Recombinant Human LRG1 Protein Catalog Number: ATGP3755

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Recombinant human LRG1 protein Catalog Number: ATGP3755 PRODUCT INPORMATION Expression system Baculovirus Domain 36-347aa UniProt No. P02750 NCBI Accession No. NP_443204 Alternative Names Leucine-rich alpha-2-glycoprotein, LRG1, HMFT1766, LRG PRODUCT SPECIFICATION Molecular Weight 35.4 kDa (321aa) Concentration 0.5mg/ml (determined by absorbance at 280nm) Formulation Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol Purity > 95% by SDS-PAGE Endotoxin level < 1 EU per 1ug of protein (determined by LAL method) Tag His-Tag Application SDS-PAGE Storage Condition Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. BACKGROUND Description LRG1, also known as leucine-rich alpha-2-glycoprotein, is a member of leucine-rich repeat (LRR) family. It is neutrophilic expressed during granulocyte differentiation. It has been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. It binds directly to the TGF-beta accessory receptor endoglin, which, in the presence of TGF-beta1, results in promotion of the pro-angiogenic Smad1/5/8 1 Recombinant human LRG1 protein Catalog Number: ATGP3755 signaling pathway. It promotes proliferation and inhibits apoptosis in colorectal cancer cells via RUNX1 activation. It is a key physiological regulator of dendrite complexity of hippocampal pyramidal neurons. It physically interacts with TrkB and attenuates BDNF signaling. Recombinant human LRG1, fused to His-tag at C- terminus, was expressed in insect cell and purified by using conventional chromatography techniques. Amino acid Sequence ADPVTLSPKD CQVFRSDHGS SISCQPPAEI PGYLPADTVH LAVEFFNLTH LPANLLQGAS KLQELHLSSN GLESLSPEFL RPVPQLRVLD LTRNALTGLP PGLFQASATL DTLVLKENQL EVLEVSWLHG LKALGHLDLS GNRLRKLPPG LLANFTLLRT LDLGENQLET LPPDLLRGPL QLERLHLEGN KLQVLGKDLL LPQPDLRYLF LNGNKLARVA AGAFQGLRQL DMLDLSNNSL ASVPEGLWAS LGQPNWDMRD GFDISGNPWI CDQNLSDLYR WLQAQKDKMF SQNDTRCAGP EAVKGQTLLA VAKSQHHHHH H General References Wang X., et al. (2013) Nature. 499:306-311. O Donnell LC., et al. (2002) J Leukoc Biol. 72:478-485. DATA SDS-PAGE 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. 2.

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LRG1 Promotes Diabetic Kidney Disease Progression by Enhancing TGF-B–Induced Angiogenesis
BASIC RESEARCH www.jasn.org LRG1 Promotes Diabetic Kidney Disease Progression by Enhancing TGF-b–Induced Angiogenesis Quan Hong,1,2 Lu Zhang,3,1 Jia Fu,1 Divya A. Verghese,1 Kinsuk Chauhan,1 Girish N. Nadkarni,1 Zhengzhe Li,1 Wenjun Ju,4 Matthias Kretzler ,4 Guang-Yan Cai,2 Xiang-Mei Chen,2 Vivette D. D’Agati,5 Steven G. Coca ,1 Detlef Schlondorff,1 John C. He,1,6 and Kyung Lee1 Due to the number of contributing authors, the affiliations are listed at the end of this article. ABSTRACT Background Glomerular endothelial dysfunction and neoangiogenesis have long been implicated in the pathogenesis of diabetic kidney disease (DKD). However, the specific molecular pathways contributing to these processes in the early stages of DKD are not well understood. Our recent transcriptomic profiling of glomerular endothelial cells identified a number of proangiogenic genes that were upregulated in diabetic mice, including leucine-rich a-2-glycoprotein 1 (LRG1). LRG1 was previously shown to promote neovascularization in mouse models of ocular disease by potentiating endothelial TGF-b/activin receptor- like kinase 1 (ALK1) signaling. However, LRG1’s role in the kidney, particularly in the setting of DKD, has been unclear. Methods We analyzed expression of LRG1 mRNA in glomeruli of diabetic kidneys and assessed its localization by RNA in situ hybridization. We examined the effects of genetic ablation of Lrg1 on DKD progression in unilaterally nephrectomized, streptozotocin-induced diabetic mice at 12 and 20 weeks after diabetes induction. We also assessed whether plasma LRG1 was associated with renal outcome in patients with type 2 diabetes.
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LRG1 (C-4): Sc-390920
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