AC 27/2/13 Item no. 4.67
UNIVERSITY OF MUMBAI
Syllabus for Sem III & IV
Program: M.Sc.
Course: Biotec hnology
(Credit Based Semester and Grad ing System with effect M.Sc.
BIOTECHNOLOGY
SEMESTER- III Course Code UNIT PTC and ATC Credits L / week Introduction to primary and secondary metabolism, important pathways leading to biosynthesis of secondary metabolites in plants, Metabolic products produced from in vitro culturing of plant cells, selection of plant cells/ tissues for production of a specific products, I culture system in secondary plant 1 product, Biosynthesis- batch, continuous cultures, immobilized plant cell, Biotransformation of precursors by cell culturing, metabolic engineering for production of secondary metabolites, Hairy root culture, PSBT301 elicitation 4 Cryopreservation -Principle and types. II Germplasm conservation, Transgenic 1 plants-Edible vaccine, Golden rice Biology of cultured cells Culture vessels, Culture Media, III Microbial contamination, cross 1 contamination. Cryopreservation Primary culture: Types, isolation of tissues, culturing of different cells. Cell lines: Development, Subculture and propagation, immortalization of IV cell line, cell line designation, 1 selection of cell lines, routine maintenance, Cytotoxicity Transformation Culture of tumor cells Course Code UNIT Medical microbiology Credits L / week PSBT302 I Chromosomal disorders- 4 1
2 Karyotyping, G banding, Chromosome analysis, variations, chromosome painting Infections of Respiratory tract- Pneumonia,Tuberculosis. II Nosocomial- Psuedomonas. 1 Viral infections-HIV, Hepatitis. Fungal-Candidiasis Molecular diagnostics for Pneumonia, III Tuberculosis, Pseudomonas, HIV, 1 Hepatitis. Candidiasis IV Biofilms in medicine 1
Course Code UNIT Clinical Studies Credits L / week
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Course Code UNIT Developmental Biology Credits L / week Human Embryonic development: Events during fertilization, in-vitro PSBT304 I fertilization, Zonapellucidaa, 4 1 glycoprotein, Oelemma protein and their role in fertilization, sperm 3 antigens and their functional significance. Molecular and biochemical events during sperm function Post fertilization events: early embryonic development, establishing II multi-cellularity, formation of 1 blastula, embryonic germ layer, tracking of migrating cells. Molecular mechanism of sex hormone action and regulation of gene expression. Implantation and III endometrium antigens involved in 1 implantation. Immunology of pregnancy. Superovulation, embryo culture and embryo transfer technology. Infertility and reproductive vaccines. Frontiers in contraceptive research. IV Cryopreservation of sex gametes and 1 embryos. Ethical issues related to embryo research.
PSBTP301 PTC and ATC 2 4 PSBTP302 Medical microbiology 2 4 PSBTP303 Clinical Studies 2 4 PSBTP304 Developmental Biology 2 4
4 Practicals for Semester III
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1 PTC Media preparation Seed sterilization, Callus induction Isolation and induction of anther cultures using hibiscus Protoplast isolation Somatic embryogenesis
2 ATC Trypsinization, Staining of animal cells
-Monolayer formation (fibroblast) To assay the radical scavenging activity of a tissue hydrolysate -DPPH method
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3 Toxicology - MTT assay
4 Study and present a published clinical case report
5 Medical diagnostic – Identification of organisms from specimens (Multiple drug resistant S. aureus, Pseudomonas spps, Klebsiella pneumoniae, E. coli). Staining of Biofilms
Candling, Observing chick embryo- stages of development; 6 prepared slides/ preserved speciemesn
e el mental i l g isit t la rat r / video lectures for latest 7. developments in the field. To be documented
5 SEMESTER IV
Course Code UNIT Nanotechnology Credits L / Week Introduction, synthesis of nanomaterials, biological methods, use of microbial system & plant extracts, use of proteins & templates like DNA. I Characterization of nanomaterials, 1 analysis techniques, properties of nanomechanical, optical, magnetic properties, electrical conductivity, thermal conductivity.
PSBT401 Carbon nanotubes 4 II Nanorobotiocs devices of nature: 1 ATP synthese, the kinen, myosin, dynein, flagella modulated motion. Nanomedicine: biopharmaceutics, implantable materials, implantable chemicals, surgical aids, III diagnostic tools, nanosensors, 1 nano scanning, nano enabled drug delivery system, nanorobotics in medicine. Application of nanomaterials in IV food,cosmetics, agriculture, 1 environment management Course Code UNIT GMO and environment Credits L / Week
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Solid waste treatment, pollution indicators & biosensors 1 III biodegradation of xenobiotics, pesticides, phytoremediation
Biodegration of waste from food, textile, petrochem, paper IV industries, biological 1 detoxification, Removal of oil spillage & grease deposits Course Code UNIT Bioinformatics Credits L / Week Organization of biological data, databases (raw and processed), I Quering in data bases. 1 Primers in biology(Designing of primers, kinds of primers) Gene finding, motif finding and multiple sequence alignment. PSBT403 4 II Protein sequence analysis (theory 1 and algorithms) Protein structure analysis and applications. Gene expression profiling and its III applications. 1 Microarray technology and basics. Microarray analysis and
7 organization of data Human genome analysis Proteomics. Exploration of data bases, retrieval IV of desired data, BLAST etc. 1 Gene clusters and fusions, consensus sequences, exon intron finder, sequence logo. Course Code UNIT Biostatistics Credits L / Week Statistical population, sample from population, Random sample. I Central Tendency: Mean, Median 1 and Mode, Standard Deviation Confidence intervals Gaussian Distribution and testing for normality, Non-parametric II tests (Sign test, Wilcoxon test, 1 Mann-Whitney Test, Krushkal- Whllis test,), transforming data to create Gaussian Distribution Test of Significance. Hypothesis testing:- Theory of PSBT404 4 errors- Type I and Type II errors, III Null hypothesis, P values-one v/s 1 two tail P values, t-test(paired & unpaired), z-test, Chi square test, contingency table. Comparing three or more groups- Introduction to ANOVA, One way ANOVA, repeated measures ANOVA, Friedman Test. IV 1 Correlation and Regression: Linear and multiple Correlation and Regression.
