CRYOPRESERVATION Cryoprotectant Chemicals An integral part of cell culture involves the successful preservation of im- portant cell lines. A primary factor for success is developing an appropri- DIMETHYL SULFOXIDE 25 ml 8.90 196055 100 ml 15.40 ate protocol for a particular cell line. A common misconception among RT [67-68-5] (DMSO) 500 ml 27.40 scientists is that preserving methods for a given cell type are transfer- Cell Culture Reagent 1 liter 45.60 able to similar cell types. Although, this may be true on occassion, it is Purity: ≥99% far from the general rule. Protocols require modification for each cell Ideal for use as a cryoprotectant. NOT FOR HUMAN USE! type. Another key factor for successful preservation is the use of an ef- C2H6SO MW 78.13 fective cryoprotectant. The discovery of glycerol as a preservation me- ETHYLENE GLYCOL 250 ml 10.80 dium led to the rapid discovery of other protectants such as methanol, 151089 1 liter 22.50 RT [107-21-1] ethylene glycol, and DMSO (dimethyl sulfoxide). Purity: 99% min. 1 ml = approx. 1.11 g With any cryopreservation process, the primary objective is maximum C2H6O2 MW 62.1 recovery with minimal damage to the cells. The absence of an effective D-(+)-GLUCOSE 100 g 15.00 cryoprotectant leads to the formation of intracellular ice crystals which 194672 1 kg 23.50 RT [50-99-7] puncture organelles and cell membranes. Also, as water within the me- (Dextrose; Corn sugar) 5 kg 94.00 dium freezes, cells become dehydrated altering the salt concentration Cell Culture Reagent Anhydrous and causing morphological or biological changes after recovery. By us- Crystalline ing a proven cryoprotectant and controlling the rate of temperature C6H12O6 MW 180.2 change, cell loss is minimized and cell recovery is optimized. GLYCEROL 100 ml 31.40 194680 500 ml 43.00 An often overlooked aspect of serum-free cell culture is the presence of RT [56-81-5] Cell Culture Reagent both serum and DMSO in traditional cryopreservation media. ICN’s se- Highly purified rum-free, DMSO-free preservation medium, Cellvation, eliminates the Purity: 99+% cytotoxic risks and extends the amount of time cells can be exposed to Ideal for use as a cryoprotectant. C H O MW 92.09 the cryoprotectant while avoiding subcellular biochemical changes and 3 8 3 differentiation artifacts. Additionally, the integrity of the defined serum- D-MANNITOL, ACS 250 g 15.70 152540 [69-65-8] 500 g 26.20 free environment is maintained and incidential exposure to serum is RT ACS Reagent Grade 1 kg 40.30 eliminated. It is equally effective for serum-supplemented cultures. C6H14O6 MW 182.2 Alternatively, ICN offers Opti-Freeze, a traditional DMSO, serum METHANOL 500 ml 10.00 based ready-to-use preservation medium that provides optimal recovery 155387 1 liter 18.00 RT [67-56-1] and minimizes the damage associated with freezing and storing cells. Spectrophotometric Grade Purity: 99+% All sterility testing is performed to USP XXIII guidelines, and each lot is 1 ml = approx. 0.79 g. negative for mycoplasma infection. Protection and recovery efficiencies CH3OH MW 32.04 are monitored using three cell types. PHOSPHATE BUFFERED SALINE (1X PBS Liquid) Dulbecco’s Formula Cellvation Cryopreservation Medium Storage temperature: 15-30°C Superior cell recovery and faster cell attachment after thawing 1860049 100 ml 10.20 Ready-to-use preserving medium 1860054 500 ml 13.50 Contains NO serum, DMSO or other cytotoxic agents Eliminates cytotoxic risks associated with DMSO POLYVINYLPYRROLIDONE 100 g 14.25 196056 [9003-39-8] 500 g 53.80 Reduces preparation and centrifugation steps RT ° (PVP 10) Recommended storage - 4-8 C Average MW 10,000 K Value (intrinsic viscosity): 12-18 Product Catalog No. Quantity Prepared for use as a cryoprotectant. Also, it is well suited for Cellvation 2030046 20 ml plant cell culture. 20300M2 60 ml POLYVINYLPYRROLIDONE 100 g 11.30 102787 500 g 32.70 RT [9003-39-8] Opti-Freeze DMSO Preservation Medium (PVP K 90) 1 kg 57.10 MW AVERAGE 360,000 5 kg 243.65 Ready-to-use preserving medium that contains serum and DMSO Maximizes recovery while minimizing cell damage PROPYLENE GLYCOL 500 ml 11.30 Sterile filtered through a capsule filter of 0.2 µm 151957 1 liter 18.20 RT [57-55-6] Reduces cytotoxic risks associated with DMSO (1,2-Propanediol) Saves time and reduces expense 1 ml = approx. 1.04 g Endotoxin - <1 ng/ml determined by LAL assay C3H8O2 MW 76.1 Thoroughly tested for sterility and performance Recommended storage: -20°; stable for a minimum of 3 years SUCROSE 500 g 24.80 194747 1 kg 34.45 RT [57-50-1] Cell Culture Reagent 5 kg 109.15 Product Catalog No. Quantity Crystalline  Opti-Freeze 2030148 50 ml C12H22O11 MW 342.3

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