MTHFD2 monoclonal antibody (M01J), clone 4G7-2G3
Catalog # : H00010797-M01J 規格 : [ 100 ug ] List All
Specification Application Image
Product Mouse monoclonal antibody raised against a full-length recombinant Western Blot (Cell lysate) Description: MTHFD2.
Immunogen: MTHFD2 (AAH01548, 16 a.a. ~ 344 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence: SLRLRPFHLAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVASGNKRPHL SVILVGENPASHSYVLNKTRAAAVVGINSETIMKPASISEEELLNLINKLNND enlarge DNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVINVGRMCLDQYSML Western Blot (Transfected PATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPG lysate) GDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGIN RVHDPVTAKPKLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTII AAKKVLRLEEREVLKSKELGVATN
Host: Mouse
Human Reactivity: enlarge
Preparation Cell Culture Production Western Blot (Recombinant Method: protein) (CX Grade Antibody List) Immunohistochemistry Isotype: IgG2a Kappa (Formalin/PFA-fixed paraffin- embedded sections) Quality Control Antibody Reactive Against Recombinant Protein. Testing:
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Sandwich ELISA (Recombinant protein)
Western Blot detection against Immunogen (61.93 KDa) . enlarge Storage Buffer: In 1x PBS, pH 7.4 ELISA Storage Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. Instruction:
MSDS: Download
Datasheet: Download
Applications
Western Blot (Cell lysate)
Page 1 of 4 2021/6/15 MTHFD2 monoclonal antibody (M01J), clone 4G7-2G3. Western Blot analysis of MTHFD2 expression in HepG2. Protocol Download
Western Blot (Transfected lysate)
Western Blot analysis of MTHFD2 expression in transfected 293T cell line by MTHFD2 monoclonal antibody (M01J), clone 4G7-2G3.
Lane 1: MTHFD2 transfected lysate (Predicted MW: 26.8 KDa). Lane 2: Non-transfected lysate. Protocol Download
Western Blot (Recombinant protein) Protocol Download
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image Immunoperoxidase of monoclonal antibody to MTHFD2 on formalin-fixed paraffin- embedded human lymphoma. [antibody concentration 3 ug/ml] Protocol Download
Sandwich ELISA (Recombinant protein)
Page 2 of 4 2021/6/15 Detection limit for recombinant GST tagged MTHFD2 is 0.3 ng/ml as a capture antibody. Protocol Download
ELISA
Gene Information
Entrez GeneID: 10797
GeneBank BC001548 Accession#:
Protein AAH01548 Accession#:
Gene Name: MTHFD2
Gene Alias: NMDMC
Gene methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, Description: methenyltetrahydrofolate cyclohydrolase
Omim ID: 604887
Gene Ontology: Hyperlink
Gene Summary: This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme- magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein- coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq
Other NAD-dependent methylene tetrahydrofolate dehydrogenase Designations: cyclohydrolase,methylene tetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase,methylene tetrahydrofolate dehydrogenase 2
Gene Pathway Glyoxylate and dicarboxylate metabolism Metabolic pathways One carbon pool by folate
Related Disease Adenocarcinoma Cleft Lip Cleft Palate Esophageal Neoplasms Genetic Predisposition to Disease Heart Defects, Congenital Lung Neoplasms Lymphoma, Follicular Lymphoma, Large B-Cell, Diffuse Pulmonary Disease, Chronic Obstructive Spinal Dysraphism Urinary Bladder Neoplasms Werner syndrome
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