8 Semester IV Practicals t is mandat r r st dents t nderg ands n Project training in a esta lished la rat r for 6 months; his sh ld inv lve ne r m re relavant instr mentati n techni e hesis n the same t e eval ated the g ide alternativel internal e aminer r ased n the st dents er rmace ritten matter and e erimentati n certi icate mar list t e a ended ith the thesis ternal e aminer t assess r as a Presentation d ring ractical e ams Marks alloted by nternal examnier ld e scaled d n if required as per university guidelines.
Sr No. Experiment
1 Multiple alignment - Phylogenetic tree
2 BLAST - orthologs and paralogs , homologs
3 Motif finding
4 KEGG
5 Structure of proteins - identification of chains helices, special groups, metal ions etc. CATH / SCOP classification of a given protein
6 /BOPQBSUJDMFTTZOUIFTJTDIFNJDBMBOECJPMPHJDBM NFUIPET 4QFDUSPTDPQJDBOBMZTJT 7 Bioremediation- isolation of metal tolerant organisms & study their growth characteristics and pattern
8 Composting – physical & chemical parameters
alidati n - kit based/ demo
PSBTP401 Nanotechnology 2 4 PSBTP402 and environment 2 4 PSBTP403 Bioinformatics 2 4 PSBTP404 Biostatistics 2 4
9 References
Sr No Title of the Book Author Publisher
1 A Introduction to Biostatistics (Second N. Gurumani M J P Publishers Edition‐2005)
2 Basic Biostatistics (2008) B. Burt Gerstman Jones and Bartlett Publishers
3 Biostatistics: Afoundation For Analysis In Wayne W. Daniel John Wiley & Sons Inc. Health Sciences ( 7th Edition 1999)
4 Fundamentals of Biostatistics (2006) Veer BalaRastogi Ane Books India
5 Biostatistics‐ The Bare Essentials ( Second NosmanStreiner B. C. Decker Inc. Edition 2000)
6 Computer Based Decision Making in E. A. Shortifile American Elsevier Medicine
7 Bioinformatics : Sequence and Genome David W. Mount ColdspringHarbor Analysis (Second Edition 2004) Laboratory Press
8 Bioinformatics and Functional Genomics ( Jonathan Pevsner John Wiley & Sons 2003 ) Publications
Sr No Title of the Book Author Publisher
1 Plant Cells in liquid culture (1991) Payne Shuler Hanser Publishers
2 Culture of Animal Cells : A Manual Of Basic R. Ian Freshney Wiley‐Liss Techniques ( 4TH Edition, 2000)
3 Principles and Practice of Animal Tissue SudhaGangal Universities Press Culture (2007)
4 Langman’s Medical Embryology ( 9th Edition T. W. Sadler. Lippincott Williams & 2004) Wilkins
5 Essential Developemental Biology (2nd J. M. W. Slack Blackwell Publishing Edition 2006)
10 6 Developemental Biology (8th Edition 2006) Scott F. Gilbert Sinauer Associates, Inc.
7 The Nanoscopeencyclopedia of Dr.ParagDiwan and Pentagon Press New nanoscience and nanochehnology, Vol. I AshishBharadwaj Delhi (2005)
8 The Nanoscopeencyclopedia of Dr.ParagDiwan and Pentagon Press New nanoscience and nanochehnology, Vol V AshishBharadwaj Delhi (2005)
9 The Nanoscopeencyclopedia of Dr.ParagDiwan and Pentagon Press New nanoscience and nanochehnology, Vol VI AshishBharadwaj Delhi (2005)
10 Nano forms of carbon and its applications Prof.Maheshwar Manad Nanotech Pvt. (2007) Sharon and Dr.Madhuri Ltd. Sharon
11 Biotechnanotechnology lessons from David Goodsell Wiley‐Liss A John Wiley Nature (2004) and sons
12 Nanotechnology‐ Basic science and WillsonKannangava, Oversease Press emerging technologies (2005) Smith, Simmons, Raguse
13 Texbook of Biotechnology (2005) R. C. Dubey S. Chand and Co.
14 Nanotechnology‐ Principles and practices S. K. Kulkarni Capital Publishing Co.
Sr No Title of the Book Author Publisher
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