Barragan-Montero3, Bachra Khettal2.

P127 Département des Sciences de la Nature et de la Vie, Faculté des Sciences Exactes et des Sciences de la Nature et de la Vie, A Computational Tool for Calculation of Université de Biskra, Biskra 07000, Algérie. Laboratoire de Biotechnologies Végétales et Ethnobotanique, Absorbed Dose to Patients Following Bone Faculté des Sciences de la Nature et de la Vie, Université de Scan with 99m Tc‐Marked Diphosphonates Bejaia, Bejaia 06000, Algérie. LBN, Université de Montpellier, Montpellier, France. Moncef ATI1*, HadjOthmaneSaliha2, [email protected] BouzidMokhtaria3.

1Faculty of medecine, University Of MostaganemAbd El Hamid Abstract IbnBadis. 2University Of Sciences And Technology Of Oran-Mohamed Cancer is a leading cause of morbidity and Boudiaf. mortality worldwide. Chemotherapeutic drugs 3 University Of Sciences And Technology Of Oran-Mohamed kill rapidly proliferating cancer cells, but these Boudiaf. drugs also damage normal cells and result in a [email protected] high incidence of complications. Overproduction of free radicals and reactive oxygen species lead Abstract to various cancers and degenerative diseases caused by oxidative stress. Phytochemicals are A computational toolkit called (IntDos) has been considered suitable candidates for anticancer developed to calculate absorbed dose in nuclear drug development due to their pleiotropic medicine technology based on the MIRD actions on target events with multiple manners. Pamphlet, using previous work based of The aim of this study is to display the antioxidant International Commission on Radiological and the anticancer properties by natural Protection (ICRP) established a committee on products from endemic plant of the Algerian permission internal exposure. Where the must Sahara against human cancer cell lines and its radionuclides used in therapy or in imaging can growth inhibition was followed through dose- enter human body, cause damage effect and lead manner. The anticancer of NPs towards cancer to health risk. on the other hand, radionuclides cells was evaluated by MTT assay in dose and can cure cancers by irradiating the tissues. In this dose-time manners against cervical cancer cells work, The toolkit includes a Matlab (The (HeLa) and human leukemia cell (HL60), Mathworks, Natick MA) is used to calculate the comparing to the paclitaxel (Taxol), as positive radiation doses delivered to the organs during standard. Whereas oxidative stress inhibitory the nuclear medicine exam. A biokinetic models assays were measured by spectrophotometry and the dosimetric formula generalized by ICRP methods scavenging radical (DPPH, Superoxide and Committee on Medical Internal Radiation scavenger assays) and acting as reducing and Dose (MIRD) are used. At last, a several chelating agents assays. The NPs exhibited a calculation of nuclear medicine imaging case is marker anticancer effects on cancer cells in a presented and internal absorbed dose obtained dose- (1 to 100 μM) and time-dependent manner were discussed in detail. on decreasing the growth rate of cancer cells, and Keywords: Monte Carlo, Dosimetry, radiation, had little inhibition to normal keratinocyte (No ICRP cytotoxicity inhibitory activity on normal cells by live dead method has been reported after 72 hrs of exposure). In addition, the NPs had a significant effects NPs as antioxidative agents

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were exhibited an important role in the during the years 2017-2019; using 189 prevention of the damaging effects of free structured interviews, field observations, radicals by their neutralization through donation preference and direct matrix ranking with of hydrogen to manger the oxidative stress and traditional medicine practitioners of Aloe vera in diminish its damages in cell health process as western Algeria. The results obtained in this well as cancer. Experimentally, a number of study also showed that 94.4% of aloe gel was the phytochemicals have been shown to attenuate most used part. The recipes are mainly prepared angiogenesis, migration, adhesion, invasion, and from fresh plants and fresh gel. In addition, metastasis and/or to promote apoptosis and among all the diseases treated: skin diseases and autophagy of cancer cell lines. Phytochemicals skin problems are the most common and gastric belong to different chemical categories including and digestive problems 53%.Cancer prevention, polyphenols such as cyanidin‑3‑glucoside, and in the first stage 34%, use after epigallocatechingallate derivatives, quercetin, chemotherapy to protect the skin to side effects myricetin, rutin, the isoflavonebarbigerone. 9%. According to MILAN 2011. Tumors from Based on results derived from this study, we patients who received Aloe vera were more conclude that NPs had a potential anticancer quickly reduce. Aloe vera used for cancer and effects in low time of exposure in HL-60 and tumor prevention or treatment; 69.05% of these HeLa cancer cell lines. The observed pro-cell were cited as being used for the treatment of death activities of NPs coupled with its low tumors and cancer in general and 30.95% for toxicity towards normal cells indicate that this specific tumors or cancers. The vulnerability of natural product could be used as a future Aloe vera plants to over exploitation and anticancer modality. Beside, we can consider NPs extinction needs to be dealt with seriously.Our as a potential agent’s with dual action in the results can be considered as a source of development of new drugs to protecting cells information for scientific research in the field of from oxidative stress and cancer complications. phytochemistry and anti-cancer pharmacology. Therefore, further analysis to determine the Keywords: Aloe vera, Ethnobotany, Traditional exact mechanism of action and in vivo studies on animal models are warranted. medicine, West Algeria.

Keywords: Oxidative stress; Cancer; Cytotoxicity; Natural product. P130 Oxidative stress and nutritional profile in an P129 affected population of colorectal cancer in region of Tlemcen Ethnobotanic Study Of Aloe vera In Western Algeria Nabila Hamidi1*, Naima Badid1.

1 Fedoul F F*, Meddah B University of Abou Bekrbel Kaid of Tlemcen, Tlemcen, Algeria, 13000.

1Laboratory of PPBIONUT Laboratoire de Bioconversion ; Génie microbiologique et [email protected] sécurité sanitaire, Faculté des Sciences ; Université Mustapha Stambouli -Mascara [email protected] Abstract

Colorectal cancer (CRC) is the second largest in Abstract terms of incidence and mortality in Algeria. The For a very long time, traditional medicine has objective of this work is the study of risk factors been risky to be neglected or to disappear little for CRC in a population at western Algeria. A by little, that may be due to the intervention and case-control study was conducted among 70 evolution of modern medicine. In this study, we people, including 30 patients and 40 controls, to describe the medicinal plants Aloe vera that have determine some parameters of oxidant / been reported to be antitumor agents and that antioxidant status in CRC cases. Markers of have been used in ethnobotanic research in oxidative stress (Malonaldehyde (MDA), the Algeria. Ethnobotanical data were collected protein carbonyl (PC) and markers of antioxidant

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enzymes (catalase, glutathione (GSH) were anticancer drug. Among the results of this review investigated in cancer patients and controls. Our it was found a growing number of studies (all results show that Malonaldehyde and types of articles), it clearly indicate that mass carbonylated proteins are higher in cancer spectrometry imaging (MSI) is a valuable method patients than in controls with 42.85% and 80% for direct structural analysis of molecules, it respectively, a deficit of GSH (83.3%) and allows to quickly identify or/ and optimize a drug catalase (79.31%). Scientific studies highlight the candidate , pharmacological target screening and interaction between markers of oxidative status, quantification. It was found that the most lifestyle and genetic predisposition to colorectal publications discussed the use of MALDI-MSI; it cancer. In conclusion, this study suggests that the allows identifying specific drug/target protein CRC is associated with elevated oxidative stress interactions in tissue sections carrying typical lifestyle and genetic predispositions are regions of diseased cells. Other types of identified as risk factors for CRC. instruments, such as secondary ion mass spectrometer (SIMS) and desorption Keywords: Colorectal cancer; Oxidative stress; electrospray ionization (DESI) mass The Malonaldehydes; The Protein carbonyl. spectrometer, can be employed within MS imaging (MSI). These techniques provide also invaluable information relating to treatment P131 efficacy. In fact, latest major developments of new methodologies and applications touching all Mass spectrometry imaging an innovative tool aspects of MSI in this field across multiple in anticancer drug discovery research centers are considered as a source of promising method in the drug discovery as well Zineb Chellouai 1,2*, Hussein Benachour 1, as the drug development process within the Abdelmadjid Bouderbala1, Rachid Moussaoui 1,2, pharmaceutical industry. Available evidence Mourad Nachi 2,3. shows the important opportunities for understanding about the mode of action of drug Laboratoire de Chimie Analytique, Dépatrtement de activity in cancer disease. Recently, the Pharmacie, Faculté de Médecine, Université Oran 1, BP 1510 EI Menaouer 31 000, Oran, Algeria. significant technological improvements of the Service de Biochimie, Etablissement hospitalo-universitaire performance of MSI are contributing in the 1èr Novembre 1954, BP N° 4166 Ibn Rochd Oran, Algeria. adaptation of this technique more and more in Faculté de Médecine, Université Oran 1, BP 1510 EI Menaouer anticancer drug discovery. 31 000, Oran, Algeria. [email protected] ; [email protected] Keywords: Cancer; Discovery; Drug; Mass Spectrometry Imaging. Abstract Mass spectrometry imaging (MSI) is a powerful P132 tool for bioanalysis at many stages of the anticancer drug discovery process including Does Argan Oil affect Nitroso compounds pharmacological target screening and evaluating formation? the distribution of drug and drug metabolites in cells and tissues. This technique has both Fatima Zohra Chenni1 *,Gunter G C Kuhnle2 * qualitative and quantitative uses. A variety of 1Department of Biology, Biotoxicology Laboratory, SNV techniques can be used to detect and visualize Faculty of DjilaliLiabes of Sidi Bel Abbes University, BP the spatial distributions of biomolecules directly 89,22000, Algeria from biological tissues. In this mini review we 2Department of Food & Nutritional Sciences, University of aimed to highlight the MSI principles with Reading, Reading RG6 6AP, United Kingdom *[email protected] examples of their application in anticancer drug discovery. A review was carried out by searching PubMed® /Medline® databases of mass Abstract spectrometry imaging (MSI) in anticancer drug discovery, for the last ten years. Search terms Recent epidemiologic, experimental, and included mass spectrometry imaging (MSI), mechanistic evidence suggest that dietary heme

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iron has a central role in the promotion of cellulases and proteases are the most sought colorectal cancer by promoting the formation of after thermophilic enzymes in various industrial N-nitroso compounds. However, diet rich in sectors. In this perspective, this study aims to fruits and vegetables may reduce the risk of highlight the enzymatic potential of strains colorectal cancer. Argan oil is among many isolated from thermal springs of Saida (Hamam natural products with several health benefits and Sidi Aissa) and Mascara (Hamam Bouhanifia). wide range compounds that are very beneficial. From the samples of thermal waters, samples This oil takes origin from argan tree, endemic to were stroke on different agar plates (M9, Mossel, Morocco and Algeria. The aim of this study is to nutrient agar and TSA) and incubated (24h at assess the effect of argan oil extracts on the 45°C). The isolates obtained were screened for modulation of the in vitro formation of ATNC cellulolytic, proteolytic (gelatinase and (Apparent Total Nitroso Compounds), potent caseinase), amylolytic and lipolytic activities. agents in the etiology of colorectal cancer. In Following this selection, the best isolates (HMZ, vitro incubations in simulated gastric juice were H2, H1) were selected identification conducted to assess the effect of polyphenols (phonotypically), through the macro- from argan oil on ATNC formation. Different morphological and micro-morphological concentrations of argan oil extracts were characteristics (Gram stain, spore observation) incubated with bovine serum albumin, HCL 1M and biochemistry (indole, catalase, cytochrome and 1.56 M of NaNO2 at 37°C. Samples were oxidase, urease and fermentation of sugars - collected and analyzed by chemical denitrosation glucose, lactose, sucrose, mannitol, Simmons with chemilumine science detection for different citrate and the study of mixed acid fermentation types of nitroso compound 975. The preliminary pathways). The three isolates were tested for results of this study show that argan oil has no their ability to grow at different pH and relevant protective effect on the in vitro temperature (60, 70 and 80 ° C). At the end of the modulation of ATNC compounds isolation, 20 strains were obtained (with formation,nitrosothiol and nitrosyl haem different macromorphological characteristics). products. Although this findings suggest no The best isolates showed good potential in terms evidence of argan oil effect, this remains of enzymatic activities. The strains grow well at beneficial oil for human health and the great pH 10 and pH 7, but were unable to grow at pH 4, source of healthy dietary fat. as well as above 70 ° C. By means of a heuristic identification by the ABIS online program, the Keywords: Nitroso Compounds; ATNC; Argan interpretation of the phenotypic profiles allowed Oil; Simulated Gastric Juice; Cancer Colorectal. to assign the strain HMZ to the species Bacillus licheniformis with a similarity score of 81.4%, the strain H2 to the species Pseudomonas P133 alcaligenes with a score of similarity of 84.2% Screening of lipases, amylases, cellulases and and finally the strain H1 to the species Bacillus tequilensis, the latter with a similarity score of proteases from thermophilic Pseudomonas 84.3%. According to their properties (optimal and Bacillus strains pH, optimal temperature, thermostability, specificity), these enzymes could be the object of Belaouni Mohammed Amine1, Chentouf khadidja1 a complete analysis, including the optimization of

University « Mustapha Stambouli » of Mascara. their production and purification. *[email protected] Keywords: Protease, Amylase, Lipase, Cellulase,

Thermophilic bacteria, Bacillus, Pseudomonas Abstract

Thermophilic enzymes have several advantages over mesophilic enzymes, in particular because of their greater resistance to thermal and chemical denaturation. Demand for thermozymes for biotechnology applications is growing rapidly. Lipases, esterases, amylases,

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P134 P135 Effect of solvents on antibacterial activity of Use of FISH sperm technique for the study of Tirmanianivea (Desf.) Trappe from Algeria aneuploidies in men with abnormal sperm parameters Samir Neggaz1*, Mohammed Reda Missoum1, Zohra Fortas1, Nadia Kambouche2 and Mohammed Chenni3 O. Liamani*1, LH. Labga1, S. Moulessehoul1, I. Benzeguir2. 1 Laboratoire de Biologie des Microorganismes et de Biotechnologie (LBMB), Department of Biotechnology, 1 Departerment of Biology, university Djillali Liabes, Sidi Bel University Oran1 Ahmed Ben Bella, Oran, Algeria; Abbes, Algeria. 2 Laboratoire de synthèseorganiqueappliquée (LSOA), 2 Laboratory of analyses of medical biology Department of Chemistry, University Oran1 Ahmed Ben Bella, * [email protected] Oran, Algeria; 3Laboratoire de Chimie Fine (LCF), Department of Chemistry, University Oran1 Ahmed Ben Bella, Oran, Algeria. *[email protected], +213 550 312 007 Abstract: Today, in industrial countries, about 15 % of the couples are confronted to problems of infertility, Abstract in 1/3 of cases, the etiology is masculine. The The aim of this study is to evaluate the influence production of gametes chromosomal destabilized of different extraction solvent on antibacterial existent of meiotic particular segregation in activity of desert truffle Tirmanianivea(Desf.) every anomaly can explain the infertiliy of these Trappe from Algeria and to determine the best men, partly. The exploration of masculine solvent that can be used to obtain higher infertility remains first of all based on the antibacterial performance. To perform the classical analysis of the parameters of semen. extraction of bioactive compound, Soxhlet However, other techniques aiming at assessing apparatus was used with four solvents like the chromosome equipment, come today to dichloromethane, chloroform, ethyl acetate and supplement it. Our job consists in being methanol.The diameter of growth inhibition was studying, by technology FISH (Fluorescent in situ measured and statistically evaluated using one- Hybridization) for chromosomes 13, 18, 21, X way ANOVA to determine the extract with the and Y, analysis of the rate of aneuploïdies of the greatest antibacterial activity. We investigated gametes of 12 infertile men who were subjected the in Vitro antibacterial activity against two to a spermatic exam. The analysis of the rate of Gram positive bacteria (Staphylococcus aureus aneuploïdies in spermatozoons shows a medium ATCC 6538 and Enterococcus faecalisATCC 2035) rate of 11.78 % and which is significantly more and two Gram negative(Escherichia coli well brought up in comparison with the group ATCC25922and Salmonella witness. In conclusion, FISH can be considered to enterica).Thestatistical analysis showed that the be an additional test for the exploration of type of solvent had an effect on diameters masculine infertility besides the spermatic inhibition (F= 5.39; df = 3; p= 0.0024).The results analysis, playing so a role mattering during revealed that the ethyl acetate extract of T. nivea diagnosis and treatment appropriated by and is very significantly higher (p <0.001) than infertility. As the injection intracytoplasmic by dichloromethane and chloroform extracts, spermatozoons (ICSI) for the patients attained by however the methanolic extract has a very weak OAT, is often used, it is important to inform the effect.Likewise, Staphylococcus aureus ATCC6538 patients if they could have a risk augmented by is the most sensitive todifferent aneuploidies to embryos. extracts,meanwhile, Salmonella enterica is the most resistant. Keywords: Oligoasthenoteratozoospermia, aneuploidies, FISH, infertility. Keywords: Desert truffle, Antibacterial activity, Soxhlet extraction, Solvents, One-way ANOVA.

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P136 grown in full nutrient culture media are incubated under specific conditions that limit cell Halophilic microalgae Dunaliella: biological growth including nutrient imbalance, resource of beta carotene for pharmacology illumination ofhigh intensity and high concentration of salt. Our results show that the Khaled Touati1*, Fatma Belhoucine1, AmelAlioua obtained concentrations of carotenoids produced Berrebah1, Ali Tabeche1. by Dunaliella cells in nitrogen deficient culture medium are according with data reported in 1 Department of living and the environment, Faculty SNV, literature for Dunaliella producing carotenoids University of Science and Technology of Oran Mohamed Boudiaf.31000, Oran, Algeria. under same conditions. Light of high intensity *[email protected] and nitrogen starvation significantly increased total carotenoids content in Dunaliella, but such Abstract accumulation of carotenoids can be also induced by S-starvation and, to less extend (50%), by P- Known for more than a century, microalgae have starvation. The studied microalgae present a never been more interesting than in the last good potential for beta-carotenoid production decade and are constantly attracting attention in and can be considered as a potential novel source all fields, particularly the field of green of bioactive molecules, including a wide range of biotechnology, which is supported by the different carotenoids as the same as several immense possibilities offered by these carotenoids from marine microalgae have been autotrophic organisms for different field from associated with beneficial health effects. pharmacology, bio-fuels to aquaculture. The biodiversity of these organisms is such important Keywords: beta carotene; biotechnology; that we can find them in all the component of the microalgae; recovery. biosphere. More important that in our country, with the diversity of climatic area, the potential is immense. In our work, we are particularly interested in halophilic species and local P136 varieties including the species Dunaliella which in addition to being valuable, its extremophile Impact of cultivation conditions and media character in terms of tolerance to high salinity content on Nannochloropsis gradiation and temperatures makes their exploitation composition uncompetitive in water resources with other activities, particularly the agricultural sector. The Asfouri Nadia Yasmine*, Baba Hamed Mohammed potential of these microalgae in beta carotene as Bey, Abi-Ayad Sidi-Mohammed El-Amine, Lamara Sid- one of the most important natural resource in the Ahmed Chawki. world, and the estimation of this potential, for our local varieties, is highlighted for its future exploitation in the sector of biotechnology, Aquaculture and Bioremediation Laboratory (AQUABIOR), phytotherapy and alternative medicine. Algal Algeria, Department of Biotechnology University of Oran1 samples were collected from slatterns in west Ahmed Benbella, Oran, Algeria. Algeria in Relizane province, and grown with *[email protected] salinity corresponding to the collection site with Jonson medium. Chlorophyll was determined by Abstract heating and extracting with acetone, using an absorbance coefficient. Total carotenoids were Microalgae based biofuels are getting attention determined spectrophotometrically. The due to energy crisis and environmental halotolerant Chlorophyta Dunaliella is well protection. There is potential to increase yields known due to its ability to produce large by manipulating environmental factors, which quantities of b carotene under different cause stress for microalgae. Sources of stress conditions of stress. In fact many conditions that include manipulating environmental conditions affect microalgae growth negatively seem to such as salinity, pH, temperature, and nutrients. induce carotenoid biosynthesis. The production In the present study, we observe how various of carotenoids occurs when microalgae cells nitrogen treatments can impact the growth, lipid

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and pigments accumulation on region of Oran. To improve knowledge on the use Nannochloropsisgaditana. Growth experiments of medicinal plants an ethnobotanical survey in were done at f/2 medium, with an alternative the city of Oran was conducted during the period source of nitrogen, we had a total of five nitrogen of 4 months (February to may 2018) in treatments (NH4Cl, NH4OH, NaNO3, CH4N2O collaboration with the inhabitants of the city and a mixture of all these sources). Each using a questionnaire. All investigations based on treatment consisted of this same concentration of the questionnaire allowed as to gather nitrogen. The Most microalgae are able to utilize information about research area (district, various forms of nitrogen, including nitrate, village), details about informants (name, age, sex, nitrite, ammonium and organic nitrogen sources educational level), scientific name of plant, local such as urea, each nitrogen source is first name of plant, part of the plant used, usage reduced to the ammonium form and assimilated purpose of the plant, dosage, how to use it into amino acids through a variety of pathways. (decoction, infusion, etc), usage period of the Typically, ammonium was known to be preferred plant, side effects of the plant. We interviewed by many microalgae. 500 people of different ages, gender and Significant differences in growth and cellular intellectual levels. Who informed us about the components of microalgal cells have been most medicinal plants used in the city of Oran. observed depending on how the alga will act in The survey allowed us to identify 62 species response to variations in culture conditions, belonging to 30 botanical families. The family of depending on the source of nitrogen, biochemical Lamiaceae is the most dominant followed by composition can also be changed. Apiaceae. In addition, the leaves are the most used part (38%). Furthermore, the infusion was Keywords: Lipid accumulation; the major mode of preparation (66%). In terms Nannochloropsisgaditana; Nitrogen source; of diseases treated, digestive disorders rank first Pigment accumulation. (50%), followed by respiratory diseases (28%). The results obtained will be a very valuable source of information for the region studied. P137 They could be a database for further research in the fields of pharmacology and for the purpose of Ethnobotanic and floristic study of medicinal evaluating the therapeutic efficacy of medicinal plants used in the region of Oran, Algeria plants.

Hafsa Benaiche*1,2, Nadia Bouredja 2, Amel Alioua 2 Keywords: Ethnobotanical study; Medicinal plants; Oran: Traditional medicine. * 1Laboratoire de Génétique Moléculaire et Cellulaire (LGCM), Faculté des Sciences de la Nature et de la vie, Université des Sciences et de la Technologie Mohamed Boudiaf-Oran, Algérie 2Département du vivant et de l’environnement, Faculté des P138 Sciences de la Nature et de la vie, Université des Sciences et de la Technologie Mohamed Boudiaf-Oran, Algérie The research of human Papillomavirus at the *[email protected] level of genital cancers by PCR and Amplicor test Abstract Bouchra Dahmani 1*, LamiaBoublenza1, Medicinal plants are since antiquity used to DalaleBehar1, Malika Mouhoun1, Amira Nahet1, Nabila relieve and cure the human diseases. In fact, their Masdoua1, NafissaChabni2. therapeutic properties are due to the presence of hundreds even thousands of natural compounds 1 LAMAABE, Department of Biology, University of Tlemcen. 2Epidemiology Service, UHC-Tlemcen bioactive called secondary metabolite. In Oran *Corresponding author e-mail:[email protected] the second most important city of Algeria the appeal to the herbalist to cure diseases which Abstract resist to the conventional medicine became Cervical cancer cases are generally consequent recurring in Oran, an ethnobotanical study was upon the infection by human papillomavirus made in order to gather general information (HPV), and a variable proportion of some tricky about the therapeutic uses practiced in the cervical tumors, including vulvar and vaginal

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cancers. The contributions of this project are particularity of being present at very low levels twofold, firstable, to realise a retrospective study in the dissolved phase in view of their for epidemiological profile of genital cancers, hydrophobicity and / or their volatility. The histologically proved during a five (05) years analysis of volatile organic substances in water period (2012/2016) in Tlemcen. The second part requires a particular methodology. Our work is sets out the detection of HPV DNA starting by focused on the development of SPME / GC / MS genital cancers biopsies through classical PCR (Solid Phase Micro-Extraction with Gas and Amplicor test. Overall, in the five (05) latest Chromatography and Mass Spectrometry), which years, 476 cases of genital cancers have been consists mainly of determining whether registered reaching with that a peak of 30% in hydrocarbons are present in the seawaterof the 2014, head lighted by 233 cases (48,94%) cervix Oran littoral. It notably allows a fast, efficient and cancer. The detection of HPV comes by the low-cost analysis of dissolved hydrocarbons in genital biopsy samples, where 70,7% of them different aqueous samples. Sampling of seawater have been revealed positive. The HPV frequency from Oran bay is spread over a period of 11 was at 100% (25/25) in the cervix cancer with months from October 2018 to July 2019. The an average age of 45 years (29-80); 40% (2/5) method used is the SPME / GC / MS. the related to vaginal cancers with an average age of screening carried out by Gas Chromatography 55 years (35-75), 17% (1/6) concern vulvar with mass detector GC / MS in seawater of the cancers with an average age of 47 years (30-80). Oran region has revealed the presence of organic The cervix cancer is essentially targeted by the chemicals: n-C10, Decane ; n-C16, Hexadecane ; strategies of prevention, where screening n-C12, Dodecane ; n-C14, tetradecane ;n-c18, program and vaccination against HPV offer an Octadecane ; n-C20, Eicosane ; n-C22, Docosane, optimism through which genital cancers can be n-C24, tetracosaane ; n-C26, Hexacosan and n- avoided. C28, Octacosane. These found molecules are part of the composition of GAZOLE which it Keywords: Amplicor; genital cancer; HPV; PCR. is classified as carcinogens by the World Health Organization, associated with lung and bladder cancer. Following our present study, we observed that the contamination of the water of P139 the Oran littoral is varied according to the meteorological conditions. Generally, low during Contribution to the assessment of the level of the winter period and high during the summer contamination of the water quality of the period. The results obtained show that the Oran coast by hydrocarbons and waters of the Oran littoral are contaminated by methodological developments for the the hydrocarbons. extraction and analysis of hydrocarbons Keywords: GC / MS;hydrocarbons;Oran littoral; Lahouaria Belhabib*1, Fatma Belhoucine1, Amel SPME. Berrebbah-Alioua1, Sultana Bouhadiba2,Ali Tabeche1.

