HEPES Sodium Salt Specification Comparison LoginRegisterChange Country Advanced Products Services Support Custom Products Order Center MSDS | | | | | Search Life Science > Core Bioreagents > Biological Buffers > HEPES Sodium Salt Biological Buffers 3050 Spruce Street Saint Louis, Missouri 63103 USA Telephone (800) 521-8956 • (314) 771-5765 ● Life Science Home Fax (800) 325-5052 • (314) 771-5757 HEPES Sodium Salt sigma-aldrich.com ● Your Favorite Gene - Search Specification Comparison Printer friendly PDF version (117 Kb) ● Life Science Products HEPES Sodium Salt ● Cell Biology Sigma-Aldrich® HEPES Sodium Salt Physical Properties ● Cell Culture 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid ● Core Bioreagents sodium salt Synonyms N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) �❍ Learning Center sodium salt �❍ Biological Buffers Molecular C8H17N2O4SNa MDL Number MFCD00036463 Useful pH range 6.8 - 8.2 Formula ■ Learning Center Molecular Weight 260.29 EG/EC Number 278-169-7 pKa (at 25) 7.5 CAS Number 75277-39-3 ■ Biological Buffer Products Sigma-Aldrich HEPES Sodium Salt Specifications Comparison H7006 H3784 H8651 ■ BIS-TRIS BioReagent, suitable for cell ■ BIS-TRIS Propane Grade ≥99.5% (titration) culture, BioXtra, ≥99.5% (titration) ≥99.5% (titration) ■ HEPES Purity ≥99.5% ≥99.5% ≥99.5% ■ HEPES Sodium Reagent Testing H7006 H3784 H8651 Salt Enhanced Quality X X X ■ MES Profile Buffers 290NM ≤0.05 ≤0.05 ■ MES Sodium Salt 260NM ≤0.05 <0.2 ■ MOPS 280NM ≤0.05 <0.2 HEPES Sodium Salt Specification Comparison IR X X ■ MOPS Sodium 10.0 - 12.0 (20 °C, 1 M in ■ Sodium Chloride pH Salt H2O) http://stlwcmqa01.sial.com/author/life-science/core-bioreagents/biological-buffers/hepes-sodium-salt.html® (1 of 3) [8/13/2010 11:15:44 AM] ■ Trizma HCl Karl Fischer ≤3.0% ≤3.0% <0.1% ■ Trizma® Insoluble Matter Total Impurities ■ Water Life Science Testing ■ S-A BPC H7006 H3784 H8651 Advantage Endotoxin ≤0.1 eu/mg SPC/TAMC ≤20 cfu/g ≤1000 cfu/g ■ Biological Buffers Cell test X Quality System RNAse X DNAse X ■ Convenience Nickase X Packaging Protease X ● Custom Oligos Trace Elemental Impurities H7006 H3784 H8651 ● Epigenetics Ammonia(NH4) <0.05% ● Functional Genomics & RNAi Phosphorus(P) <0.005% ● Metabolomics Chloride(Cl) <0.05% ● Molecular Biology Sulfate(SO4) <0.05% ● Nutrition Research Aluminum(Al) <0.0005% ● Proteomics Arsenic(As) Barium(Ba) ● Stem Cell Biology Bismuth(Bi) ● Transgenics Calcium(Ca) <0.0005% ● Zinc Finger Nuclease (ZFN) Cadmium(Cd) Cobalt(Co) ● Learning Center Chromium(Cr) ● Life Science Services Copper(Cu) <0.0005% ● Labware Iron(Fe) ≤0.0005% <0.0005% Potassium(K) ≤0.005% Lithium(Li) Magnesium(Mg) <0.0005% Manganese(Mn) Molybdenum(Mo) http://stlwcmqa01.sial.com/author/life-science/core-bioreagents/biological-buffers/hepes-sodium-salt.html (2 of 3) [8/13/2010 11:15:44 AM] HEPES Sodium Salt Specification Comparison 10.0 - 12.0 (20 °C, 1 M in ■ Sodium Chloride pH H2O) ® ■ Trizma HCl Karl Fischer ≤3.0% ≤3.0% <0.1% ■ Trizma® Insoluble Matter Total Impurities ■ Water Life Science Testing ■ S-A BPC H7006 H3784 H8651 Advantage Endotoxin ≤0.1 eu/mg SPC/TAMC ≤20 cfu/g ≤1000 cfu/g ■ Biological Buffers Cell test X Quality System RNAse X DNAse X ■ Convenience Nickase X Packaging Protease X ● Custom Oligos Trace Elemental Impurities H7006 H3784 H8651 ● Epigenetics Ammonia(NH4) <0.05% ● Functional Genomics & RNAi Phosphorus(P) <0.005% ● Metabolomics Chloride(Cl) <0.05% ● Molecular Biology Sulfate(SO4) <0.05% ● Nutrition Research Aluminum(Al) <0.0005% ● Proteomics Arsenic(As) Barium(Ba) ● Stem Cell Biology Bismuth(Bi) ● Transgenics Calcium(Ca) <0.0005% ● Zinc Finger Nuclease (ZFN) Cadmium(Cd) Cobalt(Co) ● Learning Center Chromium(Cr) ● Life Science Services Copper(Cu) <0.0005% ● Labware Iron(Fe) ≤0.0005% <0.0005% Potassium(K) ≤0.005% Lithium(Li) Magnesium(Mg) <0.0005% Manganese(Mn) HEPES Sodium Salt Specification Comparison Molybdenum(Mo) http://stlwcmqa01.sial.com/author/life-science/core-bioreagents/biological-buffers/hepes-sodium-salt.htmlNickel(Ni) (2 of 3) [8/13/2010 11:15:44 AM] Lead(Pb) ≤0.0005% <0.0005% <0.001% Strontium(Sr) Zinc(Zn) <0.0005% Copyright © 2010 Sigma-Aldrich Co. Reproduction forbidden without permission. Sigma-Aldrich brand products are sold exclusively through Sigma-Aldrich, Inc. back to top back to Biological Buffers ORDERING SERVICE & SUPPORT CORPORATE ● Site Map ● Site Use Terms ● Custom Products ● Customer Support ● Corporate ● Careers ● Privacy ● eCommerce Solutions ● Technical Service ● Investor Relations ● Events ● Literature Portal ● Order Center ● Web Help Desk ● Business Development ● Programs ● Tool Box ● Products ● MSDS ● Worldwide Offices ● REACH Regulations ● Terms & Conditions ● C of A ● About Us ● Contact Us Copyrights © 2010 Sigma-Aldrich Co. All Rights Reserved. Reproduction of any materials from the site is strictly forbidden without permission. Sigma-Aldrich brand products are sold exclusively through Sigma-Aldrich, Inc. http://stlwcmqa01.sial.com/author/life-science/core-bioreagents/biological-buffers/hepes-sodium-salt.html (3 of 3) [8/13/2010 11:15:44 AM].