HEPES Sodium Salt Specification Comparison

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● Cell Biology Sigma-Aldrich® HEPES Sodium Salt Physical Properties ● Cell Culture 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid ● Core Bioreagents sodium salt Synonyms N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) �❍ Learning Center sodium salt

�❍ Biological Buffers Molecular C8H17N2O4SNa MDL Number MFCD00036463 Useful pH range 6.8 - 8.2 Formula ■ Learning Center Molecular Weight 260.29 EG/EC Number 278-169-7 pKa (at 25) 7.5 CAS Number 75277-39-3 ■ Biological Buffer Products Sigma-Aldrich HEPES Sodium Salt Specifications Comparison H7006 H3784 H8651 ■ BIS-TRIS BioReagent, suitable for cell ■ BIS-TRIS Propane Grade ≥99.5% (titration) culture, BioXtra, ≥99.5% (titration) ≥99.5% (titration) ■ HEPES Purity ≥99.5% ≥99.5% ≥99.5%

■ HEPES Sodium Reagent Testing H7006 H3784 H8651 Salt Enhanced Quality X X X ■ MES Profile Buffers 290NM ≤0.05 ≤0.05 ■ MES Sodium Salt 260NM ≤0.05 <0.2 ■ MOPS 280NM ≤0.05 <0.2 HEPES Sodium Salt Specification Comparison IR X X ■ MOPS Sodium 10.0 - 12.0 (20 °C, 1 M in ■ Sodium Chloride pH Salt H2O) http://stlwcmqa01.sial.com/author/life-science/core-bioreagents/biological-buffers/hepes-sodium-salt.html® (1 of 3) [8/13/2010 11:15:44 AM] ■ Trizma HCl Karl Fischer ≤3.0% ≤3.0% <0.1% ■ Trizma® Insoluble Matter Total Impurities ■ Water Life Science Testing ■ S-A BPC H7006 H3784 H8651 Advantage Endotoxin ≤0.1 eu/mg SPC/TAMC ≤20 cfu/g ≤1000 cfu/g ■ Biological Buffers Cell test X Quality System RNAse X DNAse X ■ Convenience Nickase X Packaging Protease X

● Custom Oligos Trace Elemental Impurities H7006 H3784 H8651 ● Epigenetics Ammonia(NH4) <0.05% ● Functional Genomics & RNAi Phosphorus(P) <0.005% ● Metabolomics Chloride(Cl) <0.05%

● Molecular Biology Sulfate(SO4) <0.05%

● Nutrition Research Aluminum(Al) <0.0005%

● Proteomics Arsenic(As) Barium(Ba) ● Stem Cell Biology Bismuth(Bi) ● Transgenics Calcium(Ca) <0.0005%

● Zinc Finger Nuclease (ZFN) Cadmium(Cd) Cobalt(Co) ● Learning Center Chromium(Cr) ● Life Science Services Copper(Cu) <0.0005%

● Labware Iron(Fe) ≤0.0005% <0.0005% Potassium(K) ≤0.005% Lithium(Li) Magnesium(Mg) <0.0005% Manganese(Mn) Molybdenum(Mo) http://stlwcmqa01.sial.com/author/life-science/core-bioreagents/biological-buffers/hepes-sodium-salt.html (2 of 3) [8/13/2010 11:15:44 AM] HEPES Sodium Salt Specification Comparison

10.0 - 12.0 (20 °C, 1 M in ■ Sodium Chloride pH H2O) ® ■ Trizma HCl Karl Fischer ≤3.0% ≤3.0% <0.1% ■ Trizma® Insoluble Matter Total Impurities ■ Water Life Science Testing ■ S-A BPC H7006 H3784 H8651 Advantage Endotoxin ≤0.1 eu/mg SPC/TAMC ≤20 cfu/g ≤1000 cfu/g ■ Biological Buffers Cell test X Quality System RNAse X DNAse X ■ Convenience Nickase X Packaging Protease X

● Custom Oligos Trace Elemental Impurities H7006 H3784 H8651 ● Epigenetics Ammonia(NH4) <0.05% ● Functional Genomics & RNAi Phosphorus(P) <0.005% ● Metabolomics Chloride(Cl) <0.05%

● Molecular Biology Sulfate(SO4) <0.05%

● Nutrition Research Aluminum(Al) <0.0005%

● Proteomics Arsenic(As) Barium(Ba) ● Stem Cell Biology Bismuth(Bi) ● Transgenics Calcium(Ca) <0.0005%

● Zinc Finger Nuclease (ZFN) Cadmium(Cd) Cobalt(Co) ● Learning Center Chromium(Cr) ● Life Science Services Copper(Cu) <0.0005%

● Labware Iron(Fe) ≤0.0005% <0.0005% Potassium(K) ≤0.005% Lithium(Li) Magnesium(Mg) <0.0005% Manganese(Mn) HEPES Sodium Salt Specification Comparison Molybdenum(Mo) http://stlwcmqa01.sial.com/author/life-science/core-bioreagents/biological-buffers/hepes-sodium-salt.htmlNickel(Ni) (2 of 3) [8/13/2010 11:15:44 AM] Lead(Pb) ≤0.0005% <0.0005% <0.001% Strontium(Sr) Zinc(Zn) <0.0005%

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