1 University of Science and Technology Oran-Mohamed Boudiaf, El Mnaouar, PO Box 1505, Bir El Djir 31000, Oran, Algeria. 2Higher School of Biological Sciences of Oran (HSBSO), Oran- Algeria. *[email protected]

Abstract Marine pollution is a topical issue. It poses a risk to human health, by consumption of fishing product.Hydrocarbons are considered as priority contaminants of both terrestrial and marine ecosystems because of their mutagenic and carcinogenic activity. These compounds have the

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P140 P141 Study of seminal oxidative status and sperm Role of Artemisia arborescens on Aluminium DNA fragmentation in men with idiopathic induced hepatic and renal dysfunctions in rats infertility Mansouria belmokhtar*. Omar KHAROUBI Nour El Houda Bousnane 1,2*, Labed Housna3, .Abdelkader AOUES Chennaf Ali 1,2, Mouloud Yahia 1,2. Laboratory of Experimental Bio-Toxicology, Bio-Depollution Laboratory of bioactive molecules and cellular pathologies. and Phytoremediation, Department of Biology, University of Department of biology of organisms, Faculty SNV, Batna 2 Oran1, Algeria. university. [email protected] [email protected]

Abstract Abstract Aluminum is an omnipresent metal in the Oxidative stress (OS) in the male reproductive environment and in our everyday use. In addition tract is now a major concern due to the potential it is determined that it causes deleterious effects mischievous effects of high levels of reactive in the body; the use of plant extracts to correct oxygen species (ROS) on sperm quality and and restore these effects is a simple process function including damage to sperm nuclear without toxic effect to the organism.The present DNA. The aim of our work is to establish an study was designed to investigate the adverse association between seminal oxidative status, effects of sub-chronic exposure to aluminium DNA fragmentation, and semen parameters, and chloride on body weight and some biochemical to search for their possible impact on the risk for parameters and lipid peroxydationin female rats idiopathic male infertility. We conducted a study and to performed the effect of the aqueous on 168 infertile men and 131 fertile controls extract of Artemisia arborescensto reduce and from the city of Batna. Standard semen correcte the alterations caused by aluminium. parameters, DNA fragmentation, seminal Female rats weighing between 110 and 140 g of activities of SOD, CAT, and MDA concentration Wistar strain received intraperitoneal (IP) were evaluated for each participant. Our results injections of aluminum chloride at a dose of 20 show that Seminal activities of SOD and CAT mg / kg. bw twice a week for 6 weeks. 48 rats are were significantly decreased (P<0.001) in cases divided into 4 groups: group of control rats (T) compared to controls, they were also attenuated (non-intoxicated and untreated): received in the majority of infertile men subgroups. We distilled water as drinking water, intoxicated noted that the seminal MDA in the cases was group (Al): receiving aluminum chloride (AlCl3) higher than controls (1.795 ± 0.607 nmol/mL vs administered by intraperitoneal route (IP) at a 1.620 ± 0.533 nmol/mL, P= 0.007). Positive dose of 20 mg/kg.bw and with two injections per correlations between enzymatic activities, sperm week, the group treated intoxicated (AL+EA): parameters, and DNA fragmentation were simultaneously received aluminum chloride detected (P≤0.05), MDA was exclusively (AlCl3) intraperitoneally (IP), at dose of 20 mg / correlated with sperm DNA fragmentation. Our kg. bw and the aqueous extract of the plant findings are in agreement with many literature Artemisia arborescens orally at dose of 200 mg / l data, which indicated that CAT and SOD provide and the group of plant-treated rats (EA) received the most effective antioxidant protection against only the aqueous extract of the plant Artemisia the effects of lipid peroxidation. Our results can arborescens at a dose of 200 mg / l orally. The prove the importance of seminal oxidative status exploration of renal and hepatic function and DNA fragmentation evaluation as useful tools revealed an increase in the level of aspartate for the diagnostics and the management of aminotransferase (AST), alanine idiopathic male infertility. aminotransferase (ALT) and lactate dehydrogenase (LDH) in serum as well as Keywords: SOD; CAT; MDA; idiopathic male thiobarbituricacid-reactive substances (TBARS) infertility; DNA fragmentation. in liver, while, creatinine, urea and uric acid in urine were decreased. In contrast, we observed that the administration of the aqueous extract of 127 Oran 05-07 December

Artemisia arborescensto rats intoxicated with together, our findings suggest that medicinal AlCl3 improvedsignificantly all parameters. plants are widely used by breast cancer patients Weconcludedthatthe aqueous extract of the plant to avoid chemotherapy-related side effects. Artemisia arborescenscan play a protective role Keywords: Breast cancer; North West Algeria; against the harmful effects of AlCl3 toxicity in wistar rat. Medicinal plants; quality of life.

Keywords: Aluminum, Artemisia arborescens, hepatic and renal function, lipid peroxidation. P143

Bioactive compounds of cyanobacteria P142 synechocystissp isolated from west of Algeria

Current status of the use of local medicinal Aicha. ZELLAL1 , Yasmin. ASFOURI1 & Sidi- plants by breast cancer patients in Northwest Mohamed.El-Amine. ABI-YAD1. Algeria 1 Laboratory of Aquaculture & Bioremediation (AquaBior). Fatima Zohra Hantour 1*, Bachir Benarba1. Department of Biotechnology (I.G.M.O campus) Faculty of Natural Sciences and Life, University of Oran, BP Laboratory Research on Biological Systems and Geomatics, 1524, EL M'Naouer - 31000 University of Mascara, Algeria. Oran, Algeria. [email protected] [email protected]

Abstract Abstract The use of alternative medicines is common Cyanobacteria (blue-green algae) are rich among cancer patients in developing countries. sources of structurally novel and biologically Recent studies validated the usefulness of active metabolites. Recent studies indicate the medicinal plants as alternative treatments or to presence of some bioactive compounds in the manage chemotherapy-related side effects. blue green algae which are shown to exhibit Recently, we identified some medicinal species anticancer, antimicrobial, antifungal or anti- used by Algerian breast cancer patients. Indeed, inflammatory and other pharmacological different bioactive molecules from plants were activities. In the present study, aqueous extract found to possess important anticancer activities and methanol solvent extract of isolated marine in both in vitro and in vivo. The present study cyanobacteria strain were tested for aims to identify the medicinal species used by antimicrobial activity against bacterial pathogens Algerian cancer patients, and investigates the a fungus, Gram-positive and Gram- benefits as estimated by patients. A survey of negativebacteria. For preparation of aqueous and medicinal plants for the treatment of breast methanolic extracts, the algal cells were cancer was carried out in northwest Algeria (Sidi harvested every 6-8 weeks, and separated from Bel-Abbès). Information on the plants' names, the medium by centrifugation. Pellets were dried parts used and methods of preparation was and extracted with methanol and water. The collected through a questionnaire among 113 methanol was removed from the extracts using a breast cancer patients. Our results showed that rotary evaporator.Antimicrobial activity was 52% of breast cancer patients used medicinal based on the agar diffusion assay. It was plants. Of them, 4% used commercial observed that the methanol was the best solvent preparations whereas 61% prepared the for extractingofthe antibacterial agents from medicinal plants by different methods Synechocystis sp. Methanol extracts of the blue (decoction, maceration, raw…). Moreover, we green alga Synechocystissp exhibited identified all the species currently used by the antibacterial activity against Gram-positive and patients. The highly cited species were Ephedra Gram-negativebacteria, except Salmonella typhi alata, Curcuma longa, and Atriplex halimus. In however aqueous extract it has no activity several cases, patients considered that using against Gram-negative bacteria. No inhibitory medicinal plants improved both effectiveness effects were found against the fungus and side effects of chemotherapy. Taken Candidaalbicans. Our ability to grow both

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cyanobacterial speciesin the laboratory augurs knowledge of the molecular mechanisms well for the isolation of activecompounds in involved these biological activities. substantial amounts. Keywords: Artemisia, Aqueous extracts, Antibacterial activity. Keywords: Cyanobacteria, bioactive compounds, antimicrobial activity, Synechocystis sp.

P145 P144 Research of Salmonella in chicken offal: phenotypic characterization and test of Contribution to the study of antimicrobial sensitivity to antibiotics of isolated strains activity of the aqueous extract of the white Artemisia Amira Kout 1*, Amani Amamra1, Houria Chettibi 1 Et Radia Boufermes 1. Amani Amamra1*,Amira KOUT1,Besma BEDBOUDI 1,Chahinez SAIDANI 1, 1 Badji Mokhtar University, Faculty of Science, Department of Yassine REGGAMI 2 et Hajira BERREDJEM1 Biochemistry, Laboratory of Biochemistry and Applied Microbiology, Annaba, 2300, Algeria. Department of Biochemistry, Laboratory of Applied [email protected] Microbiology and Biochemistry, Badji Mokhtar-Annaba University,Annaba, 2300, Algeria Department of Microbiology and Biochemistry, Mohamed Abstract Boudiaf-M'Sila University,M’Sila,28000, [email protected] In Algeria, since the 1980s, the emergence of poultry industries has increased the Abstract consumption of animal protein at a very affordable cost. Chicken meat is one of the main Aromatic and medicinal plants possess very reservoirs of Salmonella that are responsible for important biological properties which find many a major zoonotic disease with serious economic applications in various fields, namely medicine, and public health consequences. The objective of pharmacy, cosmetology, agriculture and agro- this study is to isolate, identify and characterize food. The objective of our work is to highlight the Salmonella isolates and determine resistance anti-microbial, in vitro, power of the aqueous profiles for 16 antibiotics. Biochemical extract of the plant Artemisia herba-albaAsso of identification was performed by the API 20 E the Boussaâda region. After obtaining the miniaturized gallery and by two complementary aqueous extract by infusion of the plant studied, tests: Oxydase/Catalase test. The antibiotic we carried-outthe diffusion test on Muller Hinton sensitivity test was performed by Mueller Hinton agar of the discs soaked inthe extract of 9 agar diffusion method. Four isolates are of avian different strains: Proteus mirabilis, Pseudomonas origin collected from chicken offal in two aeruginosa, Klebsiella pneumonia, Acinetobacter markets located in the town centre of the city of baumannii, salmonella typhimurium, salmonella Annaba, and four others are of human origin also enteritidis, Salmonella sp, staphylococcus aureus collected in the city of Annaba. Of which four are and escherichia coli. The results obtained show of the genus Salmonella chloreasuis ssp serovar that the P.mirabilis, P.aeruginosa, K.pneumonia Arizonae, two are of the genus salmonella spp, and A.baumanniis strains are sensitive to the one is Salmonella typhimurium and the last is aqueous extract of Artemisia herba-alba Asso Salmonella enteritidis were identified, these while the S.typhumurium strains, S.enteridis, S.sp, results are similar to those reported by Zhao et S.aureus and E.coli show resistance to the same al. The antibiotic sensitivity test revealed that the extract. These preliminary results show good majority of Salmonella bacteria are resistant to anti-microbial activity of A. herba-alba Asso and beta-lactam antibiotics, kanamycin and open-upexperimental perspectives to explore sulfonamide, they also have strong resistance to anti-oxidant, anti-tumour and anti-infectious macrolide and Cyclines, while they are sensitive activity from the aqueous extract and essential to chloramphenicol and Amikacin, these results oil of this plant and advance towards a better are comparable to those reported in other studies in France, and Ethiopia. The presence of a

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pathogen such as Salmonella in chicken livers or the bibliography. Such as those made by meat and the multi-resistance to the antibiotics BaykanErelet al. in 2012 on the EO of Artemisia makes it a risk to the consumer. It shows the lack arborescens collected in Turkey which is of hygiene in the environment of markets. The characterized by its richness of camphor results of this study open very interesting (33.39%) and chamazuléne (21.05%). The perspectives in particular: the determination of results of the antioxidant activity revealed that the genetic factors responsible for their the EO show no inhibitory effect of the DPPH pathogenicity and multi-resistant to antibiotics radical compared to the standards used. by other phenotypic and genotypic methods. And However, they have preserved the color of β- the research and circulation of new molecules carotene in a similar way to ascorbic acid. Test of (synthetic or natural) that should help against the biological activity of our oils revealed the antibiotic crisis. considerable antioxidant power using the β- carotene bleaching method. Keywords: Salmonella ssp; Chicken offal ; Keywords: Antioxidant activity, Artemisia Biochemical identification ; Antibiotics multi- arborescens, Chemical composition, Essential Oil. resistant.

P147 P146 Construction of a microbrial fuel cell using a Chemical characterization and antioxidant strain of Exiguobacterium sp. isolated from activity of the essential oil of Artemisia Algerian Sahara arborescens from the region of Sidi Bel Abbes Dallel Arabet 1,2*, Sofiane Sedira 1 and Vincent Méjean Said Mohammed El Amin*1, Benyamina 2 Abdelfettah1, Toumi Fawzia1. Laboratoire de Mycologie de Biotechnologie et des activités University of Djilali liabès Sidi Bel-Abbes, Laboratory of eco- microbiennes, Faculté des Sciences de la Nature et de la Vie, development spaces, BP 89, 22000 Sidi-Bel-Abbes, Algeria. Université des Frères Mentouri Constantine 1, Algeria. [email protected] Institut de Microbiologie de la Méditerranée (IMM), Laboratoire de Bioénergétique et Ingénierie des Protéines (BIP), UMR 7281, Aix-Marseille Université, 13402 Marseille, Abstract France. [email protected] The present work focuses on the phytochemical study and antioxidant activity of Artemisia arborescens Essential Oil (EO) which is a species Abstract belonging to the Asteraceae family. The EO was In the midst of the world energy crisis that we extracted by hydrodistillation using a Clevenger are living actually and all the environmental device and analyzed by gas chromatography pollutions risks, finding new strategies to coupled to mass spectrometry. Antioxidant produce clean and renewable energy become a activity was evaluated by DPPH radical real challenge. One of the most important scavenging and β-carotene bleaching methods. alternatives is microbial fuel cells (MFCs). In this The yield of the extraction was 0.40%. By work, we focus to carry out an MFC using an comparing our results with those in the Exiguobacterium sp. strain isolated from Algerian literature, we found that our yields are close to Sahara. The experiments were carried out in those of Said et al. as the oil yields of Artemisia microbial electrochemical device inoculated with arborescens EO from the region of Ain Sefra in Exigubacterium sp. strain in microaerobiosis southwest of Algeria were between 0.40 and conditions. Two kinds of electrochemical systems 0.41% (m/m).The major constituents of the EO were constructed. In the first one, the MFC of the aerial part of are β-thujone (51.31%) consists of a unique compartment system followed by Chamazulene (13.64%) including both of the bioanode and the thencamphor (11.12%) and finally α-Thujone biocathode. The second one is a two (4.41).The chemical composition of ourEOis in compartments system with separated electrodes. accordance with those previously reported by

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The results showed that the unique compartment countries, faces a series of challenges to ensure system is able to produce more important good HCW management. To better manage HCW, electric power than the separate compartment it is important to understand the current system. This could be due to the total separation situation. The present descriptive cross-sectional between the bioanode and the biocathode in the study aims to evaluate, for the first time, the second system. We were able to prove that the process of HCW management in the University power produced by Exiguobacterium sp. is more Hospital of Batna. This is a cross-sectional important than the electric power produced by descriptive study conducted over a period of other bacterium like Shewanella oneidensis which eighteen months, during the year 2015/2016, in is mostly used in this research field. The University Hospital of Batna. The study used Exigobacterium sp. strain isolated from Algerian a variety of data sources; on-site observation and Sahara could present advantages and excellent study of the hospital's archive. The on-site intrinsic properties in energy conversion than observation was carried out with the aim to other models already tested. This work is always appreciate the process of HCW management. It in the early steps. It will be interesting to was carried out using a checklist containing 21 improve the performance of the device and find a judgment criteria, developed according to WHO more sophisticated structure of the MFC. We recommendations and according to Algerian have good reasons to believe that Exigubacterium laws. The study of the archive was carried out sp. isolated from Algerian Sahara could be an with the aim of knowing the quantities of HCW important alternative to produce green energy. generated from the year 2008 until the year 2015. We have sorted and analysed the registry Keywords: Green energy; MFC; Exigubacterium data. The results were then analysed using Excel sp.; Electric power. 2016. The results showed that, the HCW management process in the University Hospital of Batna is inadequate; segregation, collection, storage, transport and disposal of HCW are P148 important steps for good management that are ignored and not properly applied. The University Healthcare waste management status in Hospital of Batna generated an average of Eastern Algeria: a case study in the University 92720.62 kg/year of hazardous HCW during the Hospital of Batna period 2008-2015; of which 56.20% are infectious waste, 36.06% are chemical and/or 1* 1 Housna Labed , Nour EL Houda Bousnane , Amina toxic waste and 7.74% are anatomical waste. The Labed 2, Boualem Mayache 3. average waste generation rate of hazardous HCW 1 Faculty of natural and life sciences, The University of Batna during the period 2008-2015 was 0.40 2, 05000 Batna, Algeria. kg/bed/day. Similar results to those of our study 2 Faculty of natural and life sciences, The University of were recorded in Geneva, Korea, Sudan and Laghouat, 03000 Laghouat, Algeria. Pakistan. A higher waste generation rate than 3 Laboratory of biology, environment and health, Faculty of natural and life sciences, Mohamed Seddik Ben Yahia that of our study was recorded in Turkey, Greece University, 18000 Jijel, Algeria. and in Iran. This study allowed us to evaluate the [email protected] ; [email protected] process of HCW management in the University Hospital of Batna. In spite of the efforts invested by the government for the good HCW Abstract management, the process still inadequate and Healthcare waste (HCW) refers to all wastes insufficient; segregation, collection, storage, generated by the operation of healthcare transport and disposal of HCW are important facilities. The WHO estimates that 85% of these steps for the good management that are ignored wastes are similar to domestic waste and are not and not applied in accordance with the national hazardous. The remaining 15% are considered regulations, or with the WHO recommendations. hazardous and may be infectious, toxic or The implementation of HCW management plans, radioactive. Proper management and safe based on the improvement of the budget system, disposal of these wastes is an important step in the training and capacity building programs of all the prevention of diseases and the protection of HCW handlers and the rigorous application of the the environment. Algeria, like other developing regulations is therefore highly recommended, to

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improve HCW management system and therefore AdenylcyclaseHemolysinAc Hy isapproximately limits the risk to public health and to the 200kDand the filamentoushaemagglutinin (FHA) environment. is 220 kDa. The three strains studied (B.P ATCC 9797, B.P 18323 (SII), B.P ATCC 9340) belong to Keywords: Hospital waste; Medical waste; the type species of B.pertussisare virulentand the Medical waste management; the University SDS-PAGE method allows to identify the four Hospital of Batna. major factors of virulence:Pertussistoxin, Pertactin, Adenylcyclase Hemolysin and Filamentous Haemagglutinin.

P149 Keywords : B. pertussis, virulence factors, pertussis vaccine, immunotherapy Extraction and purification of Bordetella Pertussis Antigens responsible for whoopingcough P150 Khadidja.TaharDjebbar1, 2, 3, Mounia.Allouache¹, Sakina.Linda.Chergui2, Baghdadi.Guidoum2, Contribution to the study of the involvement Sid.Ali.Kacem2, Salim. kezzal1, Fouzia.Benguerguoura1, of rs 2070874 polymorphism of IL4 gene and Rachida. Raache2 and Imene Ouzari3 rs rs3761548 polymorphism of Foxp3 gene in Quality Control Laboratory, Department of Biological Control, the development of keratoconus in a sample Pasteur Institute of Algeria, Algiers, Algeria. of the population of western Algeria Cytokine Laboratory and NO Synthases, Houari Boumediene University of Science and Technology USTHB, Algiers, Algeria. Meteoueki Wafaa 1,2*, Fodil Mostefa2,3, Adda Negaz Faculty of Sciences of Tunis FST, University of Tunis El Nawel2,4, Chiali Abdelillah , Derdour Amine5, Idder MANAR, Tunis, Tunisia. 2,5 1,2 [email protected] Aicha , Zemani-Fodil Faouzia

Laboratoire de Génétique Moléculaire et Cellulaire, Université des Sciences et de la Technologie d’Oran Mohamed BOUDIAF- Abstract USTO-MB, BP 1505, El M'naouer, 31000 Oran Algérie. Agence Thématique de Recherche en Sciences de la Santé One of ourpriorities in the field of vaccinologyis (ATRSS)– Oran, Algérie. the development of a new strategy that Ecole supérieure des Sciences Biologiques d’Oran (ESSBO). harnesses the immune system.In thiscontext and Clinique Chiali, Oran, Algérie Affiliation 4. given the characteristics of the major toxins of Laboratoire de Génétique Médicale Appliquée à Bordetellapertussis, the agent responsible for l'Ophtalmologie, Clinique Hammou Boutlélis Oran, Algérie. [email protected] pertussis,we are interested in the extraction and purification of toxicantigens, in order to produce non-toxicimmunogenicmolecules for Abstract protection,and vector used in immunotherapy due to their mechanisms of action and their Keratoconus is a corneal bilateral dystrophy, biotechnological applications. Our work focuses degenerative, non-inflammatory, characterized on the in vitro study of biochemical, serological by gradual thinning of the cornea that adopts a and molecular characteristics, and an in vivo conical shape and leads to a loss of visual acuity. study of the virulence of three B. pertussisstrains The etiology of keratoconus is still poorly (B.P ATCC 9797, B.P. 18323 (SII), B.P ATCC understood; however, it is probably of genetic 9340)by extracting toxins from bacterial origin. Environmental factors (allergy, eye rub) suspensions followed by concentration by seem necessary for its expression. Keratoconus is membrane filtration [1], [2]. Purification and classified as a non-inflammatory disease; identification of the antigens was performed by however, studies refute this theory after the the technique of polyacrylamide gel discovery of the expression of inflammatory electrophoresis in the presence of sodium mediators such as cytokines and interleukins in dodecyl sulfate (SDS-PAGE). The results obtained the tears of patients suffering from keratoconus. make it possible to identify the major virulence The aim of our work was to demonstrate a factors of B.pertussis:The pertussistoxin (PT) of possible association between allergy and its 46 kD,Pertactin (PRN) is 69 kD, inflammatory mediators and the development of

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keratoconus. For this, we focused on two genetic P151 polymorphisms located at interleukin-4 (IL4) (- 33 C / T) and FoxP3 (-3279 C / A) genes. This Removal of Copper from Aqueous Solutions study was conducted on a sample of 140 with Biomass from Forest by-product individuals, including 70 cases with Keratoconus and 70 healthy controls from western Algeria. Moubarek Mébarki 1*, Kadda Hachem 1,2, Meriem DNA extraction was carried out by the Salting Kaid-harche1

Out technique and the genotyping of the Laboratoire des Productions, Valorisations Végétales et individuals according to the -33 C / T and -3279 Microbiennes, LP2VM, Faculté des Sciences de la Nature et C / A polymorphisms of the IL4 and FoxP3 genes de la vie, Université des Sciences et de la Technologie d’Oran respectively was carried out using the allelic Mohamed Boudiaf, BP 1505, El M’Naouar, 31000 Oran, discrimination technique TaqMan. . The Algérie. Laboratoire de Biotoxicologie, Pharmacognosie et difference in the distribution between the allelic Valorisation Biologique des Plantes (LBPVBP), Faculté des and genotypic frequencies of cases and controls sciences, Université Dr. Tahar Moulay de Saïda, BP 138 was performed by X2 statistical homogeneity cité ENNASR, 20000 Saïda, Algeria. test. Our preliminary results showed at first that [email protected] there is an association between environmental risk factors such as family history with disease, Abstract atopy and eye rub with the development of keratoconus. In a second step, our results Water pollution from heavy metals ions is a main showed that there is no significant association concern in particular in developing countries and between the rs2070874 polymorphism of the IL- Algeria is not an exception. Copper squander 4 gene and rs3761548 of Foxp3 gene and the risk sullying exists in watery waste from copper of keratoconus development in the population of mining, electronic and electrical enterprises, western Algeria. These results would be in favor industries implicated in the manufacture of of the hypothesis considering atopy as a factor computer warm sinks, ceramic glazing and glass indirectly linked to the Keratoconus. Thus, the colouring , severe use of Cu-based agro- association of atopy with keratoconus is chemicals, etc. The World Health Organisation probably due to the inflammation induced by the (WHO) recommends that the quantity of copper rubbing of the eyes. This is the first contribution in drinking water should not go over 2.0 mg/L. to the study of the involvement of atopy in the The utilization of natural material, specifically predisposition to the development of waste matter from farming or industry, has been Keratoconus in western Algeria. It would be to a great extent investigated. The fruit of the oak interesting to study, subsequently, the influence (Quercus sp.), of the family Cupulifers (Fagaceae) of other polymorphisms of the IL-4 and FoxP3 is an acorn with dry pericarp. In this work for the gene and the exploration of other candidate first time the acorn pericarp of two species of genes involved in atopy and inflammation (IL1, Q.ilex , and Q.suber which grows in Norwest IL2, MMP1, MMP9, ...) in the development of Algeria, have been used as a new adsorbent for keratoconus. These studies could also explain the the removal of copper from artificially physiopathological mechanisms at the origin of contaminated aqueous solutions. The this disease. lignocellulosic fraction was characterized before and after adsorption experiments, using Fourier Keywords: Keratoconus; IL4; FOXP3; Allergy; transform infrared spectroscopy (FTIR) to polymorphism; western Algeria. discover functional groups that might be involved in Cu(II) adsorption. The results

showed that the amount of Cu (II) adsorbed on

both adsorbents increased as the initial concentration of Cu (II) increased, from 23.59 to 48.06 mg/g with lignocellulosic fraction of Q.suber, and from 22.56 to 38.19 mg/g with Q.ilex for concentrations from 100 to 500 mg/L respectively. The adsorption data obtained were well described by the Langmuir model

parameters (Qmax= 53.76 mg/g) for

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lignocellulosic fraction of Q.suber, which is higher Paspalum distichum and Poa annua, the species than Q.ilex (Qmax= 36.06 mg/g). Using Panicum repens has taken a remarkable share. Lignocellulosic fraction of acorn pericarp as The dominance of the Poaceae family may be sorbent is a new and very promising tools for explained by the avaibility of this family around wastewater treatment. the dam. Our results corroborate with those found by during their study of the diet of coot at Keywords: Quercus sp; Lignocellulosic fraction; the Djebla dam, they counted 30 plant species Adsorption, Cu (II). consumed, classified into 15 families with the dominance of Poaceae family. in the Djebla dam P152 noted 28 plant species consumed, classified into 17 families, whose Poaceae family was the most Biology and ecology of the Coot Fulica atra in dominant also. This study allowed us to conclude the Djebla dam (Tizi-Ouzou, Algeria) that the adult diet of the coot is essentially vegetarian, its dominated by the Poaceae family, Chahed Soumeya1*, Metna Fatiha2, Brahmi Karima3, which shows the importance of grasses in the Ait Amar Samia4 food of this species.

