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Contact email: [email protected] Contact skype: live:linda_8965 Oxandrolone (Anavar) Stanozolol (Winstrol) HOT Sell Oxymetholone (Anadrol) Dianabol(Methandrostenolone) Test Base (No ester) Test Undecanoate Test Enanthate 17-methyltestosterone Test Propionate 17α-Methyl-1-Testosterone Test Phenylpropionate Oral turinabol Testosterone Test Isocaproate Turinabol Test Cypionate Mesterolone Test Sustanon 250 Fluoxymesterone Mestanolone(MDHT) Stanolone (DHT) Nandrolone Decanoate/DECA Nandrolone undecanoate Nandrolone Nandrolone Phenypropionate Nandrolone cypiontate Boldenone base Boldenone Boldenone Undecylenate(Equipoise) Boldnone cypiontate Trenbolone base Trenbolone acetate (Finaplix) Trenbolone Trenbolone enanthate (Parabolan) Trenbolone cyclohexylmethylcarbonate Methyltrienolone (Metribolone) Drostanolone Propionate (Masteron p) Drostanolone Drostanolone Enanthate (Masteron) Superdrol Powder (methyl-drostanolone) Methasterone Methenolone Methenolone Enanthate Methenolone Acetate Tibolone Dehydroepiandrosterone/DHEA Mibolerone 7-Keto- DHEA Others Methylstenbolone Triamcinolone Dehydronandrolon Finasteride L-Thyroxine, T4 Cytomel, T3 Tamoxifen Citrate (Nolvadex) DL-Aminoglutethimide 1,4,6-Androstatrien-3,17-dione Clomiphene citrate (Clomid) Anti-Estrogen (ATD) Exemestane(Aromasin) SERMs and SARMs Anastrozole (Arimidex) Letrazole/Femara/ Formestane Androstenedione Dimethazine Pro hormone Epistane (Epithio / Havoc) (Epitiostanol) Methoxygonadiene ( Max LMG) (Furazabol) Methoxydienone (Methoxygonadiene) Norandrostenedione/19-nor-4-androstene-3;Estr-4-ene-3, Orastan-A (Furazabol THP) Orastan-E (Stanozol THP) Superdrol (Methyldrostanolone) Trenavar/ Trendione/ Celtitren 1,4-AD (Boldione) (Androstadienedione) Methyl 1,4-AD (Methyl-1,4-androstadienediol) 4-Androstenediol ( 4-AD ) 6-OXO (4-Androstenetrione) 11-OXO (11-Ketotestosterone/ Adrenosterone ) 17a-Methyl-1-testosterone ( M1T) 19 Tren X (Methyldienedione) (Estra-4,9-diene-3,17-dione) Cialis (Tadalafil) Acetildenafil (Hongdenafil) Sex Enhance Viagra (Sildenafil) Yohimbine Hcl vardenafil (Levitra) Dapoxetine Benzocaine Lidocaine Procaine Tetracaine Pain Killer Phenacetin Gamma-Butyrolactone /GBL.

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Expression of Recombinant Human Androgen Receptor and Its Use for Screening Methods
Institut für Physiologie FML Weihenstephan Technische Universität München Expression of recombinant human androgen receptor and its use for screening methods Ellinor Rose Sigrid Bauer Vollständiger Abdruck der von der Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt der Technischen Universität München zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften genehmigten Dissertation. Vorsitzender: Univ.-Prof. Dr. B. Hock Prüfer der Dissertation: Univ.-Prof. Dr. H. H. D. Meyer Univ.-Prof. Dr. H. Sauerwein (Rheinische Friedrich-Wilhelms-Universität Bonn) Die Dissertation wurde am 31.10.2002 bei der Technischen Universität München eingereicht und durch die Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt am 03.12.2002 angenommen. Introduction Content 1. INTRODUCTION ..................................................................................................................................... 5 1.1. ENDOCRINE DISRUPTERS 5 1.2. ANDROGENS AND ANTIANDROGENS 7 1.2.1. DEFINITIONS 7 1.2.2. MODE OF ACTION 8 1.3. STRUCTURES OF ENDOCRINE DISRUPTERS 10 1.4. STRATEGIES FOR MONITORING ANDROGEN ACTIVE SUBSTANCES 13 1.4.1. IN VIVO METHODS 13 1.4.2. IN VITRO METHODS 15 1.5. OBJEKTIVE OF THE STUDIES 18 2. MATERIALS AND METHODS ................................................................................................................. 19 2.1. PREPARATION OF RECEPTORS 19 2.2. ASSAY SYSTEMS 19 2.2.1. IN SOLUTION AR ASSAY 19 2.2.2. IMMUNO-IMMOBILISED RECEPTOR ASSAY (IRA) 20 2.2.3. PR AND SHBG ASSAYS 21 2.2.4. DATA EVALUATION 21 2.3. ANALYTES 22 3. RESULTS AND DISCUSSION ................................................................................................................. 23 3.1. DEVELOPMENT OF NEW ASSAY SYSTEMS 23 3.1.1. BAR ASSAY 23 3.1.2. CLONING OF THE HUMAN AR AND PRODUCTION OF FUNCTIONAL PROTEIN 24 3.1.3. DEVELOPMENT OF A SCREENING ASSAY ON MICROTITRE PLATES (IRA) 25 3.2.
