XXV PVA meeting

CONFERENCE AT A GLANCE Sunday, August 20 2:00 PM Registration 5:00 PM Reception 6:30 PM Dinner 8:00 PM Opening Session Session 1 Keynote I: Sriram Subramaniam, Ph.D., NIH/NCI Keynote II: Alan Davidson, Ph.D., University of Toronto

Monday, August 21 7:30 AM Breakfast 9:00 AM VIRUS ENTRY and EGRESS I Session 2 11:00 AM VIRUS HOST INTERACTION Session 3 12:40 PM Lunch 3:00 PM POSTER SECTION A 6:30 PM Dinner 8:00 PM DNA VIRUS STRUCTURE and ASSEMBLY I Session 4

Tuesday, August 22 7:30 AM Breakfast 9:00 AM GENOME PACKAGING I Session 5 11:00 AM Special Session: THE CRYO-EM REVOLUTION Session 6 12:30 PM Lunch 3:00 PM Free afternoon / evening

Wednesday, August 23 7:30 AM Breakfast 9:00 AM RNA VIRUS STRUCTURE and ASSEMBLY I Session 7 11:00 AM GENOME PACKAGING II Session 8 12:40 PM Lunch 3:00 PM POSTER SECTION B 6:30 PM Dinner 8:00 PM DNA VIRUS STRUCTURE and ASSEMBLY II Session 9

Thursday, August 24 7:30 AM Breakfast 9:00 AM VIRUS ENTRY and EGRESS II Session 10 11:00 AM GENOME PACKAGING III Session 11 12:40 PM Lunch 2:00 PM VIRUS TECHNOLOGY Session 12 3:30 PM DNA VIRUS STRUCTURE and ASSEMBLY III Session 13 5:30 PM Award celebration / PVA Business meeting 6:30 PM Special Dinner

Friday, August 25 9:00-11 AM Departure

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XXV PVA meeting

CONFERENCE PROGRAM

Sunday, August 20, 2017

2:00 PM Registration

5:00 – 6:30 PM Hors D'Oeuvres Reception

6:30 – 8:00 PM Dinner

8:00 PM Opening Session Chair: Michael Rossmann

(45min Talk) Keynote Speaker I: Sriram Subramaniam, Ph.D., NIH/NCI, Bethesda, MD, USA Title: High-resolution 3D electron microscopy: Progress and new opportunities

(45min Talk) Keynote Speaker I: Alan Davidson, Ph.D., University of Toronto, ON, Canada Title: Phages fight back: the functioning and prevalence of anti-CRISPRs

Monday, August 21, 2017

7:30 – 9:00 AM Breakfast

9:00 – 10:40 AM VIRUS ENTRY and EGRESS I Chair: Ann Palmenberg

20min Talk #1 Reza Khayat, Ph.D., City College of New York, NY, USA Title: Mechanism of cellular recognition and entry by porcine circovirus 2

20min Talk #2 Eric May, Ph.D., University of Connecticut, Storrs, CT, USA Title: Modeling Interactions of Lytic Peptides from the Non-Enveloped Flock House Virus Capsid with Biomembranes

20min Talk #3 Ian J. Molineux, Ph.D., the University of Texas at Austin, Austin, TX, USA Title: The initiation of infection by bacteriophage T7

20min Talk #4 Sarah Doore, Ph.D., Michigan State University, East Lansing, MI, USA Title: Isolation of Shigella-infecting phages from Michigan water sources

10min Talk #5 Nicholas Stone, University of Massachusetts, Worcester, MA, USA Title: Elucidating the molecular mechanisms of viral maturation using a thermophilic bacteriophage

10min Talk #6 Amber Vu, University of Iowa, Iowa City, IA, USA Title: Extragenic Suppression of a Mutation in Herpes Simplex Virus 1 UL34 That Affects Lamina Disruption and Nuclear Egress

10:40 – 11:00 AM Coffee Break

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XXV PVA meeting

11:00 – 12:40 PM VIRUS HOST INTERACTION Chair: Paulo Tavares

#1 (20min Talk) David Veesler, Ph.D., University of Washington, Seattle, WA, USA Title: A potent anti-gH/gL neutralizing antibody defines a site of vulnerability on Epstein-Barr virus

#2 (20min Talk) Stefanie Barbirz, Ph.D., University of Potsdam, Potsdam, Germany Title: Host interactions of O antigen specific bacteriophages: Linking tailspike recognition to particle opening

#3 (20min Talk) Sergey Buth, Ph.D., University of Texas Medical Branch, Galveston, TX, USA Title: Exopolysaccharide degrading – specificity determinants of Acinetobacter baumannii phages