1,2,3 Laboratory of ecology and biology of the terrestrial ecosystem LE BIOT, Department of animal and plant biology. Keywords: Coot; Diet;Djebla. Faculty of Biological Sciences and Agricultural Sciences. University Mouloud Mammeri, B.P N° 17 RP 15000 Tizi- Ouzou, Algeria. 4Laboratory of Plant production, improvement and P153 protection. Department of animal and plant biology. Faculty of Biological Sciences and Agricultural Sciences. University The refining impact on the nutritional value Mouloud Mammeri, B.P N° 17 RP 15000 Tizi-Ouzou, Algeria. [email protected] and antioxidant potential of some Algerian local cereals

Abstract Nabila Boukhari Benahmed Daidj 2*, Fatima Bensalah 1, Amel Refes1, Fatima Chabane 2, The coot is a diver swimming bird, omnivorous Sabrine Louala 1, Myriem Lamri-Senhadji 1. and opportunistic, very common in lakes, ponds and rivers, its regression disrupts environmental 1 Laboratory of Clinical and Metabolic Nutrition (LNCM). functioning. Its distribution in Algeria includes all University of Oran 1 Ahmed Ben Bella, Algeria. 2 The Higher School of Biological Sciences of Oran (ESSBO), the North including the highlands. For the aim of Algeria preserving this species, we conducted a study of [email protected] its diet, where the knowledge of the latter appears crucial to keep it and identify its role in Abstract the structure and functioning of ecosystems. Our study was carried out at the Djebla dam (Tizi- The purpose of this work was to find out the Ouzou, Algeria) from December 2014 to refining impact on the nutritional value and February 2015 from the analysis of 75 droppings antioxidant potential of some local cereals. Our recovered from the dam, due to 25 droppings per choice was focused on three kinds of the most month. The droppings were harvested with consumed and marketed in Algeria namely forceps and put in paper cornet, while durum wheat (Triticum turgidum), barley mentioning the place and date of collection, fecal (Hordeum vulgare) and oats (Avena sativa). analysis was performed in the laboratory. The Whole grains (WG) samples were obtained by diet of this species was determined using a electric milling of full grains. Refined grains (RG) reference catalog of microscopic structures. We were obtained after a processing. Grain envelope have identified 32 plant species belonging to 16 and Germ were manually removed before different families. The Poaceae family was the milling. The results showed that the WG most dominant with a relative abundance of contained higher nutrient levels than RG. Indeed, 70%. Cyperaceae and Fabaceae accounted for significant differences were noted as follows, 20.88; 2.81% respectively, other families from most important to least important: Fiber occupied a negligible share. Among the Poaceae, (76 - 80%), minerals (32 - 40%), protein (19 - two species were the most consumed, they’re 30%), fats (10 - 34%), water (6 - 18%). No

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difference in total sugars (reducing sugars) level compounds is carried out by two different was observed. However, refining increased methods to confirm the presence of phenolic starch and total carbohydrates levels (on average compounds in the samples. The identification of +7%). The evaluation of the antioxidant capacity flavonoids is carried out according to the revealed a highest total polyphenols technique of Debrayb et al., (1971). The results concentrations in WG compared to RG. The show that the phytochemical tests allowed to estimation of antioxidant potential by DPPH test detect the different families of compounds have demonstrated a strong ability to trap free namely: flavonoids, total and catholic tannins, radicals in WG and RGsimilar to standards (vit C anthocyanins, coumarins, triterpene sterols and and BHT), while FRAP test have demonstrated a also mucilage in 2 spices. The results of the significantly higher trapping capacity in WG chromatography are added to the results of the compared to RG. In conclusion, the refining of phytochemical screening which reveal a cereals leads to an importante nutritional loss considerable presence of phenolic compounds, depending on the concidered nutrient (protein, flavonoids, total tannins and cathechins, fat, fiber, minerals), while the starch level is anthocyanins, coumarins, triterpene sterols and increased. The refining process also leads to a also mucilages in clove extracts and star anise. decrease in antioxidant capacity due to the The evaluation of the antioxidant potential considerable total polyphenols reduction. An reveals that the ethanolic extract of clove increased consumption of whole grains is contains a high content of polyphenols (463 ± 0.7 recommended in order to fully benefits from mg EAG/g) followed by the methanolic extract their nutritional and antioxidant properties and (303 ± 1.02 mg EAG/g). The aqueous extract has to prevent some diet-related deseases (diabetes, the lowest content (181 ± 0.7 mg EAG / g). In star obesity and cancers). anise, the polyphenols in the ethanolic extract represent 73 ± 0.7 mg EAG / g followed by the Keywords: Antioxidant potential; Diet-related methanolic extract (27 ± 1.2 mg EAG / g). The diseases; Refined grains; Whole grains. aqueous extract has the lowest content (4.3 ± 0.2 mg EAG/g). Our results show that clove free radical scavenging (DPPH) is greater with P154 methanolic extract (78.89 ± 1.22%) compared to ethanolic extract (61.46 ± 1, 04%) and the The effects of some bioactive compounds of aqueous extract 21%. In star anise, the ability to star anise (illicumverum) and clove trap free radicals is lower compared to clove (Syrygiumaromaticum) and their antioxidant regardless of the extract used. The results of the potential antioxidant activity of the oils extracted from the two spices, shows that the% inhibition of DPPH Fatima Chabane 1,2, Sabrine Louala1, Nabila Boukhari represents 95.26 ± 0.07% in clove and 92.66 ± Benahmed Daidj1,2, 0.002% in star anise . In addition, the ability to Myriem Lamri-Senhadji1 trap free radicals of clove oil by the FRAP method is higher than that of star anise with values (3.22 Laboratory of Clinical et Metabolic Nutrition, Department of biology. University of Oran 1 Ahmed Ben Bella, Algeria. ± 0.01 and 0.26 ± 0.001), respectively. Clove and The Higher School of Biological Sciences of Oran (ESSBO), star anise have compounds that are very useful Algeria from a nutritional point of view and medicinal by [email protected] their richness in antioxidants and volatile compounds (essential oil). These substances can be used for the production of food additives in Abstract the industrial sector or of healthy foods in the The aim of this study is to estimate the pharmaceutical and nutraceutical sector (food nutritional value of two highly consumed spices supplements). by the Algerian population, cloves Keywords: Medicinal plants; Antioxidants; (Syzygiumaromaticum) and star anise DPPH; FRAP. (Illiciumverum), on the one hand and to identify their main bioactive molecules and evaluate their antioxidant potential by different in vitro tests, on the other hand. The detection of phenolic

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P155 accordance with our results. Superficial mycoses are typically opportunists; the incidence of Prevalence of superficial mycosis in breast mycosis among these patients is expected to cancer women raise, significant challenge remains with regard to the prevention. 1Merad Yassine, 2Messafeur Abdelkrim, 3Moulessehoul Fethi, 1Abdelouahed Khaled, 1 Keyswords: skin mycosis, breast cancer, 1 Bakhouche Samir, Adjmi-Hamoudi Haiet immuosuppresed

*Department of parasitology-mycology, Central laboratory, "Hassani Abdelkader" hospital 2 Department of epidemiology and preventive medicine “Hassani Abdelkader” hospital P156 3 Department of occupational medicine “Hassani Abdelkader” [email protected] Molecular classification of breast cancer in the region of constantine: an epidemiological and immunohistochemical study

Abstract Sara Khelfi1*, Leila Guedjali2*, Souad Haded3*. Mycoses remain a significant cause of morbidity, Affiliations 1.Doctorante a université universitè des freres as the number of immunosuppressed individuals mentouri constantine1,facultè des sciences de la Nature et de increases worldwide, breast cancer patients la vie ,Departement de biologie animal receiving chemotherapy might develop Specialitè :Immunologie moleculaire et superficial mycosis. A cross-sectional study was oncologie,constantine ,Constantine 2500 ,Algerie conducted on 28 breast cancer patients, with Affiliations 2. Masterante a université universitè des freres mentouri constantine1,facultè des sciences de la Nature et de fungal mycosis suspicion, the samples collected la vie ,Departement de biologie animal from each patient were nail scraping and ear Specialitè :Immunologie moleculaire et swabs, all were exanimated under microscope oncologie,constantine ,Constantine 2500 ,Algerie and inoculated into Sabouraud’s tube media. Affiliation 3.Maitre assistante B-UFM Constantine 1 Fungal identification was based on physical [email protected] features of the colonies and biochemical tests (auxacolor). Out of the 28 breast cancers Abstract patients, 8 women had skin mycosis and skin appendage mycosis, so prevalence rate of skin Breast cancer (CS) is the most common female mycosis was 28,6%. 87,5% of positives patient cancer worldwide, ranking first in Algeria for its are employed and 12,5% are housewives. A frequency and mortality. Molecular classification correlation was found between skin mycosis and has distinguished at least four molecular types: the patients aged above 50 years old (p=0,03). luminal A, luminal B, HER2 and basal-like. Our 87,5% of positive cases had otomycosis and 25% objective is to study the phenotypic profile of onychomycosis. Fungal isolates were Candida breast cancer in women with cancer as well as albicans, Candida parapsilosis, Candida the different clinical, immunohistochemical and zeylanoides, Trichosporon sp, Aspergillus niger, therapeutic aspects of different molecular and Aspergillus flavus. Fungal infections in cancer groups. We undertook a retrospective study patients can be further divided into five groups: between October 2016 and December 2017. This superficial dermatophyte infections with little study involved 121 files. The distribution of the potential for dissemination; superficial population according to age showed that the candidiasis; opportunistic fungal skin infections most affected age group is [53-63] years old with with distinct potential for dissemination. 35%. Molecular classification results showed that Necrotizing cutaneous breast fungal infection the most common type was luminal A at 37.19%, were reported, Truppman et al reported a case followed by luminal type B at 27.27%, basal-like series of fungi-associated breast infections after at 19.83%, and HER2 at 15.70%. Breast cancer of 700 augmentation mammoplasties over a nine- luminal type, expressing[ ER], accounts for 70 to year period. The most common causes of fungal 80% of all mammary carcinomas and the work of affections in cancer patients are Candida, and demonstrate that the luminal group A is the Aspergillus, and Fusarium sp, which is in most common with proportions of 58 , 5% and

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54.3% respectively while the distinction is genotyped in a representative sample of 787 observed in the other groups. Molecular subjects from the ISOR (InSulinresistance In classification plays a very important role in the Oran) study (378 men and 409 women aged treatment. This result shows that luminal type A between 30 and 64 and recruited from within the is the most common, and that postmenopausal city of Oran, Algeria). Genetic variants were women are most likely to have breast cancer. considered both individually and when combined This classification is very important in the as genetic predisposition scores (GPSs) for orientation of the treatment. The resulting systolic BP (SBP), diastolic BP (DBP) and molecular classification is expected to better hypertension (HTN) risk. The SNPs in CYP1A1- classify tumors to a personalized therapy. ULK3, HFE, and SH2B3 were significantly associated with BP and/or HTN. The SBP-GPS, Keywords: breast cancer, molecular DBP-GPS and HTN-GPS were associated with classification, immunohistochemistry, hormone higher levels of DBP (+0.24 mmHg p=0.05, +0.23 receptors. mmHg p=0.05 and +0.26 mmHg p=0.03, respectively). Moreover, the three GPSs tended to be associated with a 6% higher risk of HTN. Our study is the first to show that some of the BP loci P157 validated in subjects of European descent were associated (either individually or when Effects of established blood pressure loci on combined as GPSs) with BP traits and/or the blood pressure values and hypertension risk HTN risk in an Algerian population, but to a in an Algerian population sample lesser extent than in European populations. Although larger studies and meta-analyses of 1 Aicha Sarah Lardjam-Hetraf *, Sounnia Mediene- North African populations are needed to confirm Benchekor1,2, Hadjira Ouhaibi-Djellouli1,2, Djabaria the present results, our data contribute to a Naima. Meroufel1, Houssam Boulenouar1, Xavier Hermant3, Imane Hamani-Medjaoui4, Nadhira Saidi- better understanding of genetic susceptibility to Mehtar1, Abdallah Boudjema1, Philippe Amouyel3, HTN. Leila Houti1,5,6, Louisa Goumidi3, Aline Meirhaeghe3 Keywords: BP, GWAS, HTN, ISOR study. 1Laboratoire de Génétique Moléculaire et Cellulaire, Université des Sciences et de la Technologie d'Oran Mohamed Boudiaf, Oran, Algérie. 2Département de Biotechnologie, Faculté des sciences de la P158 Nature et de la Vie, Université d’Oran, Oran, Algérie. 3INSERM, U744; Institut Pasteur de Lille; Université Lille Nord The importance of data collection and de France, Lille, France. 4Caisse Nationale des Assurances Sociales des travailleurs statistical analysis of fishing and flotilla in salariés, Clinique Spécialisée en Orthopédie et Rééducation western coast of Algeria des 5Victimes des Accidents de Travail, Oran, Algérie. 6 Faculté de Médecine, Université Djillali Liabes de Sidi Bel Gherram-Neggaz Malika1, Rabahi Mohamed1, Abi Abbès, Sidi Bel Abbès, Algérie. Ayad Sidi-Mohammed El-Amine 1. 7Laboratoire des Systèmes d'Information en Santé, Université

Oran 1 Ahmed Ben Bella, Oran, Algérie. 1Aquaculture and Bioremediation Laboratory (AQUABIOR), [email protected] Algeria, Department of Biotechnology University of Oran1 Ahmed Benbella, Oran, Algeria. [email protected] Abstract Genome-wide association studies and Abstract subsequent replication studies have pinpointed 29 genetic variants associated with blood This work aims to the study of fishing and its pressure (BP). None of these studies included statistics at the level of the west and the center of North African populations. We therefore looked Algeria, six maritime wilayas (Tlemcen, Ain at whether or not these genetic variants Temouchent, Oran, Mostaganem, Chlef and modulated BP and hypertension (HTN) risk in an Algiers), respectively concerning the ports Algerian population sample. Twenty-nine single (Marsa Ben M'hedi, Ghazaouet, Honaine, Beni- nucleotide polymorphisms (SNPs) were Saf, Bouzedjar, Oran, Arzew, Sidi Lakhdar,

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Mostaganem)over a period stretching from 2010 isolated from them by spreading them on petri to 2015. According to our results, the fishing dishes. Various tests for screening biosurfactant activity of the three segments of the fleet producing strains have been performed. Several increased during the year 2015, as well as the strains were isolated and the strains with the number of vessels of the trawl and sardine fleet highest surface tension reduction powers and (1799) in 2015. The landings of the five major yields were selected for a thorough identification fish groups show a significant dominance of of their growing conditions and the nature of the small pelagic landings (85%) compared with biosurfactants produced, through a series of other major groups in the tenth harbors. The biochemical tests. Strains with halo, indicating oil landings of the port of Ghazaouet as well as the degradation, were isolated, a total of 16 bacterial ports of Beni saf and Bouzadjar occupy the first strains were isolated, each strain was inoculated place compared to the other ports. into an MSM supplemented oil broth and incubated at 37c for 7 days, for biosurfactant Keywords: Data; Harbors; Landings; Statistics. production and each isolated strain was analyzed to reveal bio-surfactant production. Strains with the ability to displace oil from the Oil Displacement test, indicating biosurfactant P159 production, have been selected and stored at - Isolation of biosurfactant-producing bacteria 80c, for previous studies and for further from a hydrocarbon-contaminated area identification of their growing conditions and the nature of the bio-surfactants produced, by a Nassim Lahdiri1*, Farid Dahmoune 2,Abderrezak series of biochemical tests. The biosurfactants Ghidouche3, Djida Ait-Ali 3,Hamza Moussa1 ,Meriem produced in this way could, thanks to their Adouane1, Khadidja Adel1, Amina Abbou 1 , Farida functional diversity, be applied in various Berkani 1,Khoudir Madani2 industrial fields.

1 Laboratoire de gestion et valorisation des ressources Keywords: Biosurfactant; hydrocarbon; bacteria; naturelles et assurance qualité, Université de Bouira, Drissi isolation. Yahia Bouira – 10000, Algérie 2 Laboratoire de Génie biologique des cancers – Université de Béjaia, campus Aboudaou Route de Tichy RN9 – Béjaia - P160 06000- Algérie 3 Laboratoire Biomathématique Biophysique Biochimie et de The teratogenic effects of thalidomide Scientométrie, Campus TargaOuzemmour, Route de TargaOuzemmourBéjaia– 06000, Algérie enantiomers [email protected] Keltoum Dermeche1,2, Noureddine Tchouar1 , Mahdjour Soumicha3 Abstract 1University of Science and Technology of Oran Mohamed Biosurfactants are amphiphilic biomolecules Boudiaf (USTO-MB), Laboratory of Modeling and produced mainly by microorganisms. Their main Optimization of Industrial Systems (LAMOSI), PB 1505 el Menaouer Oran, Algeria. characteristic is to lower the surface and 2University center Ahmed Zabana, Department of Biology, interfacial tension of the solutions. Other than Institute of sciences of nature and life, Rélizane, Algeria. their surface activity, they are slightly toxic and 3University of Sciences and Technology of Oran Mohamed biodegradable and have several other physico- Boudiaf, (USTO-MB), chemical properties, which gives them a wide Laboratory Productions, Plant and Microbial Valuations (LP2VM),PB 1505 el Menaouer Oran, Algeria. field of application. Various studies have [email protected] reported the anticancer activity of biosurfactants, which are involvement in intracellular molecular recognition steps such as signal transduction, cell immune response, cell cycle progression and Abstract: inhibition of certain cell signalling pathways. In Thalidomide is a racemic drug that was this study, samples of petroleum-contaminated administrated to pregnant women between 1956 water were collected from hydrocarbon- –1962. It was immediately withdrawn from the contaminated sites and then pure colonies were market in late 1961, because of its very

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important teratogenesis. Causing serious birth synthesized, purified and characterized by NMR, defects in more than 10,000 children were born Mass spectrometry and X-ray. OurNHC gold with severe malformations, including phocomelia complexes have been synthesized with excellent because their mothers had taken TD during yiels. a study of antileishmanial activity has been pregnancy. While the enantiomer (R) realized giving very good results with our thalidomide is effective against morning sickness, compounds. Six Gold (l)-NHC-complexes show which was the intended scope of the drug, the (S) potent activity against L. infantum amastigote, thalidomide is teratogenic. In this work we study and three of them give encouraging results. difference in structural, electronic and energy of the two enantiomers S and R of thalidomide with Keywords: GOLD; NHC COMPLEXES; 2 methods quantum vacuum by density Imidazolium Salt; antileishmanial activity. functional theory (DFT)and Hartree–Fock(HF) quantum chemistry methods play an important role in obtaining molecular geometries and P162 predicting various properties. Here we only investigate the molecular difference between the Parkinson's Disease: Epidemiological enantiomers and we discuss our results Characteristics of 63 Cases depending on the computational method used. Meroua Horchi1, Dalila Satta 1,Abdelmadjid Hamri 2 Keywords : Thalidomide, Enantiomer , ,Karima Sifi3,Noureddine Abadi3 Phocomelia, DFT, HF . Laboratory of Molecular and Cellular Biology, Department of Animal Biology, Faculty of Nature and Life Sciences, University of Mentouri Constantine, 25000, Algeria. Department of Neurologie, CHU Ben Badis Constantine, P161 25000, Algeria. Laboratory of Biology and Molecular Genetics, Faculty of Nhc gold complexes for biomedicals Medicine, University of Constantine 3, 25000, Algeria. applications [email protected]

Soumia Ftouh1,2 *,Mohamed Belkadi1,Lemya Boughrara3,Sandra Bourgeade Delmas4, Alexis Abstract Valentin4, Catherine Hemmert2, Heinz Gornitzka2. Parkinson's disease (PD) is the second most Université Des Sciences et de La Technologie d’Oran MB, El common neurodegenerative disorder after Mnaouar, BP 1505, Bir El Djir 31000 Oran, Algérie 1. Alzheimer's disease, with an incidence of 6.3 Laboratoire de Chimie de Coordination du CNRS, 205 route de million worldwide, occurring in approximately 1- Narbonne, 31077 Toulouse cedex 04, France2. 2% of people over 65 years of age and older. Université Oran1, El Mnaouar, BP 1524, 31000, Oran, Algérie3. 3.4% aged over 75 years, forms affecting the UMR 152 PHARMA-DEV, Université de Toulouse, IRD, UPS35, young adult also exist. The MP is clinically Chemin des Maraîchers, 31400 Toulouse, France4. manifested by a triad of akinesia, rigidity and [email protected] resting tremor, to which are added many non- Abstract motor signs. The factors at the origin of the onset of PM remain poorly known in the majority Leishmaniasis is a parasitic disease, caused by cases. MP is mostly sporadic, several Leishmania spp., spread by the bite of chromosomal locus then susceptibility genes Phlebotomus sandflies. Leishmania currently have been discovered, and in 10% of cases, the affect 12 million people and around 2 million presence of a family history is found. Few studies new cases occur each year. Resistance to current have been established on PM in Algeria, to this antileishmanial drugs is spreading worldwide end, this modest work aims to establish an and requires an active search for new active epidemiological study on Parkinson's origin of molecules. Gold organometallic complexes have eastern Algeria. A retrospective descriptive study been studies for several biomedical applications was conducted between October 2018 and including their anti-leishmanial activity. A series January 2019 on 63 medical records of of mononuclear neutral gold complexes Parkinson's patients followed in specialized containing N-heterocyclic carbene (NHC) ligands consultation at the Ibn Badis Constantine for anti-leishmanial activity have been University Hospital. Data were collected and 139 Oran 05-07 December

processed with Epi Info 7. The observed results study between these two synthesis methods. are: A slight male predominance. The elderly Then we biologically evaluated the compounds between (50-60 years) are the frequently obtained with reference strains. We have affected by PD. The family form represents 11% therefore established an extremely rapid of cases. Geographic diversity of patients whose; synthesis of chromenes and their analogs 80.95% are from Constantine. These preliminary according to a Michael addition reaction from results show that the PD is answered in Algeria. commercial products and olefins variously A deeper study on a larger sample, with a genetic substituted by the condensation of 2-arylidene study is underway. malononitrile previously synthesized according to a Knoevenagel condensation reaction with Keywords: Parkinson disease; frequency; cyclic compounds having active methylene and epidemiology. having a keto-enol equilibrium. After characterization of the products by the usual P163 spectroscopic methods (Fourier transform infrared spectrometer FTIR and Nuclear Study of the biological activity of nitrogenous Magnetic Resonance NMR 1H and 13C) we heterocyclic compounds evaluated the antibacterial activity of our products on reference sensitive bacteria: Lemya Boughrara1*, Soumia Ftouh2,3. Escherichia coli (gram-), pseudomonas aerogenosa (gram-) and Staphylococcus aureus Université Oran1, Faculté des Sciences Exactes et Appliquées, (gram +) as well as the antifungal activity on Département de Chimie, El Menaouer, BP 1524, Es Senia 31000 Oran, Algérie. yeast Candidas albicans, and on Microsporum Université Des Sciences et de La Technologie d’Oran MB, canis, both by the simple but effective method Faculté de Chimie, department de Chimie Organique "antibiogram". The synthesized products were Industrielle, El Mnaouar, BP 1505, Bir El Djir 31000 Oran, obtained with better yields under microwave Algérie. Laboratoire de Chimie de Coordination du CNRS, 205 route de irradiation compared to conventional heating Narbonne, 31077 Toulouse cedex 04, France. and in very short times (3 minutes instead of 45). [email protected] The study of the antibacterial and antifungal activity of our products has proved very

interesting. Indeed, some of our products Abstract exhibited complete inhibition of bacterial and The synthesis of heterocyclic systems is currently fungal growth while product residues were an important area of research, not only from the found to be more effective at inhibiting bacterial point of view of basic research, but also because growth compared to penicillin G and gentamicin. of the broadening of practical applications of A simple and efficient method has been these compounds. Indeed, they are of great developed for the synthesis of chromenes and importance because of the activity of some of their analogues with better yields under them on living organisms and 68% of drugs are microwave irradiation compared to conventional actually heterocycles. Several methods have been heating. The synthesized products gave very used to synthesize chromenes and their analogs promising results after the study of their such as the classical synthesis used by Verma and antibacterial and antifungal activity. These al For the synthesis of chromene derivatives promising results offer the prospect of expanding from β-oxodithioesters with salicylaldehyde in this study on a variety of active methylene the presence of InCl3 as a catalyst, synthesis compounds with keto-enol equilibrium as under microwave irradiation followed by Kidwai synthons to chromenes. As well as the study of et for the synthesis of 2-amino-chromenes using their biological activities in vivo. K2CO3 as a catalyst in water, Or else, the synthesis of chromenes by ultrasound chosen by Keywords: Biological activitie; Characterization ; Datta and Pasha for obtaining 2-amino-4H- Heterocyclic ; Synthesis. chromenes according to a Michael reaction in very short times and with very good yields. In this work, we synthesized and characterized chromenes and their analogs according to two heating methods with the aim of a comparative 140 Oran 05-07 December