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(Cyp19a1b-GFP) Zebrafish Embryos
Screening Estrogenic Activities of Chemicals or Mixtures In Vivo Using Transgenic (cyp19a1b-GFP) Zebrafish Embryos Franc¸ois Brion1, Yann Le Page2, Benjamin Piccini1, Olivier Cardoso1, Sok-Keng Tong3, Bon-chu Chung3, Olivier Kah2* 1 Unite´ d’Ecotoxicologie in vitro et in vivo, Direction des Risques Chroniques, Institut National de l’Environnement Industriel et des Risques (INERIS), Verneuil-en- Halatte, France, 2 Universite´ de Rennes 1, Institut de Recherche Sante´ Environnement & Travail (IRSET), INSERM U1085, BIOSIT, Campus de Beaulieu, Rennes France, 3 Taiwan Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan Abstract The tg(cyp19a1b-GFP) transgenic zebrafish expresses GFP (green fluorescent protein) under the control of the cyp19a1b gene, encoding brain aromatase. This gene has two major characteristics: (i) it is only expressed in radial glial progenitors in the brain of fish and (ii) it is exquisitely sensitive to estrogens. Based on these properties, we demonstrate that natural or synthetic hormones (alone or in binary mixture), including androgens or progestagens, and industrial chemicals induce a concentration-dependent GFP expression in radial glial progenitors. As GFP expression can be quantified by in vivo imaging, this model presents a very powerful tool to screen and characterize compounds potentially acting as estrogen mimics either directly or after metabolization by the zebrafish embryo. This study also shows that radial glial cells that act as stem cells are direct targets for a large panel of endocrine disruptors, calling for more attention regarding the impact of environmental estrogens and/or certain pharmaceuticals on brain development. Altogether these data identify this in vivo bioassay as an interesting alternative to detect estrogen mimics in hazard and risk assessment perspective.
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PROJECT REVIEW “Characterization of the main metabolites of 17-methylstenblone and 17 methylmethenolone produced by human hepatocytes and liver fractions” Prof C. Ayotte, (INRS-Institut Armand-Frappier, Canada) New steroids openly appear on the market in products labelled with a rather confusing nomenclature. Once characterized, pharmaceutical grade products not being available, knowledge of the biotransformation pathways essential to an efficient detection of utilization by athletes is difficult to gain since administration to human volunteers should be restricted to the minimum. The alternative is a reliable in vitro model. Human hepatocytes, fresh or cryopreserved are now available commercially. We have successfully produced and identified phase I metabolites from incubations of human hepatocytes with different steroids, such as 17-methyldrostanolone and desoxymethyltestosterone (DMT). The aim of this project is to produce in vitro from human hepatocytes and liver fractions the metabolites of two steroids, the 17-methylated derivatives of stenbolone and its isomer methenolone. The principal metabolites will be synthesized and characterized by NMR and mass spectrometry. The characterization of metabolites will enable the identification of markers of utilization to be incorporated in routine testing methods. The approach for the chemical synthesis of metabolites will be shared with NMI insuring the distribution to other doping control laboratories. Improving the knowledge of steroid biotransformation is a further benefit from these studies. Characterization of 17-Methylstenbolone and 17-Methylmethenolone and Identification of Metabolites Produced by Human Hepatocytes and Liver Fractions WADA Project no. 11A16CA Christiane Ayotte, Philippe Räss, Alexandre Sylvestre, INRS-Institut Armand-Frappier Summary We have synthesized and characterized two designer steroids, 17α-methylmethenolone and 17α- methylstenbolone; the latter is proposed on the internet and two groups have reported different and contradictory results.
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Vargas KEA, Et Al. Hepatotoxicity Associated with Methylstenbolone and Copyright© Vargas KEA, Et Al
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A10 Anabolic Steroids Hardcore Info
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( 12 ) United States Patent
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Blackstone Indictment
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Order in Council 1243/1995
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