#4 (20min Talk) Xiaoli Xiong, Ph.D., University of Washington, Seattle, WA, USA Title: Architecture of a deltacoronavirus spike glycoprotein and its glycan shield

#5 (10min Talk) #5 Nikolai Prokhorov, Ph.D., University of Texas, Galveston, TX, USA Title: Host recognition by podoviruses G7C and Alt

#6 (10min Talk) #6 S. Saif Hasan, Ph.D., , West Lafayette, IN, USA Title: A human antibody against crosslinks the E protein to prevent infection

12:40 – 1:40 PM Lunch

3:00 – 6:00 PM POSTER SECTION A

6:30 – 8:00 PM Dinner

8:00 – 9:40 PM DNA VIRUS STRUCTURE and ASSEMBLY I Chair: Carol Teschke

#1 (20min Talk) Nicola Abrescia, Ph.D., CIC bioGUNE, CIBERehd, Derio, Spain Title: Assembly of vertical single beta-barrel membrane-containing viruses by high-resolution cryo-EM

#2 (20min Talk) Chuan Xiao, Ph.D., University of Texas at El Paso, El Paso, TX, USA Title: Jelly-roll spiraling all the way

#3 (20min Talk) Tina Motwani, Ph.D., University of Connecticut, Storrs, CT, USA Title: Scaffolding protein: An engineer of the portal ring complex in bacteriophage P22

#4 (20min Talk) Jodi Hadden, Ph.D., University of Delaware, Newark, DE, USA Title: Insights into the structure and function of the hepatitis B virus capsid revealed by microsecond all-atom simulation

#5 (10min Talk) Jason Schrad, Michigan State University, East Lansing, MI, USA Title: Staring down Titans: Characterization of the giant Samba virus

#6 (10min Talk) Patricia Campbell, University of Pittsburgh, Pittsburgh, PA, USA Title: In vivo structure of the λ phage tail tube protein

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XXV PVA meeting

Tuesday, August 22, 2017

7:30 – 9:00 AM Breakfast

9:00 – 10:40 AM Shelley Grimes Memorial Session: GENOME PACKAGING I Chair: Paul Jardine

#1 (20min Talk) Paul Jardine, Ph.D., , Minneapolis MN, USA Title: Progress toward a complete atomic-resolution structure of bacteriophage phi29

#2 (20min Talk) Paulo Tavares, Ph.D., CNRS, Gif-sur-Yvette, France Title: The bacteriophage SPP1 portal protein headful sensor mechanism

#3 (20min Talk) Michael Feiss, Ph.D., University of Iowa, Iowa City IA, USA Title: DNA Recognition and Cleavage in the -Like Phages

#4 (20min Talk) Peixuan Guo, Ph.D., The Ohio State University, Columbus, Ohio, USA. Title: Motion direction control of revolving biomotors utilizing the channel chirality and arginine finger of the asymmetrical hexameric ATPase ring

#5 (10min Talk) Siying Lin, The Catholic University of America, Washington, DC, USA Title: Altering the speed of a DNA packaging motor from bacteriophage T4

#6 (10min Talk) Herman Fung, Department of Biology, , York, UK Title: The bacteriophage HK97 DNA packaging motor: structural and single-molecule biophysical analysis

10:40 – 11:00 AM Coffee Break

11:00 – 12:30 PM Special Session: THE CRYO-EM REVOLUTION (sponsored by FEI) Chair: James Conway

#1 (30min Talk) Alasdair Steven, Ph.D., Principal Scientist, NIH/ NIAMS, Bethesda, MD, USA Title: Heterogeneity and Disorder and the Constraints Imposed in Cryo-EM of Viruses and Virus- related Specimens

#2 (30min Talk) Hong Zhou, Ph.D., Principal Investigator, UCLA, USA Title: Confronting challenges in high-resolution cryo-EM: viral genomes and huge viruses

#3 (30min Talk) Jeffrey Lengyel, Ph.D., Principal Scientist, Life Sciences, FEI Title: Recent technological developments in cryo-EM: Advancing challenging structural biology research problems

12:30 – 1:30 PM Lunch

Free afternoon / evening

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XXV PVA meeting

Wednesday, August 23, 2017

7:30 – 9:00 AM Breakfast

9:00 – 10:40 AM RNA VIRUS STRUCTURE and ASSEMBLY Chair: David Veesler

#1 (20min Talk) Michael Rossmann, Ph.D., Purdue University, West Lafayette, IN, USA Title: The structure of Zika virus