P164 spectrometry (GC/MS) analyses. The production of the enzyme alkane hydroxylase involved with Biodegradation of complex hydrocarbons in aromatic hydrocarbons biodegradation process used engine oil by Gram negative bacteria was verified by polymerase chain reaction (PCR) isolated from Oran harbor for the presence of the alkane hydroxylase gene (alkB). The water harbour isolate Acenitobacter NIL S1*. ABI AYAD S.M E-A1 baumanii, tested up to 15 days, could grow well using used engine oil 1% as sole carbon source. 1 Laboratory of Aquaculture and Bioremediation In the biodegradation analysis, the total viable (AQUABIOR), Department of Biotechnology, University Oran1, Ahmed Ben Bella, B.P 1524 ,El M'Naouer -31000 Oran, count increased to 8.67 ± 2 108 CFU mL-1 on the Algeria. 6th day. The comparison of the chromatogram of [email protected] used oil before and after treatment with the strain, shows that it could degrade several compounds of used engine oil. This strain is able Abstract to grow in the presence of used engine oil Mixture of complex hydrocarbons which include because it has the gene coding for the alkane used engine motor oil and its residues reach the hydroxylase enzyme (alkB). Data obtained give marine water and sediments in different modes, important results in order to utilize these isolate which include deposition from municipal and native harbour microorganisms in the studies of industrial effluents, wastes burn out, accidental bioremediation process. oil spill during transportation or deposits induced by sediment drilling. Used motor oil, Keywords: alkane hydroxylase; GC/MS; harbour contains metals and heavy polycyclic aromatic Oran; used motor oil. hydrocarbons that could contribute to chronic hazards including mutagenicity and carcinogenicity. Prolonged exposure to high oil P165 concentration may increase risk of cancer. Bioremediation can be an alternative green Genotypical identification and determination technology for remediation of such hydrocarbon- of technological properties of lactic acid contaminated surface. The isolation and bacteria isolated from raw camel's milk subsequent identification of native water harbour microorganisms could provide relevant Saidi Yasmine1, Senouci Djamel-Eddine1, Kihal candidate taxa for possible exploitation in future Mebrouk1 biotechnological applications. The primary aim of this work was to isolate native harbour water 1Laboratoire de Microbiologie Appliquée, Département de microorganisms which could be later used in the biologie, Faculté des Sciences de la nature et de la vie, studies of bioremediation (biostimulation) of Université d’Oran1, Es-Sénia Oran, 31100 Algérie. [email protected] harbour waters and assess their potential to oil degrade. Bacterial strains capable to degrade used engine oil have been isolated from samples Abstract of Oran harbour water. Phenotypic In Algeria arid regions, camel milk is considered characterization and phylogenetic analysis of 16S as one of the most important source of dairy rRNA gene sequences of the strain HAR17 products for human diet with potential showed that this isolate was related to members therapeutic effects. Lactic Acid Bacteria (LAB) of the Acenitobacter genus. Spent engine oil are generally associated to habitats rich in degradation potential of this strain was nutrients like many food products (milk, meat, evaluated in Bushnell Haas (BH) medium vegetables, drinks). Recent studies showed that supplemented with used engine oil (1%). The camel's milk is a natural source for probiotics. microbial growth was estimated, every 3 days by The aim of the study was then to isolate, from the measure of UFC/ml, during a period of 15 raw camel's milk, bacteria showing similar traits days. The degradation of several compounds and to LAB, to identify them using genotypic method the metabolites formed during the microbial and to study some of their technologically oxidation process of spent engine oil were important properties. Microbiological diversity confirmed by Gas chromatography–mass

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of Algerian raw camel's milk was determined by interesting and desirable technological abilities phenotypical, physiological, biochemical and such as proteolytic activity, production of acetoin genotypic characteristics. Biodiversity of 41 responsible of flavoring of fermented food and lactic acid bacteria isolated from 12 Algerian raw production of dextran involved in the natural camels milk samples, was determined by means texturing of dairy products. Further studies of microbiological, biochemical, and genetic concerning the safety aspects of these isolates methods. Based on their shape and fermented should be done before using them as starter pattern, 21 isolates, shaped as cocci and culture in dairy industry. homofermentative, were identified as presumptively belonging to the genus Keywords: raw camel's milk, lactic acid bacteria, Enterococcus; 7 heterofermentative coccobacilli genotypic identification, technological traits isolates were considered as Leuconostoc and the rods forms were assigned to Lactobacillus genus (7 isolates). The molecular identification by P166 sequencing the 16S rRNA and superoxide dismutase genes allowed us to identify them as Baseline risk factors that predict the Enterococcus faecium (19 isolates), Enterococcus development of Age-related macular hirae (2 isolates), Leuconostoc mesenteroides (7 degeneration in a sample of the population of isolates) and Lactobacillus rhamnosus (7 the east of Algeria isolates). The technological traits have been examined and the aptitude of each isolates, have Ghania ABID1,2,3*, Mohammed Harmel4, Mohammed been determined. Almost all of the isolates were Missouri4, Iness Dawaji5, Aicha Idder, Nawel Adda able to use citrate as source of carbon to grow in Neggaz6, Wafaa Metteouki1,2,3 , Mostafa Fodil1,2,3, the medium and to produce acetoin. These two Ahlem Messal, Faouzia Zemani-Fodil1,2,3 traits are desirable characteristics since its 1 Universty of Science and technology Mohamed Boudhiaf metabolism produces aromatic compounds such Oran, Algeria. as diacetyl, acetoin and butanediol essential in 2 Laboratory of molecular and cellular biology. dairy industry. Concerning dextran production 3 Thematic Agency for Researcin Health Sciences ATRSS. 4 only Leuconostoc isolates were able to produce it. Departement of Ophtalmology, Djilali Abk Hospital, Sidi Belabess, Algeria. The production of such substancehas to be taken 5 « Hammou Boutlilis » ophtalmology Hospital, Oran, Algeria. into account as an important feature for LAB 6 « Chiali » Ophtalmology clinic, Oran, Algeria. used in dairy environments not only by 7 Hight school in biological science, Oran, Algeria improving their appearance, stability and [email protected] rheological properties, but also by their potentially beneficial properties for human health for example, as an antitumoural Abstract substance. Proteolysis is a critical process for Age-related macular degeneration (AMD) is the LAB to grow in milk, producing organic acids, main cause of blindness and vision loss in old mainly lactic acid, peptides and aromatic people. The development of the disease is a compounds essential in dairy fermentation. In complex interplay of age, environmental, genetic, our study the best result was given by and metabolic and many other factors. The Lactococcus lactis isolates gave the best result. In association between these factors and AMD in other work, lactococci isolates are found to be the population of Algeria is still unclear. The goal frequently used as starter cultures thanks to of this study is to determine whether their high proteolytic activity.The potential environmental factors are risk factors for AMD in production of antimicrobial substances by the a sample of the population of the east of Algeria. camel´s milk LAB isolated in this work was A questionnaire was distributed to all patients assayed by testing the isolates against a variety presenting to Hassani Abk Hospital, Sidi of indicator strains. Enterococcus faecium Belabess, Algeria. Patients were asked to sign an isolates showed interesting results, inhibiting informed consent form and were divided into various indicator strains. In conclusion, Algerian two groups: cases with neovascular AMD, in at raw camel's milk could be considered as a good least one eye, which were diagnosed by the source of new strains of LAB to be used in the attending ophthalmologist, and controls free of production of fermented dairy food by showing systemic and ocular conditions associated with

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AMD. Univariate analyses were performed using combination on RCC causedbya high dose Chi-square test (χ2) for categorical variables and offluoride administration as a strongest oxidant t tests for continuous variables if the normality in Wistar albinos Rats. To reach our objective, assumption was satisfied. Crude odds ratio (OR) twelve female albino Wistar rats were randomly and 95% confidence intervals (95% CI) were divided into 3 groups (n=4); the first group used calculated to determine whether any exposure as a control group. Group II exposed to fluoride factor was significantly associated with AMD. as fluoride sodium (NaF) in drinking water (400 Analyses were done by using SPSS, version 21. ppm) for 70 days. Group III rats exposed to Twenty cases and fifty controls participated in fluoride and treated with Omega 3-Vitamin E the study. Groups were similar with respect to association in feedas (1.825g Eicosapentaenoic sex and age. Univariate analyses found a acid (EPA)+1.21g docosahexaenoic acid (DHA)+ significant association between AMD and alcool 83.3 mg Vitamin E /kg) of dietfor 15 days. The (OR=5.385, P value=0.019), sun exposure histological examination of the cellular structure (p=0.041), and goiter (OR= 3,857 [1,020-14,581], of the kidney tissue of fluoride administrated P value= 0.038). However, smoking, body mass rats,when compared with the control group, index (BMI), diabetes, high blood pressure and recorded a big changes in the glomerulus and consanguinity were not. Our results on the tubules of kidney tissue, also we observed a clear known risk factors were similar to other renal cell carcinoma (RCC); with a very high published data and different to other. This study significant elevation (P<0.001) in the relative confirmed the association of alcohol, sun kidney weight,also creatinine, Urea and uric acid exposure and goiter in development of the as renal function biochemical biomarker disease in the Algerian population. However it is revealed a significant (P<0.05) increase in interesting to expand the study by increasing the fluoride exposed rats when compared to the number of sample size. control group; however, the administration of Omega3-Vitamin E combination ameliorated the Key words: AMD; risk factors; Algerian histological appearance of kidney tissue and population; univariate analyses. minimized the proliferation of RCC;with a highly significant amelioration (P<0.01) in the relative kidney weight,but no significant (P<0.05) improvingbiochemical biomarkers.Kidney P167 hypertrophy (nephromegaly) and loss of kidney functioncan be interpreted by the appearance of Ameliorative impact of Omega 3 and Vitamin hydronephrosis which occurs when the renal E co-treatment on fluoride induced oxidative collecting system of one or both kidneys becomes stress and renal cell carcinoma in rats dilated from the obstruction of urine outflow. Tumor is one of the hydronephrosiscauses. * Anfal Djouadi , SamirDerouiche Prolonged hydronephrosis destroys the tubules in the cortex resulting in renal parenchymal 1Department of Cellular and Molecular Biology, Faculty of natural sciences and life, El-OuedUniversity. atrophy, scarring, or irreversible renal damage. [email protected] This condition progressively leads to the loss of renal function. Histological renal alterations and

RCC refer to damage effects of free radicals Abstract resulting of excess fluoride becauseF-is known to induce free radical generation and consequently Renal cell carcinoma (RCC), is the commonest results in oxidative stress [6]and the assessment and most lethal adults urological cancer forming of oxidative stress parameters confirmed our 3.79% of all malignancies in adults. The interpretation. The findings showed that fluoride incidence and mortality of RCC is increasing in affected antioxidant defense system by developing countries while plateaued in Europe decreasing Glutathione (GSH) level and and North American in the past few years.The increasing malondialdehyde (MDA) level in link between all the risk factors mentioned in kidney tissues with the rate of 55% and 26% study of Al-Bayatiet al (2019) is the free respectively while the GST activity present a non- radicals.We aimed in this study to evaluate the significant variation in fluoride exposed rats beneficial effect of Omega3 and Vitamin E when compared to the control group which

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showed an oxidative stress state.Treated with patients between April 4th to June 16th,2016 at Omega3 and Vitamin E combination improved the Nephrology Department (CHU Mustapha this state, and thereforeamelioratedthe Pasha).We performed the HLA-G assay in the histological appearance of kidney tissue and patient’s sera, using an enzyme minimized of RCC proliferation because of immunoassay(ELISA) which allows us to antioxidant and anti-inflammatory effects visualize an antigen-antibody reaction by means ofOmega3 and Vitamin E. In conclusion, the of a colored reaction produced by the action of present study demonstrated the beneficial effects the enzyme on its chromogenic substrate, this of Omega3 and Vitamin E co-treatment on sandwich reaction based on the use of an anti- histological kidney alterations, oxidative stress HLA-G monoclonal antibody. 2 of the patients and RCC caused by fluoride but this co- tested (with a genoidentical HLA showed a high supplementation not restored some biochemical level of HLA-G,while 2 HLA Haplo-identical markers of renal function, which can be requires presented an average rate, finally 3 a long time of treatment. Further studies are patients(identical haplo or HLA different), are of needed to understand the molecular basis behind HLAG negative. The preliminary results does not thisco-treatment and the necessary time for the make it possible to judge the parameters total remove of renal cell carcinoma. correlated with the expression of this bio- marker, the definitive ones which will be Keywords: Kidney;Omega3-Vitamin E; Oxidative obtained at the end of the study, will make it stress; RCC. possible to consider the use in current practice to adapt and personalize the treatment of transplant patients in Algeria. P168 Keywords: HLA-G; Kidney transplantation; HLA-G as an inhibitor molecule of immune immunosuppressive therapies;tolerance. responses in renal transplantation

Benfattoum Samia1,2,3*,Chebbout Kahina, 2,3 Boudalia P169 Mohamed El Amir2,3 Study of genotypes and alleles distribution of 1Laboratory of Applied Microbiology (LMA), Faculty of Science of Nature and Life ,Univercity of Bejaia,Targua CYP3A5 polymorphisms in the western Ouzemmour, 06000 Bejaia, Algeria. Algerian population: preliminary results 2Immunology department, Mustapha Pacha Teaching Hospital,Place de 1 er Mai 1945, Sidi M’Hamed , Algiers, Amina Ammour1, Abdallah Boudjema1, Sonia 16000, Algeria. Mediene1, Meriem Aberkane1,2 3Nephrology Service, Mustapha Pacha Teaching Hospital,

Place du 1er Mai 1945, Sidi M’hamed, Algiers, 16000, Algeria. 1Laboratoire de génétique moléculaire et cellulaire, Université [email protected] des sciences et de la technologie d’Oran Mohamed Boudiaf (USTO-MB), Oran, Algeria. 2Département de pharmacie Université d’Oran 1Oran Algeria. Abstract [email protected] Kidney transplantation has become the Abstract treatment of choice for end stage renal disease. Cytochrome P450 3A5 (CYP3A5) enzymes are However, despite the considerable progress regarded as an important pharmacogenetics inboth surgical techniques and the use of marker in the development of the personalized immunosuppressive therapies, organ rejection medicine due to the enzymes impact on the remains a major obstacle in kidney efficacy of drugs based on genetic background of transplantation. This study aims first of allto individuals or populations. The aim of our study show the implication of HLA-G in the tolerance of is to explore genetic polymorphisms of the renal transplantation in particular, but also to CYP3A5*3 (rs776746) in a west Algerian categorize patients according to their immune population and compare these genotypes and status with respect to their HLA-G expression, in allelic distributions among different ethnicities. order to consider a personalized prescription for The genotype distribution and frequencies for the reduction of immunosuppressive doses in the c.6986A>G SNP of the CYP3A5 gene were these same patients. We followed 7 transplant

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determined in 143 healthy subjects from the study was carried in the TiziOuzou region, in a Western Algerian population. Genotyping was field of Solanum tuberosum, consists of performed by the Taq Man allelic discrimination determining the diversity and abundance of assay. The genotype frequency of mutant aphids. The study plot was divided into 9 blocks. homozygote CYP3A5*3 /*3 was 62% (n=89), 5% In the middle of each block was placed a yellow (n=8) for the wild type CYP3A5*1/*1 and 39% trap filled with water for the purpose of trapping (n=46) for heterozygote type CYP3A51*/3*. The winged aphids during their flight. In each block distribution of allele frequencies was 78% of the plot, one plant was selected, and the aphids concerning CYP3A5 *3(G) and 21% for CYP3A5*1 found on the potato leaves were collected and (A). The results showed that minor allele preserved in 70% ethanol, for later identification. frequencies (MAF) in our population were In total, 1571 individuals were trapped and similar to those of the Africans populations, as in identified. 55 species divided into 32 genera, 6 Sudan and Morocco, whereas there were distinct tribes and 5 sub-families were enumerated. The differences with some Asians populations as quantitative analysis of the data shows that Japanese and Chinese populations. The size of the Myzuspersicae is the dominant species, this can sample must be increased to confirm these be explained by the presence of its primary and preliminary results. Also, it seems important for secondary hosts. The method of visual counting a complete study to explore other of the aphidian populations installed on the polymorphisms on other genes implicated in foliage made it possible to highlight the presence drugs polymorphisms to enhance the of 3 wingless species: Aphis gossypii, characterization of pharmacogenetics profiles in Myzuspercisae and Macrosiphum euphorbiae with the Algerian population. the predominance of the latter. According to, these three species are characteristic of this Keywords: CYP3A5; genetic polymorphism; culture. M. persicae is reported in Sétif. In the pharmacogenetics; west Algerian. region of Guellal in Biskra, note the presence of M. euphorbiae and M. persicae on a potato crop. P170 During this study, 55 species were captured using yellow traps. The diversity of the species Biology and diversity of potato aphids in Tizi identified is related to the weeds present in the Ouzou region study plot as well as the nearby fruit plants and trees. Three aphid species grow on potato leaves Ait Amar Samia*, Benoufella-Kitous Karima. and can therefore cause damage and loss to this crop. Laboratory of Plant Production, Improvement and Protection Laboratory. Department of Animal and Plant Biology. Faculty Keywords: aphid; biology; diversity; potato. of Biological Sciences and Agricultural Sciences. MouloudMammeriUniversity of Tizi-Ouzou, B.P N° 17 RP 15000,Tizi-Ouzou, Algeria. [email protected] P171

Evaluation of the antibacterial activity of Abstract Artémisia campustris L Potato cultivation is highly susceptible to pest attacks, notably, aphids which are the group of Benchohra Amel Hadria1*, Medjaher Halima 2 3 insects cause the most problems for this crop. Essaadia Souhila , Tounsi Mohamed

Aphids are very common, more than 4700 aphid 1, 2,3, Laboratoire d’écodéveloppement des espaces. species are reported in the world, approximately (faculté des sciences de la nature et de la vie, université de 450 species are identified as pests of cultivated Djilali Liabes , Sidi bel Abbes, 22000 ). plants. Aphids cause damage and reduce [email protected] agricultural yields in several ways. They can build to high population densities, removing plant nutrients, and may damage plants by Abstract removing enough sap to cause withering and Several researches have confirmed the death.The most serious problem posed by aphids pharmacological efficiency of médicinal plants is the vectoring of plant viruses. The present that grow in arid zone, Artémisia campustris L is a

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plant that grows in El bayadh region (Western method of ref. The phytochemical tests of the Algeria) and used in traditionaltherapy. The various extracts are carried out according to the present work deals with the antibacterial protocols described byref. The sensitivity test of activities and phytochemical stadies of ethanolic bacteria and fungi is performed by the diffusion extract. The antibacterial activities of the extract method (the disk method). Phytochemistry was evaluated by the diffusion method in solid screening allowed to highlight the presence of medium on bacteria: Pseudomonas aeruginosa substances of high therapeutic value (flavonoids, ATCC 270853, Staphylococcus aureus ATCC tannins, coumarins, Anthocyanins, 43300, and Escherichia coli ATCC 25922. The Alkaloids…..).The antimicrobial activity of the extract was chromatographied on thin silica gel extracts of the taxa selectedfrom the mount of and observed at 254 nm in the darkroom. The Tessala against three bacterial strains inhibition diameters vary between 11 to 21 mm (Escherichia coli ATTC 25922, Bacillus cereus for Pseudomonas aeruginosa, and 11to 14 for ATTC 10876 and Proteus mirabilis ATTC 35659) Staphylococcus aureus, and 12 to 22 for and two fungal strains (Candida albicans ATTC Escherichia coli. The qualitative analyses realized 10231 and Aspergillus brasiliensis ATTC 16404) by thin layer chromatography (TLC) shows the showed a moderate antibacterial effect on presence of coumarins and flavonoïdes which bacteria and a remarkable activity especially may be responsible for antimicrobial power of against C. albicans. The antimicrobial potential of this plant. In light of the results obtained, it the extracts of the leaves of three studied varied appears that the extract exert antibacterial effect. depending on the taxa, on the tested extract and This effect depends on the concentrations. We its concentration and on the type of microbial plan to complete this work by identify other strain.The extracts produces areas of inhibition compounds that are responsible for the with diameters between 7 and 19 mm, which antimicrobial potency. indicates sensitivity rates varying between 8,33 and 66,67% in the microbial strains studied. Keywords: Artémisia campustris L, Ethanolique These findings have brought additional data for extract, Antibacterial activity, Chromatography, the better knowledge of these taxa especially highlight the differences between these Lamiaceae. These results confirm the biological P172 powers ofthese species. These Lamiaceae of western Algeria and surely the other taxa of the Phytochemical screening and antimicrobial same family of our country contain a lotof active activity of extracts of Phlomiscrinita Cav., substances, which confirms the necessity to Mentha pulegiumL. and Origanum vulgare L. explore these ways for their use in applied herbal from the mount of Tessala (Western Algeria) medicine.

Asma TAMERT1*, Keltoum DERMECHE2 Keywords: Antimicrobial; extracts ; Lamiaceae; Screening. 1 Department of biology, Institute of Science of nature and the life, University ofRelizane, Algeria. 2Department of biology, Institute of Science of nature and the life, University ofRelizane, Algeria. [email protected]

Abstract

The aims of the present study were to evaluate the phytochemical screening of several secondary metabolites and the antimicrobial activities of the ethanolic, decoction and infused extracts of PhlomiscrinitaCav., Mentha pulegium L. and Origanum vulgare L. These species of Lamiaceaeare used intraditional medicine and pharmacopeia. The ethanolic, decoction and infused extracts were prepared according to the

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P173 P174

Genetic relationships and structuration of six The relationship between sweet foods and African local chicken populations non-alcoholic fatty liver disease in type 2 diabetics Fatima Zohra Mahammi1,2*, Semir Bechir Suheil Gaouar3, Michèle Tixier-Boichard4, Nadhira Saidi- Nassima Brixi Gormat-Benmansour1,2*, Fatima Mehtar2, NaceraTabet-Aoul5. Zahra Mohammed Aggad2, Reham Benarbia2, Salima Boukli Hassan3, Meriem Belarbi1. Ecole Supérieure en Sciences Biologique d'Oran, Oran - ALGERIE-. 1 Laboratoire des Produits Naturels, Faculté des Sciences de la Laboratoire de Génétique Moléculaire et Cellulaire, Université Nature et de la Vie et Sciences de la Terre et de l’Univers. des Sciences et de la Technologie d’Oran–Mohamed Boudiaf, Université Aboubekr Belkaid Tlemcen 13000 Oran, Algérie. 2Centre Universitaire Belhadj Bouchaib Ain Département de biologie, Université de Tlemcen, Algérie. Témouchent.Route de Sidi Bel AbessN 101, 46000. Génétique Animale et Biologie Intégrative, INRA, UMR1313, 3 Médecin Endocrinologue Diabétologue à la wilaya d’Ain Jouy-en-Josas, France. Témouchent 46000. Département de Biotechnologie, Université d’Oran Es-Senia, [email protected]

Oran, Algérie. [email protected] Abstract Abstract Excessive consumption of sugary foods has been associated with an increased prevalence of Local chickens represent an important animal metabolic diseases and there is growing evidence genetic resource for improving farmers’ income that it may also contribute to the development in Africa. The present study provides a and severity of non-alcoholic fatty liver disease comparative analysis of the genetic diversity of (NAFLD), a chronic, often silent, it is village chickens across a subset of African characterized by the accumulation of countries. The genotypes of six African local triglycerides in hepatocytes. The aim of our work chicken populations (Algeria, Morocco, is to conduct a retro-prospective study on the Cameroon, Benin, Ghana and Côte d'Ivoire) for relationship between the consumption of sugary 22 microsatellite markers were used to estimate products and non-alcoholic fatty liver disease in genetic diversity within and between these type 2 diabetics. We recruited 40 type 2 diabetics populations. High levels of heterozygosity, residing in the wilaya of Ain Témouchent with an ranging from 0.56 to 0.65, were reported for all average age of 57 years divided into two groups, local populations. The allelic richness varied 20 diabetics with NAFLD and 20 diabetic from 5.68 to 6.35. Very low values of controls, not affected, which we realized firstly, differentiation and genetic distance were anthropometric measurements and establishes a observed between the populations of Benin, questionnaire on their eating habits. Then, we Ghana and Côte d'Ivoire; and this was translated carried out a biological assessment including by the formation of a single cluster on the blood parameters (blood glucose and lipid phylogenetic tree. This result is explained by the balance). The results of the dietary survey show proximity between the three countries and the that the proportion of daily caloric and high level of commercial exchange between carbohydrate intake is higher in steatotic them. The same result was observed between diabetics compared to non-steatosic diabetics, Algerian and Moroccan populations who formed since sweetened products are frequently a second cluster that also included the Cameroon consumed in NAFLD diabetics. In contrast, a population. A more in-depth study of genetic significant disturbance in the lipid profile was differentiation among African populations, taking observed in diabetic steratosics. Our results into account climatic conditions, agricultural implicate the excessive consumption of added systems and cultural practices is needed. The sugars as a risk factor for the development of result will enable us to study the influence of non-alcoholic fatty liver disease in type 2 these parameters on the genetic diversity of local diabetics. chickens. Keywords: Genetic distance, genetic diversity, Key words: added sugars; diabetes Type 2; local chickens, microsatellites. NAFLD; sweet products.

