XXV PVA meeting CONFERENCE AT A GLANCE Sunday, August 20 2:00 PM Registration 5:00 PM Reception 6:30 PM Dinner 8:00 PM Opening Session Session 1 Keynote I: Sriram Subramaniam, Ph.D., NIH/NCI Keynote II: Alan Davidson, Ph.D., University of Toronto Monday, August 21 7:30 AM Breakfast 9:00 AM VIRUS ENTRY and EGRESS I Session 2 11:00 AM VIRUS HOST INTERACTION Session 3 12:40 PM Lunch 3:00 PM POSTER SECTION A 6:30 PM Dinner 8:00 PM DNA VIRUS STRUCTURE and ASSEMBLY I Session 4 Tuesday, August 22 7:30 AM Breakfast 9:00 AM GENOME PACKAGING I Session 5 11:00 AM Special Session: THE CRYO-EM REVOLUTION Session 6 12:30 PM Lunch 3:00 PM Free afternoon / evening Wednesday, August 23 7:30 AM Breakfast 9:00 AM RNA VIRUS STRUCTURE and ASSEMBLY I Session 7 11:00 AM GENOME PACKAGING II Session 8 12:40 PM Lunch 3:00 PM POSTER SECTION B 6:30 PM Dinner 8:00 PM DNA VIRUS STRUCTURE and ASSEMBLY II Session 9 Thursday, August 24 7:30 AM Breakfast 9:00 AM VIRUS ENTRY and EGRESS II Session 10 11:00 AM GENOME PACKAGING III Session 11 12:40 PM Lunch 2:00 PM VIRUS TECHNOLOGY Session 12 3:30 PM DNA VIRUS STRUCTURE and ASSEMBLY III Session 13 5:30 PM Award celebration / PVA Business meeting 6:30 PM Special Dinner Friday, August 25 9:00-11 AM Departure 1 XXV PVA meeting CONFERENCE PROGRAM Sunday, August 20, 2017 2:00 PM Registration 5:00 – 6:30 PM Hors D'Oeuvres Reception 6:30 – 8:00 PM Dinner 8:00 PM Opening Session Chair: Michael Rossmann (45min Talk) Keynote Speaker I: Sriram Subramaniam, Ph.D., NIH/NCI, Bethesda, MD, USA Title: High-resolution 3D electron microscopy: Progress and new opportunities (45min Talk) Keynote Speaker I: Alan Davidson, Ph.D., University of Toronto, ON, Canada Title: Phages fight back: the functioning and prevalence of anti-CRISPRs Monday, August 21, 2017 7:30 – 9:00 AM Breakfast 9:00 – 10:40 AM VIRUS ENTRY and EGRESS I Chair: Ann Palmenberg 20min Talk #1 Reza Khayat, Ph.D., City College of New York, NY, USA Title: Mechanism of cellular recognition and entry by porcine circovirus 2 20min Talk #2 Eric May, Ph.D., University of Connecticut, Storrs, CT, USA Title: Modeling Interactions of Lytic Peptides from the Non-Enveloped Flock House Virus Capsid with Biomembranes 20min Talk #3 Ian J. Molineux, Ph.D., the University of Texas at Austin, Austin, TX, USA Title: The initiation of infection by bacteriophage T7 20min Talk #4 Sarah Doore, Ph.D., Michigan State University, East Lansing, MI, USA Title: Isolation of Shigella-infecting phages from Michigan water sources 10min Talk #5 Nicholas Stone, University of Massachusetts, Worcester, MA, USA Title: Elucidating the molecular mechanisms of viral maturation using a thermophilic bacteriophage 10min Talk #6 Amber Vu, University of Iowa, Iowa City, IA, USA Title: Extragenic Suppression of a Mutation in Herpes Simplex Virus 1 UL34 That Affects Lamina Disruption and Nuclear Egress 10:40 – 11:00 AM Coffee Break 2 XXV PVA meeting 11:00 – 12:40 PM VIRUS HOST INTERACTION Chair: Paulo Tavares #1 (20min Talk) David Veesler, Ph.D., University of Washington, Seattle, WA, USA Title: A potent anti-gH/gL neutralizing antibody defines a site of vulnerability on Epstein-Barr virus #2 (20min Talk) Stefanie Barbirz, Ph.D., University