#2 (20min Talk) Junjie Zhang, Ph.D., Texas A&M University, College Station, TX, USA Title: Structures of the single-stranded RNA virus Qβ reveal its MurA-binding interface and internalized coat proteins

#3 (20min Talk) Xinghong Dai, Ph.D., UCLA, Los Angeles, CA, USA Title: In situ structures of the genome and genome-delivery apparatus in ssRNA phage MS2

#4 (20min Talk) Peter Stockley, Ph.D., University of Leeds, Leeds, UK Title: ’s ssRNA Self-assembly Instruction Manual: The Roles and Consequences of Packaging Signal-Mediated Assembly

#5 (10min Talk) Lindsey Organtini, Pennsylvania State University, Hershey, PA, USA Title: Cryo-EM structures of honey bee deformed wing virus reveal conformational changes linked to genome release

#6 (10min Talk) Jason Pattis, University of Connecticut, Storrs, CT, USA Title: Long Timescale Simulations of Monomeric Lassa Virus Nucleoprotein Reveals Insights into Its RNA Binding Mechanism and Ribonucleoprotein Contacts

10:40 – 11:00 AM Coffee Break

11:00 – 12:40 PM GENOME PACKAGING II Chair: Doug Smith

#1 (20min Talk) Stephen Harvey, Ph.D., University of Pennsylvania, Philadelphia, PA, USA Title: Electrostatically-Driven DNA Conformational Changes Drive Genome Packaging in dsDNA Bacteriophages: Evidence from Computational and Theoretical Studies

#2 (20min Talk) Susan Schroeder Ph.D., University of Oklahoma, Norman, OK, USA Title: NMR structures and dynamics in a prohead RNA loop that binds metal ions

#3 (20min Talk) Peng Jing, Ph.D., Indiana-Purdue University, Fort Wayne, IN, USA Title: The Flashing Brownian Ratchet in the Connector at the Late Stage of DNA Packaging in the Bacteriophage Phi29

#4 (20min Talk) Allen Eastlund, Ph.D., University of Minnesota, Minneapolis, MN, USA Title: Disruption of a pentameric ring motor through molecular and substrate substitution

#5 (10min Talk) Wei-Chun Tang, the Catholic University of America, Washington, DC, USA Title: The bacteriophage T4 portal is a signal transducer of the DNA packaging machine

#6 (10min Talk) Eva Weiss, University of York, York, UK

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XXV PVA meeting

Title: Phylogeny of viral strains based on packaging signal profiles

12:40 – 1:40 PM Lunch

3:00 – 6:00 PM POSTER SECTION B

6:30 – 8:00 PM Dinner

8:00 – 9:40 PM DNA VIRUS STRUCTURE and ASSEMBLY II Chair: Susan Hafenstein

#1 (20min Talk) Cécile Breyton, Ph.D., Institut de Biologie Structurale, Grenoble, France Title: Bacteriophage T5 tail tube structure elucidates the trigger for Siphoviridae DNA ejection

#2 (20min Talk) Mihnea Bostina, Ph.D., University of Otago, Dunedin, New Zealand Title: Insight into Assembly Mechanism or Iridoviruses

#3 (20min Talk) Alexis Huet, Ph.D., University of Pittsburgh, Pittsburgh, PA, USA Title: Expansion of the capsid of bacteriophage T5: a high-resolution study of a highly dynamic process

#4 (20min Talk) Robert Duda, Ph.D., University of Pittsburgh, Pittsburgh, PA, USA Title: A model for the early steps of HK97 capsid assembly

#5 (10min Talk) Sebastian Leptihn, Ph.D., University of Hohenheim, Stuttgart, Germany Title: Filamentous Phage Assembly

#6 (10min Talk) Olesia Ivantsiv, University of Toronto, Toronto, ON, Canada Title: This chaperone can dance – a new role for phage tail fibre assembly proteins

Thursday, 24 August, 2017

7:30 – 9:00 AM Breakfast

9:00 – 10:40 AM VIRUS ENTRY and EGRESS II Chair: Peter Stockley

#1 (20min Talk) Sherwood Casjens, Ph.D., University of Utah, Salt Lake City, UT, USA Title: Phage P22 DNA Delivery into Susceptible Cells

#2 (20min Talk) William Newcomb, NIH/ NIAMS, Bethesda, MD, USA Title: Structure of the Primary Enveloped Virion of Herpes Simplex Virus type 1, a transient intermediate in the nuclear egress pathway