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P175 environmental scanning behavior that reflects the onset of stress, anxiety and depression. This Preliminary study on the probiotic effect of intoxication also revealed a disruption of the Lactobacillus plantarum NSC5C strain on various biochemical parameters tested, in neurobehavioral disorders and oxidative particular the biomarkers of cerebral stress stress induced by lead intoxication in Wistar (MDA). In addition, probiotic treatment resulted rats in decreased blood glucose and MDA rate in brain tissue, decreased body weight, and Sabrina Amara1,2, Mohamed Merzoug1,3, Fatma Imane increased brain weight in young rats compared Laouinati2, Nour-Eddine Karam1 to intoxicated animals. In addition, the use of the various behavioral test techniques reveals that 1 Laboratoire de Biologie des Microorganismes et the probiotic corrects the depressive state, Biotechnologie, Université d’Oran 1 Ahmed Ben Bella, BP1524, Oran El Mnaouer, 31000 Oran, Algeria. anxiety and locomotor hypoactivity observed in 2 Département de Biologie, Université de Saïda Dr. Moulay the rats exposed to Lead. The action of Tahar, Saîda 20000, Algeria. Lactobacillus plantarum NSC5C strain showed a Ecole Supérieure en Sciences Biologiques d’Oran (ESSBO). BP protective effect against depression and anxiety as 1042 Saim Mohamed (EX-IAP) 31003 Oran. well as on the oxidative stress induced by lead [email protected] intoxication, it would be interesting to deepen this line of research by measuring more biomarkers of Abstract oxidative stress on brain and liver and compare the efficacy of NSC5C strain against other Lead is a metal that is ubiquitous in the probiotics and antioxidant substances. Safety of environment and has a well-established toxicity; the strain should also be checked before it is considered a non-essential element for the considering clinical studies. vital functions of the body. Exposure to this metal, even at very low doses, causes significant Keywords: Lead; Neurobehavioral disorders; dysfunctions in various organs, particularly the Depression; Anxiety; Probiotics; Lactobacillus central nervous system, which can lead to plantarum; Oxidative stress. neurobehavioral disorders. To this end, our work focuses on the impact of lead intoxication on anxiety, depression and oxidative stress in vivo P176 and the ability of the probiotic strain Lactobacillus plantarum NSC5C to counter Phytochemical study and biological activity of squeals generated by this metal. The experiment a medicinal plant "Cotula cinerea" of the was conducted for 7 weeks on Wistar rats. South-West of Algeria Animals were divided into 3 batches (a non- intoxicated control batch, an untreated Bettayeb Zouaouia, Mohammed El-Amin Said, Toumi intoxicated batch, and an intoxicated batch Fawzia, Benyamina Abdelfetah receiving probiotics as a dietary supplement). Daily intoxication is carried out orally by diluting ¹University of Djilali Liabes Sidi Bel-Abbes, Laboratory of Ecodevelopment Spaces, BP 89, 22000 Sidi BelAbbes, Algeria. 2 g/l of lead acetate in the drinking water; the [email protected] probiotic strain is presented in the form of fermented milk diluted at 10% in the water presented to the treated batch. At the end of the Abstract 7th week of study, behavioral tests indicative of In the context of the plant valorization, the depression and anxiety are carried out, present work is interested in the phytochemical specifically tests of forced swimming, labyrinth of study and the antioxidant activity of essential oil raised cross and open field. The blood and the of the flowers of a medicinal plant from the brain are taken after the sacrifice of rats to assay South-west Algerian (Cotula cinerea, El guertoufa respectively glycemia and malondialdehyde lbayda) which is a specie belonging to the family (MDA) which represent one of oxidative stress Asteraceae. The essential oil was extracted by biomarkers. Results show that daily exposure to hydrodistillation using a Clevenger type lead causes neurobehavioral abnormalities apparatus. Then, the antioxidant activity of this resulting in locomotor hypoactivity and reduced oil was evaluated by the two methods which are

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the trapping of the radical DPPH and the metabolic diseases. Indeed, each unit increase in bleaching of ß-carotene. Thus, the study of the the body mass index (BMI) increases the risk of chemical composition is done by the GC-SM hypertension by a factor of 5 and the risks of technique. The results of the antioxidant activity coronary artery disease and stroke by a factor of reveal that the essential oil of the flowers has no 3.6. It is noteworthy that 80% of people with inhibitory effect of the DPPH radical in type 2 diabetes are obese. We sought to establish comparison with the standards used (ascorbic whether genetic variants that are robustly acid, gallic acid and catechin). On the other hand, associated with BMI could modulate our oil has shown considerable antioxidant anthropometric traits and the obesity risk in an power by preserving the color of ß-carotene in a Algerian population sample, the ISOR study. The manner similar to that of catechin and gallic acid. ISOR study of 787 adult subjects (aged between As regards the chemical composition, the 30 and 64) provided a representative sample of essential oil components of flowers are ß- the population living in the city of Oran (north- thujone; santolinatriene and camphor. These west of Algeria). We investigated the combined results suggest that our species could serve as an effect of 29 BMI established genetic variants alternative source of active ingredients using a genetic predisposition score (GPS) on producing pharmacological activity. anthropometric traits and obesity risk in 740 subjects. We found that each additional risk allele Keywords: Algerian Cotula cinerea, essential oil, in the GPS was associated with an increment in antioxidant activity, chemical composition. the mean [95% CI] for BMI of 0.15 [0.06 - 0.24] kg/m² (p = 0.001). Although the GPS was also associated with higher waist (p = 0.02) and hip (p = 0.02) circumferences, these associations were P177 in fact driven by BMI. The GPS was also associated with an 11% higher risk of obesity Combined effect of established BMI loci on (OR [95%CI] = 1.11 [1.05 - 1.18], p = 0.0004). We obesity-related traits in an Algerian also observed that few SNPs presented population sample significant allele frequency differences between the ISOR and Speliotes et al. studies. Differences 1 1,2 Badsi Manel Nassima , Mediene-Benchekor Sounnia , in allele frequencies may contribute to Ouhaibi-Djellouli Hadjira1,2, Lardjam-Hetraf Sarah differences in disease prevalence between ethnic Aicha1, Boulenouar Houssam1, Meroufel Djabaria Naïma1, Hermant Xavier3, Hamani-Medjaoui Imane4, groups. The overall known genetic susceptibility Boudjema Abdallah1, Amouyel Philippe3, Houti associated with the GPS explained only 1.0% of Leila1,5,6, Meirhaeghe Aline3, Goumidi Louisa3. the variance in BMI, whereas the combined effect of genetic and known environmental factors 1Laboratoire de Génétique Moléculaire et Cellulaire, accounted for 14.1%. Our data showed that a GPS Université des Sciences et de Technologie d’Oran Mohamed comprising 29 BMI established loci developed Boudiaf, BP 1505 El M’Naouar, 31036 Oran, Algeria. 2Département de Biotechnologie, Faculté des Sciences de la from Europeans seems to be a valid score in a Nature et de la Vie, Université d’Oran, BP 1524. El M’Naouar, North African population. Our findings 31000 Oran, Algeria. contribute to a better understanding of the 3INSERM, U744; Institut Pasteur de Lille, Université Lille Nord genetic susceptibility to obesity in Algeria. de France, 1 rue du Pr. Calmette, BP 245, F-59019, Lille Cedex, France. 4CNAS Hai Bouamama (El Hassi), Caisse Nationale des Keywords: Genetic predisposition score, Assurances Sociales des travailleurs salariés, Clinique Polymorphism, BMI, Obesity, Algerian Spécialisée en 5Orthopédie et Rééducation des Victimes des population, ISOR study. Accidents de Travail, Oran, Algeria. 6Faculté de Médecine, Université Djillali Liabes de Sidi Bel Abbès, BP 89, 22000, Sidi-Bel-Abbès, Algeria. 7LABoratoire des Systèmes d’Information en Santé, Université d’Oran, BP 1524 El M’Naouar, 31000 Oran, Algeria. [email protected]

Abstract Obesity (as characterized by excess body fat) is an established risk factor for cardiovascular and

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P178 ergokryptinine,ergocroninine and ergosinine) in cereal samples. The analytes were extracted Multi-mycotoxin occurrence and risk using an acetonitrile and ammonium carbonate characterization in foodstuffs from Algeria solution, followed by purification with C-18 and Z + sep sorbent. A simple method for the Khelifa Mahdjoubi Choukri (1,2,4)*, Arroyo- determination of aflatoxin B1, B2, G1, G2 and Manzanares Natalia (3), Hamini Nisserine (2), García- ochratoxin A in cereals based-product using (1) (4) Campaña Ana Maria , Haidukowski Miriam , immunoaffinity column extraction in (2) (1) Mebrouk Kihel , Gámiz-Gracia Laura , Logrieco combination with quantification with UHPLC. Antonio(4) Analytical results showed that 99 cereal samples

1Dept. Analytical Chemistry, Faculty of Sciences, University of (52%) were contaminated with at least one toxin, Granada, Spain while 36% of the samples were contaminated 2Dept. Biology, Faculty of Natural and Life Science, University with two to nine mycotoxins. Moreover, aflatoxin of Oran 1, Algeria G1, T-2, citrinin, beauvericin and deoxynivalenol 3Dept. Analytical Chemistry, Faculty of Chemistry, University of Murcia, Murcia, Spain were the most commonly found mycotoxins with 4Italian National Research Council (CNR), Bari, Italy a frequency of 41.6%, 36,4%, 25.8%, 23.5% and 20.9%, respectively. Fumonisins, enniatins B and B1, deoxynivalenol and zearalenone registered Abstract high concentrations, ranging from 4-48878 Mycotoxins are toxic compounds produced by µg/kg, 1.2-5288 µg/kg, 15-4569 µg/kg, 48-2055 different types of fungus, belonging mainly to the µg/kg and 10.4-579 µg/kg, respectively. Aspergillus, Penicillium, Fusarium and Claviceps Furthermore, mycotoxin concentrations higher genera. European Union and many others than those allowed by the European Union were countries had established maximum levels for observed in 27,17 6, 2 and 1 samples for those mycotoxins considered as chemical fumonisins, the sum of aflatoxins, zearalenone, hazards in Regulation and subsequent ochratoxin A and deoxynivalenol. The risk amendments. However, other countries such as exposure assessment concluded that the high Algeria have not established any regulation levels found for fumonisins (FB1+FB2) in maize concerning those contaminants, and scarce and for DON, ZEA and the sum of (HT-2+T-2) in controls on the products susceptible of wheat, represent a high health risk for the contamination are carried out, with the average adult consumers in Algeria. The obtained subsequent risk for the consumers.The aim of results pointed out the necessity for a consistent this study was to evaluate the mycotoxin control over these contaminants and the contamination of 190 samples of different cereals definition of their maximum allowed levels in (maize, rice, wheat and barley) and cereals different foodstuffs. Hence, more data about based-product (couscous and semoulina of these mycotoxins with further works with higher barley) obtained from Algerian markets. number of samples and other susceptible food Subsequently the risk exposure of Algerian products would be needed to assess the population over the consumption of these situation. On the other hand, the focus should foodstuffs was evaluated. A method based on a also be directed towards reduction and control of QuEChERS-based extraction and ultra-high mycotoxins in the food chain. performance liquid chromatography coupled to Keywords: Mycotoxins, liquide chromatography, tandem mass spectrometry (UHPLC–MS/MS) risk assessment, Algeria. was developed and validated for the analysis of (aflatoxin B1, B2, G1 and G2, ochratoxin A, deoxynivalenol, zearalenone, fumonisin B1 and B2, T-2, HT-2 toxin, fusarenon-X, citrinin, sterigmatocystin, beauvericin, enniatin A, A1, B and B1) in cereals. An UHPLC–MS/MS method was developed and validated for the simultaneous determination of 12 ergot alkaloids (ergotamine, egometrine, egocristine , ergokryptine,ergocronine, ergosine , ergotaminine, egometrinine, egocristinine,

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P179 revealed remarkable antioxydant capacities; this essential oil is active with IC50 (concentration Antioxydant Activity of the Essential Oil of inhibiting 50% of DPPH) of about 272.52 μg / ml Sature jacandidissima (Munby) Briq. Native and EC50 (the median effective concentration to Plant from West Algeria reduce iron) of 28.89 mg / ml. The total antioxidant capacity (CAT) was very important Amina ATTOU 1*, Dominique DAVENNE2, and it exceeds 329 mg equivalent of ascorbic acid HamadiAbderrahmane LAZOUNI 1, Fatima KHOLKHAL per gram of essential oil. 1, Mohamed AMARA3

1 : Laboratory of Natural Products: biological activities and Keywords: Essential oil, Sature jacandidissima synthesis, Department of Biology, Faculty of Sciences, (Munby.) Briq., GC/MS, Antioxydant activity. University AbouBekrBelkaid, P.B.119 Tlemcen, 13000, Algeria. 2: University of Rennes, Faculty of Pharmacy, Departement of Pharmacognosy, 35043, France/ Laboratory Rosier Davenne, Campany Specialized in Essential Oils, Avignon, 84140, P180 France. 3 : Laboratory of Ecology and Management of Natural Ecosystems. University AbouBekrBelkaid, Tlemcen, Algeria Assessement of the knowledge of pharmacists [email protected] community on oral anticancer therapy in the treatement of breast cancer Abstract: Hania Rim BAHMED

For many years, a variety of different chemical Pharmacy department, TALEB MOURAD medical school, and synthetic compounds has been used as University of DJILLALI Liabes, Sidi Bel Abbes, Algeria. antimicrobial agents, but the widespread of their [email protected] use causes ecological and medical problems (Bernadet, 2000). That’s why; there is a renewal interest in the use of herbs and spices that have Abstract been used since ancient times for their perfume, Oral anticancer agents (OAA) have allowed flavor and preservative properties. Among breast cancer patients to take their treatment in natural antimicrobials are the essential oils the comfort of their home. However, this kind of extracted from many plants, and have been therapy brings patients to assume greater reported to possess a wide spectrum of responsibility in the management of their antibacterial activity (Kar, 2007). Sature therapeutics. Pharmacists,who delivred theses jacandidissima (Munby.) Briq. is an aromatic products, can support patients by providing a medicinal plant belonging to family Lamiaceae, therapeutic education of their treatment. The harvested between rocky lawns in west Algeria main aim of our study is to assess the knowledge and still used by locals to flavor culinary dishes of the pharmacists community on oral anticancer and also to treat flu and fever, intestinal worms therapy in the treatment of breast cancer. We and painful periods. It’s called by locals “ Nabta el conducted a cross-sectional study from March beda” or “Zaatercheleuh” (Quezel and Santa, 10th,2016 to March 31st,2016 in 50 pharmacies 1963). Our sample of Saturejacandidissima nearby the Oran University Hospital"CHUO", (Munby.) Briq. from west Algeria (Ain University Hospital EHU 1st November of Oran Temouchent) has been subjected to and the anti-cancer center of Oran(CAC) that hydrodistillation for 3 hours and then analysis treat breast cancer patients taking OAA. The using a GC/MS clarus model. The essential oil survey was realized by using a self-administred yield from the whole aerial parts of questionnaire. The study has showed that 64% of Saturejacandidissima is 0.90% (w/w), it’s an pharmacists thought their knowledge level about important yield compared to other species of OAA was average. 72% said that some questions Satureja genus. Twelve constituents were asked by patients put them in difficulty and 28% identified, representing 90.61% of the total oil felt that their ability to ensure an education and fraction. The major constituents of the oil were to counsel patients on OAA use was low. These pulegone (47.62%), menthone (24.50%) and results has showed the problem of Algerian terpinen-4-ol (11.63%). The evaluation of the pharmacists to ensure the safety of OAA antioxidant activity by three chemical methods, delivrance. Many surveys noticed the same

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concern on the subject : in Japan, a study has P181 established that only 6-10% of pharmacists felt that they had received adequate education in Characterization and immunomodulation of oncology or oral chemotherapy. Only 54% felt exopolysaccharides-producing lactic acid comfortable dispensing OAA and only 40% felt bacteria strains of technological interest comfortable educating patients about oral chemotherapy. In Saudi Arabia, a study has Kenza Zarour 1,2*, Alicia Prieto 2, Patricia Ruas- revealed that about 20% of Pharmacists had Madiedo 3, Mebrouk Kihal 1 and Paloma López 2 adequate knowledge about oral anticancer Department of Biology. University of Oran 1 Ahmed Ben Bella, agents, and only 26% felt somewhat confident to Es Senia. 31100. Oran, Algeria. dispense OAA. In USA,a study in Arkansas has Department of Molecular Microbiology and Infection Biology. pointed out that only 22% of respondents Center for Biological Research, CIB-CSIC. Ramiro de Maeztu 9. reported confidence in their ability to manage an 28040. Madrid, Spain. influx of OAAs, and the majority of respondents Department of Microbiology and Biochemistry of Dairy Products, Institute of Dairy Products. Asturias, Spain (77%) reported that their level of training or *[email protected] knowledge was the main barrier to counseling patients. The main reason of the knowledge gaps among pharmacists could be the limited formal Abstract education about OAA as well as the lack of Lactic acid bacteria belonging to the genus medical training after their graduation. Leuconostoc are Gram positive bacteria with One study performed in Canada has showed that technological interest related to their properties only about 14% of pharmacists felt that they had to: 1) ferment food product matrices; 2) produce adequate oncology education during their carbon dioxide during the production of cheeses; pharmacy school curriculum and that fewer than 3) produce of aroma compounds; 4) produce 20% had attended an educational program exopolysaccharides (EPS), which are used as related to oncology in the past 2 years. Fewer food additives; and 5) probiotic potential of than 10% of them interest for the elaboration of functional foods. felt comfortable educating patients on OAA. To The aim of this study is characterization of resolve this problems, in Germany, Health strains of lactic acid bacteria (LAB) and their programs have been established by the German exopolysaccharides (EPS). Six Leuconostoc Society for Oncology Pharmacy« DGOP » and the mesenteroides strains isolated from camel milk German Cancer Society to develop training and sheep milk were selected according to the programs and to provide tools to support level of production of EPS both in liquid medium community pharmacists interacting with cancer as solid. EPS were produced in the presence of patients taking OAA. Our study has revealed sucrose, purified from bacterial culture knowledge gaps in Algerian pharmacists supernatants by ethanol precipitation, dialyzed community about OAA and low confidence in and lyophilized. The purified EPS and producing dispensing these medications.The need of bacteria were used in serval studies, as well as, specialized university lectures on oncology metabolic study, physicochemical analysis and medications and medical training programs after determination of immunomodulation ability. The graduation is important to improve safety of metabolic study of these strains in the presence delivrance by pharmacists and therapeutic of sucrose showed a correlation between sugar education of patients. consumption and the synthesis of their intermediate (glucose and fructose) and final Keywords: breast cancer; oral anticancer agents; metabolites (lactate and mannitol). The pharmacists. production, precipitation and purification of the exopolysaccharides showed a final yield of the polymers between 1.2 and 3.6 g L-1. Methylation analyzes and the determination of the composition of the monomers revealed that the homopolysaccharides are dextrans with a main chain of glucopyranose units linked by α- (1,6) bonds, and partially branched (between 6.6%

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and 10, 3%) in the O-3 position. Size exclusion Constantine for Alzheimer's disease. The data is chromatography coupled with multiangle laser collected following a questionnaire. All the data light scattering (SEC-MALLS) detection analysis in our study were processed using the Epi Info 7 demonstrated that the dextrans had high software. The results found show that the mean molecular masses which varies between age in AM patients is 71.65 ± 8.82 years, with a 1.74x108 Da and 4.41x108 Da. In addition, mean onset of 68.55 years ± 8.19 years, Our dextrans clearly showed anti-inflammatory results are consistent with those of the literature. immunomodulatory activity in vitro, using THP-1 The sex ratio is 0.7 in favor of the female sex, our macrophages cell line. The LAB and theirs results are consistent with those of Ben Djebara dextrans showed a particular technological and Gouider. The majority of the affected interest that qualified them to be used in the subjects are housewives. Alzheimer's disease has agri-food industry producing functional foods. As become a serious public health problem as it is perspective, the characterization of responsible for about half of the current dextransucrase enzyme, responsible to produce dementia syndromes. A genetic study is in dextrans, is crucial to complete the study of EPS. progress. Keywords: exopolysaccharides; immune- Mots clés: maladie d’Alzheimer; épidemiologie. modulation; lactic acid bacteria; metabolim.

P183 P182 Epidemiological survey of the seroprevalence Alzheimer's disease: epidemiological of toxoplasmosis in pregnant women in the characteristics region of Tizi-Ouzou

Rayene Achou 1*, Dalila Satta 1 , Abdelmadjid Hamri 2. Djouaher Thinhinane1*, Ziane Katia2, Aouar-Sadli Malika3, Mouloua Abdelkamal4, Brahmi Karima5, Ait Laboratoire de biologie moléculaire et cellulaire, Amar Samia6. Département de biologie animale, Faculté des sciences de la nature et de la vie, Université des Frères Mentouri, 1, 2, 4, 5 Laboratory of Ecology and Biology of Terrestrial Constantine , 25000, Algérie. Ecosystems Le BIOT. Drepartement of Animal and plant Service de Neurologie, CHU Ben Badis Constantine, Biology. Faculty of Sciences Biological and sciences Laboratoire de biologie et génétique moléculaire, Université Agronomic. University of Mouloud Mammeri, B.P. N° 17 RP Salah Boubnider, Constantine, 25000, Algérie. 15000 Tizi-Ouzou, Algeria. [email protected] 3, 6 Plant production, improvement and protection laboratory. Departement of Animal and plant Biology. Faculty of Sciences Biological and sciences Agronomic. University of Mouloud Abstract : Mammeri, B.P. N° 17 RP 15000 Tizi-Ouzou, Algeria. [email protected] Alzheimer's disease (AD) is the most common neurodegenerative disease. It affects more than 44 million people worldwide, and is associated Abstract: with atrophy of the cerebral cortex, following the Toxoplasmosis is a cosmopolitan formation of beta amyloid plaques and anthropozoonosis caused by toxoplasma gondii. neurofibrillary tangles. It is characterized by It is often benign in immunocompetent cognitive disorders, temporal-spatial individuals but may be formidable in the disorientation, behavioral disturbances, immunocompromised and when it occurs during executive functions and leads in the end to a loss pregnancy, due to the transmission of the of autonomy. Risk factors are being evaluated parasite to the fetus which exposes it to around the world. Given the lack of scientific congenital toxoplasmosis. The purpose of our work on AM in Algeria, our goal is to establish a study is to estimate the seroprevalence of retrospective study on medical records of AM toxoplasmosis in pregnant women in the region patients. The retrospective descriptive study is of Tizi-Ouzou and see if there is a relationship based on the medical records of 40 patients between age, parity and acquisition of followed in specialized consultation at the toxoplasmic infection. Our study is a cross- University Hospital Center Ben Badis, sectional survey, which took place from February