of Potsdam, Potsdam, Germany Title: Host interactions of O antigen specific bacteriophages: Linking tailspike recognition to particle opening #3 (20min Talk) Sergey Buth, Ph.D., University of Texas Medical Branch, Galveston, TX, USA Title: Exopolysaccharide degrading enzymes – specificity determinants of Acinetobacter baumannii phages #4 (20min Talk) Xiaoli Xiong, Ph.D., University of Washington, Seattle, WA, USA Title: Architecture of a deltacoronavirus spike glycoprotein and its glycan shield #5 (10min Talk) #5 Nikolai Prokhorov, Ph.D., University of Texas, Galveston, TX, USA Title: Host recognition by podoviruses G7C and Alt #6 (10min Talk) #6 S. Saif Hasan, Ph.D., Purdue University, West Lafayette, IN, USA Title: A human antibody against Zika virus crosslinks the E protein to prevent infection 12:40 – 1:40 PM Lunch 3:00 – 6:00 PM POSTER SECTION A 6:30 – 8:00 PM Dinner 8:00 – 9:40 PM DNA VIRUS STRUCTURE and ASSEMBLY I Chair: Carol Teschke #1 (20min Talk) Nicola Abrescia, Ph.D., CIC bioGUNE, CIBERehd, Derio, Spain Title: Assembly of vertical single beta-barrel membrane-containing viruses by high-resolution cryo-EM #2 (20min Talk) Chuan Xiao, Ph.D., University of Texas at El Paso, El Paso, TX, USA Title: Jelly-roll spiraling all the way #3 (20min Talk) Tina Motwani, Ph.D., University of Connecticut, Storrs, CT, USA Title: Scaffolding protein: An engineer of the portal ring complex in bacteriophage P22 #4 (20min Talk) Jodi Hadden, Ph.D., University of Delaware, Newark, DE, USA Title: Insights into the structure and function of the hepatitis B virus capsid revealed by microsecond all-atom simulation #5 (10min Talk) Jason Schrad, Michigan State University, East Lansing, MI, USA Title: Staring down Titans: Characterization of the giant Samba virus #6 (10min Talk) Patricia Campbell, University of Pittsburgh, Pittsburgh, PA, USA Title: In vivo structure of the λ phage tail tube protein 3 XXV PVA meeting Tuesday, August 22, 2017 7:30 – 9:00 AM Breakfast 9:00 – 10:40 AM Shelley Grimes Memorial Session: GENOME PACKAGING I Chair: Paul Jardine #1 (20min Talk) Paul Jardine, Ph.D., University of Minnesota, Minneapolis MN, USA Title: Progress toward a complete atomic-resolution structure of bacteriophage phi29 #2 (20min Talk) Paulo Tavares, Ph.D., CNRS, Gif-sur-Yvette, France Title: The bacteriophage SPP1 portal protein headful sensor mechanism #3 (20min Talk) Michael Feiss, Ph.D., University of Iowa, Iowa City IA, USA Title: DNA Recognition and Cleavage in the -Like Phages #4 (20min Talk) Peixuan Guo, Ph.D., The Ohio State University, Columbus, Ohio, USA. Title: Motion direction control of revolving biomotors utilizing the channel chirality and arginine finger of the asymmetrical hexameric ATPase ring #5 (10min Talk) Siying Lin, The Catholic University of America, Washington, DC, USA Title: Altering the speed of a DNA packaging motor from bacteriophage T4 #6 (10min Talk) Herman Fung, Department of Biology, University of York, York, UK Title: The bacteriophage HK97 DNA packaging motor: structural and single-molecule biophysical analysis 10:40 – 11:00 AM Coffee Break 11:00 – 12:30 PM Special Session: THE CRYO-EM REVOLUTION (sponsored by FEI) Chair: James Conway #1 (30min Talk) Alasdair Steven, Ph.D., Principal Scientist, NIH/ NIAMS, Bethesda, MD, USA Title: Heterogeneity and Disorder and the Constraints Imposed in Cryo-EM of Viruses and Virus- related Specimens #2 (30min Talk) Hong Zhou, Ph.D., Principal Investigator, UCLA, USA Title: Confronting challenges in high-resolution cryo-EM: viral genomes and huge viruses #3 (30min Talk) Jeffrey Lengyel, Ph.D., Principal Scientist, Life Sciences, FEI Title: Recent technological developments in cryo-EM: Advancing challenging structural biology research problems 12:30 – 1:30 PM Lunch Free afternoon / evening 4 XXV PVA meeting Wednesday, August 23, 2017 7:30 – 9:00 AM Breakfast 9:00 – 10:40 AM RNA VIRUS STRUCTURE and ASSEMBLY Chair: David Veesler #1 (20min Talk) Michael Rossmann, Ph.D., Purdue University, West Lafayette, IN, USA Title: The structure of Zika virus #2 (20min Talk) Junjie Zhang, Ph.D., Texas A&M University, College Station, TX, USA Title: Structures of the single-stranded RNA virus Qβ reveal its MurA-binding interface and internalized coat proteins #3 (20min Talk) Xinghong Dai, Ph.D., UCLA, Los Angeles, CA, USA Title: In situ structures of the genome and genome-delivery apparatus in ssRNA phage MS2 #4 (20min Talk) Peter Stockley, Ph.D., University of Leeds, Leeds, UK Title: Nature’s ssRNA Self-assembly Instruction Manual: The Roles and Consequences of Packaging Signal-Mediated Assembly #5 (10min Talk) Lindsey Organtini, Pennsylvania State University, Hershey, PA, USA Title: Cryo-EM structures of honey bee deformed wing virus reveal conformational changes linked to genome release #6 (10min Talk) Jason Pattis, University of Connecticut, Storrs, CT, USA Title: Long Timescale Simulations of Monomeric Lassa Virus Nucleoprotein Reveals Insights into Its RNA Binding Mechanism and Ribonucleoprotein Contacts 10:40 – 11:00 AM Coffee Break 11:00 – 12:40 PM GENOME PACKAGING II Chair: Doug Smith #1 (20min Talk) Stephen Harvey, Ph.D., University of Pennsylvania, Philadelphia, PA, USA Title: Electrostatically-Driven DNA Conformational Changes Drive Genome Packaging in dsDNA Bacteriophages: Evidence from Computational and Theoretical Studies #2 (20min Talk) Susan Schroeder Ph.D., University of Oklahoma, Norman, OK, USA Title: NMR structures and dynamics in a prohead RNA loop that binds metal ions #3 (20min Talk) Peng Jing, Ph.D., Indiana-Purdue University, Fort Wayne, IN, USA Title: The Flashing Brownian Ratchet in the Connector at the Late Stage of DNA Packaging in the Bacteriophage Phi29 #4 (20min Talk) Allen Eastlund, Ph.D., University of Minnesota, Minneapolis, MN, USA Title: Disruption of a pentameric ring motor through molecular and substrate substitution #5 (10min Talk) Wei-Chun Tang, the Catholic University of America, Washington, DC, USA Title: The bacteriophage T4 portal is a signal transducer of the DNA packaging machine #6 (10min Talk) Eva Weiss, University of York, York, UK 5 XXV PVA meeting Title: Phylogeny of viral strains based on packaging signal profiles 12:40 – 1:40 PM Lunch 3:00 – 6:00 PM POSTER SECTION B 6:30 – 8:00 PM Dinner 8:00 – 9:40 PM DNA VIRUS STRUCTURE and ASSEMBLY II Chair: Susan Hafenstein #1 (20min Talk) Cécile Breyton,