#3 (20min Talk) Veronique Taylor, Ph.D., University of Toronto, Toronto ON, CA Title: JBD26-GP61, a conserved moron, promotes phage resistance by disrupting cyclic di-GMP mediated type-iv pilus extension

#4 (20min Talk) Altaira Dearborn, Ph.D., NIH/NIAMS, Bethesda, MD, USA Title: Crystal structure of HIV-1 Rev with an RNA aptamer

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XXV PVA meeting

#5 (10min Talk) Alec Fraser, University of Texas Medical Branch, Galveston, TX, USA Title: Energetics of sheath contraction in contractile injection systems

#6 (10min Talk) Aaron Roznowski, University of Arizona, Tucson, AZ, USA Title: Mutagenic analysis of a DNA translocating tube’s interior surface

10:40 – 11:00 AM Coffee Break

11:00 – 12:40 PM GENOME PACKAGING III Chair: Fred Antson

#1 (20min Talk) Douglas Smith, Ph.D., UCSD, La Jolla, CA, USA Title: Insights on viral DNA packaging motor mechanisms from the effects of motor residue changes on single-molecule packaging dynamics

#2 (20min Talk) Emma Wroblewski, University of Leeds, Leeds, UK Title: Rewriting Nature’s ssRNA Viral Assembly Manual

#3 (20min Talk) Phil Serwer, Ph.D., the University of Texas Health Center, San Antonio, TX, USA Title: Directed Evolution-Driven Tests of the Assumption of Shell Inertness during Phage T3 DNA Packaging

#4 (20min Talk) Ruigang Xu, University of York, York, UK Title: Structure of the large terminase from a hyperthermophilic virus and its mechanistic implications

#5 (10min Talk) Jean Sippy, University of Iowa, Iowa City, IA, USA Title: Identification of Residues in the Endonuclease Domain of ’s TerL with a Potential Role in Metal-Binding Catalysis

#6 (10min Talk) Serban Ilca, University of Oxford, Oxford, UK Title: The Spooled Organization of the Bacteriophage 6 Genome Revealed by Cryo-Electron Microscopy

12:40 – 1:40 PM Lunch

2:00 – 3:30 PM VIRUS TECHNOLOGY Chair: Karen Maxwell

#1 (20min Talk) Audray Harris, Ph.D., NIH/NIAID, Bethesda, MD, USA Title: Cryo-electron microscopy of nanoparticles for influenza virus vaccine development

#2 (20min Talk) Pan Tao, Ph.D., the Catholic University of America, Washington, DC, USA Title: Bacteriophage T4 nanoparticle as a platform for rapid production of effective subunit vaccines

#3 (20min Talk) Alexandra Alimova, Ph.D., City University School of Medicine, CUNY, NY, USA Title: Zika virus-like particle (VLP) based vaccine

#4 (20min Talk) Juan Perilla, Ph.D., University of Delaware, Newark, DE, USA Title: Biophysical characterization of HIV-1 virus like particles

#5 (10min Talk) Alexis Catala, University of Colorado Denver, Aurora, CO, USA Title: Multifunctional Nanostructures for Targeted Cancer Therapeutics Utilizing Bacteriophage Lambda

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XXV PVA meeting

3:30 – 4:00 PM Coffee Break

4:00 – 5:30 PM DNA VIRUS STRUCTURE and ASSEMBLY III Chair: Petr Leiman

#1 (20min Talk) Elena Orlova, Ph.D., Birkbeck College, London, UK Title: Conformational changes in the capsid protein during maturation of phage SPP1

#2 (20min Talk) Ben Fane, Ph.D., University of Arizona, Tucson, AZ, USA Title: Altering the flux of assembly intermediates through the øX174 assembly pathway

#3 (20min Talk) James Conway, Ph.D., University of Pittsburgh, Pittsburgh, PA, USA Title: Capsids and Genomes of Jumbo-Sized Bacteriophages Reveal the Evolutionary Reach of the HK97 fold

#4 (20min Talk) Andrei Fokine, Ph.D., Purdue University, West Lafayette, IN, USA Title: Structure and activation mechanism of the bacteriophage T4 capsid assembly protease

#5 (10min Talk) Christopher Schlicksup, Indiana University, Bloomington, IN, USA Title: Cryo-EM structures of Hepatitis B Virus capsids in complex with a fluorescent derivative of an antiviral compound

5:30 PM Award celebration for “Best Talk / Poster “

6:00 PM PVA Business meeting

6:30 – 8:00 PM Special Dinner

Party-time

Friday, August 25, 2017

9:00-11 AM Departure

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