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25th, 2018 to April 05th, 2018, involving a P184 sample of 355 pregnant women. It was carried out in three places namely: the city of Tizi-Ouzou, Beneficial Effect of Paronychia capitata L. TiziRachid andLarbaaNathIrathen. An aqueous Extract on Ethylene Glycol-Induced information sheet produced for this purpose Urolithiasis in Rats enabled the collection of various epidemiological data. Data interpretation is performed by Y. Abismail1, A. BerrabbahAlioua1, F. Belhoucine1, N. software: Excel and Statistica. The presence of Bouredja1, M. AitKaci1, K. Bentayeb2, O. Kharoubi3 association between two variables is measured 1Department of the Living and the Environment, Faculty of by a test (X2), a value of p<0.05 was considered Sciences of Nature and Life, University of Science and statistically significant. A total of 355 Technology of Oran Mohamed Boudiaf, USTO-MB, PO Box questionnaires were retrieved, but the analysis 1505, El M 'naouer, 31000 Oran, Algeria. was limited to 329 questionnaires for which the 2Department of Chemistry and Environment, Faculty of women’s immune status is known. The Chemistry, University of Sciences and Technology of Oran Mohamed Boudiaf, USTO-MB, PO Box 1505, El M'naouer, seroprevalence of toxoplasmosis in pregnant 31000 Oran, Algeria. women according to our study in the region of 3Laboratory of Biochemistry and Biotoxicology, Experimental, Tizi-Ouzou is 44.98%. Our study revealed a Biodepiration and Phytoremediation (BTE-BD-PR), Faculty higher prevalence than that reported in 2015 in SNV, Department of Biology, University of Oran 1 Ahmed Ben Bella, 31000 Oran, Algeria. the region on Tizi-Ouzou, which was 34.5%. On [email protected] the other hand, it is slightly lower than that obtained in2017 in the region of Tizi-Ouzou which was48.34%. These differences could be Abstract: explained by some improvement in hygiene Herbal plants and their extracts are the best conditions but especially by the nature of the remedy for prevention very different sampling in the studies.In our and cure of urolithiasis as they play roles in study seroprevalence increases with age. Several controlling the process studies have reported an increase in of crystallization events. Paronychia capitata L. seroprevalence with age. In our study parity was known under the name of (Atai el Djebel). It is not identified as a predictor oftoxoplasmic also used as cholagogue, dermatologic, anti- immunization (p=0.07). Our observation is infective, lithotritic, diuretic, digestive and consistent with that made in Agadir and Rabat. antihypertensive.The aim of the current study is So, in our study area, women whether to evaluate the beneficial effect of the aqueous primiparous or multiparous, could have the same extract (decoction) of the plant Paronychia possibilities to be infected with capitata L. as a preventive agent in toxoplasmagondii. The experimentally induced nephrolithiasis in a rat toxoplasmicseroprevalence obtained during our model. Ethylene glycol (EG) was used in the study is 44.98%. This result showed that experiment to induce calcium oxalate (CaOx) pregnant women are highly exposed to deposition into kidneys. In preventive protocol, toxoplasma gondii in the region of Tizi-Ouzou. Paronychiacapitata decoction extract was Today, there is no vaccine to prevent administered in the same day with EG to evaluate toxoplasmosis in humans.So, the compliance with the ability of the extract to prevent crystal dietary and lifestyle measures remains the only deposition. Nephrolithiasis was induced in male prevention available to all non-immunized Wistar rats by adding ethylene glycol (0.75%) in pregnant women. drinking water for 28 days. Animals divided in six groups each containing six animals. Vehicle Keywords: pregnant women; Seroprevalence; control, model control and P. capitata aqueous Tizi-Ouzou; Toxoplasmosis. extract in dose of 500 mg/kg of b. w the rat.The animals were sacrificed under 10% chloral anesthesia at a dose of 3 mg / kg b w of the rat 24 hours after the last dose. After which different dosages were carried out: urinary volume of 24 hours, urinary pH and crystalluria. As well; the urinary concentration of crucial ions contributing to renal stone formation was evaluated: calcium,

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phosphate, uric acid and magnesium in urine. compounds has been shown to be detrimental to The aqueous extract of Paronychia capitata L. human health especially during sensitive periods was tested for its antilithiasis activity. A of life including in utero exposure. This work significant decrease in calculus weight was aims to study metabolic and redox effects on observed after treatment in the animals receiving serum and kidneys, and histological changes of the aqueous extract compared to the control long-term exposure to alpha-cypermethrin (1.5 groups. This extract showed an increase in mg/ kg body weight / day) in offspring of female urinary volume of 24 h compared to the control. Wistar rats, themselves exposed before and Impairment of renal function and oxidative during pregnancyto the same dose, in order to imbalance in kidney observed in calculi induce establish if maternal exposure to alpha-CYP group. Treatment with P. capitata decreases during gestation and lactation affected offspring hyperoxaluria, calcium, uric acid, improves renal metabolism andredox status and kidneys function. Crystalluria was characterized by function in adulthood. Wistar female rats aged 6 excretion of large calcium oxalate crystals in weeks and weighting about 110 ± 20 received lithogenic group but smaller in drug treated before and during gestation the standard diet group. The histology showed depositions of large contaminated or not with alpha-cypermethrinat number of calcium oxalate crystals in kidney in the no observed effect level (NOEL, 1.5 mg / kg / calculi induced group while in the treated group day). This exposure is maintained during small and fewer deposits. This extract showed a lactation and, after weaning, the offspring is 24-hour increase in urinary volume compared to followed until adulthood (3 and 5 months of the control. The diuretic activity observed may postnatal life). Weight and ingested food are help prevent the deposition of oxalate stones. noted, energy intake is calculated. At the end of The present investigation has shown that the the experimental protocol, the rats are sacrificed; plant extract clearly prevents the formation of Serum and kidneys biochemical parametersand urolithiasis. The result indicates antiurolithiatic markers of oxidant/antioxidant status are activity of P. capitata mediated possibly by determined. Histological sections are madein the calcium oxalate crystal inhibition and kidneys to detect possible tissue alteration. Our maintaining balance between stone promoters results show that long-term exposure to alpha- and inhibitors constituents and this study cypermethrin induces, in both sexes, a significant rationalized its medicinal use in urolithiasis. reduction in body weight, food intake and energy Inperspective, further studies are needed to intake; hyperglycemia and increased plasma identify the anti-lithiasis component of this plant. levels of urea, creatinine and uric acid, often accentuated during pre- and post-natal exposure. Keywords: Paronychiacapitata L., ethylene Oxidant / antioxidant status was impaired in glycol, urolithiasis, calcium oxalate, Crystalluria. insecticide-treated rats, with increased plasma, erythrocyte and kidney levels of malondialdehyde and carbonyl proteins, and a significant reduction in catalase activity and P185 reduced glutathione, in males and females at day 90 and day 150, with an accentuation of this Effect of maternal exposure to a pyrethroid on imbalance during prenatal exposure. the renal function of Wistar rat offspring Contamination by type II pyrethroids induces a reduction in body weight. Increased serum levels Haféda Ghorzi1*,Hafida Merzouk1,Leila Hocine1. of creatinine and urea and the impaired Redox Laboratory of Physiology and Biochemistry of Nutrition, status may be due to kidney tissue damage and Department of Biology, Faculty of Natural and Life Sciences, disruption of kidney function.Histological Earth and Universe, University Abou-BekrBelkaïd, Tlemcen changes in renal tissue architecture are marked 13000, Algeria. by the presence of an inflammatory infiltrate and [email protected] cellular degeneration, aggravated by combined pre- and postnatal exposure. Repeated oral Abstract exposure of cypermethrin has considerable Alpha-cypermethrin, a type II pyrethroid harmful effects on body organs, section of kidney insecticide, is widely used throughout the world displayed hemorrhage andsloughing off renal in several domains. Long-term exposure to these epithelial cell in the convoluted tubules, 155 Oran 05-07 December

shrinkage of glomeruli, and necrosis of renal ANN) were built in accordance with the OECD tubules. guidelines including internal, external validation Chronic exposure to alpha-cypermethrin affects and chemical applicability domain (AD). The metabolism, resulting in permanent changes in ANN method, considering the relevant biochemical parameters and redox markers, as descriptors obtained from the MLR. Stability and well as kidney tissue damage. These predictive ability of model ANN is more abnormalities are exacerbated in offspring with significantly that MLR model. The best maternal exposure.Alpha-cypermethrinshould be computational neural network model was with used withcaution especially during pregnancy. It R2=0.96 and MSE = 0.06. Furthermore, Y- isstronglyrecommended to limit the use of scrambling was applied to evaluate the synthetic pesticides and replace themwith bio- possibility of chance correlation of the predictive pesticides in the differentfields, whether model. All validations showed that the ANN agricultural or domestic. model can be used quite satisfactorily for the screening of new series of molecules with anti- Keywords: Alpha-cypermethrin;kidney; AChE activity. Offspring; Oxidative stress. Key-words: - Acetylcholinesterase, ANN, QSAR, Virtual screening. P186

Artificial neural network models based on P187 QSAR for predicting and screening of novel AChE inhibitors as anti- Alzheimer drugs Study of the effects of lemon balm (Melissa officinalis) on long-term memory in Wistar Anfel Zerroug*(1), Imane Almi (1), Salah Belaidi (1) male rats (1)Group of Computational and Pharmaceutical Chemistry, LMCE Laboratory, Manseur Amir, Bairi Abdelmajid, Bakeche Amira,

University of Biskra, Algeria Djouini Amina, Tahraoui Abdelkrim. [email protected] Applied Neuroendocrinology Laboratory, Department of Biology, Faculty of Science, University Badji Mokhtar BP12 23000, Annaba, Algeria. Abstract: [email protected] Acetylcholinesterase (AChE) is an enzyme that catalyzes the breakdown of the neurotransmitter Abstract acetylcholine (Ach) this one is essential for cognition and memory. Since low levels of this This work focuses on the research of effects of signaling molecule are associated with the lemon balm (Melissa officinalis) on long-term development of Alzheimer’s disease (AD) memory, it is a perennial plant of the Lamiaceae characterized by loss of cognition and impaired family, used for centuries in traditional medicine intellectual ability and functionality. The main as an antispasmodic, antiviral, and anti-stress aim of our research was to build a neural model, agent. The active substances of Melissa officinalis which could be used for screening of new anti- are obtained after ethanolic extraction of the AChE inhibitors without need of expensive fresh leaves, the therapeutic effects of our experimental test. The artificial neural network product are then tested on male Wistar rats, (ANN) models were based on a quantitative which receive by oral administration different structure–activity relationship (QSAR) study was concentrations (50, 150, 300 mg / kg) on a investigated in a series of 53 pyridazine period of 10 days. Our rats are then evaluated derivatives acting as AChE inhibitors (AChEIs). using a behavioral device that measures the long- The principal component analysis (PCA) has term memory state (MWM: The Morris water been used to select descriptors that show a high maze). Our results suggest that animals treated correlation with activities. The k-means method with Melissa officinalis have longer-term was used to divide the dataset into training and memory more developed than controls. Oral test sets. The QSAR models developed (MLR and ingestion of Melissa officinalis has positive effects

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on long-term memory. It is important to note that P189 the duration of the administration had a significant impact on the observed behavioral Antioxidant property of hydrolysates derived pattern. from the hydrolysis of goat's milk caseins by Lactobacillus Proteases Keywords: Melissa offcinalis; Wistar; herbal medicine; long-term memory. Y Zater, S Roudj, N.E Karam

¹ Laboratory of Microorganism Biology and Biotechnology, University of Oran1 Ahmad Ben Bella, 31000, Oran, Algeria. P188 [email protected] In vitro chemical and biological evaluation of a forage plant from arid zones Abstract:

Sabrina Lekmine 1,2*, Mostapha Brahmi 5, Samira It is well known that lactic acid bacteria, widely Boussekine 1, Kenza Kadi 3, Ouided Benslama 2, used in the dairy industry, play an important role Hemza Ouaret 4, Amokrane Serine 6, Rabah Arhab 1,2. in the production of peptides derived from milk proteins, which can perform beneficial biological 1 Laboratory of bioactive molecules and applications, activities in addition to their nutritional roles. University Larbi Tébessi, Tebessa, Algeria 2Laboratory of biotechnology of natural substances and These bioactive peptides could be involved in application Faculty SNV, University Larbi Ben M'hidi. Oum El maintaining or improving human health. The Bouaghi objective of our study was to select a proteolytic 3Laboratory Biotechnology, Water, Environment and Health, lactic strain and investigate its ability to generate University Abbes Laghrour, Khenchela, Algeria. 4University of Science and Technology Houari Boumediene. antioxidant peptides. A strain of Lactobacillus 5Laboratory of biotoxicology, pharmacognosy and biological labeled LBBS1 with proteolytic potential has valuation of plants. Univ Dr. Moulay Tahar-Saida been selected by screening on agar-milk medium 6Biotechnology Research Center (C.R.Bt), Ali Mendjeli, New from 14 strains. This strain has shown the ability Town, UV 03 BP E73 Constantine, Algeria to release proteases into the culture medium, [email protected] their activities on caseins from goat's milk have been evaluated by biochemical methods. The Abstract detection of antioxidant activity by trapping the free radical diphenylpicrylhydrazyl (DPPH) of This work aims to evaluate in vitro the activity of the hydrolyzates obtained revealed an saponins extracted from Astragalus gombiformis. antioxidant power. This study showed that It will also focus on the determination of its Lactobacillus proteases are able to hydrolyze nutritional value. In our experimental protocol caseins at cleavage sites leading to peptide we carried out several techniques to determine sequences with antioxidant activity. Antioxidants the mineral salts, dry matter and organic matter contribute to the prevention of chronic diseases of our plant, as well as an extraction of saponins such as cardiovascular diseases and cancer due and an in vitro fermentation study. The results to oxidative stress. The ingestion of natural show that our plant Astragalus gombiformis has peptides derived from food can delay the onset of 48.48% of the dry matter, and 22.25% of the diseases by reducing oxidative damages. mineral elements, as well as 76.48% of organic matter. In addition, extraction of saponins Keywords: Antioxidant Activity; Casein indicates a concentration of 0.8ml / ml. Indeed, Hydrolysates; Lactobacillus; Protease the results of counts of protozoa reveal a reduction in the number of protozoa at different incubation times and different doses used. The forage studied has a very positive effect on protozoan reduction. This suggests another mechanism of inhibition of methane production.

Keywords: Astragalus gombiformis; Chemical analysis; Ruminants.

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P190 P191

Evaluation of the antioxidant activity of the Evaluation of the antioxidant activity of the aqueous extract of Cucumis melo seeds aqueous extract of Citrullus lanatus seeds

Amira imane bahmed1*, Amira imane bahmed1*, HaniarimBahmed2, HaniarimBahmed2,Mohamed Bouzouina3. Mohamed Bouzouina3.

Laboratory of Pharmacognosy and HerbalMedicine, Laboratory of Pharmacognosy and HerbalMedicine, University of Mostaganem 1. University of Mostaganem 1. University of Sidi Belabes 2. University of Sidi Belabes 2. Plant Protection Laboratory, University of Mostaganem 3. Plant Protection Laboratory, University of Mostaganem [email protected] 3. Abstract *[email protected]

The seeds of melon (Cucumis melo) are used in traditional medicine to cure various diseases. Abstract Indeed, they are used against dysuria, and Watermelon (Citrullus lanatus) is a plant used in possess antitussive properties, vermifuges and herbal medicine for its many healing properties. febrifuges, etc ... The objective of this study is to The objective of this study is to quantify the quantify the phenolic compounds and to evaluate phenolic compounds and determine the the antioxidant activity of the aqueous extract. antioxidant activity in the aqueous extract of the Phytochemical analyzes were performed on the seeds of this species. The seeds of the plant have seeds of the plant, in order to determine their undergone phytochemical assays, in order to levels of phenolic compounds (total polyphenols, determine their levels of phenolic compounds flavonoids, flavonols and condensed tannins), (total polyphenols, flavonoids, flavonols and and to evaluate the antioxidant activity using the condensed tannins), and to evaluate the DPPH method. The composition of the antioxidant activity using two methods: DPPH aqueous extract of C.melo polyphenols found and ABTS. The results indicated the presence of was as follows: total polyphenols 21,123 ± phenolic compounds in different proportions 0.408 mg / g.lyo, flavonols 216.623 ± 1.848 (total polyphenols 7.37 ± 0.711 mg / g.lyo, mg / g.lyo, condensed tannins 65.955 ± flavonoids 3.555 ± 0.1 mg / g.lyo, flavonols 9.916mg / g.lyo, flavonoids 1,995 ± 0,116mg 110.419 ± 3.967 mg / g.lyo, condensed tannins / g.lyo). The inhibition rate of the extract was 70 , 5 ± 13.18 mg / g.lyo), which would explain 4.885 ± 1.561 (at 20 mg / ml). The presence the existence of an anti-radical activity detected of these compounds would explain the by the method of DPPH 12,907 ± 3,437 (at 20mg existence of an antiradical activity detected / ml) and ABTS (75,315 ± 0,946 (at 20mg / ml) ml). Our results justify the use of this plant in by the DPPH method. The therapeutic activity herbal medicine and is a potential exploitable in of this plant is due to the presence of secondary various fields: agri-food (to replace synthetic metabolites. Indeed, the antioxidant property antioxidants), cosmetics, and pharmaceuticals, gives it a major interest that can be used in for the development of an alternative to synthetic various parts of the fight against cancer, and can drugs. become tomorrow a natural drug replacing synthetic drugs. Keywords: Citrullus lanatus; polyphenols; antioxidant activity; phytochemistry. Keywords: Cucumis melo;Phenolic compounds; Antioxidant activity;phytochemistry.

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P192 every one-year of breast-feeding. Breast cancer remains a serious women health problem, which Biochemical profile and risk factor’s requires early diagnosis and new treatment and assessment among women with breast cancer prevention strategies to reduce its occurrence in in Sétif, Algeria: case-control study our country. This can be achieved by increasing woman’s awareness for this disease, especially Romyla Bourouba 1*, Nadjet Diaifa 1, Salma Gharbi 1 those at high risk, as well as the evaluation of lipid parameters which may be helpful to early Department of Biology and Animal physiology, Faculty of detection of the possible neoplastic Nature and Life Sciences, University Ferhat Abbas Sétif 1, Algeria. transformation. [email protected] Keywords: Biochemical parameters; Breast cancer; Risk factors; Sétif. Abstract Breast cancer (BC) is a malignant disease which P193 represents the most common type of cancers among women. It has increased universally and Impact of plant protection products on some is considered as the second chief mortality cause microorganisms in soil cultivated by potatoe in women. This study aims to evaluate some (Solanum tuberosum L. var Sylvana) epidemiological and biochemical parameters among 60 women with breast cancer aged Aicha Hennia1*,Ouafaa Amiri1, Nardjess Brakni1, Said 1 between 35 and 70 years compared to 30 age- Nemmiche matched healthy women in Sétif, Algeria. This Departement of Agronomy, Faculty of Nature and Life study was conducted at the anti-cancer center of Sciences, University of Mostaganem, BP 188/227 Mostaganem Sétif. The epidemiological characteristics were 2700, Algeria collected by self-administered questionnaire. [email protected] Biomarkers of venous blood were analyzed by standard methods. Statistical comparison was made between study groups with Student’s t test, Abstract the significance was p < 0.05. Positive breast Phytosanitary products are used in agriculture to cancer family history and diet behavior were protect the crop from harmful organisms but can found to be significantly associated with the have undesirable side effects on the environment occurrence of breast cancer. However, education including humans. The work undertaken focused level and oral contraceptive, were not related to on soil aspect, precisely the impact of plant the development of BC. The results showed an protection products applied at two different average age of women with breast cancer (31,00 doses on the growth of soil microflora (bacteria ± 1,24) compared to controls (30,00 ± 1,11 and fungi) cultivated by Solanum tuberosum L. years). Breast cancer was significantly associated var Sylvana. the potato crop was treated with with high levels of cholesterol (1.90 ± 0.83 vs three plant protection products (Medamec, 1.52 ± 0.07) and triglycerides (1.51 ± 0.12 vs 1.10 Equation Pro and Folio Gold) at two different ± 0.03). Recent study showed a significantly agronomic doses. Collected soil samples were higher alteration of these parameters in cancer analyzed for their physicochemical and patients compared with the healthy control microbiological characteristics. The results groups and in growing aged patients and more obtained showed that the application of the first especially in women. No significant relation was dose has a significant effect on the decrease of established regarding hyperglycemia and obesity bacterial and fungal populations of the order of among BC group, despite that hyperinsulinemia 28% and 48%, respectively. These results are has been shown to be an independent risk factor confirmed by the values recorded with the NPP for breast cancer in nondiabetic postmenopausal technique (23%) and the microbial biomass women and may help to explain the relationship technique (47%). The application of dose 2 between obesity and breast cancer. There was increased this microbial decrease to 50% and significant decreasing risk of breast cancer with 67%, respectively. On the other hand, the solid enhancing periods of lactation. It has been medium enumeration technique revealed slightly estimated that there is a 4.3% reduction for 159 Oran 05-07 December

higher microbial densities (bacterial and fungal) aluminium toxicity (Al) on behavior, redox status than that observed for the control. Herbicides and leptin level was analysed by using the one and pesticides affect various microbial soil wayAnova test. The results revealed that AlCl3 processes and, depending on the type and dose of significantly increased the level of TBARS, NO, application, may alter biomass quantitatively and the antioxidant enzymes such as SOD, CATand qualitatively in the short and long term. These GPx, while GRwas significantly decreasedin the results affirm that plant protection products act cerebral cortex in intoxicated pup rats. Moreover, on soil microflora because its increase and the leptin concentration was significantly decrease reflect a disruption of its activity. increasedin cortex. On the other hand, the results exhibited that, omega-6/omega-3 dose were able Keywords: Plant protection products; microbial to ameliorate the mentioned parameters. The biomass; soil microflora ; bacteria ; fungi. Results reported that AlCl3is capable to cause marked alterations in some behavior and biochemical parameters by inducing an oxidative P194 damage and inhibiting the antioxidant enzymes activities. Whereas the administration of omega- Aluminium-induced acute neurotoxicity in 6/omega-3 combined to aluminium minimize its rats: Treatment with omega-6/omega-3 ratio hazards. In addition, omega-6/omega-3 proved to be beneficial in decreasing free radicals and Imène BENYETTOU1,2*,Nouria HALLAL2,3, Soumicha lipids level and increasing antioxidant enzymes MAHDJOUR4,Omar KHAROUBI2. activities. As well as omega-3 and omega-6 consumption could attenuate aluminium toxicity. Higher School of Biological Sciences of Oran. 31003. Oran, Algeria. Experimental Biotoxicology Laboratory Bio depollution and Keywords: Aluminum, neurobehavioral Phytoremediation. University of Oran1 Ahmed Benbella.Oran, disorders, pro/antioxidant balance, omega- Algeria. 6/omega-3 ratio. University Center of Tissemsilt.Tissemssilt, Algeria. Laboratory Productions, Plant and Microbial Valuations (LP2VM), University of Sciences and Technology of Oran Mohamed Boudiaf. Oran, Algeria. [email protected] P195 Essential trace elements and medicinal tree: Abstract When is it safe to use?

We are currently living in the "aluminum age" Bacha Mohamed El Amine 1*, Nedjimi Bouzid 1. and its exposure is inevitable. Today, its toxicity is well established in animals, and an 1 Laboratory of Exploration and Valorization of Steppe epidemiological link has indeed been Ecosystem, Faculty of Science of Nature and Life, University of demonstrated between its exposure and Djelfa, Ain Chih city, P.O. Box 3117, Djelfa 17000,Algeria. [email protected] neurodegenerative diseases, following an overproduction of free radicals responsible for damage to the nervous system and other organs. Abstract The present study evaluates neurobehavioral, biochemical and hormonal disturbances at the Traditional medicinal tree such as Juniperus brain level in 4 weeks old pups born from thurifera L. have been largely used since several mothers intoxicated by aluminum and enlarged centuries for a wide range of ailments related to to investigate the possible ameliorating role of digestive, endocrine, reproductive, and omega-3 and omega-6 fatty acids with low and respiratory systems. Additionally, it is used in high ratio. Aluminium was administered decoctions to treat vomiting and diarrhea. The intraperitoneally (100 mg kg-1 b. wt., twice a aromatic berries were chewed to counter week) to female adult rats. Postnatally (30 days), bleeding of gums. The essential oil of this species pups were given orally by gavage the low and has antioxidant, antibacterial and antifungal high ratio of omega-6/omega-3 for 5 weeks. The properties. The present study focuses on the antioxidative and protective properties of analysis of leaves of Juniperus thurifera L., for omega-6/omega-3 ratio against the effects of evaluation of Zinc and Copper in order to

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highlight the importance of this spices as a growth and the redox status in the brain potential source of micronutrients and to test the after exposure to sub-chronic (15 days) oral hypothesis when the practitioners of traditional two pesticides, a pyrethroid; deltamethrin medicine use this plant during seasons and to and an organophosphorus; phosalone, on an determine the better time during the year to animal model; rabbits of New Zealand breed, harvest plant parts when concentrations of these and on the other hand the preventive effect elements were in optimal levels using a sensitive nuclear analytical technique based on energy of a polyphenol; quercetin against this dispersive X-ray fluorescence (EDXRF). The toxicity. The experiment was carried out on accuracy of the method was evaluated by forty (40) rabbits weighing between 1.7-2.7 analyzing the Certified Reference Materials kg, were divided into eight groups of 5 (CRMs). The leaves of J. thurifera L. contain a animals each; the first group served as a higher amount of Cu (969.2 µg/g) during control, they received oral mineral water, the autumn. Zinc contents fluctuate over time, with a other groups were exposed to pesticides peak recorded during the summer (28.61 μg / g). (phosalone and deltamethrin) and quercetin, The contents of Zn and Cu in J. thurifera L. during either alone or as a mixture. Body weight the four seasons was determined and compared was determined everyday, after sacrifice by with the recommended values (RDA) and was decapitation and extraction of the brain, the found to be well below the permissible limits recommended by the Joint WHO/FAO guidelines. latter was weighed and reported in The data obtained in the present work will be percentage of the total body weight. Brain’s helpful in the synthesis of new synthetic drugs protein content was assessed according to which can be used for medicinal purpose. Bradford (1976). Glutathione peroxidase (GPx) (E.C.1.11.1.9) activity was measured using the Keywords: Juniperus thurifera L, Essential trace method of Flohe and Gunzler (1984). Glutathione elements, Medicinal tree, Dietary intake. (GSH) concentration was measured utilizing the method described by Weckberker and Cory (1988) [3]. Malondialdehyde content was measured in brain tissues according to the P196 method of Esterbauer et al. (1992). Our results Neurotoxic effect of a mixture of two show a neurotoxicity by the two pesticides pesticides in rabbits and the protective role of used (PHO and DM), following the analysis of a flavonoid: Quercetin these results, one observes a considerable reduction of body weight of the rabbits Fouad Menaceur 1*, Salim Gasmi1, Rachid Rouabhi 1, exposed to the pesticides and an increase in Brahim Benaicha 1. the relative weight of the brains. The results also showed notable changes in biochemical Faculy of Exact Sciences and Nature and Life Sciences, Larbi Tebessi University, Tebessa, Algeria 1. and enzymatic parameters reflected by a [email protected] decrease in cytosolic protein content, activity of glutathione peroxidase (GPx) and GSH. While MDA levels have increased. Our results Abstract demonstrated that quercetin can correct The intensive use of plant protection various disturbed parameters and can products, in particular insecticides, causes reduce the toxicity induced by deltamethrin adverse and phosalone either individually or as a effects on living organisms and their mixture. environment. Food is an important source of Keywords: Neurotoxicity; Oxidative stress; consumer exposure to pesticides that are Pesticides; Rabbits. associated with the appearance of various pathologies. The objective of this work was to evaluate the different neurotoxic effects such as the global parameters of animal

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P197 microorganisms directly responsible in the production of ethanol post-mortem in the Contribution to the study of post mortem presence of glucose, lactate, glycerol or amino blood alcohol concentration from peripheral acids from the degradation of proteins. These and central blood in putrefied and non- bacteria present in the digestive tract at the time putrefied cadavers of death invade the portal venous system and the intestinal lymphatic vessels in the first hours Fatma Belhoucine 1, Halim Bourorga 1,2, Mohamed after death, then the ethanol will be released to Guenaoui 1,2⋆, Amel Berrebbah-Alioua 1 finally reach the heart chambers, resulting in a significantly higher blood alcohol concentration. University of Sciences and Technology Oran-Mohamed Boudiaf, El Mnaouar, BP 1505, Bir El Djir 31000, Oran, is different than peripheral blood. The average Algeria.. heart blood alcohol levels for non-putrefied Regional Laboratory of Scientific Police of Oran. Algeria. cadavers and the blood alcohol level in the [email protected] peripheral blood.It is not significant enough, this is mainly reflected by the absence of the phenomenon of cadaver rot. The results obtained Abstract allow us to make a preliminary observation and Ethanol known as alcohol is one of the so-called to say that the elevation of alcohol levels is legal drugs in the same way as tobacco when proportional to the degree of putrefaction, more consumption becomes excessive and chronic than the state of the corpse is in advanced (ethylism), it can cause significant risks to health. putrefaction more than the alcohol level is Our current study consists of a cadaveric ethanol important. Our study shows us that in case of assay in blood from different anatomical sites, request for toxicological analysis by the medical namely peripheral blood and central blood, in examiner, it is therefore essential to correctly order to highlight the actual level of ethanol mention on the samples whether it is cardiac ingested and if it occurs. alcohol and to highlight blood or peripheral blood. It is currently the importance of the anatomical site to ensure a accepted that the post-mortem blood blood sample to have a real post mortem blood concentration of a xenobiotic is not always a alcohol concentration that reflects the state of reflection of its ante-mortem blood the person before his death. We targeted the concentration. These phenomena, grouped cardiac and peripheral blood of two populations: together under the term post-mortem rotten corpses and non-putrefied corpses. Assays redistribution, can make the interpretation of the are performed using the gas chromatograph, results very difficult, especially since they very coupled to a flame ionization detector (GC/FID). frequently concern molecules commonly This study is carried out in the context of encountered in forensic toxicology, such as criminalistic cases, received by the Department ethanol. This experience allows the toxicologist of Legal Toxicology of the Regional Laboratory of analyst, forensic scientist and investigative Scientific Police of Oran. In Algeria, the tolerable department to have an objective and constructive concentrations are <0,2g/l. In the case of dialogue on the reality of an alcoholic overtaking the measures of criminal sanctions intoxication among cadavers. will be put in place (almond/imprisonment). These checks are mandatory in all cases of crime Keywords: Post-mortem; Redistribution of or accidents followed by death. for putrefied alcohol; Cardiac and peripheral blood; cadaver results: the average blood alcohol level Putrefaction. in the cardiac blood is significantly higher than the average blood alcohol level in the peripheral blood. This raises as many questions in forensic science interpretation, especially in cases where peripheral blood alcohol has revealed a negative rate and blood alcohol concentration in the cardiac blood is positive for some cases in the first population. This positive alcoholic impregnation is relatively induced by the putrefaction phenomenon caused essentially by

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P198 resulting from the pharmacophore model, which gives a reliability of the QSAR model. Molecular QSAR Study and Molecular Modeling of POLO docking analysis of the ligand-receptor complex Like Kinase 1 inhibitors using a theoretical DFT OPLS3 basis has shown a correlation with the QSAR model and the Widad Sobhi 1,3, *,Ayoub Attoui2,Yacine Benguerba 2,3. pharmacophore model. Comparative analysis of the models obtained yielded statistically 1Laboratory of Applied Biochemistry, Université Ferhat Abbas Sétif-1, 19000 Setif Algeria significant results. These models showed a good 2Laboratoire des Matériaux Polymères Multiphasiques, response to the validation of the set of tests. All LMPMP, Université Ferhat Abbas Sétif-1, 19000 Sétif, Algeria structural informations obtained from the QSAR, 3National Research Center in Biotechnology , CRBt , 25000 the electronic descriptors calculated by DFT Constantine, Algeria . [email protected] BP86, COSMO, and OPLS3 are compatible with the available crystal structure of PLK1 in combination with BI2536 where the Abstract pharmacophore model helps to better interpret Polo-like kinase 1, an important enzyme with the structure-activity relationship. various biological actions in cell mitosis, is a Key words: PLK1, docking, QSAR, DFT, promising target for the development of new pharmacophore anticancer drugs. The present work is part of the study of the antitumor activity PLK1 kinase inhibitors using the modeling method QSAR, modeling the pharmacophore and molecular P199 docking approach using the methods molecular Inhibition of Candida albicans biofilm by modeling. The objective is to develop stable QSAR models, reliable and predictive for the synergistic action of exogenous terpenoids prediction of inhibitory power to target PLK1. A Hidaya Fatima Zohra TOUIL1*, Kebir Boucherit 1,2, molecular docking approach, structure-based Zahia Boucherit-Otmani 1,2. pharmacophore modeling, and a quantitative structure-activity relationship (QSAR) study 1Laboratory: Antifungal Antibiotic, Physico-Chemical were performed on a set of molecules as PLK1 Synthesis and Biological Activity, University of Tlemcen, inhibitors. The common substructure, molecular Algeria. 22, Rue Abi Ayed Abdelkrim Fg Pasteur B.P 119, 13000, Tlemcen, Algeria docking and pharmacophore alignment were Aboubekr Belkaid University, Tlemcen 13000. used to develop a 3D-QSAR model. Several QSAR [email protected]; [email protected]; models were obtained using electronic [email protected] descriptors calculated by the method (DFT / BP86 / def-TZVP basis set (triple-valence Abstract polarized) and descriptors that reflect the hydrophobicity /hydrophilicity of the studied Candida albicans is opportunistic yeast, molecules, descriptors based on the so-called responsible for systemic infections in individuals group contribution method and physico-chemical with impaired immune response. Nosocomial C. descriptors. The best model was obtained using albicans infections are often related to the ability the MLR method analysis. It shows that the to produce biofilm on mucosal surfaces and combination of the HOMO descriptor and the implanted medical devices. Candida albicans number of H-bond donors (HBD) and H-bond Biofilms contribute to increasing unpleasant acceptors (HBA), can explain 92% of the mortality rates which pose an important variation of antitumor activity, quantified by therapeutic challenge in human diseases. Usually, pIC50 of 41 studied compounds. The retained conventional antibiotics agents encounter QSAR model reveals that the low HOMO values difficulty in treating and fully eradicating biofilm- combined with HBD and HBA lead to an increase related infections. So, several therapeutic in the efficacy of the PLK1ce inhibitors, which approaches are needed to treat recalcitrant implies that the electronic effects play a very Candida albicans biofilms. The use of natural important role in explaining the inhibition compound as an alternative source of mechanism. The calculated surfaces of the sigma antimicrobials has become a necessity given the profile are compatible with the HBAs and HBDs growing concern over global antimicrobial

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resistance. Our study aimed to investigate the Nicotinamide/Streptozotocin (N/STZ)-induced possibility of using natural compound within the diabetic rats. Extraction of Nigella sativa oil was context of healthcare as a way of inhibiting and carried using methanol/chloroform extraction. preventing the harmful development of Candida Neutral lipids were obtained by fractionation on albicans biofilm. The study of the susceptibility of silica gel column. Both total oil and neutral lipid Candida albicans to terpenes was determined fractions were assessed by gas chromatography using the microdilution method according to (GC) for their contents on fatty acids, CLSI. A Checkerboard assay was employed to phytosterols and phytostanols. Triglycerides evaluate the efficacy of terpenoids combinations. contents were determined using high Biofilm susceptibility was determined using a performance liquid chromatography (HPLC) metabolic 3-(4,5-dimethylthiazol-2-yl)-2,5- coupled to an evaporative light scattering diphenyl tetrazolium bromide (MTT) [3]. Out of detector (ELSD). After N/STZ induction of the two terpenoids, carvacrol showed a stronger diabetes in Wistar rats, fractions were anti-Candida activity with MIC ~1 mg/mL. Both administered orally (100mg/kg/daily) for 21 carvacrol and cuminaldehyde were able to days, and blood glucose and lipids levels were reduce the viability of pre-formed biofilm at assessed. Neutral fraction is rich in various different concentrations. Finally, the combination bioactive lipids. In diabetic rats group treated of these terpenoids (carvacrol/cuminaldehyde) with either total oil extract or neutral fraction, revealed an interesting synergistic effect and blood glucose decreased significantly from 123 may prove more useful as chemotherapeutic mg/dL to 83 mg/dL respectively after only 7 agents than such compounds used singly. The days. Afterwards, blood glucose was stabilised to results from this study suggested that the normal levels in both treated animal group from combination carvacrol/ cuminaldehyde seems to 15th to 21st day of experiment. A significant be a new strategy against C. albicans biofilm- decrease in triglyceride was observed within related infections, and can be hence used as only one week of treatment with both fractions. preservative to surgical devices without In contrast, treatment with both total oil and developing toxic effects on human neutral lipids fractions lead to an increase in high density lipoprotein (HDL) cholesterol levels to Keywords: Candida albicans, terpenoids, 1.19 ± 0.08 g/L and 0.78 ± 0.08g/L respectively antibiofilm activity. at the 7th day with no change in body weight of animals during experiment. Neutral lipids play an important role in stabilising blood lipids and P200 reduce significantly hyperglycaemia in diabetic rats. This finding would suggest them for the Neutral lipids for treatment of treatment of diabetes and dyslipidemia. hyperglycaemia and dyslipidemia: First results on an animal model Keywords: Nigella sativa, Hyperglycemia, Dyslipidemia, Neutral lipids. Widad Sobhi 1,3, *,Yacine Benguerba 2,3, Mounira Amrane1, Abdelhalim Khenchouche1 P201

Acute oral toxicity of the aqueous extract of 1Laboratory of Applied Biochemistry, Université Ferhat Abbas bryonia dioica roots Sétif-1, 19000 Setif Algeria 2Laboratoire des Matériaux Polymères Multiphasiques, LMPMP, Université Ferhat Abbas Sétif-1, 19000 Sétif, Algeria Nour EL-HoudaFeriel Djebara*; Bachir Benarba 3National Research Center in Biotechnology , CRBt , 25000 Constantine, Algeria . 1Universiy of Mustapha StambouliMascara, Biology [email protected] ; [email protected] department Code postal: Mascara2900 nourferial @yahoo.fr Abstract This study is an investigation into the effect of Abstract neutral lipids of Nigella sativa seed on Bryonia dioica Jacq is a climbing perennial herb hyperglycemia and blood lipid status in with its roots which is generally used in tradition 164 Oran 05-07 December

al medicine in Algeria for the treatment of breast the majority of solid tumors, the early growth cancer.Our study aimed to investigate the safety phase of a primary tumor and its metastatic of the aqueous extract of Bryoniadioica roots dissemination process are largely dependent on growing in Algeria by acute toxicity study. The in the formation of new vessels (angiogenesis vivo acute oral toxicity studyof aqueous extract tumor), without which the tumor cells remain as of Bryonia dioica was conducted in accordance dormant cells, stable state intermediate between with Organisation for Economic Co-operation proliferation and apoptosis. Among the and Development (OECD) 420 Guideline. The identified angiogenic factors, vascular aqueous extract of Bryonia dioica root was endothelial growth factor (VEGF) is the key administrated in a single dose of 2000 mg/kg via regulator of tumor angiogenesis. It is a oral gavage; and the animals were observed for glycoprotein expressed deregulated by tumor any behavioral changes or mortalityfor 14 days cells. VEGF-targeting therapies would normalize then the rats were sacrificed for hematological, tumor vascularization and thus facilitate access biochemical and histopathology studies, body of chemotherapeutic agents to tumors. We weight, organ weight were also evaluated. Acute propose in this work to inhibit VEGF and to study administration of 2000 mg/ kg of the aqueous the interaction between VEGF and organic extractof Bryonia dioica root did not induce any molecules, based on data from the literature mortality and no toxicological symptoms were (Axitinib, Sorafenib, Sunitinib and NSC 35676). observed within 14 days. In addition, there were Based on the total interaction energy, it was no significant elevations observed in the noted that among all inhibitors, Sunitinib 3 is the hematological parameter such as RBC WBC best inhibitor of all series studied. Calculation of platelets and no significant changes in. ALT, AST, the distances between the Sorafenib1 and the urea and creatinine level. Moreover, no adverse amino acid side chains constituting the active site changes in themacroscopical aspect of organs and the calculated energies confirm that and in relative organs weight like heart, lungs, Sunitinib 3 has a better complementarity with liver, spleen and kidneyin the animals. Our the enzyme VEGFA. We can conclude that results showed that acute administration ofthe Sunitinib3, has a better inhibition to slow the aqueous extract of Bryonia dioica root did not evolution of the studied enzyme. According to the produce any sign of toxicity or death in rats, literature the three ligands Axitinib, Sorafenib suggesting that the LD50 ishigher than and Sunitinib, are inhibitors of VEGFR. According 2000mg/kg. to our theoretical study these three ligands inhibit VEGFR and VEGFA. In perspective the Keywords: Bryonia dioica root, acute oral next steps will consist of using other molecular toxicity, biochemical parameters and docking programs, among the most recent and hematological parameters, aqueous extract. the most efficient that we have used, to test all the inhibitors of different enzymes studied so far in order to propose the best ones. We must also take into account the solvent effect that has been P202 neglected in our approach. Inhibition of VEGF during angiogenesis Keywords: VEGFR; VEGFA; molecular docking; Axitinib, Sorafenib; sunitinib. Nawel Amraoui1*,Majda Dali-sahi1,Takwa Salmi1, Meriem Benyarou2,Said Ghalem2.

1 Analytical Chemistry and Electrochemistry Laboratory Biology and Health Team, Université de Tlemcen, ALGÉRIE. 2 Research Laboratory Natural and Bioactive Substances "LASNABIO". *[email protected]

Abstract Angiogenesis is fundamental and essential for tumor development and metastatic spread for

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P203 proteomic is considered as rapid instrument for detection the emergence of a XDR strains Molecular characterization of multidrug harboring a new genotype with a high risk in resistant Proteus mirabilis isolates from ambulatory and hospital care. Algerian patients Keywords: Proteus mirabilis; AmpC; Extended- Hadjer Boudjemaa*, Rachida Allem spectrum β-lactamase; ESBL; Aminoglycoside- Laboratory of Natural Bio resources, Department of Biology, modifying enzyme; Algeria. Faculty of Science, University of Hassiba Ben Bouali Chlef, Box 151, 02000 Chlef, Algeria. * [email protected] P204

Abstract Complete chloroplast genome of the diatom Skeletonema pseudocostatum from the The aim of this study was to characterise the western mediterranean coast of Algeria molecular genotype of multidrug resistance in

Proteus mirabilis isolated from Algerian patients. Chahinez Hamedi1, Romain Gastineau2, Claude A total of 217 P. mirabilis isolates were collected Lemieux3, Monique Turmel3, Andrzej Witkowski2, from two hospitals and eight private laboratories Mohammed Bey Baba Hamed4 from four cities located in northwestern Algeria. 1 All isolates were identified by Ap20E system and Laboratoire d'Aquaculture et Bioremédiation (AquaBior) Université Oran 1 Ahmed Ben confirmed matrix-assisted laser Bella, BP 1524, El Menaouer, 31003 Es Senia, Oran, Algeria. desorption/ionisation time-of-flight mass 2Institute of marine and environmental sciences, University of spectrometry (MALDI-TOF/MS) with score up to. Szczecin, Al. Papieża Jana Pawła II 22a 70-453 Szczecin, Antimicrobial susceptibility testing was Poland. 3Département de biochimie, de microbiologie et de bio- performed by the disk diffusion. Genes encoding informatique, Institut de Biologie Intégrative et des Systèmes, AmpC β-lactamases, extended-spectrum β- Université Laval, 2325 Rue de l'Université, Québec, QC G1V lactamases (ESBLs), quinolone resistance and 0A6, Canada. aminoglycoside-modifying enzymes (AMEs). 4Ecole Supérieure en Sciences Biologiques d’Oran (ESSBO), BP Clonal relationships were also determined by 1042 Saim Mohamed (EX-IAP) 31003 Oran, Algeria. *[email protected] MALDI-TOF/MS proteomic typing. Of the 217 samples, 6.5% are highly resistant to majority of Abstract antibiotics (BHRe), of which 4/14 (1.8%) had an Skeletonema is a genus of centric, planktonic ESBL genotype (blaCTX-M-2) and (4.6%) had an diatoms that belongs to the Thalassiosirales. AmpC/ESBL genotype (blaCMY-2/blaTEM-1). Members of this genus are distributed worldwide AME genes were detected in all isolates, and can produce massive blooms. Some species including ant(2′′)-I, aac(3)-I, aac(6′)-Ib-cr and are commonly used in aquaculture for feeding aac(3)-IV. The qnrA gene was identified in bivalves and crustaceans and can also serve as majority of isolates (6%). This multiple models in ecotoxicological. Despite the numerous phentypes indicated clearly that are new studies devoted to Skeletonema, no chloroplast emergence of clonal population in hospital and genome sequence is currently available for any ambulatory care unit, this can attributed to species. In the framework of a collaborative limited effective antibiotic choose.MALDI- public-private partnership between the TOF/MS proteomic showed a one clade, include University of Oran 1 (Algeria), the ESSBO all Extremely Drug Resistant (XDR) P.mirabilis, (Algeria) and the WiraGen company (Algeria) the pseudogel indicated that there are a aiming at developing bioprospecting, molecular proteomic similarity between this strains. These taxonomy and blue biotechnologies in North results reflect a high degree of discrimination Africa, we have undertaken several sampling power for the rapid detection of clones with a campaigns of the coastal waters of Algeria. high epidemiological risk. Here we report the During one of our sampling campaigns, a strain description of plasmid-mediated quinolone of Skeletonema pseudocostatum Medlin was resistance genes and ESBL- and/or AmpC β- sampled near Cap Falcon and was isolated under lactamase-producing P. mirabilis isolates from inverted microscope. A total cellular DNA was Algerian healthcare centres. The MALDI-TOF/MS extracted from a scaled-up culture and used for

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sequencing the plastid genome. The Skeletonema pool. We observed that ionomycin-induced pseudocostatum genome is 127,013 bp long and increases in [Ca(2+)]i in T-cells of diabetic contains an inverted repeat region of 18, 240 bp. mothers and obese offspring were greater than The order of genes on the inverted repeat was in those of control rats. Furthermore, feeding found to be highly conserved when compared PUFA/EPAX diet diminished significantly the with the plastid genomes of other ionomycin-evoked rise in [Ca(2+)]i in diabetic Thalassiosirales. As expected, the maximum and obese animals. TG-induced increases in likelihood phylogeny we inferred from multiple [Ca(2+)]i in T-cells of diabetic pregnant rats and plastid genes placed Skeletonema their obese offspring were greater than in those pseudocostatum within the Thalassiosirales. This of control rats. The feeding of the experimental diatom plastid genome is the first made available diet significantly curtailed the TG-evoked for the cosmopolitan and ecologically important increases in [Ca(2+)]i in both diabetic and obese genus Skeletonema. Similar genomic studies on rats. Together, these observations provide other diatoms from Algeria are underway. evidence that T-lymphocyte activation and T- lymphocyte calcium signaling are altered during Keywords: aquaculture; chloroplast genome; gestational diabetes and macrosomia. Hence, phylogeny; Skeletonema. dietary fish oils, particularly eicosapentaenoic acid and docosahexaenoic acid, may restore these T-lymphocyte abnormalities. 205 Keywords: Diabetes, rats, T-lymphocytes, PUFA, T-lymphocyte calcium signaling modulation intecellular free Calcium. by n-3 fatty acids in obese macrosomic rats

Guermouche B, Soulimane N, Merzouk H, Hichami A, P206 Khan N. Genotyping by PCR-RFLP analysis of the 16S Laboratory of Physiology, Physiopathology And Biochemistry Of Nutrition, Faculty Of Sciences Of Nature And Life And gene fragment of hydrocarbon-degrading Science Of Eart And Univer University Aboubekrbelkaïd, bacteria isolated from contaminated marine Tlemcen 13000, Algeria sediments at the port of Oran (Algeria)

Faiza Bendadeche1*, Mohamed Bey Baba Hamed2, Abstract Sidi-Mohammed El-Amine Abi Ayad1 We investigated the effects of a diet containing EPAX-7010, rich in polyunsaturated fatty acid 1Laboratory of Aquaculture and Bioremediation (AquaBior), Department of Biotechnology, Faculty of Nature and Life (PUFAs) such as eicosapentaenoic acid [20:5(n- Sciences, University of Oran 1 Ahmed Ben Bella, Oran, Algeria. 3)] and docosahexaenoic acid [22:6(n-3)], i.e., a 2Higher School of Biological Sciences of Oran, Algeria. PUFA/EPAX regimen, on T-lymphocyte activation *[email protected] in diabetic pregnant rats and their obese pups. Mild hyperglycemia in pregnant rats was induced Abstract by intraperitoneal injection of streptozotocin on Identification and molecular typing of bacterial Day 5 of gestation. T-cell blastogenesis was strains is necessary for consideration of assayed by using (3)H-thymidine, whereas outbreaks, epidemiological studies, and intracellular free calcium concentrations differentiation of human reinfection (Benkirane, ([Ca(2+)]i) were measured by using Fura-2 in 2006), and various environmental studies diabetic pregnant rats and their obese offspring. concerning bioremediation. Detection of Concavalin-A-stimulated T-cell proliferation was polymorphisms by PCR-RFLP has several decreased in both pregnant diabetic rats and advantages including the rapid and easy their obese pups as compared with control application, explanation and use for large animals. Feeding the PUFA/EPAX diet restored T- numbers of samples. Genotypic characterization, cell proliferation in both groups of animals. We based on the analysis of restriction fragment also employed ionomycin, which at 50 nM opens length polymorphism of the 16S gene fragment calcium channels, and thapsigargin (TG), which PCR product (16S PCR-RFLP), was performed on recruits [Ca(2+)]i from endoplasmic reticulum members of hydrocarbonoclastics bacteria. The

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PCR universal primers were allowed for the from a step to another until their 3d structures amplification of approximately 1500 bp DNA are solved and/or work on them has stopped. fragment from each of the 11 bacteria species. STAD provides a number of methods for Amplified 16 S fragments were compared using information retrieval; the ‘Direct’ search method RFLP analysis after digestion using two can be used to find out detailed information endonucleases simultaneously (Nco1, Rsa1), thus about the sequence of the targets, the stages of allowing the detection of characteristic patterns their production and structure determination. of RFLP products for most interesting bacteria ‘Patterns/motifs’ search allows for finding species. Five specific RFLP groups were targets with certain sequence motifs. Targets identified among most of the bacterial species with overall sequence similarity to other public tested. The variability of genetic patterns within sequences are found by using the ‘Sequence these bacteria could be explained in terms of Alignment’ search method. In addition to basic their different genus appurtenance. The results annotation of the targets, automatic basic indicated that PCR-RFLP analysis of the 16S gene structural and functional prediction is carried out fragment is a useful molecular tool for and annotated, for the targets that structure has identification of species, as well as for not been solved yet. Such analyses are also differentiation of bacteria strains. provided in the results page. More tools are provided for further exploration of the targets Keywords: DNA 16S; fingerprinting; including a Status page, Statsboard (for overall hydrocarbonoclastic bacteria; PCR-RFLP simple statistics, see main web-site) and a Structural Gallery; http://www.bioinformaticstools.org/stad/galler P207 y.php?Lab_=All . At Saida University, a number of nine (09) targets has been selected for 3D- Structural Targets Annotation Database – structural and functional studies including STAD & PR-Proteins selected targets Dihydrofolate Reductase, Insulin and seven Pathogen-Resistant proteins (PR-proteins) a number of are private to be released at later Abdellahi Ould Ahmed Salem1 & Abdelkrim stage; can be seen at the link: Rachedi1,2,* http://www.bioinformaticstools.org/stad/srch_t rgs.php?lab_=sbb&stp=200 Unless targets are 1Department of Biology, Faculty of Sciences, Saida University, BP 138 cité ENNASR 20000, Saida, Algérie. tagged as private based on source lab 2Previous related address: Wits-Bioinformatics Unit, School of instructions, targets’ details from all contributing Molecular and Cell Biology, University of the Witwatersrand, institutes and international public targets Private Bag 3, Wits 2050, South Africa. together with the Document Type Definition * [email protected] (DTD) are made available for download in xml

Abstract format; http://www.bioinformaticstools.org/stad/galler Available online at the link: y.php?ns=2. http://www.bioinformaticstools.org/stad/ (main web-site), STAD is a database that annotates and Keywords: Annotation, Structural Targets, X-ray stores information pertaining to structural crystallography, NMR, Cryo-EM, Domains targets (mainly proteins selected for 3d- prediction, Function prediction, Cancer, HIV. structure and function elucidation) understudy at laboratories in a number of Algerian, Moroccan and South African institutes; refer to Targets Contributing Member Institutes page: http://www.bioinformaticstools.org/stad/STsou rces.php . Targets in the STAD database have been selected, by the relevant laboratories, serving medical and biotechnology objectives including cancer, HIV, diabetes and may other research axes. The database is used for tracking the progress of the targets as they are moved

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P208 correlated. Regarding relative growth displayed negative allometries, reflecting a slower growth Relative Growth of Sepia officinalis Linnaeus, rate in total weight than in mantle length during 1758 (Cephalopoda: Sepiida) from the Oran the species ontogeny. bay, Algeria Keywords: Sepia officinalis, Weight-length Leila Saddikioui1,2* & Sidi-Mohammed El-Amine Abi- relationships, Relative growth, negative Ayad1 allometries.

1Laboratory of Aquaculture and Bioremediation (AquaBior), Department of Biotechnology, University of Oran 1, Oran 31000, Algeria. P209 2Ecole Supérieure en Sciences Biologiques d’Oran (ESSBO), Oran 31000- Algeria. Evaluation of the antioxidant activity by DPPH *[email protected] and FRAP of the crude hydromethanolic extract prepared by maceration of the aerial Abstract part of Salvia officinalis The importance of cuttlefish as a global resource of fishing continues to increase, but it’s very Souad Mahdi, Rachid Azzi1, Farid Lahfa. short life cycle and variable growth rates make 1Antifungal Antibiotic Laboratory: Physico-chemistry, its stock volatile and vulnerable. Several studies Synthesis and Biological Activity. Biology Department. : have been devoted to the study of the biology of Faculty of Sciences of Nature, Life and Sciences of the Earth Sepia officinalis on several Mediterranean and and the Universe. University Abou-BekrBelkaïdTlemcen Atlantic areas on different aspects. However, in (Algeria). *[email protected] Algeria, no scientific studies on growth and reproduction parameters of this species have been published. While it is necessary to dispose Abstract detailed scientific information on its biology for a The overproduction of free radicals within the better management of its stock. The present human body and the deficit of its defense system study reports the weight-length relationships can lead to various pathologies including cancer, and assesses the relative growth of S. officinalis atherosclerosis, Alzheimer's and Parkinson's from the Oran bay. S. officinalis specimens used diseases. Currently, the research aims to in this study were sampled monthly from Oran reinforce these endogenous defenses by non- bay, between January 2013 and December 2013. toxic natural substances from medicinal plants, In total, 581 specimens, 328 females and 253 which endowed with antiradical properties[1] males were sampled and treated in detail at the (Asadi et al., 2010).The interest of this work is laboratory. The length and weight of individuals the enhancement of the antioxidant activity of were recorded to the nearest 0.1 cm and 1 g the crude hydromethanolic extract prepared by respectively. The relationship of size - weight maceration of the aerial part of Salvia officinalis. was fitted to the equation: BW = a MLb, where This activity tested by two methods: the BW is the weight (g), ML the dorsal mantle length antiradical test, which consists in estimating the of the cuttlefish (mm), a constant and b the rate trapping capacity of the free radical DPPH and of allometry, parameters a and b of the length- the chelating power of iron. The free radical weight relationship was estimated by linear DPPH is a synthetic radical of violet color that regression analysis based on logarithms: log turns to yellow when it captured by the (BW) = log (a) + b log (ML). The allometric antioxidant compounds tested. To evaluate the scaling of females and males of S. officinalis is chelating power of our extract, we adopted the lower than 3 indicating a negative allometric technique described by Wang et al. (2004). The growth. Similar findings have been reported by obtained results demonstrate the effectiveness of [1] on S. officinalis of Senegal, [2] in English the sage extract in trapping the DPPH radical, Channel, [3] in Mauritania and by [4] in Eastern translated by the inhibition rate according to Libya. In males, females and sexes combined, the different concentrations. This evolution is dose- W-L relationships were highly significant, and dependent; it increases with the increase of the the morphometric parameters were highly extract concentrations with an IC50 of 528.17 ±

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3.23μg /mL. In a similar study on methanolic hand, the β-carotenebleaching test shows that extracts of various organs of the sage, the essential oils of our plant preserve the color Grzegorczyk et al. (2007)[3] also show efficiency of β-carotene in a mannersimilar to that of in trapping the DPPH (with IC50 between 18.4 catechin and gallicacid, whichreveals a and 81.7μg /mL). In addition, this extract has a considerableantioxidant power. It would be very high chelating power with an EC50 of the order interesting to complete this phytochemical study of 357.14 ± 0,02μg /mL. Salvia officinalis by isolating, purifying and identifyingother possesses important medicinal effects that will classes of secondarymetabolites, as well as have to be valued. Thus, it would be very checkingotherbiologicalactivities in order to interesting to complete this study by an in vivo establishascientific basis for the traditional uses experiment using an animal model. of the plant.

Keywords: DPPH; FRAP; Salvia officinalis. Keywords: Naupliusgraveolens, essential oil, antioxidant activity, Algeria

P210 P211 Biological valorization for essential oils of TAFFS in the south-west Algerian Cytogenetic and clinical study of Turner syndrome and klinefelter in the population of Benaissa keddar Youcef, Megherbi Aicha, Mohammed El-Amin Said¹, Benyamina abdelfetah western Algeria

University of Djilali Liabes Sidi Bel Abbes, Laboratory of Zoulikha Boucif Debab¹-²*, Tewfik. Sahraoui², EcodevelopementSpaces, BP 89, 22000 Sidi Bel Abbes, Fatima Zohra. El Kebir², Aicha. Idder ¹. Algeria. *[email protected] 1Ophthalmology E.H.S Oran. Medical Genetics Laboratory applied to ophthalmology. ALGERIA. 2University of Oran 1. Laboratory developmental biology Abstract and differentiation. . ALGERIA. The valorization of natural resources is based on *[email protected] the extraction of their bioactive substances, Abstract especially the essential oils with regard to The hétérosomiques diseases are usually caused aromatic medicinal plants. Among these plants, by aneuploidy nondisjunction, they hit a boy on we focused on the evaluation of the antioxidant 400 and 650 on a girl with some form of sex activity of a plant of the Asteraceae family chromosome abnormality [1, 2]. The aim of our growing spontaneously in Algeria, which is an study is to analyze mitosis to establish the endemic medicinal species. The aim of our work percentage of the different karyotypes, and prove is to test the efficacy of essential oils of the existence of a karyotype phenotype Naupliusgraveolens originating from correlation. This study was conducted at the southwestern Algeria against oxidative stress by Cytogenetics Laboratory of Ophthalmology EHS using two methods (DPPH free radical trapping Oran, on a sample of 2306 patients, she treated and β-carotene bleaching test). The extraction of two syndromes: essential oilsiscarried out by - The Turner syndrome, hydrodistillationwith the aid of a - Klinefelter syndrome, clevengerapparatus. Twomethods have been It was extended over a period of ten years chosen for the evaluation of the (January 2006 to December 2015) because these antioxidantactivity, thisis the free radical conditions are relatively rare among live births, scavenging test (DPPH) and the bleaching test of although they are common to the design. The β-carotene. Theresultsobtainedrevealthat the medical orientation was established by clinicians essential oils of the leaves and flowers do not practicing in the following specialties: representanyinhibitoryeffect of the DPPH radical endocrinology, gynecology and pediatrics. A in comparisonwiththree standards (gallicacid, genetic study was performed for each patient, it catechin and ascorbicacid), these latter showed a is to perform an examination to determine the considerableantioxidant power. On the other patient's age, sex, the concept of consanguinity,

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in the case of death of siblings and their causes, phenolic contents. The results of the anti- its geographical Providence …With these results , hemolytic activity show that all the extracts we demonstrated the role assigned to the possess a maximal inhibitory effect against the cytogenetic study, through chromosomal hemolysis caused by H2O2 with percentages formulas that reflect the phenotypic differences ranging from 57.48 to 60.52%. Our study shows observed in both syndromes. In Algeria, the that the fruit of Gleditsia triacanthos is an hétérosomiques diseases, exist and affect several important source of active ingredients, families [3]. The karyotype is a capricious considered as natural antioxidants and as craftsmanship subject to the vagaries of the cell protective agents against hemolysis induced by culture is a valuable tool, it allows confirmation oxidative stress, which is involved in several of the diagnosis (syndrome suspected by the diseases. clinician), the establishment of a regimen and the establishment of genetic counseling. Keywords: anti-hemolytic activity; antioxidant activity; extract; Gleditsia triacanthos. Keywords: cytogenetics, sex chromosomes, karyotype, phenotype P213

P212 Study of the antioxidant activity of extracts of Scolymus hispanicus L. leaves Antioxidant andanti-hemolytic effects ofphenolic compounds of Gleditsia Benchaachoua Abbassia1, 3*, Saidi Imène1, 3, Mahdjoub triacanthos L. Pods Bessam Hassiba1, 2.

Imène SAIDI 1, 3,*, Abbassiabenchaachoua1, 3, Hassiba 1Laboratory "Eco-development of spaces" Faculty of Mahdjoub Bessam1, 2 Sciences of Nature and Life 2 Djilali LIABESUniversity -Sidi Bel Abbès-ALGERIA 3 1Laboratory "Eco-development of spaces" Faculty of Department of Biology, Faculty of Sciences of Nature and Sciences of Nature and Life Life Mustapha StambouliUniversity Mascara-ALGERIA 2Djilali LIABÈS University -Sidi Bel Abbès-ALGERIA [email protected]; [email protected]; 3Department of Biology, Faculty of Sciences of Nature and [email protected] Life Mustapha Stambouli University -Mascara- ALGERIA Abstract *[email protected] By its geographical location, Algeria offers a vegetation rich in aromatic plants that grow Abstract spontaneously [1]. As a result, we focused on the Gleditsia triacanthos L. is a plant that belongs to valuation of the wild plant Scolymus hispanicus. the family of Fabaceaeor Legumes [1],widely L of the asteraceae family [2] for the purpose of used in traditional medicine for its therapeutic researching new bioactive products of natural properties [2].This study carried out in order to origin benefiting from biological activities evaluate the anti-hemolytic and antioxidant including antioxidant activities. The effect of Gleditsia triacanthos L pod extracts. The quantification of the phenolic compounds was pods extracted with 70% methanol, 70% ethanol estimated by the Folin-Ciocalteu method[3]and and water. Colorimetric methods using Folin- the flavonoids by the AlCl3 method[4]. The Ciocalteu reagent, aluminum chloride carried out evaluation of the antioxidant activities of the to estimate total polyphenols and flavonoids. The various extracts was determined, in vitro, antioxidant activity were determinated by in through the following tests: total antioxidant vitro methods [3,4].and the anti-hemolytic capacity and DPPH˙[5]. The results obtained on activity by induction of hemolysis by H2O2, on the tested extracts show that the extracts that the red blood cells of a healthy patient. The hydroacetonic extract gave the best polyphenols quantification of phenolic compounds shows the (52.69 ± 0.0536 mg EAG /gMS) and flavonoids richness of the methanolicextract in total (44.81 ± 0.014 mg EC / g MS) compared to the polyphenols (13,183 ± 0.873mg EAG / g MS) and other testedsolvents.The results of these assays flavonoids (6,223 ± 0,331 EQ / g MS). The show that the aqueous extract has a strong total methods of the antioxidant activity invitro show antioxidant capacity (66.67 0.015 mg EAG/g MS) that all the extracts studied have antioxidant compared to other extracts and with a strong properties at different levels depending ontheir

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DPPH radical scavenging ability compared to P215 positive control. The results obtained from the Phytochemical analysis and evaluation of the study of the antioxidant activity of the leaves of Scolymus hispanicus L in vitro are only a first antioxidant activity of the aqueous and step in the search for substances of biologically methanol extracts of Rhus pentaphylla Desf. active natural origin, an in vivo study is desirable, Benmansour Nassima1*, Allal Asma2, Selles Chaouki2 to obtain a more in-depth view on the 1Laboratoire de spectrochimie et pharmacologie structurale antioxidant activities of this plant. (LSPS), département de chimie, université de Tlemcen, Tlemcen, Algérie. Keywords: DPPH; Phenolic compounds; 2Laboratoire des substances naturelles et bioactives Scolymus hispanicus L ; Total antioxidant (LASNABIO), département de chimie, université de Tlemcen, Tlemcen, Algérie. capacity. * [email protected]

Abstract P214 Several epidemiological studies suggest that The antioxidant activity of the aerial part of plants rich in antioxidants such as flavonoids and Menthaspicatain region of the AL BAYADH, polyphenols play a protective role in health and ALGERIA against diseases, and their consumption lowered the risk of cancer, heart and kidney diseases [1]. Medjaher Halima Essaadia Souhila, Benchohra The aim of this study is to determine the content Amelhadria, Dif Mustapha Mahmoud, Ghribi Amina of polyphenols, flavonoids, and condensed tannins contents of fruits, leaves and roots the Laboratory of Ecodevelopment of Spaces, (Faculty of Rhus pentaphylla Desf. The antioxidant activity Natural Sciences and Life, University of DjilaliLiabes, Sidi Bel of methanol and aqueous extracts was then Abbes, 22000) evaluated. Phytochemical screening was carried *[email protected] out by characterization reactions based on coloring or precipitation and observations. The Abstract total phenolics (TPC), flavonoids (TFC) and Menthaspicata, is an aromatic and medicinal condensed tannins (CTC) contents were plant, the aerial part of determined by using spectrophotometric thisLamiaceaeiswidelyused by the locals people methods. The antioxidant activity of extracts was leaving in the region of AL BAYADH, for its evaluated with the use of two assays (FRAP and therapeutic vetus. The purpose of this study is to DPPH). The phytochemical tests revealed the evaluate the anti-oxidant effect of the aerial part presence of flavonoids, tannins, steroids, of the mint (leaves and stems) of an arid zone. mucilage and saponins. The highest levels of TPC The sample harvested from the AL BAYADH zone and CTC were found in roots extracts, with the is dried, then crushed and macerated with values of 44.00 ± 0.09 mg GAE/g and 39.74 ± ethanolfor 24 h, after filtration, the evaluation of 0.08 mg QE/g, respectively. Furthermore, lower the antioxidants was carried out by trapping the quantities of TPC (9.30 ± 0.09 mg GAE/g) and free radicals at the DPPH. The free radical CTC (3.86 ± 0.03 mg QE/g) were observed in scavenging by DFPPH indicated a very high anti- fruits extracts while the highest quantity of TFC oxidation power at the leaf level with an IC50 of (8.52 ± 0.03 mg CE/g) has been recorded in the 1,82 mg/Lat the 11,33mg / Lstems. These leaves extract. Otherwise, aqueous and methanol observed antioxidant activities are due to a very extract of the roots showed the best antioxidant large production of secondary metabolites. The activity (IC50 values were respectively 0.71 ± results obtained show that the methalonic 0.04, 0.11 ± 0.01 mg/mL for DPPH and 0.38 ± extracts prepared have a very interesting 0.05, 0.10 ± 0.02 mg/mL for FRAP assays). Rhus reducing power, so the aerial part of the mint pentaphylla. contains bioactive substances that (leaves, stems) from the Al BAYADH region could be used as a new promising source of represents a source of antioxidant. antioxidant compounds in different applications.

Keywords: Menthaspicata; lamiaceae; AL Keywords: antioxidant activity, Rhus pentaphylla, BAYADH, ALGERIA; Antoxidant. phytochemical screening.

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P216 genotypic combinations, a greater frequency of the AA genotype was observed in patients: Genetic susceptibility to tuberculosis: OR=2.26 [0.99-5.16]; p=0.049. On the other hand, Association of the polymorphism IL10 -1082 the GG genotype was more frequent in the G/A to active tuberculosis in a Western controls: OR =0.29 [0.13-0.62]; p=0.001. Several Algerian population studies described the functional impact of this polymorphism in the production of IL-10[3]. This Mohamed ghermi1,3*, Aïchabelgacem1, Khadidja polymorphism, associated with tuberculous Attab1, N. Ghoumari2, N. Mened2, F. Saichi2, S. Bossi2, infection in many populations, seems to be also Mohamedbaba Hamed El Bey3 within Oran’s population. The analysis of other polymorphisms located in other genes involved 1Département de Biotechnologie, Université Oran1 Ahmed Ben Bella, Algérie. in the host-pathogen interaction would allow to 2SCTMR, EPSP Essenia, Oran, Algérie. evaluate more precisely the role of the genetic 3Laboratoire AQUABIOR, Université Oran1 Ahmed Ben component in susceptibility to tuberculosis. Bella. *[email protected] Keywords: Tuberculosis; IL10 -1082 G/A; Genetic Susceptibility; Immunity. Abstract Tuberculosis remains the leading cause of death P217 due to a single infectious agent in the world with more than 10 million deaths in 2018[1]. Absence of Y-chromosome microdeletions in Although the exogenous risk factors associated patients with Hypospadias with Chordee in with this disease are commonly known, some Eastern Algeria endogenous factors such as susceptibility genetics are much less explored. Many Rania Laouar1, Djalila Rezgoune-Chellat1, Souhem polymorphisms within genes involved in Touabti2, Zoubir Atrih3, Hichem Choutri3, Karima Sifi4, antituberculous immunity such as cytokines have Noureddine Abadi4, Dalila Satta1 been associated with infection in many 1Laboratory of Biology and Molecular Genetics, Department populations[2].This study is the first to analyze of Animal Biology, Faculty of Natural and Life Science, the link between the IL10-1082 G/A 2University Of Mentouri, Constantine, 25000, Algeria. polymorphism and the occurrence of active 3Pediatric Surgery Department Specialized Mother and Child tuberculosis (pulmonary and extra-pulmonary) Hospital –El Eulma-, Setif, 19000, Algeria. 4Pediatric Surgery Department Specialized Mother and Child in a sample of a Western Algerian population. Hospital –El Mansourah-, Constantine, 25000, Algeria. 114 patients diagnosed with active tuberculosis 5Laboratory of Biology and Molecular Genetics, Faculty of (56 Pulmonary, 58 Extrapulmonary) were Medicine, University Constantine 3, Constantine, 25000, enrolled in this study and 48 healthy blood Algeria. donors were recruited as. The DNA extraction *[email protected] was carried out according to a "Salting Out" method and quantified by a spectrophotometric Abstract method (Nanodrop®). Genotyping was The most common external male genital performed using a specific allele PCR technique malformation is hypospadias, affecting 1/125 to (ASO-PCR). The sex ratio was very significantly 1/300 live male births. Hypospadias is defined as different (p<10-6) depending on the location of the abnormal closure of the genital folds during the infection with a predominance of extra- gestational weeks 8–14; it results in a urethral pulmonary forms in women (70.7%) and meatus on the ventral surface of the genital pulmonary form in men (75%). The ganglionic tubercle [1]. The human Y chromosome is form was the most common form among EPTs essential for human sex determination and (49.2%). Allelic and genotypic frequencies of the spermatogenesis. The long arm contains the polymorphism analysis showed very significant azoospermia factor (AZF) region. Microdeletions differences: (p=0.001) and (p=0.003) in this region are responsible for male infertility respectively. Indeed, the A allele was [2]. Few studies have investigated the predominant in patients (59.3% vs 39.6%) with relationship between microdeletions and OR=2.22 [1.36-3.63]. By analyzing the different hypospadias in the world [3,4], but no study has

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been established in Algeria. For this we assigned to their biological properties [1-3]. The regular as objectives to investigate the possible consumption of polyphenol-rich foods may help relationship between Y chromosome decrease the incidence of cardiovascular microdeletions and hypospadias with chordee in diseases, colon cancer [5], liver disorders, Algerian population. Twenty-six patients with obesity, diabetes,ect. [2-3]. The objective of the different degrees of hypospadias with chordee present study is to determine in vitro antioxidant operated and treated at the pediatric surgery activity and phytochemical composition of two department specialized mother and child –El varieties of spinach : wild spinach (Chenopodium Eulma –and at the pediatric surgery department bonus-henricus L.) and grown spinach ( Spinacia specialized mother and child –El Mansourah- oleracea L.). In this context we evaluate in leaves were screened for Y chromosome and stems, the levels of total polyphenols, microdeletions. Genomic DNA was isolated from antioxidant capacity and phytochemical analyses. blood and polymerase chain reaction was carried The determination of total polyphenols was out with a set of 3 STS markers (sY84, sY134 and performed by the spectrophotometric method of sY254) to detect the microdeletions as Folin-Ciocalteu. The antioxidant capacity of the recommended by the European Academy of samples by determining the ferric reducing Andrology. As well as, SRY272 was used as antioxidant power (FRAP) and the effect of internal control. All patients showed normal radical scavenger DPPH. The phytochemical length amplifications for each of the regions identification of spinach is determined by means evaluated, suggesting that microdeletions of the of a reaction of characterization and by thin layer Y chromosome are not a frequent cause chromatography (TLC). Our results showed an hypospadias with chordee. This finding is very important antioxidant activity in both spinach similar to those of Tateno et al.[3] reported that varieties, especially in the wild variety. Total no patient with isolated hypospadias lacked any polyphenols were most abundant in the of 33 DNA loci studied. The same result was Chenopodium bonus-henricus variety than found by Castro et al. [4]. This fact suggests that Spinacia oleracea and in leaves (105.5 ± 5.1 vs patients with hypospadias with chordee are 91.7 ± 3,0 mg/mL) than stems (37,25 ± 1,67vs rarely associated with genetic alterations. It 34,01 ± 1,71) . Moreover, DPPH radical reducing would be more interesting to analyze other STS power was more important in wild leaves markers with larger samples. compared to grown leaves with inhibition percentages of (96.66% vs 90%), this activity of Keywords: Hypospadias; Microdeletions; Y- the extracts may be attributed to its strong chromosome. proton donating ability also antioxidant activity

and radical of the plant is due to the presence of phenolics compounds [4]. Furthermore, P218 phytochemical screenings according to Antioxidant activity and phytochemical characterization reactions and thin layer chromatography, indicated that the two spinach composition of two varieties of spinach varieties contained widely an antioxidant molecules (saponins, anthocyanins, coumarins, Hayet Cherrad1,*, Abdesselem Si Mohammed tannins, quinones, alkaloids and terpenoids). In 1Laboratoire de Nutrition Clinique et Métabolique, conclusion, thanks to their antioxidant power, Département de Biologie, Faculté des Sciences de la Nature spinach is a food particularly valuable for the et de la Vie. Université d’Oran 1, Ahmed Benbella, 31100 prevention of various pathologies. Further Oran, Algérie. studies will be considered to test the spinach 2Centre universitaire Ahmed ZABANA de Relizan. *[email protected] extracts on animal models to confirm their antioxydant activity and their capacity to reduce pathologies damages. Abstract Keywords: Antioxydant; DPPH; Polyphenols Phytochemicals are a diverse, bioactive, and pervasive category of plant secondary metabolites that comprise an essential part of the human diet and are of considerable interest due

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P219 NETgroup which weighed 36.64 ± 1.38 g. A decrease in blood glucose in TESTO-NET (0.84 ± Experiments on Urtica dioica roots hydro- 0.13 g / L) was observed compared to TESTO alcoholic extract activities against benign (1.09 ± 0.32 g / L). Prostate-specific antigen prostatic hypertrophy-induced in mice by (PSA) was increased in TESTO (0.25 ng / mL) testosterone and a hyper-caloric diet. Phyto- and decreased in TESTO-NET (0.18 ng / mL). In chemical, Biological & Histological Studies TESTO, prostatic glands and fibro-muscular tissue exhibited an enlarged appearance whereas animals treated with nettle extract had narrower Abdelkrim Berroukche1,*, Farouk Boudou2, glands and acini of regular shape. Urticadioica Belkacem Belatbi3, Mohamed Terras1, Hanane has a preventive effect in mice with BPH Mebarki1, Khadidja Kerache1, Wassila Lansari4, Imane probably acting by an antioxidative defense Zerarki4, El-Hadj Fidah4 system.

1Research Laboratory of Water Resources & Environment, Keywords: Benign prostatic hypertrophy; Biology Department, Faculty of Science, DrTahar-Moulay University of Saida, Algeria. Urticadioica; Testosterone; Hypercaloric diet; 2Research Laboratory of Environment & Health, University prostate specific antigen Hospital of Sidi-Bel-Abbes, Djillali-Liabes University of Sidi- Bel-Abbes, Algeria. 3Laboratory of Anatomo-Pathology, DrBenzerdjeb Hospital of AinTemouchent, Algeria. 4Research Laboratory of Bio-Toxicology, Pharmacognosy& Biological Valorization of Plants, Biology Department, Faculty of Science, DrTahar-Moulay University of Saida, Algeria. *[email protected]

Abstract Benign prostatic hypertrophy (BPH) is one of the most common pathologies affecting the urogenital system of the elderly man[1].Urticadioica or nettle, a plant rich in antioxidants, is used in traditional medicine to treat the BPH urinary symptoms[2]. This study aimed to assess the hydroalcoholic extract of nettle root (NET) against BPH induced in mice by testosterone and a high calorie diet. Antioxidant activity of nettle root extract and polyphenol assay were performed. In vivo experimentation included biochemical assays and histological study of the prostate gland. A population of 16 male mice divided into 4 groups; a control group (CON), a group (TESTO) receiving an intraperitoneal injection of testosterone (0.5 mL / week) and a high calorie diet, group (TESTO- NET) receiving hormone and 0.2 mL of hydroalcoholic extract of the nettle roots (14.8 mg / mL) and hypercaloric diet and a group (NET) receiving the extract of nettle. The inhibition rate and the polyphenol concentration were respectively 21.3% and 60.3 μg / mL gallic acid. The body weight of the mice (TESTO) was 41.15 ± 1.62 g, in contrast to the TESTO-

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